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Marine Ecosystems Research Department - jamstec japan agency ...

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JAMSTEC 2002 Annual Report<br />

<strong>Research</strong> Achievements<br />

host-vector system for deep-sea piezophilic bacteria,<br />

Shewanella violacea DSS and Moritella japonica DSK (in<br />

Japanese), JAMSTECR, , -, .<br />

) Hagihara, H., Igarashi, K., Hayashi, Y., Kitayama, K., Endo,<br />

K., Ozawa, T., Ozaki, K., Kawai, S. & Ito, S., Improvement of<br />

thermostability of a calcium-free a-amylase from an alkaliphilic<br />

Bacillus sp. by protein engineering, J. Appl. Glycosci,<br />

, -, .<br />

) Hakamada, Y., Endo, K., Takizawa, S., Kobayashi, T., Shirai,<br />

T., Yamane, T. and Ito, S., Enzymatic properties, crystallization,<br />

and deduced amino acid sequence of an alkaline endoglucanase<br />

from Bacillus circulans, Biochim. Biophys. Acta, ,<br />

-, .<br />

) Hamamoto, M., Nagahama, T. & Tamura, M., Systematic study<br />

of basidiomycetous yeasts - evaluation of the ITS regions of<br />

rDNA to delimit species of the genus Rhodosporidium, FEMS<br />

Yeast Res , -, .<br />

) Inagaki, F., Motomura, Y. & Ogata, S., Microbial silica deposition<br />

in geothermal hot waters, Appl. Microbiol. Biotechnol.<br />

, in press.<br />

) Inagaki, F., Sakihama, Y., Inoue, A., Kato, C. & Horikoshi, K.,<br />

Molecular phylogenetic analyses of reverse transcripted bacterial<br />

rRNA obtained from deep-sea cold seep sediments,<br />

Environ. Microbiol., , -, .<br />

) Inagaki, F., Sakihama, Y., Kato, C., Inoue, A. & Horikoshi, K.,<br />

Microbial diversity in the cold seep sediment at the Japan<br />

Trench, JAMSTEC J. Deep Sea Res., , -, .<br />

) Inagaki, F., Sakihama, Y., Takai, K., Komatsu, T., Inoue, A. &<br />

Horikoshi, K., Profile of microbial community structure and<br />

presence of endolithic microorganisms inside a deep-sea rock,<br />

Geomicrobiol. J. , -, .<br />

) Inagaki, F., Sakihama, Y., Takai, K., Komatsu, T., Inoue, A. &<br />

Horikoshi, K., Transition in microbial community structures<br />

and presence of unusual microorganisms in a deep-sea rock,<br />

Geomicrobiol. J. in press.<br />

) Inagaki, F., Takai, K., Hirayama, H., Yamato, Y., Nealson, K.<br />

H. & Horikoshi, K., Distribution and phylogenetic diversity of<br />

the subsurface microbial community in a Japanese epithermal<br />

gold mine, Extremophiles in press.<br />

) Ishii, A., Nakasone, K., Sato, T., Wachi, M., Sugai, M., Nagai,<br />

K. & Kato, C., Isolation and characterization of the dcw cluster<br />

from the piezophilic deep- sea bacterium Shewanella violacea,<br />

J. Biochem., , -, .<br />

) Ito, S., Hatada, Y., Ozawa, T., Hagihara, H., Araki, H., Tsujino,<br />

Y., Kitayama, K., Igarashi, K., Kageyama, Y., Kobayashi, T.<br />

and Ozaki, K., Protein-engineered Bacillus α-amylases that<br />

have acquired both enhanced thermostability and chelator<br />

resistance, J. Appl. Glycosci., , -, .<br />

) Ito, S., Kobayashi, T., Ozaki, K., Igarashi, K., Hagihara, H.,<br />

Hayashi, Y., Ozawa, T., Hatada, Y. and Kawai, S.,<br />

Carbohydrate-active enzymes from alkaliphiles, J. Appl.<br />

Glycosci., in press.<br />

) Kato, C., Nakasone, K., Ikegami, A., Kawano, H., Usami, R.<br />

and Horikoshi, K., Bacteria-sensing mechanisms at transcription<br />

level in piezophilic bacteria. J.S.B.B. Congress,<br />

Proceedings, in press.<br />

) Kato, C., Nakasone, K., Ikegami, A., Kawano, R., Usami, R.<br />

and Horikoshi, K., Molecular mechanisms of pressure-regulation<br />

at transcription level in piezophilic bacteria, In "Trends in<br />

High Pressure Bioscience and Biotechnology" (ed. R.<br />

Hayashi), pp.-, .<br />

) Kawano, H., Nakasone, K., Ikegami, A., Kato, C., Usami, R.<br />

& Horikoshi, K., Purification and characterization of RNA<br />

polymerase from deep-sea piezophilic Shewanella violacea.<br />

JAMSTECR, , -, .<br />

) Kawasaki, K., Nogi, Y., Hishinuma, M., Nodasaka, Y.,<br />

Matsuyama, H. & Yumoto, I., Psychromonas marina sp. nov.,<br />

a novel halophilic, facultatively psychrophilic bacterium isolated<br />

from the coast of the Okhotsk Sea, Int J Syst Evol<br />

Microbiol. , -, .<br />

) Kobayashi, T., Sawada, K., Sumitomo, N., Hatada, Y. & Ito,<br />

S., Bifunctional pectinolyti enzyme with separate pectate lyase<br />

and pectin methylesterase domains from an alkaliphilic<br />

Bacillus isolate, Curr. Microbiol., in press.<br />

) Koyama, S. & Aizawa, M., PKC dependent IL- production<br />

and inhibition of IL- production by PKC activation in normal<br />

human skin fibroblasts under extremely high hydristatic pressure,<br />

Extremophiles, , -, .<br />

) Koyama, S., Fujii, S. & Aizawa, M., Post-transcriptional regulation<br />

of immunomodulatory cytokines production in human<br />

skin fibroblasts by intense mechanical stresses, J. Biosci.<br />

Bioeng., , -, .<br />

) Koyama, S., Horii, M., Yanagida, Y., Kobatake, E., Miwa, T.<br />

& Aizawa, M., Pressure Sensitivity of HSP Promoter in<br />

Transformed T-L Cells, JAMSTEC Journal Deep Sea<br />

<strong>Research</strong>, .<br />

) Koyama, S., Miwa, T., Horii, M., Horikoshi, K. & Aizawa, M.,<br />

Tissue culture of the deep-sea eel Simenchelys parasiticus collected<br />

at m, Extremophiles, in press.<br />

) Koyama, S., Miwa, T., Horii, M., Ishikawa, Y., Horikoshi, K.<br />

& Aizawa, M., Pressure-stat aquarium system designed for<br />

capturing and maintaining deep-sea organisms, Deep Sea<br />

<strong>Research</strong> Part I, Oceanographic <strong>Research</strong> Papers , ,<br />

-, .<br />

) Mitsuzawa, S. & Yukawa, T., Reverse chemical evolution: A<br />

new method to search for thermally stable biopolymers,<br />

Origins Life Evol.Biosphere. in press.<br />

) Miura, T., Abe, F., Inoue, A., Usami R. and Horikoshi, K.,<br />

232

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