genomewide characterization of host-pathogen interactions by ...

mean normalized intensity
mean normalized intensity
mean normalized intensity
mean normalized intensity
mean normalized intensity
mean normalized intensity
Maren Depke
Results
Pathogen Gene Expression Profiling
A
B
45 37 34
98 75 43
C
internalization-specific
differential expression;
induction 2.5 h after
start of infection
internalization-specific
differential expression;
induction 6.5 h after
start of infection
F
internalization-specific
differential expression;
repression 2.5 h after
start of infection
internalization-specific
differential expression;
repression 6.5 h after
start of infection
10E+02
10E+02
10E+01 10.00
10E+01 10.00
10E+00 1.00
10E+00 1.00
10E-01 0.10
10E-01 0.10
D
10E-02 0.01
1 2 3 4 5 6 7
exp.
1 h
co.
2.5 h
int.
6.5 h
int.
2.5 h
co.
6.5 h
co.
2.5 h
anae.
S. aureus RN1HG sample conditions and time points
G
10E-02 0.01
1 2 3 4 5 6 7
exp.
1 h
co.
2.5 h
int.
6.5 h
int.
2.5 h
co.
6.5 h
co.
2.5 h
anae.
S. aureus RN1HG sample conditions and time points
10E+02
10E+02
10E+01 10.00
10E+01 10.00
10E+00 1.00
10E+00 1.00
10E-01 0.10
10E-01 0.10
E
10E-02 0.01
10E-02 0.01
10E+02
1 2 3 4 5 6 7
exp.
1 h
co.
2.5 h
int.
6.5 h
int.
2.5 h
co.
6.5 h
co.
2.5 h
anae.
S. aureus RN1HG sample conditions and time points
H
10E+02
1 2 3 4 5 6 7
exp.
1 h
co.
2.5 h
int.
6.5 h
int.
2.5 h
co.
6.5 h
co.
2.5 h
anae.
S. aureus RN1HG sample conditions and time points
10E+01 10.00
10E+01 10.00
10E+00 1.00
10E+00 1.00
10E-01 0.10
10E-01 0.10
10E-02 0.01
10E-02 0.01
1 2 3 4 5 6 7
exp.
1 h
co.
2.5 h
int.
6.5 h
int.
2.5 h
co.
6.5 h
co.
2.5 h
anae.
S. aureus RN1HG sample conditions and time points
1 2 3 4 5 6 7
exp.
1 h
co.
2.5 h
int.
6.5 h
int.
2.5 h
co.
6.5 h
co.
2.5 h
anae.
S. aureus RN1HG sample conditions and time points
Fig. R.5.35: Internalization-specific differential gene expression.
A, B. Overview on the number of sequences which were differentially expressed in internalized staphylococci in comparison to all
other control samples and exhibited induction (A) or repression (B).
C-H. Overview on mean normalized gene expression intensity. After the global normalization by inter-chip scaling and detrending,
each individual gene has been normalized to the expression level of the baseline sample “1 h serum/CO 2 control” (1 h co.). Although
not being continuous data, values were depicted as line graphs instead of bar charts for better facility of inspection.
Intensity values are depicted for 45 sequences specifically induced after 2.5 h (C), 37 sequences specifically induced at both time
points (D), 34 sequences specifically induced after 6.5 h (E), 98 sequences specifically repressed after 2.5 h (F), 75 sequences
specifically repressed at both time points (G), and 43 sequences specifically repressed after 6.5 h (H).
(exp. − exponential growth phase; 1 h co. – 1 h serum/CO 2 control; 2.5 h int. − 2.5 h internalization; 6.5 h int. − 6.5 h internalization;
2.5 h co. − 2.5 h serum/CO 2 control; 6.5 h co. − 6.5 h serum/CO 2 control; 2.5 h anae. − 2.5 h anaerobic incubation)
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