production of selected secondary metabolites in transformed ...
production of selected secondary metabolites in transformed ...
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Table 1 Sum <strong>of</strong> the cac values for all samples<br />
sample SD cac<br />
- % x10 6 mol·l -1<br />
D 44<br />
D 134<br />
D 183<br />
D 360<br />
D 1470<br />
In Figure 4 there are typical<br />
dependencies <strong>of</strong> the pyrene and perylene<br />
<strong>in</strong> hydrophobized hyaluronan solution.<br />
Pyrene ratio shows typical decreas<strong>in</strong>g Stype<br />
curve. Perylene, on the other hand,<br />
shows two l<strong>in</strong>e system. For pyrene it was<br />
used equations 1) and 2) to solve<br />
parameter cac1 and cac2, respectively.<br />
Perylene’s data, cacPe was solved by<br />
comb<strong>in</strong>ation <strong>of</strong> two l<strong>in</strong>ear equations as<br />
po<strong>in</strong>t <strong>of</strong> <strong>in</strong>tersection. F<strong>in</strong>al values (<strong>in</strong><br />
g·l -1 ) are: cac1 = 0.179, cacPe = 0.178,<br />
cac2 = 0.758. So it was established the<br />
best value for the cac determ<strong>in</strong>ation is<br />
cac1 po<strong>in</strong>t, realized as the <strong>in</strong>flex po<strong>in</strong>t x0.<br />
The cac values are show<strong>in</strong>g two<br />
trends. First, the cac values are<br />
decreas<strong>in</strong>g with <strong>in</strong>creas<strong>in</strong>g SD (except for<br />
samples D 1470). Second, the cac values<br />
are decreas<strong>in</strong>g with <strong>in</strong>creas<strong>in</strong>g M. These trends are obvious <strong>in</strong> Figure 5 and summarized <strong>in</strong><br />
Table 1. The greatest decreas<strong>in</strong>g <strong>of</strong> the cac values show D 44 samples. It is possible to relate<br />
this behavior to the fact that D 44 samples have the shortest cha<strong>in</strong>s and new types <strong>of</strong><br />
<strong>in</strong>teractions can be found<strong>in</strong>g. On the other hand, heavy weighted cha<strong>in</strong>s show stable behavior<br />
aga<strong>in</strong>st the SD changes. These results can be expla<strong>in</strong>ed if we accept that next addition <strong>of</strong> the<br />
alkyls to the cha<strong>in</strong> only fortify cha<strong>in</strong>-cha<strong>in</strong> <strong>in</strong>teraction. And <strong>in</strong> fact, it does not lead to the<br />
formation <strong>of</strong> new hydrophobic cores.<br />
cac (10 -6 mol l -1 )<br />
100.00<br />
10.00<br />
1.00<br />
0.10<br />
0.01<br />
10<br />
30<br />
50<br />
10<br />
30<br />
50<br />
70<br />
10<br />
30<br />
50<br />
10<br />
30<br />
50<br />
30<br />
50<br />
70<br />
12.27<br />
1.02<br />
0.06<br />
4.35<br />
1.21<br />
0.76<br />
0.20<br />
0.99<br />
0.73<br />
0.47<br />
0.51<br />
0.19<br />
0.18<br />
0.15<br />
0.06<br />
0.10<br />
D 44 D 134<br />
D 183 D 360<br />
D 1,470<br />
0 20 40 60 80<br />
SD (%)<br />
Figure 5 The dependences <strong>of</strong> the cac values on the SD. Except for D 1470 all samples<br />
show the decreas<strong>in</strong>g tendencies with <strong>in</strong>creas<strong>in</strong>g M and SD.<br />
CONCLUSION<br />
Fluorescence determ<strong>in</strong>ation <strong>of</strong> cac for the novel hyaluronate derivatives was presented. It<br />
was showed cac1 as the best po<strong>in</strong>t for determ<strong>in</strong>ation <strong>of</strong> the critical aggregation concentration.<br />
Sborník soutěže Studentské tvůrčí č<strong>in</strong>nosti Student 2006 a doktorské soutěže O cenu děkana 2005 a 2006<br />
Sekce DSP 2006, strana 216