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Enginering of β casein Recombinant β casein, wild type, expressed in E. coli : WT Recombinant β casein, mutated, expressed in E. coli: MU MRELEEL RELEELNVPG EIVESLSSSE ESITRINKKI EKFQSEEQQQ TEDELQDKIH PFAQTQSLVY PFPGPIPNSL PQNIPPLTQT PVVVPPFLQP EVMGVSKVKE AMAPKHKEMP FPKYPVEPFT ESQSLTLTDV ENLHLPLPLL QSWMHQPHQP LPPTVMFPPQ SVLSLSQSKV LPVPQKAVPY PQRDMPIQAF LLYQEPVLGP VRGPFPIIV Sequence containing three glutamates well conserved in Ruminants Addition of three negative charges: change of hydrophilic/hydrophobic ratio The France-Egypt Year Of Science And Technology, 2010
Same conditions as for DLS. λmmax Trp 1 mg/mL 00,4 4 mg/mL λmaxTrp 0,2mg/mL λmaxTrp max Trp λm 342 340 338 336 334 332 342 340 338 336 334 332 Native Intrinsic Fluorescence of Tryptophan Tryptophan (1) (1) (2) 10 20 30 40 50 Temperature, °C WT 10 20 30 40 50 T emperature, t °C Temperature, °C λmmaxTrp max Trp λm The France-Egypt Year Of Science And Technology, 2010 342 340 338 336 334 332 MU 10 20 30 40 50 Temperature, °C
- Page 1 and 2: ENGINEERING OF DAIRY PROTEINS AND T
- Page 3 and 4: Average composition of f cow milk i
- Page 5 and 6: Caséine κ 169 aa 19037 Da 1 Chymo
- Page 7 and 8: P P P P β casein MKVLILACLV ALALAR
- Page 9: F I Trp 143 monomer λ ↑ °CC Int
- Page 13 and 14: SDS-PAGE of bovine β casein and mu
- Page 15 and 16: SDS-PAGE of the dimerizing β casei
- Page 17 and 18: Hydrophilic Hydrophilic-Hydrophilic
- Page 19 and 20: Chhaperone-like activity ac (%) Cha
- Page 21 and 22: Consequence of Cys incorporation on
- Page 23 and 24: − Average IgE (ng/ml) 35 30 25 20
- Page 25: Our INRA team Isabelle Bronnec Aynu
Same conditions as<br />
for DLS.<br />
λmmax<br />
Trp<br />
1 mg/mL<br />
00,4 4 mg/mL<br />
λmaxTrp<br />
0,2mg/mL<br />
λmaxTrp max Trp<br />
λm<br />
342<br />
340<br />
338<br />
336<br />
334<br />
332<br />
342<br />
340<br />
338<br />
336<br />
334<br />
332<br />
Native<br />
Intrinsic Fluorescence of<br />
Tryptophan Tryptophan (1) (1)<br />
(2)<br />
10 20 30 40 50<br />
Temperature, °C<br />
WT<br />
10 20 30 40 50<br />
T emperature, t °C<br />
Temperature, °C<br />
λmmaxTrp<br />
max Trp<br />
λm<br />
The France-Egypt Year Of Science And Technology, 2010<br />
342<br />
340<br />
338<br />
336<br />
334<br />
332<br />
MU<br />
10 20 30 40 50<br />
Temperature, °C
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