Tsuji et. al. amniotic membrane-derived stem cell (2009/205260-R3 ...

Tsuji et. al. amniotic membrane-derived stem cell
(2009/205260-R3)
Unmerged images of Fig. 2F are shown. Please see detail in the legend of
Fig. 2. Striation pattern of differentiated hAMCs in the white box of panel D
were expanded and shown in panel F-H. Clear striation pattern of !-actinin
(red; F) is observed. !-actinin and EGFP-staining (green;G) were observed
alternately in striated manner, suggesting !-actinin is expressed in EGFP
positive cell. Scale bar denotes 20!m
Online Fig. IV. Haemodynamic parameter in myocardial infarction model of
nude rat in vivo
Measured LV parameters by echocardiogram are averaged and shown at 2
weeks and 4 weeks after the myocardial infarction (MI). (A) The left
ventricular end-diastolic dimension (LVEDd) and (B) end-systolic dimension
(LVESd) tended to improve; however, there was no statistical significance.
There was no difference in the diameter of (C) the anterior left ventricular wall
thickness (AW), and (D) posterior left ventricular wall thickness (PW).
Measured (E) left ventricular systolic pressure (LVSP), (F) left ventricular enddiastolic
pressure (LVEDP), (G) maximum positive developed left ventricular
pressure (LV dP/dt), are averaged and shown. The MI+hAMC group showed
slight improvement in LVSP and LV dP/dt. There is, however, no statistical
significance.
Online Fig V. Evidence of long term survival of EGFP-positive cardiomyocytes
in Wistar rat heart
(A) Number of injected cells, detected EGFP-positive/cardiac troponin-I
positive cardiomyocytes, and % surviving cells as a function of the number of
days after the transplantation are shown. (B-G) Representative confocal
laser microscopic view of the immunohistochemical analysis using anticardiac
troponin-I (Trop-I) antibody at the day after transplantation. Although
fluorescent intensity of EGFP was decayed as a function of the time after
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