il\VOLVEMENT OF RETII\OIC ACID II{ - MSpace at the University of ...

Adriamycin alone caused almost 400% increase in IOD. Trolox alone did not
cause any changes in oxidative stress level but trolox did reduce the IOD increase due to
the administration of adriamycin (Fig.28). There was a slight increase in the IOD levels
in the 0.1R4 (25%) and lRA (13%) groups when compared to the CONT groups (Fig.
28). The levels of IOD in the ADR+0.1R4 and ADR+IRA were found to be significantly
increased by l8l% and 773%o respectively, when compared to the control (Fig. 28) but
IOD was significantly decreased when compared to the ADR group (Fig. 28).
II.e. Pro and anti-apoptotic protein expression
Proapoptotic and antiapoptotic proteins were assessed by measuring the Bax and Bcl-xl
protein levels respectively in cells treated with adriamycin (ADR) , adriamycin and
trolox (TROL), adriamycin and 0.1 pM retinoic acid (ADR+0.1R4) and adriamycin and
I pM retinoic acid (ADR+IRA) using a Western Blot technique. Adriamycin was
administered to adriamycin treated cells in three separate concentrations: 4, 8 and 10 pM
for a period of 8 hrs. Exposure to other drugs was started t hr prior to adriamycin. The
results for the each group \¡/ere noÍnalized by setting the value of CONT group samples
at l00o/o. The occurrence of apoptosis in treated cells was also examined by measuring
the Bax/Bcl-xl ratio. These data are represented in Fig. 29-31.
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