chapter 3 - RiuNet
chapter 3 - RiuNet
chapter 3 - RiuNet
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STANDARDIZATION OF SPERM MOTILITY EVALUATION<br />
MOT (%)<br />
b<br />
80 30 s<br />
60<br />
40<br />
60 s<br />
90 s<br />
a<br />
20<br />
a ab<br />
ab b<br />
a<br />
ab<br />
b<br />
40<br />
PM (%)<br />
30<br />
20<br />
10<br />
Fast ()<br />
60<br />
40<br />
20<br />
a ab b<br />
a<br />
ab<br />
b<br />
Medium ()<br />
5<br />
3<br />
1<br />
Slow ()<br />
30<br />
20<br />
10<br />
b ab a<br />
C-I C-II C-III<br />
Figure 3. Sperm motility parameters at different postactivation times (30, 60,<br />
and 90 seconds) on different sperm classes (C-I, C-II, and C-III). Data are<br />
expressed as mean ± SEM and different letters indicate significant differences<br />
between times. MOT, total motility; PM, progressive motility.<br />
4. Discussion<br />
The subjective sperm quality evaluation, widely used in many laboratories<br />
working with male gametes, depends on the skill, perception, and training<br />
of the researcher who evaluates the sperm samples (Kime et al. 2001,<br />
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