DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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Figure 19 – Immunophenotype of UCM-MSCs. The Y axis is the cell density. The X axis is a logarithmic
scale of fluorescence. Cells were detached, labelled with antibodies against the indicated antigens and
analyzed by flow cytometry. Cells were negative for CD11b, HLA-DR, CD45 (red and green lines) when
compared with unlabelled MSCs (dark gray and light gray) for the 2 conditions, both from the hydrogels
and of the Plastic/TCPs (n=1).
After confirming that the cells isolated by both procedures were MSCs, we checked the
differences in terms of expression of the MSCs “stemness” markers such as CD49e,
CD73, CD13 and CD90 (Table III) and it was observed that the Mean and Median
fluorescence intensity of such markers in MSCs isolated on PA hydrogels were clearly
higher than those in MSCs isolated on TCPs. Moreover, the coefficient of variation (CV)
of the expression levels of these markers was lower in cells obtained from the PA
hydrogel when compared to cells obtained from TCPs, suggesting that the population
of cells isolated and expanded on the PA hydrogels might be more homogeneous.