novel approaches to expression and detection of oestrus in dairy cows
novel approaches to expression and detection of oestrus in dairy cows novel approaches to expression and detection of oestrus in dairy cows
and insulin are all moderate h 2 =0.11 to 0.30, largely due to the influence diet has on these variables (Hayhurst et al., 2007). Inclusion of this data for genetic selection is relevant because of the relationship of these hormones and metabolites to NEBAL and BCS. Circulating levels of these hormones can be included in breeding values and can predict susceptibility to NEBAL and low BCS at calving, early in the animals life, which can impact upon fertility and oestrous expression (Flint et al., 2008). There has been little genetic selection for oestrous expression but several factors seem promising. Milk progesterone, GnRH response and metabolic hormone levels have all been reported to influence oestrous expression and are reported to be heritable. Inclusion of heritable estimates for indicators of strong oestrous expression could aid oestrous detection. 1.6 AIMS & OBJECTIVES Many existing methods of oestrous detection; visual, physiological and automated, have flaws in either their accuracy, efficiency or both, and do not meet the ideal requirements described by Senger (1994). Poor oestrous expression is also a hindrance to effective oestrous detection; less intense, shorter duration (Dransfield et al., 1998) and less than 50% seen in standing oestrus (Dobson et al., 2008). However, due to the multifactorial nature of the control of expression of oestrus it is difficult to identify methods to improve expression. The work described within this thesis focuses on the individual cow variation of oestrous expression. The aim is to improve expression permanently through genetics therefore to improve oestrous detection rates. Ideally 24 hour continuous automated surveillance is required to minimise labour requirements and cost. However a method that can accurately detect reliable signs of oestrus to increase detection rates from 50% to the current target of above 70% is required (DairyCo, 2009). Importantly to improve herd fertility the ideal system for identifying cows in oestrus must detect cows standing to be mounted, the definitive sign of oestrus (Orihuela, 2000) and the period which is most significantly correlated with the time of ovulation (Roelofs et al., 2005) resulting in improved conception rates. Hence there is a need to develop a robust system to identify both cows approaching oestrus and cows in oestrus (standing to be mounted), in real-time to overcome the limitations of earlier systems, in order to maximise pregnancy rates and thus profitability. 48
In summary the objective was to formulate solutions to improve oestrous detection by enhancing expression of oestrus and by developing a novel technology for precise, real-time monitoring to detect cows in oestrus. Aims of this work were to: Investigate cow factors that affect expression of oestrus measured by a current automated method of oestrous detection. Investigate individual cow factors such as genetic variation that may affect the expression of oestrus. Develop novel positioning technology to detect oestrus. The aim was to monitor 3 dimensional cows positioning to detect cows approaching oestrus and cows in oestrus. 49
- Page 13 and 14: LIST OF TABLES Table 1.1 Trends in
- Page 15 and 16: LIST OF ABBREVIATIONS ˚ ˚C μM 2D
- Page 17 and 18: CHAPTER 1 - Introduction & Literatu
- Page 19 and 20: the past 50 years and duration of o
- Page 21 and 22: Oestrus growing follicle (Staigmill
- Page 23 and 24: ecomes the main inhibitor of FSH an
- Page 25 and 26: calf at 40-50 days post partum; inv
- Page 27 and 28: al., 2006). However, aged sperm hav
- Page 29 and 30: can occur within 2-3 days, but if t
- Page 31 and 32: educes the incidence of problem cow
- Page 33 and 34: oestradiol, the LH surge and ovulat
- Page 35 and 36: The secondary signs of oestrus can
- Page 37 and 38: There are also changes in normal be
- Page 39 and 40: engage in more natural behaviours i
- Page 41 and 42: 1983). Exact explanations and mecha
- Page 43 and 44: 2006) and disruption of LH secretio
- Page 45 and 46: 1.4.3.2 Milk Yield and Nutrition Di
- Page 47 and 48: influence the ability of the ovary
- Page 49 and 50: cyclicity can be delayed if dietary
- Page 51 and 52: indication for the optimal time to
- Page 53 and 54: calving), 3) pre-breeding heat date
- Page 55 and 56: 1.5.2 Physiological Changes Physiol
- Page 57 and 58: 1.5.2.3 Body and Milk Temperature T
- Page 59 and 60: physical activity and stage of the
- Page 61 and 62: caused by the general environment t
- Page 63: may be gained. This is because data
- Page 67 and 68: diet, with concentrates at milking.
- Page 69 and 70: oestrus was defined as 3 consecutiv
- Page 71 and 72: The interaction between parity and
- Page 73 and 74: individual oestrus was not signific
- Page 75 and 76: Table 2.4 The effects of the intera
- Page 77 and 78: (361 vs. 578 points, respectively,
- Page 79 and 80: oestrous expression with increasing
- Page 81 and 82: the blood (Sangsritavong et al., 20
- Page 83 and 84: CHAPTER 3 - Single Nucleotide Polym
- Page 85 and 86: population owing to previous select
- Page 87 and 88: Table 3.1 Cont. Follicle Stimulatin
- Page 89 and 90: 3.2.4 Sequencing of DNA in the Labo
- Page 91 and 92: was achieved. PCR products were rem
- Page 93 and 94: 3.4 DISCUSSION The objectives of th
- Page 95 and 96: fertility and oestrous expression.
- Page 97 and 98: CHAPTER 4 - Development of a Novel
- Page 99 and 100: In summary UWB seems a good option
- Page 101 and 102: Initial tests were carried out to i
- Page 103 and 104: Therefore this demonstrates that X
- Page 105 and 106: which is most important for achievi
- Page 107 and 108: Figure 4.9 Horizontal - Vertical Di
- Page 109 and 110: that UWB is matching the ‘truth
- Page 111 and 112: mounting cow. For example height ch
- Page 113 and 114: Backpack 1 st put on in AI stalls E
<strong>and</strong> <strong>in</strong>sul<strong>in</strong> are all moderate h 2 =0.11 <strong>to</strong> 0.30, largely due <strong>to</strong> the <strong>in</strong>fluence<br />
diet has on these variables (Hayhurst et al., 2007). Inclusion <strong>of</strong> this data<br />
for genetic selection is relevant because <strong>of</strong> the relationship <strong>of</strong> these<br />
hormones <strong>and</strong> metabolites <strong>to</strong> NEBAL <strong>and</strong> BCS. Circulat<strong>in</strong>g levels <strong>of</strong> these<br />
hormones can be <strong>in</strong>cluded <strong>in</strong> breed<strong>in</strong>g values <strong>and</strong> can predict susceptibility<br />
<strong>to</strong> NEBAL <strong>and</strong> low BCS at calv<strong>in</strong>g, early <strong>in</strong> the animals life, which can<br />
impact upon fertility <strong>and</strong> oestrous <strong>expression</strong> (Fl<strong>in</strong>t et al., 2008).<br />
There has been little genetic selection for oestrous <strong>expression</strong> but several<br />
fac<strong>to</strong>rs seem promis<strong>in</strong>g. Milk progesterone, GnRH response <strong>and</strong> metabolic<br />
hormone levels have all been reported <strong>to</strong> <strong>in</strong>fluence oestrous <strong>expression</strong> <strong>and</strong><br />
are reported <strong>to</strong> be heritable. Inclusion <strong>of</strong> heritable estimates for <strong>in</strong>dica<strong>to</strong>rs<br />
<strong>of</strong> strong oestrous <strong>expression</strong> could aid oestrous <strong>detection</strong>.<br />
1.6 AIMS & OBJECTIVES<br />
Many exist<strong>in</strong>g methods <strong>of</strong> oestrous <strong>detection</strong>; visual, physiological <strong>and</strong><br />
au<strong>to</strong>mated, have flaws <strong>in</strong> either their accuracy, efficiency or both, <strong>and</strong> do<br />
not meet the ideal requirements described by Senger (1994). Poor<br />
oestrous <strong>expression</strong> is also a h<strong>in</strong>drance <strong>to</strong> effective oestrous <strong>detection</strong>; less<br />
<strong>in</strong>tense, shorter duration (Dransfield et al., 1998) <strong>and</strong> less than 50% seen<br />
<strong>in</strong> st<strong>and</strong><strong>in</strong>g <strong>oestrus</strong> (Dobson et al., 2008). However, due <strong>to</strong> the<br />
multifac<strong>to</strong>rial nature <strong>of</strong> the control <strong>of</strong> <strong>expression</strong> <strong>of</strong> <strong>oestrus</strong> it is difficult <strong>to</strong><br />
identify methods <strong>to</strong> improve <strong>expression</strong>. The work described with<strong>in</strong> this<br />
thesis focuses on the <strong>in</strong>dividual cow variation <strong>of</strong> oestrous <strong>expression</strong>. The<br />
aim is <strong>to</strong> improve <strong>expression</strong> permanently through genetics therefore <strong>to</strong><br />
improve oestrous <strong>detection</strong> rates.<br />
Ideally 24 hour cont<strong>in</strong>uous au<strong>to</strong>mated surveillance is required <strong>to</strong> m<strong>in</strong>imise<br />
labour requirements <strong>and</strong> cost. However a method that can accurately<br />
detect reliable signs <strong>of</strong> <strong>oestrus</strong> <strong>to</strong> <strong>in</strong>crease <strong>detection</strong> rates from 50% <strong>to</strong> the<br />
current target <strong>of</strong> above 70% is required (DairyCo, 2009). Importantly <strong>to</strong><br />
improve herd fertility the ideal system for identify<strong>in</strong>g <strong>cows</strong> <strong>in</strong> <strong>oestrus</strong> must<br />
detect <strong>cows</strong> st<strong>and</strong><strong>in</strong>g <strong>to</strong> be mounted, the def<strong>in</strong>itive sign <strong>of</strong> <strong>oestrus</strong><br />
(Orihuela, 2000) <strong>and</strong> the period which is most significantly correlated with<br />
the time <strong>of</strong> ovulation (Roel<strong>of</strong>s et al., 2005) result<strong>in</strong>g <strong>in</strong> improved<br />
conception rates. Hence there is a need <strong>to</strong> develop a robust system <strong>to</strong><br />
identify both <strong>cows</strong> approach<strong>in</strong>g <strong>oestrus</strong> <strong>and</strong> <strong>cows</strong> <strong>in</strong> <strong>oestrus</strong> (st<strong>and</strong><strong>in</strong>g <strong>to</strong> be<br />
mounted), <strong>in</strong> real-time <strong>to</strong> overcome the limitations <strong>of</strong> earlier systems, <strong>in</strong><br />
order <strong>to</strong> maximise pregnancy rates <strong>and</strong> thus pr<strong>of</strong>itability.<br />
48