genomewide characterization of host-pathogen interactions by ...

Maren Depke
Results
Gene Expression Pattern of Bone-Marrow Derived Macrophages after Interferon-gamma Treatment
Table R.3.15: Strain differences in gene expression of plasma membrane receptors between BMM of BALB/c and C57BL/6 mice.
gene
name
description
Rosetta Resolver Annotation fold change a IFN-γ effect
alias
Entrez
Gene ID
control IFN-γ BALB/c C57BL/6
Cadm1 cell adhesion molecule 1
Bl2, ST17, Igsf4, Necl2, Tslc1,
Igsf4a, RA175A, RA175B, RA175C, 54725 -4.7 -4.0
RA175N, SgIGSF, SynCam
L1cam L1 cell adhesion molecule L1, CD171, L1-NCAM, NCAM-L1 16728 -2.2 -2.2
Pcdh7 protocadherin 7 54216 -1.7 -1.7 x
Itgal integrin alpha L Cd11a, LFA-1, Ly-15, Ly-21 16408 2.0 1.4 x x
Cd14 CD14 antigen 12475 -3.2 -2.4
Cd40 CD40 antigen
IGM, p50, Bp50, GP39, IMD3,
TRAP, HIGM1, T-BAM, Tnfrsf5
21939 -1.2 -2.4 x x
Fcgr1 Fc receptor, IgG, high affinity I CD64, IGGHAFC, FcgammaRI 14129 -2.0 -1.9 x x
Tlr1 toll-like receptor 1 21897 -2.0 -1.6
Procr protein C receptor, endothelial Ccca, EPCR 19124 2.3 2.3
a Fold change values were calculated for the comparison of C57BL/6 BMM with the baseline of BALB/c BMM of mean values from
three biological replicates per group. Positive values indicate higher expression in C57BL/6 BMM than in BALB/c BMM, whereas
negative values indicate higher expression in BALB/c BMM than in C57BL/6 BMM. Differential expression between BMM of the two
strains at the same treatment level (IFN-γ treated or medium control) using statistical testing in combination with an absolute fold
change cutoff of 1.5 is indicated in bold.
In the group of plasma membrane receptors, the adhesion molecules Cadm1, L1cam, and
Pcdh7 were higher expressed in BALB/c BMM, whereas expression of integrin Itgal was higher in
C57BL/6 BMM. The expression of CD14 / LPS receptor, CD40 / co-stimulatory receptor, Fcgr1 / high
affinity IgG Fc receptor I, and Tlr1 / toll-like receptor 1, was higher in BALB/c BMM than in
C57BL/6 BMM. Contrarily, Procr / protein C receptor exhibited higher expression in C57BL/6 BMM
(Table R.3.15).
Table R.3.16: Strain differences in gene expression of immune response related proteins, lysosomal, extracellular matrix degrading,
and detoxification enzymes between BMM of BALB/c and C57BL/6 mice.
Rosetta Resolver Annotation fold change a IFN-γ effect
gene
Entrez
description
alias
name
Gene ID
control IFN-γ BALB/c C57BL/6
Lbp lipopolysaccharide binding protein Ly88 16803 1.5 2.3
Lta4h leukotriene A4 hydrolase 16993 1.9 1.7
Oas2 2'-5' oligoadenylate synthetase 2 Oasl11 246728 -2.6 -2.5 x
Oasl1 2'-5' oligoadenylate synthetase-like 1 oasl9 231655 -2.5 -4.9 x
Arg2 arginase type II AII 11847 -5.1 -3.7
Tfpi tissue factor pathway inhibitor EPI, LACI 21788 2.0 2.1
Ctsc cathepsin C DPP1, DPPI 13032 1.5 1.7 x x
Ctse cathepsin E CE, CatE 13034 -4.3 -5.7
Ctsh cathepsin H 13036 2.7 2.0 x
Hyal1 hyaluronoglucosaminidase 1 Hya1, Hyal-1 15586 -2.2 -1.7
Mmp14 matrix metallopeptidase 14 (membrane-inserted)
MT1-MMP, MT-
MMP-1
17387 2.3 1.8
Gstm2 glutathione S-transferase, mu 2 Gstb2, Gstb-2 14863 3.5 3.3
Gstp1 glutathione S-transferase, pi 1 GstpiB 14870 1.9 2.0
a Fold change values were calculated for the comparison of C57BL/6 BMM with the baseline of BALB/c BMM of mean values from
three biological replicates per group. Positive values indicate higher expression in C57BL/6 BMM than in BALB/c BMM, whereas
negative values indicate higher expression in BALB/c BMM than in C57BL/6 BMM. Differential expression between BMM of the two
strains at the same treatment level (IFN-γ treated or medium control) using statistical testing in combination with an absolute fold
change cutoff of 1.5 is indicated in bold.
LBP, LPS binding protein, and Lta4h, whose gene product catalyzes the first reaction step in
the biosynthesis of leukotrienes, i. e. the reaction from leukotriene A4 to leukotriene B4, were
higher expressed in C57BL/6 BMM than in BALB/c BMM. In contrast, 2'-5'-oligoadenylate
synthetase genes Oas2 and Oasl1, which are part of the innate immune response, exhibited
107