DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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control, we used cells from the same biological sample, whose fragments had been
plated on TCPs and the resulting cells also cultured on plastic until passage 2. The cells
were cultured until subconfluency, detached with accutase and labelled with
antibodies against cell surface markers typically used for the characterization of MSCs
and analyzed by flow cytometry (Figure 18 and 19). Flow cytometry analysis showed
that the cells were strongly positive for CD49e, CD13, CD73 and CD90. In contrast, the
cells did not expresse CD45 (hematopoietic lineage marker), CD11b and HLA-DR. This
analysis showed that the MSCs obtained using the two isolation procedures (on TCPs
or on the PA hydrogels) have similar phenotypic profiles, consistent with an MSC
phenotype (Dominici et al., 2006).
Figure 18 - Immunophenotype of UCM-MSCs. The Y axis is the cell density. The X axis is a logarithmic
scale of fluorescence. Cells were detached, labelled with antibodies against the indicated antigens and
analyzed by flow cytometry. Cells were positive for CD49e, CD73, CD13 and CD90 (red and green lines)
when compared with unlabeled MSCs (dark gray and light gray) for the 2 conditions, both from the PA
hydrogels and the Plastic/TCPs (n=1).