genomewide characterization of host-pathogen interactions by ...

Maren Depke
Acknowledgments
Kidney gene expression pattern in an in vivo infection model
The mouse infection model was established and performed in the setting of the DFG
Sonderforschungsbereich SFB TR 34 “Pathophysiologie von Staphylokokken in der Post-Genom
Ära” by Tina Schäfer and Knut Ohlsen, who also contributed to study conception and design.
Marc Burian co-operated in the wet-lab-work for the second biological replicate of infected
samples and in the final data analysis and interpretation. The strain S. aureus RN1HG was
generated in the lab of Friedrich Götz and its isogenic sigB mutant by Jan Pané-Farré in the lab of
Michael Hecker. The work was supervised by Uwe Völker.
Tina Schäfer, Knut Ohlsen (Institute of Molecular Infection Biology, Julius-Maximilians University of
Würzburg, Germany)
Jan Pané-Farré, Susanne Engelmann, Michael Hecker (Institute of Microbiology, Ernst-Moritz-Arndt
University of Greifswald, Germany)
Friedrich Götz (Department of Microbial Genetics, Eberhards-Karls University of Tübingen, Germany)
Marc Burian, Uwe Völker (Interfaculty Institute for Genetics and Functional Genomics, Department of
Functional Genomics, Ernst-Moritz-Arndt University of Greifswald, Germany)
Gene expression pattern of bone-marrow derived macrophages after interferon-γ activation
Bone-marrow derived macrophages were generated and stimulated with IFN-γ by Kathrin
Breitbach. Proteome analysis was conducted by Dinh Hoang Dang Khoa. The study was
performed in the setting of the DFG Sonderforschungsbereich SFB TR 34 “Pathophysiologie von
Staphylokokken in der Post-Genom Ära”. The work was supervised by Uwe Völker.
Kathrin Breitbach, Antje Bast, Ivo Steinmetz (Institute of Medical Microbiology, Ernst-Moritz-Arndt
University of Greifswald, Germany)
Dinh Hoang Dang Khoa, Lars Brinkmann, Manuela Gesell Salazar, Elke Hammer, Leif Steil, Frank
Schmidt, Uwe Völker (Interfaculty Institute for Genetics and Functional Genomics, Department of
Functional Genomics, Ernst-Moritz-Arndt University of Greifswald, Germany)
Host cell gene expression pattern in an in vitro infection model
Infection experiments were performed in close collaboration with Melanie Gutjahr, who
worked on host proteome analysis and additionally accomplished control measurements and cell
culture. Petra Hildebrandt conducted FACS cell sorting. The strain S. aureus RN1HG was
generated in the lab of Friedrich Götz and its GFP expressing variant RN1HG pMV158GFP by
Leonard Menschner in the lab of Michael Hecker. The work was supervised by Uwe Völker. This
study was performed in the setting of the DFG Sonderforschungsbereich SFB TR 34
“Pathophysiologie von Staphylokokken in der Post-Genom Ära”.
Leonhard Menschner, Susanne Engelmann, Michael Hecker (Institute of Microbiology, Ernst-Moritz-
Arndt University of Greifswald, Germany)
Melanie Gutjahr, Petra Hildebrandt, Elke Hammer, Uwe Völker (Interfaculty Institute for Genetics and
Functional Genomics, Department of Functional Genomics, Ernst-Moritz-Arndt University of Greifswald,
Germany)
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