genomewide characterization of host-pathogen interactions by ...

Maren Depke
C O N T E N T S
GENOMEWIDE CHARACTERIZATION OF HOST-PATHOGEN INTERACTIONS
BY TRANSCRIPTOMIC APPROACHES 1
CONTENTS 3
ZUSAMMENFASSUNG DER DISSERTATION 5
SUMMARY OF DISSERTATION 9
INTRODUCTION 13
INFECTIOUS DISEASES AND IMMUNE SYSTEM 13
Aspects of Innate Immunity 13
Aspects of Adaptive Immunity 16
Modulation of Immune Reactions 18
STAPHYLOCOCCUS AUREUS 20
General Features of S. aureus 20
S. aureus, a Commensal and Opportunistic Pathogen 21
S. aureus Virulence Factors 22
Mechanisms of S. aureus Adaptation to its Environment 28
Increasing Importance and Danger of S. aureus Infections 29
STUDIES OF HOST-PATHOGEN INTERACTIONS 31
Model Systems for Studies of Host Reactions Potentially Influencing the Outcome of Infections 31
Model Systems for Studies of Host-Pathogen Interactions 35
Questions and Aims of the Studies Described in this Thesis 37
MATERIAL AND METHODS 39
LIVER GENE EXPRESSION PATTERN IN A MOUSE PSYCHOLOGICAL STRESS MODEL 39
KIDNEY GENE EXPRESSION PATTERN IN AN IN VIVO INFECTION MODEL 43
GENE EXPRESSION PATTERN OF BONE-MARROW DERIVED MACROPHAGES AFTER INTERFERON-GAMMA TREATMENT 47
HOST CELL GENE EXPRESSION PATTERN IN AN IN VITRO INFECTION MODEL 51
PATHOGEN GENE EXPRESSION PROFILING 55
Growth Media Comparison Study 55
In vitro Infection Experiment Study 57
Tiling Array Expression Profiling 60
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