production of selected secondary metabolites in transformed ...

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Determination of fatty acids A simplified method for analysis of fatty acids in human serum was used according to Kang at al. 2005 (4). Fatty acids were methylated using BF3/methanol reagent and extracted to hexane phase. Fatty acids methyl esters were measured using GC-FID method. In each sample 30 different fatty acids were detected using external standards. RESULTS After 3-month intake OF PUFA/tocopherol supplemet a significant decrease of serum cholesterol, LDL-cholesterol (10-15%) and mainly triglycerides (about 35%) in hyperlipidaemic patients was observed. No similar changes in controls was shown. While the decrease of cholesterol as well as LDL-cholesterol levels was caused predominantly by tocopherol effect, TAG levels could be influenced by combined effect of PUFA and tocopherol. Further, PUFA/tocopherol intake led to a significant increase of tocopherol levels and TAS and, thus, to corresponding decrease of serum AGEs and AOPPs. The profiles of serum fatty acids (FA) shown also some changes after supplementation. A significant decrease of saturated FA (myristic, palmitic, stearic acid) was observed in all groups. Different changes of unsaturated FA were observed in hyperlipidaemics when compared with controls. A significant increase of PUFA mixture, EPA and DHA was found in controls, while no changes of PUFA, EPA and moderate changes of DHA were observed in hyperlipidaemics. The main problem with any epidemiological study is that correlation does not imply causation. There are many other factors, that could be responsible for biological effect. Additional problems are connected with group composition as well as with biomarker selection (1). Moreover, in Czech population basal levels of antioxidants were changing in the course of time. Despite these problems, our results indicated, that intake of food supplement containing PUFA and tocopherol can positively influence lipid metabolism and antioxidant status in patients with hyperlipidaemia. Very important is composition of vitamin preparative; many commercial PUFA are extensively oxidized and, thus, isoprostan formation can occur. Acknowledgements: This work was supported by project FRVS 3150/G1/2006 the Czech Ministry of Education, Youth and Sport. References: 1. Halliwell B., Gutterdige J.M.C.: Free Radicals in Biology and Medicine. 3rd Edition, Oxford University Press, 1999 2. Witko-Sarsat et al.: Advanced Oxidation Protein Products as Novel Mediators of Inflammation and Monocyte Activation in Chronic Renal Failure. The Journal of Immunology 2524-2532, 1998 3. Kalousová M. et al.: Advanced Glycation End-Products and Advanced Oxidation Protein Products in Patients with Diabetes Mellitus. Physiol. Res. 51: 597-604, 2002 4. Kang J.X., Wang J.: A simplified method for analysis of polyunsaturated fatty acids. BMC Biochemistry 6:5, 2005 Sborník soutěže Studentské tvůrčí činnosti Student 2006 a doktorské soutěže O cenu děkana 2005 a 2006 Sekce DSP 2006, strana 212
HYDROPHOBIZED SODIUM HYALURONATE IN AQUEOUS SOLUTION - A FLUORESCENCE STUDY Ing. Filip Mravec, 3 rd year PGS Supervisor: doc. Ing. Miloslav Pekař, CSc. Brno University of Technology, Faculty of Chemistry, Institute of Physical and Applied Chemistry, Purkyňova 118, 612 00 Brno, e-mail: mravec@fch.vutbr.cz INTRODUCTION Polysaccharides and their derivatives have become as major components for the development of biocompatible and biodegradable materials with many areas of interests (e.g. tissue engineering, drug delivery). Chemical modification, which no affected biodegradability, can lead to the expansions of medicine and engineering applications. Hyaluronan is major component of pericellular and extracellurar matrices. It is a linear polymer of the disaccharide D-glucuronic acid-1-β-3-N-acetylglukosamine (Figure 1a). It plays important role in stabilizing the extracellular matrix in many tissues by binding to specific proteins called hyaladherines. The main hyaluronan fraction is localized in skin tissue. The preparing of the hyaluronan derivatives are generally based on the esterifcation on the D-glucuronic subunit. Our derivatives were modified on the second carbon on the glucuronic subunit (Figure 1b). Because carboxylic groups are still free, we obtained the amphiphilic polyelectrolyte - hydrophobized hyaluronan (hHA). From its structure we predict in aqueous solution modified hyaluronan will aggregate to form micelle-like structures with non-polar core. This aggregation behavior can be study by non-polar fluorescence probes solubilized to H H O O HO a O O HO b O O - O - O OH O Na + Na + O NH R HO O HO O C H 3 C H 3 Figure 1 Structure of the native hyaluronan (a) and its hydrophobized derivative (b), R = C10. this core. Pyrene, benzo[d,e,f]fenanthrene is the even and alternating hydrocarbon (Figure 2a). The “Pyrene I1:I3 ratio method” is widely used method to determine the critical aggregation concentration (cac) for a lot of surfactant-based systems. Its unique response to the Sborník soutěže Studentské tvůrčí činnosti Student 2006 a doktorské soutěže O cenu děkana 2005 a 2006 Sekce DSP 2006, strana 213 OH OH O NH O O NH O n n OH OH
Page 1 and 2: PRODUCTION OF SELECTED SECONDARY ME
Page 3 and 4: containing ampicillin, IPTG and x-g
Page 5 and 6: THE SIMPLE METHOD FOR THE RECOGNITI
Page 7 and 8: Figure 1 Negative-ion mode MALDI-TO
Page 9 and 10: Table 1 Evaluation of negative-ion
Page 11 and 12: Fig. 1: Scheme of the hydride gener
Page 13 and 14: [4] H. Matusiewicz, M. Kopras, J. A
Page 15: - are hypotriglyceridemic; - exhibi
Page 19 and 20: spectrophotometer (AMINCO-Bowman, S
Page 21 and 22: Hyaluronate derivatives showed with
Page 23 and 24: copolymers were additionally functi
Page 25 and 26: Each micelle has a hydrophobic core
Page 27 and 28: STUDY OF THE COPPER(II) IONS NON-ST
Page 29 and 30: CuSO4, Cu(ClO4)2 and Cu2P2O7 of the
Page 31 and 32: total flux [mol.m -2 ] 0.7 0.65 0.6
Page 33 and 34: number of free radicals is very low
Page 35 and 36: Differences can be caused by severa
Page 37 and 38: MEASUREMENT OF THERMOPHYSICAL PROPE
Page 39 and 40: The typical time responses of tempe
Page 41 and 42: Figure 4 illustrates the typical de
Page 43 and 44: sodium sulphate and filtered to a 5
Page 45 and 46: Compare all tested evening primrose
Page 47 and 48: IMPROVED FLUORIMETRIC DETERMINATION
Page 49 and 50: Al F i 80 70 60 50 40 30 20 10 0 0
Page 51 and 52: F i 60 50 40 30 20 10 Data UCL LCL
Page 53 and 54: PHYSICAL-CHEMICAL ASPECTS OF PAINT
Page 55 and 56: Finally, the solubility parameter o
Page 57 and 58: Study of the influence of different
Page 59 and 60: Fig.1: Chemical structures of pI ma
Page 61 and 62: Identification of pI markers - Firs

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