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Seed Testing of Maize and Wheat A Laboratory Guide - Search ...

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References<br />

Booth, C. 1971 . The Genus<br />

Fusarium. CMI, UK.<br />

Booth, C. 1977. Fusarium<br />

<strong>Laboratory</strong> <strong>Guide</strong> to the<br />

Identification <strong>of</strong> the Major<br />

Species. CMI, UK.<br />

Growth <strong>of</strong> colony on seed is rapid ,<br />

<strong>and</strong> the white aerial mycelium is<br />

light to dense, with orange spore<br />

masses appearing as the culture<br />

ages.<br />

Microconidia are formed initially<br />

from simple lateral conidiophores,<br />

<strong>and</strong> later from pr<strong>of</strong>usely branched<br />

conidiophores. Microconidia are<br />

hyaline, abundant, lemon- to pearshaped<br />

or spindle-shaped, 0-1<br />

septate, <strong>and</strong> <strong>of</strong>ten have a foot cell<br />

at the base, <strong>and</strong> measure:<br />

oseptate 7-11 x 4-8 IJm<br />

1 septate 10-16 x 4-6IJm.<br />

The most distinguishing<br />

characteristics are the abundant<br />

microconidia that are either<br />

lemon- to pear-shaped or broad in<br />

the middle <strong>and</strong> narrowing towards<br />

the ends. The shape <strong>of</strong> the<br />

microconidia distinguishes<br />

F. tricinctum from F. poae.<br />

McGee, D.C. 1988. <strong>Maize</strong><br />

Diseases: A Reference Source<br />

for <strong>Seed</strong> Technologists. APS<br />

Press, USA.<br />

Nelson, P.E., Toussoun, T.A ., <strong>and</strong><br />

Marasas, WF.O. 1983.<br />

Fusarium Species - An<br />

Illustrated Manual for<br />

Identification. The Pennsylvania<br />

State University Press, USA <strong>and</strong><br />

London.<br />

Macroconidia are abundant,<br />

hyaline, usually formed in pale to<br />

orange spore masses, sickleshaped,<br />

or more strongly curved,<br />

with a well-marked foot cell,<br />

3-5 septate, <strong>and</strong> measure<br />

26-53 x 3-5 IJm.<br />

Spherical chlamydospores (10­<br />

12 11m) are present <strong>and</strong> formed at<br />

intervals along hyphae, singly or<br />

in chains.<br />

Perithecial state is unknown.<br />

10

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