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Japan Marine Science and Technology Center Appendix E Budget Revenues FY 1984 FY 1985 FY 1986 FY 1987 FY 1988 FY 1989 FY 1990 FY 1991 FY 1992 FY 1993 FY 1994 FY 1995 FY 1996 FY 1997 FY 1998 FY 1999 FY 2000 FY 2001 FY 2002 Governmental Investment Non Governmental Investment Undertaking Income and others 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 billion (yen) Expenses FY 1984 FY 1985 FY 1986 FY 1987 FY 1988 FY 1989 FY 1990 FY 1991 FY 1992 FY 1993 FY 1994 FY 1995 FY 1996 FY 1997 FY 1998 FY 1999 FY 2000 FY 2001 FY 2002 Research Support Ship operation Salary and others 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 billion (yen) 289
Japan Marine Science and Technology Center Appendix F PATENT Microorganism belonging to the genus flavobacterium, hydrocarbon emulsifier and solubilizer and separation method for organic-solvent tolerant microorganism Assignee: Japan Marine Science and Technology Center, (), - Natsushima-cho, Yokosuka-shi, Kanagawa -, (JP), (Proprietor designated states: all) HOKKAIDO SUGAR CO., LTD, (), No:, chome Jinbocho, Kanda, Chiyoda-ku Tokyo, (JP), (Proprietor designated states: all) Inventor: Moriya, Kazuhito, -, -Chome , Takashimadaira, Itabashi-ku, Tokyo, (JP) Horikoshi, Koki, -, - Chome, Sakuradai, Nerima-ku, Tokyo, (JP) Patent Country Code/Number Kind Date EP 536046 A2 1993 (Basic) EP 536046 A3 1993 EP 536046 B1 2002 Application Country Code/Number Date EP 92402680 September 30, 1992 Priority Application Number (Country Code, Number, Date): JP (); JP () Designated States: DE; FR; GB International Patent Class: CN-/; CF- /; CN-/; CR-: Cited Patents (EP A): GB A; EP A; EP A; FR A; EP A Cited Patents (EP B): EP A; EP A; EP A; FR A; GB A Abstract: EP 536046 A2 A novel microorganism belonging to the genus Flavobacterium posessing the capacity to decompose hydrocarbons, tolerance to sulfurous acids, tolerance to salinity, tolerance to organic solvents, and tolerance to pressure. The novel microorganism is of a strain of the genus Flavobacterium DS- (FERM P-). A hydrocarbon emulsifier and solubilizer having as an active component thereof a water soluble and acetone insoluble fraction obtained by culturing DS- strain. A separation method for an organic-solvent tolerant microorganism, wherein a sample is mixed with water and an organic solvent, shaking culturing is conducted, a cultured mixture is allowed to stand, separation into an aqueous phase and an organic solvent phase is conducted, an appropriate amount of said organic solvent phase is added a culture medium and cultured, and microorganisms which grow therein are isolated. Claims: EP 536046 A3 . A novel microorganism belonging to the genus Flavobacterium possessing an ability to decompose hydrocarbons, tolerance to sulfurous acids, tolerance to salinity, tolerance to organic solvents, and tolerance to pressure. . The novel microorganism of claim , wherein said microorganism is DS- strain of the genus Flavobacterium. . A hydrocarbon emulsifier and solubilizer having as an active component thereof a water soluble and acetone insoluble fraction obtained by culturing DS- strain of the genus Flavobacterium (FERM P-) in a culture medium containing hydrocarbons. . A separation method for an organic-solvent tolerant microorganism, wherein a sample is mixed with water and an organic solvent, shaking culturing is conducted, a cultured mixture is allowed to stand, separation into an aqueous phase and an organic solvent phase is conducted, an appropriate amount of said organic solvent phase is added to a culture medium and cultured, and microorganisms which grow therein are isolated. . The method of claim , wherein said shaking culturing is conducted for a period of - days at a temperature of - (degree) C. 291
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