production of selected secondary metabolites in transformed ...
production of selected secondary metabolites in transformed ...
production of selected secondary metabolites in transformed ...
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F i<br />
90<br />
70<br />
50<br />
30<br />
10<br />
-10<br />
-30<br />
Zephyram<strong>in</strong><br />
Septonex Ajat<strong>in</strong><br />
DDAB<br />
Triton<br />
SDS<br />
-50<br />
0 0,002 0,004 0,006 0,008 0,01 0,012 0,014 0,016<br />
Fig.4.: Surfactants <strong>in</strong>fluence on Ga-HQS complex at pH 3. Septonex, Zephyram<strong>in</strong>e, Ajat<strong>in</strong>,<br />
SDS – mol.dm -3 , CTAC (Hexadecyl trimethyl ammonium chloride)– c/4 (mol.dm -3 ),<br />
DDAB (Didodecyldimethyl ammonium bromide) – c/5 (mol.dm -3 ), Triton X-100 –<br />
c/4.10 -5 (ppm), Brij – c/15 (mol.dm -3 ).<br />
Critical micellar concentrations are highlighted by the fulfilled marks.<br />
The nature <strong>of</strong> the complex strongly depends on the pH. The molar ratio <strong>of</strong> Me to QSA <strong>in</strong><br />
the b<strong>in</strong>ary complex determ<strong>in</strong>ed by the cont<strong>in</strong>uum variations method is 1:1 <strong>in</strong> acidic range and<br />
1:3 <strong>in</strong> alkalic range (Fig.5). In the presence <strong>of</strong> cationic surfactant Zephyram<strong>in</strong>e, the molar<br />
ratio <strong>of</strong> ternary complex was found to be always 1:3 (Fig.5).<br />
F i<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
0 0,1 0,2 0,3 0,4 0,5 0,6 0,7 0,8 0,9 1<br />
x L<br />
1<br />
2<br />
3<br />
c<br />
80<br />
60<br />
40<br />
20<br />
CTAC<br />
Brij<br />
a 100<br />
b<br />
F i<br />
0<br />
0 0,1 0,2 0,3 0,4 0,5 0,6 0,7 0,8 0,9 1<br />
Fig.5.: (a) Cont<strong>in</strong>uum variations <strong>of</strong> Al-HQS complex at pH 4. 1-c0= 1,1.10 -4 M, 2- c0= 5,6.10 -5 M,<br />
3-5,6.10 -5 M, 0,0012M Zephyram<strong>in</strong>e<br />
(b) Cont<strong>in</strong>uum variations <strong>of</strong> In-HQS complex. 1-c0= 3,5.10 -5 M, pH8, 2- c0= 1,8.10 -5 M,<br />
pH8, 3-1,8.10 -5 M, 0,0012M Zephyram<strong>in</strong>e, pH8, 4- c0= 1,8.10 -5 M, pH4<br />
F<strong>in</strong>ally, the improved method with enhanced sensitivity by the presence <strong>of</strong> cationic<br />
surfactant Zephyram<strong>in</strong>e was optimized with concentration <strong>of</strong> Zephyram<strong>in</strong>e 0,0012M and five<br />
times excess <strong>of</strong> QSA to highest molar concentration <strong>of</strong> Me. Optimal pH is 4 for Al, 3 for Ga<br />
and 8 for In.<br />
Sborník soutěže Studentské tvůrčí č<strong>in</strong>nosti Student 2006 a doktorské soutěže O cenu děkana 2005 a 2006<br />
Sekce DSP 2006, strana 246<br />
x L<br />
1<br />
4<br />
2<br />
3