DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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Figure 22 - hMSCs cultured on TCP (Plastic) and PA hydrogels coated with COL-1 for 7 days after being treated with mitomycin C to inhibit proliferation. Cells on the 7kPa hydrogels and 1kPa hydrogels were isolated on 12 kPa hydrogels as mentioned in III.4. Cells were stained with anti-B-III tubulin (red), anti-Nestin antibodies (green) and <strong>DA</strong>PI (blue). This experiment was performed once (n=1). Bar corresponds to 200µm. 55
Figure 23 - hMSCs cultured on TCP (Plastic) and PA hydrogels coated with COL-1 for 7 days after being treated with mitomycin C to inhibit proliferation, the cells on the 7kPa hydrogels and 1kPa hydrogels were isolated on 12 kPa hydrogels as mentioned in III.4. Cells were stained with anti-GFAP (red), anti-O4 antibodies (green) and <strong>DA</strong>PI (blue). This experiment was performed once (n=1) for GFAP and twice for O4 (n=2). Bar corresponds to 200µm. 56
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DEPARTAMENTO DE CIÊNCIAS DA VIDA F
Page 3 and 4:
ii Queria agradecer à minha namora
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iv isolation of hUCM-MSCs was ever
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vi Conseguimos optimizar um novo pr
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viii Table of contents Acknowledgem
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x III. 6 - Influence on MSCs specif
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xii Figure 2| Proteins related to t
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xiv the change in distribution with
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xvi Cells were stained with anti-B-
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xviii List of abbreviations AA - Ac
Page 22:
1 Chapter I Introduction
Page 25 and 26: 4 There are many advantages in usin
Page 27 and 28: 6 only ones MSCs can differentiate
Page 29 and 30: Figure 1. Mechanotransduction in a
Page 31 and 32: 10 1C)(Dogterom et al., 2005). Not
Page 33 and 34: 12 extracellular ligand and the cyt
Page 35 and 36: 14 Figure 3 - The Rigidity Sensing
Page 37 and 38: 16 Figure 5 - Images depicted on A)
Page 39 and 40: 18 Figure 7 - A) Immunofluorescence
Page 41 and 42: 20 (GFAP) (is a class-III intermedi
Page 43 and 44: 22 can specify lineage towards cell
Page 45 and 46: 24 I.3 - Project rationale and expe
Page 47 and 48: 26 Figure 12 - MSCs cultured on 1 a
Page 49 and 50: 28 than that in soft substrate. Thi
Page 52: 31 Chapter II - Materials and Metho
Page 55 and 56: 34 Covance and DakoCytomation, resp
Page 57 and 58: 36 clone SAM1; mouse Pe-Cy7 anti-hu
Page 59 and 60: 38 To functionalize the complete su
Page 62: 41 Chapter III Results
Page 65 and 66: 44 2008) and also as previously est
Page 67 and 68: 12.5% Ac PBS Fold increase of hMSCs
Page 69 and 70: ≈12 kPa / COL-1 +FN ≈12 kPa / C
Page 71 and 72: 50 Figure 19 - Immunophenotype of U
Page 73 and 74: 52 coated with COL-1 (similar to wh
Page 75: 54 hydrogels of 1 and 7 kPa (for ce
Page 81 and 82: 60 Discussion MSCs are widely used
Page 83 and 84: 62 isolating MSCs on the PA hydroge
Page 85 and 86: 64 2011) and expanded on TCPs for 5
Page 88: 67 Conclusion In conclusion, we opt
Page 91 and 92: 70 Cretu, A., Castagnino, P. & Asso
Page 93 and 94: 72 McBeath, R. et al., 2004. Cell s
Page 95: 74 Wang, N., Butler, J.P., and Ingb
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DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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