production of selected secondary metabolites in transformed ...

Didodecyldimethylammonium bromide – Sigma-Aldrich Co.
Polyoxyethylene(23) lauryl ether (Brij 35) – Sigma-Aldrich Co.
Hexadecyltrimethylammonium chloride – Sigma-Aldrich Co.
4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol (Triton X-100) – Calbiochem Co.
APPARATUS
A single beam spectrofluorimeter Aminco Bowman ® , series 2 with a xenon lamp working
in a continuous regime, 4 nm slits and constant photomultiplier voltage was used for the
measurements.
RESULTS
The 8-hydroxyquinoline-5-sulphonic acid forms with Al, Ga and In fluorescent complex with
emission maxima at 495 nm (Al), 503 nm (Ga) and 505 nm (In). Excitation maxima for Al is
360 nm, 365 nm for Ga and 367 nm for In. There is no fluorescence observed for isolated
QSA. Excitation and emission spectra of Al-QSA in dependence on concentration of Al and
reaction blank without Al are shown in Fig.1.
The formation of fluorescent complex is instantaneous and fluorescence is stable at least for
twelve hours (Fig.3). Fluorescence intensity grows from In to Al and strongly depends on pH
(Fig.2).
F i
80
70
60
50
40
30
20
10
1a
2a
3a
4a
5a
6a
6b
0
270 320 370 420 470 520 570 620
Fig.1.: Excitation(a) and Emission(b) spectra of Aluminium(III) in the presence of 7,4.10 -5
mol.dm -3 QSA in dependence on concentration of Al(III).
1-1,6μg.cm -3 ,2-0,8μg.cm -3 , 3-0,4μg.cm -3 ,4- 0,2 μg.cm -3 ,5-0,05 μg.cm -3 ,6–0μg.cm -3
Sborník soutěže Studentské tvůrčí činnosti Student 2006 a doktorské soutěže O cenu děkana 2005 a 2006
Sekce DSP 2006, strana 244
λ (nm)
1b
2b
3b
4b
5b