Anthelmintic activity of Cassia auriculata L. extracts-In vitro study

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Sucheta A Gaikwad et al J. Nat. Prod. Plant Resour., 2011, 1 (2): 62-66 ______________________________________________________________________________ RESULTS AND DISCUSSION In conclusion, this study suggests that the plants used by tribals traditionally to treat intestinal worm infections, showed significant anthelmintic activity. The experimental evidence obtained in the laboratory model could provide a rationale for the traditional use of these plants as anthelmintic. The extracts of leaves displays a significant anthelmintic activity in dose dependent manner ( TableI). The anthelmintic activity of ethanolic and quath extracts was comparable with that of standard drug. Albendazole, this drug is effective in a broad range of helminth infections, including round worms, hookworms, whipworms, pinworms and animal side effects. The drug is poorly absorbed and efficacy depends on transit time in the gastrointestinal tract. The toxicity is extremely low, but the drug has not been studied in children under two years of age. Gastro intestinal disturbances may occur during expulsion of the worms, the mechanism of action involves inhibition of the glucose uptake system leading to a lethal depletion of energy reserves in the helminthes. Phytochemical screening of the crude extracts reveales the presence of flavonoids and polyphenolic compound as one of the major chemical constituents. It is possible that tannins contained in the extracts of Cassia auriculata L produced similar effects. Graph I Shows Comparative study on anthelmintic activity of Cassia auriculata L. Extracts on adult Indian earthworm. Time (Min) Treatments Table II Shows Statistical varience of analysis (ANOVA) on anthelmintic activity of Cassia auriculata L. Extracts Source of Variation SS df MS F P-value F crit Between groups 14117.74 4 3529.4 5.731 0.01158 5.994 Within groups 6158 10 615.8 Total 20275.74 14 Each value represents mean ±SEM(N=6).Data are found to be significant by testing through two way ANOVA with replication at 1% level of significance. Scholar Research Library 65
Sucheta A Gaikwad et al J. Nat. Prod. Plant Resour., 2011, 1 (2): 62-66 ______________________________________________________________________________ CONCLUSION It is concluded based on the findings of the present study that the leaves of Cassia auriculata L. possess varying degree of anthelmintic activities. The dose of the extract is increased, a gradual increase in the activity of extracts is dose dependent.(GraphI) Quath and ethanol extracts exhibited significant anthelmintic activity at C.auriculata highest concentration of 20 mg/ml. This study strongly supports the traditional use of leaves as anthelmintic. Acknowledgement The authors are thankful to the Principal, S.P.College and Head, Department of Chemistry, S.P.College, Pune-30 for providing experimentation facilities. REFERENCES [1] William o Foye, Principles of Medicinal Chemistry, Varghese Publishing House, Bombay, Third Edition year pg 726-730 [2] Aswar Manoj, Aswar Urmila,Watkar Bhagyashri.,et al. Int J Green Pharm,170-173,( 2008) [3]Bundy D. A., Trans Royal Soc Trop Med Hyg, 1994, 8, 259-261. [4]Tagbota S, Townson S., Adv Parasitol, 2001, 50,199-205. [5]Sondhi, S.M., Shahu, R., Magan Archana, Indian Drugs, 1994, 31(7), 317-320. [6]Nadkarni A.K., Indian Material Medica, Popular Prakashan Pvt. Ltd. Bombay., 1954, Vol. I, 168. [7]Satish B. Kosalge, Ravindra A. Fursule, International Journal of Pharm Tech Research Vol.1,No.1,pp 68-72, Jan – March 2009 [8]Dash G. K., Suresh P, Kar D. M., Ganpaty S., Panda S. B., J Nat Rem, 2002, 2, 182-185 [9]Bate-Smith EC., J Soc Bot, 58: 95-103,(1962). [10]Martin RJ., Vet J, 154:11-34, 1997 [11]Mali R.G., Wadekar R.R., Indian J Pharm Sci. 2008, Jan-Feb, 131-133 [12].Kokate CK.Practical Pharmacognosy, 4 th edn, Vallabha Prakashan, New Delhi, 149-156, (1999). [13]Khandelwal KR. Practical Pharmacognosy Technique and Experiments, 2nd edn, Nirali Prakashan, Pune, 149-56, (2000). [14] Ajaiyeoba EO, Onocha PA, OlarenwajuOT., Pharm Biol, 39:217- 220,(2001). [15] Deore SL, Khadabadi SS, Kamdi KS. et al. Int J Chem Tech Res, 1: 177-179,(2009).. Scholar Research Library 66
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Anthelmintic activity of Cassia auriculata L. extracts-In vitro study

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