production of selected secondary metabolites in transformed ...

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Addition of antioxidants, their amounts or combination have no influence on changes in oils during the storage. However, the way of storage can influence on the content of GLA. REFERENCES [1] Christie, W.W.: The analysis of evening primrose oil. Industrial Crops and Products, 1999, vol. 10, pp. 73-83. ISSN 0926-6690. [2] Court, W. A., Hendel, J.G., Pocs, R.: Determination of fatty acids and oil content of evening primrose (Oenothera biennis L.). Food Reearch International, 1993, vol. 26, pp. 181-186. ISSN 0963-9969. [3] Fan,Y.-Y., Chapkin, R.S.: Importance of dietary γ-linolenic acid in human health and nutrition. Recent Advantages in Nutritional Science, 1998, vol. 128, pp.1411-1414. ISSN 0022-3166. [4] Horrobin, D. F.: Nutritional and medical importance of gamma-linolenic acid. Progress In Lipid Research, 1992, vol. 31, pp. 163-194. ISSN 0163-7827. [5] ČSN EN ISO 5509: Animal and vegetable fats and oils – preparing of methyl esters of fatty acids, 2000. Sborník soutěže Studentské tvůrčí činnosti Student 2006 a doktorské soutěže O cenu děkana 2005 a 2006 Sekce DSP 2006, strana 242
IMPROVED FLUORIMETRIC DETERMINATION OF Al, Ga AND In BY MICELLE ENHANCED 8-HYDROXYQUINOLINE -5-SULPHONIC ACID COMPLEX Šimon Vojta, 3. ročník DSP Prof.RNDr.Lumír Sommer, DrSc. Faculty of Chemistry, Brno University of Technology, Institute of Chemistry and Technology of Environmental Protection, Purkyňova 118, 61200 Brno, e-mail: vojta@fch.vutbr.cz INTRODUCTION The enhancement (sensitization) of fluorescent reactions of metal ions with chelating dyes by the presence of surfactants provides an inexpensive alternative to fluorimetric methods, when determination of lower concentrations of elements is required. The complexes formed in micellar media are often characterized by high molar absorptivities, high stability over a wide pH range, and usually by large bathochromic shift caused by addition of surfactants to the binary complex formed in water. Moreover, some fluorescent binary chelates may dramatically increase their quantum yield in micellar media, providing exceptionally sensitive fluorimetric methods. The 8-hydroxyquinoline-5-sulphonic acid (QSA) should provide surfactant-sensitized reactions with Al(III), Ga(III) and In(III), because it is strong acid readily yielding a negatively charged group (sulphonate) to interact with the positive head of cationic surfactants. The importance of monitoring aluminium concentrations in the blood of patients undergoing haemodialysis has been realised because of the suspicion that aluminium intoxication is responsible for encephalopathy, oesteopathy and Alzheimer's disease. In this work, the influence of various surfactants on the reaction of QSA with Al, Ga and In is studied in details. Improved method of determination of Al, Ga, In by QSA in presence of cationic surfactant Zephyramine is brought and optimized. EXPERIMENTAL REAGENTS Aluminium standard, containing 1,000±0,002 g l -1 of Aluminium in 5% HCl, purchased from Analytica, LTD. Prague Gallium standard, containing 1,000±0,002 g l -1 of Gallium in 10% HCl, purchased from Analytica, LTD. Prague Indium standard, containing 1,000±0,002 g l -1 of Indium in 10% HCl, purchased from Analytica, LTD. Prague 8-hydroxyquinoline-5-sulphonic acid – Sigma-Aldrich Co. Benzyldimethyltetradecylammonium chloride (Zephyramine ® ) – Sigma-Aldrich Co. 1-ethoxykarbonylpentadecyltrimethylammonium bromide (Septonex ® )–Sigma-Aldrich Co. Dodecylbenzyldimethylammonium bromide (Ajatin ® ) – Sigma-Aldrich Co. Sborník soutěže Studentské tvůrčí činnosti Student 2006 a doktorské soutěže O cenu děkana 2005 a 2006 Sekce DSP 2006, strana 243
Page 1 and 2: PRODUCTION OF SELECTED SECONDARY ME
Page 3 and 4: containing ampicillin, IPTG and x-g
Page 5 and 6: THE SIMPLE METHOD FOR THE RECOGNITI
Page 7 and 8: Figure 1 Negative-ion mode MALDI-TO
Page 9 and 10: Table 1 Evaluation of negative-ion
Page 11 and 12: Fig. 1: Scheme of the hydride gener
Page 13 and 14: [4] H. Matusiewicz, M. Kopras, J. A
Page 15 and 16: - are hypotriglyceridemic; - exhibi
Page 17 and 18: HYDROPHOBIZED SODIUM HYALURONATE IN
Page 19 and 20: spectrophotometer (AMINCO-Bowman, S
Page 21 and 22: Hyaluronate derivatives showed with
Page 23 and 24: copolymers were additionally functi
Page 25 and 26: Each micelle has a hydrophobic core
Page 27 and 28: STUDY OF THE COPPER(II) IONS NON-ST
Page 29 and 30: CuSO4, Cu(ClO4)2 and Cu2P2O7 of the
Page 31 and 32: total flux [mol.m -2 ] 0.7 0.65 0.6
Page 33 and 34: number of free radicals is very low
Page 35 and 36: Differences can be caused by severa
Page 37 and 38: MEASUREMENT OF THERMOPHYSICAL PROPE
Page 39 and 40: The typical time responses of tempe
Page 41 and 42: Figure 4 illustrates the typical de
Page 43 and 44: sodium sulphate and filtered to a 5
Page 45: Compare all tested evening primrose
Page 49 and 50: Al F i 80 70 60 50 40 30 20 10 0 0
Page 51 and 52: F i 60 50 40 30 20 10 Data UCL LCL
Page 53 and 54: PHYSICAL-CHEMICAL ASPECTS OF PAINT
Page 55 and 56: Finally, the solubility parameter o
Page 57 and 58: Study of the influence of different
Page 59 and 60: Fig.1: Chemical structures of pI ma
Page 61 and 62: Identification of pI markers - Firs

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