chapter 3 - RiuNet

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TABLE OF CONTENTS SUMMARY 1 RESUMEN 3 RESUM 5 GENERAL INTRODUCTION 7 1. A biological overview of goal species 9 1.1 European eel 9 1.2 Pufferfish 2. 11 2. Sperm phisiology in fish 12 2.1 Factors determining sperm activation 12 2.2 Role 3. of ions during sperm activation 14 3. Sperm quality in fish 16 3.1 Basic parameters for assessing sperm quality 3.2 New 3. tools to determine sperm quality 4. Projects, 4. grants and companies involved in this Thesis 17 18 23 OBJECTIVES 25 CHAPTER 1 Standardization of European eel (Anguilla anguilla) sperm motility evaluation by CASA software 29 CHAPTER 2 Study of the effects of thermal regime and alternative hormonal treatments on the reproductive performance of European eel males (Anguilla anguilla) during induced sexual maturation 47
CHAPTER 3 Intracellular changes in Ca 2+ , K + and pH after sperm motility activation in the European eel (Anguilla anguilla) 71 CHAPTER 4 Study of pufferfish (Takifugu niphobles) sperm: development of methods for short-term storage, effects of different activation media and role of intracellular changes in Ca 2+ and K + in the initiation of sperm motility 83 CHAPTER 5 Relationship between spermatozoa motility parameters, sperm/egg ratio, and fertilization and hatching rates in pufferfish (Takifugu niphobles) 105 GENERAL DISCUSSION 123 1. Improvements to the control of European eel reproduction 2. Sperm quality biomarkers: which, how and where to apply? 3. New data about the sperm activation process in marine fish 125 128 130 CONCLUSIONS 133 REFERENCES 137
Page 2 and 3: SPERM PHYSIOLOGY AND QUALITY IN TWO
Page 4 and 5: ACKNOWLEDGEMENTS Muchos años han p
Page 6 and 7: aunque tiene una alcachofa en la ca
Page 10 and 11: SUMMARY SUMMARY The conservation st
Page 12 and 13: RESUMEN RESUMEN Las especies objeto
Page 14 and 15: RESUM RESUM Les espècies objecte d
Page 16: GENERAL INTRODUCTION 7
Page 19 and 20: GENERAL INTRODUCTION who postulated
Page 21 and 22: GENERAL INTRODUCTION 2. Sperm physi
Page 23 and 24: GENERAL INTRODUCTION Finally, the a
Page 25 and 26: GENERAL INTRODUCTION 3. Sperm quali
Page 27 and 28: GENERAL INTRODUCTION in fish (Kime
Page 29 and 30: GENERAL INTRODUCTION However, for s
Page 31 and 32: GENERAL INTRODUCTION future applica
Page 33 and 34: GENERAL INTRODUCTION With regards t
Page 36: OBJECTIVES The chapters presented b
Page 40 and 41: STANDARDIZATION OF SPERM MOTILITY E
Page 56: CHAPTER 2 Study of the effects of t
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CHAPTER 2 reducing the pressure on
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CHAPTER 2 ongoing task in order to
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CHAPTER 2 To measure sperm density,
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CHAPTER 2 Spermiating males (%) 100
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CHAPTER 2 100 80 60 I II III IV A 4
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CHAPTER 2 3.2 Hormonal treatments P
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CHAPTER 2 With regard to the sperm
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CHAPTER 2 Relative volume (%) 80 I
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CHAPTER 2 In this respect, it is we
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CHAPTER 2 treatment to that of the
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CHAPTER 2 Our results demonstrate t
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ROLE OF IONS IN THE SPERM ACTIVATIO
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CHAPTER 4 Study of pufferfish (Taki
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CHAPTER 4 2002) so Takifugu niphobl
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CHAPTER 4 carrying out sperm collec
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CHAPTER 4 Table 1. Activation media
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CHAPTER 4 TM (%) 100 A a a Und-PD a
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CHAPTER 4 TM (%) 80 60 40 20 0 A a
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CHAPTER 4 TM (%) 80 A ASW100 GLU 60
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CHAPTER 4 although the activation m
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CHAPTER 4 dilution ratio and an opt
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CHAPTER 4 2006). Several studies in
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CHAPTER 4 5. Conclusions Some concl
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RELATIONSHIP BETWEEN SPERM MOTILIY
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Page 122 and 123:
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Page 128 and 129:
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GENERAL DISCUSSION 1. Improvements
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GENERAL DISCUSSION profiles induced
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GENERAL DISCUSSION more studies on
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GENERAL DISCUSSION sperm motility p
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CONCLUSIONS 133
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CONCLUSIONS vii. The coefficients o
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REFERENCES Aarestrup K, Økland F,
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REFERENCES Beirão J, Cabrita E, P
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REFERENCES Cabrita E, Robles V, Cu
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REFERENCES De Vos A, Van De Velde H
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REFERENCES Gallego V, Pérez L, Ast
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REFERENCES sperm competition and fe
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REFERENCES Liu QH, Li J, Xiao ZZ, D
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REFERENCES Mortimer ST. Effect of i
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REFERENCES Peñaranda DS, Marco-Jim
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REFERENCES Rurangwa E, Volckaert FA
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REFERENCES Tesch F. Telemetric obse
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REFERENCES Wong PYD, Lee WM, Tsang
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