il\VOLVEMENT OF RETII\OIC ACID II{ - MSpace at the University of ...

follows:
II.b. Cell Treatment
After the initial incubation period (24 hrs), cultured myocytes were treated as
CONT:
ADR:
0.1 RA+ADR:
No treatment, cells in the culture medium only
3 different concentrations (4, 8, 10pM)
0.1pM retinoic acid with 3 different concentrations of
adriamycin (4, 8, 1OpM)
1 RA+ ADR: 1.0 pM retinoic acid with 3 different concentrations
adriamycin (4, 8, 10pM)
TROL + ADR: Trolox (20¡rM) with 3 different concentrations
adriamycin (4, 8, 10pM)
of
of
0.1 RA: Retinoic Acid (0.1pM)
I RA:
Retinoic Acid (1.0 pM
ADR, group myocytes were incubated with adriamycin (4, 8 and 10¡rM) for the duration
of 8 hrs and then harvested. 0.1 RA+ADR group myocytes were pretreated with O.f ¡rfr4
retinoic acid for one hour and then incubated concomitantly with adriamycin (4, 8 and
10pM) for the next eight hours. IRA+ADR group myocytes were pretreated with 1 pM
retinoic acid for one hour and then incubated concomitantly with adriamycin (4, 8 and
10pM) for the next eight hours. Trolox group myocytes were pretreated with watersoluble
antioxidant trolox (20pM) for one hour and then incubated concomitantly with
adriamycin (4, 8 and 10pM) for the following eight hours. The 0.1 RA and lRA group
myocytes were treated with 0.1 pM retinoic acid and 1 pM retinoic acid for the period of
t hrs. Control group myocytes were kept in medium M199.
t5