INVESTIGATIONS INTO HYPERLIPIDEMIA AND ITS POSSIBLE ...

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Blood collection and handling
Owners living in relative proximity to the Gastrointestinal Laboratory at Texas
A&M University were instructed to schedule an appointment for blood collection at that
location. Owners that could not come to Texas A&M for blood collection were each sent
a styrofoam box containing ice packs and the material necessary for blood collection,
and were asked to schedule an appointment with their veterinarian for blood collection.
Ten milliliters of blood were collected from each dog into a red-top tube (with no
additive). Immediately after clot formation, the samples were centrifuged and the serum
was separated from the clot. Samples not collected at Texas A&M University were sent
to the Gastrointestinal Laboratory packed on ice by overnight courier. Serum samples
were stored at -80 o C until further use.
Questionnaires and consent forms
Owners of all dogs were asked to complete a questionnaire for each dog.
Questions covered date of birth, sex, body weight, current diet(s), current medications,
and current and past health history of the dogs. Questionnaires from all dogs were
reviewed to determine whether the dogs fit the inclusion criteria for each group. All
owners signed an informed owner consent form. The study protocol was reviewed and
approved by the Clinical Research Review Committee at Texas A&M University.