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Marine Ecosystems Research Department - jamstec japan agency ...

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JAMSTEC 2002 Annual Report<br />

<strong>Marine</strong> <strong>Ecosystems</strong> <strong>Research</strong> <strong>Department</strong><br />

a<br />

δ 18 O (‰)<br />

-3<br />

Pf<br />

-4<br />

→<br />

Mf<br />

→<br />

→ Kf<br />

b<br />

→<br />

→<br />

Ms<br />

Ks<br />

→<br />

-3<br />

Ps -4<br />

Tf<br />

Ts<br />

-5<br />

-5<br />

Fast growing<br />

Pf’<br />

Ps’ Slow growing<br />

-6<br />

-6<br />

-5 -4 -3 -2 -1 0 -5 -4 -3 -2 -1 0<br />

δ 13 C (‰) δ 13 C (‰)<br />

Fig.16 The compositional changes in the carbon and oxygen stable isotopes. The fast-growing coral (a) is<br />

characterized as a large contribution of metabolic isotope effect ( M f ) compared with kinetic isotope<br />

effect ( K f ) relatively. The slow-growing coral (b) is characterized as a small contribution of metabolic<br />

isotope effect ( M s ) compared with kinetic isotope effect ( K s ).<br />

effects such as photosynthesis. This model might<br />

improve the past environmental condition and biogenic<br />

carbonate.<br />

a<br />

b<br />

<strong>Research</strong> on phenotypic diversity of benthic arborescent<br />

organisms<br />

Period: FY-FY<br />

The sea area around Japan contain a great diversity<br />

of benthic fauna such as Octocorallia and black coral<br />

species. Unlike hermatypic coral species, which can<br />

only live in shallow tropical and subtropical waters,<br />

Octocorallia also inhabits the high-latitude regions and<br />

deep-sea areas. In this research I verify the reproductive<br />

patterns that adapt to low water temperatures to<br />

confirm factors through which this coral has extended<br />

its habitat from shallow sea areas to the deep sea, and<br />

from the low to the high latitudes. The purpose of this<br />

research is to gain a phylogeographical understanding<br />

of species classification. It was conducted that the<br />

research on the reproductive pattern and period and<br />

genetic diversity for gorgonian species (Octocorallia)<br />

inhabiting shallow waters, and build a taxonomic base<br />

for species inhabiting the deep sea.<br />

In fiscal it was conducted that the analysis on<br />

the classification and morphometry based on existing<br />

data both for species of gorgonian (Octocorallia:<br />

Gorgonacea) that inhabit deep-sea areas and species<br />

that inhabit shallow waters (Fig.). It was carried<br />

out field surveys on species that live at depths of<br />

around m at the Otsuchi <strong>Marine</strong> <strong>Research</strong> Center,<br />

Ocean <strong>Research</strong> Institute, University of Tokyo, and<br />

Fig.17 (a) Melithaea flabellifera. (b) Primnoidae sample collected<br />

by the Otsuchi <strong>Marine</strong> <strong>Research</strong> Center, Ocean <strong>Research</strong><br />

Institute, University of Tokyo in fiscal 2002.<br />

examined their distribution in this area. It was also<br />

carried out taxonomic studies on samples collected by<br />

the Ocean <strong>Research</strong> Institute, University of Tokyo.<br />

These results were presented in scientific journals, and<br />

at conferences and symposiums in overseas.<br />

The establishment of method to measure the genome<br />

size using fluorophotometry<br />

Period: FY-<br />

Generally microbes with small genome size had<br />

been utilized to analyze whole genome. Recently,<br />

analysis of genome was expanded in humanbeing and<br />

some model organisms with large genome size.<br />

Pulsed-field gel electrophoresis was often used to<br />

measure the genome size. However, in this method the<br />

measurable genome size is restricted and much time<br />

and expensive reagents are needed to measure. On the<br />

other hand, fluorophotometry is a simple method but<br />

relatively high reliability in the measured value. In this<br />

method, however, not only DNA but also some other<br />

58

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