novel approaches to expression and detection of oestrus in dairy cows
novel approaches to expression and detection of oestrus in dairy cows novel approaches to expression and detection of oestrus in dairy cows
Figure 5.1 Figure 5.2 Figure 5.3 Figure 5.4 Figure 5.5 A diagram to show the experimental design of proof of concept trials per day 106 Graph showing mounting between 2 cows with 8 numbered points showing the sequence in time as cow 533 (blue line) stood to be mounted by cow 543 (red line) 115 Graph complementing Figure 5.2 showing only the height changes in sequence of mounting 115 Graph showing mounting between 2 cows over 14 numbered time points. This demonstrates the sequence in time as cow 533 (blue line) stood to be mounted by cow 543 (red line) 116 Graph complementing Figure 5.4, showing only the height changes in the sequence of mounting 116 Figure 5.6 Progesterone profiles from POC 1 of 12 cows 123 Figure 5.7 Progesterone profiles from POC 2 of 8 cows 126 Figure 5.8 Progesterone profiles from POC 3 of 8 cows 128 Figure 5.9 Activity graphs from POC 1, activity units plotted against date and time in 2 hourly intervals for 12 cows 130 Figure 5.10 Activity graphs from POC 2, activity units plotted against date and time in 2 hourly intervals for 8 cows 133 Figure 5.11 Activity graphs from POC 3, activity units plotted against date and time in 2 hourly intervals for 8 cows 135 xii
LIST OF TABLES Table 1.1 Trends in the UK dairy industry 1 Table 1.2 Table 2.1 Table 2.2 Table of behavioural scores relating to particular oestrous behaviour 36 Table of variables investigated for their effect on the increase in activity at oestrus 54 Effect of lactation number, parity, oestrous number and oestrous season on activity at oestrus 58 Table 2.3 Effect of milk yield on activity at oestrus 58 Table 2.4 Table 3.1 The effects of the interaction between milk yield and parity on the activity increase at oestrus 59 SNPs on genes investigated for their associations with an increase in activity at oestrus 70 Table 3.2 PCR reaction reagents 74 Table 4.1 Table 4.2 Table 5.1 Table 5.2 Table 5.3 Table 5.4 Problems and solutions concerning equipment set up for monitoring of dairy cows using UWB 98 Table showing results of 12 hour trial UWB monitoring cows in oestrus 100 Results from POC 2 showing each cow’s mounting behaviour identified from the UWB data, expressed as a percentage of the total mounts by visual observation 117 Results from POC 3 showing each cow’s mounting behaviour identified from the UWB data, expressed as a percentage of the total mounts by visual observation 117 Efficiency and accuracy of the optimised script at identifying mounts from POC 2 on day of oestrus 119 Efficiency and accuracy of the optimised script at identifying mounts from POC 3 on days of oestrus 119 Table 5.5 Number of mounts declared by the script per day in POC 2 121 xiii
- Page 1 and 2: NOVEL APPROACHES TO EXPRESSION AND
- Page 3 and 4: TABLE OF CONTENTS TABLE OF CONTENTS
- Page 5 and 6: 2.2.3 Statistical Analyses ........
- Page 7 and 8: 5.3 RESULTS .......................
- Page 9 and 10: Furthermore, automated software was
- Page 11: LIST OF FIGURES Figure 1.1 Hormonal
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- Page 17 and 18: CHAPTER 1 - Introduction & Literatu
- Page 19 and 20: the past 50 years and duration of o
- Page 21 and 22: Oestrus growing follicle (Staigmill
- Page 23 and 24: ecomes the main inhibitor of FSH an
- Page 25 and 26: calf at 40-50 days post partum; inv
- Page 27 and 28: al., 2006). However, aged sperm hav
- Page 29 and 30: can occur within 2-3 days, but if t
- Page 31 and 32: educes the incidence of problem cow
- Page 33 and 34: oestradiol, the LH surge and ovulat
- Page 35 and 36: The secondary signs of oestrus can
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- Page 39 and 40: engage in more natural behaviours i
- Page 41 and 42: 1983). Exact explanations and mecha
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- Page 45 and 46: 1.4.3.2 Milk Yield and Nutrition Di
- Page 47 and 48: influence the ability of the ovary
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LIST OF TABLES<br />
Table 1.1 Trends <strong>in</strong> the UK <strong>dairy</strong> <strong>in</strong>dustry 1<br />
Table 1.2<br />
Table 2.1<br />
Table 2.2<br />
Table <strong>of</strong> behavioural scores relat<strong>in</strong>g <strong>to</strong> particular oestrous<br />
behaviour 36<br />
Table <strong>of</strong> variables <strong>in</strong>vestigated for their effect on the <strong>in</strong>crease <strong>in</strong><br />
activity at <strong>oestrus</strong> 54<br />
Effect <strong>of</strong> lactation number, parity, oestrous number <strong>and</strong> oestrous<br />
season on activity at <strong>oestrus</strong> 58<br />
Table 2.3 Effect <strong>of</strong> milk yield on activity at <strong>oestrus</strong> 58<br />
Table 2.4<br />
Table 3.1<br />
The effects <strong>of</strong> the <strong>in</strong>teraction between milk yield <strong>and</strong> parity on the<br />
activity <strong>in</strong>crease at <strong>oestrus</strong> 59<br />
SNPs on genes <strong>in</strong>vestigated for their associations with an <strong>in</strong>crease<br />
<strong>in</strong> activity at <strong>oestrus</strong> 70<br />
Table 3.2 PCR reaction reagents 74<br />
Table 4.1<br />
Table 4.2<br />
Table 5.1<br />
Table 5.2<br />
Table 5.3<br />
Table 5.4<br />
Problems <strong>and</strong> solutions concern<strong>in</strong>g equipment set up for<br />
moni<strong>to</strong>r<strong>in</strong>g <strong>of</strong> <strong>dairy</strong> <strong>cows</strong> us<strong>in</strong>g UWB 98<br />
Table show<strong>in</strong>g results <strong>of</strong> 12 hour trial UWB moni<strong>to</strong>r<strong>in</strong>g <strong>cows</strong> <strong>in</strong><br />
<strong>oestrus</strong> 100<br />
Results from POC 2 show<strong>in</strong>g each cow’s mount<strong>in</strong>g behaviour<br />
identified from the UWB data, expressed as a percentage <strong>of</strong> the<br />
<strong>to</strong>tal mounts by visual observation 117<br />
Results from POC 3 show<strong>in</strong>g each cow’s mount<strong>in</strong>g behaviour<br />
identified from the UWB data, expressed as a percentage <strong>of</strong> the<br />
<strong>to</strong>tal mounts by visual observation 117<br />
Efficiency <strong>and</strong> accuracy <strong>of</strong> the optimised script at identify<strong>in</strong>g<br />
mounts from POC 2 on day <strong>of</strong> <strong>oestrus</strong> 119<br />
Efficiency <strong>and</strong> accuracy <strong>of</strong> the optimised script at identify<strong>in</strong>g<br />
mounts from POC 3 on days <strong>of</strong> <strong>oestrus</strong> 119<br />
Table 5.5 Number <strong>of</strong> mounts declared by the script per day <strong>in</strong> POC 2 121<br />
xiii