novel approaches to expression and detection of oestrus in dairy cows

novel approaches to expression and detection of oestrus in dairy cows novel approaches to expression and detection of oestrus in dairy cows

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and insulin are all moderate h 2 =0.11 to 0.30, largely due to the influence diet has on these variables (Hayhurst et al., 2007). Inclusion of this data for genetic selection is relevant because of the relationship of these hormones and metabolites to NEBAL and BCS. Circulating levels of these hormones can be included in breeding values and can predict susceptibility to NEBAL and low BCS at calving, early in the animals life, which can impact upon fertility and oestrous expression (Flint et al., 2008). There has been little genetic selection for oestrous expression but several factors seem promising. Milk progesterone, GnRH response and metabolic hormone levels have all been reported to influence oestrous expression and are reported to be heritable. Inclusion of heritable estimates for indicators of strong oestrous expression could aid oestrous detection. 1.6 AIMS & OBJECTIVES Many existing methods of oestrous detection; visual, physiological and automated, have flaws in either their accuracy, efficiency or both, and do not meet the ideal requirements described by Senger (1994). Poor oestrous expression is also a hindrance to effective oestrous detection; less intense, shorter duration (Dransfield et al., 1998) and less than 50% seen in standing oestrus (Dobson et al., 2008). However, due to the multifactorial nature of the control of expression of oestrus it is difficult to identify methods to improve expression. The work described within this thesis focuses on the individual cow variation of oestrous expression. The aim is to improve expression permanently through genetics therefore to improve oestrous detection rates. Ideally 24 hour continuous automated surveillance is required to minimise labour requirements and cost. However a method that can accurately detect reliable signs of oestrus to increase detection rates from 50% to the current target of above 70% is required (DairyCo, 2009). Importantly to improve herd fertility the ideal system for identifying cows in oestrus must detect cows standing to be mounted, the definitive sign of oestrus (Orihuela, 2000) and the period which is most significantly correlated with the time of ovulation (Roelofs et al., 2005) resulting in improved conception rates. Hence there is a need to develop a robust system to identify both cows approaching oestrus and cows in oestrus (standing to be mounted), in real-time to overcome the limitations of earlier systems, in order to maximise pregnancy rates and thus profitability. 48

In summary the objective was to formulate solutions to improve oestrous detection by enhancing expression of oestrus and by developing a novel technology for precise, real-time monitoring to detect cows in oestrus. Aims of this work were to: Investigate cow factors that affect expression of oestrus measured by a current automated method of oestrous detection. Investigate individual cow factors such as genetic variation that may affect the expression of oestrus. Develop novel positioning technology to detect oestrus. The aim was to monitor 3 dimensional cows positioning to detect cows approaching oestrus and cows in oestrus. 49

<strong>and</strong> <strong>in</strong>sul<strong>in</strong> are all moderate h 2 =0.11 <strong>to</strong> 0.30, largely due <strong>to</strong> the <strong>in</strong>fluence<br />

diet has on these variables (Hayhurst et al., 2007). Inclusion <strong>of</strong> this data<br />

for genetic selection is relevant because <strong>of</strong> the relationship <strong>of</strong> these<br />

hormones <strong>and</strong> metabolites <strong>to</strong> NEBAL <strong>and</strong> BCS. Circulat<strong>in</strong>g levels <strong>of</strong> these<br />

hormones can be <strong>in</strong>cluded <strong>in</strong> breed<strong>in</strong>g values <strong>and</strong> can predict susceptibility<br />

<strong>to</strong> NEBAL <strong>and</strong> low BCS at calv<strong>in</strong>g, early <strong>in</strong> the animals life, which can<br />

impact upon fertility <strong>and</strong> oestrous <strong>expression</strong> (Fl<strong>in</strong>t et al., 2008).<br />

There has been little genetic selection for oestrous <strong>expression</strong> but several<br />

fac<strong>to</strong>rs seem promis<strong>in</strong>g. Milk progesterone, GnRH response <strong>and</strong> metabolic<br />

hormone levels have all been reported <strong>to</strong> <strong>in</strong>fluence oestrous <strong>expression</strong> <strong>and</strong><br />

are reported <strong>to</strong> be heritable. Inclusion <strong>of</strong> heritable estimates for <strong>in</strong>dica<strong>to</strong>rs<br />

<strong>of</strong> strong oestrous <strong>expression</strong> could aid oestrous <strong>detection</strong>.<br />

1.6 AIMS & OBJECTIVES<br />

Many exist<strong>in</strong>g methods <strong>of</strong> oestrous <strong>detection</strong>; visual, physiological <strong>and</strong><br />

au<strong>to</strong>mated, have flaws <strong>in</strong> either their accuracy, efficiency or both, <strong>and</strong> do<br />

not meet the ideal requirements described by Senger (1994). Poor<br />

oestrous <strong>expression</strong> is also a h<strong>in</strong>drance <strong>to</strong> effective oestrous <strong>detection</strong>; less<br />

<strong>in</strong>tense, shorter duration (Dransfield et al., 1998) <strong>and</strong> less than 50% seen<br />

<strong>in</strong> st<strong>and</strong><strong>in</strong>g <strong>oestrus</strong> (Dobson et al., 2008). However, due <strong>to</strong> the<br />

multifac<strong>to</strong>rial nature <strong>of</strong> the control <strong>of</strong> <strong>expression</strong> <strong>of</strong> <strong>oestrus</strong> it is difficult <strong>to</strong><br />

identify methods <strong>to</strong> improve <strong>expression</strong>. The work described with<strong>in</strong> this<br />

thesis focuses on the <strong>in</strong>dividual cow variation <strong>of</strong> oestrous <strong>expression</strong>. The<br />

aim is <strong>to</strong> improve <strong>expression</strong> permanently through genetics therefore <strong>to</strong><br />

improve oestrous <strong>detection</strong> rates.<br />

Ideally 24 hour cont<strong>in</strong>uous au<strong>to</strong>mated surveillance is required <strong>to</strong> m<strong>in</strong>imise<br />

labour requirements <strong>and</strong> cost. However a method that can accurately<br />

detect reliable signs <strong>of</strong> <strong>oestrus</strong> <strong>to</strong> <strong>in</strong>crease <strong>detection</strong> rates from 50% <strong>to</strong> the<br />

current target <strong>of</strong> above 70% is required (DairyCo, 2009). Importantly <strong>to</strong><br />

improve herd fertility the ideal system for identify<strong>in</strong>g <strong>cows</strong> <strong>in</strong> <strong>oestrus</strong> must<br />

detect <strong>cows</strong> st<strong>and</strong><strong>in</strong>g <strong>to</strong> be mounted, the def<strong>in</strong>itive sign <strong>of</strong> <strong>oestrus</strong><br />

(Orihuela, 2000) <strong>and</strong> the period which is most significantly correlated with<br />

the time <strong>of</strong> ovulation (Roel<strong>of</strong>s et al., 2005) result<strong>in</strong>g <strong>in</strong> improved<br />

conception rates. Hence there is a need <strong>to</strong> develop a robust system <strong>to</strong><br />

identify both <strong>cows</strong> approach<strong>in</strong>g <strong>oestrus</strong> <strong>and</strong> <strong>cows</strong> <strong>in</strong> <strong>oestrus</strong> (st<strong>and</strong><strong>in</strong>g <strong>to</strong> be<br />

mounted), <strong>in</strong> real-time <strong>to</strong> overcome the limitations <strong>of</strong> earlier systems, <strong>in</strong><br />

order <strong>to</strong> maximise pregnancy rates <strong>and</strong> thus pr<strong>of</strong>itability.<br />

48

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