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Untitled - International Rice Research Institute

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orange. On the reverse side of the agar plate, the<br />

colony looks azonated and yellowish brown. At 21 °C<br />

under alternating 12-h NUV light and 12-h darkness,<br />

colonies are restricted in growth and attain a 3.96-cm<br />

diam in 15 d. They are azonated, plane, velvety with<br />

even to slight sinuate margins, pale orange, and<br />

moisture is produced with age. The colony on the<br />

reverse side of the agar plate appears azonated and<br />

yellowish brown. At 28 °C under alternating 12-h<br />

fluorescent light and 12-h darkness, colonies are restricted<br />

in growth and attain a 4.23-cm diam in 15 d.<br />

They are zonated, plane, velvety with sinuate margins,<br />

and pale orange. On the reverse side of the agar<br />

plate, the colony appears slightly zonated and yellowish<br />

brown.<br />

Colonies on PSA at ART (28–30 °C) are restricted<br />

in growth and attain a 4.80-cm diam in 15 d.<br />

They are slightly zonated, slightly felted with sinuate<br />

margins, and pale orange. The colony on the reverse<br />

side of the agar plate appears slightly zonated and<br />

pale yellow-orange. At 21 °C under alternating 12-h<br />

NUV light and 12-h darkness, colonies are restricted<br />

in growth and attain a 4.21-cm diam in 15 d. They<br />

are azonated, plane, slightly velvety, with a few<br />

slight radial furrows, sinuate margins, and pale orange.<br />

The colony appears azonated, with a few radial<br />

wrinkles and pale yellow-orange on the reverse<br />

side of the agar plate. At 28 °C under alternating 12-h<br />

fluorescent light and 12-h darkness, colonies are restricted<br />

in growth and attain a 4.05-cm diam in 15 d.<br />

They are slightly zonated, slightly felted with a few<br />

slight radial furrows in some plates, with slightly sinuate<br />

to even margins, and pale orange; moisture is<br />

produced with age. The colony appears slightly zonated<br />

with a few slight radial wrinkles and pale yellow-orange<br />

on the reverse side of the agar plate.<br />

Colonies on MEA at ART (28–30 °C) are restricted<br />

in growth and attain a 4.84-cm diam in 15 d.<br />

They are slightly zonated, plane, velvety, and pale<br />

orange. The colony appears slightly zonated and dull<br />

orange with pale yellow-orange margins on the reverse<br />

side of the agar plate. At 21 °C under alternating<br />

12-h NUV light and 12-h darkness, colonies are<br />

restricted in growth and attain a 4.37-cm diam in 15<br />

d. They are zonated, felted with slight sinuate margins,<br />

and pale orange. On the reverse side of the agar<br />

plate, the colony appears azonated and dull orange<br />

with pale yellow-orange margins. At 28 °C under<br />

alternating 12-h fluorescent light and 12-h darkness,<br />

colonies are restricted in growth and attain a 4.47-cm<br />

diam in 15 d. They are plain to felted, zonated at the<br />

center, and become azonated toward the margins,<br />

with slight radial furrows and slight sinuate margins.<br />

Colonies are pale orange and whitish toward the margins.<br />

On the reverse side of the agar plate, the colony<br />

appears zonated with a few radial wrinkles and dull<br />

orange with pale yellow-orange margins.<br />

Seedborne fungi causing grain and inflorescence diseases in rice<br />

Curvularia sp.<br />

Disease caused: black kernel<br />

a. Symptoms<br />

Black discoloration on grains.<br />

b. Occurrence/distribution<br />

Curvularia sp. is frequently isolated from discolored<br />

rice grains. Several species have been reported<br />

on rice from different countries (Fig. 38),<br />

but C. lunata and C. geniculata are the most common<br />

ones.<br />

c. Importance in crop production<br />

Curvularia sp. causes little or no yield loss under<br />

normal rice production situations. Infected grains,<br />

after being polished, may produce black kernels,<br />

thus reducing their market value.<br />

Detection on seed<br />

a. Incubation period on blotter<br />

On blotters incubated under NUV light at 21 °C,<br />

the fungus can be observed growing on rice seed<br />

7 d after seeding. The detection frequency is<br />

about 70.6% on seeds coming from different regions<br />

(Fig. 39a,b).<br />

b. Habit character<br />

Aerial mycelia are scanty or absent; if present,<br />

they are light brown to brown with abundant<br />

branching. Conidiophores are solitary or in groups;<br />

dark brown; straight, sometimes bent; simple;<br />

arising directly from the seed surface. Conidia are<br />

borne more or less at the tip in a whorl or in thick<br />

panicles (Fig. 40a-c).<br />

38

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