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chapter 3 - RiuNet

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STANDARDIZATION OF SPERM MOTILITY EVALUATION<br />

MOT (%)<br />

b<br />

80 30 s<br />

60<br />

40<br />

60 s<br />

90 s<br />

a<br />

20<br />

a ab<br />

ab b<br />

a<br />

ab<br />

b<br />

40<br />

PM (%)<br />

30<br />

20<br />

10<br />

Fast ()<br />

60<br />

40<br />

20<br />

a ab b<br />

a<br />

ab<br />

b<br />

Medium ()<br />

5<br />

3<br />

1<br />

Slow ()<br />

30<br />

20<br />

10<br />

b ab a<br />

C-I C-II C-III<br />

Figure 3. Sperm motility parameters at different postactivation times (30, 60,<br />

and 90 seconds) on different sperm classes (C-I, C-II, and C-III). Data are<br />

expressed as mean ± SEM and different letters indicate significant differences<br />

between times. MOT, total motility; PM, progressive motility.<br />

4. Discussion<br />

The subjective sperm quality evaluation, widely used in many laboratories<br />

working with male gametes, depends on the skill, perception, and training<br />

of the researcher who evaluates the sperm samples (Kime et al. 2001,<br />

41

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