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biological sciences HONOURs 2014 - The University of Sydney

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SMALL RNAs AND<br />

BIOFACTORIES<br />

49<br />

Research Interests<br />

RNA interference (RNAi), was discovered and described by<br />

our group, in plants, in the late 1990s and has revolutionised<br />

plant and animal research. <strong>The</strong> technology gives researchers<br />

the ability to silence almost any gene, at will, and works by redirecting<br />

an intrinsic RNA-degrading mechanism that is present<br />

in almost all eukaryotic cells. We now know that the mechanism<br />

not only provides defence against viruses but also regulates<br />

patterns <strong>of</strong> development and epigenetics. <strong>The</strong> main players in<br />

this pathway are Dicers, Argonautes and a suite <strong>of</strong> small (s)<br />

RNAs.<br />

We have been studying the different sRNA pathways to<br />

elucidate their components and how they operate and<br />

are currently studying a family <strong>of</strong> proteins (called DRBs)<br />

that we believed would discriminate between the different<br />

Dicerproduced sRNAs and transfer them to the appropriate<br />

Argonautes. Some <strong>of</strong> these proteins are behaving as predicted,<br />

others are showing interesting and unexpected properties that<br />

hint at the possibility <strong>of</strong> other twists to the RNA-mediated<br />

developmental-control pathway.<br />

We have ongoing research examining the nature <strong>of</strong> a mobile<br />

silencing signal, the basis <strong>of</strong> epigenetic gene regulation<br />

(including transgenerational epigenetic inheritance), and how other non-coding RNAs are<br />

mediating genome regulation. A recent direction that we are taking, and for which we were<br />

awarded an ARC “Super Science” grant, is the use <strong>of</strong> Native Australian Nicotiana species<br />

as bi<strong>of</strong>actories for the rapid and high level production <strong>of</strong> valuable proteins (e.g. vaccines,<br />

antibodies, and other therapeutic agents). In conjunction with this we are sequencing the entire<br />

genome and transcriptome <strong>of</strong> Nicotiana benthamiana to identify why it is such a special plant for<br />

the rapid expression <strong>of</strong> foreign proteins.<br />

To get a greater impression <strong>of</strong> the work we do, visit our group genome website<br />

sydney.edu.au/science/molecular_bioscience/sites/benthamiana/<br />

Pr<strong>of</strong>essor Peter<br />

Waterhouse<br />

Room 202, Macleay<br />

Building A12<br />

T: (02) 9114 0745<br />

E: peter.waterhouse@<br />

sydney.edu.au

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