Gel Doc™ XR ChemiDoc™ XRS

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Note: When using the White Light, you must turn ON the UV Transilluminator after removing the sample. 12. The system will automatically acquire a reference image and a flat-fielded corrected image of the sample will be generated for you to save and analyze. 13. For additional details on Flat Fielding please refer to the Quantity One® Software Manual. You may also click on the Quantity One® HELP button for a detailed description. 5.2 Cutting gels 1. Open the drawer until it is fully extended and the UV Trans LED stops blinking. 2. Place the clear acrylic UV protection screen in the horizontal slot in the inside of the drawer facing the transilluminator. !! CAUTION!! !! VORSICHT !! WARNING: EXPOSURE TO UV RADIATION IS HAZARDOUS TO HEALTH. PLEASE WEAR PROPER UV PROTECTIVE CLOTHING AND FACE AND EYE SHIELDS WHEN CUTTING GELS. THE UV SHIELD PROVIDED WITH THE SYSTEM IS NOT ADEQUATE PROTECTION AGAINST UV GENERATED BY THIS SYSTEM. 3. Press the UV Trans button to turn on the UV illumination. 4. Cut the gel. 5. Press the UV Trans button and the UV transilluminator will turn off. Remove the acrylic UV protection shield before closing the drawer. Page 28 of 42
Section 6 Trouble Shooting Problem Possible Cause Solution Image is not visible on the monitor • Aperture is closed. • Incorrect monitor settings • Wrong cable connections. Image is not bright enough. • Wrong aperture setting • Insufficient exposure time Impossible to image whole sample area. Printout does not look the same as the image on the monitor. Light leakage into the darkroom. • Lens is zoomed-in too close. • Monitor settings are wrong. • Thermal printer settings are wrong • The lens body is not properly centered and against the light seal gasket on the hood • Τhe rear hole on the MZL not closed with the screw. • Open the aperture. • See your computer manual • See Appendix A or B for correct cabling. • Open the aperture • Select longer exposure time • Zoom-out the lens. • Read the manual for the printer to ensure proper settings • Loosen the bracket and move the Camera around to properly seal the opening on the hood or adapter plate as the case may be. • Close the hole with the black plastic screw. Hot (very bright) pixels are seen in the image (ChemiDoc XRS only) Hot (very bright) pixels are seen in the image (Gel Doc XR only) ChemiDoc XRS Camera will not respond ChemiDoc XRS image will not Auto expose Unable to focus on the sample using White Light transilluminator or conversion screen • “Reference” is not selected for “Dark Subtraction” in the OPTIONS window • Corrupted “Reference Dark” image • Power may be off • Controller cable may not be seated properly • Software Driver for the Camera is missing • Camera may be defective • The sample is too bright and saturates the image at the minimum possible exposure • Aperture is open too wide causing a shallow depth of field • Select “Reference” by clicking on the appropriate box in the OPTIONS window. • Click on “OPTIONS” button and then click on “RESET REFERENCE DARK” • Turn ON the power to the Camera • Make sure that Camera controller cable is connected properly • Install Quantity One® again if the driver is not present • Replace Camera • Close the IRIS using the IRIS control until Auto expose is possible. • Close the Iris slightly and then focus again. Keep repeating until good focus is achieved Page 29 of 42
Page 1 and 2: Gel Doc XR ChemiDoc XRS 170-8170
Page 3 and 4: Regulatory Notice IMPORTANT: The Ge
Page 5 and 6: TABLE OF CONTENTS HARDWARE INSTRUCT
Page 7 and 8: Section 1 Introduction The Bio-Rad
Page 9 and 10: Section 3 Product Description 3.1 C
Page 11 and 12: Part # Descriptions 170-8170/170-81
Page 13 and 14: 4.2.2 Assembling the CCD Camera Gel
Page 15 and 16: Center the lens in the opening of t
Page 17 and 18: Setting Up the Lens: 1. Turn off th
Page 19 and 20: The Imaging led blinks when you are
Page 21 and 22: Filters: A +1 Diopter is factory in
Page 23 and 24: 1. Make sure that all lights in the
Page 25 and 26: Section 5 Operation of the Universa
Page 27: 10. Remove the sample from the tran
Page 31 and 32: 100-2784 UV transilluminator Lid (I
Page 33 and 34: This unit is protected by 2 fuses 5
Page 35 and 36: Appendix A: Gel Doc XR System Cable
Page 37 and 38: Appendix C: Installing Software and
Page 39 and 40: Gel Doc XR and ChemiDoc XRS- Mac: a
Page 41 and 42: Appendix D: PCI Card Installation P

Gel Doc™ XR ChemiDoc™ XRS

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