Tsuji et. al. amniotic membrane-derived stem cell (2009/205260-R3 ...

Tsuji et. al. amniotic membrane-derived stem cell
(2009/205260-R3)
transplantation, there is no significant decrease in % surviving cells. Scale
bars denote 50 µm.
Online Fig VI Massive survival of EGFP-positive hAMCs and
transdifferentiation into cardiomyocytes in Wistar rat heart.
Laser confocal microscopic view of immunohistochemistry with anti-cardiac
troponin-I antibody (Trop-I; red, C). Nuclei were stained with DAPI (blue, A).
Many EGFP-positive (EGFP; green, B) rod-shaped cells expressed Trop-I
and were survived in the Wistar rat heart even at 2 weeks after the
transplantation. Images of A-C were superimposed and shown in D. Scale in
panel A denotes 50µm.
Online Fig VII. Laser confocal microscopic view of immunohistochemistry of
transdifferentiated hAMCs in the Wistar rat heart.
Unmerged images of Fig 4 C and Fig 4 D are shown in A-F and F-J,
respectively. Please see detail in the legend of Fig. 4.
Online Fig VIII. Laser confocal microscopic view of immunohistochemistry of
transdifferentiated hAMCs in the EGFP-transgenic mouse heart.
Non EGFP-labeled hAMCs were transplanted into myocardial infarction area
of EGFP-transgenic mouse heart. Two weeks after the transplantation, laser
confocal microscopic view of immunohistochemistry with anti-!-actinin (!-
actinin; red C). Nuclei were stained with DAPI(blue, A). Many EGFP-negative
(EGFPl; green, B) rod-shaped cell expressed !-actinin were survived in the
mouse heart even at 2 weeks after the transplantation. Images of A-C were
superimposed and shown in D. White box area in D was expanded and
shown in E. Clear striation staining pattern of !-actinin was observed in
EGFP-negative hAMCs derived cells. Scale in panel C denotes 50µm.
Online Fig IX. Laser confocal microscopic view of immunohistochemistry of
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