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SPERM PHYSIOLOGY AND QUALITY IN TWO MARINE TELEOSTS: Anguilla anguilla & Takifugu niphobles Víctor Gallego Albiach This Thesis has been submitted in accordance with the requirements for the degree of Doctor at the Universitat Politècnica de València. Esta tesis ha sido presentada para optar al grado de Doctor con Mención Europea por la Universitat Politècnica de València. Valencia, 21 th October 2013
THESIS SUPERVISOR Dr. Juan F. Asturiano Nemesio Grupo de Acuicultura y Biodiversidad Instituto de Ciencia y Tecnología Animal Universitat Politècnica de València
Page 4 and 5: ACKNOWLEDGEMENTS Muchos años han p
Page 6 and 7: aunque tiene una alcachofa en la ca
Page 8 and 9: TABLE OF CONTENTS SUMMARY 1 RESUMEN
Page 10 and 11: SUMMARY SUMMARY The conservation st
Page 12 and 13: RESUMEN RESUMEN Las especies objeto
Page 14 and 15: RESUM RESUM Les espècies objecte d
Page 16: GENERAL INTRODUCTION 7
Page 19 and 20: GENERAL INTRODUCTION who postulated
Page 21 and 22: GENERAL INTRODUCTION 2. Sperm physi
Page 23 and 24: GENERAL INTRODUCTION Finally, the a
Page 25 and 26: GENERAL INTRODUCTION 3. Sperm quali
Page 27 and 28: GENERAL INTRODUCTION in fish (Kime
Page 29 and 30: GENERAL INTRODUCTION However, for s
Page 31 and 32: GENERAL INTRODUCTION future applica
Page 33 and 34: GENERAL INTRODUCTION With regards t
Page 36: OBJECTIVES The chapters presented b
Page 40 and 41: STANDARDIZATION OF SPERM MOTILITY E
Page 52 and 53:
STANDARDIZATION OF SPERM MOTILITY E
Page 54 and 55:
STANDARDIZATION OF SPERM MOTILITY E
Page 56:
CHAPTER 2 Study of the effects of t
Page 59 and 60:
CHAPTER 2 reducing the pressure on
Page 61 and 62:
CHAPTER 2 ongoing task in order to
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CHAPTER 2 To measure sperm density,
Page 65 and 66:
CHAPTER 2 Spermiating males (%) 100
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CHAPTER 2 100 80 60 I II III IV A 4
Page 69 and 70:
CHAPTER 2 3.2 Hormonal treatments P
Page 71 and 72:
CHAPTER 2 With regard to the sperm
Page 73 and 74:
CHAPTER 2 Relative volume (%) 80 I
Page 75 and 76:
CHAPTER 2 In this respect, it is we
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CHAPTER 2 treatment to that of the
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CHAPTER 2 Our results demonstrate t
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ROLE OF IONS IN THE SPERM ACTIVATIO
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Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92:
CHAPTER 4 Study of pufferfish (Taki
Page 95 and 96:
CHAPTER 4 2002) so Takifugu niphobl
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CHAPTER 4 carrying out sperm collec
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CHAPTER 4 Table 1. Activation media
Page 101 and 102:
CHAPTER 4 TM (%) 100 A a a Und-PD a
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CHAPTER 4 TM (%) 80 60 40 20 0 A a
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CHAPTER 4 TM (%) 80 A ASW100 GLU 60
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CHAPTER 4 although the activation m
Page 109 and 110:
CHAPTER 4 dilution ratio and an opt
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CHAPTER 4 2006). Several studies in
Page 113 and 114:
CHAPTER 4 5. Conclusions Some concl
Page 116 and 117:
RELATIONSHIP BETWEEN SPERM MOTILIY
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130:
Page 134 and 135:
GENERAL DISCUSSION 1. Improvements
Page 136 and 137:
GENERAL DISCUSSION profiles induced
Page 138 and 139:
GENERAL DISCUSSION more studies on
Page 140 and 141:
GENERAL DISCUSSION sperm motility p
Page 142:
CONCLUSIONS 133
Page 145 and 146:
CONCLUSIONS vii. The coefficients o
Page 148 and 149:
REFERENCES Aarestrup K, Økland F,
Page 150 and 151:
REFERENCES Beirão J, Cabrita E, P
Page 152 and 153:
REFERENCES Cabrita E, Robles V, Cu
Page 154 and 155:
REFERENCES De Vos A, Van De Velde H
Page 156 and 157:
REFERENCES Gallego V, Pérez L, Ast
Page 158 and 159:
REFERENCES sperm competition and fe
Page 160 and 161:
REFERENCES Liu QH, Li J, Xiao ZZ, D
Page 162 and 163:
REFERENCES Mortimer ST. Effect of i
Page 164 and 165:
REFERENCES Peñaranda DS, Marco-Jim
Page 166 and 167:
REFERENCES Rurangwa E, Volckaert FA
Page 168 and 169:
REFERENCES Tesch F. Telemetric obse
Page 170 and 171:
REFERENCES Wong PYD, Lee WM, Tsang
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