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UNIVERSITI PUTRA MALAYSIA<br />
<strong>MORPHOLOGY</strong> <strong>AND</strong> <strong>GENERAL</strong> <strong>REPRODUCTIVE</strong> <strong>STAGES</strong> <strong>OF</strong><br />
Pangasius nasutus FROM SG. PAHANG IN MARAN DISTRICT,<br />
PAHANG, MALAYSIA<br />
MOHD ZAFRI BIN HASSAN<br />
FP 2006 17
<strong>MORPHOLOGY</strong> <strong>AND</strong> <strong>GENERAL</strong> <strong>REPRODUCTIVE</strong> <strong>STAGES</strong> <strong>OF</strong> Pangasius<br />
nasutus FROM SG. PAHANG IN MARAN DISTRICT, PAHANG, MALAYSIA<br />
MOHD ZAFRI BIN HASSAN<br />
MASTER <strong>OF</strong> SCIENCE<br />
UNIVERSITI PUTRA MALAYSIA<br />
2006
<strong>MORPHOLOGY</strong> <strong>AND</strong> <strong>GENERAL</strong> <strong>REPRODUCTIVE</strong> <strong>STAGES</strong> <strong>OF</strong> Pangasius<br />
nasutus FROM SG. PAHANG IN MARAN DISTRICT, PAHANG, MALAYSIA<br />
By<br />
MOHD ZAFRI BIN HASSAN<br />
Thesis Submitted to the School of Graduate Studies, Universiti Putra<br />
Malaysia in Fulfilment of Requirement for the Degree of Master of Science<br />
November 2006
Specially dedicated to my beloved parents<br />
ii
Abstract of thesis presented to the Senate of Universiti Putra Malaysia in<br />
fulfilment of the requirement for the Degree of Master of Science<br />
<strong>MORPHOLOGY</strong> <strong>AND</strong> <strong>GENERAL</strong> <strong>REPRODUCTIVE</strong> <strong>STAGES</strong> <strong>OF</strong> Pangasius<br />
nasutus FROM SG. PAHANG IN MARAN DISTRICT, PAHANG, MALAYSIA<br />
Chairman : Annie Christianus, PhD<br />
By<br />
MOHD ZAFRI BIN HASSAN<br />
June 2006<br />
Faculty<br />
: Agriculture<br />
The present study was conducted to describe the morphological differences<br />
between P. nasutus and P. conchophilus, to describe and classify the histology<br />
of oocyte and spermatogenic cells of mature P. nasutus and to observe the<br />
seasonal patterns of the gonadal stages in twenty specimens of P. nasutus and<br />
P. conchophilus from Maran. In order to signify the species differences, twenty<br />
specimens of Thai-origin P. conchophilus from a cage culture at Sungai Pahang<br />
near Pekan, Pahang, Malaysia were also examined. The morphometric<br />
measurements were presented in percentage of standard length (%SL),<br />
predorsal length (%PDL) and head length (%HL). Based on the classification<br />
results, all P. nasutus specimens were different from P. conchophilus in both<br />
populations, in terms of snout length, eye diameter, lower and upper jaw length.<br />
A total of 205 P. nasutus were caught during the study period, from October 2004<br />
to September 2005 for the gonadal stages study of P. nasutus. Nine distinctive<br />
iii
oocytes stages observed in the study were from oogonia to late vitellogenesis.<br />
Six microscopic oocytes developmental stages were assigned for the studied<br />
ovaries of P. nasutus, namely resting, maturing, mature, spawning, running and<br />
spent stages. Further, microscopic observation on the ‘whole-section’ of the<br />
testes revealed that four testicular development stages could be distinguished<br />
namely early spermatogenesis, spermatogenesis, maturation and spent stages.<br />
In this study, it was found that the female and male P. nasutus were<br />
reproductively active at the beginning of rainy season which commenced in the<br />
middle quarter of 2005, from March to September coincided with the South-West<br />
monsoon. The study also revealed that P. nasutus exhibits a single-modal<br />
spawning season in a year, with multiple release of eggs during a single period.<br />
Such condition showed that P. nasutus is a multiple-spawner as supported by the<br />
group-synchronous oocyte development of the ovary. However, spawning did not<br />
commence synchronously within the population with the obvious presence of<br />
various female and male reproductive stages within a single sampling month.<br />
iv
Abstrak tesis yang dikemukakan kepada Senat Universiti Putra Malaysia sebagai<br />
memenuhi keperluan untuk Ijazah Master Sains<br />
MORFOLOGI DAN PERINGKAT PERKEMBANGAN PEMBIAKAN Pangasius<br />
nasutus DARI SG. PAHANG DI DALAM DAERAH MARAN, PAHANG,<br />
MALAYSIA<br />
Pengerusi : Annie Christianus, PhD<br />
Oleh<br />
MOHD ZAFRI BIN HASSAN<br />
Jun 2006<br />
Fakulti<br />
: Pertanian<br />
Kajian ini telah dijalankan untuk menerangkan perbezaan morfologi antara P.<br />
nasutus dan P. conchophilus, menghurai dan mengkelaskan histologi oosit dan<br />
sel-sel spermatogenik bagi P. nasutus matang dan seterusnya untuk melihat<br />
corak bermusim untuk peringkat-peringkat gonad bagi P. nasutus. Dua puluh<br />
spesimen P. nasutus dan P. conchophilus dari Maran, Pahang telah dikaji. Untuk<br />
melihat perbezaan spesies, dua puluh spesimen P. conchophius berasal dari<br />
Thailand tetapi dikultur dalam sangkar di Sungai Pahang, dekat Pekan, Pahang,<br />
Malaysia, turut dikaji. Ukuran-ukuran morfometrik diberikan dalam bentuk<br />
peratusan panjang piawai (%SL), panjang pra-dorsal (%PDL) dan panjang<br />
kepala (%HL). Berdasarkan keputusan pengkelasan, semua specimen P.<br />
nasutus adalah berbeza dari P. conchophilus pada kedua-dua populasi,<br />
terutamanya dari segi panjang muncung, diameter mata, panjang rahang bawah<br />
dan atas.<br />
v
Sejumlah 205 spesimen P. nasutus ditangkap sepanjang waktu kajian yang<br />
bermula dari Oktober 2004 sehingga September 2005. Sembilan peringkat<br />
kematangan oosit telah diperhatikan dalam kajian ini adalah dari peringkat<br />
oogonia sehingga peringkat akhir vitellogenesis. Enam peringkat perkembangan<br />
oosit yang diperhatikan secara mikroskopik untuk keseluruhan ovari P. nasutus<br />
dibahagikan kepada kepada peringkat rehat, sedang matang, matang, bersedia<br />
untuk membiak, sedang membiak dan selesai membiak. Berdasarkan<br />
pemerhatian mikroskopik bagi keseluruhan testis P. nasutus menunjukkan<br />
terdapat empat peringkat perkembangan testis yang dapat dikenalpasti iaitu,<br />
spermatogenesis awal, spermatogenesis, kematangan dan selepas pembiakan.<br />
Kajian ini mendapati, betina dan jantan P. nasutus adalah aktif membiak pada<br />
permulaan musim hujan yang bermula pada suku pertengahan tahun 2005, dari<br />
Mac sehingga September serentak dengan musim monsun tenggara. Kajian ini<br />
juga mendapati, P. nasutus mempunyai satu musim pembiakan khusus dalam<br />
satu tahun, yang dapat dilihat dengan pelepasan telur yang berperingkatperingkat<br />
pada sesuatu tempoh masa. Keadaan ini menunjukkan bahawa P.<br />
nasutus merupakan pembiak yang melepaskan telurnya secara berperingkatperingkat<br />
yang jelas dapat dilihat dari perkembangan oosit secara serentak<br />
dalam dua atau tiga kumpulan di dalam satu-satu ovari. Walaubagaimanapun,<br />
pembiakan tidak berlaku serentak di dalam populasi berdasarkan kehadiran<br />
pelbagai peringkat perkembangan reproduktif betina dan jantan semasa<br />
penyampelan di dalam satu-satu bulan.<br />
vi
ACKNOWLEDGEMENTS<br />
In the name of Allah, the Lord that I always put my belief in. Thanks to my<br />
parents and siblings for being my pillars and strength all my life.<br />
I would like to express my deepest gratitude to the chairman of my supervisory<br />
committee, Dr. Annie Christianus for her tremendous encouragement, financial<br />
assistance on the research expenses and camaraderie throughout the study<br />
period. Not forgetting, many thanks to my co-supervisors Assoc. Prof. Dr. Siti<br />
Shapor Siraj and Assoc. Prof. Dr. Siti Khalijah Daud for sharing their invaluable<br />
expertise, ideas and comments on the research conducted and write-up. Thanks<br />
to UPM for the scholarship and granting me study leave off my duty at UPM<br />
Bintulu Campus.<br />
The study could not have completed without the support and co-operations from<br />
many parties, namely Assoc. Prof. Dr. Khir Bejo and his lab assistant Saipu for<br />
allowing and helping me to use facilities in the Pathology Laboratory, Faculty of<br />
Veterinary UPM; Assoc. Prof. Dr. Mohd Salleh Kamaruddin for his permission to<br />
use Aquatic Biotechnology Laboratory, Faculty of Agriculture UPM, En. Jasni<br />
Yusof at the UPM hatchery, En. Mat Piah and his family for their friendly<br />
entertainment on the lodging and helping hands during sampling activities; and<br />
last but not least, million thanks to my all-time friends Ikram, Sazahar, Halim and<br />
Saufi to whom I owe their time and energy very much.<br />
vii
I certify that an Examination Committee has met on 28 November 2006 to<br />
conduct the final examination of Mohd Zafri Bin Hassan on his Master of Science<br />
thesis entitled “Morphology and General Reproductive Stages of Pangasius<br />
nasutus from Sg. Pahang in Maran District, Pahang, Malaysia” in accordance<br />
with Universiti Pertanian Malaysia (Higher Degree) Act 1980 and Universiti<br />
Pertanian Malaysia (Higher Degree) Regulations 1981. The committee<br />
recommends that the candidate be awarded the relevant degree. Members of the<br />
Examination Committee are as follows:<br />
Che Roos Saad, PhD<br />
Associate Professor<br />
Faculty of Agriculture<br />
Universiti Putra Malaysia<br />
(Chairman)<br />
Sharr Azni Harmin, PhD<br />
Associate Professor<br />
Faculty of Agriculture<br />
Universiti Putra Malaysia<br />
(Internal Examiner)<br />
Mustafa Kamal Abdul Satar, PhD<br />
Lecturer<br />
Faculty of Agriculture<br />
Universiti Putra Malaysia<br />
(Internal Examiner)<br />
Mazlan Abdul Ghaffar, PhD<br />
Associate Professor<br />
Faculty of Science and Technology<br />
Universiti Kebangsaan Malaysia<br />
(External Examiner)<br />
-------------------------------------------------<br />
HASANAH MOHD GHAZALI, PhD<br />
Professor/Deputy Dean<br />
School of Graduate Studies<br />
Universiti Putra Malaysia<br />
Date:<br />
viii
This thesis submitted to the Senate of Universiti Putra Malaysia and has been<br />
accepted as fulfillment of the requirement for the degree of Master of Science.<br />
The members of the Supervisory Committee are as follows:<br />
Annie Christianus, PhD<br />
Lecturer<br />
Faculty of Agriculture<br />
Universiti Putra Malaysia<br />
(Chairman)<br />
Siti Shapor Siraj, PhD<br />
Associate Professor<br />
Faculty of Science<br />
Universiti Putra Malaysia<br />
(Member)<br />
Siti Khalijah Daud, PhD<br />
Associate Professor<br />
Faculty of Science<br />
Universiti Putra Malaysia<br />
(Member)<br />
-------------------------------------<br />
AINI IDERIS, PhD<br />
Professor/Dean<br />
School of Graduate Studies<br />
Universiti Putra Malaysia<br />
Date: 8 March 2007<br />
ix
DECLARATION<br />
I hereby declare that the thesis is based on my original work except for<br />
quotations and citations which have been duly acknowledged. I also declare that<br />
it has not been previously or concurrently submitted for any other degree at UPM<br />
or other institutions.<br />
--------------------------------------<br />
MOHD ZAFRI BIN HASSAN<br />
Date: 15 February 2007<br />
x
TABLE <strong>OF</strong> CONTENTS<br />
DEDICATION<br />
ABSTRACT<br />
ABSTRAK<br />
ACKNOWLEDGEMENTS<br />
APPROVAL<br />
DECLARATION<br />
LIST <strong>OF</strong> TABLES<br />
LIST <strong>OF</strong> FIGURES<br />
LIST <strong>OF</strong> ABBREVEATIONS<br />
Page<br />
ii<br />
iii<br />
v<br />
vii<br />
viii<br />
x<br />
xiii<br />
xv<br />
xix<br />
1. INTRODUCTION<br />
1.1 Background of Patin buah in Malaysia<br />
1.2 Statement of problems<br />
1.3 Significance of the study<br />
1.4 Objectives<br />
1<br />
2<br />
3<br />
4<br />
2. LITERATURE REVIEWS<br />
2.1 Taxonomy<br />
2.2 Distribution of the family Pangasiidae<br />
2.3 Catfishes as an Economic commodity<br />
2.4 Morphological characters of the genus Pangasius nasutus<br />
2.5 The biology of catfishes<br />
2.6 Reproductive dynamics<br />
2.6.1 Spawning pattern and cycle of ovarian development in<br />
catfishes<br />
2.6.2 Testicular development of catfishes<br />
2.7 Histology of the gonadal development<br />
6<br />
9<br />
11<br />
12<br />
14<br />
15<br />
17<br />
19<br />
20<br />
3. MORPHOLOGICAL VARIATIONS BETWEEN Pangasius nasutus<br />
<strong>AND</strong> Pangasius conchophilus<br />
3.1 Introduction<br />
3.2 Materials and methods<br />
3.2.1 Study area<br />
3.2.2 Samples collection<br />
3.2.3 Sampling methods<br />
3.2.4 Morphometric and meristic characters<br />
3.2.5 Statistical analysis<br />
24<br />
25<br />
25<br />
26<br />
27<br />
31<br />
35<br />
xi
3.3 Results<br />
3.3.1 Morphological features for characterizing P. nasutus and<br />
P. conchophilus<br />
3.3.2 Meristic characters<br />
3.3.3 Morphometric characters<br />
3.4 Discussion<br />
36<br />
36<br />
39<br />
40<br />
51<br />
4. GAMETOGENESIS IN Pangasius nasutus<br />
4.1 Introduction<br />
4.2 Materials and methods<br />
4.2.1 Field sampling<br />
4.2.2 Gonads collection and macroscopic observations<br />
4.2.3 Histological preparation for gonad studies<br />
4.2.4 Oocyte and spermatogenic stages<br />
4.2.5 Fecundity estimation<br />
4.2.6 Statistical analysis<br />
4.3 Results<br />
4.3.1 Samples landed and standard length-weight relationship<br />
4.3.2 Water parameters, rainfall and water level of Pahang<br />
River near Maran<br />
4.3.3 Biological indices<br />
4.3.4 Macroscopic observation of the ovaries of P. nasutus<br />
4.3.5 Macroscopic observation of the testes of P. nasutus<br />
4.3.6 Microscopic observations on the individual stage of<br />
oocytes in P. nasutus<br />
4.3.7 Microscopic observations of the whole-section of<br />
ovarian development of P. nasutus<br />
4.3.8 Microscopic observations on the spermatogenic cells of<br />
P. nasutus<br />
4.3.9 Microscopic observations of the whole-section of<br />
testicular development of P. nasutus<br />
4.3.10 Seasonal development in the gonadal stages of P.<br />
nasutus<br />
4.3.11 Homogeneity of the ovary and fecundity of P. nasutus<br />
4.4 Discussion<br />
55<br />
56<br />
56<br />
56<br />
58<br />
63<br />
64<br />
65<br />
66<br />
66<br />
68<br />
72<br />
75<br />
77<br />
76<br />
87<br />
90<br />
92<br />
94<br />
96<br />
99<br />
5. <strong>GENERAL</strong> DISCUSSION <strong>AND</strong> CONCLUSION<br />
5.1 General discussion<br />
5.2 Conclusion<br />
REFERENCES<br />
APPENDICES<br />
BIODATA <strong>OF</strong> AUTHOR<br />
108<br />
118<br />
120<br />
133<br />
139<br />
xii
LIST <strong>OF</strong> TABLES<br />
Table<br />
Page<br />
1 Subgeneric classification of genus Pangasius 8<br />
2 The presence of genus Pangasius recorded in Malaysia 9<br />
3 Morphometric data taken were all according to Pouyaud<br />
et. al. (1999). With exception for measurements with *<br />
were after Gustiano et. al. (2004), after Lowe-McConnell<br />
(1975) and *** additional measurements in the present<br />
study<br />
4 Number of specimens with their respective meristic<br />
counts<br />
32<br />
39<br />
5 Morphometric data of P. nasutus (n = 20) 41<br />
6 Morphometric data of P. conchophilus caught from<br />
Maran (n = 20)<br />
7 Morphometric data of P. conchophilus caught from<br />
Pekan (n = 20)<br />
8 Variables that were significantly different (P < 0.05) in<br />
percentage of standard length (in % SL) (n = 20).<br />
9 Variables that were significantly different (P < 0.05) in<br />
percentage of predorsal length (in % PDL) (n = 20).<br />
10 Variables that were significantly different (P < 0.05) in<br />
percentage of head length (in % HL) (n = 20).<br />
11 Standardized canonical discriminant function coefficients<br />
for ratio percentages<br />
12 Structure matrix of canonical discriminant function<br />
coefficients for ratio percentages<br />
42<br />
43<br />
44<br />
45<br />
46<br />
48<br />
49<br />
13 Classification results based on ratio percentages 50<br />
14 Stepwise dehydration with a series of alcohol and<br />
subsequent paraffin wax infiltration<br />
59<br />
xiii
15 Step-by-step staining procedure 61<br />
16 Mean±SD biological indices of P. nasutus females<br />
caught from the Pahang River near Maran from October<br />
2004 to September 2005<br />
17 Mean±SD biological indices of P. nasutus males caught<br />
from Pahang River off Maran from October 2004 to<br />
September 2005<br />
72<br />
73<br />
18 Size range of oocyte stages of P. nasutus 78<br />
19 Estimated fecundity and frequency of spawning of P.<br />
nasutus collected from the Pahang River near Maran<br />
during the sampling period from October 2004 to<br />
September 2005<br />
98<br />
xiv
LIST <strong>OF</strong> FIGURES<br />
Figure<br />
1 Taxonomic hierarchy leading to the Family Pangasiidae<br />
(Source: Systema Naturae, 2000)<br />
Page<br />
7<br />
2 Map of study area 25<br />
3 Lateral view of the body; a) P. nasutus, b) P.<br />
conchophilus caught in Maran<br />
4 Methods used for samples collection; a) drift-net; b) longlines;<br />
c) fishing rod; and d) cage containment in the<br />
vicinity of study area<br />
5 Diagram of fishing gears used, a) drifting gillnet; b) longlines<br />
6 Diagrammatic representative of measurements taken on<br />
specimens (adapted from Pouyaud et. al. (1999) with<br />
modifications)<br />
27<br />
29<br />
30<br />
34<br />
7 Counts of gill raker of the first gill arch 35<br />
8 Lateral view of the head; a) P. nasutus, b) P.<br />
conchophilus<br />
9 Side by side comparison of dorsal view of the head; a) P.<br />
nasutus, b) P. conchophilus<br />
10 Ventral view of the head; a) P. nasutus, b) P.<br />
conchophilus<br />
11 Gutted belly showing swim bladder position located at<br />
the abdominal cavity of the body in P. nasutus; a) male,<br />
b) female<br />
12 Canonical discriminant functions plots using proportional<br />
values<br />
37<br />
37<br />
38<br />
38<br />
50<br />
13 Diagrammatic procedures of tissue histology process 62<br />
14 Length-size class of total specimens caught throughout<br />
the study period<br />
66<br />
xv
15 Log 10 -transformed length-weight relationship of P.<br />
nasutus caught from Pahang River near Maran<br />
16 Water surface temperature, dissolved oxygen (D.O) and<br />
specific conductivity (SpC) taken from the Pahang River<br />
near Maran during the sampling period from October<br />
2004 to September 2005. Mean±SD are subject to Tukey<br />
HSD in which dissimilar letters are significantly different<br />
(P
24 Nucleolar stage. Chromatin nucleolar stage; a) nucleoli in<br />
the nucleus present as chromatin form and more<br />
numbers of nucleoli are visible (100x); b) nucleoli are<br />
moving toward the nucleus periphery. Follicular layer<br />
appear as purple stained layer surrounding the oocyte<br />
(40x). Perinucleolar stage; a) Nucleoli allocated<br />
peripherally to the nucleus. Cytoplasm is stained light<br />
blue (20x); b) Dark spot granules can be observed<br />
alongside the follicular layer. A sign of the formation of<br />
granulosa layer (40x)<br />
25 Cortical alveoli stage, a) Early cortical alveoli stage with<br />
translucent dots at the peripheral oocyte indicating the<br />
presence of yolk vesicles (40x); b) Follicular envelope<br />
showing the developing vitelline membrane (100x); c)<br />
More ‘round’ yolk vesicles are surrounding the ooplasm<br />
periphery (20x); d) The prominent yolk vesicles are a<br />
result of the coalescence of the prior yolk vesicles (100x)<br />
26 Early vitellogenic stage; a) Formation of yolk globules<br />
forming an eosin-stained ring around the nucleus (20x);<br />
b) Yolk vesicles still remained at the peripheral ooplasm<br />
(100x)<br />
27 Vitellogenesis stage; a) Yolk globules filling up the<br />
ooplasm (5x); b) Peripheral ooplasm is filled up with<br />
smaller yolk globules, striated vitelline envelope is<br />
evident (100x)<br />
28 Late vitellogenesis stage; a) oil droplets present in the<br />
ooplasm and the start of germinal vesicle breakdown<br />
(10x); b) germinal vesicle leaves the nucleus and moves<br />
towards the animal pole at the peripheral ooplasm and<br />
yolk begins to coalesce (10x)<br />
29 Atretic oocytes; a-b) atresia during cortical alveoli (20x);<br />
c-d) atresia during vitellogenesis (40x); e-f) final stage of<br />
atresia (100x and 20x, respectively)<br />
30 Resting ovarian stage (from a sample of 1600g BW,<br />
43cm SL and 0.25 GSI)<br />
31 Ovarian development at GSI ≤ 1.0; a) Progressing -<br />
maturing (from a sample of 910g BW, 45cm SL and 0.14<br />
GSI); b) Regressing - spent (from a sample of 710g BW,<br />
43cm SL and 0.44 GSI).<br />
81<br />
82<br />
83<br />
84<br />
85<br />
86<br />
87<br />
88<br />
xvii
32 Ovarian development at GSI 1.1 – 5.0; a) Progressing -<br />
mature (from a sample of 1050g BW, 41cm SL and 2.02<br />
GSI); b) Regressing - running (from a sample of 700g<br />
BW, 36cm SL and 2.38 GSI).<br />
33 Ovarian development at GSI ≥ 5.1. Spawning (from a<br />
sample of 1300g BW, 45cm SL and 7.95 GSI).<br />
34 Developmental stages of spermatogenic cells; a)<br />
spermatogonia; b) spermatocytes; c) spermatids;<br />
appearance is blotchy and in clutch form; spermatozoa;<br />
mature spermatozoa have distinct basophilic spherical<br />
heads<br />
35 Testicular development of P. nasutus; a) Early<br />
spermatogenic stage; (from a sample of 45cm SL, 930g<br />
BW and 0.14 GSI); b) Spermatogenic stage; (from a<br />
sample of 54cm SL, 1450g BW and 0.85 GSI); c)<br />
Maturation stage; (from a sample of 41cm SL, 780g BW<br />
and 3.35 GSI); d) Spent stage; (from a sample of 44cm<br />
SL, 770g BW and 1.06 GSI).<br />
36 Frequency of occurrences of ovarian development of the<br />
females and males of P. nasutus caught from the<br />
Pahang River near Maran from October 2004 to<br />
September 2005. Number in parenthesis ( ) represents<br />
the number of specimens caught<br />
37 Frequency of occurrences of ovarian development of the<br />
females and males of P. nasutus caught from the<br />
Pahang River near Maran from October 2004 to<br />
September 2005. Number in parenthesis ( ) represents<br />
the number of specimens caught<br />
89<br />
90<br />
91<br />
93<br />
95<br />
96<br />
xviii
LIST <strong>OF</strong> ABBREVIATIONS<br />
Analysis of Variance<br />
Body weight<br />
ANOVA<br />
BW<br />
Celsius degree °C<br />
Centimeter<br />
Department<br />
Fatsomatic index<br />
Gonadosomatic index<br />
Gut repletion index<br />
Gram<br />
Head length<br />
Hepatosomatic index<br />
Kilogram<br />
Meter<br />
Milimeter<br />
cm<br />
Dept.<br />
FSI<br />
GSI<br />
GRI<br />
g<br />
HL<br />
HSI<br />
kg<br />
m<br />
mm<br />
Micrometer µm<br />
Percent %<br />
Predorsal length<br />
Standard deviation<br />
Standard length<br />
Universiti Putra Malaysia<br />
PDL<br />
SD<br />
SL<br />
UPM<br />
xix
CHAPTER ONE<br />
INTRODUCTION<br />
1.1 Background of Patin buah in Malaysia<br />
Patin buah, a vernacular river catfish name for Pangasius nasutus in Malaysia, is<br />
one of the favourite food fish and commands a good price in the local market.<br />
Information on this native fish is lacking probably attributed to the dominant<br />
interest in the other patin, Pangasius hypopthalmus, a catfish of Thai origin<br />
introduced to Malaysia for aquaculture purpose. Popularity of the native patin<br />
buah then had been brought up by the recent awareness of its economic value<br />
and the declining of its population in the Pahang River.<br />
The occurrence and distribution of P. nasutus in Malaysia are literally not<br />
conclusive. Catfishes are known to occupy habitats ranging from upland streams<br />
to large river channels and to seasonal floodplain lagoons (Winemiller and Kelso-<br />
Winemiller, 1996). Therefore it is not a surprise that P. nasutus was known to<br />
occur in the Pahang River where the richness of the biodiversity is at its best<br />
among the rivers in Malaysia (Haslawati et al., 2004). Roberts and Vidhthayanon<br />
(1991) also reported the occurrence of this species in Chenderoh Dam, Perak.
A number of species of the family Pangasiidae are of great economical<br />
importance in Southeast Asia (Hung et al., 2004) and have high potential for<br />
aquaculture in Malaysia. At present, the wild catch of patin buah (Pangasius<br />
nasutus) and patin muncung (Helicophagus wandersii) demands as much as RM<br />
50kg -1 and RM 100kg -1 in the market, respectively. Due to its white and tender<br />
flesh, Pangasiid catfish was ranked among the top five preferred species for five<br />
consecutive years in terms of consumer preferences in Malaysia (Fisheries<br />
Annual Report, 2002).<br />
1.2 Statement of problems<br />
Recently, the decline in the abundance of patin buah in the local rivers has been<br />
reported. In fact, it is supported by the declining numbers in landing data of the<br />
species in Maran, a district along the Pahang River, which is known for patin<br />
buah (Department of Fisheries, 2004). The first successful artificial propagation<br />
however, was reported by the local Department of Fisheries in March 2004 after<br />
several years of attempt at propagating it. Despite the success, however<br />
continuous fry supply however cannot be guaranteed due to low survival rate of<br />
repeated attempt.<br />
P. conchophilus that is found in the vicinity of Pahang River was recently brought<br />
in from Thailand by the Cambodian immigrants residing in Pekan, Pahang<br />
(Haslawati et al., 2004). Due to its resemblance to P. nasutus, it was named by<br />
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the local people as ‘patin buah Kemboja’ (Cambodian patin buah). These two<br />
species are commonly mistakenly misidentified for each other due to<br />
morphological features of both species (Roberts and Vidthayanon, 1991).<br />
Although induced breeding has been successful, studies on the reproductive<br />
biology of the species sampled from its natural environment are crucial so as to<br />
gain insight and more understanding on the species. Even for fish that has been<br />
established in a farming system, the information on the reproductive aspects<br />
including the absolute and relative fecundities would be helpful to ensure a<br />
continuing success in its culture (Ali, 1993). These guidelines also constitute<br />
major approach towards establishing biodiversity management as well as<br />
breeding programme (Corriero et al., 2003; Utoh et al., 2003; Micale et al., 1996;<br />
Legendre, 1986).<br />
1.3 Significance of the study<br />
The population of P. nasutus is currently declining. In the present situation, the<br />
marketable supply patin buah is entirely dependant on the wild catch. This<br />
increases the chances for the fish to be over-exploited. Effort at culturing this<br />
species has never been attempted and the population silently decreasing.<br />
Therefore, a study on the gonadal cycle of the species is necessary as an initial<br />
step towards husbandry management.<br />
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Reproductive biology has served numerous advantages in the study of a<br />
particular species or population. Manosroi et al. (2003) stated that in order to<br />
conserve a threatened Mekong giant catfish P. gigas, maturation induction by<br />
hormone injection and seasonal endocrine regulation expressed as<br />
gonadosomatic index (GSI) are crucial. Knowledge in the reproductive biology<br />
can also lead to a better understanding and advantage in breeding a particular<br />
species before being introduced for aquaculture purposes (Hassin et al., 1997).<br />
Temporal difference in reproductive maturity at individual level allows an efficient<br />
broodstock management (Dahle et al., 2003).<br />
The study on annual cycles in the gonadal stages of wild animals will provide<br />
baseline information on body composition, which is useful in the evaluation of<br />
quality and physiological condition of animals before being cultured (Craig et al.,<br />
2000). Conservation, catch size restrictions, controlled fishing seasons or<br />
improvements of breeding technologies in hatcheries can be inferred from the<br />
study.<br />
1.4 Objectives<br />
The main objective of this study is to describe the distinctive morphological<br />
characteristics of the native P. nasutus and to differentiate these characteristics<br />
from those of the introduced P. conchophilus. Apart from that, monthly<br />
observation and assessment on the gonadal activity of P. nasutus caught from its<br />
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