biological sciences HONOURs 2014 - The University of Sydney
biological sciences HONOURs 2014 - The University of Sydney
biological sciences HONOURs 2014 - The University of Sydney
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
SMALL RNAs AND<br />
BIOFACTORIES<br />
49<br />
Research Interests<br />
RNA interference (RNAi), was discovered and described by<br />
our group, in plants, in the late 1990s and has revolutionised<br />
plant and animal research. <strong>The</strong> technology gives researchers<br />
the ability to silence almost any gene, at will, and works by redirecting<br />
an intrinsic RNA-degrading mechanism that is present<br />
in almost all eukaryotic cells. We now know that the mechanism<br />
not only provides defence against viruses but also regulates<br />
patterns <strong>of</strong> development and epigenetics. <strong>The</strong> main players in<br />
this pathway are Dicers, Argonautes and a suite <strong>of</strong> small (s)<br />
RNAs.<br />
We have been studying the different sRNA pathways to<br />
elucidate their components and how they operate and<br />
are currently studying a family <strong>of</strong> proteins (called DRBs)<br />
that we believed would discriminate between the different<br />
Dicerproduced sRNAs and transfer them to the appropriate<br />
Argonautes. Some <strong>of</strong> these proteins are behaving as predicted,<br />
others are showing interesting and unexpected properties that<br />
hint at the possibility <strong>of</strong> other twists to the RNA-mediated<br />
developmental-control pathway.<br />
We have ongoing research examining the nature <strong>of</strong> a mobile<br />
silencing signal, the basis <strong>of</strong> epigenetic gene regulation<br />
(including transgenerational epigenetic inheritance), and how other non-coding RNAs are<br />
mediating genome regulation. A recent direction that we are taking, and for which we were<br />
awarded an ARC “Super Science” grant, is the use <strong>of</strong> Native Australian Nicotiana species<br />
as bi<strong>of</strong>actories for the rapid and high level production <strong>of</strong> valuable proteins (e.g. vaccines,<br />
antibodies, and other therapeutic agents). In conjunction with this we are sequencing the entire<br />
genome and transcriptome <strong>of</strong> Nicotiana benthamiana to identify why it is such a special plant for<br />
the rapid expression <strong>of</strong> foreign proteins.<br />
To get a greater impression <strong>of</strong> the work we do, visit our group genome website<br />
sydney.edu.au/science/molecular_bioscience/sites/benthamiana/<br />
Pr<strong>of</strong>essor Peter<br />
Waterhouse<br />
Room 202, Macleay<br />
Building A12<br />
T: (02) 9114 0745<br />
E: peter.waterhouse@<br />
sydney.edu.au