DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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31 Chapter II - Materials and Methods
Page 1 and 2: DEPARTAMENTO DE CIÊNCIAS DA VIDA F
Page 3 and 4: ii Queria agradecer à minha namora
Page 5 and 6: iv isolation of hUCM-MSCs was ever
Page 7 and 8: vi Conseguimos optimizar um novo pr
Page 9 and 10: viii Table of contents Acknowledgem
Page 11 and 12: x III. 6 - Influence on MSCs specif
Page 13 and 14: xii Figure 2| Proteins related to t
Page 15 and 16: xiv the change in distribution with
Page 17 and 18: xvi Cells were stained with anti-B-
Page 19 and 20: xviii List of abbreviations AA - Ac
Page 22: 1 Chapter I Introduction
Page 25 and 26: 4 There are many advantages in usin
Page 27 and 28: 6 only ones MSCs can differentiate
Page 29 and 30: Figure 1. Mechanotransduction in a
Page 31 and 32: 10 1C)(Dogterom et al., 2005). Not
Page 33 and 34: 12 extracellular ligand and the cyt
Page 35 and 36: 14 Figure 3 - The Rigidity Sensing
Page 37 and 38: 16 Figure 5 - Images depicted on A)
Page 39 and 40: 18 Figure 7 - A) Immunofluorescence
Page 41 and 42: 20 (GFAP) (is a class-III intermedi
Page 43 and 44: 22 can specify lineage towards cell
Page 45 and 46: 24 I.3 - Project rationale and expe
Page 47 and 48: 26 Figure 12 - MSCs cultured on 1 a
Page 49 and 50: 28 than that in soft substrate. Thi
Page 54 and 55: 33 II - Materials and methods II.1
Page 56 and 57: 35 al., 2009). Then, the umbilical
Page 58 and 59: 37 Table I - Composition of the hyd
Page 60: 39 Frequency sweeps were performed
Page 64 and 65: 43 III - Results III.1 - Rheologica
Page 66 and 67: 45 Figure 15 - Representative image
Page 68 and 69: 47 III.4 - Isolation and Proliferat
Page 70 and 71: 49 control, we used cells from the
Page 72 and 73: 51 Table III: Mean, Median and Coef
Page 74 and 75: 53 Figure 21- hMSCs cultured on TCP
Page 76 and 77: Figure 22 - hMSCs cultured on TCP (
Page 79: 58 Chapter IV - Discussion and Conc
Page 82 and 83: 61 The mechanical aspects of the hy
Page 84 and 85: 63 may speculate that FN provides c
Page 86: 65 may have caused the cells to los
Page 90 and 91: 69 III. References Allingham, J.S.,
Page 92 and 93: 71 Eyckmans, J. et al., 2011. A hit
Page 94 and 95: 73 Sanchez-Ramos, J., S. Song, et a

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