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Tsuji et. al. amniotic membrane-derived stem cell (2009/205260-R3 ...

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<strong>Tsuji</strong> <strong>et</strong>. <strong>al</strong>. <strong>amniotic</strong> <strong>membrane</strong>-<strong>derived</strong> <strong>stem</strong> <strong>cell</strong><br />

(<strong>2009</strong>/<strong>205260</strong>-<strong>R3</strong>)<br />

regulatory T <strong>cell</strong>s in the Wistar rat heart.<br />

Unmerged images of Fig 5 E are shown. Please see d<strong>et</strong>ail in the legend of<br />

Fig. 5.<br />

Online Fig X. In vitro transdifferentiation ability of hAMCs to the other organs.<br />

Laser confoc<strong>al</strong> microscopic view of immunocytochemistry. Hepatogenic<br />

differentiation (A,D), pancreogenesis (B), osteogenesis (C), and neurogenesis<br />

(E,F) were examined (See <strong>al</strong>so supplement<strong>al</strong> Materi<strong>al</strong> and M<strong>et</strong>hod 3).<br />

Transdifferentiated hAMCs expressed organ specific antigens, however, failed<br />

to show organ specific morphology in vitro (A,D,E,F). Sc<strong>al</strong>e bars denote<br />

50µm.<br />

Online Fig XI. Effect of hAMCs transplantation on capillary density in vivo<br />

Immunohistochemistry using antibody against CD34 was used to d<strong>et</strong>ect<br />

capillary density. % CD34 (+) area in Non-MI area and MI area in the MIgroup<br />

and MI+hAMCs group were c<strong>al</strong>culated and averaged and shown in A.<br />

There is no difference in capillary density. Sc<strong>al</strong>e bars denote 50 µm.<br />

Online Table I. Primer s<strong>et</strong>s to d<strong>et</strong>ect cardiomyocyte-specific and associated<br />

genes<br />

Suppl 4

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