Marine Ecosystems Research Department - jamstec japan agency ...

More documents

Recommendations

Info

Japan Marine Science and Technology Center Frontier Research System for Extremophiles Fig. 8 Morphological properties of Sulfurihydrogenobium subterraneus A FISH for S. subterraneus-specific probe B Whole microbial cells stained with DAPI C Electron micrograph of S. subterraneus Persephonella hydrogenophila sp. nov. A novel thermophilic, hydrogen-oxidizing bacterium, designed as W T , was isolated from a deep-sea hydrothermal vent chimney sample collected from the Suiyo Seamount in the Izu-Bonin Arc, Japan, at a depth of m. The cells were coccoids (.-. mm in diameter) and straight rods (.-. mm long) under static and agitated culture conditions, respectively. The new isolate was an obligate chemolithoautotroph growing by respiratory nitrate reduction with H , forming N as a final product. Very low concentration of O (opt. .-. %, v/v) was also used as an alternative electron acceptor while reduced sulfur compounds did not serve as electron donors. Growth was observed between and .˚C (opt. ˚C; min doubling time), pH . and . (opt. pH .), and in the presence of . and . % NaCl (opt. .%) on anoxic hydrogen-oxidizing growth with nitrate. The G+C content of the genomic DNA was . mol%. Phylogenetic analysis based on S rDNA sequence indicated that the isolate was a member of the recently described genus Persephonella in a potential new family within the order Aquificales. On the basis of the physiological and molecular properties of the new isolate, the name Persephonella hydrogeniphila sp. nov. is proposed. The type strain is strain W T (= JCM T = DSMZ T ). Marinithermus hydrothermalis sp. nov. A novel thermophilic marine bacterium, designated as strain T T , was isolated from a deep-sea hydrothermal vent chimney sample collected from the Suiyo Seamount in the Izu-Bonin Arc, Japan, at a depth of m. The cells were found to be rod-shaped, occurring in pairs or filamentous, and stained gramnegative. Growth was observed between and .˚C (opt. .˚C; min doubling time) and pH . and . (opt. pH .). The new isolate absolutely required NaCl in the range between . and . % NaCl (opt. %). It was a strictly aerobic heterotroph capable of growing solely on the complex organic substrates such as yeast extract, tryptone and Casamino acid, and utilizing glutamate, proline, serine, cellobiose, trehalose, sucrose, acetate, and pyruvate as complementary substrates. The G+C content of the genomic DNA was . mol%. The S rRNA gene (rDNA) sequence of the isolate was most similar to those of members of the genus Thermus but distantly related to them on the genus level of differentiation (< %). In addition, the phylogenetic analysis 83
JAMSTEC 2002 Annual Report Frontier Research System for Extremophiles indicated that the new isolate was a novel lineage deeply branched prior to divergence of the genus Thermus. On the basis of the phylogenetic and physiological traits of the new isolate, it should be described as the new genus differentiated from the previously described genus of Thermus. The name Marinithermus hydrothermalis gen. nov., sp. nov. is proposed. The type strain is strain T T (=JCM T =DSM T ). Deferribacter desulfuricans sp. nov. A novel anaerobic, heterotrophic thermophile was isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Izu-Bonin Arc, Japan. The cells were bent, flexible rods, with a single polar flagellum. Growth was observed between and ˚C (optimum temperature: –˚C; -min doubling time) and between pH . and . (optimum: pH .). The isolate was a strictly anaerobic heterotroph capable of using complex organic compounds (yeast extract, tryptone, peptone, casein and Casamino acid), ethanol and various organic acids as energy and carbon sources. Hydrogen can serve as a supplementary energy source. Elemental sulfur (S ), nitrate and arsenate are required for growth as electron acceptors. The G+C content of the genomic DNA was . mol%. Phylogenetic analysis based on S rDNA sequences indicated that the isolate is closely related to Deferribacter thermophilus strain BMA T . This isolate, however, could be clearly differentiated from D. thermophilus strain BMA T on the basis of its physiological and genetic properties. We propose the name Deferribacter desulfuricans sp. nov. (type strain: SSM T ; JCM and DSM ). Isolation of many novel, previously uncultivated epsilon-Proteobacteria Successful cultivation and partial characterization of novel members of ε-Proteobacteria, which have been long recognized solely as genetic signatures of small subunit ribosomal RNA genes (rDNA), are reported from a variety of microbial habitats occurring Other Bacterial Phyla 45 0.05 55 47 56 68 in deep-sea hydrothermal fields. Based on the phylogenetic analysis of the rDNA from the isolates, most of them represent the first cultivated members within the previously uncultivated phylotypes classified into the Uncultivated ε-Proteobacteria Group A, B, F and G, as well as some novel members of the Group D (Fig. ). Preliminary characterization of the isolates indicates that all of the isolates are mesophilic or thermophilic chemolithoautotrophs using H or reduced sulfur compounds (elemental sulfur or thiosulfate) as an electron donor and O , nitrate or elemental sulfur as an electron acceptor. The successful cultivation will lead to the subsequent characterization of physiological properties and ecological impacts of a diversity of ε-Proteobacteria in the global deep-sea hydrothermal environments. 30 62 24 95 37 69 23 38 74 83 20 65 83 34 39 67 76 53 34 100 97 60 45 89 32 31 61 90 84 NT CSE NKB9 AB013261 OTIN DHVE str. 42BKT + 2 strains Alvinella pompejana epibiont clone L35521 OTIN DHVE str. E9I37-1 MAR DHVE VC2.1-Bac4 AF068786 OTIN DHVE str. BKB55-1 + 1 strain MAR DHVE VC1.2-Cl10 AF367486 47 MAR DHVE VC1.2-Cl07 AF367485 MAR DHVE VC1.2-Cl04 AF367483 OTIN DHVE str. BKB25Ts-Y + 1 strain SEPR DHVE S17sBac16 AF299121 SEPR DHVE S17sBac17 AF299122 OTIN DHVE str. B455-1 + 1 strain MAR DHVE VC1.2-Cl21 AF367489 GB DHVE str. EX-18.2 AF357196 EPR DHVE str. Am-H AF357197 Nautilia lithotrophica str. 525 AJ404370 MAR DHVE VC2.1-Bac30 AF068804 MAR DHVE VC2.1-Bac17 AF068795 Caminibacter hydrogeniphilus str. AM1116 AJ309655 58 51 51 SEPR DHVE S17sBac14 AF299124 Caminibacter hydrogeniphilus str. AM1115 AJ309654 SEPR DHVE S17sBac5 AF299130 Alvinella pompejana epibiont clone L35520 Rimicaris exoculata epibiont clone U29081 OTIN DHVE str. E9S37-1 + 2 strains OTIR DHVE str. 49MY MAR DHVE VC2.1-Bac1 AF068783 62 85 94 45 81 MAR DHVE VC1.2-Cl42 AF367493 CIR DHVE str. 365-55-1% + 5 strains 99 MAR DHVE VC1.2-Cl01 AF367481 OTIN DHVE str. B155-1 + 9 strains OTIN DHVE str. MI55-1 + 1 strain Sulfurospirillum barnesii AF038843 Sulfurospirillum deleyianum Y13671 GB DHVE str. EX-18.1 AF357199 EPR DHVE str. Am-N AF357198 100 Arcobacter cryaerophilus L14624 Arcobacter skirrowi L14625 Wolinella succinogenes AF273252 Helicobacter pylori U01328 Flexispira rappini M88137 100 Campylobacter jejuni Y19244 "Bacteroides ureolyticus" L04321 Thiovulum sp. M93223 62 CIR DHVE str. 1-37-1% + 1 strain NT CSE NKB11 AB013263 65 CIR DHVE str. 2-37-10% Thiomicrospira denitrificans L40808 72 OTIN DHVE str. GO25-1 MAR DHVE VC1.2-Cl51 AF367496 91 39 MAR DHVE VC1.2-Cl26 AF367490 63 MAR DHVE VC1.2-Cl68 AF367498 69 OTHK str. OK-5 + 2 strains 69 OTHK str. OK-10 + 58 strains 56 MAR DHVE VC1.2-Cl49 AF367494 Uncultivated Epsilon Proteobacteria Group C Uncultivated Epsilon Proteobacteria Group G Uncultivated Epsilon Proteobacteria Group D Uncultivated Epsilon Proteobacteria Group F Uncultivated Epsilon Proteobacteria Group A Sulfurospirillum Group Arcobacter Group Helicobacter Group Campylobacter Group Uncultivated Epsilon Proteobacteria Group B Fig. 9 Phylogenetic tree of representative 16S rRNA gene sequences from cultivated strains and deep-sea hydrothermal vent clones within ε-Proteobacteria. 84
Page 2 and 3:
JAMSTEC 2002 Annual Report J apan M
Page 4 and 5:
Japan Marine Science and Technology
Page 6 and 7:
Page 8 and 9:
Page 10 and 11:
Page 12 and 13:
Page 14 and 15:
Page 16 and 17:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
JAMSTEC 2002 Annual Report Marine T
Page 28 and 29:
JAMSTEC 2002 Annual Report Marine T
Page 30 and 31: JAMSTEC 2002 Annual Report Marine T
Page 32 and 33: Japan Marine Science and Technology
Page 48 and 49: JAMSTEC 2002 Annual Report Marine E
Page 112 and 113: JAMSTEC 2002 Annual Report Frontier
Page 130 and 131:
JAMSTEC 2002 Annual Report Frontier
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
1000 Japan Marine Science and Techn
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Page 168 and 169:
Page 170 and 171:
Page 172 and 173:
Page 174 and 175:
Page 176 and 177:
Page 178 and 179:
JAMSTEC 2002 Annual Report Mutsu Re
Page 180 and 181:
JAMSTEC 2002 Annual Report Mutsu Re
Page 182 and 183:
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
JAMSTEC 2002 Annual Report Research
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 202 and 203:
Page 204 and 205:
Page 206 and 207:
Page 208 and 209:
Page 210 and 211:
Page 212 and 213:
Page 214 and 215:
Page 216 and 217:
Page 218 and 219:
Page 220 and 221:
Page 222 and 223:
Page 224 and 225:
Page 226 and 227:
Page 228 and 229:
Page 230 and 231:
Page 232 and 233:
Page 234 and 235:
Page 236 and 237:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
Page 244 and 245:
Page 246 and 247:
Page 248 and 249:
Page 250 and 251:
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274 and 275:
Page 276 and 277:
JAMSTEC 2002 Annual Report PATENT .
Page 278:
JAMSTEC Japan Marine Science and Te
show all

Marine Ecosystems Research Department - jamstec japan agency ...

Create successful ePaper yourself

Delete template?

Save as template?