Regenerative Medicine Brochure - Harvard Apparatus

your strategic partners in developing 

physiologically advanced technologies for 

Regenerative 

Medicine 

from Research 

to Clinical 

Applications 

Treatment, Preparation & 

Harvesting 

Engineering & Modification 

Culturing & Growth 

Physiological Assessment & 

Condition Monitoring 

Electrophysiology 

& 

Cell Biology 

Research 

Regenerative 

Medicine 

Call to receive other catalogs of interest: 

Behavioral 

Research 

Harvard 

Apparatus 

Pumps 

Electroporation 

& 

Electrofusion 

Molecular 

Sample 

Preparation

Table of Contents 

Regenerative Medicine 

2Table Harvard Apparatus | Advanced Solutions for Regenerative Medicine 

of Contents 

Introduction................................................................................................................................................................................................2-5 

Overview of Technical Documents................................................................................................................................................................6-9 

New Product Summary & Phases ................................................................................................................................................................10-11 

Table of Contents for Phases 1-4 ................................................................................................................................................................12 

Phase 1: Preparation & Harvesting Overview 

Introduction ........................................................................................................................................................................................13-14 

Drug Delivery/Infusion, Nanomite Syringe Pump ....................................................................................................................................15 

Stem Cell Cooled Injection, NanoCool Delivery System ............................................................................................................................16 

Fast Bolus Injection System ..................................................................................................................................................................17 

Pulsatile Perfusion, Pulsatile Blood Pump ..............................................................................................................................................18 

Electrospinning, Advanced Polymer Mixing Delivery System......................................................................................................................19 

Cell Culture Perfusion, PHD ULTRA Push/Pull Syringe Pump ..................................................................................................................20 

Pressure Controlled Injection Pump ........................................................................................................................................................21 

Complex Mixture/Dose Delivery, PHD ULTRA Gradient System ................................................................................................................22 

Accessories: Syringe Warmers ..............................................................................................................................................................23 

Accessories: Microfluidic Valves, Circuits, Chips & Tubing ........................................................................................................................24 

Ventilators for All Species ......................................................................................................................................................................25 

Phase 2: Engineering and Modification Overview 

Introduction ........................................................................................................................................................................................26-27 

Cellular Gene Delivery Systems ..............................................................................................................................................................27 

• Electroporation, Electrofusion, Cell Injection System, Lipsomat-Liposome Production........................................................................27 

Phase 2 Examples................................................................................................................................................................................28 

• Molecular Biology, Sample Preparation ........................................................................................................................................28 

• Protein-Drug Binding Systems ....................................................................................................................................................28 

Phase 3: Culturing & Growth Overview 

Introduction ........................................................................................................................................................................................29 

InBreath 3D Bioreactor for Hollow Organs Bronchus, Trachea & Blood Vessels ..........................................................................................30-31 

LB-2 Lung Bioreactor............................................................................................................................................................................32-33 

HPC-3, Hydrostatic Perfusion Chamber for Organ and Tissue ..................................................................................................................34 

Cell and Tissue Perfusion, Imaging & Assay Chambers ............................................................................................................................35 

Toxin and Chemical Compound Removal, FLOW-Thru Dialyzer ..................................................................................................................36 

Phase 4: Physiological Assessment & Condition Monitoring Overview 

Introduction ........................................................................................................................................................................................37-38 

Blood Gas and Ion Measurements, pH, CO 2 

, O 2 

, & Glucose Probes ..........................................................................................................39-40 

Guide for Advanced Metabolic and Physiological Evaluation of Organisms, Organs, Tissues & Cells using IR Contrast Imaging (IR-CI) ............41-43 

Visible Light Imaging, Digital Magnification System..................................................................................................................................44 

References ................................................................................................................................................................................................45-46 

Contact Us..................................................................................................................................................................................................47 

Note: For Research Use Only. Not for use in humans unless proper investigational device regulations have been followed. 

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Bioscience companies have partnered 

with leading global scientists to provide specialized 

solutions for 110 years… and we are doing it again! 

Harvard Apparatus 

and the Harvard 

Bioscience family 

of companies are 

uniquely positioned 

to develop advanced 

instrumentation to accelerate Tissue Engineering and Cell Therapy 

treatments in Regenerative Medicine. From origins in 1901, Harvard 

Bioscience Companies have worked closely with leading global 

researchers across many disciplines to produce clinical and research 

equipment with… 

– the highest performance 

– unmatched quality 

– expert support 

...necessary to meet the demands of cutting-edge life science techniques. 

In keeping with this tradition, we look forward to working with you to 

develop and supply the next generation of tools to solve the new challenges 

of Regenerative Medicine from the research lab bench to the patient. 

Harvard Apparatus Provides Equipment and Expertise 

for All Phases of Regenerative Medicine Applications 

PHASE 1 

PREPARATION 

& HARVESTING 

Surgical preparation, anesthesia 

and ventilation, surgical procedures, 

food and drug delivery, animal 

and tissue handling, processing 

and storage. 

PHASE 4 

PHYSIOLOGICAL 

ASSESSMENT & CONDITION 

MONITORING 

Validation of growth-phase and 

end-stage organ/tissue construct, 

Monitoring of organ development 

for transplant suitability. 

ANIMAL 

ORGAN- 

TISSUE-CELL 

RESEARCH 

TRANSITION 

Equipment 

& Methodologies 

HUMAN 

ORGAN- 

TISSUE-CELL 

CLINICAL 

PHASE 2 

ENGINEERING 

& MODIFICATION 

Gene delivery and modification, 

cell growth, differentiation and 

manipulation. 

PHASE 3 

CULTURING & GROWTH 

Scaffold preparation, 

decellularizaton and 

Recellularization, tissue seeding, 

scale-up and growth under 

physiological conditions. 

Introduction Harvard Apparatus | Advanced Solutions for Regenerative Medicine 

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com 3

Introduction 

Harvard Apparatus | Advanced Solutions for Regenerative Medicine 

Harvard Apparatus provides advanced 

solutions across the full range of Life Science 

& Biomedical Research Techniques Utilized in 

Regenerative Medicine 

RESEARCH: 

COMPOUND SCREENING & DRUG DEVELOPMENT 

- Protein-drug and protein-protein affinity assays 

- Infusion pumps for drug, anesthesia, or nutritional delivery 

- Ion channel and ion transport assays, cellular electrophysiology 

- Automated animal feeding stations 

- Amino acid analysis 

ORGAN FUNCTION MODELING 

- Vital sign, Blood Gas and Biopotential Monitoring and Analysis 

- Ex-vivo Physiology Workstations 

MOLECULAR SAMPLE PREPARATION 

- Desalting samples for Mass Spectroscopy analysis of cell signaling molecules 

- Isolation of nucleic acid from nuclear membranes 

- Trace enrich cell metabolic waste products for ID and eventual removal 

CELL ENGINEERING AND GENETIC MODIFICATION 

- Electroporation 

- Electrofusion 

- Pneumatic injection 

- Live cell imaging and manipulation 

MICROFLUIDICS 

- Syringe Pumps with Nanoliter Precision 

- Pulsatile Pumps for Physiological Simulation 

- Microelectromechanical systems (MEMS) 

- Lab-on-a-chip (LOC) 

- Micro Total Analysis Systems (µTAS) 

TISSUE HARVESTING AND SURGICAL PROCEDURES 

- Surgical instruments; operating tables and accessories 

SAFETY PHARMACOLOGY 

- Isolated organ & tissue perfusion 

- Controlled environments for microscope-based screening assays 

- Electrophysiological Monitoring 

- Telemetric Physiology Monitoring 

- Cardiovascular Pressure and Pressure-Volume Analysis 

REGENERATIVE MEDICINE AND TISSUE ENGINEERING 

- Lung Bioreactor for Rat and Mouse, LB-2 

- 3D Bioreactor for Trachea/Bronchus and Blood Vesselsm InBreath 

- Organ Decellularization and Matrix/Scaffold Recellularization 

- Cell Therapy Injector, NanoCool 

- Online Physiological and Metabolic Monitoring 

- Equipment for Organ Harvesting, Transport and Transplant 

- Anesthesia and Ventilation for Surgery and Recovery 

CLINICAL: 

- 3D Hollow Organ Bioreactor, InBreath 

4 


Advanced Solutions for Regenerative Medicine & Tissue Engineering & Cell Therapy 


Bioscience 

Companies 

Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 

PREPARATION & 

HARVESTING 

ENGINEERING & 

MODIFICATIONS 

CULTURING & 

GROWTH 

CONDITION 

MONITORING 

WEBSITE 


Apparatus 

Denville 

Scientific 

Hugo Sachs 

Elektronik 

Biochrom 

Warner 

Instruments 

Hoefer 

BTX 

Panlab/ 

Coulbourn 

• Identification 

Products 

• Ventilators 

• Anesthesia 

• Surgical Tools 

• Infusion Pumps 

• Spinner Flasks 

• Centrifuge 

• Pipetting Systems 

• Incubators 

• Shakers 

• Sterilizers 

• Ventilators 

• Operating Tables 

• Vital Signal Monitors 

• Fast Bolus Injections 

• Cell Delivery Systems 

• Iontophoresis 

• Liposome Prep 

• Drug Delivery Systems 

• Capillary Glass 

• Transfection Reagents 

• Ligation Kits 

• Standards 

• Visualization Kits 

• DNA Polymerase 

• PCR 

• Molecular 

Spectroscopy 

• Protein Analysis 

• ds DNA 

• Oligonucleotide 

Primers 

• DNA Melts 

• Molecular Analysis 

• Amino Acids Analysis 

• Cell Imaging 

• Molecular Analysis 

• Cell Membranes 

• Cytoplasm Toxin 

Measure 

• Cell Signaling 

Chemicals 

• Blotting 

• Electroporation 

• Electrofusion 

• In-vivo Transfection 

• Pneumatic Injection 

• 3D Bioreactors 

• Toxin & Chemical 

Removal 

• Perfusion Systems 

• Spinner Flasks 

• Centrifuges 

• Pipetting Systems 

• Incubators 

• Shakers 

• Isolated Organ Baths 

• Tissue Baths 

• 3D Bioreactors 

• Molecular 

Spectroscopy 

• Media Monitoring 

• pH Monitoring 

• Spill Sensors 

• Perfusion Chambers 

• Live Imaging 

• Blood Gas 

Measurements 

• Metabolic Monitoring 

• Data Aquisition 

and Analysis 

• Feedback & 

Control Syringes 

• Protein Measurements 

• ssDNA 

• RNA 

• Phosphorus 

• Enzyme Kinetics 

• Electrophysiology 

• Cardiopulmonary 

Mechanics 

• Data Aquisition 

and Analysis 

• Molecular 

Spectroscopy 

• Amino Acid Analyzers 

• Electrodes 

• Amplifiers 

• Perfusion Chambers 

• Imaging Chambers 

• Ion Channel 

Monitoring 

• Ussing Chambers 

• Temperature Control 

• Molecular 

Sample Preparation 

• Electrophoresis 

• Affinity 

• Ion Exchange 

• SEC/GPC 

• Filtration 

• Dialysis 

• Behavioral Apparatus: 

- Mazes 

- Rota Rods 

- Treadmills 

- Activity Monitors 

web: 

www.harvardapparatus.com 

phone: 

+1 508-893-8999 

web: 

www.denvillescientific.com 

phone: 

+1 908-757-7577 

web: 

www.hugo-sachs.de/ 

phone: 

0 76 65 - 92 00-0 

web: 

www.biochrom.co.uk/ 

phone: 

+44 (0) 1223 423723 

web: 

www.warneronline.com 

phone: 

(203) 776-0664 

web: 

www.hoeferinc.com 

phone: 

+1 508-893-8999 

web: 

www.btxonline.com 

phone: 

+1 508-893-8999 

web: 

www.panlab.com 

phone: 

34 934 750 697 

web: 

www.coulbourn.com 

phone: 

+1 610-395-3771 

Introduction Harvard Apparatus | Advanced Solutions for Regenerative Medicine 


Ask about our catalogs, 

technical guides, application 

notes and bibliographies 

Our Harvard 

We have Solutions for 

Harvard Apparatus has the largest global support network of experts, and broadest range of 

specialized products to assist you in finding the best solutions for your experimental research. 

Technical Documents 

For Animal, Organ & Cellular Physiology 

and Behavioral Research 


PHYSIOLOGY 

Equipment for virtually any Animal, Organ or Cell Biology 

Experiment from animal handling to drug infusion to 

physiological monitoring. 

BEHAVIORAL 

Fully integrated research systems covering a range of 

powerful behavioral assays. 

For Cell/Tissue Engineering, Imaging and Electrophysiology 

MICROSCOPIC LIVE CELL: 

IMAGING, INJECTION & 

ELECTROPHYSIOLOGY 

Amplifiers, imaging, 

micromanipulation and perfusion for 

the electrophysiological and 

neurological sciences. 

TRANSFECTION 

A comprehensive line of instruments 

and accessories for both 

electroporation and electrofusion of 

mammalian, bacterial, yeast, fungi, 

insect and plant cells and tissues. 

CELL MODIFICATION 

This guide explains Liposomes, 

Pneumatic Injection, Iontophoresis, 

Electroporation and Mechanical 

Injectors. 

66 


Apparatus Family: 

all Regenerative Medicine Research Applications 

Call 508-893-8999 or email regen@harvardapparatus.com for technical support or to 

request a catalog or Technical Application Guide. 

Specialized Tools for Model Organisms and Ex-Vivo Physiology 

Systems, like the IH-SR for Isolated Heart Perfusion 


MODEL ORGANISMS 

Features products used specifically for smaller organisms 

including Drosophila, Nematodes, Xenopus and Zebrafish. 

Specialized Guides for Bioresearch 

EPITHELIAL TRANSPORT 

Discusses the theory of operation and 

presents the largest collection of 

Ussing Systems available. 

CELLULAR 

ELECTROPHYSIOLOGY 

TOOLS 

Detailed guide for a range of tools for 

cell based electrophysiology assays. 

ISOLATED HEART 

Detailed system guide for the ultimate ex-vivo perfusion 

system for small rodent heart, the IH-SR. 

BILAYER WORKSTATION 

Focus on the Planar Lipid Bilayer 

Workstation as a foundation for drug 

screening as well as ion channel 

structure and function. 



Ask about our catalogs, 

technical guides, application 

notes and bibliographies 

Our Harvard 

We have Solutions for 

Harvard Apparatus has the largest global support network of experts, and broadest range of 

specialized products to assist you in finding the best solutions for your experimental research. 


Advanced Organ, Tissue and Cellular Engineering and Therapy Tools 

for Regenerative Medicine 

REGENERATIVE MEDICINE 

3D Bioreactors for Lung, Trachea/Bronchus & Blood Vessels. 

Stem Cell Injection Systems for Fast Bolus Injection, 

Pressure-Controlled Fluid Delivery, Active Cooling & Mixing of 

Cell Suspensions for Injection, Electrospinning. 


Smooth High Accuracy Flow, Very High, Very Low 

or Physiological Flow Rates 

NANO & MICROFLUIDICS, PUMPS & INFUSION 

Pulsatile Blood Pumps, Peristaltic Pumps, Syringe Pumps and full line of connectors, tubing and accessories. 

88 


Apparatus Family: 

all Regenerative Medicine Research Applications 

Call 508-893-8999 or email regen@harvardapparatus.com for technical support or to 

request a catalog or Technical Application Guide. 

Molecular Biology and Assays, Sample Preparation 

and Separations 


1D AND 2D ELECTROPHORESIS, WESTERN BLOT, IMAGING SYSTEMS, PROTEIN BINDING 

SYSTEMS, SPE CLEAN-UP, DIALYSIS CLEAN-UP, PCR REAGENTS, INCUBATORS, MIXERS 

BIOCHROM: Amino Acid Analyzers, Full Family of Spectrometers 

BTX: The In Vivo and In Vitro Experts in Electroporation and Electrofusion 

DENVILLE SCIENTIFIC: A Complete Family or PCR, Incubators and Mixers 

HOEFER: The Electrophoresis People, Molecular Sample Preparation 



New Product Summay 

NEW Systems for Regenerative 

and Tissue Engineering 

Our newly developed systems are guided by breakscientists 

and surgeons to ensure performance, 

Stem Cell Cooled Injection with high viability, 

NanoCool Delivery System (p. 16) 

Developed in the field, specifically to inject 1 ul to 100 ul of stem cells into live 

tissue while maintaining cell viability in an accurate and easy-to-use system. 

The NanoCool cools,cells to 18 degrees in the syringe during injections. 

Fast Bolus Injection System for rapid, controlled fluid delivery (p. 17) 


Now a system to deliver pressure-controlled fluid injection to intact organs and 

tissues. The system delivers rapid injections while simultaneously monitoring 

pressure at the injection site. Measured pressure modulates pump speed for 

tight control of delivered injection pulse. 

Complex Automatic Mixture/Gradient Delivery & Dosing System (p. 22) 

Flow Rate 

Stepped 

Continuous 

Time 

Ramped 

The Harvard Apparatus Mixture/Gradient systems allow you to deliver Complex 

Mixture and Dosing Protocols by controlling multiple flow streams with variable 

% concentration automatically. These serial dilutions or changes are preprogrammed 

using easy set-up Pump Methods. Injection can also be initiated 

by a triggering event from another device, allowing feedback and control loops 

to be easily implemented. For example, a pH sensor could trigger injection of 

buffer solution to a mixed drug dose in order to maintain pH at a set level. 

InBreathe Hollow Organ 3D Bioreactor, proven through 

human transplantation (p. 30-31) 

Clinically proven and published bioreactor design used in successful human 

transplantation of bronchus. Produced in conjunction with Politecnico Di Milano 

and University of Barcelona, the InBreath bioreactor is ideally suited to 

regeneration of tubular hollow organs such as trachea/bronchus, blood vessels, 

esophagus and intestines. 

10 10 


Medicine, Cell Therapy 

through research in conjunction with leading 

validity and scientific relevance. 

Dedicated Lung Bioreactor with online pulmonary monitoring (p. 32) 

Proven and published whole organ bioreactor specifically designed in 

conjunction with Massachusetts General Hospital for generation of rodent lungs. 

Real-time monitoring of respiratory mechanics and Active Ventilation during 

growth phase for organ performance validation and successful transplantation. 

New Product Summary 

Sterilizable pH, CO 2 

, O 2 

and GLUCOSE SENSORS (p. 39) 

Blood gas and metabolite measurements are critical determinants of organ and 

tissue viability and performance. These sensors are available in a range of sizes 

and configurations as well as bioreactor-integrated forms for online monitoring. 

Sterilizable and made of completely inert material, these sensors are ideal for 

bioreactor use. 

Hand-Held Microscope up to 500X Magnification (p. 59) 

Harvard Apparatus Partners Program 

Small, light weight digital microscope for Organ and Tissue preparations, 

microsurgery, injection or cannulation. Remotely placed monitor (or PC 

Connection) enables simple mounting to bioreactor-based systems. 

Harvard Apparatus is partnering with global scientific partners in academia and 

industry to license, co-develop or test device concepts that will accelerate 

regenerative medicine, cell therapy and tissue engineering in the research and 

clinical marketplace. This proven development model has consistently resulted 

in refined, successful and relevant commercial products. For development of 

advanced, specialized systems for cell, tissue, organ and in-vivo assays please 

contact us. Contact Ron Sostek 508-893-8999 x 1100, 

rsostek@harvardapparatus.com 


phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com 11 11

NEW Systems for Advancing Regenerative 

Medicine & Tissue Engineering 

Phase 1: Treatment, 

Preparation & Harvesting 

Phase 3: Culturing & Tissue 

Growth 

Phases 


1. Identification Products 

- Tattoo 

- Clips 

- RFID 

2. Hair Removal Products 

3. Surgical Equipment 

- Operating Tables & Lights 

- Magnification Products 

4. Surgical Instruments 

- Tools 

- Needles 

- Sutures 

- Catheters 

- Sterilization Equipment 

5. Anesthesia Equipment 

6. Ventilation Equipment 

7. Vital-Sign Monitoring 

- Capnographs 

- Homeothermic Blankets 

- Pulse Oximeters 

- Blood Pressure 

- EKG 

- Others 

8. Recovery Chambers 

9. Infusion Products 

- Drug Delivery 

- Anesthesia Delivery 

- Nutrient Delivery 

Phase 2: Engineering & 

Modification 

1. Cell or Tissue Modification: 

In Vivo, In Vitro, Gene Delivery 

- Pneumatic Femto Injectors 



- Iontophoresis 

- Liposomes 

- Mechanical Injectors 

2. Cell or Tissue Accessories 

- Capillary Tubes 

- Capillary Pullers 

- Capillary Forges 

- Homogenizers 

- Manipulators 

3. Perfusion Chambers 

- Imaging Culture Plates 

4. Molecular Isolation, Purification Analysis 

- Amino Acid Analysis 

- Electrophoresis 

- Western Blot 

- Protein-Ligand Interactions 

- Protein-Protein Interactions 

- DNA purification Kits 

5. Molecular Characterization 

- Spectrometers 

1. Cell & Tissue Preparation 

2. Cell Growth 

- Media Infusion Pumps 

a. physiological 

b. continuous flow 

- Imaging Chambers 

3. Cell Sorting 

- Capillary Glass 


4. Cell Harvesting 

5. 3D Organ Bioreactors 

6. Spinner Flasks 

7. Centrifuges 

8. Pipetting Systems 

9. Tissue Culture 

10. Incubators 

11. Shakers 

12. Continuous flow pumps 

Phase 4: Physiological 

Assessment & Condition 

Monitoring 

Cells 

1. Microscopic Imaging & Perfusion 

Chambers 

2. Microscopic Environmental Controls 

3. Electrophysiology Products 

4. Condition Monitors 

Tissues 

5. Isolated Tissue & Perfusion Baths 

Organs 

6. Lung Regeneration Bioreactor 

7. 3D Bioreactors – Hollow Organs 

Sensors 

8. Ion Sensors 

Ammonia, Calcium, Chloride, Ethanol, 

Glucose, Lactate, Nitrate, Nitric Oxide, 

Nitrite, Peroxide, Potassium, 

Sodium, Urea 

9. Flow 

10. Temperature 

11. Pressure 

12. Force 

13. pH, O 2 

, CO 2 

12 12 


PHASE 1: Treatment, Preparation & Harvesting 

Introduction to Phase 1 Tools, Equipment 

and Systems 

Preparation & Harvesting of Cells, 

Tissues & Organs 

1. Identification Products 

- Tattoo 

- Clips 

- RFID 

2. Hair Removal Products 

3. Surgical Equipment 

- Operating Tables & Lights 

- Magnification Products 

4. Surgical Instruments 

- Tools 

- Needles 

- Sutures 

- Catheters 

- Sterilization Equipment 

5. Anesthesia Equipment 

6. Ventilation Equipment 

7. Vital-Sign Monitoring 

- Capnographs 

- Homeothermic Blankets 

- Pulse Oximeters 

- Blood Pressure 

- EKG 

- Others 

8. Recovery Chambers 

9. Infusion Products 

- Drug Delivery 

- Anesthesia Delivery 

- Nutrient Delivery 

Phase 1: Introduction 

Phase 1 Catalog and Guides 



PHASE 1: 

Treatment, Preparation & Harvesting: 

An Experimental Example 

Phase 1: Experiments 

Prepare animal & inject drugs or stems cells 

into an organ or tissue to promote regeneration 

EXAMPLE: CARDIAC INFARCT AND STEM CELL DELIVERY APPLICATION 

EXPERIMENTAL GOAL: 

Stem cells are delivered around a cardiac infract (circled). Four to ten injections 

of volumes in the 5ul range are delivered. Each injection is delivered over a 3 

second period. 

INJECT ANESTHESIA: 

STEP 1: OPEN CHEST CAVITY 

STEP 2: INITIATE INFARCT 

STEP 3: INJECT STEM CELLS 

STEP 4: CLOSE WOUND AND RECOVERY 


Step 1 

• Hair Clipper 

• Micro Vascular 

Occluder 

• Mouse & Rat ID Tags 

• Mouse Ventilator 

• Homeothermic Blanket 

• Sugical Tools 

Step 2, 3 & 4 

• Stem Cell Delivery Pump 

• Fiber Optic Illuminator 

• Rat Ventilator 

• Hot Bead Sterilizer 

• Capnograph 

• Recovery Chamber 

• Bag Restrainers 

• Magnifying Glasses 

14 14 


NEW Nanomite for Limited Volume Drug Delivery 

Hand Held or Stereotaxic Mounted 

Superior volume delivery 

Nanomite 

Figure 1: The blue line above, clearly shows the high accuracy and smooth 

flow of the Nanomite injection compared to two optimal hand injections. 

Flow ramping injections for optimal 

spatial cell delivery 

The Nanomite does all the work. 

You need only to target the area 

of interest and step on the foot 

pedal. 

• Entire flow method recalled and performed 

• High accuracy and precision cell delivery 

system from 1.3pl to 100 µl injections with 

unmatched performance from up to a 

1 ml syringe. 

• Can be combined with our micro needles down 

to gauge 37. 

• Cells dispensed and next injection ready 

Features & Benefits 

• Light weight makes it ideal for hand-held 

stereotaxic injection 

• Easy-to-use LCD color touch screen with 

GUI interface 

• Multiple methods storage makes running 

simple or complex methods easy 

• High performance in a small package 

• Better performance than manual injections 

• Version for automatic infusing and withdrawing 

• Hand-off injections use foot-pedal activation 

Figure 2: Tissue creates backpressure but flow is gently ramped so tissue has 

a chance to expand to accommodate liquid displacement. You program a 

volume and time to be delivered, Nanomite does the rest. 

Specifications 

# of Syringes 1 

Accuracy ±0.5% 

Cable Length 6 ft (1.8 m) 

Cable Length 

Controller Dimensions 4-1/2 x 9 x 4-1/2 in (11.4 x 22.9 x 11.4 cm) (H x W x D) 

Flow Rate Maximum 

1900 µl/min 

Flow Rate Minimum 

3.3 nl/hr 

Injector Head/Actuator 7 x 1-3/8 x 2 in (17.8 x 3.5 x 5.1 x cm) (L x H x W) 

Pump Function Infusion, withdrawal, volume dispense or continuous pumping modes 

Reproducibility ±0.1% 

Syringe Diameter (Max) 6.00 mm 

Syringe Size 

Maximum 

1 ml 

Minimum 0.5 µl 

Syringe Types 

1700 , 7000 , 700 and 1000 Hamilton syringes, or any 

other syringe manufacturer with known ID in mm 

Order # Product 

70-3601 Nanomite, Infuse/Withdraw 



NEW NanoCool Injector: Fixed 18°C for Optimal 

Cell Viability or Drug Delivery 

20% increase in survivability, 76% Lower O 2 

consumption 

rate at 18 vs. 37 degrees Celcius 

16NanoCool 


16 

NanoCool Cell Delivery System 

• 18˚C syringe and eppendorf 

• Flow ramping to reduce injection site blowback 

• Programmed methods for automatic recall of 

entire cell delivery system. Up to 100 methods 

stored for recall 

• 2 year warranty. Certified with CE, UL, 

CB Scheme 

• High accuracy delivery between 1.3 pl/min 

to 68ml/min 

• Preprogrammed bolus injection mode, just 

specify injection size and time of dispense 

• Foot pedal start keeps hands free 

When injecting stem cells viability 

of cells can be a major issue. The 

Harvard Apparatus NanoCool is 

the only cooled cell injection 

system with optional 

cooled and oxygenated vortex 

mixer. The injection system and 

sample vortex holder are held at 

18°C. The cell delivery is totally 

integrated in it operation through 

the NanoCool’s microprocessor 

system: power, temperature 

injection volumes and flow 

ramping are all controlled by the 

NanoCool’s program. Keeping the 

cells at 18°C instead of 37°C 

reduces O 2 

consumption of cells 

by an average of 76%. The chart 

below shows the different organ 

utilization curves. 

NanoCool Hand-held 

or Stereotaxic 

Mounted Injector 

Temperature effect on O 2 

Consumption by Organ Cell Type 

Organ Temperature 

Oxygen Change in O 2 

Comsuption Consumption 

% Change 

Kidney 37 to 18 3.7 to 0.9 2.8 75.7 

Liver 37 to 18 3.0 to 0.6 2.4 80 

Heart 37 to 18 2.4 to 0.7 1.7 70.8 

Brain 37 to 18 1.9 to 0.4 1.5 78.9 

Muscle 37 to 18) 0.8 to 0.2 0.6 75 

Skin 37 to 18 0.4 to 0.1 0.3 75 



phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com 

Alarms 

Average Linear Force 

Syringe Diameter (Max) 

Syringe Size 

Maximum 

End-of Run alarm and over pressure alarm 

11 to 12 lbs 

6.00 mm 

1 ml 

Minimum 0.5 µl 

Syringe Types 

Foot-Pedal 

NanoCool Touch 

Screen Controller 

Figure 1: IR Temperature 

Image NanoCool at 18°C. 

1700 , 7000 ,700 and 1000 Hamilton syringes, or any 

other syringe manufacturer with known ID in mm 

Injector Head/Actuator 7 x 1-3/8 x 2 in (17.8 x 3.5 x 5.1 x cm) (L x H x W) 


70-3040 NanoCool Injector Infusion/Withdrawal 

Programmable Single Syringe

Fast Bolus Injector for Cell Therapy 

Figure 1: Bone Marrow Aspiration & infusion Needles 

Fast Bolus Injector 

Fast Bolus Injector 

• Inject a fast bolus of large or small volumes in 

a second; up to 12X faster than standard pumps 

• The highest accuracy and precision available 

• Programmable with multiple methods 

• Easy-to-use icon interface 

• CE, ETL, CSA, UL and CB scheme Global 

compliance 

• Delivery from picoliter to hundreds of Ml/min 

For delivery of fast infusion or bolus injections into Liver, Kidney or Bone 

the Fast Bolus Injector makes it easy. The Fast Bolus Injector can mount a 

40 ml syringe or larger and accurately inject 100 nl or 100ul per injection with 

the push of a foot pedal. This high linear force pump can pump up to 85 

to 433 pounds of force, easily tp inject viscous materials cell suspensions 

or pastes. 

Also a full selection of research surgical products are available: Bone 

aspiration and infusion needles, syringes, surgical equipments, 

anesthesia circuits, surgical drapes and sponges, ventilators capnographs and 

pulse oximeters, catheters, sutures, bone drill and more. 

Bolus Delivery Programming, it is as easy as setting the amount to be delivered 

and the time frame you want to deliver i.e 5ul in three seconds. 

Human Bone Marrow–Derived Mesenchymal Stem Cells for Intravascular 

Delivery of Oncolytic Adenovirus 24-RGD to Human Gliomas, [Cancer Res 

2009;69 (23):8932–40] . 

Multiple methods can be stored and recalled via touch of a button, making the 

Fast Bolus Injector the ultimate, easy to use syringe pump. Methods can be 

emailed and downloaded from your PC into the pump. Share a method with a 

colleague or duplicate a complex experimental set-up with an e-mailed method. 

Figure 2: Bone Marrow Injection Sites. 




Syringes (Min./Max.) 

Flow Rate: 

Display 

Minimum 

Maximum 

Connectors: 

0.5 µl / 140 ml 

1.56 pl/min using 0.5 µl syringe 

215.8 ml/min using 140 ml syringe 

4.3" LCD Color Display with Touchpad 

Non-Volatile Memory Stores all settings 

# Syringes/Pump 2,4,8,10 (1.4 Liters capacity) 

Linear Force (Max) 

Pusher Travel Rate: 

Minimum 

Maximum 

34 kg (75 lbs) @ 100% force selection 

0.18 µm/min 

190.80 mm/min 

Dimensions 10.16 x 30.48 x 21.59 cm (4 x 8.5 x 12 in) (H x W x D) 

Weight 

Regulatory Certifications 

4.5 kg (10 lbs) 


70-3045 Fast Bolus Injector 

CE, UL, CSA, CB Scheme, EU RoHS 

Figure 3: Catheter placed into right 

hepatic vein. 



Pulsatile Blood Pump for Generation 

of Physiological Flow 

Pulsatile Blood Pump 

Figure 1: A Novel Culture System Shows that Stem Cells Can be Grown in 3D 

and Under Physiologic Pulsatile Conditions for Tissue Engineeringof Vascular 

Grafts,Oscar Abilez, et al Journal of Surgical Research 132, 170–178 (2006) 

doi:10.1016/j.jss.2006.02.017. 

Pressure and Flow Curves Using Harvard Apparatus Model 

1421 Pulsatile Blood Pump in Isolated Perfusion of Left 

Lower Lobe of Dog Lung 


Pulastile Blood Pump 

• Pulsatile output truly simulates the ventricular 

action of the heart 

• Minimal hemolysis 

• Models for mice to large animals 

• Ideal for moving emulsions, suspensions, and 

non-Newtonian fluids such as blood 

It truly simulates the pumping action of the heart. It features silicone rubbercovered 

heart-type ball valves and smooth flow paths which minimize 

hemolysis. Only inert materials like silicone rubber, acrylic plastic, and Teflon 

contact the fluid. The pumping head is easy to take apart and reassemble and 

can be sterilized. 

Outstanding Performance 

The pulsatile output closely simulates the ventricular action of the heart. This 

action provides physiological advantages in blood flow for perfusion in 

cardiovascular and hemodynamic studies. It is ideal for isolated organ 

perfusion, whole body perfusion, blood transfers, hydration/dehydration 

procedures and blood cellular profile studies. 

Pump Mechanism 

A positive piston actuator and ball check valves provide the proportioning 

action. The product of stroke rate times stroke volume is an accurate indicator 

of the flow rate. Positive piston action prevents changes in flow rates, 

regardless of variations in resistance or back pressure. The piston always 

travels to the end of the ejection stroke, independent of the volume pumped. 

The Pump completely empties at each cycle. 

Figure 2: Pa = Pulmonary, Artery, Pressure; Pv = Pulmonary, Venous, Pressure; 

Qpa = Pulmonary Artery Blood Flow. Istrumentation: Pressure (Statham), Flow 

(Blotronex Electromagnetic Flowmeter, Recording (Electronics for Medicine. 

Note: The above data is supplied through the courtesy of Cardiorespiratory 

laboratory Columbia-Presbyterian Medical Center New York, New York, Dr. 

Alfred P. Fishman, Director. 




Syringes (Min./Max.) 

Flow Rate: 

Display 

Minimum 

Maximum 

Connectors: 

0.5 µl / 140 ml 

1.56 pl/min using 0.5 µl syringe 

220.97 ml/min using 140 ml syringe 

4.3" LCD Color Display with Touchpad 

Non-Volatile Memory Stores all settings 

# Syringes/Pump 2,4,8,10 (1.4 Liters capacity) 

Linear Force (Max) 

Pusher Travel Rate: 

Minimum 

Maximum 

34 kg (75 lbs) @ 100% force selection 

0.18 µm/min 

190.80 mm/min 

Dimensions 10.16 x 30.48 x 21.59 cm (4 x 8.5 x 12 in) (H x W x D) 

Weight 

Regulatory Certifications 

4.5 kg (10 lbs) 

CE, UL, CSA, CB Scheme, EU RoHS 

18 18 


55-3305 Pulastile Blood Pump 


Electrospinning of Novel Scaffold Fiber Materials 

Syringe Pump 

Polymer 

solution 

Pipette 

Jet 

Figure 3: Photograph of a meniscus of 

polyvinyl alcohol in aqueous solution showing 

a fibre being electrospun form a Taylor cone. 

Electrospinning 

Taylor cone 

High Voltage Supply 

Figure 1: Schematic of the Electrospinning setup. 

Collector Screen 

(Rotating or Stationary) 

Figure 4: Advanced 

Polymer Mixing 

Delivery System 

A B C D E 

Figure 2: (A) Vessel Scaffold, (B) Scaffold for Muscle, (C) Tissue Scaffold, (D) Bone Scaffold, (E) Organ Scaffolds. 

Electrospinning 

In 1934, a process was patented by Formhals, wherein an experimental design 

was outlined for the production of polymer filaments using electrostatic force. 

When used to spin less than 100 micron Fibers to micron fibers, the process is 

termed “electrospinning.” The electrospinning method uses a high voltage to 

create an electrically charged jet of polymer solution or melt, which then dries 

or solidifies to leave a polymer fiber. This is accomplished by first mounting a 

syringe into a syringe pump capable of delivering a constant pressure and 

smooth flow to a pulled spray nozzle. One electrode is placed into this spinning 

solution/melt while the other is placed onto a grounded collector, most often a 

metal wire mesh. The driving force is provided by a high voltage source 

generating up to 30kV of positive or negative polarity through the electrode 

located at the nozzle 

tip. When an electric 

field is subjected to 

the end of the nozzle 

tube a charged is 

induced on the 

surface of the liquid. 

A mutual charge 

repulsion causes a 

force directly 

opposite to the 

surface tension, such 

that as the intensity 

of the electric field is 

increased, the 

hemispherical surface of the fluid at the nozzle tip elongates into a conical 

shape known as the Taylor cone. With increasing field, a critical value is 

attained when the repulsive electrostatic force overcomes the surface tension 

and a charged jet of fluid is ejected from the tip of the Taylor cone. The jet 

undergoes a whipping process during which the solvent evaporates and the 

remaining charged polymer fiber randomly lays itself on the grounded metal 

collecting screen. By adjusting the flow of the fluid from the pump and the 

magnitude of the electric field, the spinning rate can be controlled. 

Variables: 

1. Molecular Weight, Molecular-Weight Distribution and Architecture 

2. Solution Properties: Viscosity, Conductivity, Surface Tension 

3. Electric Potential, Flow Rate, Collection Screen 

4. Distance between capillary and collection screen 

5. Ambient Parameters: temperature, humidity, chamber air velocity 

6. Motion of the Target Screen (collector) and chamber Air Velocity 

The Harvard Apparatus Family of Pumps is 

well-suited for electrospinning: 

• Regulatory compliance ensuring electrical 

currents do not damage pumps with an option 

for a custom grounding cable for added user 

protection 

• Deliver the smoothest and most accurate flows 

so that uniform fibers are created without 

droplet formation 

• Programmable flow rates with automatic time 

control; timed length of spin, optimization 

protocols can be created, powerful enough for 

viscous solutions, highest accuracy at the 

lowest (nanoliter) flow rate range 

• Rugged design with 2 year warranty and 

global support 


70-3007 PHD ULTRA Programmable Syringe Pump 



Continuous Flow for Nutrient Delivery Systems 

PHD ULTRA Push-Pull 


PHD ULTRA Push-Pull 

Programmable Syringe Pump 

• Compensating Flows 

- The control of continuous infusion and 

simultaneous withdrawal of liquids while 

monitoring fluid levels 

• Perfusion Across Tissue Beds 

- Directional control of pulseless flow across 

a tissue bed using switching valves 

• Continuous Flow with High Accuracy and 

Smooth Flow 

- Pump Any Volume Large or Small with 

Smooth, Non Pulsating Flow 

• Continuous Accurate Flow for High 

Pressure systems 

- Unlike peristaltic pumps, syringe pumps can 

pump against high pressures 

• Easily Sterilized Flow Path 

- By replacing syringe and tubing with a 

sterilized set, this pump can maintain it’s 

sterility 

Figure 1: Pressure-driven flow was continous using a programmable push-pull 

syringe pump (Harvard Apparatus, Holliston, MA). Media was equilibriated with 

10% or 21% O 2 

in a gas exchanger made with gas-permeable silastic tubing. 

The continuous flow pump can provide low pulsation, high accuracy and 

precise delivery of flow to any reactor set-up. When you are trying to provide 

low shear stress, non pulsing flow during the adhesion step of regeneration of 

cell deposition for tissue or organ development, the syringe pump provide the 

continuous Flow compensation of a peristaltic, with higher accuracies and 

precisions. These flows can be cooled or heated with in –line heaters or 

coolers, These pumps have a rugged construction which allows the to work for 

long periods of time with high reliability. These pumps come with a two year 

warranty as a testament to their durability. 


70-3009 PHD ULTRA Push-Pull Programmable Syringe Pump 

20 


Pressure Controlled Injection System 

Pressure 1 

Pressure 2 

Figure 1: Pressure Delivery Injection System 

Pressure 3 

•Directly measure pressure at site of injection 

•Simultaneously measure overall system pressure 

•Option for holding injection pressure constant 

for defined time period 

The system is specifically designed for repeatable, pressure controlled 

injections into intact organs. However a wide range of settings and accessories 

makes the system appropriate for virtuallly any semi-rigid substrate and certain 

hollow-body systems. 


Pressure-Controlled Injection System Parameters Controller 

77-0000 ML866 POWERLAB 4/304 Channel w/LABCHART Software 

to Measure System Pressure after Syringe 

72-4496 Research Grade Blood Pressure Transducer, 

115VAC/60Hz, with 6 ft cable 

Blister Pressure Measurement 

72-9845 1F SGL Pres CTH 120 cm No Rep 

Syringe Pump Capacity 2X up to 140 ml Syringes Max Flow Rate 

with 2X 140 ml Syringes is 3.68 ml/sec per Syringe or 7.36 ml/sec 

70-3007 PHD ULTRA I/W Programmable 

70-3033 PHD ULTRA Analog Central Imput 

Pressure 4 Pressure 5 

Figure 2: System components include a syringe pump, injection 

pathway, pressure measurement hardware, and data acquisition 

and feed back system. Injection parameters are programmed 

while pressure is continuously monitored. Real time pressure 

feedback modulates syringe pump to keep pressure constant. 


Constant Pressure Injection 

73-1523 PLUGSYS Minicase Type 609 

73-2806 SCP PLUGSYS Servo Control F/Perfusion with Interface 

Cable for PHD ULTRA Control 

73-0065 TAM-A HSE PLUGSYS Transducer Amplifier Module 

Type 705/1, Analog Display 

72-9843 Pressure Catheter to Tam Cable 

Accessories 

72-2673 Y-Connector FLL/FLL/MLL (Rotating), Package of 25 

72-14553-WAY FLL-FLL-FLL "T" CONNECTOR, POLYPROPYLENE, PK/25 

73-0500 Lab Stand w.Heavy Triangular Base Plate 8 mm OD Rod, 

30 cm Long. Also includes Acrylate. Block Clamp (73-0566) 

W/8 & 10 mm Opening 

72-9523 MLL to MLL Connector 

77-0156 Manual Press Cal Kit 

Pressure Delivery Injection System Harvard Apparatus | Advanced Solutions for Regenerative Medicine 


Complex Mixture/Dose Delivery System 

Mixture/Dose Delivery System 

Flow Rate 

Stepped 

Continuous 

Ramped 

Time 


Figure 1: Stepped, Continuous, and Ramped gradient. 

The new Harvard Apparatus Syringe Pump Gradient System has the ability to 

easily program step and continuous concentration gradients from two or three 

solvents (Figure 1). The Harvard Apparatus syringe pumps are highly published 

for their superior flow accuracy and precise flow performance from ul/min to 

hundreds of ml/min . Figure 2 shows the shows the flow at 1000 and 500 

ul/min. In both stem cell bolus injections and 3D reactor perfusion stream 

dosing, the Harvard Apparatus Gradient systems allows you to automatically 

deliver injections fluids or perfusion flow streams that can have variable % 

concentration automatically. These serial dilutions or solution changes are 

preprogrammed or signaled and initiated by an I/O event. In the case of 

injections you could automatically change the amount of cells in every injection 

by programming an increase in the concentration of cell solution in each 

injection, or by simply selecting a new pre-established method. In perfusions, a 

pH probe could signal for a different % composition of the perfusion stream, or 

dose a perfusion stream automatically. This could provide more buffering 

capacity or adjustment solution to the perfusion stream completely unattended. 

This new capability of Harvard Apparatus syringe pumps to completely deliver 

% composition changes automatically with high accuracy and precision flow 

provides a new economical tool to advance experiments and eliminate manual 

input for complex solution changes. 

- Enzyme studies 

- Reaction dosing solutions 

- Drug infusion experiments 

- Nutritional infusion experiments 

- Mixing polymer in electrospinning 

- Chromatography & FIA systems 

Figure 2: 1000 nl/min & 500 nl/min with 250 µl syringe Measured on a Sensirion 

Flow Sensor (PHD ULTRA) 

Figure 3: The following screen shows the set-up for a gradient. 


70-4101 PHD ULTRA Gradient System 1 Master/1 Satellite with Stand 

70-4102 PHD ULTRA Gradient System 1 Master/2 Satellites with Stand 

70-4106 PHD ULTRA Gradient System 1 Master/1 Satellite without Stand 

70-4107 PHD ULTRA Gradient System 1 Master/1 Satellite without Stand 

22 


Accessories 

Syringe and In-Line Temperature Controllers 


Heater Resistance 

Voltage Requirement 

18 Ω 

Variable to 12 V maximum 

Temperature Range Ambient to 65°C 

Temperature Accuracy 

Cable Length 

Warranty 

Syringe warmer 

mounted on a 

support stand 

Syringe warmer on 

a syringe pump 

SWS-10, SWS-60 & SWS–140 

Syringe Warmers 

• Independent temperature control for 

individual syringes 

• Designed for use on a syringe pump 

or support stand 

• Accommodates 10, 60 and 140 cc syringes 

• Scale marking ports permit volume 

monitoring during use 

• Can be powered from 12 volt battery for 

sensitive electrophysiology applications 

±1°C 

2.4 m 

One year 

Syringe Warmer Model Specifications 

Model Weight Length OD ID Syringe Type 

SWS-10 32.7 g 38.2 mm 22.2 mm 

SWS-60 76 g 83.7 mm 35.0 mm 

Order # Model Product 

16.2 mm 

Dickerson 

29.1 mm 

Dickerson 

64-1584 SWS-10 Syringe Heater for 10 cc Syringes 



64-1545 TC-124A Temperature Controller, 120 VAC US 

64-1545E TC-124AE Temperature Controller, 240 VAC Europe 

64-1655 TC-144 Temperature Controller 

64-1606 BAC-1 Battery Adapter Cable 

Becton 

Becton 

SWS-140 192 g 109.5 mm 51.0 mm 41.4 mm Monoject 

SC-20 Dual In-line Solution 

Heater/Cooler 

• Heats and cools from 0° to 50°C 

• Compatible with Warner Series 20 Chambers 

• Optimized for use with the CL-100 Bipolar 

Temperature Controller 

In-line solution heating has proven to be one of the most effective methods of 

maintaining the temperature of perfusion solutions. The SC-20 Dual In-line 

Solution Heater/Cooler utilizes Peltier thermoelectric devices to regulate 

temperature both above and below ambient levels. 

The SC-20 is designed to thermally regulate one or two solutions at the same 

temperature. Solution temperature can be maintained at 0°C at flow rates of 2 

ml/min., 5°C at 5 ml/min., or as high as 50°C at 5 ml/min. 

An integral water jacket is used to remove excess heat from the SC-20 peltier 

device. Running water either from a tap or a large reservoir can be used. Flow 

rates as low as 4 liters per hour are sufficient to maintain cooling efficiency. 

The SC-20 can be used with either one or two discrete perfusate solutions, or 

with a solution/gas combination. When coupled with a PHC Series Imaging 

Chamber Heater/Cooler Jacket, the SC-20 provides an effective means of 

temperature control in a Warner chamber, even in the absence of solution flow. 

Each SC-20 is supplied with a TA-29 Thermistor Cable Assembly for monitoring 

the bath temperature during use, 10 feet of PE-160 tubing and 10 feet of 1/8" I.D. 

x 1/4" O.D. Tygon tubing. 


Minimum Temperature 0°C (2 ml/min. max flow) 

Maximum Temperature 50°C 

Maximum Flow Rate at 5°C 5 ml/min. 

Accuracy 

±0.1°C 

Internal Dead Volume 330 µl 

Perfusion Lines 

Type 316 Stainless Steel, 0.032 in ID x 0.062 in OD 

Water Jacket Ports 

Type 316 Stainless Steel, 0.12 in ID x 0.147 in OD 

Controller 

Model CL-100 Bipolar Controller 

Physical Dimensions: 

Body (D x L) 

21 x 165 mm 

Weight 

109 g 

Cable Length 

1.9 m 

Connector Type 

15 pin Male “D” 

Warranty 

One year 

Order # Model Product 

64-0353 SC-20 Solution Heater/Cooler Two Line 

64-0352 CL-100 Bipolar Temperature Controller 

Replacement Parts 

64-0107 TA-29 Cable with Bead Thermistor for Heater Controllers 

Accessories Harvard Apparatus | Advanced Solutions for Regenerative Medicine 


Accessories 

Micro Valves & Micro Connectors 

Accessories 

Valves/Controllers 

(Fluids & Pressure) & Glass 

or Plastic Fluidic Chips 

In-Line PEEK cartridge for 

Toxic removal 


• Fast 

• Cost-effective 

• Preparation of microfluidic volumes 

Until now, commercially available options for preparing microliter volumes have 

suffered major drawbacks, including high back pressure, clogging, leakage and 

limited surface chemistry options. New CapTite cartridges offer customizable 

packing and re-use, for easy sample preparation in both prototypes and highthroughput, 

automated systems. 

Micro Fluidic Circuits & Chips 

• Compact components for 360 µm, 1/16, 

and 1/32 inch in tubing 

• Fast, breadboard-based design 

• Leak-free high pressure setups 

Routing fluids can be one of the most time-consuming tasks of microfluidics 

research. CapTite microminiature components take the pain out of 

constructing setups. Based on components developed by Sandia National 

Laboratories, these compact components for 360 µm and 1/16 in tubing are 

designed for leak-free connectivity, even at high pressures. 

Manifold/Connectors, 

Needles & Tubing 

Tubing, Connectors and Manifolds 

Harvard Apparatus has a wide range of tubing, connectors and manifolds so 

you can create the fluid pathway to meet your specific requirements. We offer 

many types of tubing of different materials and sizes. Our fluid manifolds start 

at 2 channels and go all the way up to 24 channels. We have a multitude of 

connectors that allow you to complete the fluid connections and complete your 

system. Please call our technical support staff for additional information. 

Microfluidic Construction Kit 

Qty Order # Model Product 

Single User Kit 

72-0426 C360-KIT1 Microfluidic Construction Single User Kit 

for 360 µm capillary tubing 

Kit Components: Includes Tools A, B, C, D, E (1 each) 

75 72-0431 C360-100 One-Piece Fitting 

10 72-0433 C360-101 Plug 

5 72-0438 C360-203 Tee Interconnect 

5 72-0443 C360-204 Cross Interconnect 

10 72-0449 C360-300 Luer-Lock Adapter 

25 72-0448 C360-400 Bonded Port Connector 

2 72-0445 C360-500 Selector Valve 

2 72-0430 LS-600 Breadboard 

Interface Kit 

72-0427 C360-KIT2 Microfluidic Construction Interface Kit for 

360 µm capillary tubing 

Kit Components: Includes Installation Tools A, B, D, E (1 each) 

50 72-0431 C360-100 One-Piece Fitting 

5 72-0433 C360-101 Plug 

5 72-0438 C360-203 Tee Interconnect 

5 72-0443 C360-204 Cross Interconnect 

10 72-0449 C360-300 Luer-Lock Adapter 

2 72-0445 C360-500 Selector Valve 

2 72-0430 LS-600 Breadboard 

24 


Harvard Apparatus Ventilators & Anesthesia 

The World’s Most Published Ventilation Systems for 

Stem Cell Harvesting 

Look for the complete line of products in the NEW Havard Apparatus 

Animal, Organ and Cell Physiology catalog. 

• ANESTHESIA 

• CIRCUITS 

• SCAVENGERS 

• DEWARS 

• INFUSION PUMPS 

• ANIMAL PREPARATION 

TOOLS 

• ID SYSTEMS 

• HAIR REMOVAL 

• RESTRAINERS 

• ANIMAL TEMPERATURE 

CONTROL 

• MAGNIFICATION 

PRODUCTS 

• OPERATING TABLES 

• OPERATING LIGHTS 

• RECOVERY CHAMBERS 

• SURGICAL TOOLS 

Minivent for Mice 

Inspira for Mice up to Cats 

683 for Mice & Rats 

• SUTURES AND NEEDLES 

• SCAPELS 

• FORCEP 

• STERILZERS 

• VITAL SIGN MONITORS 

• BLOOD FLOW 

• CAPNOGRAPH 

• EEG 

• GLUCOSE 

• HEART RATE 

• O 2 

• OXIMETERS 

• pH 

• PULSE RATE 

• TEMPERATURE 

Accessories Harvard Apparatus | Advanced Solutions for Regenerative Medicine 

Anesthesia Machine 


PHASE 2: Engineering & Modification 

Phase 2: Introduction 


Introduction to Phase 2 Tools, Equipment 

and Systems 

Cell, Tissue Engineering, Modification, 

Preparation & De-Cellularization 

1. Cell or Tissue Modification: 

In Vivo, In Vitro, Gene 

Delivery 

- Pneumatic Femto Injectors 



- Iontophoresis 

- Liposomes 

- Mechanical Injectors 

2. Cell or Tissue Accessories 

- Capillary Tubes 

- Capillary Pullers 

- Capillary Forges 

- Homogenizers 


PLI-100A femtoliter cell injector 

3. Perfusion Chambers 

- Imaging Culture Plates 

4. Molecular Isolation, 

Purification Analysis 

- Amino Acid Analysis 

- Electrophoresis 

- Western Blot 

- Protein-Ligand Interactions 

- Protein-Protein Interactions 

- DNA purification Kits 

5. Molecular Characterization 

- Spectrometers 


26 


PHASE 2: Engineering & Modification 

Harvard Apparatus is the Leader in Femoliter to Microliter 

Cell Injection & Transfection Products for Optimal Cell and 

Tisssue Engineering 

PLI-100A: CELL INJECTION SYSTEM 

ELECTROPORATION HIGH-THROUGHPUT 

IN VIVO, IN VITRO 

ASK FOR A 

FREE GUIDE 

Send email to: 

regen@harvardapparatus.com 

LIPOSOMAT-LIPOSOME PRODUCTION 

ELECTROFUSION & ELECTROPORATION 

Phase 2: Introduction Harvard Apparatus | Advanced Solutions for Regenerative Medicine 


PHASE 2: 

Engineering & Modification, 

Application Examples 

Phase 2: Examples 

A Range of Application Areas for Phase 2 

Tools, Equipment and Systems 


MOLECULAR BIOLOGY 

• Electrophoreses (1D,2D) 

• Molecular Biological 

Sample Prep Tools 

- Chromatographic 

- Dialysis 

- Equilibrium Dialysis 

PROTEIN-DRUG BINDING STUDIES 

• No Force Flow 

• O 2 

Pressure CO 2 

• pH Calcium Urea 

• Temperature, Lactate & More 

SAMPLE PREPARATION 

• Spectrometers 

• HTS Plate Readers 

• Amino Acid Analysis 

ASK FOR A FREE GUIDE 

Send email to: 

regen@harvardapparatus.com 

28 


PHASE 3: Culturing & Growth 

Introduction to Phase 3 

Tools, Equipment & Systems 

This section contains systems and tools for the regeneration and growth of engineered or natural scaffolds and cellular 

constructs. Complete bioreactor systems typically include one or more growth chambers for the construct as well as fluid 

handling pathways for culture media perfusion and cell seeding. Probes for monitoring physiological parameters are typically 

included, though these are discussed in more detail among the Phase 4 products. 

Cell Culturing & Tissue Growth 

1. Cell & Tissue Preparation 

2. Cell Growth 

- Media Infusion Pumps 

a. physiological 

b. continuous flow 

- Imaging Chambers 

3. Cell Sorting 

- Capillary Glass 


4. Cell Harvesting 

5. 3D Bioreactors 

6. Spinner Flasks 

7. Centrifuges 

8. Pipetting Systems 

9. Tissue Culture 

10. Incubators 

11. Shakers 

12. Continuous flow pumps 




InBreath 3D Bioreactor for Hollow Organs, 

Bronchus, Trachea & Blood Vessels 

Proven through Research and Human Clinical Transplantation 

InBreath 

from the culture compartments. The connection between the motion unit and 

the culture chamber allows the first to remain in the incubator for the whole 

culture period, moving the chamber independently every time is needed (i.e. 

sampling, medium exchange). An external control unit regulates and monitors 

rotation. Autoclavability, ease of handling under sterile conditions, reliability 

and precision ensure the full compatibility of the device with the GLP rules 

(Good Laboratory Practice). 

Benefits & Features 


• Facilitates cell seeding procedures on both 

sides of a 3D tubular matrix, ensuring 

homogeneous plating 

• Proven Design - demonstrated regeneration of 

human bronchus and successful human 

transplantation with positive clinical outcome; 

as recently published by Macchiarini et al 

(2008) Clinical transplantation of a tissueengineered 

airway, The Lancet, Volume 372, 

Issue 9655 

• Allow seeding and culturing of different cell 

types on either side of the tubular scaffold 

• Enhance oxygenation of the culture medium 

and mass transport (oxygen, nutrients and 

catabolites) between the medium and the 

adhering cells 

• Stimulates the cells with hydrodynamic stimuli, 

favoring the metabolic activity and the 

differentiation process 

• Allows the achievement and maintenance of 

sterility and other criteria of Good Laboratory 

Practice (GLP), simplicity and convenience 

• Permits the possibility of automation and 

scale-up/-out 

In Breath is a rotating, double-chamber, bioreactor designed for cell seeding 

and culturing on both surfaces of a tubular matrix and includes rotational 

movement of the scaffold around its longitudinal axis. A polymeric culture 

chamber houses the biologic sample and the medium for the whole culture 

period. Cylindrical scaffold holders are constructed with working ends of 

different diameters - to house matrices of diverse dimensions - and a central 

portion of smaller diameter to expose the luminal surface of the matrix for cell 

seeding and culturing. A co-axial conduit links the inner chamber to the 

external environment through an appropriate interface at the chamber wall. 

This provides access to seed and feed the luminal surface of the construct. 

Secondary elements moving with the scaffold holder induce continuous mixing 

of the culture medium to increase oxygenation and mass transport. The 

cell/matrix construct is moved by a DC motor (0-5 rpm adjustable) separated 

Easy-to-use Controller 

• Compact design for remote placement 

(i.e. outside the incubator) 

• The rotational speed can be controlled from 

0 to 5 rotations per minute 

• Motor overload indicator for safe operation 

• CE certified 

Purpose-Built Reactor Chamber 

(Custom configurations available) 

• Reactor container and spindle are made of 

Polysulphone, Teflon and 316 Stainless Steel 

allowing for: 

- sterilization 

- chemical inertness 

- biological compatibility 

- transparent for excellent visualization 

• Chamber has quick-fit spindle for easy removal 

of the spindle and organ construct for analysis 

or dissambly 

• Rotating spindle assures even exposure to 

nutrient media 

• Integrated ports on spindle allow access to 

internal (lumen) surface of organ 

• Compact dimensions allow placements of 

multiple units within standard incubator 

• Offset cover allows for oxygenation of sample 

by non sheering ambient air contact 

• Quick release and disassembly of parts makes 

sterilization easy 

30 


InBreath 3D Bioreactor for Hollow Organs, 

Bronchus, Trachea & Blood Vessels 

(continued) 

InBreath 

Though it integrates several sophisticated features, the design of the InBreath 

is straightforward producing a very effective 3D Bioreactor. 

Figure 1: Tissue-specific tissue spindles ensure proper fit to the rotating core 

for even exposure to bathing media. 

Figure 2: Sealable access port through 

the chamber wall creates a conduit to the 

hollow-milled tissue spindle enabling 

clean access to the bronchial lumen for 

seeding, in this case, with endothelial 

cells from the host. 

Note: Images Courtesy of P. Macchiarini, 

University of Barcelona and S Mantero, 

Politecnico di Milano 


Rotational Speed 

Diagnostics 

Materials 

Validation 

Power 

0 - 5 RPM 

Positional Monitoring 

Polysulphone, Teflon, 318 Stainless Steel 

CE 

100 - 240 VAC, 50/60 Hz 

Figure 3: The semi-circular 

“half pipe” chamber floor 

creates a shallow well for 

external seeding, |here with 

host chondrocytes derived from 

bone marrow stem cells of the 

host. Mixing posts on the 

rotating tissue spindle maintain 

cells in suspension for even 

deposition on the bronchial 

surface. (Figure 3) 


73-4145 InBreath 3D Bioreactor for Hollow Organs, Bronchus, 

Trachea & Blood Vessels 

InBreath Harvard Apparatus | Advanced Solutions for Regenerative Medicine 

References: Macchiarini et al (2008) Clinical transplantation of a tissueengineered 

airway, The Lancet, Volume 372, Issue 9655 

Note: For Research Use Only. Not for use in humans unless proper 

investigational device regulations have been followed.” 


NEW LB-2 Bioreactor for Mouse and 

Rat Lung Regeneration 


LB-2 Bioreactor 

LB-2 Bioreactor 

For regeneration and respiratory monitoring of Mouse and 

Rat Lungs from decellularized scaffolds 

Benefits & Features: 

• Proven design – demonstrated regeneration of 

rat lung with physiological performance and 

subsequent transplantation, as recently 

published by Ott et al, 2010, Regeneration and 

orthotopic transplantation of a bioartificial lung 

, Nature Medicine, Volume 16 No 8, pgs 927-933 

•Active control of both airway ventilation and 

vascular perfusion during regeneration 

•On-line monitoring of respiratory mechanics and 

vascular physiology 

•Self-contained bioreactor specially designed for 

unique lung physiology 

Physiological maintenance and monitoring: 

The LB-2 provides an optimal, physiologically relevant environment for organ 

growth as well as the ultimate foundation for real-time monitoring of 

respiratory mechanics. The complete range of pulmonary monitoring parameters 

are fully integrated into the bioreactor to enable physiological monitoring during 

organ generation and end-stage validation for transplant. 

• Airway Flow 

• Airway Pressure 

• Intrapleural Pressure 

• Tidal Volume 

• Filtration Coefficient 

• Minute Volume 

• pH, pO 2 

, pCO 2 

• Pulmonary Resistance • Temperature 

• Pulmonary Compliance • Lung Weight 

• Pulmonary Artery Pressure 

• Pulmonary Venous (Left Atrial) Pressure 

• Vascular Resistance 

The LB-2 represents the evolution of legendary Harvard Apparatus - Hugo Sachs 

Elektronik perfusion technology from acute ex-vivo perfusion to Bioreactor 

based Organ Regeneration. Over 60 years of whole organ physiological 

perfusion experience are at the core of this first commercially available 

bioreactor for Lung Regeneration. 

Like all Harvard Apparatus - Hugo Sachs Elektronik perfusion systems, the LB-2 

provides unmatched physiological maintenance and monitoring capabilities 

thanks to the patented Solid State Physiological Perfusion Circuit (S2P2C) 

technology. In the LB-2, this means rigidly milled pathways, directly into the 

Perspex structure, for both vascular perfusion and airway ventilation. The result 

is precisely repeatable non-turbulent perfusion and respiration characteristics. 

This combined with the naturally excellent thermal properties of Perspex, 

creates a system that allows control, maintenance, and monitoring of vascular 

and respiratory mechanics during regeneration in a way that is more 

physiologically relevant than any conventional bioreactor. 

System Accessories: 

• Small Animal Ventilator 

• Pulsate Blood Pump 

• Syringe Pump 

• Transducers 

• Amplifiers 

• Data Acquisition and Analysis 

References: Ott et al (2010) Regeneration and orthotopic transplantation of a 

bioartificial lung, Nature Medicine, Volume 16 No 8 

32 


NEW LB-2 Bioreactor for Mouse and 

Rat Lung Regeneration (continued) 

The LB-2 is a Complete Lung Bioreactor for all phases of 

Lung Regeneration 

Phase 1: Decellularization 

Figure 1: Perfusion decellularization of whole rat lungs. (a) Photographs of a 

cadaveric rat lung, mounted on a decellularization apparatus allowing antegrade 

pulmonary arterial perfusion. pa, pulmonary artery; pv, pulmonary vein; tr, trachea; 

RUL, right upper lobe; RML, right middle lobe; LL, left lobe. Freshly isolated lung 

(left), after 60 min of SDS perfusion (middle), and after 120 min of SDS perfusion 

(right). The lung becomes more translucent as cellular material is washed out first 

from apical segments, then from the middle segments and finally from the basal 

segments. 

Phase 4: Transplantation 

Figure 3: Orthotopic 

transplantation and in 

vivo function. (a) 

Photograph of left rat 

chest after anterior 

thoracotomy, left 

pneumonectomy, and 

orthotopic 

transplantation of a 

regenerated left lung 

construct. Recipient left 

pulmonary artery (A), 

left main bronchus (B) 

and left pulmonary vein 

(V) are connected to 

regenerated left lung 

pulmonary artery (a), 

bronchus (b) and 

pulmonary vein (v). 

White arrowheads, the 

recipient’s right lung 

(infracardiac and right lower lobe); black arrowheads, the regenerated left lung 

construct. (b) Radiograph of rat chest after left pneumonectomy and orthotopic 

transplanation of a regenerated left lung constuct. White arrowheads, recipient’s 

right lung; black arrowheads, regenerated left lung construct. (c) Results of blood 

gas analyses showing decrease in arterial oxygen tension (PaO2) after left 

pneumonectomy and partial recovery after orthotopic transplantation of a 

regenerated left lung construct. Baseline, single lung, 10 min and 30 min 

measurements were obtained with the rat intubated with the extubated and 

breathing room air without support of a ventilator. Upper P values compare single 

lung ventilation to 5 min, and 30 min time points after transplantation at 5 min, 

30 min and 6 h time points after operation. Error bars, s.d. 

Phase 2: Regeneration 

Phase 3: Validation 

Figure 2: In vitro functional testing of regenerated lung constructs. (a) 

Photographs of regenerated lung contructs attached to the isolated lung 

apparatus) pa, pulmonary arterial cannula; pv, pulmonary venous cannula; tr, 

trachea) in expiration (left) and inspiration (right; RUL, right upper lobe; RML, right 

middle lobe). (b) Bar graphs showing results of blood gas analyses of pulmonary 

venous effluent of native lung, HUVEC- and A549-seeded lung groups showed gas 

exchange function; pO2/FiO2 ratio and pCO2 did not differ between native and 

H-FLC-seeded lungs. (c) Line chart showing the dynamic pressure/volume 

relationship during five respiratory cycles of native, decellularized and regenerated 

lungs. Corresponding compliance values (ml/cm H2O/s are shown in boxes. (d) Bar 

graph comparing vital capacity of native (black), H-A549-seeded lung (gray) and H- 

FLC-seeded, regenerated lung (red). Error bars, s.d. 

Order # 

Product 

73-4213 LB-2 Basic Unit for Lung Bioreactor, Size 2 

Note: Images and data courtesy of H. Ott, Massachusetts General Hospital 

LB-2 Bioreactor Harvard Apparatus | Advanced Solutions for Regenerative Medicine 


HPC-3, Hydrostatic Perfusion Chamber for Organ 

and Tissue Decellularization 


HPC-3 

Detergent or enzyme based decellularization of tissues and organs can be a 

cumbersome process, with multiple bottles of different solutions all leading to 

the tissue through a convoluted tubing network. The HPC-3 is a practical 

solution to this problem, adding useful features and enabling standardization of 

decellularization protocols for any tissue. Three independent non-heated glass 

vessels are mounted on a robust vertical stand to provide the required 

hydrostatic perfusion pressure and all required tubing, in appropriate lengths, is 

included. Glass vessels and the included Teflon Lids are fully autoclavable. 


73-001020 3-Fold Hydrostatic Perfusion Chamber with Stand 

34 


Cell and Tissue Perfusion, Imaging & 

Assay Chambers 

Universal Coverslip Chamber 

Ideal for live cell imaging 

experiments and cellular 

electrophysiology 

The NaviCyte 

Diffusion/Ussing 

Chamber Systems 

The “World Standard” for conducting substance transport studies across 

cultured cell monolayers or excised tissue under dynamic conditions. 

• Higher Throughput Screening 

–Up to 24 Chambers 

• Easier to Use than Other Systems 

Applications Include: 

Drug Delivery 

Toxicology 

Pharmacokinetics 

Metabolic Studies 

Transport Studies 

Cell & Tissue Perfusion Harvard Apparatus | Advanced Solutions for Regenerative Medicine 


Flow Through Devices for Removal of Toxins, 

Metabolites or Signaling Products 

Imaging Chambers 

Now sterilizable, in-line dialysis system to de-toxify 3D bioreactor perfusate. 

Take out DMSO, salts, and cell communication signal molecules so you can 

keep the 3D bioreactor perfusate in safe limits for organ regeneration 

The Flow-Thru DIALYZER is a unique system for the rapid dialysis of sample 

volumes from 20 µl to 100 ml. It provides a large surface area for Flow-Thru online 

dialysis with minimal sample loss. The entire dialysis unit is made of 

Teflon, an inert material, and has two separate serpentine channels 

superimposed on each other and separated by a dialysis membrane. The length 

of each channel is about 700 mm. Five different chambers are available (20 µl, 

75 µl, 150 µl, 300 µl and 600 µl). Chambers of different volumes can also be 

superimposed on each other for specific applications. 

Flow-Thru DIALYZER & Membranes 

A. Regenerated Cellulose MEMBRANES: 

1k Da MWCO 

2k Da MWCO 

5k Da MWCO 

10k Da MWCO 

25k Da MWCO 

Dialysis 

Buffer In 

Dialysis 

Buffer Out 

50k Da MWCO 

B. Cellulose Acetate MEMBRANES: 

100 Da MWCO 

Dialysis 

Buffer 

Chamber 

500 Da MWCO 

1k Da MWCO 

2k Da MWCO 


Sample 

Solution In 

Sample 

Solution Out 

Membrane 

(1.2 in x 3.2 in) 

Sample 

Chamber 

5k Da MWCO 

10k Da MWCO 

25k Da MWCO 

50k Da MWCO 

100k Da MWCO 

C. Polycarbonate MEMBRANES: 

0.01 um 

0.05 um 

0.60 um 

Filtration or Solid Phase 

Extraction 

In-Line PEEK cartridge for Toxic removal 

• Fast 

• Cost-effective 

• Preparation of microfluidic volumes 

Until now, commercially available options for preparing microliter volumes have 

suffered major drawbacks, including high back pressure, clogging, leakage and 

limited surface chemistry options. LabSmith’s new CapTite cartridges offer 

customizable packing and re-use, for easy sample preparation in both 

prototypes and high-throughput, automated systems. 

36 


PHASE 4: 

Physiological Assessement & 

Conditioning Monitoring 

Introduction to Phase 4 

Tools, Equipment & Systems 

Monitoring and recording of Physiological and Metabolic parameters, or BIOSENSING, is critical in understanding the viability of 

organisms, organs, tissues and cells and is the ultimate determinant of organ validation for transplant. 

Harvard Apparatus has probes and systems for more physiological and metabolic measurements than anyone else. 

We have tried to list all of our BIOSENSORS and in the following pages, we have noted some of the newest. 

For more details or for a specific request, please contact our technical specialists at regen@harvardapparatus.com. 

Cell, Tissue, Organ & Regenerative Systems 

Condition Monitoring Tools 

Cells 

1. Microscopic Imaging & 

Perfusion Chambers 

2. Microscopic Environmental 

Controls 

3. Electrophysiology Products 

4. Cell Condition Monitors 

Tissues 

5. Isolated Tissue & Perfusion 

Baths 

Organs 

6. Lung Regeneration Bioreactor 

7. 3D Bioreactors – Hollow 

Organs 

Sensors 

8. Ion Sensors 

Ammonia, Calcium, Chloride, 

Ethanol, Glucose, Lactate, 

Nitrate, Nitric Oxide, Nitrite, 

Peroxide, Potassium, Sodium, 

Urea 

9. Flow 

10. Temperature 

11. Pressure 

12. Force 

13. O 2 

; CO 2 


Capnograph 


A Range of Harvard Apparatus BIOSENSING 

Probes are Available for Virtually Any Metabolic or 

Physiological Measurement 

Biosensing 


Application Areas 

• Respiratory Mechanics 

• Cardiovascular Physiology 

• Absorption 

• Metabolism 

• Excretion 

• Nutrition 

• Electrophysiology 

• Biopotentials 

• Animal Behavior 

• Dosing 

Measurement Probes 

• Blood Pressure Transducers 

• Force Transducers 

• Differential Air Pressure Transducers 

• Flow Probes 

• Capnographs 

• Pulse Oxymeters 

• Temperature Monitors 

• Thermocouples 

• ECG Electrodes 

• Monophasic Action Potential Electrodes 

• Weight Transducers 

• Electrical Stimulation Electrodes 

• Imaging devices 

• Blood Gas Probes, pH, O 2 

, CO 2 

• Ion & Compound Sensors 

- Ammonia 

- Calcium 

- Chloride 

- Ethanol 

- Glucose 

- Lactate 

- Nitrate 

- Nitric Oxide 

- Nitrite 

- pH 

- Peroxide 

- Potassium 

- Sodium 

- Urea 

• Infrared Motion Detectors 

Control Unit 

Battery Powered Spill 

Sensor System 

Spill Sensor Mats with 

Connecting Cable 

• Protect your microscope 

• Audible alarm 

• TTL cutoff circuitry 

• Compatible with upright and inverted 

microscopes 

• Easy set up and installation 

• Complete system 

• Force Measurement 

• Digital Microscopy 

• Automated Blood Gas 

and Temperature 

Control 

• Air Pressure 

• pCO 2 

, pO 2 

, pH 

• Nitrate & Nitrite Ions 

• Oxygen levels in 

bulk fluids 

38 


NEW Optical Detection of pH, O 2 

and CO 2 

are 

Ideal for Bioreactor Use 

Optical Biosensors 

• Completely Sterilizable sensors withstand CIP 

(Clean-In-Place), SIP (Steam-In-Place), gamma, 

and autoclave conditions. 

• USP Class VI-certified sensors are shipped precalibrated 

although the system allows the user 

to perform a simple standardization procedure. 

Now pH, O 2 

and CO 2 

can be measured easily in disposable systems using new 

iDots. iDots are welded into the wall of the bioprocess container, are precalibrated, 

can be gamma sterilized and are made of all USP Class VI materials. 

iDots are readily adaptable to your single-use application using Polestar’s DSP 

series optical monitors. 

Flow-cell probe is a zero dead-volume sensor that can be up with DSP 

series Optical Process Monitors for continuous measurement of DO or pH. 

Pre-calibrated DO or pH fluorescent sensing films deposited along the inner 

surface of the quartz flow-cell body enabling optical interrogation through the 

transparent wall of the flow-cell via standard fiber optic cable. A simple 

re-useable clamping device with integral fiber optic connector is provided to 

facilitate mounting the device. Flexible silicone tubing on each end of the flowcell 

enables quick connection to external tubing or adapters such as Luer-Lock 

fittings. All materials used in the construction of the flow-cell sensors are USP 

Class VI certified and can withstand autoclave or gamma sterilization. 

Puncture probe oxygen sensors are configured for measurements of oxygen in 

applications such as package integrity testing, small volume samples and soft 

tissue monitoring. The probe is constructed from a small diameter fiber optic 

capped with a thin layer of oxygen sensing chemistry fixed within the lumen of 

a stainless steel hypodermic needle. Coring and non-coring designs are 

available in sizes ranging from 14 gauge to 24 gauge. Responses times (t90) for 

Polestar’s puncture probes are

Perfusion Solution Monitor 

Universal Perfusion Solution Monitor: proven 

electrochemical detection of pH, pO 2 

, pCO 2 

in 

hydrodynamic systems 

PH, pO 2 

, & pCO 2 

System Detail of pH, pO 2 

& pCO 2 

Sensor System 


Universal Perfusion 

Solution Monitor 

• Rugged construction 

• Noise free design, no cross talk 

• High sensitivity 

• Good linearity 

For studies involving isolated or in situ perfused organs (heart, lung, liver, 

kidney) it is often important to continuously monitor pO 2 

, pCO 2 

and/or pH of the 

perfusate or effluate. Which parameter is measured depends on the organ used 

and on the type of experiment conducted. 

The universal perfusion solution monitor permits precise continuous or 

discontinuous measurement in liquid media of these three key parameters pO 2 

, 

pCO 2 

and pH. The electrodes are all side stream flow-through electrodes and 

require a pulsation-free roller pump to deliver constant flow of perfusate 

through the electrode at flow rates in the range from 0.5 to 2 ml/min. The 

solution is pulled through each of the electrodes in series. The pH sensor 

requires an external reference electrode (both the pO 2 

and pCO 2 

contain 

internal reference electrodes). A solid state leak free reference system is used. 

Because of the high impedance of these sensors, screening or shielding of the 

measuring circuit is required to guard against electrostatic discharges and 

other electrical disturbances. The shielding case also provides a convenient 

point of attachment to the multi-electrode mounting plate. Sensors are 

available individually or configured as a complete package with suitable 

amplifiers and Data Aquisition. 

Detail of pO 2 

Sensor 

40 


Guide for Advanced Metabolic and Physiological 

Evaluation of Organisms, Organs, Tissues & Cells 

using Infrared Contrast Imaging (IRCI) 

Physiological Fluidics Monitoring 

Vascularization 

Disease Monitoring with Enhanced Visualization 

Microscopy Harvard Apparatus | Advanced Solutions for Regenerative Medicine 




using Infrared Contrast Imaging (IRCI) (continued) 

Microscopy 


Know More About Your Experiment with IRCI 

Harvard Apparatus has utilized infrared imaging in a way that not only provides temperature imaging but also produces high contrast images in biological and 

medical applications. This new technique uses the camera’s ability to detect IR emmisivity from objects as a function of their surface texture, composition, 

temperature, shape and more. This new utilization of infrared imaging has been called IRCI - Infrared Contrast Imaging. This powerful technology can visualize, 

with high resolution, experimental conditions based on tissue vascularization, structure or metabolic state. 

• Site evaluations for viable or necrotic tissue 

• More clearly reveal morphological features of biological substrates 

• Track injection flow paths in real time to evaluate injection quality 

Accurate Noncontact Infrared Measurement For Visualizing 

Physiological, Metabolic and Morphological features with IRCI 

To assure accurate noncontact infrared temperature measurement keep in mind the following: 

• Distance to Target (Spot) Ratio 

• Field of View 

• Environmental Conditions 

• Ambient Temperatures 

• Emissivity 

Distance to Target Ratio 

The distance controls the energy hitting the detector, the closer the camera the more signal being picked up. 

Field-of-view 

The array must have the correct lens in front of it to get as much illumination of the emitted light onto as many pixels as possible. When choosing a system the lens 

should be selected to give you the proper working distance and the magnification to display the area of interest on as much of the array as possible 

Environmental Conditions 

Watch for environmental conditions in the working area. Steam, dust, smoke, etc., can prevent accurate measurement by obstructing the unit's optics. Dew point or 

the moisture in the air can cause blurring of the image also if you have glass containers or certain plastics they will absorb all the IR energy so they are not 

transparent to the IR and you will not be able to see through these. If this happens replace the material with a IR transparent material if possible. 

Ambient Temperatures (the surrounding temperature) 

If the thermometer is exposed to abrupt ambient temperature differences of 20 degrees or more, allow it to adjust to the new ambient temperature for at least 20 

minutes. The camera fixed-mounted sensors are specified for performance within certain ambient temperature ranges. For high ambient temperatures, some 

camera’s offers air cooling and water cooling options as well as accessories, such as Thermojacket. 

Emissivity 

Emissivity is the measure of an object's ability to emit infrared energy. Emitted energy indicates the temperature of the object. Emissivity can have a value from 0 

(shiny mirror) to 1.0 (blackbody). 

Emissivity Differences Create Contrast with IRCI 

IRCI has the ability to enhance contrast of objects based on their emmissivity. There are many features of many different types of samples which may be revealed by 

IRCI, making it a particularly useful tool. Some of these features are: 

• Surface Texture 

• Sample Thickness 

• Temperature 

• Chemical Composition 

• Depth Profile 

42 




using Infrared Contrast Imaging (IRCI) (continued) 

Application Example: IRCI for Evaluation of Stem Cell Injection Site Physiology 

Infrared contrast imaging (IRCI) enables clear visizualization of physiological and structural features of living tissue which are otherwise very difficult to detect. 

This is demonstrated in a rodent model where cardiac infarct was induced via ligation of the left anterior descending coronary artery (LAD). 

Areas of advanced cardiac infarct generally display a faint and localized “paleness” at the epicenter of the infarct, where myocardial injury is greatest and most 

pronounced. The images below show, widespread changes in detected emmissivity on the myocardial surface after LAD ligation. What may be partially visible 

to an experienced observer using the naked eye, is now readily apparent to even the uninitiated user of IRCI technology. As a result, this technique enables 

routine procedures to be done without extensive training and with greater accuracy. 

Rat Orientation 

Infarct 

Area 

Handheld Digital Microscope with IR illumination 

• Pocket sized 3.5" LCD display/image capture 

• Real time image or video 

• Storage and playback 

• Up to 200X variable magnification 

• Built-in 8-Always-On white LEDs lighting (Option near UV light) 

• Microscope Resolution 628 x 586 

• LCD Screen Resolution 320 x 240 

Heart 

Infarct 

Area 

Time Zero to Infarct 30 Seconds After Infarct One Minute After Infarct 

Heart 

Infarct 

Area 

Heart 

Small, Compact and Powerful 

The Harvard Apparatus T-Series of portable infrared cameras 

takes infrared camera ergonomics, weight and ease-of-use to a 

new level. Usability is key 

Ideal for visualizing changes in vascularization or surface 

features of tissue and organs with otherwise low contrast. 

Microscopy Harvard Apparatus | Advanced Solutions for Regenerative Medicine 

i40 / i50 / i60 

• Economical IR camera for monitoring temperature 

IR Microscope 

• Imaging of 100 micron area at 3 micron resolution 


Digital Microscopy 0-500X with Integral 

Illumination: Small, Powerful and Economical 

Microscopy 

AM2011 

• Handheld Microscope 

• 0.3M/Resolution 640x480 

• USB 2.0 Output 

• 10~200X Various Magnification 

• 1/4" Color CMOS 

• Built-in 4 white LED’s 

AM311SAM311ST 

(Micro Touch) 





• LED on/off controlled by software 

• AM311ST with 

Trigger Button 

AM412N AV/TV output 

AM412NT LED On/Off 

AM412NZT Polarize 

AM412NTL Enhanced WD 

AM412MNT Aluminium Alloy 

AM412MNTL Aluminium Alloy 



• AV (Video)/TV Output 


AM313/313T 





• Measurement feature include (*) 



AMH-RUT 

• Handheld Iris Microscope 

• 1.3 MP 


• Resolution 1280x1024 

• USB 2.0 


• 10x -20x Various Magnification 

AM311H Earscope 

AMH-EAN AV/TV output 

AMH-EUT-V3 Earscope 1.3MP 

• Handheld Earscope 

• 0.3 MP / 1.3 MP 

• USB 2.0 Output / or TV 

• 10~50X / 55X~90X Magnification 

• Built-in 4/6 white LED’ 

AMH-N5UT 

AMB-DT-U1 USB 5X~37X 

• Handheld Iris Microscope 

AMB-DT-U2 USB 10X~92X 

• 1.3 MP 

AMB-DT-T1 AV/TV Polarizer 

• Resolution 1280x1024 

AMB-DT-T3 AV/TV 10X~92X 

• USB 2.0 

• Handheld Dental Scope 


• 0.3 MP (TV) 1.3 MP (USB) 

• 500X fixed Magnification 

• USB 2.0 Output / or TV 

• Various Magnification 

• Built-in 8 white LED’ 

AMK4012-C200 

• Handheld Digital Microscope 

• Pocket sized 3.5" LCD display/image capture 

• Real time image or video Storage and playback 

• Up to 200X variable magnification 

• Built-in 8-Always-On white LEDs lighting (Option near UV light) 

• Microscope Resolution 628x586 

• LCD Screen Resolution 320x240 

44 


References 

Our extensive references cover all areas of 

interest for Regenerative Medicine 

APPLICATION GUIDES: 

• BILAYER WORKSTATION 

• BOLUS INJECTIONS 

• CELL INFUSION 

• ELECTRODE 

• CULTURE DISH FEEDING 

• DRUG INJECTIONS 

• ELECTROSPINNING SCALFOLDS 

• IMAGING 

• ISOLATED HEART SYSTEM 

• NANOFLUIDICS 

• STEM CELL INJECTIONS 

• STEM CELL COLLECTION AND HARVESTING 

• USSING CHAMBER 

• VENTILATING ANIMALS 

Sample Research Publications: 

1. Spontaneous hair cell regeneration in the mouse utricle following gentamicin ototoxicity ..BIOREACTOR K Kawamoto, M Izumikawa, LA Beyer, GM … - Hearing Research, 2009 - Elsevier... if missing hair 

cells can be replaced by a regenerative treatment ... Infusion speed was controlled by a syringe pump (Harvard Apparatus Inc., Holliston, MA) at the ... 

2. Regenerative medicine bioprocessing: concentration and behaviour of adherent cell …BIOREACTOR BJH Zoro, S Owen, RAL Drake, C Mason, M … - Biotechnology and Bioengineering, 2009 - 

interscience.wiley.com ... Received March 27, 2009; Accepted April 3, 2009 ... for primary cells typical in regenerative medicine. ... vertically onto a syringe Pump (PHD2000, Harvard Apparatus... 

3. Towards microfabricated biohybrid artificial lung modules for chronic respiratory support…BIOREACTOR /Materials KA Burgess, HH Hu, WR Wagner, WJ … - Biomedical Microdevices, 2009 - Springer... 

215 McGowan Institute for Regenerative Medicine, University ... Biomed Microdevices (2009) 11:117–127 121 ... PHD 2000 syringe pump (Harvard Apparatus, Holliston, MA ... 

4. An automated system for delivery of an unstable transcription factor to hematopoietic stem …BIOREACTOR E Csaszar, G Gavigan, M Ungrin, C Therien, … - Biotechnology and Bioengineering, 2009 - 

interscience.wiley.com... Ontario, Canada 7 McEwen Centre for Regenerative Medicine, University ... ß 2009 Wiley Periodicals, Inc ... a Model 33 Twin Syringe Pump (#553333, Harvard Apparatus, St ... 

5. Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cell… BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … Engineering Part C: …, 

2010 - liebertonline.com... 16 Issue 3: May 26, 2010 Online Ahead of Print: February 26, 2010 Online Ahead ... Cell SortingAnalysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. ... 1 Hz,4 

ms, 50–100 V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA ... 

6. BMI1 sustains human glioblastoma multiforme stem cell renewal…STEM CELL cjb.net [HTML]M Abdouh, S Facchino, W Chatoo, V … - Journal of …, 2009 - neuro.cjb.net... This work revealed that PcG 

proteins are required in human GBM to sustain cancer-initiatingstem cell renewal. Materials and Methods. ... The scalp was closed with wound clips (Harvard Apparatus). Animals were followed daily for development of 

neurological deficits. ... 

7. In vivo multimodal imaging of stem cell transplantation in a rodent model of Parkinson's disease…STEM CELL J Jackson, C Chapon, W Jones, E Hirani, A … - Journal of neuroscience …, 2009 - Elsevier... 

6-Hydroxydopamine (6-OHDA) was injected into the right striatum at a dose of 16 g, in a volume of 4 L at a rate of 1 L/min, using a micropipettor (Harvard Apparatus, Edenbridge, UK). ...Post-implantation stem cell 

localisation was assessed using high-resolution MR images. ... 

8. Effects of ischemic preconditioning on regenerative capacity of hepatocyte in the …STEM CELL A Bedirli, M Kerem, H Pasaoglu, O Erdem, E … - Journal of Surgical Research, 2005 - Elsevier... model have 

shown that initiation of the regenerative response depends ... and the common bile duct with a microvascular clamp (Harvard Apparatus, Inc., Hollinston ... 

9. Development of microfluidics as endothelial progenitor cell capture technology for …STEM CELL BD Plouffe, T Kniazeva, JE Mayer Jr, SK … - The FASEB Journal, 2009 - FASEB... using a Harvard Apparatus 

PHD 2000 syringe pump (Harvard Apparatus, .23 October 2009 ... EPCs are a potential tool in regenerative medicine and likely ... 

10. Substantial detrusor overactivity in conscious spontaneously hypertensive rats with …STEM CELL LH Jin, KE Andersson, YH Kwon - Scandinavian Journal of Urology and Nephrology, 2009 - 

informaworld.com... 2 Wake Forest Institute for Regenerative Medicine, Wake ... ISSN 1651-2065 online # 2009 Informa UK ... a micro- injection pump (PHD22/2000 pump; Harvard Apparatus). ... 

11. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELLS/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com... Received 

14 February 2009; Accepted 9 December 2009; Published online 7 February 2010. ...analyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... Cell fusion by Electro cell manipulator (ECM 

2001, BTX Harvard Apparatus) and incubated ... 

12. Pilot study to investigate the possibility of cytogenetic and physiological changes in bio- …SCAFFOLD MATERIALS/ electrospinning H Kempski, N Austin, A Roe, S Chatters, SN … - Regen. Med., 2008 

- Future Medicine... 3 s -1 (PHD 4400, HARVARD Apparatus Ltd., Edenbridge ... Materials for Tissue Engineering & Regenerative Medicine2,158 ... (2009) Bio-electrospraying embryonic stem cells ... 

13. In vivo bone tissue engineering using mesenchymal stem cells on a novel electrospun …SCAFFOLD MATERIALS/ electrospinning M Shin, H Yoshimoto, JP Vacanti - Tissue engineering, 2004 - 

liebertonline.com... Q 5 0.1 mL/min, PHD 2000 syringe pump; Harvard Apparatus, Holliston, MA ... 2009. Nanostructured polymer scaffolds for tissue engineering and regenerative medicine ... 

14. Silk fibroin microtubes for blood vessel engineering…SCAFFOLD MATERIALS nih.gov M Lovett, C Cannizzaro, L Daheron, B … - Biomaterials, 2007 - Elsevier... c Center for Regenerative Medicine, 

Massachusetts General Hospital, Harvard Medical School ... the bioreactor using a syringe pump (Harvard Apparatus, Holliston, MA ... 

15. Quantitative Analysis of Neural Stem Cell Migration and Tracer Clearance in the Rat Brain by MRI…STEM CELL JA Flexman, DJ Cross, LN Tran, T Sasaki, Y Kim, S … - Molecular Imaging and … - 

Springer... DOI: 10.1007/s11307-010-0311-3 Mol Imaging Biol (2010) ... morphologically transplanted cell migration in the rat brain and parametrically estimate neural stem cell migration speed. ... the skull) using a 

picoliter syringe pump (Pico Plus Syringe Pump; Harvard Apparatus; Holliston ... 

16. Therapeutic effect of genetically engineered mesenchymal stem cells in rat experimental leptomeningeal glioma model. STEM CELL C Gu, S Li, T Tokuyama, N Yokota, H Namba - Cancer letters, 2009 - 

Elsevier... Mesencult ® murine mesenchymal stem cell medium) after aspiration by 5 ml syringe connected with 23 G needle ... the subarachnoid space by a 50 µl microsyringe (Hamilton Company, Reno, NV) connected 

with a 27 G needle and a microinjector (Harvard Apparatus, Inc., South ... 

16. Magnetosonoporation: Instant magnetic labeling of stem cells… STEM CELL B Qiu, D Xie, P Walczak, X Li, J Ruiz- … - Magnetic …, 2010 - interscience.wiley.com... VC 2010 Wiley-Liss, Inc ... basal medium 

(Stemcells Inc., Vancouver, Canada), adding NeuroCult supple- ments for neural stem cell differentiation and ... Using a nanoinjector (Harvard Apparatus, Holliston, MA), the left brain hemispheres were locally implanted 

with approximately 8 ... 

17. A seeding device for tissue engineered tubular structures BIOREACTOR L Soletti, A Nieponice, J Guan, JJ Stankus, WR Wagner … - Biomaterials, 2006 - Elsevier... The tees were connected to a precision 

syringe pump (Harvard Apparatus Inc.,Holliston, MA, USA) outside the chamber by means of hydraulic rotating joints (DeublinCo., Waukegan, IL, USA) and polyvinyl chloride (PVC) tubing. ... 

References Harvard Apparatus | Advanced Solutions for Regenerative Medicine 


References 

References 


18. A novel culture system shows that stem cells can be grown in 3D and under physiologic pulsatile conditions for tissue engineering of vascular grafts 171.65.102.190….BIOREACTOR [PDF]O Abilez, 

P Benharash, M Mehrotra, E … - Journal of Surgical …, 2006 - Elsevier... system that would allow future testing of mechanical, biochemical, and thermal stimuli on stem cell differentiation into ... (B) Photo of the 

bioreactor (left) and ... The pulsatile pump was a Harvard Apparatus Model 1405 (Harvard Apparatus, Holliston, MA) modified for computer control... 

19. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization…BIOREACTOR M Lovett, D 

Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation,and ... Medford, MA 02155. E- 

mail: Received: April 19, 2010 Accepted: June 1, 2010. ... syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ... 

20. A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cells BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … Engineering Part C: …, 

2010 - liebertonline.com... Activated Cell Sorting Analysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. MDSC-3D collagen gel bioreactor (MDSC-3DGB) construction ... 1 Hz, 4 ms, 50–100 

V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA). ... 

21. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLD MATERIAL 

F Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte, and mesenchymal stem 

cell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ... 

22. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLD 

MATERIALS F Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte, and 

mesenchymal stem cell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ... 

23. Porous nanocrystalline silicon membranes as highly permeable and molecularly thin substrates for cell culture …SCAFFOLD MATERIAL AA Agrawal, BJ Nehilla, KV Reisig, TR Gaborski, DZ … - 

Biomaterials, 2010 - Elsevier... Received 8 March 2010; accepted 16 March 2010. Available online 15 April 2010. Abstract. ...An open perfusion microincubator (Harvard Apparatus) was used to maintain cells at 37 °C and 

mineral oil was floated on top of the media to minimize evaporation. ... 

24. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning… SCAFFOLD MATERIAL nih.gov [HTML]F Teulé, AR Cooper, WA Furin, D Bittencourt, EL … - Nature 

protocols, 2009 - nature.com... Bacteria have quicker generation times and do not require a very sophisticated laboratory setup,thus reducing time and expenses. Additionally, the production can be easily scaled-up using 

bioreactors. ... no. P-259); Harvard Pump 11 plus single syringe (Harvard Apparatus, cat. ... 

25. Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials …SCAFFOLD MATERIAL nisco.ch [PDF]SN 

Jayasinghe, N Suter - Biomicrofluidics, 2010 - link.aip.org... Biomicrofluidics 4, 014106 (2010)]. ... glass microslides, while for scanning electron microscopy (SEM) and transmission electron microscopy (TEM), fibers were 

collected ... at the lowest possible flow rate allowed by our present syringe pump, (PHD4400, Harvard Apparatus Ltd., Kent ... 

26. Characterization and tensile strength of HPC-PEO composite fibers produced by electrospinning …SCAFFOLD MATERIAL L Francis, A Balakrishnan, KP Sanosh, E Marsano - Materials Letters, 2010 - 

Elsevier ... strength of HPC-PEO composite fibers produced by electrospinning, Materials Letters (2010), doi: 10.1016/j ... PEO, Sigma Aldrich, Mw = 10,000) were used as starting precursors to fabricate nano fibers. ... of 

250 m and was mounted on a syringe pump (Harvard Apparatus serial n A ... 

27. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELL/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com ... karyotype 

analyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... h, inactivated feeder cells and fresh media were added, and the culture was then ...2-cell fusion by Electro cell manipulator 

(ECM 2001, BTX Harvard Apparatus) and incubated ... 

28. Osteochondral Interface Tissue Engineering Using Macroscopic Gradients of Bioactive Signals …STEM CELLS NH Dormer, M Singh, L Wang, CJ Berkland, MS … - Annals of biomedical …, 2010 - 

Springer ... 0090-6964/10/0600-2167/0 © 2010 Biomedical Engineering Society ... There is a growing interest in hUCMSCs as a mesenchymal stem cell source, as they have been ... in a controlled manner using 

programmable syringe pumps (PHD 22/2000, Harvard Apparatus, Inc., Holliston ... 

29. Engineered Early Embryonic Cardiac Tissue Increases Cardiomyocyte Proliferation by Cyclic Mechanical Stretch via p38-MAP Kinase Phosphorylation …BIOREACTOR nih.gov [HTML]KC Clause, 

JP Tinney, LJ Liu, BB Keller, … - … Engineering Part A, 2009 - liebertonline.com ... 1 Hz, 4 ms, and 70–100 V) using a stimulator (Harvard Apparatus, Holliston, MA). ... Pasumarthi, KB, Soonpaa, MH, and Field, LJ Myocyte 

and myogenic stem cell transplantation in ... A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle ... 

30. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CC Anamelechi, 

EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation for three-dimensional 

polymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ... 

31. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CC Anamelechi, 

EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation for three-dimensional 

polymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ... 

32. Regeneration and orthotopic transplantation of a bioartificial lung-BIOREACTOR HC Ott, B Clippinger, C Conrad, C Schuetz, I … - Nature Medicine, 2010 - nature.com ... Received 22 December 2009 Accepted 

04 July 2010 Published online 13 July 2010 ... black arrowheads), axial (gray arrowheads) and septal (black arrows) elastic fibers in decellularized ...trachea and was placed in a sterile, water-jacketed organ chamber 

(Harvard Apparatus). ... 

33. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org …BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006... 1. A lowmagnification 

micrograph of an entire well is shown in Figure 5. Also on post-seeding day 1, the cell culture medium was ... These syringes were loaded into a programmable pump capable of supporting 10 individual 

syringes (PHD2000, Harvard Apparatus, Holliston, MA ... Cited by 53 - Related articles - All 9 versions 

34. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization …BIOREACTORS M Lovett, D 

Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and ... the 

collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ... 

35. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett, D 

Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... tube embedded within the collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard 

Apparatus, Holliston, MA). For oxygen measurements,a custom housing for the bioreactor and oxygen measurement probe was ... 

36. Cardiac tissue engineering using perfusion bioreactor systems …BIOREACTOR nih.gov [HTML]M Radisic, A Marsano, R Maidhof, Y Wang, G … - Nature protocols, 2008 - nature.com... only within the 

construct, that is, at the construct outlet oxygen concentration stops varying as a function of bioreactor length ... 97063A134), coated with PTFE by Microsurfaces; Stereomicroscope; Sterilization pouches; Syringe pump 

(Push/Pull; Harvard Apparatus or WPI Instruments ... 

37. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett, D 

Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Medford, MA 02155. E-mail: Received: April 19, 2010 Accepted: June 1, 2010. ... 4 µL/min) of the silk tube embedded within the 

collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA). ... 

38. Distribution of NTPDase5 and NTPDase6 and the regulation of P2Y receptor signalling in the rat cochlea nih.gov …BIOREACTOR [HTML]MG O'Keeffe, PR Thorne, GD Housley, SC Robson, … - 

Purinergic …, 2010 - Springer... Purinergic Signalling (2010) 6:249–261 251 Page 4. ... Plastic tubing was placed inside the inlet and outlet holes and both tubes were connected to separate Hamilton syringes secured on a 

push–pull syringe pump (Harvard Apparatus, USA). ... 

39. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org…BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006 -These syringes 

were loaded into a programmable pump capable of supporting 10 individual syringes (PHD2000, Harvard Apparatus, Holliston, MA). ... demonstrated that cocultured primary hepatocytes and fibroblasts in a flat plate 

bioreactor were not adversely effected by wall ... 

46 


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