CHL2008 Book of abstract - Fakulta chemická - Vysoké učení ...

Brno University of Technology 

Faculty of Chemistry 

4 th Meeting 

on Chemistry and Life 

Book of Abstracts 

Brno, September 9–11, 2008

Editor’s note: 

The contributions published in this Book of Abstracts as well as the full text papers 

published in a special issue of the Chemické listy journal share the same numbering 

scheme based on alphabetical order of corresponding author surname within the individual 

conference sections. Author index is available at the Appendices chapter of 

this Book. Based on the requests of some authors, several full text contributions were 

not published in the journal even though they had been presented at the conference 

(either in the form of posters or lectures). Short abstracts of all the conference contributions 

are published in this Book of Abstracts of the 4 th Chemistry and Life 2008 

conference and are also available at the conference website 

(www.fch.vutbr.cz/chl_2008). 

© Petr Dzik (ed.), 2008 

ISBN 978-80-214-3715-9

Contents 

Preface 

Conference Partners ............................................................................................................43 

Foreword ...............................................................................................................................45 

Plenary Lectures 

PL-01 Functional Dairy Products – from Traditional to Modern 

Technology ..............................................................................................................P.3 

Irena Rogelj and Bojana Bogovič Matijašić 

PL-02 Alkali-activated Aluminosilicates: Past, Present and Future ...........................P.4 

Pavel Krivenko 

PL-03 Organic Anthropogenic Contaminants in River Systems 

– Complementary Environmental Approaches .................................................P.5 

Jan Schwarzbauer 

PL-04 Dark Side of Chemistry in Human Civilization ................................................P.6 

Pavel Častulík 

PL-05 Challenges of the Analysis of Food and Environmental Matrices 

by GC-TOF MS and GCxGC-TOF MS ................................................................P.7 

Tomáš Kovalczuk, Jitka Zrostlíková, Jana Hajšlová, Jakub Schůrek, Mark 

Libardoni 

PL-06 Nanoparticle Characterisation Using Light Scattering Techniques ...............P.8 

Michael Kaszuba 

PL-07 Rapid stability assessment and particle characterization for food 

and non-food products by multisample analytical centrifugation .................P.9 

D. Lerche, T. Sobisch, S. Küchler, A. Uhl 

PL-08 Transformation of Petroleum Type Pollutant in the Environment ...............P.10 

Branimir Jovancicevic

Table of Content 

Section 1: Environmental Chemistry & Technology 

Lectures 

1-L01 Ecotoxicity vs. Contamination vs. Composition of River 

Sediments: the Quest for Determinants of Toxic Effects ..................................1.5 

Luděk Bláha, Klára Hilscherová, Tomáš Čáp, Jana Klánová, Jiří Machát, 

Ivan Holoubek 

1-L02 Degradation Products of Synthetic Polymers as Emerging 

Environmental Contaminants ..............................................................................1.6 

Josef Čáslavský, Milada Vávrová, Daniela Mácová, Ludmila Mravcová 

1-L03 Usage of Gas Chromatography and IMS Detection for Evaluation 

of Polymer Barrier Material Properties ...............................................................1.7 

J. Dvořáková, I. Mašek 

1-L04 Complex Essessment of Organophosphates Low Dose Chronic 

Exposure on Endothelium, Macrophages, Platelets and Esterases ...............1.8 

Ermolaeva E.,Goncharov N., Radilov A., Glashkina L., Mindukshev I., 

Avdonin P., Dobrylko I., Rembovskiy V. 

1-L05 Influence of Climate Conditions and Air Contamination on 

Village Inhabitants Health ....................................................................................1.9 

Melánia Feszterová, Slavomíra Kašiarová 

1-L06 Integration Metallomics, Proteomics and Transcriptomics in 

Environmental Issues ..........................................................................................1.10 

J. L. Gomez-Ariza, T. Garcia-Barrera, M. Gonzalez-Fernandez, J. Lopez- 

Barea and C. Pueyo * 

1-L07 Heavy Metals in Solid Immisions in the Vicinity of Iron Ore 

Mining and Processing Plant in Nižná Slaná ...................................................1.11 

J. Hančuľák, E. Fedorová, O. Šestinová, T. Špaldon, P. Slančo, J. Brehuv 

1-L08 Comparison of Energy Dispersive X-RAY Fluorescence 

Spectrometry, Inductively Coupled Plasma Optical Emission 

Spectrometry and Laser Ablation with Plasma Spectrometry 

and Laser Ablation with Plasma Spectrometry in the Elemental 

Analysis of Experimenta .....................................................................................1.12 

Ivona Hubová 

1-L09 Tissue-Specific Distribution and Accumulation of Organochlorine 

Pollutants in Selected Raptor Species from the Czech Republic ..................1.13 

Radim Lána, Milada Vávrová, Vladimír Večerek, Stanislav Kráčmar


1-L10 CBRN Terrorism and International Legal Constraints ...................................1.14 

Jiří Matoušek 

1-L10 Optimization of Combustion Process in a Biomass Fuel Bed as a 

Way to Reduce a Greenhouse Gas Emitions ........................................................... 

Biljana Miljkovic, Borivoj Stepanov, Ivan Pesenjanski 

1-L11 Applicability of a High Performance Chelation Ion 

Chromatographic Method to the Determination of Aluminium in 

Antarctic Surface Seawater .................................................................................1.15 

Juliette Tria, Pavel Nesterenko, Andrew Bowie, Edward Butler, Paul Haddad 

1-L12 Direct Analysis in Real Time–Time-of-Flight Mass Spectrometry: 

Analysis of Pesticides and Enviromental Contaminans .................................1.16 

Lukáš Václavík, Jakub Schůrek, Tomáš Čajka, Jana Hajšlová 

1-L13 The Using of Pine-Needles, Bryophytes and Sponges as 

Bioindicators for the Evaluation of Persistent Organic Pollutants 

Environmental Levels ..........................................................................................1.17 

M. Vávrová, R. Lána, M. Hroch, I. Hlaváčková, J. Čáslavský, B. Tremlová 

Posters 

1-P01 Ultra Trace Determination of Silver in Preconcentrated Water 

Samples by Electrothermal Atomic Absorption Spectrometry .....................1.21 

Seyyed Hamid Ahmadi, Javad Didevar Asl 

1-P02 Removal of 2-mercaptobenzothiazole from Synthetic Wastewater ..............1.22 

Beáta Almásiová, Ján Derco, Angelika Kassai 

1-P03 Health Risk Assessment by Indoor Air Quality Monitoring .........................1.23 

Ekaterina Andreeva, Ivan Mašek, Milada Vávrová 

1-P04 Nitrogen in breeding laying hens and environment protection ...................1.24 

Mária Angelovičová, Marek Angelovič, Miroslava Kačániová, Daniela 

Liptaiová, Kamil Močár, Dávid Štofan 

1-P05 Testing of Various Sorbents for Copper Removal from Acid Mine 

Drainage ................................................................................................................1.25 

Magdaléna Bálintová, Natália Kovaliková 

1-P06 Influence of Composite Sorbent for Selected Elements Content in 

the Sediment Load of a Water Reservoir and Mine Sludge Basin.................1.26 

Ján Brehuv, Oľga Šestinová, Tomislav Špaldon, Pavel Slančo, Jozef 

Hančuľák, Erika Fedorová


1-P07 Monitoring of Different Trace Metals Species Concentration in 

Morava River ........................................................................................................1.27 

Lukáš Brulík, Pavel Diviš, Hana Dočekalová and Jiří Machát 

1-P08 Volatile Degradation Products of Polyurethane Foams .................................1.28 

Josef Čáslavský, Daniela Mácová, Tereza Tobiášová 

1-P09 English fro Chemists Can Be pHun ...................................................................1.29 

Gabriela Clemensová 

1-P10 Dynamic Simulation of Biological Nitrogen Removal Processes....................1.30 

Lenka Černochová, Ján Derco 

1-P11 Application of a 6-Mercaptopurine Functionalised Sorbent for 

Diffusive Gradients in Films Technique ...........................................................1.31 

Pavel Diviš and Roman Szkandera 

1-P12 The Determination of Methylmercury in Water Ecosystems ........................1.32 

H. Doležalová Weissmannová, J. Čáslavský, M. Vávrová, L. Tuhovčáková 

1-P13 Ecotoxicological Testing and Test Methods of Chemicals and 

Waste ......................................................................................................................1.33 

H. Doležalová Weissmannová, H. Zlámalová Gargošová 

1-P14 Examining Indoor Aerosols ................................................................................1.34 

Adriana Eštoková, Nadežda Števulová, Lenka Kubincová 

1-P15 Atmospherical Deposition and Imission Situation in the Nižná 

Slaná Area ..............................................................................................................1.35 

Erika Fedorová, Jozef Hančuľák, Oľga Šestinová, Ján Brehuv, Tomislav 

Špaldon 

1-P16 The Natural and Anthropological Sources of the Halciansky 

Water Reservoir Contamination ........................................................................1.36 


1-P17 Biomodified Forms of Zeolites and their Environmental 

Application .........................................................................................................1.37 

Ľubica Fortunová, Mária Reháková, Silvia Čuvanová, Lucia Gaberová, 

Mária Kušnierová


1-P18 Distribution Patterns of Organic Pollutants in Brno Lake with 

Respect to Its Depositional History ..................................................................1.38 

Eva Francu, Jan Schwarzbauer, Mathias Ricking, Radim Lána, Pavel 

Muller, Juraj Francu 

1-P19 New Resin Gel for Diffusive Gradients in Thin Film (DGT) 

Technique ..............................................................................................................1.39 

M. Gregušová, B. Dočekal, H. Dočekalová 

1-P20 Extending of the Scope of GC/MS Method for Multiple pesticide 

Residues: Validation Study ................................................................................1.40 

Markéta Hakenová, Jana Urbanová, Ondřej Lacina, Vladimír Kocourek, Jana 

Hajšlová 

1-P21 Challenges in the Analysis of Hexabromocyclododecane (HBCD) 

in the Environmental Samples ...........................................................................1.41 

Petra Hrádková, Jan Poustka, Jana Pulkrabová, Michaela Nápravníková, 

Jana Hajšlová 

1-P22 The Content of Polybrominated Diphenyl Ethers in Freshwater 

Fishes from Brno Water Reservoir .....................................................................1.42 

M. Hroch , M. Vávrová , V. Večerek 

1-P23 Examination of Aband)oned Sb Deposits by Mineralogical and 

Geochemical Methods in Poproč (Slovakia) .....................................................1.43 

Michal Jankulár, Tomáš Klimko, Michal Šutriepka, Marek Vaculík 

1-P24 Determination of Volatile Compounds and Saccharides at Alder 

Wood Hydrolysis .................................................................................................1.44 

František Kačík, Marta Laurová, Danica Kačíková 

1-P25 Determination of Methylmercury in Sediments by Headspace 

Gas Chromatography and Atomic Fluorescence Detection after 

Aqueous Phase Ethylation with Sodium Tetraethylborate ............................1.45 

Milada Kadlecová, Baghdad Ouddane, Hana Dočekalová, Jean-Claude 

Fischer 

1-P26 Modeling of Dispersion of Windborne Material in Atmosphere ..................1.46 

Michal Kapoun, Radim Dvořák 

1-P27 Reprocessing of Dangerous put out Chemicals and Wastes ........................1.47 

Juraj Kizlink


1-P28 Application of Chitosan by Water Treatment .................................................1.48 

Zuzana Klímová 

1-P29 Substituted Carboxylic Acids as a Guest for Cucurbiturils ...........................1.49 

Viktor Kolman, Vladimír Šindelář 

1-P30 The Effect of Waste Basalt Wools on the Chemical, Agrochemical, 

Pedological and Hygienic-Toxicological Soil Parameters ..............................1.50 

Peter Kováčik, Alena Vollmannová, Jaroslav Noskovič 

1-P31 Influence of Water Erosion Processes on the Bottom Sediment 

Quality ...................................................................................................................1.51 

Natália Kovaliková, Magdaléna Bálintová 

1-P32 Modelling And Diagnosing of Mechanical Engineering Life Cycle 

Production Process ..............................................................................................1.52 

Ružena Králiková, Alena Paulíková 

1-P33 Simulation Of Chemical Factors in Working Environment ..........................1.53 

Ružena Králiková, Alena Pauliková 

1-P34 Multi-Residue Method for the Analysis of Pesticides and 

Mycotoxins in Cereals by LC-MS/MS ...............................................................1.54 

Ondřej Lacina, Jana Urbanová, Alexandra Krplová, Lukáš Václavík, Jana 

Hajšlová and Jan Poustka 

1-P35 Aerobic MTBE Biodegradation by Paecilomyces Variotii ............................1.55 

Blazo Lalevic,Vera Raicevic, Ljubinko Jovanovic, Dragan Kikovic,Miomir 

Niksic 

1-P36 Reclamation of Calcium Sulphate Sludges by Sulphate-Reducing 

Bacteria .................................................................................................................1.56 

Alena Luptáková, Mária Kušnierová 

1-P37 Bioleaching of Antimony Mineralsby Bacteria Acidithiobacillus 

and Desulfovibrio ...............................................................................................1.57 

Alena Luptáková, Eva Mačingová, Jana Jenčárová 

1-P38 Biological-Chemical regeneration of Desulphurization Sorbents 

Based on Zinc Ferrite ...........................................................................................1.58 

Alena Luptáková, Mária Kušnierová, Vladimír Šepelák


1-P39 Single Analyte Zone Splitting in CZE: Origin, Appearance and 

Remedy ..................................................................................................................1.59 

Z. Malá, P. Gebauer, P. Boček 

1-P40 Chlorophyll Bleaching by UV-Irradiation in Solvents and Isolated 

Photosynthetic Organelles ..................................................................................1.60 

Dejan Marković, Jelena Zvezdanović 

1-P41 Environmental Risk Assessment, Transport of Dangerous Goods 

and Possible Accidents ........................................................................................1.61 

Jana Victoria Martincová and Ivan Mašek 

1-P42 Causes of Accidents Regarging Transport of Dangerous Goods .................1.62 

J. V. Martincová, I. Mašek, J. Martinec 

1-P43 Development of a new Educational Programme “Protection of 

Population” ...........................................................................................................1.63 

Ivan Mašek, Pavel Častulík, , Otakar Mika 

1-P44 Can we Ensure Safer Environmental for Curtural Heritage? ........................1.64 

Ivan Mašek, Zdena Rosická 

1-P45 Risks Factors of Social Development for Management .................................1.65 

Ivan Mašek, Jaromír Novák 

1-P46 Actual Problems of chemical Disarmanet: Chemical Weapons 

Convention after the 2nd Review Conference .................................................1.66 


1-P47 Protection of Population in The Czech Republic and its 

Perspectives ...........................................................................................................1.67 

Otakar J. Mika, Lenka Fišerová 

1-P48 Application of DGT Method for Assesment of Availability of 

Heavy Metals to Plants .......................................................................................1.68 

Z. Mládková, K. Pešková, B. Dočekal, H. Dočekalová, P. Škarpa 

1-P49 Spectrophotometric Microdetermination of Phosphate Based on 

the Ion Association Complex with Rhodamine B in Water ...........................1.69 

Martin Moos, Lumír Sommer 

1-P50 Determination of Uranium by ICP-AES in the Absence and 

Presence of Preconcentration on Macroporous Sorbents ..............................1.70 

Martin Moos, Urbánková Kristýna, Lumír Sommer


10 

1-P51 Determination of halogenated Pollutants in Environmental 

Samples by using GC-ECD after SPME-GC Separation ...............................1.71 

F. Moreno, J. M. Manso, T. García-Barrera, J. L. Gómez-Ariza 

1-P52 Simultaneous Speciation of Selenium and Mercury in Biological 

Samples by using a Column Swithching Systém with Liquid 

Chromatography Coupled to ICP-MS .............................................................1.72 

F. Moreno, T. García-Barrera, J. L. Gómez-Ariza 

1-P53 Volatile Organic Substances Present in Spices and Spruce Needles ............1.73 

Ludmila Mravcová, Milada Vávrová, Josef Čáslavský, Michaela Stoupalová, 

Ilona Hlaváčková, Hana Vítečková 

1-P54 Changes in Carotenoids Pattern in Mougeotia sp. Algae Induced 

By High Light Stress ............................................................................................1.74 

Edward Muntean, Victor Bercea, Nicoleta Muntean, Nicolae Dragoş 

1-P55 Simultaneous Ion Chromatographic Determination of Anions and 

Cations in Surface Waters From Fizes Valley ...................................................1.75 

Muntean Edward, Mihăiescu Tania, Muntean Nicoleta, Mihăiescu Radu 

1-P56 Strategies to Reduce Detection Limits (LODs) in the Analysis of 

Brominated Flame Retardants (BFRs) in Environmental Samples ...............1.76 

Michaela Nápravníková, Jana PulkrabováPetra Hrádková, Jana Hajšlová, 

Jan Poustka, Dita Svobodová 

1-P57 Chemicals and Energy Form Biomass ..............................................................1.77 

Ľudovít Polívka, Eva Ürgeová 

1-P58 Simultaneous Determination of Mercury, Lead and Cadmium in 

Aqueous Samples Using Picric Acid-Modified Carbon Paste Electrode .....1.78 

Jose H. Santos, Sean Gerard Ward 

1-P59 Detection of Foreign Organic Substances in Water and Biological 

samples ..................................................................................................................1.79 

E. Savelieva, N. Goncharov, N. Koryagina, N. Khlebnikova, A. Radilov 

1-P60 Determination of Sulfonamides in water using Multiwalled 

Carbon Nanotubes Solid Phase Extraction and HPLC with 

Fluorescence Detector .........................................................................................1.80 

Stefania Simon, Dan Lupu, Alexandru R. Biris , Constantin Bele 

1-P61 Microbiologial Remediation of Metal-Contaminated Soil .............................1.81 

Valéria Snopková, Katarína Jablonovská


1-P62 Distribution of Phthalic Acid Esters (DEPH, DBS) in Chicken 

Tissues and Organs ..............................................................................................1.82 

Vlasta Stancová, Lenka Krátká, Alžběta Jarošová, Jiří Harazim, Pavel Suchý 

1-P63 Purification Process Influence on the PAH Determination in Real 

Soil Samples ..........................................................................................................1.83 

M. Stoupalová, M. Vávrová, L. Mravcová, V. Večerek 

1-P64 The Optimalisation of SPME Method for Determination of 

Essential Oils in Spice ..........................................................................................1.84 

M. Stoupalová, H. Pleskačová, M. Vávrová, L. Mravcová, V. Večerek 

1-P65 DTA and Fluorescence Spectra of Humic Acids as Indicators of 

Human Influence on Soil ....................................................................................1.85 

Nora Szombathová, Jörg Luster, Božena Debska, Anton Zaujec,Milan 

Macák, Erika Tobiašová, Juraj Chlpík, Vladimír Šimanský 

1-P66 Evaluation of Heavy Metals Mobility in the Sediments from the 

Hnilec River, Slovakia ..........................................................................................1.86 

Oľga Šestinová, Ján Brehuv, Jozef Hančuľák ,Tomislav Špaldon, Erika 

Fedorová 

1-P67 Sulphates Reduction from Waste Water of Old Mine Loads .........................1.87 

T. Špaldon, J. Brehuv, J. Hančuľák, E. Fedorová, O. Šestinová 

1-P68 Arsenic Removal From Water by Synthetic Akaganeite ................................1.88 

Katarína Štefušová, Miroslava Václavíková, George P. Gallios, Štefan 

Jakabský 

1-P69 The EPR Study of Particulate Matter .................................................................1.89 

Nadežda Števulová, Adriana Eštoková, Pavel Stopka 

1-P70 Selection of Packing Materials for Biofilter Development .............................1.90 

Iveta Štyriaková, Alexandra Vašková 

1-P71 Antibiotic Effects Of The Naphtoquinonic Derivatives On Gram- 

Positive And Gram-Negative Germs ................................................................1.91 

Radu Tamaian, Nadia Păun, Andreea Iordache, Raluca Vremeră, Ştefania 

Broscăţean 

1-P72 Practical Application in Agriculture of the Magnesium Products 

Industry Waste .....................................................................................................1.92 

Lidia Taubert, Hortensia Rădulescu, S. A. Kiss, R. Kastori, Ecaterina 

Princz, Éva Stefanovits-Bányai 

11


12 

1-P73 Possibilities of Objective Odorisation Control of Natural Gas ......................1.93 

Daniel Tenkrát, Ondřej Prokeš, František Humhal 

1-P74 Contamination of Soil and Alimentary wheat in Zemplínska 

Polluted Area ........................................................................................................1.94 

Ján Tomáš, Juraj Čéry, Ladislav Lahučký, Janette Musilová 

1-P75 Determination of Changes in Soil Organic Matter Content through 

Carbon and Nitrogen Labile Fractions ..............................................................1.95 

Tomáš Tóth, Erika Tobiašová, Vladimír Šimanský 

1-P76 Distribution of Heavy Metals in Soil .................................................................1.96 

Tomáš Tóth, Alena Vollmannová, Tomáš Ján, Peter Lazor 

1-P77 Separation and Preconcentration of Arsenic, Antimony, Selenium 

and Tellurium on Modified Silicagels for their Determination by 

ICP-AES .................................................................................................................1.97 

Kristýna Urbánková, Lumír Sommer, Martin Moos 

1-P78 Multicomponent Microdetermination of Arsenic, Antimony, 

Tellurium, Selenium Besides of Thallium by ICP-MS in Waters ...................1.98 

Kristýna Urbánková, Jiří Machát, Lumír Sommer 

1-P79 Treating Wastewater by Using of Bioceamic Filters .......................................1.99 

A. Vaskova, I. Styriakova, D. Kupka, V. Snopkova 

1-P80 Selected MUSK Compounds in Abiotic and Biotic Samples .......................1.100 

M. Vávrová, D. Štenclová, E. Blahová, J. Mareš, M. Mášová, J. Čáslavský 

1-P81 Determination of Surfactants Included in Sewage Water ...........................1.101 

Milada Vávrová, Lenka Langová, Helena Zlámalová Gargošová, Kristýna 

Kubíčková, Vladimír Večerek 

1-P82 Examination of the Mutual Interaction of Heavy Metals in Course 

of Adsorption from Model Solutions ..............................................................1.102 

Ján Vereš, Tomáš Bakalár, Milan Búgel, Martin Sisol 

1-P83 Fungicidal Effect of Printed Titanium Dioxide Layers .................................1.103 

Mária Veselá, Michal Veselý, Petr Dzik,Jana Chomoucká, Lenka Šupinová 

1-P84 Photocatalytic Disinfection of Water Using Ag/TiO 2 ....................................1.104 

Mária Veselá, Michal Veselý, Jana Chomoucká, Michaela Lipenská


1-P85 Laboratory Study of Arsenic Mobility in Stream Sediments and 

Impoundment Material using Column Experiments ..................................1.105 

Veronika Veselská, Edgar Hiller 

1-P86 Antibiotics in the Environment ..........................................................................1.106 

H. Vítečková, L. Vydrová, M. Vávrová, L. Mravcová 

1-P87 The Fluorimetric Determination of Aluminium, Gallium and 

Indium with 8-Hydroxyquinoline-5-sulphonic acid in Aqueous 

and Submicellar Medium ..................................................................................1.107 

Šimon Vojta, Lumír Sommer 

1-P88 Surgical Polyester Fabric Impregnated by Cross-Linked Collagen ............1.108 

Lucy Vojtová,Pavel Filka, Josef Jančář 

1-P89 Soil Hygiene in Old Environmental Burden Areas .......................................1.109 

Alena Vollmannová, Ján Tomáš, Daniel Bajčan, Peter Kováčik 

1-P90 EPR Study on Photoinduced Processes of Novel Quinolone 

Derivatives ..........................................................................................................1.110 

Zuzana Vrecková, Vlasta Brezová, Maroš Bella, Viktor Milata 

1-P91 The Boron in Kraft Pulp Mill and Influence in Waste Water.................... 1.111 

Milan Vrška, Eva Gemzicka, 

1-P92 Contents of Different Fractions of Sulphur in Slovakia Soils ......................1.112 

Anton Zaujec, Lýdia Jedlovská, Melánia Feszterová, 

1-P93 Factors Influencing the Sorption Behaviour of Herbicide 

Acetochlor in Soils and Sediments .................................................................1.113 

Lenka Zemanová, Edgar Hiller, Zoltán Krascsenits 

1-P94 Ecotoxicological Evaluation of the Sludges From Waste Water 

Treatment Plants .................................................................................................1.114 

Helena Zlámalová Gargošová, Lucie Hellingerová,Milada Vávrová 

1-P95 Microwave Desulphurization Of Coal ............................................................1.115 

Ingrid Znamenáčová, Michal Lovás, Silvia Čuvanová, Štefan Jakabský 

1-P96 The Biological Activity of Tetracyclic Diterpene Isolated from The 

Slovak Brown Coal .............................................................................................1.116 

Anton Zubrik, Andrea Lauková, Alena Gábelová, Zuzana Valovičová, Josef 

Cvačka, Ľudmila Turčániová 

1


Section 2: Food Chemistry & Biotechnology 

Lectures 

2-L01 Nonsaccharomyces Yeast in Grape Must – Advantege or Spoilage? .............2.5 

Emília Breierová, Jaroslava Kaňuchová Pátková, Ingrid Vajciková, 

1 

2-L02 Approaches to Minimization of Acrylamide Level in Foods...........................2.6 

Zuzana Ciesarová 

2L-03 Solid State Fermentation as a Tool for Preparation of Bioproducts 

Enriched with Polyunsaturated Fatty Acids ......................................................2.7 

Milan Čertík, Zuzana Adamechová, Linda Németh 

2-L04 Emerging Technologies in Food Analysis .........................................................2.8 


2L-05 Physiological Regulation of Biotechnological Production of 

Carotenoid Pigments .............................................................................................2.9 

Vladimíra Hanusová, Martina Čarnecká, Andrea Halienová, Milan Čertík, 

Emília Breierová, Ivana Márová 

2-L06 Enzymatic Saccharide Acetylations in Water – Comparation of 

Catalysts ................................................................................................................2.10 

Vladimír Mastihuba, Tatiana Kraková, Mária Mastihubová 

2-L07 Preparation of Bio-Diesel from Tall Oil ............................................................2.11 

Radoslav Mikulášik, Ján Cvengroš, Viktor Milata, Martin Polovka, Svetozár 

Katuščák, Igor Šurina 

2-L08 Artificial Neural Networks in Food Analysis ..................................................2.12 

Ján Mocák, Viera Mrázová, Dáša Kružlicová, Filip Kraic 

2-L09 Detection of Spices‘ Irradiation by Modern Spectroscopic 

Techniques .............................................................................................................2.13 

Martin Polovka, Milan Suhaj 

2-L10 Application Potential of Novel GC-HT TOF MS (TRU TOF) 

System in Food and Environmental Analysis .................................................2.14 

Jakub Schurek, Jana Pulkrabova, Jana Hajslova 

2-L11 Cofffe as a Source of Antioxidants (An EPR Study) ........................................2.15 

Andrej Staško, Vlasta Brezová and Anna Šlebodová


2-L12 Interactions Between Organic Food Contaminants and Plastic 

Packaging Materials ............................................................................................2.16 

Peter Šimko, Božena Skláršová, Peter Šimon, B. Drobná 

2-L13 Analysis of Fusarium Mycotoxins, Critical Assessment ...............................2.17 

Zachariasova Milena, Hajslova Jana, Poustka Jan, Kostelanska Marta, 

Krplova Alexandra, Vaclavik Lukas 

Posters 

2-P001 Determination of β-Carotene in the Gelatin Capsule .....................................2.21 

B. Altangerel, Z. Kolajova, D. Kramarova, O. Rop, I. Hoza 

2-P002 Determination of β-Carotene in Tomato by High Performance 

Liquid Chromatography with Electrochemical Detector ...............................2.22 

B. Altangerel, P. Vojtiskova, D. Kramarova, , O. Rop, I. Hoza 

2-P003 Protective Effect of Tomatoes Powder in Oxidative Stress Induced 

by Thyroid Disfunctions ....................................................................................2.23 

Sanda Andrei, Adela Joanta, Adela Pintea, M. Merca Sarlea 

2-P004 Thermophilic Bacteria Application to Whey Biodegradation .......................2.24 

Libor Babák 

2-P005 Growth Curves of Mixed Thermophilic Bacteria ............................................2.25 

Libor Babák, Radka Burdychová, Vlastimil Dohnal 

2-P006 Monitoring of Acrylamide Levels Using LC-MS/MS: Malts and 

Beers .....................................................................................................................2.26 

Veronika Bartackova, Lukas Vaclavik, Katerina Riddellova,Jana Hajslova 

2-P007 Determination of Polycyclic Aromatic Hydrocarbons in Czech 

Honeys ...................................................................................................................2.27 

Petra Batelková, Ivana Borkovcová,Klára Bartáková, Lenka Vorlová 

2-P008 Quantitative Determination of Sulfonamide Residues in Chicken 

Meat by a New Solid Phase Extraction and HPLC-UV for Detection .........2.28 

Constantin Bele, Adela Pintea, Francisc Dulf, Dan Lupu, Alexandru R. 

Biris 

2-P009 Monitoring of Ferulic Acid Content During the Malt Production ................2.29 

Sylvie Běláková, Renata Mikulíková, Zdeněk Svoboda, Simona Macuchová 

1


1 

2-P010 Monitoring of Sensorially Active Sulphur Compounds in Malt 

and Beer ................................................................................................................2.30 

Sylvie Běláková, Renata Mikulíková, Zdeněk Svoboda, , Simona Macuchová 

2-P011 Content of Strobilurin Fungicides in Barley Malt and Beer ..........................2.31 

Sylvie Běláková, Renata Mikulíková, Zdeněk Svoboda, Simona Macuchová 

2-P012 The Effect of Fermentation Conditions on the Yeast Aroma Profile ............2.32 

Emília Breierová, Kornélia Nemcová, Jaroslava Kaňuchová Pátková, Ingrid 

Vajciková 

2-P013 Biogenic Amines Reduction by Probiotic L. casei During Ripening 

of Fermented Sausages ........................................................................................2.33 

Radka Burdychová, Vlastimil Dohnal, Petra Hoferková, Pavla Sládková 

2-P014 Testing of Different Sacharomyces Species for the Ability to Sorb 

DeoxynivAlenol....................................................................................................2.34 

Radka Burdychová 

2-P015 Separation of Complex Oligosaccharides from Worth and Beer 

Using HPLC ..........................................................................................................2.35 

Jana Cabálková, Janette Bobáľová 

2-P016 Traceability of Honey and Olive Oil Origin using SPME–GC×GC– 

TOFMS ...................................................................................................................2.36 

Tomas Cajka, Katerina Riddellova, Jana Hajslova 

2-P018 Extention of Asparaginase Application to Acrylamide 

Minimization from Potato to Cereal Products ................................................2.37 

Zuzana Ciesarová, Kristína Kukurová, Alena Bednáriková 

2-P019 Amino Acids Profile of Selected Wholegrains Important to 

Acrylamide Formation in Cereal-based Products ..........................................2.38 

Zuzana Ciesarová, Kristína Kukurová, Alena Bednáriková, Peter Hozlár 

2-P019 Characterization of Beta-Carotene Enriched Biomass Production 

by Red Yeasts ........................................................................................................2.39 

Martina Čarnecká, Andrea Hároniková, Terezie Dvořáková, Andrea 

Halienová, Ivana Márová, Emília Breierová 

2-P020 Monitoring of Polycyclic Aromatic Hydrocarbons and Pesticides 

Occurring in Oils and Fish Products .................................................................2.40 

Lucie Drábová, Marie Suchanová,Vladimír Kocourek, Jana Hajšlová


2-P021 Biogenic Amines in Cheeses and Human Health ...........................................2.41 

Eva Dudriková, Zuzana Dičáková 

2-P022 Phytosterol and Fatty Acid Profile of Four Edible Functional Oils 

Processed in Romania .........................................................................................2.42 

Francisc Vasile Dulf, Carmen Socaciu, Constantin Bele, Adela Pintea, 

Mihaela L. Ungureşan 

2-P023 Antioxidant Activity of Flavanols From Grape Seed Extracts ......................2.43 

Rugina Dumitrita, Simona Vicas, Carmen Momeu, Carmen Socaciu 

2-P024 Influence of Long-Term Storage Onditions on Antioxidant and 

Other Active Component Content in Several Sorts of Apples .......................2.44 

Kateřina Duroňová, Kateřina Pařilová, Andrea Halienová, Jitka Dvořáková, 

Radka Kočí, Jan Goliáš, Ivana Márová 

2-P025 Content of Polyphenols and Antiradical Activity of bee Pollen ..................2.45 

Katarína Fatrcová-Šramková, Janka Nôžková, Magda Máriássyová, 

Miroslava Kačániová, Eva Dudriková 

2-P026 Reduction Power, Polyphenols Content and Antimicrobial 

Activity of Honey ...............................................................................................2.46 

Katarína Fatrcová-Šramková, Janka Nôžková, Miroslava Kačániová, Magda 

Máriássyová, Eva Dudriková 

2-P027 omparison of pectate hydrolases from parsley root cells ...............................2.47 

D. Flodrová, E. Stratilová, S. Garajová and J. Omelková 

2-P028 Mechanism of Bi Bi Reaction of Xyloglucan Endotransglycosylase 

(XET) from Petroselinum Crispum Roots .........................................................2.48 

S. Garajová, D. Flodrová, V. Farkaš and E. Stratilová 

2-P029 Multispeciation in Wines by Orthogonal Chromatograpy Coupled 

to UV, Refraction Index and ICP-MS Detectors ...............................................2.49 

T. García-Barrera, J. L. Gómez-Ariza 

2-P030 Identification of Cu-Metalloproteins in Mus Musculus Brain 

Extracts by Reversed-Phase HPLC Coupled with ICP-MS and ESI- 

Qq-MS ....................................................................................................................2.50 

T. García-Barrera, M. Gónzalez-Fernández, J. López-Barea, C. Pueyo and J. 

L. Gómez-Ariza 

1


1 

2-P031 Analysis of Flavor Compounds in Fruits by GC with two 

Dimensional Detection by FID and MS ............................................................2.51 

T. García-Barrera, R. Jara Biedma, J. L. Gómez-Ariza 

2-P032 Content of Cadmium in selected Meat Products Supplied on the 

Slovak Marked .....................................................................................................2.52 

Jozef Golian, Branislav Šiška, Robert Toman, Michal Bošiak 

2-P033 Chiral Speciation of Iodine in Thyroid Hormones by Using HPLC- 

MS/MS and HPLC-ICP-MS .................................................................................2.53 

J. L. Gómez-Ariza, E. Gómez de las Heras, T. García-Barrera 

2-P034 Development of a rapid arsenic Speciation Procedure for Marine 

Origin Food based on Ultrasound Probe Extraction and HPLC- 

UV-HG-AFS .........................................................................................................2.54 

J. L. Gómez-Ariza, M. Contreras-Acuña, T. García-Barrera 

2-P035 Comparison of Proteome and Metabolome Changes in Stressed 

Yeast Strains Rhodotorula Glutinis and Rhodotorula Rubra ........................2.55 

Andrea Halienová, Ivana Márová, Zbyněk Zdráhal, Hana Konečná, Martina 

Čarnecká, Kateřina Pařilová, Emília Breierová 

2-P036 (1→3)(1→4)-β-D-Glucan: Variability and Factors Affecting Its 

Content in Oat Grain ...........................................................................................2.56 

Michaela Havrlentová, Ján Kraic, Milan Čertík 

2-P037 Extraction and Determination of Flavonoids in Plant Materials ..................2.57 

Barbora Hohnová , Pavel Karásek, Milena Vespalcová 

2-P038 Monitoring of Pesticide Residues in Commodities Used for Baby 

Food Production ...................................................................................................2.58 

Jiří Honzíček, Jana Hajšlová, Vladimír Kocourek 

2-P039 Trichosporon Cutaneum: Cell Adhesion on Cellophane Surface .................2.59 

J. Hrdinová, T. Krulikovská,V. Jirků, O. Schreiberová, A. Čejková, J. Masák 

2-P040 Seasonal Changes of Rubisco Activity and its Content in Norway 

Spruce Exposed to Ambient and Elevated CO 2 Concentrations ...................2.60 

Miroslav Hrstka, Lucie Zachová, Otmar Urban 

2-P041 Enzyme Degradation of LIPIDS and its Application in lipid 

–Containing Wastewater ...................................................................................2.61 

Kateřina Illková, Jiřina Omelková, Bohumila Vlčková


2-P042 Gas Chromatography-Mass spectrometry Characterization of Sea 

Buckthorn ..............................................................................................................2.62 

Andreea Iordache, Radu Minea, Monica Culea, Camelia Lehene 

2-P043 Rapid Authentification of Natural Juices by GC-MS .....................................2.63 

Andreea Iordache, Monica Culea, Onuc Cozar 

2-P044 Comparative Extraction Methods of some Biologic Active 

Compounds in Herbs .........................................................................................2.64 


2-P045 Bioremediation of Botton Sediments using Bacillus Megaterium 

and Bacillus Cereus .............................................................................................2.65 

Katarina Jablonovska, Iveta Styriakova 

2-P046 Biosorbents Preparation for Heavy Metals Removing from Waters ............2.66 

Jana Jenčárová, Alena Luptáková 

2-P047 Genotype Variability of Fatty Acids in Cereal Grains ....................................2.67 

Dalibor Ješko, Milan Čertík 

2-P048 Preliminary Simultaneous Studies on the Antifungal Activity 

and Chemical Composition of the Hexane Extract of the Brazilian 

Chenopodium Ambrosioides L. ..........................................................................2.68 

Gulab Newandram Jham, Carolina Marangon Jardim, Onkar Dev Dhingra 

and Marcelo Moreira Freire 

2-P049 Evaluation of antioxidant and antimicrobial effects of natural 

honeys ....................................................................................................................2.69 

Miroslava Kačániová, Martina Fikselová, Soňa Felšöciová, Vladimíra 

Kňazovická, Katarína Fatrcová-Šrámková, Janka Nôžková 

2-P050 Combined Technique LC/MS in Analyses of Antioxidant 

Compounds ...........................................................................................................2.70 

Radka Kočí, Marie Trčková, Lukáš Müller, Ivana Márová 

2-P051 Influence of Chemical Treatment on Fusarium Toxins in Barley 

Harvested 2005-2007 ............................................................................................2.71 

Jana Kohoutková, Kateřina Lancová, Alexandra Krplová, Ondřej Lacina, 

Jana Hajšlová, Jana Marková, Jaroslava Ehrenbergerová 

2-P052 Monitoring of Acrylamide in Foods : “The Project on Dietary 

Exposure of the Czech Population to Selected Chemical Substances” ........2.72 

J. Kolářová, S. Saláková, I. Řehůřková, J. Ruprich 

1


20 

2-P053 Trans Fatty Acids in Diet of the Czech Population During year 

2007 .........................................................................................................................2.73 

J. Kolářová, J. Hradilová, I. Řehůřková, J. Ruprich, S. Saláková, D. 

Pečinková 

2-P054 The Monitoring of Dietary Exposure of Czech Population to 

Persistent Organic Pollutants ............................................................................2.74 

Jana Kolářová, Sylva Saláková, Irena Řehůřková, Jiří Ruprich 

2-P055 Occurrence of Fusarium Mycotoxins and their Conjugated Forms 

in Cereal-based Foods ........................................................................................2.75 

Marta Kostelanská, Jana Hajšlová, Milena Zachariášová, Alexandra 

Krplová, Jan Poustka, Jana Kohoutková 

2-P056 Lignans in Flaxseeds – LC-MS/MS Determination .........................................2.76 

Anna Krajcova, Vera Schulzova, Jana Hajslova, Zuzana Zakova, Marie 

Bjelkova 

2-P057 The Occurence of PhthalicAcid Esters in Selected Feedstuffs .......................2.77 

Lenka Krátká, Alžbeta Jarošová, Jana Malyszová, Vlasta Stancová, Jiří 

Harazim 

2-P058 New Approach in Analysis of Fusarium Mycotoxins and their 

“Masked” Forms: Immunoaffinity Column Clean-up ...................................2.78 

A. Krplova, A. Kostelanska, M. Zachariasova, J. Hajslova and J. Kohoutkova 

2-P059 The effect of cell adhesion on polutant biodegradation ................................2.79 

Tereza Krulikovská , Olga Schreiberová, Jitka HrdinováJan Masák, Alena 

Čejková, Vladimír Jirků 

2-P060 Categorization of Olive Oils ..............................................................................2.80 

Dáša Kružlicová, Ján Mocák, Ernst Lankmayr 

2-P061 The Proteomic Analysis of Barley Albumins And Globulins ......................2.81 

Markéta Laštovičková and Janette Bobáľová 

2-P062 Comparative Study of The Protein Composition Induced By 

Malting of Two Barley Varieties .........................................................................2.82 

Markéta Laštovičková, Martina Šopíková, and Janette Bobáľová 

2-P063 fatty acids like markers of processed cheese changes during 

storage ....................................................................................................................2.83 

Blanka Loupancová, Eva Vítová, Hana Štoudková, František Buňka


2-P064 Determination of Selected Antioxidant Enzymes in Barley and 

Malt ........................................................................................................................2.84 

S. Macuchová, I. Márová, R. Mikulíková 

2-P065 Fat content and its quality in rapeseed (Brassica napus l) during 

storage ....................................................................................................................2.85 

Ján Mareček, Martina Fikselová, Helena Frančáková, Zdenka Muchová 

2-P066 Analysis of Active Substances in Honey – a Contribution to 

Honey Authenticity .............................................................................................2.86 

Ivana Márová, Zuzana Jelénková, Kateřina Duroňová, Petra Ždánská, 

Veronika Ehrenbergerová 

2-P067 Antioxidant and Antimutagenic Activity of Dried Fruit, Fruit Teas 

and Cereal Fruit Products ..................................................................................2.87 

Ivana Márová, Michaela Barošová, Martina Tomková, Hedvika Vondráčková, 

Kateřina Duroňová, Radka Kočí 

2-P068 Charactzerization of „Czech Beer“ – a Pilot Study ........................................2.88 

Ivana Márová, Renata Mikulíková, Zbyněk Zdráhal, Hana Konečná, 

Kateřina Pařilová, Andrea Halienová 

2-P069 Tocopherol and Fatty Acid Contents of Selected Romanian Cereal 

Grains .....................................................................................................................2.89 

Cristian Tudor Matea, Ioan Has, Simona Ifrim, Constantin Bele 

2-P070 Monitoring of Barley Starch Amylolysis by Gravitational Field 

Flow Fractionation and MALDI-TOF/TOF MS ................................................2.90 

Karel Mazanec and Janette Bobáľová 

2-P071 Wood Biomass in Cosmetics Industry ..............................................................2.91 

Radoslav Mikulášik, Jana Kosinková, Aleš Ház, Jarmila Hojerová, Svetozár 

Katuščák, Igor Šurina 

2-P072 Termogravimetric Analysis of Liquid Pyrolysis Products of Wood 

Biomass .................................................................................................................2.92 

Radoslav Mikulášik, Aleš Ház, Peter Šimon, Svetozár Katuščák, Igor Šurina 

2-P073 Analytical Meassurements and Phisicochemical Parametrs 

Determination in Strawberries and Raspberries Enhance Production .......2.93 

Fernando R. Moreno, A. Gago, T. García-Barrera, J. L. Gómez-Ariza 

2-P074 Multivariate Methods in Food Analysis ...........................................................2.94 

Viera Mrázová, Ján Mocák, Katja Šnuderl, Ernst Lankmayr 

21


22 

2-P075 Phytochemical Study of Flavonoides from Melampyrum 

Cristatum L. Species ............................................................................................2.95 

M. F. Munteanu, L. Vlase, C. Toma 

2-P076 The Influence of Choice Factors on Fermentation of Red Wine ...................2.96 

J. Omelková, L. Zechmiestrová 

2-P077 Optimalization of Cultivation Media and Hydroxylase Enzyme 

Production by Aureobasidium Pullulans .........................................................2.97 

J. Omelková, S. Matalová, I. Šimkovic 

2-P078 Influence of Modified Biocomposites on Production of 

Extracellular Polysaccharides by Immobilized Aureobasidium pullulans ..2.98 

V. Ondruška, I. Márová, J. David, L. Vojtová 

2-P079 Fatty Acids Distribution in the Lipid Fractions of Calendula 

officinalis l. Seeds Oil ...........................................................................................2.99 

Adela Pintea, Francisc Dulf, Constantin Bele, Sanda Andrei 

2-P080 Lipid Peroxidation Products in Plasma of Patients with Chronic 

Pancreatitis ..........................................................................................................2.100 

M. Podborská, A. Lojek, L. Kubala, R. Buňková, I. Márová, A. Ševčíková, 

J. Trna, P. Dítě 

2-P081 The Effect of Lipophilicity on Antifungal Activity of Some 2- 

Amino and 2-Methylbenzimidazole derivatives ..........................................2.101 

Sanja O. Podunavac-Kuzmanović, Dragoljub D. Cvetković 

2-P082 QSAR Models for Predicting the Antibacterial Activity of Some 

1-Benzylbenzimidazole Derivatives ................................................................2.102 

Sanja O. Podunavac-Kuzmanović, Dragoljub D. Cvetković, D.Barna 

2-P083 Antimicrobial Investigations of Nickel(II) Complexes With Some 

1-Benzylbenzimidazole Derivatives ................................................................2.103 


2-P084 Cheese Species Identification by Multivariate Analysis of 

Elemental Data ...................................................................................................2.104 

Martin Polovka, Milan Suhaj, Mária Koreňovská


2-P085 Optimalization of Method for Quantification of Streptococcus 

Mutans to Dental Materials ..............................................................................2.105 

Ilona Prudíková, S. Matalová, Jiřina Omelková, Josef Jančář 

2-P086 BFRs Profiles in Breast Milk and Adipose Tissue Samples: Case 

Study Concerned with Czech Population ......................................................2.106 

Jana Pulkrabová, Petra Hrádková,Michaela Nápravníková, Jana Hajšlová, 

Jan Poustka 

2-P087 Iodine in the Czech Market Milk ....................................................................2.107 

Jana Řeháková, Radek Kavřík, Irena Řehůřková, Jiří Ruprich 

2-P088 Monitoring of Total Mercury Content in Foods Consumed by the 

Czech Population During 1994–2005 ...............................................................2.108 

J. Řeháková, I. Řehůřková, J. Ruprich, J. Kalvodová, D. Matulová 

2-P089 The Influence of Surface Characteristics on Bacterial Cell 

Adhesion .............................................................................................................2.109 

Olga Schreiberová, Tereza Krulikovská, Jitka Hrdinová, Jan Masák, Alena 

Čejková, Vladimír Jirků 

2-P090 HPLC Analysis of Flavonoids from Satureja Hortensis L. ..........................2.110 

Maria-Loredana Soran, Bros Ildiko, Constantin Bele, Emanoil Surducan, 

Vasile Surducan, Cristian Tudoran 

2-P091 Selection of Suitable Microorgamisms for Preparation of Lactic 

Acid Fermented Cabbage Juice ........................................................................2.111 

Ivana Šimonová, Jolana Karovičová, Mária Greifová, Zlatica Kohajdová 

2-P092 Using of Hydrocolloids for Delaing Staling of Bakery Products ................2.112 

Ivana Šimonová, Zlatica Kohajdová, Jolana Karovičová 

2-P093 Influence of Ph on the Rheology Properties of Tomato Ketchups ..............2.113 

Ivana Šimonová, Jarmila Lehkoživová, Jolana Karovičová, Jarmila Hojerová, 

Zlatica Kohajdová, 

2-P094 Distribution of Diazinon in Various Tissues and its Effect on 

Serum Cholinesterase after an Intraperitoneal Administration .................2.114 

Branislav Šiška, Jozef Golian, Robert Toman, Andrea Krajčírová 

2-P095 Identification of Bacterial Strains of Species Lactococcus Lactis in 

Hard Cheeses using PCR ..................................................................................2.115 

A. Španová , J. Herzogová, B. Rittich 

2


2 

2-P096 Identification of Bacterial Strains of Species Streptococcus 

thermophilus using PCR ...................................................................................2.116 

A. Španová, M. Tycová, B. Rittich 

2-P097 Study of Oxidation Stability of Selected Vegetable Oils ...............................2.117 

Hana Štoudková, Monika Maxová, Jiří Kučerík, Jana Zemanová 

2-P098 Antioxidant Properties of Grape Skins Extracts Investigated by 

EPR and UV-VIS Spectroscopy .........................................................................2.118 

Lenka Šťavíková , Martin Polovka 

2-P099 The Impact of Fermentaion Process on Amino Acids Profile in 

White Wine Veltlínské Zelené ..........................................................................2.119 

Lenka Šťavíková, Miroslav Hrstka 

2-P100 Characterization of Neutral Lipid Composition of Nigella Sativa 

L. Seeds Oil .........................................................................................................2.120 

Claudia-Crina Toma, Adela Pintea, Constantin Bele, Francisc Dulf . 

2-P101 Identification of Viable Lactobacillus Cell in Fermented Milk 

Products. ..............................................................................................................2.121 

S. Trachtová, A. Španová, B. Rittich 

2-P102 Dynamic of Selected Pesticides Applied to Apples Protection 

During Pre-Harvest and Post-Harvest Period ...............................................2.122 

Jana Urbanova, Ondrej Lacina, Jakub Schurek, Miroslav Lansky and Jana 

Hajslova 

2-P103 Relationship between Xanthohumol, Polyphenols and flavonoids 

content in hop Leaves and Cones with Regard to Cultivar and 

Vegetation Period ...............................................................................................2.123 

Eva Ürgeová, Peter Kulacs, Ľudovít Polívka 

2-P104 Amino Acid Composition of Non-Transgenic and Ovalbumine 

Expressing Transgenic Alfalfa (Medicago sativa). ........................................2.124 

Eva Ürgeová, Juraj Faragó, Miklós Fári, Natália Faragová 

2-P105 Sea Buckthorn – Source of Vitamin C and Efficient Antioxidant ................2.125 

Milena Vespalcová, Dana Vránová, Martin Polovka, Jiřina Endstrasserová, 

Vojtěch Řezníček


2-P106 Arsenic Speciation in Fish Samples Using High Performance 

Liquid Chromatography Coupled with Hydride Generation 

Atomic Fluorescence Spectrometry .................................................................2.126 

Eva Vitoulová, Blanka Macharáčková, Miroslav Fišera, Olga Čelechovská 

2-P107 Comparison of Aroma Profiles of Several Types of Dark Chocolate ..........2.127 

Eva Vítová, Blanka Loupancová, Hana Štoudková, Jana Zemanová, Libor 

Babák, Ivana Macků 

2-P108 Monitoring of Changes in Population of Yeasts During 

Fermentation of Grape Must. ...........................................................................2.128 

Dana Vránová,Michaela Zdeňková, Renata Vadkertiová 

2P109 Study of Stability of Cleansers Based on Active Chlorine............................2.129 

J. Zemanova, E. Vitova, M. Dankova, M. Popelkova 

2-P110 Vegetable Oils for Cosmetics – Evaluation of their Effects on 

Human Skin ........................................................................................................2.130 

J. Zemanova, H. Stoudkova, E. Vitova 

Section 3: Chemistry of InorganicMaterials 

Lectures 

3-L01 Non -Traditional Binders and Composites Tested at the Student 

Laboratories of the Iinstitute of Materials Chemistry, Faculty of 

Chemistry, Brno University of Technology ......................................................3.5 

Jiří Brandštetr, Jaromír Havlica, Tomáš Opravil, František Šoukal 

3-L02 Influence of Phosphate Content on Formation of Dicalciumsilicate 

Modifications .........................................................................................................3.6 

Jaromír Havlica, Teodor Staněk, Jan Baráček, Petr Ptáček, František Šoukal 

3-L03 The Efficacy of Deacidification by Aerosols of MgO and Dolomite 

Microparticles .........................................................................................................3.7 

S. Holubková, M. Jablonský, M. Bajziková, J. Hanus, J. Rychlý, V. Bukovský, 

S. Katuscak 

3-L04 Distribution of Heavy Metals in the Products of Magnetic 

Separation of Siderite Ore From Nižná Slaná ....................................................3.8 

Slavomír Hredzák, Michal Lovás, Štefan Jakabský, Dana Gešperová 

2


2 

3-L05 Magnetically Modified Bentonite and Study of its Improved 

Sorption Properties ................................................................................................3.9 

Z. Orolínová, A. Mockovčiaková 

3-L06 Reductive Dissolution of Ferric Sulfate and Ferric Iron 

Oxihydroxides by Acidophilic Heterotrophic Bacteria ..................................3.10 

Zuzana Pállová, Daniel Kupka, Alexandra Vašková, Zuzana Orolínová, 

Vladimír Šepelák. 

3-L07 The Effect of Hydrogen on Combustion of Natural Gas ...............................3.11 

Ondřej Prokeš, Daniel Tenkrát, Aleš Doucek 

3-L08 The IR Spectroscopy Study of Particle Size Influence on Course of 

Kaolin Dehydroxylation ......................................................................................3.12 

Petr Ptáček, Dana Kubátová, Jaromír Havlica, František Šoukal, Tomáš 

Opravil 

3-L09 Critical Particulates fineness of the Air Classification Processes .................3.13 

Tomáš Svěrák, Ondřej Kozdas, Vítězslav Frank 

3-L10 Determination the Reaction Order for Fast Formation of CuS 2 O 3 ................3.14 

Mihaela Ligia Ungureşan, Francisc Vasile Dulp 

3-L11 Ceramic Petrography of the Loštice Pottery ....................................................3.15 

Dalibor Všianský, Miroslava Gregerová, Martin Hložek, 

Posters 

3-P01 Optimalization of Lead Sulphide Mechanochemical Synthesis ...................3.19 

M. Achimovičová, A. Mockovčiaková, E. Dutková 

3-P02 Synthesis of Doped Lanthanum Ferrite Perovskites .......................................3.20 

Eva Bartoníčková, Jaroslav Cihlář 

3-P03 Effect of Heat Treatment on the Al + Si Diffusion Coatings on Ni- 

Based Alloys ..........................................................................................................3.21 

Ladislav Čelko, Lenka Klakurková 

3-P04 Chemical Analysis of Al and Si Surface Layers on Steels...............................3.22 

Pavel Doležal, Ladislav Čelko, Aneta Němcová, Lenka Klakurková 

3-P05 Synthesis of Belite Cement from Coal Fly Ash ...............................................3.23 

Ivana Fiľková, Nadežda Števulová


3-P06 Microbiologically influenced corrosion of concrete .......................................3.24 

Vlasta Harbuľáková, Adriana Eštoková, Nadežda Števulová, Alena 

Luptáková 

3-P07 Influence of Salts Admixtures on Gelation Process of Silica ........................3.25 

Jaromír Havlica, Petr Ptáček, František Šoukal, Martin Zmrzlý 

3-P08 Thaumasite preparation ......................................................................................3.26 

Markéta Hermanová, Vítězslav Frank 

3-P09 An Application of Al-Si Layer on Nickel-Based Superalloys and 

their Analysis ........................................................................................................3.27 

Martin Juliš, Simona Pospíšilová 

3-P10 Alkaline Modified Coal Fly Ash as an Addition to Concrete ........................3.28 

Jozef Junák, Nadežda Števulová 

3-P11 Elements Interaction Analyses During Preparation of Al-Al 3 Ni 

Eutectic Composites .............................................................................................3.29 

Lenka Klakurková, Ladislav Čelko, Pavel Doležal, Ondřej Man, Karel Němec 

3-P12 Magnetic Properties of Waste Vitrified in Microwave Furnace.....................3.30 

Milota Kováčová, Michal Lovás, Marek Matik, Vladimír Šepelák 

3-P13 The use of Electrokinetic Potential Measurement for Examination 

of Kaolinite Decomposition ................................................................................3.31 

Dana Kubátová, Petr Ptáček, Jaromír Havlica. 

3-P14 Analysis and Interpretation of Height Alumina Refractories 

Corrosion by Alkali and Vanadium Oxides .....................................................3.32 

Tomáš Opravil, Petr Ptáček, Jaromír Havlica,František Šoukal, Michal 

Vršecký 

3-P15 Measurment of Heat of Hydratation of Roman Cement ...............................3.33 

Tomáš Opravil, Petr Ptáček, František Šoukal, Jaromír Havlica 

3-P16 FT-IR Spectroscopic Study of Portland Cement Hydration ...........................3.34 

Tomáš Opravil, Petr Ptáček, Jaromír Havlica, František Šoukal 

3-P17 Synthesis of Calcium, Magnesium and Zinc Negative Thermal 

Expansion LAS Ceramics ....................................................................................3.35 

Petr Ptáček, Helena Krejčová, Jaromír Havlica, František Šoukal, Tomáš 

Opravil 

2


2 

3-P18 Catalytic Actitity of Titania-Kaoline Composite..............................................3.36 

Petr Ptáček, Michal Fiala, Jaromír Havlica ,František Šoukal, Tomáš Opravil 

3-P19 Deposition of the TiO 2 on a Glass and Ceramics Surface ...............................3.37 

Alena Rutteová, Jaromír Havlica, Petr Ptáček 

3-P20 Composition Changes of Clinker with P 2 O 5 Content .....................................3.38 

Theodor Staněk, Petr Sulovský, Lenka Tomancová 

3-P21 Spectroscopic Investigation of some Transitional Metals 

Complexes with Leucine as Ligand ..................................................................3.39 

A. Stanila , A. Marcu, D. Rusu, M. Rusu 

3-P22 The Study of Compressive and Flexural Strength of High 

Performance Concrete (HPC) as the Function of its Composition................3.40 

Pavel Šiler, Josef Krátký, Ondřej Veleba, Pavel Jedlička 

3-P23 Temperature and Moisture Effects on Macrodefct-Free Composite 

Structure and Properties ....................................................................................3.41 

František Šoukal, Jiří Másilko, Jaromír Havlica, Petr Ptáček, Tomáš Opravil 

3-P24 Latex Modified Cement Composites: Effect of Polymer Type .......................3.42 

František Šoukal, Václav Vinter, Jaromír Havlica, Petr Ptáček, Tomáš 

Opravil 

3-P25 Influence of Conditions on Hydraulic Calcium Aluminate Phases 

Hydration Mechanisms ......................................................................................3.43 

František Šoukal, Jan Koplík, Jaromír Havlica, Vítězslav Frank, Petr Ptáček 

3-P26 The Study of Building Materials Biodeterioration by Inovatives 

Methods ................................................................................................................3.44 

Eva Terpáková 

3-P27 Milling of Olivine (Mg, Fe) 2 SiO 4 in High-Energy Mills by Wet and 

Dry Way .................................................................................................................3.45 

Erika Turianicová, Peter Baláž 

3-P28 Numerical Modelling and Simulation for Interaction between Cu2+ and S O 2 3 

Mihaela Ligia Ungureşan, Mihail Abrudean, Paula Raica, Eva Dulf, Tiberiu 

Coloşi 

2- ...............................................................................................................3.46 

3-P29 Bioactivity and Cytotoxicity of Fluorhydroxyapatite Ceramic .....................3.47 

Martin Vitkovič, Maha Salih Mahammed Noaman and Martin Palou


3-P30 Microwave-Hydrothermal Synthesis of Hydroxyapatite From 

CaSO 4 · 1/2H 2 O ......................................................................................................3.48 

P. Zamazalová , Ch. Damia, E. Champion, O. Gedeon 

3-P31 Conversion Coatings on Magnesium Alloys....................................................3.49 

Martin Zmrzlý 

Section 4: Chemistry of OrganicMaterials 

Lectures 

4-L01 Effect of Water Dissolution and Freeze-Drying on the Solid State 

NMR Sensitivity of a Heterogeneous Organic Mixture ...................................4.5 

Pellegrino Conte, Anne E. Berns,Peter Burauel, Hans-Dieter Narres, Harry 

Veerecken 

4-L02 Aminoacid Profiles Monitoring for Diagnosis ..................................................4.6 

Monica Culea, Andreea Iordache, Cornelia Mesaros 

4-L03 Microwave-Assisted Extraction of Organic Compounds from the 

Brown Coal ..............................................................................................................4.7 

Silvia Čuvanová, Michal Lovás 

4-L04 Reactivity of N-NO 2 Bonds in Nitro Amides ...................................................4.8 

Zdeněk Friedl, Svatopluk Zeman 

4-L05 XRD and FT-IR Study of Soot Obtained from Pyrolysis of Used 

Tires ..........................................................................................................................4.9 

Slavomír Hredzák, Silvia Ivanová 

4-L06 Determination of Yield and Chemical Composition of Soda- 

Anthraquinone Semichemical Pulp from Hardwood Mixture ....................4.10 

Michal Letko, Erika Nováková, Milan Vrška 

Posters 

4-P01 Efficient Solvent-Free Synthesis of Bis(arylmetylidene)piperidinon 

es .............................................................................................................................4.13 

M. Saeed Abaee, Mohammad M. Mojtahedi, Roholah Sharifi, A. Wahid 

Mesbah 

4-P02 Novel Diketopyrrolopyrroles for Molecular Optical and Electrical 

Devices ...................................................................................................................4.14 

Pavel Bednář, Oldřich Zmeškal, Martin Weiter, Martin Vala 

2


0 

4-P03 Diagnosis of Cirrhosis by GC/MS .....................................................................4.15 

Monica Culea, Cornelia Mesaros, Eugen Culea 

4-P04 Leukotriene Quantitation by GC-MS ................................................................4.16 

Monica Culea, Eugen Culea, Paraschiva Chereches-Panta, Adela Pintea 

4-P05 Characterization of Oligosaccharides Using ESI-MS in Presence of 

Anions ....................................................................................................................4.17 

Richard Čmelík, Janette Bobáľová 

4-P06 A new Approach to Protein Enzymatic Digestion for Fast Protein 

Identification by Matrix-Assisted Laser Desorption/Ionization 

Time-of-Flight Mass Spectrometry ....................................................................4.18 

Filip Dyčka, Markéta Laštovičková, and Janette Bobáľová 

4-P07 The 13 C and 119 Sn NMR Spectra of Some 

Trioraganotin-(IV)-N,N-Diethyldithiocarbamates Used as Wood 

Preservatives .........................................................................................................4.19 

Juraj Kizlink, Ladislav Reinprecht 

4-P08 Preparation of Dimethyl Carbonate from Methanol and Carbon 

Dioxide – The Removal of Reaction Water by Olefins ....................................4.20 

Juraj Kizlink 

4-P09 Amadori Rearrangement for Synthesis of Chiral Fragments .......................4.21 

Jana Marková, Milan Potáček 

4-P10 Solvent-Free Oxidative Deprotection of Trimethylsilyl Ethers with 

Calcium Hypochlorite .........................................................................................4.22 

Mohammad M. Mojtahedi, M. Saeed Abaee, Hassan Abbasi 

4-P11 Ionic Liquid Promoted Synthesis of Benzofurans at Room- 

Temperature .........................................................................................................4.23 

Ali Sharifi, M. Saeed Abaee, Bahram Zamiri, Mojtaba Mirzaei 

4-P12 Crystal Engineering of Glycoluril Dimers ........................................................4.24 

Marek Štancl, Vladimír Šindelář


Section 5: Photochemistry 

Lectures 

5-L01 EPR and UV Study on Photocatalytic Activity of Titanium 

Dioxide Powders ...................................................................................................5.5 

Vlasta Brezová, Zuzana Vrecková, Dana Dvoranová 

5-L02 Photocatalytic Degradation of Formic Acid on TiO 2 thin Layers ...................5.6 

Jana Chomoucká, Petr Dzik, Michal Veselý, Eric Puzenat and Chantal 

Guillard 

5-L03 Kinetic of Degradation of Historical Documents Containing Iron- 

Gall Inks ...................................................................................................................5.7 

Michal Čeppan, Viera Jančovičová, Milena Reháková, Andrej Buzinkay 

5-L04 Photochemical Transformations of Anticancer Drug Irinotecan .....................5.8 

Dana Dvoranová, Vlasta Brezová, Zuzana Vrecková, Marián Valko 

5-L05 Thin Layers of TiO 2 Prepared by Inkjet Printing ...............................................5.9 

Petr Dzik, Jana Chomoucká, Michal Veselý 

5-L06 Photocatalytic Properties of TiO 2 and Photosensitizing Materials 

for Bactericidal Applications ..............................................................................5.10 

C. Guillard, K. Benabbou, T.-H. Bui, T. Pigot, S. Pigeot-Remy, P. Lejeune, 

S. Lacombe 

5-L07 Promising Perspectives in Photocatalysis ........................................................5.11 

Chantal Guillard, Eric Puzenat, Jean-Marie Herrmann 

5-L08 Interactions in Iron Gall Inks in Air and Nitrogen Atmosphere ...................5.12 

Viera Jančovičová, Michaela Ciglanská, Bohuslava Havlínová, Michal 

Čeppan 

5-L09 Reactivity of Hydroxyl Radical on Benzene Ring and Side Chain 

in the Photocatalytic Degradation of Phenylurea Derivatives ......................5.13 

Jaromír Jirkovský, Gottfried Grabner 

5-L10 Approaches to standard photocatalyst tests in gaseous and liquid 

phase .....................................................................................................................5.14 

Josef Krýsa 

5L-11 Testing network for verification of photo-catalytic products and 

technologies for environmental benefit – PETV ..............................................5.15 

František Peterka 

1


2 

5-L12 Evaluation of the Photocatalytic Properties of TiO 2 Thin Film 

Layers. Application to Self-cleaning Glass .......................................................5.16 

E. Puzenat, J. Drbohlavová, L. Péruchon, J.-M. Herrmann and C. Guillard 

5-L13 Study of Stabilization of Documents Containing Iron Gall Inks by 

Treatment of Atmospheric DBD N 2 Plasma .....................................................5.17 

Milena Reháková, Michal Čeppan, Milan Mikula 

5-L14 An Overview of Inkjet Printouts Accelerated Ageing Methods ...................5.18 

Jiří Stančík, Michal Veselý, Petr Dzik 

5-L15 Subjective and Objective Evaluation of Recognisability of Black 

Character on Paper ...............................................................................................5.19 

A. Szitás, M. Jablonský, S. Katuščák S., 

5-L16 Pigments for Photocatalytic Paints ...................................................................5.20 

Jan Šubrt 

5-L17 Organometallic Cationic Photoinitiators ..........................................................5.21 

Jan Vališ, Bohumil Jašúrek, Tomáš Syrový, Katarína Remenárová, Tomáš 

Weidlich 

5-L18 Kinetics of Oxidative Processes on Inkjet-Printed thin Layers of 

Titanium Dioxide ................................................................................................5.22 

Michal Veselý, Petr Dzik, Mária Veselá, Jana Drbohlavová, Jana Chomoucká 

5-L19 Model dyes in Titania photocatalytic systems .................................................5.23 

Jiří Zita and Josef Krýsa 

5-L20 A Study on the Thickness Homogeneity and Refractive Index of 

Thin Organic Layers ...........................................................................................5.24 

Oldřich Zmeškal, Ota Salyk, Michal Veselý, Petr Dzik 

Posters 

5-P01 Using of Vis-Nir Fibre Optics Reflectance Spectra for 

Identification of Iron-Gall Inks in Historical Documents ...............................5.27 

Michal Čeppan, Daniela Pavlisová, Milena Reháková, Jarmila Dóriová 

5-P02 The Study of TiO 2 thin Films Photocatalytic Activity on 

Degradation of Yeast and Dye Pollutants .........................................................5.28 

Jana Chomoucká, Jana Drbohlavová, Petr Dzik, Mária Veselá, Michal Veselý


5-P03 The Influence of Photoinitiators and Aditives on the 

Photochemical Speed of Methacrylated PVAl .................................................5.29 

Marcela Černá, Petr Dzik, Michal Veselý 

5-P04 TiO 2 Based Sensor with Nanostructured Surface for Gas Detection ............5.30 

Jana Drbohlavová, Jaromír Hubálek, Marina Vorozhtsova, Radim Hrdý 

5-P05 Image Analysis of Offset Lithography Prints...................................................5.31 

Bohuslava Havlínová, Vladimír Dvonka, Ján Panák, Michal Čeppan 

5-P06 Color Fidelity of Reproductionof Historical Documents ...............................5.32 

Bohuslava Havlínová, Vladimír Dvonka, Michal Čeppan, Milena Reháková, 

Ján Panák 

5-P07 Improvement of Barrier Properties of Paper Using 

Photochemically Modified Polymer Layers .....................................................5.33 

Viera Jančovičová, Sabine Amberg-Schwab 

5-P08 Influence of Ageing on Optical Properties of Arylmethane Dyes .................5.34 

Viera Jančovičová, Miroslava Petrovičová, Bohuslava Havlínová, 

5-P09 Investigation of Decomposition of Photoinitiators .........................................5.35 

Bohumil Jašúrek, Tomáš Syrový, Jan Vališ, Tomáš Weidlich 

5-P10 Comparison of the Photocatalytic Properties of TiO 2 and Au/TiO 2 

in the Degradation of C 2 H 2 .................................................................................5.36 

E. Puzenat, C. Youssef, N. Jaffrezic-Renault, J.-M. Herrmann, C. Guillard a 

5-P11 Study of Influence of Iron-gall Inks Composition on Properties of 

Historical Documents ..........................................................................................5.37 

Milena Reháková, Michal Čeppan, Vladimír Dvonka 

5-P12 The Influence of Receiving Layer Composition on Inkjet Print 

Lightfastness ........................................................................................................5.38 


5-P13 Photopolymerization of Monomers with two Different Reactive 

Groups ...................................................................................................................5.39 

Josef Svoboda, Josef Knobloch 

5-P14 A new Aproach to Inkjet Prints Lightfastness Evaluation .............................5.40 

Michal Veselý, Petr Dzik, Jiří Stančík


Section 6: Physical and Applied Chemistry 

Lectures 

6-L01 Self-Aggregation of Humic and Fulvic Acids Studied on IHSS 

Standards .................................................................................................................6.5 

Martin Drastík, Anna Čtvrtníčková, Jiří Kučerík 

6-L02 Robust Nonlinear Control of a Separation Column for 13 C 

Enrichment by Cryogenic Distillation of Carbon Monoxide ...........................6.6 

Eva-Henrietta Dulf, Clement Festila, Francisc Dulf 

6-L03 Molecular Characterisation of Lignite Humic Acids by Chemical 

Degradations ...........................................................................................................6.7 

Laurent Grasset, Dana Válková, André Amblès 

6-L04 Prediction of the Acetic and Formic Acid Formation in the Paper 

During the Accelerated Ageing by the Change of Optical Properties ...........6.8 

M. Jablonský, K. Hroboňová, J. Lehotay, S. Katuščák 

6-L05 Effect of Modification of Paper with Antioxidant of Piperidine 

Serie on Recognisability of Black Characters on Paper ....................................6.9 

Jana Kazíková, Attila Szitás, Katarína Vizárová, Michal Jablonský, František 

Považanec, Soňa Kirschnerová, Svetozár Katuščák 

6-L06 Role of Aromaticity in Humic Substances Degradation Kinetics 

UsingNon-Arrhenius Temperature Functions .................................................6.10 

Jiří Kislinger, František Novák, Jiří Kučerík 

6-L07 NMR Spectroscopy of Minor Compounds in Wine – a 

Comparison of Different Pre-Concentration methods ..................................6.11 

M. Mazur, K. Furdíková, M. Kaliňák, M. Valko, V. Žúbor, N. Prónayová 

6-L08 Hydrophobization of Paper by Vapours in N 2 Plasma at 

Atmospheric Pressure .........................................................................................6.12 

Milan Mikula, Alexandra Valaseková,Zuzana Jakubíková, Viera Jančovičová 

6-L09 Fluorescence Study of Polysaccharide in Dilute Aqueous Solution .............6.13 

Filip Mravec, Tereza Halasová, Miloslav Pekař, Vladimír Velebný 

6-L10 Spin-Trapping Investigation of Radical Intermediates Generated 

from Phenols.Specific Behaviour of Para-Methyl Phenols .............................6.14 

Ladislav Omelka, Petr Majzlík,Renata Superatová, Petra Holubcová 

6-L11 Vortex-Fractal-Ring Structure of Molecule ......................................................6.15 

Pavel Ošmera


6-L012 Hydration of Hyaluronan ...................................................................................6.16 

Alena Průšová, Petra Bursáková, Jiří Kučerík 

6-L13 Electrochemical Analysis of New Indole-Based Antioxidants .....................6.17 

P. Rapta, M. Zalibera, V. Šnirc, S. Štolc 

6-L14 New Colloidal Systems made of Humic Acids ...............................................6.18 

Petr Sedláček, Martina Klučáková, Miroslava Malenovská 

6-L15 Photochemical and Free Radicals Study of Cyanobacteria using 

EPR Spectroscopy .................................................................................................6.19 

Stopka P., Maršálek B., Křížová J. 

6-L16 Molecular Electronic: Advances and Limitations, Strategies, 

Materials, Methods and Applications ...............................................................6.20 

Martin Vala, Martin Weiter, Oldřich Zmeškal, Ota Salyk 

6-L17 Mutual Relation between Fractal and Statistical (Random, 

Thermodynamic) Phenomena in Nature ..........................................................6.21 

Oldřich Zmeškal, Martin Weiter,Martin Vala, Tomáš Bžatek 

Posters 

6-P01 Influence of Regenerated Humic Acids on Polyvinyl Alcohol 

Thermo-Oxidational Stability .............................................................................6.25 

Barbora Bakajová, Zoja Vlčková, Jiří Kučerík 

6-P02 Determination of Particle Shape and Size Distribution ................................6.26 

Edita Bretšnajdrová, Ladislav Svoboda, Jiří Zelenka 

6-P03 Hydration of Regenerated Humic Substances Considering their 

Biological Properties ............................................................................................6.27 

Petra Bursáková, Zoja Vlčková, Zdeněk Cihlář, Jiří Kučerík 

6-P04 Evaluation of Sorption Abilities of Natural Lignite for Organic 

Substances .............................................................................................................6.28 

Petra Bušinová, Miloslav Pekař 

6-P05 Maximum Bubble Pressure and the du noüy Platinum Ring 

Method of Surface Tension Measurements of Sodium Dodecyl 

Sulfate and Sodium Hyaluronate ......................................................................6.29 

Martin Chytil, Jitka Krouská, P. Kulilová, Miloslav Pekař 

6-P06 Surface Tension of Regenerated Humic Acids Salts .......................................6.30 

Anna Čtvrtníčková, Jiří Kučerík


6-P07 Immobilization of Lignite into PVAl Sponges .................................................6.31 

Petr Dzik, Václav Mach, Michal Veselý 

6-P08 FTIR and SFS Spectra of Humic Acids Isolated from Lignite and 

Chernozem ............................................................................................................6.32 

Naděžda Fasurová and Ľubica Pospíšilová 

6-P09 Enhancement of Northern Softwood Bleached Kraft Pulp 

Accessibility and Acid Hydrolysis by Means of Ultrasonic Treatment .......6.33 

Michal Jablonský, Štefan Šutý, B. Švehla 

6-P10 Modelling of Complexation of Heavy Metals And Humic Acids 

with Utilization of High Resolution Ultrasound Spectroscopy ....................6.34 

Martina Klučáková, Jiří KučeríkMiloslav Pekař 

6-P11 Kinetics of Sorption of Metal Ions on Lignitic Humic Acids .........................6.35 

Martina Klučáková 

6-P12 Acid-base Properties of Fractionated Humic Acids ........................................6.36 

Martina Klučáková, Ondřej Pilný 

6-P13 HF and DFT Calculations of the Infrared and Raman Spectra of 

Lanthanide Complexes Cp* 2 LnCl 2 Li(OEt 2 ) 2 , where Ln = Sm, Nd .................6.37 

Ondřej Kroutil, Soňa Hermanová, Lukáš Richtera, Josef Jančář 

6-P14 Comparison of Various Plasmachemical Processes with Respect to 

the Gas Temperature ............................................................................................6.38 

L. Lazar, P. Synek, M. Karásková, M. Eliáš, O. Jašek, L. Kelar, V. Buršíková, 

P. Konupčík, L. Zajíčková 

6-P15 EPR Study of Nitroxide Radicals Generated from Some Secondary 

Alkyl-Aryl and Aryl-Aryl Amines ....................................................................6.39 

Petr Majzlík, Ladislav Omelka, Jan Světlík, Peter Rapta 

6-P16 Roughness of Coated Papers Treated in Atmospheric Plasma ......................6.40 

Milan Mikula, Zuzana Pobudová, Adam Gilan 

6-P17 Influence of Dye Structure on Its Decomposition by Electric 

Discharge in Water Solution ...............................................................................6.41 

Jana Pajurková, Zdenka Stará, František Krčma 

6-P18 Influence of Mmmk Neutralization on Stability of Models of 

Historical Documents ..........................................................................................6.42 

M. Petrovičová, B. Havlínová, H. Paulusová


6-P19 Influence of CSC Booksaver Deacidification Process on Stability of 

Arylmetane Dyes on Paper .................................................................................6.43 

M. Petrovičová, M. Ďurovič, E. Belányiová, B. Havlínová 

6-P20 Effect of Accelerated Ageing on Properties of Parchment .............................6.44 

M. Petrovičová, B. Marušicová, M. Ohlidalová, B. Havlínová, 

6-P21 Quality of Soil Humic Substances by SFS and UV-VIS 

Spectroscopy .........................................................................................................6.45 

Ľubica Pospíšilová, Naděžda Fasurová 

6-P22 Sensoric Properties of Aromatic and Heterocyclic Compounds 

with Conjugated Bonds .......................................................................................6.46 

Ota Salyk, Pavel Bednář, Oldřich Zmeškal, Jan Vyňuchal 

6-P23 Structure of 1,4-diketo-3,6-bis-(4´-dipyridyl)-pyrrolo-[3,4c]pyrrole 

and Relative Compounds ..................................................................6.47 

Ota Salyk, Vítězslav Frank, Jakub Stejkora, Jan Vyňuchal 

6-P24 The Influence of Mechanical Activation on the Production of the 

Humic Acids in the Brown Coal ........................................................................6.48 

M. Skybova, M. Lovas 

6-P25 Numeric Modelling of V-T Process in Nitrogen Ground State 

under Post-Discharge Conditions ......................................................................6.49 

Ivo Soural, František Krčma, Vasco Guerra 

6-P26 Evaluation of Intake Cappability of LCM Surface by Adsorption 

Dyeding Method and its Utilization for Evaluation of Changes in 

Surface Polarity Caused by DCSBD Low-Energy Plasma. ............................6.50 

Radovan Tiňo, Lívia Beňová,Svetozár Katuščák 

6-P27 Evaluation of Effiency, Release and Oxidation Stability of 

Seabuckthorn Microencapsulated Oil using Fourier Transformed 

Infrared Spectroscopy ..........................................................................................6.51 

Monica Trif and Carmen Socaciu 

6-P28 13C NMR Spectroscopy of Humic Acids Ectracted from Incubated 

Plant Residues in Haplic Luvisol ......................................................................6.52 

Anton Zaujec, Jozef Šima, Juraj Chlpík, Nora Szombathová, Erika Tobiašová


Section 7: Polymers and Polymer Composites 

Lectures 

7-L01 The Way of Stereoregularity Determination of Polypropylene ......................7.5 

Adam Hoza, Jan Kratochvíla, Soňa Hermanová 

7-L02 The Crystallization Kinetics in Semicrystalline Nanocomposites .................7.6 

Kateřina Hynštová, Jan Kalfus, Josef Jančář 

7-L03 Effect of Hexamethyldisiloxane Solution of Zirconium Propoxide 

on Permanence of Paper ........................................................................................7.7 

Soňa Kirschnerová, Katarína Vizárová, Svetozár Katuščák 

7-L04 Modification of Polypropylene Through Radical Grafting in Melt ................7.8 

František Kučera 

7-L05 Properties of Polypropylene Sheets Hot Compacted With 

Oriented Polypropylene Tapes .............................................................................7.9 

Jaroslav Kučera, Jiří Sadílek, Blanka Rýznarová 

7-06 Biodegradation of Modified Polyurethane Foams ..............................................7.10 

Obruča Stanislav, Márová Ivana, Ondruška Vladimír, Vojtová Lucy, David 

Jan 

7-L07 Reinforcing Mechanism in Layered Silicate Nanocomposites .....................7.11 

Lukáš Recman, Josef Jančář, Jan Kalfus 

7-L08 The Influence of Mg Compound Paper Modification on 

Hornification and Strength Properties During Accelerated Ageing ............7.12 

Štefan Šutý, Katarína Petriláková, Svetozár Katuščák, Soňa Kirschnerová, 

Michal Jablonský, Katarína Vizárová 

7-L09 Impact of Processing on Degradation of Polypropylene Impact- 

Copolymer .............................................................................................................7.13 

Jiří Tocháček, Soňa Hermanová, Josef Jančář, Jan Kalfus 

7-L10 Polymer Semiconductors for Future Molecular Electronic Devices .............7.14 

Martin Weiter, Martin Vala, Stanislav Nešpůrek 

7-L11 Molecular Modeling of Materials with Network Structure ...........................7.15 

Jan Žídek, Josef Jančář


Posters 

7-P01 Physico-Chemical Properties of Functionalized Temperature- 

Sensitive Biocompatible Block Copolymers .....................................................7.19 

Jan David, Lucy Vojtová, Jiří Kučerík, Ludmila Mravcová, Martin Chytil, 

Miloslav Pekař, Milada Vávrová, Josef Jančář 

7-P02 Properties of Recycled Carbon Fibers ...............................................................7.20 

Jan Grégr, Vladimír Kovačič, Miroslav Valeš, Bedřich Štekner, Karel 

Cihelník 

7-P03 Halosilyl-substituted Cyclopentadienyl Titanium Complexes as 

Catalysts for Styrene Syndiospecific Polymerisation ......................................7.21 

Soňa Hermanová, Jan Merna, Milan Erben, Zdeněk Svačina 

7-P04 Synthesis of Modified Cucurbit [n]Urils ..........................................................7.22 

Muhammad Shamsul Azim Khan, Vladimír Šindelář 

7-P05 Application of Thermochromic Compounds in Polymeric Materials .............7.23 

František Kučera, Petra Slánská 

7-P06 Polymer Photoelectronic Devices Based on Interaction Between 

π-Conjugated Polymer Matrices and Photochromic Molecules ...................7.24 

Jiří Navrátil, Martin Weiter, Martin Vala, Oldřich Zmeškal, Petr Toman, 

Stanislav Nešpůrek 

7-P07 Analysis of the Wood Surface Treated by Diffuse Coplanar 

Surface Barrier Discharge type Atmospheric Plasma .....................................7.25 

Erika Nováková, Michal Letko, Radovan Tiňo, Ján Marták, Lívia Beňová 

7-P08 Strategies for Enhancing Poly(3-hydroxybutyrate) Production in 

Selected Bacterial Strains .....................................................................................7.26 

Stanislav Obruča, Soňa Melušová, Ivana Márová, Zdeněk Svoboda 

7-P09 Comparison of Biodegradability of Modified Polyuretne Foams 

and Polyurethane Elastomeric Films .................................................................7.27 

Stanislav Obruča, Ivana Márová, Jana Piechová, Lucy Vojtová, Marek 

Novotný, Jan David 

7-P10 Synthesis of Substituted Polysilylenes Used as Semiconductive 

Polymers ...............................................................................................................7.28 

Imad Ouzzane, Soňa Hermanová, Martin Weiter, Stanislav Nešpůrek 

7-P11 Automation of PE-CVD Process for Preparing of Nanostructured 

Films .......................................................................................................................7.29 

Radek Přikryl


0 

7-P12 Crystal Structure of an Lanthanide Sandwich Complex 

(C5Me5)2NdCl2Li(OEt2)2...................................................................................7.30 

Lukáš Richtera, Soňa Hermanová, Vojtěch Jančík, Jaroslav Cihlář 

7-P13 Computing Effective Properties of Composites Via FEM 

Simulations ............................................................................................................7.31 

Miroslav Rolník, Jan Žídek, Josef Jančář 

7-P14 Effect of Thermal History on Structure-Property Relationship in 

Oriented Polypropylene Films and Tapes ........................................................7.32 

Jiří Sadílek, Jaroslav Kučera 

7-P15 Bioresorbable Collagen-Hyaluronic Acid Scaffolds Cross-Linked 

Using a Water-Soluble Carbodiimide ...............................................................7.33 

Alexandra Sloviková, Lucy Vojtová, Josef Jančář 

7-P16 Water Retention Value During Thermally Accelerated Ageing of 

Paper. ......................................................................................................................7.34 

Štefan Šutý, Katarína Petriláková, Svetozár Katuščák, Michal Jablonský, 

Katarína Vizárová., Soňa Kirschnerová 

7-P17 Spin Probe Dynamics in Relation to Free Volume and Relaxation 

Dynamics of Poly(isobutylene) ..........................................................................7.35 

Helena Švajdlenková, Josef Bartoš 

7-P18 Toughness Versus Strength and Stiffness of Polypropylene Filled 

with Submicron CaCO 3 Particles .......................................................................7.36 

Petr Veselý, Eva Nezbedová, Bohumil Vlach 

7-P19 Polysaccharides Modified Elastomeric Polyurethane .....................................7.37 

Lucy Vojtová, Marek Novotný, Stanislav Obruča, Jana Piechová, Ivana 

Márová, Josef Jančář 

7-P20 Models of Organized Supermolecular Structures: Application to 

Collagen Fibrils and Dendrimers .......................................................................7.38 

Jan Žídek, Jolana Ščudlová, Josef Jančář 

Appendices 

Index .................................................................................................................................. I.3 

Commercial presentations ............................................................................................... I.15

Preface 

1

Conference Partners 

2 THETA 

Analytical standards and equipment

Foreword 

Dear participants of the Chemistry and Life 2008 conference, 

Like three years ago, this year a special issue of the Chemické listy Journal 

with contributions presented at this 4 th successive conference Chemistry and Life 

has been delivered to you again. Unlike the previous conference contributions, this 

year’s ones have been published electronically on a CD, which might be appreciated 

by many of you, dear participants. 

Since 1999 the conference Chemistry and Life has regularly been organized by 

the Faculty of Chemistry of Brno University of Technology (BUT). It has become 

one of the most significant items in the field of science and research activities implemented 

by the faculty. Specialists have shown increasing interest in the conference 

and the present Organizing Committee has received almost 300 contributions that 

will be presented in 7 sessions. Besides the traditional attendance of Czech and 

Slovak chemists we also appreciate increasing number of contributing scientists and 

researchers from abroad. 

Fruitful cooperation with industry enables implementation of the conference 

in broad extent designed by the organizers. The conference committees are pleased 

to offer accompanying social programme that might contribute to successful course 

of the conference. 

Dear participants of the 4 th Chemistry and Life conference, on behalf of both 

the Organizing and Scientific Committees I feel honoured to welcome you to the 

grounds of the Faculty of Chemistry of BUT. At the same time let me express the 

hope that the conference outcomes will contribute to development of knowledge in 

various branches of chemistry. 

Assoc. Prof., Jaromír Havlica, Ph.D. 

Dean of the Faculty of Chemistry of BUT Brno 

Chairman of the Scientific Committee 

of the Chemistry and Life 2008 conference


P.1

P.2


PL-01 Functional Dairy Products – from Traditional to 

Modern Technology 

Irena Rogelj and Bojana Bogovič Matijašić 

University of Ljubljana, Biotechnical faculty, 

Department of Animal Science, Chair of Dairy Science, 

Groblje 3, 1230 Domzale, Slovenia 

Irena.rogelj@bfro.uni-lj.si 

Functional Food is a new chapter in nutrition science. It is usually described as 

“food which promotes health beyond providing basic nutrition”. From this point 

of view one could argue whether milk and many traditional dairy products belong 

to the family of functional food. However, it is more and more evident that almost 

all components of milk possess physiological functionality beyond nutrition. Moreover, 

several of them exert more than one physiological function. To date, different 

in vivo experiments including human clinical trials have generally supported the 

concept that dairy foods possess a natural “cluster” of nutrients that protect health 

and enhance the immune system. Bioactive peptides derived from milk proteins are 

a typical example. In addition, the milk and dairy products represent an excellent 

medium for the delivery of pro- and pre-biotics. Designing and developing functionality 

in dairy products simply means modifying and/or enriching the healthy 

nature of the original base. Progresses in food production and processing and modern 

biotechnology have been offering numerous opportunities for creation of “tailor 

made” functional dairy products. New in vitro and in vivo research methods and 

advanced models provide important information on the mechanisms behind the observed 

effects and enable the discovery of new biomarkers to be followed in human 

studies. In addition, different -omics are giving step by step more opportunities 

for scientific demonstration of health claims. Dairy products representing the major 

part of functional foods, especially fortified dairy foods, designer milk, fermented, 

probiotic and symbiotic dairy products will be presented and discussed. 

Key words: functional food, dairy products, biotechnology, probiotics, 

bioactive peptides 

P.


PL-02 Alkali-activated Aluminosilicates: Past, Present 

and Future 

P. 

Pavel Krivenko 

V. D. Glukhovsky State Scientific Research Institute for Binders and Materials, 

Kiev National University of Civil Engineering and Architecture 

Vozdukhoflotsky prospect, 31, Kiev, 03037, Ukraine 

pavlo.kryvenko@gmail.com 

Less than half a century ago an idea of the presence of free alkalis in the cement 

composition was considered by cement people as absurd one and this was a postulate 

of fundamental bases of revealing hydraulic binding properties by the mineral 

systems. At the same time, the studies held on the reasons for excellent durability of 

ancient cements together with data collected on stability and composition of natural 

mineral formations testified that this postulate was not correct. In 1957 the scientist 

from the USSR V.D.Glukhovsky put forward an idea about these contradictions and 

an assumption he made was taken as a base for studying, development and use of a 

principally new class of cements known in the art under the name-alkaline cements 

or in some publications- alkali- activated cements, in which the alkaline ions played 

role of not catalysts of reactions but as structure- forming elements. Further development 

of these binding systems and the experience obtained from their small- and 

large- scale application gave proofs to this assumption. 

Keywords: aluminosilicate, alkaline activator, alkaline cement, concrete, 

hydration


PL-03 Organic Anthropogenic Contaminants in 

River Systems – Complementary Environmental 

Approaches 

Jan Schwarzbauer 

Institute of Geology and Geochemistry of Petroleum and Coal, RWTH Aachen Unievrsity 

Lochnerstrasse 4-20, 52056 Aachen 

schwarzbauer@lek.rwth-aachen.de 

Rivers are sensitive but also complex ecosystems hosting a wide spectrum of 

organisms. Anthropogenic activities and the resulting discharge of contaminants 

have resulted in increasing river pollution over decades. Especially, in industrialized, 

densely populated or agricultural strongly affected regions the natural riverine 

organic matter is superimposed by huge anthropogenic emissions. 

Organic contaminants discharged to the aquatic environment exhibit a high diversity 

with respect to their molecular structures and the resulting physico-chemical 

properties. Although the chemical analysis of anthropogenic contamination in 

river systems is still an important feature, especially with respect to the identification 

and structure elucidation of novel contaminants, the focus of environmental 

studies has changed. In recent times the research on the environmental behaviour of 

organic pollutants became more and more important in order to assess their risk as 

a result of their emission to natural systems. Consequently, the knowledge not only 

about the occurrence and amount of anthropogenic contaminants but also about 

bioavailability, their exchange within different compartments, as well as on molecular 

aspects of degradation or metabolism processes increases significantly. Further 

on, environmental studies on the anthropogenic pollution of aquatic systems do not 

remain on tthe analysis of pre-selected substances. Considering the currently use of 

over 1000.000 man-made chemicals in the EU it has to be assumed, that analytical 

investigations on environmental systems solely focussed on a few pollutants still 

do not depict the ‘real’ pollution level. Therefore, the number of more extensive 

and detailed investigations considering a wide spectra of relevant organic pollutants 

increases slowly. Such investigations reveal a more comprehensive view on the 

state of pollution of a natural system. For all the described purposes, a complementary 

application of different analytical strategies and methods have to be applied 

to reveal a better understanding of environmental processes affecting the riverine 

pollution by organic substances. Examples of such approaches are presented in the 

following lecture. 

P.


PL-04 Dark Side of Chemistry in Human Civilization 

P. 

Pavel Častulík 

Brno University of Technology, Faculty of chemistry, 

Institute of Chemistry and Technology of Environmental Protection 

Purkynova 464/118, 612 00 Brno, Czech Republic 

castulik@fch.vutbr.cz 

As the Moon appears to human civilization as a planet with only one face towards 

to our eyes due to the Universe mechanics laws, some human societies would 

like to apply their subjective laws in order to keeping their superiority, by demonstrating 

only beneficial and peaceful face of chemistry. However, in the same time 

any advanced knowledge and technologies may have also display their dark side, if 

misused forcefully against others in order to keep dominancy above them. In nowadays 

the negative side of chemistry is affecting our living environment and lives due 

to negligence of subjects driven inter alia by commercial interests. Nevertheless, bitter 

experience in the use of chemistry in past war conflicts against humans giving us 

a serious warning on possible misuse of advanced nuclear, chemical and biological 

science and technology knowledge in unpredictable and covert consequences. 

On one side chemistry is helping to ease our life and speed up advanced development 

of human civilization and on the other side also manufacturing of weapons 

and their components and precursors. Traditionally the dark side of chemistry is 

linked within the chain of pharmacology and toxicology and their effects to living 

organisms. There is common ground between pharmacology benefits of drugs and 

toxicology effects due to the same chemical reactions/mechanism. Only differences 

between drugs and venoms are in their doses. 

In this presentation I wish to remind senior and junior scientific community 

on a very narrow razor-blade of balancing between benefits of chemistry and its 

potential evil role. We wish to go historically through events when chemical molecules 

had been used as mass killing chemical warfare and also on the other side 

of that coin when associated research in this field helps to increased agricultural 

production and advanced treatment of human diseases and more comprehensive 

understanding of metabolism and functions of human body. Memento will be given 

to potential threats of advanced chemistry science and technology, if misguided by 

humans against others and living environment.

PL-05 Challenges of the Analysis of Food and 

Environmental Matrices by GC-TOF MS 

and GCxGC-TOF MS 

Tomáš Kovalczuk, Jitka Zrostlíková, Jana Hajšlová, Jakub Schůrek, 

Mark Libardoni 

Leco Corporation, Apllication Laboratory Prague 

Sokolovská 219, Pratur 9-Vysočany, 1900 00 

Tomas.kovalczuk@leco.cz 


The demands on the analysis of organic contaminants’ residues have been 

increasing recently, taking into account the number of compounds analysed in a 

single run, number of matrices handled within one method, requirements on detection 

limits as well as requirements on the analysis speed. Accordingly, considerable 

progress in both sample preparation techniques and in the instrumentation has 

been done recently. 

Time-of-flight mass spectrometry offers several important benefits over conventional 

(scanning) MS detectors: (i) With TOF MS full mass spectral information 

is available across the whole chromatogram, while the achieved detectability is 

comparable to the one typical for conventional MS detectors. Therefore, unambiguous 

confirmation of analyte identity is possible according to full mass spectrum. 

(ii) The absence of spectral skewing and the constant ratios of ions across a chromatographic 

peak allow the application of software data mining algorithms of peak 

finding and true signal deconvolution. This enables the applications for non-target 

screening analysis. (iii) Being the fastest MS detector, time-of-flight can be used for 

the applications in fast and ultra-fast GC and comprehensive two-dimensional gas 

chromatography (GCxGC), as discussed below. 

Comprehensive two-dimensional gas chromatography (GCxGC) has been a 

subject of attention in recent years, because of its enormous separation ability. In 

GCxGC, two columns of different selectivity are connected via a thermal modulator, 

which cuts small parts of the first column eluate, focuses them to sharp zones and 

samples them onto the second column, where a very fast “flash” separation (100-150 

times faster compared to separation in first column) is performed. Each compound 

of a sample is therefore subjected to two independent separation mechanisms. 

The considerably increased peak capacity of GCxGC over one dimensional GC 

provides not only improved separation of target analytes but also target anaytes from matrix 

which allow more accurate identification and quantification of analytes. 

P.


PL-06 Nanoparticle Characterisation Using Light 

Scattering Techniques 

P. 

Michael Kaszuba 

Malvern Instruments Ltd., Grovewood Road, Enigma Business Park, Malvern, 

Worcestershire, WR14 1XZ. UK 

Particle size and zeta potential are important parameters for the characterization 

of nanoparticles. The technique of dynamic light scattering is well suited to the 

measurement of the size of nanoparticle dispersions. Traditionally, measurement of 

very small and/or poorly scattering particles or samples that are very dilute were difficult 

unless high-powered lasers were used. These problems have been addressed 

in a light scattering instrument incorporating novel non-invasive backscatter optics 

(NIBS®). This novel optic arrangement maximises the detection of scattered light 

while maintaining signal quality. 

Laser Doppler electrophoresis is an accepted method for the measurement 

of particle mobility and zeta potential of dispersions of colloidal size materials. A 

novel scheme using a combination of Phase Analysis Light Scattering (PALS) and 

a patented method of measurement called M3 to improve the accuracy and ease of 

use of the technique will be discussed. 

These technologies have been incorporated in the Zetasizer Nano Series of 

instruments from Malvern Instruments Ltd (Malvern, Worcestershire, UK). Examples 

of the application of these novel light scattering techniques to a wide range of 

nanoparticle samples will be presented which will illustrate the capabilities of the 

technology.

PL-07 Rapid stability assessment and particle 

characterization for food and nonfood 

products by multisample analytical 

centrifugation 

D. Lerche, T. Sobisch, S. Küchler, A. Uhl 

Prof. Dr. Dietmar Lerche, L.U.M. GmbH, Rudower Chaussee 29, 12489 Berlin 

www.lum-gmbh.com, info@lum-gmbh.de 


A considerable fraction of food products are dispersions (emulsions, suspensions, 

suspoemulsions). Therefore not only the microbiological and chemical stability 

matters, but the separation stability has also to be characterized. 

The development of new food formulations as well as process monitoring and 

quality assurance require efficient and objective analytical tools for shelf life prediction 

and stability determination. 

A new multisample approach is presented using analytical centrifugation, 

which allows for an accelerated characterization of destabilization processes (creaming, 

sedimentation, coalescence, phase separation). The kinetics of these destabilizing 

processes can be traced simultaneously for up-to 12 different samples with temperature 

control in the range from 4 °C to 60 °C. 

Analytical centrifugation measures the inherent stability of the samples, but 

cannot foresee destabilizing processes which will happen in the future. In this respect 

a combination with common accelerated stability testing is the method of 

choice. 

The wide application potential of this approach is demonstrated by examples 

of measurements on typical food dispersions like milk and milk based products. 

The characterization of cosmetic products or fine chemicals provide further insight 

into this analytical technique. 

P.


PL-08 Transformation of Petroleum Type Pollutant 

in the Environment 

P.10 

Branimir Jovancicevic 

Department of Chemistry, University of Belgrade, 

Studentski trg 12-16, Belgrade, Serbia 

bjovanci@chem.bg.ac.yu 

Petroleum and its refining products continue to be one of the most abundant 

environmental pollutants. Consequently, transformation processes of petroleumtype 

pollutants in soils, recent sediments, alluvial sediments, ground and surface 

waters were studied by numerous authors: in coastal marine environment, in estuarine 

sediments, under arctic marine conditions, on the East Mediterranean coast 

etc. 

The fate of an oil type pollutant in the environment can be monitored most accurately 

by determining its quantity and studying its composition in the polluted 

samples from the same or close locality, and during different periods of time. On 

that way a number of experiments were carried out in order to define the intesity 

and optimal conditions for the most efficient biodegradation (temperature, humidity, 

nutrients). 

On the other side, simulation of the natural conditions in the laboratory or by 

ex situ bioremediation with simultaneous intensification of only specified factors affecting 

the intensity and rate of transformation made it possible to save time and to 

come to relevant conclusions. Applications of biodegradation processes foccussed 

primarily on bioremediation of different sections of the environmental compartments 

have been described. 

In this paper our recent results will be reviewed observed by investigating tranformation 

processes of petroleum-type pollutant 1) by determining its quantity and 

studying its composition in the polluted samples from different distance of oil spill; 

2) by determining its quantity and studying its composition in the polluted samples 

during different periods of time from oil spill; 3) by simulation of the natural conditions 

in the laboratory and 4) by ex situ bioremediation with simultaneous intensification 

of only specified factors affecting the intensity and rate of transformation.

Section 1: 

Environmental Chemistry & 

Technology 

1.1

1.2

Lectures 

1.

Section 1 – Environmental Chemistry & Technology 

Lectures 

1-L01 Ecotoxicity vs. Contamination vs. Composition of 

River Sediments: the Quest for Determinants of 

Toxic Effects 

Luděk Bláha, Klára Hilscherová, Tomáš Čáp, Jana Klánová, 

Jiří Machát, Ivan Holoubek 

Masaryk University, RECETOX 

(Research Centre for Environmental Chemistry and Ecotoxicology) 

Kamenice 3, CZ62500 Brno, Czech Republic, 

blaha@recetox.muni.cz 

In this study, we present part of the results of a large-scale assessment of sediments 

in the Morava river basin (Zlín region, Czech Republic). Our aims were to 

evaluate novel rapid Flash assay, and to identify key sediment characteristics beyond 

the toxicity. Sediments from 14 localities (sampled 4-times at springs and autumns of 

2005 and 2006) were analysed for (i) geological and geochemical parameters (Corg, 

pH, silt, clay and sand size and % etc.), (ii) dominant contaminants (13 metals by 

ICP-MS, 16 US EPA PAHs, 14 PCBs and 9 OCPs by GC-MS and HPLC-UV/FD), 

and (iii) ecotoxicity (eluate toxicity with Vibrio fisheri, whole-sediment toxicity with 

the Flash assay, in vitro tests for dioxin toxicity, estrogenicity, mutagenicity etc.). 

Our critical evaluation of the Flash (based on a broad number of sediment samples) 

revealed good applicability of this whole-sediment test. However, we have found 

only minor correlations with the total levels of contaminants, and exchangable H+ 

was the only parametr (from more than 80 evaluated) that correlated with the toxicity. 

We conclude that ecotoxicity testing of the whole sediments is needed to obtain 

key data for complex risk evaluation, and rapid contact Flash assay may serve these 

purporses. Supported by the EU (FP6 project ECODIS, No. 518043-1), and by the 

Czech grants INCHEMBIOL (No. VZ0021622412) and ENVISCREEN (2B08036). 

1.


Lectures 

1-L02 Degradation Products of Synthetic Polymers as 

Emerging Environmental Contaminants 

1. 

Josef Čáslavský, Milada Vávrová, Daniela Mácová, 

Ludmila Mravcová 

Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, 


Purkyňova 118, 612 00 Brno, Czech Republic 

caslavsky@fch.vutbr.cz 

Synthetic polymers are widely used due to their excellent properties which 

can be “tailored” with respect to their planned use. The worldwide production of 

synthetic polymers is constantly increasing and these materials are used in a wide 

range of products. At the end of the product life cycle great part of the polymers is 

disposed on the waste dumps, where they remain usually for quite a long time and 

undergo various environmental degradation mechanisms. Due to huge amount of 

produced polymers it is advisable to evaluate the polymer degradation pathways, 

to identify the degradation products and to evaluate their possible environmental 

effects. 

In this study we focused our attention on the degradation mechanism and identification 

of degradation products of polyurethane foams, whose biodegradability 

was enhanced by the addition of biodegradable and bio-originated filler. 

Keywords: synthetic polymers, environmental degradation


Lectures 

1-L03 Usage of Gas Chromatography and IMS 

Detection for Evaluation of Polymer Barrier 

Material Properties 

J. Dvořáková, I. Mašek 

VOP-026 Šternberk, divison VTÚO, Veslařská 230, 637 00 Brno 

dvorakova.j@vtuo.cz 

Polymer barrier property against the highly toxic agents and industrial harmful 

substance is the phenomenon, which adjudicate about the quality of every protection 

means. By selection of the suitable polymer materials used for the protection 

means is needed to know not only their physical and chemical properties, but 

primarily their resistance against the permeation of toxic agents under short time 

and/or long time contamination in the gaseous and liquid phases. For comparison of 

the barrier materials from resistance against harmful substances the suitable methods 

for their evaluation is needed. The developed procedure enables to measure 

permeation of toxic agents through a given barrier materials. The results of permeation 

measurement of 2,2´-dichlorodiethylsulfide (sulphur mustard, HD) through 

polymer membrane will be presented. The gas chromatography was chosen as the 

main detection procedure and was supplied by IMS method. 

Keywords: ČSN EN ISO 6529, permeation methods, breakthrough time, 

liquid extraction, IMS detection 

1.


Lectures 

1-L04 Complex Essessment of Organophosphates 

Low Dose Chronic Exposure on Endothelium, 

Macrophages, Platelets and Esterases 

1. 

Ermolaeva E.,Goncharov N., Radilov A., Glashkina L., Mindukshev I., 

Avdonin P., Dobrylko I., Rembovskiy V. 

Research Institute of Hygiene, Occupational Pathology and Human Ecology, 

Saint-Petersburg, Russia. 

eermolaeva@mail.ru 

An attempt was undertaken to estimate a role of circulatory hypoxia, cellular 

hemostasis and neuropathy target esterase upon the exposure of organophosphates 

low doses. We studied diisopropyl fluorophosphate, which is known to be a cause 

of delayed neuropathy and paraoxon, which is known not to cause this effect. Activities 

of cholinergic and non-cholinergic esterases of rat blood plasma and brain 

were investigated, as well as generation of reactive oxygen species by peritoneal 

macrophages of mice, aggregation activity of rat platelets, and functional role of 

endothelium of rat aorta. The data obtained seem to be non-specific pointing out at 

the development of non-specific resistance under chronic exposure to organophosphates. 

At the same time, there are some qualitative and quantitative differences in 

the character of action of different organophosphates upon various components of 

the process. 

Keywords: organophophate, pathogenesis, esterases, cells


Lectures 

1-L05 Influence of Climate Conditions and Air 

Contamination on Village Inhabitants Health 


Trenčín University of A. Dubček, Department of public management, 

Študentská 2, 911 50 Trenčín, Slovakia 

mfeszterova@mail.t-com.sk 

The relation between climate conditions, atmosphere quality and health respectively 

sicknesses state and style of life in the village of Kráľovce-Krnišov located in 

South Sitno region is discussed in the paper. GIS technologies, landscape – ecology 

synthesis, questionnaire methods and statistics were used in the research. In the complex 

climate - relief was evaluated from mesoclime: inverse locations, intensity and 

direction of air flow, exposure climate, air contamination in the area – heating, agriculture 

production, transport. 

Discussing the relation between environmental health and air pollution, we 

can see several negative factors, e.g. a valley climate. Negative influence of the environment 

is seen on health state: respiratory disease 11 %. asthma 1 %, influenced by 

traffic, diseases caused by increased stress conditions of amplitude changes. Contamination 

(allergens, pesticides) may come through valley flow 15 % of inhabitants 

have dermatic problems, 18 % suffers from frequent influenza. The evaluation of 

resident climate showed the presence of stress (20 %), cordial diseases (7 %) and hypertension 

(22 %). Statistical interpretation showed increased presence of diseases 

among inhabitants aged between 41–60 years 

The village has taken part in the programme “Rural Revitalisation” and the 

project education programme at elementary school, which regards environmental 

health. 

Keywords: contamination village, air, environmental health 

1.


Lectures 

1-L06 Integration Metallomics, Proteomics and 

Transcriptomics in Environmental Issues 

1.10 

J. L. Gomez-Ariza, T. Garcia-Barrera, M. Gonzalez-Fernandez, J. Lopez-Barea 

and C. Pueyo * 

Department of Chemistry and Material Science, University of Huelva, 21007-Huelva, * Department 

of Biochemistry and Molecular, University of Cordoba, 14071-Cordoba, Spain 

Metallomics is related to metal-biomolecules characterization in tissues from 

environmental bioindicators based on multidimensional methods that combine orthogonal 

chromatographic with mass spectrometry and inductively coupled plasma 

mass spectrometry (ICP-MS). The integration of these analytical methods with the 

proteomic approach provides a powerful tool for environmental issues deciphering. 

When genetic homology between model organisms, e.g. Mus musculus, and bioindicator 

organisms, such as Mus spretus, is checked, important consequences can be 

drawn in connection to environmental metallomics, proteomics and transcriptomics.


Lectures 

1-L07 Heavy Metals in Solid Immisions in the Vicinity 

of Iron Ore Mining and Processing Plant in 

Nižná Slaná 

J. Hančuľák, E. Fedorová, O. Šestinová, 

T. Špaldon, P. Slančo, J. Brehuv 

Institute of Geotechnics of the Slovak Academy of Sciences 

Watsonova 45, 043 53 Košice, Slovak Republic 

hanculak@saske.sk 

The contribution deals with the evaluation of dust fallout monitoring, which 

was focused on heavy metals. The results from the years of 2001–2007 are introduced. 

The monitoring has been realised in the vicinity of mining and siderite processing 

plant. The siderite feed is treated by thermal technologies. Besides of gaseous 

emissions the plant also produces the solid ones compound of iron oxides and 

other heavy metals. The dust fallout was monitored by means of 17 sampling points 

located maximally up to 8 km from main source of pollution. After comparison 

with other localities, the monitored area is mostly loaded by heavy metals, which 

come from siderite feed, i.e. namely Fe, Mn and As. The highest values of deposition 

were detected on sampling points situated in the main flow of air, in the central 

part of valley. Thus, loads of individual sampling points are very different. Applied 

measuring methods and dust fallout analyses enable matter-of-fact evaluation of 

immission loading in given area. 

Keywords: heavy metals, immisions, dust fallout 

1.11


Lectures 

1-L08 Comparison of Energy Dispersive X-RAY 

Fluorescence Spectrometry, Inductively 

Coupled Plasma Optical Emission Spectrometry 

and Laser Ablation with Plasma Spectrometry 

and Laser Ablation with Plasma Spectrometry 

in the Elemental Analysis of Experimentally 

Contaminated Archive Agricultural Soils 

1.12 

Ivona Hubová 

Department of Chemistry 

Masaryk University, Brno 62500, Czech Republic 

1 @mail.muni.cz 

Methods for direct analysis are favored for the determination of total elemental 

content in agricultural soils over the solution analysis based methods, as the latter 

require the use of time consuming decomposition procedures accompanied by 

unfavourable processes such as sample loss and contamination. Usually, X-ray fluorescence 

(XRF) spectrometry belongs to methods routinely used for this purpose. 

Nowadays, laser ablation inductively coupled plasma optical/mass spectrometry 

(LA-ICP-OES/MS) still represent methods that are being investigated and gradually 

improved rather than used for reliable routine quantification. Experimentally contaminated 

agricultural soils that belong to archived materials of the Central Institute 

for Supervising and Testing in Agriculture (UKZUZ), Brno, Czech Republic were 

used to compare capabilities of energy dispersive XRF and ICP-OES of solutions obtained 

by means of decomposition with HF and HClO 4. Bioavailability of Cr, Ni, Cu, 

Zn, Pb was examined using sequential extraction procedures. Finally, LA-ICP-OES 

method was established for analysis of contaminated soils. Certified reference material 

of soil (GBW07406) was employed to verify reliability of methods. Analysis of 

pulverized samples by LA-ICP-OES/MS poses advantages and shortcoming at the 

same time. Advantages include a possibility of preparation of calibration samples 

of arbitrary composition, matrix matching and sample dissolution, while problems 

involve sometimes laborious preparation of suitable compact targets and their satisfactory 

homogeneity. 

Keywords: laser ablation inductively coupled plasma mass spectrometry, 

soil analysis


Lectures 

1-L09 Tissue-Specific Distribution and Accumulation 

of Organochlorine Pollutants in Selected 

Raptor Species from the Czech Republic 

Radim Lána, Milada Vávrová, 

Vladimír Večerek, Stanislav Kráčmar 

ICTEP, Faculty of Chemistry, Brno University of Technology 

Purkynova 118, 61200 Brno, Czech Republic 

e-mail: lana@fch.vutbr.cz 

The issue of environmental contamination by persistent organic pollutants 

(POPs) has been an object of investigation for more than three decades. Despite 

many efforts to ban or reduce their use, POPs continue to found in almost all parts of 

the global ecosystem. One of the most threatened animal species (besides humans) 

are predators, since they top the food chains within which most POPs are accumulated. 

Our comprehensive study is focused on the determination of organochlorine 

POPs (polychlorinated biphenyls and organochlorine pesticides) in the tissues of 

three raptor species from the Czech Republic. Several specimens of cormorant, grey 

heron, and common buzzard have been investigated in order to asses the distribution 

of monitored contaminants among individual tissues and potential intra- and 

interspecies differences. As for aquatic species investigated, a complex monitoring 

of the POP levels in fish and sediment samples from the specimen habitat complements 

our results which are a clear illustration of the bioaccumulation of POPs in the 

environment. Our findings are comparable to other similar studies and surveys. 

Keywords: polychlorinated biphenyls, pesticides, cormorant, buzzard, heron 

1.1


Lectures 

1-L10 CBRN Terrorism and International Legal 

Constraints 

1.1 


Masaryk University, Faculty of Sci., EU Res. Ctre of Excellence for Environ. Chemistry and 

Ecotoxicology, Kamenice 126/3, CZ-625 00 Brno, Czech Republic 

matousek@recetox.muni.cz 

End of the 20 th century was marked with shift of “classical” forms of terrorism, 

using firearms, explosives and incendiaries to CB agents, while using radionuclides 

and improvised nuclear explosive devices are expected. Worldwide community 

has been accepting legal instruments in constraining various form of terrorism 

with delay, usually after they have been executed. Existing 13 global multilateral 

conventions on combating international terrorism are thoroughly analysed starting 

with the oldest on offences on board aircraft (1963) till the newest on suppression of 

nuclear terrorism (2005) that is not yet in force. All these documents are analysed in 

association with the CBRN-terrorism. 

Antiterrorist system of UN, developed in connection with illicit trade with 

drugs, weapons, human beings, money laundering and corruption, including respective 

UN organs is characterised, relevant actions by the UN Security Council 

are stressed. 

Antiterrorist agreements and other legal documents of regional and similar 

organisations, (such as Organisation of American States. Council of Europe, European 

Union, NATO, Organisation of African Unity, Commonwealth of Independent 

States, Organisation of Islamic Conference, and like) are analysed. 

Lessons learned for the emergency preparedness and protection of population 

are presented. 

Keywords: CBRN terrorism, international legal constraints


Lectures 

1-L11 Applicability of a High Performance Chelation 

Ion Chromatographic Method to the 

Determination of Aluminium in Antarctic 

Surface Seawater 

Juliette Tria, Pavel Nesterenko, 

Andrew Bowie, Edward Butler, Paul Haddad 

ACROSS, School of Chemistry, University of Tasmania, 

Private Bag 75, Hobart, Tasmania, Australia, 7001 

jtria@utas.edu.au 

Oceanographers use surface aluminium concentrations in open-ocean seawater 

as a tracer to fingerprint the location and magnitude of atmospheric dust 

deposition. It has become increasingly important to understand the role that such 

deposition plays in supplying trace elements to surface waters and consequently the 

effects such episodic supply has on moderating biological processes. 

FIA has generally been used for the real time analysis of aluminium in surface 

seawater, however, the technique is not always robust and preconcentration is 

required. This paper presents a discussion on recent developments of a high performance 

chelation ion chromatographic (HPCIC) technique, using post column 

reaction followed by fluorescence detection, as an alternative for shipboard determination 

of aluminium. Separation of aluminium was achieved using iminodiacetic 

acid functionalised silica and fluorescence detection of the Al-lumogallion complex 

was chosen based on its excellent sensitivity. Preliminary investigation into detection 

limits for the optimised HPCIC system have shown sensitivity to be in the low 

ng L -1 region, without preconcentration and for a 20 µL sample volume. The applicability 

of the HPCIC system to the analysis of surface seawater taken from the Ross 

Sea, Antarctica was evaluated. 

Keywords: aluminium 

1.1


Lectures 

1-L12 Direct Analysis in Real Time–Time-of-Flight 

Mass Spectrometry: Analysis of Pesticides and 

Enviromental Contaminans 

1.1 

Lukáš Václavík, Jakub Schůrek, Tomáš Čajka, Jana Hajšlová 

Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, 

Department of Food Chemistry and Analysis 

Technická 5, 166 28 Prague 6 – Dejvice, Czech Republic 

lukas.vaclavik@vscht.cz 

DART (Direct Analysis in Real Time) represents a new type of ion source working 

under ambient conditions and ground potential. The sample components are 

ionised in the beam of neutral metastable species after placing the sample (liquid, 

solid, or gaseous) between the ion source and mass spectrometer. Until now, DART 

has been demonstrated to be a useful tool for rapid screening of a wide range of 

chemicals including pharmaceuticals, explosives, drugs of abuse and various natural 

compounds. 

Coupling of DART ion source to a high-resolution time-of-flight mass spectrometer 

(TOFMS) allows rapid sample characterisation. Real-time information on 

sample composition (mass spectrum) is obtained continuously; time needed for 

analysis of one sample is reduced to tens of seconds. Moreover, high-resolution 

TOFMS capable of accurate mass measurement enables analyte identity confirmation 

and identification of “unknowns” via calculation of elemental composition. 

In this presentation, the potential of DART-TOFMS system to identify/quantify 

a wide range of pesticides in various products and matrices will be demonstrated. 

The possibility to monitor some groups of environmental pollutants will be also 

discussed. 

Keywords: DART-TOFMS, pesticides, pollutants


Lectures 

1-L13 The Using of Pine-Needles, Bryophytes and 

Sponges as Bioindicators for the Evaluation of 

Persistent Organic Pollutants Environmental 

Levels 

M. Vávrová, R. Lána, M. Hroch, 

I. Hlaváčková, J. Čáslavský, B. Tremlová 

Brno University of Technology, Faculty of Chemistry; 

Purkyňova 118, 61200 Brno 

vavrova@fch.vutbr.cz 

The study submitted dealt with problems of the utilization of spruce, pine and 

fir needles, bryophytes and chosen sponges for the evaluation of ecosystems’ primary 

organic pollutants (POPs) load. Samples were being taken in several locations. 

Out of the POPs group, there were chosen organochlorine pesticides (DDT and its 

metabolites, γ-HCH and HCB), polychlorobiphenyls (PCB) and polybromodiphenylethers 

(PBDE). The isolation of tracked analytes from plant matrices was performed 

by means of extraction processes, namely via sonication and Soxhlet extraction, 

extract purification via column chromatography in the blending column, the 

very determination was performed by means of gas chromatography with μECD 

detection and the confirmation determination by means gas chromatography and 

mass detection. On the basis of results acquired, it was stated that needles contamination 

level is not negligible, the highest contamination levels showed PCB. Conifers 

and bryophytes’ results attained also lead to the conclusion that the secondary 

contamination comes from the atmosphere, which is evident namely from 100 % CB 

28 contamination; from OCHP there were dominant 4,4´-DDT and 4,4´-DDE, g-HCH 

and HCB, PBDE contamination was not so frequent and its levels were considerably 

lower than those of other POPs. In sponges, only PCB were investigated, their levels 

ranged between 1–80 µg/kg, concerning the dry matter. Based on finding attained, 

it can be claimed that bio-indicators evaluated are appropriate for POPs contamination 

level observation. 

Acknowledgement: Financial support from Ministry of Education, Youth and 

Sports under MSM 6215712402 is greatly appreciated. 

Keywords: primary organic pollutants, extraction, separation methods, 

needles, bryophytes, sponges 

1.1

1.1

Posters 

1.1

1.20


Posters 

1-P01 Ultra Trace Determination of Silver 

in Preconcentrated Water Samples by 

Electrothermal Atomic Absorption 

Spectrometry 

Seyyed Hamid Ahmadi, Javad Didevar Asl 

Chemistry & Chemical Engineering Research Center of Iran 

P.O. Box 14335-186, Tehran, Iran 

ahmadi@ccerci.ac.ir 

An efficient microextraction technique was used for detection of silver ultratrace 

quantities in water samples using the dispersive liquid–liquid microextraction 

(DLLME), followed by the electrothermal atomic absorption spectrometry (ETAAS). 

In this technique, a mixture containing ethanol (disperser solvent), CCl 4 (extraction 

solvent) and sodium diethyldithiocarbamate(chelating agent), was rapidly injected 

by a syringe into the sample, spiked with silver. The silver ions reacted with the 

chelating agent and were extracted into the fine droplets of CCl 4 . After centrifugation, 

the fine CCl 4 droplets sedimented at the bottom of the conical test tube. The 

concentration of enriched analyte in the sedimented phase, was determined by 

ETAAS. Effective parameters such as the type and the volume of the solvents, the 

extraction time, the salt effect, pH and the quantity of the chelating agent were optimized. 

Under the optimum conditions, the enrichment factor of 150 was obtained 

from only a 5.00 mL water sample. The calibration graph was linear in the range of 

0.05–1 μg L −1 with the detection limit of 0.02 μg L −1 . The silver recoveries in natural 

water samples at the spiking level of 0.20 and 0.40 μg L −1 varied from 94.5 to 103.0. 

The main DLLME advantages combined with ETAAS were simplicity of operation, 

rapidity, low cost, high-enrichment factor and good repeatability. 

Keywords: silver, wateranalysis 

1.21


Posters 

1-P02 Removal of 2-mercaptobenzothiazole 

from Synthetic Wastewater 

1.22 

Beáta Almásiová, Ján Derco, 

Angelika Kassai 

Slovak University of Technology, Faculty of Chemical and Food Technology, 

Institute of Chemical and Environmental Engineering, 

Radlinského 9, 812 37 Bratislava 1, Slovak Republic 

beata.almasiova@stuba.sk 

2-Mercaptobenzothiazole (MBT) is the most important member of the benzothiazole 

group of heterocyclic aromatic compounds. This xenobiotic compound is 

used mainly in the manufacture of rubber additive chemicals but also has other 

uses, notably as a corrosion inhibitor. It is known as a widespread, toxic and poorly 

biodegradable pollutant. It is able to pass conventional treatment systems, widespread 

with surface and underground waters and enters into organisms. 

We were studied degradation of 2-mercaptobenzothiazole with ozone. The feasibility 

of utilisation of ozonation process for reduction of concentration of MBT 

was investigated in laboratory scale equipment. The experiments were performed 

in bubble ozonation column. The process efficiency was considerably influenced 

by time of ozonation. The content of MBT in synthetic wastewater containing MBT 

decreased more than 5 times after 15 minutes of ozonation performed at 70 % of the 

maximum power of ozone generator. Total removal of 2-MBT from the sample was 

achieved by ozonation. 

Respirometric tests confirmed that this compound is not biodegradable. At the 

concentration above 100 mg/l it can inhibit demand of biodegradable compounds. 

MBT is not consumed by non adapted activated sludge. Partial decay of MBT by 

microorganism was observed with adapted activated sludge within 8 days. The adsorption 

tests confirmed the ability of MBT to adsorb on surface of activated sludge. 

This ability of MBT depends significantly on the type of activated sludge. 

Keywords: adsorption, biodegradability, 2-mercaptobenzothiazole, ozonation


Posters 

1-P03 Health Risk Assessment by Indoor Air Quality 

Monitoring 

Ekaterina Andreeva, Ivan Mašek, Milada Vávrová 

Brno University of Technology, ICTEP, Czech Republic 


xcandreeva@fch.vutbr.cz 

Today the chemical substances are used during any kind of work, thus creating 

certain chemical risks at many workplaces all over the world. Their quantity is being 

estimated by few of thousands, which is permanently growing. 

Therefore the system approach for predicting risks from work with dangerous 

substances and following safety rules are matters of high priority. One of the elements, 

the quality of indoor air, was chosen. The purpose of this study is to develop 

the system of steps for health risk assessment at workplaces in university chemical 

laboratories. 

In particular, the aim of the study is the group of the priority organic pollutants 

in the environment – volatile organic compounds, especially mostly used in the 

laboratory different kinds of solvents (hexane, dichloromethane, acetone, acetonitrile, 

ethanol etc.). 

According to some sources, they act not only as central nervous system depressants, 

but also affect the human reproduction. 

The different working areas (laboratory of organic chemistry, environment matrix 

sampling, personal sampling) are chosen. In these areas measurement of concentration 

of the solvents are conducting by means of diffusive sampling system 

Radiello with following GC with FID or GC-MS analysis. 

Keywords: workplace safety, dangerous substance, indoor air 

1.2


Posters 

1-P04 Nitrogen in breeding laying hens 

and environment protection 

1.2 

Mária Angelovičová, Marek Angelovič, Miroslava Kačániová, 

Daniela Liptaiová, Kamil Močár, Dávid Štofan 

Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, 

Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, 

maria.angelovicova@uniag.sk 

High nutrient balances exert pressures on the environment in terms of an 

increased risk of leaching of nitrates to groundwater. 

We realized experiments with laying hens, which ingested feed mixture 

with different protein content. It is soya cereal type assigned for laying hens. 

The laying hens ingested fodder ad libitum. 

Within experiments were researched: protein contents in feed mixture in one 

kg, excreted nitrogen (N) in dropping per bird and day (chemical analysis – Kjeldahl 

method and calculation). 

After ingestion of feed mixture with protein content 173.10 g per kg was N 

content in dropping from 1.80 to 1.95 g per bird and day. The laying hens, which 

fed feed mixture with protein contents 146.12 or 146.68 g per kg excreted the N in 

dropping in range 1.35 to 1.40 g per bird and day. From these results follow that near 

decrease protein contents in feed mixture from 173.10 to 146.12 or 146.68 g per kg is 

possibility of N decrease in excreted dropping at laying hens about 25.0 to 28.21% 

per bird and day. 

The research results about N in breeding of laying hens in relationship to environment 

protection confirmed the possibility its decrease. One of the possibilities is 

decrease of protein contents in feed mixture. With decrease of protein content 

in feed mixture from 173.10 into 146.68 or 146.12 g per kg (about 15.26 to 15.59%) is 

possibility of decrease of excreted N in dropping at laying hens about 25 to 28.21%. 

Keywords: nitrogen, dropping, layers, feed mixture, protein


Posters 

1-P05 Testing of Various Sorbents for Copper Removal 

from Acid Mine Drainage 

Magdaléna Bálintová, Natália Kovaliková 

Civil Engineering Faculty, Technical University of Košice, 

Institute of Building and Environmental Engineering, 

Vysokoškolská 4, 042 00 Košice 

magdalena.balintova@tuke.sk 

Elimination of the consequences of mining activities belongs to the most serious 

environmental problems nowadays. Acid mine drainage (AMD) with high 

metal concentrations and usually with low value of the pH (about 2–4) is mainly 

a result of chemical oxidation of sulphides and other chemical processes in overflowed 

mines, mining waste dumps and tailings. This water may transfer various 

heavy metals in a dissolved form, such as Fe, Cu, Al. 

The paper deals with utilization of four types sorbents (zeolite, active carbon, 

turf brush PEATSORB and universal crushed sorbent ECO-DRY) for coper removal 

from AMD Smolník and presents their influence on Cu decreasing under various 

conditions. The change of pH has been monitored, too. All sorbents slightly decreased 

pH (pH AMD was 3,8) that was positive fact because pH above 4 is connected 

with precipitation of heavy metals. 

Keywords: acid mine drainage, copper, sorbents 

1.2


Posters 

1-P06 Influence of Composite Sorbent for Selected 

Elements Content in the Sediment Load of a 

Water Reservoir and Mine Sludge Basin 

1.2 

Ján Brehuv, Oľga Šestinová, Tomislav Špaldon, 

Pavel Slančo, Jozef Hančuľák, Erika Fedorová 

Istitute of Geotechnics of Slovak Academy of Sciences, 

Watsonova 45, 045 53 Ko3ice, Slovak Republik 

E-mail:brehuv@saske.sk 

The paper contains information about results of the laboratory experiments. 

They were focused on decrease of content of selected contaminants (heavy metals) 

in sediment load of water reservoir and mine sludge basin samples. For experiments 

the composite sorbent was used, which contains natural materials with sorption attributes. 

The first results of influence of this sorbent on heavy metals content in the 

investigated (examined) samples were obtained. On this base the experiments will 

continue. 

Keywords: sediment load, water reservoir, sludge basin, composite sorbent,


Posters 

1-P07 Monitoring of Different Trace Metals Species 

Concentration in Morava River 

Lukáš Brulík, Pavel Diviš, Hana Dočekalová and Jiří Machát 

Brno University of Technology, Fakulty of Chemistry 

Purkynova 118, Brno, CZ-61200, Czech Republic 

brulik@fch.vutbr.cz 

This work summarized results from an experiment in Morava River (Czech 

Republic, South Moravia) running during the September 2007 and December 2007. 

The concentrations of Cd, Cu, Ni, Pb were monitored using different sampling techniques. 

Kinetically labile species were measured by DGT (diffusive gradient in thin 

films technique), total dissolved and total metal concentrations were monitored using 

the conventional sampling techniques. The bioavailable concentrations of trace 

metals were monitored by application of moss bags with Fontinalis Antipyretica 

aquatic moss species. 

Keywords: Fontinalis Antipyretica, DGT, heavy metals 

1.2


Posters 

1-P08 Volatile Degradation Products of Polyurethane 

Foams 

1.2 

Josef Čáslavský, Daniela Mácová, Tereza Tobiášová 



xcmacova @fch.vutbr.cz 

Polyurethanes are widely used in many industrial branches. Most of their production 

represents flexible polyurethane foams. They are also often deposited on 

waste dumps, where they degrade under the influence of various environmental 

factors; hydrolysis and photolysis are the main degradation mechanisms. 

Polyurethane foams were modified by addition of biodegradable filler to enhance 

their degradation ability. This foam was photo-degraded by UV irradiation. 

Volatile photodegradation products were the subjects of analysis. These compounds 

were adsorbed by Solid Phase Microextraction (SPME) using polyacrylate 

and polydimethylsiloxane fibers. Gas chromatography with mass spectrometry was 

the method of their final analysis. 

Keywords: polyuretane foam, volatile degradation products

1-P09 English fro Chemists Can Be pHun 

Gabriela Clemensová 


Purkynova 118, Brno, CZ-61200, Czech Republic 

clemensova@fch.vutbr.cz 


Posters 

Is it necessary to teach English for specific purposes? Is good knowledge of 

general English enough one needs to be able to communicate? Foreign language 

competence can be defined as the ability of authentic self presentation in that language. 

Accepting this approach to competency we can say that English for specific 

purposes has its irreplaceable position in a postsecondary curriculum. 

Our mission of teaching English for future chemists is more or less challenged 

by 3 major phenomena: 

1. Absence of appropriate teaching materials on the market 

2. The different language experience of the students who come to our school. 

3. The absolute majority of our students strongly oppose and almost detest memory 

based learning as they are used almost entirely to rely on their ability of 

logical reasoning 

4. Last but not least challenge is that the technically oriented students are not such 

good “natural speakers”. even in their mother language as the students of the 

humanities. 

The paper describes the approach to these above mentioned issues. Special emphasize 

is put on the preparation and use of appropriate web based material which 

enables to bring the Internet into the classes. 

Keywords: English for specific purposes, material preparation, Internet 

supported language learning 

1.2


Posters 

1-P10 Dynamic Simulation of Biological Nitrogen 

Removal Processes 

1. 0 

Lenka Černochová, Ján Derco 

Faculty of Chemical and Food Technology, Institute of Chemical 

and Environmental Engineering, 

Slovak University of Technology in Bratislava 

Radlinského 9, 812 37 Bratislava, Slovakia 

lenkacernochova@stuba.sk 

The Activated Sludge Model No. 1 (ASM1) was used for modelling of biological 

stage of real wastewater treatment plant (WWTP). Two series of diurnal variation 

measurements of wastewater flow and composition in input and output at 

biological stage of real WWTP were carried out. The first set of measurements was 

performed before the reconstruction of WWTP (year 2002) and the second one after 

the reconstruction (year 2007). The results of measurements were transformed into 

organic and nitrogen pollution fractions according to ASM1 concept and the database 

structure of the used simulation program (SIMBA). 

In this work is shown utilization of simulation program SIMBA in which is included 

ASM1 to describe behaviour of WWTP. Dynamic simulations were realized 

for different operational states. Results of dynamic simulations show quite good 

agreement between experimental and calculated. Mathematical model was used for 

verification of steady state design and for prediction effluent concentration values 

as well. 

Keywords: biological wastewater treatment plant, Activated Sludge Model 

No. 1, dynamic simulations, prediction, simulation program


Posters 

1-P11 Application of a 6-Mercaptopurine 

Functionalised Sorbent for Diffusive Gradients 

in Films Technique 

Pavel Diviš and Roman Szkandera 


Purkynova 118, Brno, CZ-61200, The Czech Republic 

divis@fch.vutbr.cz 

Iontosorb AV a modified bead-form celulose sorbent containing aminoaryl-ßethylsulfone 

groups was functionalised by coupling it through an azo group with 

6-mercaptopurine. The resulting sorbent was characterised and tested for use in 

diffusive gradient in thin films technique. 

Keywords: resin, mercury, DGT 

1. 1


Posters 

1-P12 The Determination of Methylmercury in Water 

Ecosystems 

1. 2 

H. Doležalová Weissmannová, J. Čáslavský, M. Vávrová, 

L. Tuhovčáková 

Brno University of Ttechnology, Faculty of Chemistry 

Purkyňova 118, Brno, Czech Republic 

dolezalova@fch.vutbr.cz 

Environmental contamination by mercury compounds is clearly a growing 

problem even today. Mercury is among the most highly bioconcentrated toxic trace 

metals in the organisms. Mercury toxicity depends on its chemical form, among 

which alkyl mercury compounds are the most toxic. Methylmercury is well known 

neurotoxic agents for human and animals. The concern about methylmercury is 

based on its effects on ecosystems and human health and alkyl mercury has received 

considerable attention because of the toxicity of methylmercury compounds 

(MeHg), the accumulation of Hg in biota, and its biomagnification in aquatic food 

chains. By reasons of the seriousness of methylmercury the determination is growing 

need for analytical and separation methods. The determination of methylmercury 

in samples from water ecosystems is not easy; the very low concentrations 

together with the presence of interfering substances (such as sulphides, humic acids, 

amino acids, and proteins) able to strongly bind methylmercury are the main 

analytical complications. The main steps to determination of methylmercury need 

extraction, separation and mercury-specific detection, for these purpose the conventional 

technique can be used (GC-ECD, GC-MS, HPLC). 

Keywords: methylmercury, chromatography


Posters 

1-P13 Ecotoxicological Testing and Test Methods of 

Chemicals and Waste 

H. Doležalová Weissmannová, H. Zlámalová Gargošová 

Brno University of Ttechnology, Faculty of Chemistry 


dolezalova@fch.vutbr.cz 

Ecotoxicology belongs to one of the new sciences which emerged as a consequence 

of the adverse effects of technology on complex natural systems. The main 

purpose of toxicological and ecotoxicological testing is the determination of doseeffect. 

Ecotoxicological testing involves the prediction of the impact of very low 

concentration of a chemical or leached waste. In aquatic ecotoxicological testing the 

many organisms can be used (Algae, Crustacea). The standard ecotoxicological tests 

with aquatic organisms are used for definition and description of environmental 

effects of chemicals, since most of the pollutants are found in the water. The aim of 

this contribution is the comparison of the standard test (OECD) and determination 

of sensitivity of testing organism for standard chemical. 

Keywords: ecotoxicity, standard OECD tests 

1.


Posters 

1-P14 Examining Indoor Aerosols 

1. 

Adriana Eštoková, Nadežda Števulová, Lenka Kubincová 

Technical University of Košice, Civil Engineering Faculty, 


Vysokoškolská 4, 042 00 Košice, Slovak Republic 

Adriana.Estokova@tuke.sk, 

This work is primarily concerned with PM 10 concentrations level and settled 

particulate matter in indoor environment assessment as well as with the chemical 

composition, size and shape of particles investigation. 

The indoor particulate matter concentration in various types of residential and 

non-residential buildings were monitored and compared with Slovak indoor PM 10 

limit. The highest mass concentration of PM 10 was observed in non-residential public 

buildings. The concentrations of deposited particles on horizontal surfaces were 

measured in residential buildings at various high levels. The trend of gradual decreasing 

of particulate matter occurrence with the raise of height was observed. 

The collected dust samples were characterized by scanning electron microscopy/energy-dispersive 

X-ray analysis (SEM/EDX). The individual particles in irregular 

shape of the various particle size as well as aggregates of ultra-dispersion 

particles were observed on the SEM micrographs. The majority of dust particles are 

non-spherical in shape with strong division of the surface. The occurrence of the 

spherical particles as well as of fibrous particles was not obvious. Elemental analysis 

was carried out by energy-dispersion x-ray spectroscopy. The trace metal contents 

were investigated by atomic absorption spectroscopy. The high mass concentrations 

of heavy metals were determined. The indoor concentrations of magnesium and 

calcium because of former ambient source existence were investigated too. 

Keywords: PM10, dust


Posters 

1-P15 Atmospherical Deposition and Imission Situation 

in the Nižná Slaná Area 

Erika Fedorová, Jozef Hančuľák, Oľga Šestinová, 

Ján Brehuv, Tomislav Špaldon 

Institute of Geotechnics of the Slovak Academy of Sciences, 

Watsonova 45, 043 53 Košice, 

fedorova@saske.sk 

The Siderit plant Nižná Slaná is exploiting the iron ore – siderit with the local 

deposit. The plant is interesting with air pollution from different sources. 

The biggest of this sources is chimney which is 120 m high. The dust fallout 

was monitored by means of 17 sampling points located maximally up to 8 km from 

main source of pollution. The pollutants have municipal as well as industrial origin. 

The range of plant emission by chemical data processing and by their comparision 

with dust deposition was investigated. Finally, the imission action of the plant on its 

wider surroundings was found out by this way. 

In the present time the communal and the natural sphere causes the air pollution. 

The main are line and area sources. 

Key words: atmospherical deposition, imission and emission situation, dust, 

solid air pollutants 

1.


Posters 

1-P16 The Natural and Anthropological Sources of 

the Halciansky Water Reservoir Contamination 

1. 


Trenčín University of A. Dubček, Department of public management, 

Študentská 2, 911 50 Trenčín, Slovakia 

mfeszterova@mail.t-com.sk 

The aim of the paper is to identify natural and anthropological sources of contamination 

within the model area of Halčiansky water reservoir, which is part of 

Belianske reservoirs basin in Banská Štiavnica region. Theoretical scopes such as relief, 

geological, climate and soil conditions, fauna, flora, were deliberated. Samples 

from six sites taken in terrain (in 2006–2007) were analysed spectrophotometrically 

(HACH DR 2000, Horiba). Natural and anthropological contamination sources were 

processed by basic GIS software (Geomedia professional, Microstation, Idrisi). 

The results showed: pH, conductivity, turbidity, temperature, salinity meet the 

– –1 limits of the state norm No. 491/2002 in codex. The content of NO (0,07–0,43 m gl ) 

3 

exceeded the limit, especially at sites close to surrounding meadows, fields and 

– –1 3– pasturelands. The NO concentration (0,004–0,212 mg l ) and PO4 concentration 

2 

(0,7–1,8 mg l –1 ) exceeded the norm limits during the whole research period at all 

2– studied sites. The SO concentration didn’t exceed norm limits. CHSKCr measures 

4 

didn’t meet norm limits at all sites during the research period (20–2800 mg.l –1 ). 

Lixiviums from organically reach soils, forest and moss, pesticides, fertilizers, 

field dung-hills, pasture-land, grasslands, illegal cesspit waste from residential 

zones, sewage waters from lodge and cabin areas as well as illegal dumping 

grounds were identified as the main contamination sources. 

Keywords: contamination, water reservoir, GIS software


Posters 

1-P17 Biomodified Forms of Zeolites and their 

Environmental Application 

Ľubica Fortunová, Mária Reháková, Silvia Čuvanová, Lucia Gaberová, 

Mária Kušnierová 

Faculty of Science, P. J. Šafárik University, 

Moyzesova 11, 041 54 Košice 

maria.rehakova@upjs.sk 

Natural zeolite of clinoptilolite type from East Slovakian deposit in Nižný 

Hrabovec has been studied with respect to its feasibility of application in environmental 

area in combination with biotechnological methods for decreasing the residual 

content of heavy metals and other toxic compounds in industrial contaminated 

soils. 

The aim of the study was enlarging of clinoptilolite sorption surface by effect 

of microorganisms Thiobacillus ferrooxidans and obtaining more effective results of 

its application in the process of the reduction of the content of toxic substance residua 

in industrially contaminated soils. By using the microorganisms the changes of 

the surface, total volume of the cavities and bioextraction on model forms of natural 

clinoptilolite with a content of some heavy metals was confirmed. 

Thiobacillus ferrooxidans is used in mineral technologies in the process of extraction 

of Cu, Fe and other metals that are present in soil in low concentrations. The 

combination of the natural zeolites and bioremediated technologies is perspective 

environmental alternation of decrease the residua content of the toxic substance in 

heavily contaminated soils in industrial areas. 

Keywords: zeolite, clinoptilolite, heavy metals, bioremediation 

1.


Posters 

1-P18 Distribution Patterns of Organic Pollutants 

in Brno Lake with Respect to Its Depositional 

History 

1. 

Eva Francu, Jan Schwarzbauer, Mathias Ricking, 

Radim Lána, Pavel Muller, Juraj Francu 

Czech Geological Survey, Leitnerova 22, 658 69 Brno, 

eva.francu@geology.cz 

Brno Lake is situated on the Svratka River and belongs to the most important 

reservoirs in the Southern Moravia region. A pilot study focused on the occurrence 

of risk elements and organic pollutants in vertical profiles of the sediment layers has 

been carried out. A Th-U(Ra)-K gamma-spectrometric survey showed that a probable 

source of these elements is in the weathered granitic material of the Brno Massif 

with negligible contribution from the U-mines. The sediment dating using Cs 137 

indicates the position of the 1986 1961–2. Analyses of 1–3 m deep probes reveal the 

distribution patterns of organic carbon, sulphur, PAHs and PCBs, and provide a tentative 

pattern related to depositional history and flow rate. The geochemical profiles 

show a general upward increase in eutrophycation and organic matter accumulation 

associated with increased cyanobacterial blooms. The sum of nine PAHs often 

exceeds the 10 mg/kg EPA limit value and their pyrogenic character suggests an 

origin in low-quality fuel and furnaces used in the villages and recreational facilities 

in the watershed. PCBs with dominating congeners 138, 153 and 180, on the other 

hand, show upward increase with some stagnation during last decade. The integration 

of the environmental geochemical archive into the sedimentological restoration 

of depositional and erosional sequence of events provides a new dynamic view of 

the pollution in historical terms. 

Keywords: depositional history, organic pollutants


Posters 

1-P19 New Resin Gel for Diffusive Gradients in Thin 

Film (DGT) Technique 

M. Gregušová, B. Dočekal, H. Dočekalová 

Department of Environmental Chemistry and Technology, Faculty of Chemistry, 

Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic 

xcgregusova@fch.vutbr.cz 

The diffusive gradients in thin film technique (DGT) represent a relatively new 

approach to in situ determination of labile metal-species in aquatic systems. The 

DGT device accumulates labile species from the solution while deployed in situ and 

therefore contamination problems associated with conventional collection and filtration 

procedures are eliminated. The technique employs a hydrogel layer to control 

the diffusive transport of metals to a cation-exchange resin, usually Chelex 100, 

which is selective in free or weakly complexed metal ion species. 

This study deals with a new resin gel based on Spheron-Oxin® (5-sulphophenyl-azo-8-hydroxychinoline) 

ion exchanger for DGT technique. This new binding 

phase with a higher selectivity for trace metal species than Chelex 100 could potentially 

provide more information on metals speciation in aquatic systems. The performance 

of this new binding phase was tested for the determination of Cd, Cu, Ni, 

Pb and U under laboratory conditions. Resin gel based on Spheron-Oxin® appears 

to be a new useful resin gel for speciation analysis of uranium in natural waters. 

Acknowledgement: This work was performed and supported within the Institutional 

research plan AV0Z40310501. 

Keywords: DGT technique, speciation analysis 

1.


Posters 

1-P20 Extending of the Scope of GC/MS Method for 

Multiple pesticide Residues: Validation Study 

1. 0 

Markéta Hakenová, Jana Urbanová, 

Ondřej Lacina, Vladimír Kocourek, Jana Hajšlová 

Institute of chemical technology Prague, Department of Food Chemistry and Analysis, 

Technická 5, 166 28, Prague 6 Dejvice, Czech Republic 

hakenovm@vscht.cz 

Hundreds of pesticides have been registred for protection of food crops during 

their growth and storage. Multiresidue methods are used to control maximum 

limits established for particular pesticide/comodity combination. In our laboratory 

method of gas chromatography in connection with mass spectrometry detection is 

employed for determination of over 100 residues occurring in “pesticide fraction“ 

obtained by gel permeation chromatographic (GPC) clean-up of crude ethylacetate 

extract. 

The aim of the current study was to extend the scope of this method by adding 

20 pesticides (2-phenylphenol, ametryn, cadusafos, cyanazine, cyromazine, desmetryn, 

dicrotophos, fluoxastrobin, fonofos, metoxuron, metazachlor, monuron, pencycuron, 

prometon, pyrazophos, pyrethrins, simetryn, tau-Fluvalinate, thiometon 

a vamidathion) on the list of the target analytes. The performance characteristics 

obtained by validation of optimised method will by critically assessed with regard 

to EU requirements on analytical methods. 

This project was funded by National Agency for Agriculture Research (NAZV) 

QH81292. 

Keywords: pesticides, gas chromatography


Posters 

1-P21 Challenges in the Analysis of 

Hexabromocyclododecane (HBCD) in the 

Environmental Samples 

Petra Hrádková, Jan Poustka, Jana Pulkrabová, 

Michaela Nápravníková, Jana Hajšlová 

Department of Food Chemistry and Analysis, ICT Prague 

Technická 3, CZ-16628 Prague 6, Czech Republic 

e-mail address: petra.hradkova@vscht.cz 

HBCD has been widely used as an additive flame retardant in various materials. 

There are theoretically 16 HBCD stereoisomers, however, the commercial 

technical mixture consist mainly of three diastereomeric pairs of enantiomers (α-, 

β-, and γ-HBCD), the latter one is the most abundant. Some difficulties may be observed 

in the determination and quantification of HBCD in the samples, hence accurate 

detection techniques are required. Individual HBCD diasteromers can be determined 

using LC/MS-MS (ESI), whereas total-HBCD (sum of α-, β-, and γ-HBCD) 

may be also measured by GC/MS (NCI). GC/MS does not allow the quantification 

of the individual isomers, because the diastereoisomers interconvert at temperature 

above 160 °C and the insufficient chromatographic and spectral separation. On 

the other hand, the response in LC/MS-MS system may be influenced by ion suppression 

especially in the electrospray ionisation MS. In this study, the both types 

of analytical systems were tested to assess the quality of HBCD determination in 

the environmental samples. Our results are in accordance with those published in 

similar studies concerned with biotransformation processes of the isomers profile 

resulting in dominance of α-HBCD. 

This is study was undertaken within the projects MSM 6046137305 and NPV II 

(2B06151). 

Keywords: HBCD; GC/MS; LC/MS 

1. 1


Posters 

1-P22 The Content of Polybrominated Diphenyl Ethers 

in Freshwater Fishes from Brno Water Reservoir 

1. 2 

M. Hroch , M. Vávrová , V. Večerek 

Faculty of Chemistry, University of Technology Brno 

Purkyňova 118, Brno 612 00, Czech Republic 

hroch@fch.vutbr.cz 

Most attention has been focused on the group of polybrominated diphenyl 

ethers (PBDEs), which are widely used as flame retardants in polymers for textiles, 

electronics and home furnishings and they more likely leach out and transfer into 

the environment and biota. As a result, PBDEs have been detected in biotic and 

abiotic matrices including sediments, air, water, fish, marine mammals, human 

plasma, and human milk. This paper presents results of measuring concentration of 

ten PBDE congeners (BDE-3, 15, 28, 47, 99, 100, 118, 153, 154 and 183) in fishes skin 

and muscles (amur, bream, carp, crucian) by using gas chromatography with electron 

captured detector (GC-ECD). These samples were collected from Brno water 

reservoir, which is located on north-west near Brno city. 

Five head of each kind were detected and the total PBDE congener concentrations 

(Σ PBDEs) in the fishes were amur < bream < crucian < carp. Residues of main 

congeners were not found in all samples. The highest concentrations were identified 

in carp skin BDE-47 (26 do 92 µg.kg -1 ) and crucian musles BDE-153 (31–106 µg.kg -1 ). 

Congeners BDE-15, 28 and 118 were not identified in all of analyzed samples. 

Keywords: flame retardant, GC-ECD


Posters 

1-P23 Examination of Aband)oned Sb Deposits 

by Mineralogical and Geochemical Methods 

in Poproč (Slovakia) 

Michal Jankulár, Tomáš Klimko, Michal Šutriepka, Marek Vaculík 

Faculty of Natural Sciences Comenius University 

Mlynska dolina, 84215 Bratislava, Slovak Republic 

jankular@fns.uniba.sk 

Surrounding of abandoned Sb-deposits in SE of Spišsko-gemerské Rudohorie 

Mts. (Slovakia) poses environmental large-scale contamination by toxic elements 

(e.g. Sb, Pb, As, Cu, Zn) as a result of long-term mining activity, which finished in 

second half of 20 th century. The main scientific interest is focused on migration of 

toxic elements in environment, their bindings on Fe-oxyhydroxides or other natural 

sorbents, possibility of input to food item and potentially influence on population 

health quality. 

Antimonite is main ore mineral of mineralization occurring as quartz-carbonate 

veinlets, other ore minerals are pyrite, arsenopyrite and other Pb-Sb-Zn-Cu sulphides. 

In investigated area field research and sampling of geological materials and 

natural media (groundwater, surface waters, stream sediments, soils, ochre) and biological 

samples (selected plant species) were realized by standard methods. Sample 

localization was focused on surrounding of potential local contamination sources 

(outflow from mines, tailings ponds and theirs surrounding, mining wastes). Material 

of ochre is potential contamination source of toxic elements for aquatic environment 

in catchments of Olšava and Bodva rivers. Further experiments will bring 

important data for bioavailability assessment. 

Keywords: Sb-deposits, toxic elements 

Keywords: soil type, fractions of sulphure, sulphur dynamics 

1.


Posters 

1-P24 Determination of Volatile Compounds and 

Saccharides at Alder Wood Hydrolysis 

1. 

František Kačík, Marta Laurová, Danica Kačíková 

Technical University in Zvolen 

T. G. Masaryka 24, 960 53 Zvolen, Slovakia 

kacik@vsld.tuzvo.sk 

Hydrolysis of fast-growing wood species is an important way to production of 

saccharides from renewable resources. Resulting sugars can be converted to ethanol 

by fermentation. 

In this study, formation of volatile compounds (methanol, 2-furaldehyde, acetic 

and propionic acids) as well as saccharides during hydrolysis of alder wood at 

160-200 °C has been studied by gas chromatography. At the mild conditions of hydrolysis 

the acetic acid and 2-furaldehyde concentration increases due to the saccharides 

deacetylation and dehydration, respectively. The amount of 2-furaldehyde 

is decreased at 200 °C due to its participation in the condensation reactions. The 

maximum of both monosaccharides and total saccharides contents were found at 

180 °C and 60 minutes of treatment. 

Keywords: hydrolysis, saccharides, alder wood


Posters 

1-P25 Determination of Methylmercury in Sediments 

by Headspace Gas Chromatography and Atomic 

Fluorescence Detection after Aqueous Phase 

Ethylation with Sodium Tetraethylborate 

Milada Kadlecová, Baghdad Ouddane, Hana Dočekalová, 

Jean-Claude Fischer 


Brno University of Technology, 


xckadlecova@fch.vutbr.cz 

Aqueous phase ethylation, headspace injection (HS), separation by gas chromatography 

(GC) with cold vapour atomic fluorescence detection (CV-AFS) is widely 

used technique for analysis of mercury, because of its accuracy and high sensitivity. 

The purpose of the present work was to verify and optimise the parameters for determination 

of methylmercury (MeHg) in sediments. 

The analytical system for determination methylmercury consists of a headspace 

sampler (Perkin Elmer HS TurboMatrix-40 Trap) coupled to a GC (Perkin Elmer, 

Clarus 500) through a heated fused silica transfer line. The transfer line is directly 

connected to the packed GC capillary column consists of a Teflon tube filled with 

10% OV3 on Chromosorb. The outlet of GC is coupled to a CV-AFS (Tekran, Model 

2600 Mercury Analysis System) via a pyrolysis unit. The pyrolysis oven (Varian, 

ETC-60 Electrothermal temperature Controler) was provided with a temperature 

control set. 

The method for methylmercury determination was based on the work of Leermakers 

et al. (Anal Bioanal Chem, 2003) For MeHg extracted samples were ethylated 

and directly introduced to GC column by HS or injected after trapped (pre-concentrated) 

on Tenax columns and separated in the GC. Both technique were tested 

and compared. The better recovery of methylmercury determination and also better 

detection limit is by using HS with Trap instead of HS only. 

The optimization of extraction procedure (the time agitation, time for contraextraction), 

ethylation parameters (volume of ethylating agent, pH, reaction time), 

headspace parameters (volume sample, thermostating time, HS temperature, pressurization 

time, injection time) and gas chromatography parameters (column temperature, 

carrier gas flow rate) are presented. The initial systems values... 

The extend of this abstract exceeded the allowed size and therefore had to be clipped. 

1.


Posters 

1-P26 Modeling of Dispersion of Windborne Material 

in Atmosphere 

1. 

Michal Kapoun, Radim Dvořák 

Brno University of Technology, Faculty of Chemistry 

Purkyňova 118, 612 00 Brno 

xckapoun@ch.vutbr.cz 

This paper deals with mathematical modeling of dispersion of windborne 

material that serves as the basic assumption to create software to predict dispersion 

in environment during chemical accidents. As an example a point source of pollution 

in atmosphere with defined meteorological conditions was chosen. 

Keywords: modeling, dispersion, atmosphere


Posters 

1-P27 Reprocessing of Dangerous put out Chemicals 

and Wastes 

Juraj Kizlink 

Faculty of Chemistry, Brno University of Technology 

Purkyňova 118., CZ-612 00 Brno 

kizlink@fch.vutbr.cz 

The possibilities of reprocessing of some dangerous put out chemicals and wastes 

are described. Benzidine by the means of diazotization and boiling in methanol 

into 4,´4-dimethoxydiphenyl (fragrance). Hydrazine by means of boiling with 

benzaldehyde into substituted hydrazone, known under trade name Benzalazine 

(cheap UV-absorber). Alkaline cyanides by means of reaction with benzylchloride 

into benzylcyanide and after hydrolysis in ethanol, followed by azeotropic distillation 

is possible to obtain aromatic phenylacetic acid ethyl ester. After reduction by 

means of hydrides it is possible to obtain 2-phenylethanol (fragrance). The chemical 

substitute of natural rose oil. 

Key words: dangerous chemicals, dangerous wastes, waste reprocessing, 

chemical fragrances, 

1.


Posters 

1-P28 Application of Chitosan by Water Treatment 

1. 

Zuzana Klímová 


Purkyňova 118, 61200 Brno, Czech Republic 

klimova-z@fch.vutr.cz 

Chitosan is a derivative of chitin, a polysaccharide that is the major component 

of the shells of crustaceans and insects. Chitin consists of long chains of acetylated 

D-glucosamine, that is, glucosamine with acetyl groups on the amino groups (Nacetylglucosamine). 

Chitosan is N-deacetylated chitin, although the deacetylation 

in most chitosan preparations is not complete. The protonization of amino groups in 

solution makes chitosan positively charged, and there by very attractive for flocculation 

and different kinds of binding applications. Since most natural colloidal particles, 

including bacteria and macromolecules, are negatively charged, attractive 

electrostatic interactions may lead to flocculation. 

This study deals with treatment of surface water with content of humic matters 

with the help of coagulation. It was worked with coagulants, ferrous and clayey sulphate, 

that are in practice commonly used and with natural biopolymer on chitosan 

base, that is not used CR. The aim of this work was to found optimal use of chitosan 

and to compare its coagulative effectivity with effectivity of coagulation of standard 

coagulants ferrous and clayey sulphate. 

Keywords: chitosan, coagulation, humic substances, water treatment


Posters 

1-P29 Substituted Carboxylic Acids as a Guest for 

Cucurbiturils 

Viktor Kolman, Vladimír Šindelář 

Masaryk University, Fakulty of science 

Kotlářská 2 , 625 00 Brno 

sindelar@chemi.muni.cz 

Recent studies approved, that suitably substituted carboxylic acids can create 

the supramolecular complexes with cucurbit[n]urils (CB[n]), which behave as 

molecular switches. At low pH these complexes are stable but the decomplexation 

takes place in basic environment. 

Our work is based on the synthesis of dipyridinium salts bearing carboxylic 

group in central aliphatic/aromatic unit. We studied these compounds as a guest 

for the supramolecular interaction with various CB[n] macrocycles in order to prepare 

pseudorotaxane switches. In these systems carboxylic group can be hidden 

in the CB[n] cavity and exposed to the water environment after the carboxylate is 

formed. 

Determination of stability constants of the synthesized guests with macrocyclic 

host CB[n] is presented using 1 H NMR and UV-VIS spectroscopy. Finally, we 

describe switching properties of the pseudorotaxane system, which is controlled by 

pH of the solution. 

Keywords: cucurbituril, supramolecular switch 

1.


Posters 

1-P30 The Effect of Waste Basalt Wools on the 

Chemical, Agrochemical, Pedological and 

Hygienic-Toxicological Soil Parameters 

1. 0 

Peter Kováčik, Alena Vollmannová, Jaroslav Noskovič 

Department of Agrochemistry, Slovak University of Agriculture in Nitra 

Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic 

Peter.Kovacik@uniag.sk 

The effect of two waste rock wools (Nobasyp and Agrodrap) on the soil parameters 

has been studied in the pot trial realized in a vegetation cage located at the 

Slovak Agricultural University in Nitra ( 48° 18´ N, 18° 05´ E) on Haplic Luvisol (25 

kg soil per pot). 

Achieved results have shown that application of Agrodrap or Nobasyp 

(20 t.ha –1 ) has statistically significantly increased the cation exchange capacity, the 

sum of exchange basic cations (chiefly Mg, K, Ca) and the base of saturation of the 

soil. The bulk density of the soil has been decreased significantly as well, whereas 

Nobasyp has determined the parameters more importantly than Agrodrap. 

Both materials have had a positive effect on the total carbon content and the organic 

matter quality, thereby have increased the humic acid share in the soil, whereas 

Agrodrap has had more significant effect on these parameters than Nobasyp. 

Both waste rock wools have had an alcalic effect on the soil and have deadened 

acidic effect of the fertilizers and the negative effect on the increase of salt content in 

the soil as well. They have had the same effect on the content of 9 determined heavy 

metals in the soil (Cd,Pb,Cr,Zn,Cu,Co,Ni,Mn,Fe) as NPK fertilizers application. 

Due to the neutral effect of wools on the heavy metals content in the soil and the 

positive effect on the remaining soil parameters can be Nobasyp and Agrodrap regarded 

as soil amendments. 

Keywords: waste basalt wools, soil amendments


Posters 

1-P31 Influence of Water Erosion Processes on the 

Bottom Sediment Quality 

Natália Kovaliková, Magdaléna Bálintová 

Civil Engineering Faculty, Technical University of Košice, 



natalia.kovalikova@tuke.sk 

Erosion processes in watersheds belong to serious ecological and economical 

problems. Sediments, originated by water erosion reduce stream capacity and cause 

silting of water basins. Additionally, large amounts of chemicals (such as heavy metals, 

nutrients, and other pollutants) adsorbed to soil particles can be transported 

via soil erosion to water bodies that result in water pollution problems. Especially 

nutrients are the important pollution source, which is often attributed to non-point 

source pollution from agricultural production areas. 

This contribution deals with the nutrient transport from eroding upland fields 

by the small water basin Kľušov. 

Our research has been focused on monitoring the nutrient concentration (nitrogen, 

phosphorus) in soil particles detached from hillslopes during the soil erosion 

and the granularity impact of soil particles on the nutrient concentration. The 

results from chemical analyses confirmed the literary information, that the concentrations 

of P, N in chosen sediment samples were diverse due to irregular sediment 

deposit in the reservoir. The next part of our study deals with determination of the 

nutrient concentration in sediment and soils in dependence on application quantity 

and type of used fertilizers and the crop uptake rates. 

Keywords: erosion, nutrients, sediment quality 

1. 1


Posters 

1-P32 Modelling And Diagnosing of Mechanical 

Engineering Life Cycle Production Process 

1. 2 

Ružena Králiková, Alena Paulíková 

Department of Environmental Studies, SjF - TU in Košice, 

Park Komenského 5, 041 87 Košice, Slovakia 

ruzena.kralikova@tuke.sk, 

Competing business challenges to interest in production process as determined 

factor for quality products, environment protection, production machinery condition, 

required parameters for machining and elaborateness for given accuracy. One 

of solution is the implementation modern simulating devices and applications of 

analogs in production practice. 

Keywords: LCA, simulation


Posters 

1-P33 Simulation Of Chemical Factors in Working 

Environment 

Ružena Králiková, Alena Pauliková 

Department of Environmental Studies, SjF - TU in Košice, 

Park Komenského 5, 041 87 Košice, Slovakia 

ruzena.kralikova@tuke.sk, 

The contribution deals of the modelling and the influences of selected chemical 

factors in working environment. It is applied by means of the modern simulation 

of software devices. The methodology with the look-up functions of simulation 

program Vensim was designed for complex assessment of working environment in 

specific conditions of mechanical engineering enterprises. 

Keywords: chemical factors, working environment 

1.


Posters 

1-P34 Multi-Residue Method for the Analysis of 

Pesticides and Mycotoxins in Cereals 

by LC-MS/MS 

1. 

Ondřej Lacina, Jana Urbanová, Alexandra Krplová, Lukáš Václavík, 

Jana Hajšlová and Jan Poustka 

Institute of Chemical Technology Prague 

Technická 5, 166 28 Prague 6 

ondrej.lacina@vscht.cz 

In recent decade, liquid chromatography–tandem mass spectrometry (LC-MS/ 

MS) operated in selected-reaction-monitoring mode, has become the main tool for 

the analysis of food and environmental contaminants presenting wide range of 

physico-chemical properties. This approach allowed the introduction of multiresidue 

methods with up hundreds target analytes determined in a single run. However, 

most of these methods are focused only on one group of food contaminants 

such as pesticides, veterinary drugs, mycotoxins, plant toxins, etc. 

Considering the fact, that sample preparation/detection principles are basically 

similar for most of these methods, we attempted to determine pesticide residues 

and mycotoxins - contaminants representing different sources of origin in single 

run. The comprehensive LC-MS/MS multi-residue method has been developed and 

validated for 12 Fusarium toxines and approximately 200 pesticides, that may occurred 

in cereals. 

Keywords: liquid chromatography, tandem mass spectrometry, pesticides, 

mycotoxins


Posters 

1-P35 Aerobic MTBE Biodegradation by Paecilomyces 

Variotii 

Blazo Lalevic,Vera Raicevic, Ljubinko Jovanovic, Dragan Kikovic, 

Miomir Niksic 

Faculty of Agriculture of Belgrade University 

Nemanjina 6, 11080 Belgrade - Zemun 

lalevicb@yahoo.com 

Methyl tert-butyl ether (MTBE) has been used since the 1970s as a gasoline additive 

in order to octane replacement for tetraethyl lead, increasing of combustion 

efficiency and reduction of carbon monoxide and VOC emission. However, the widespread 

use of MTBE in different types of gasoline, has resulted in its introduction 

into groundwaters. The MTBE has led to increased need to identify and aplicate the 

cost-effective bioremediation technologies. The aim of this paper was investigation 

of MTBE biodegradation by fungus Paecilomyces variotii isolate 129b, isolated from 

the wastewater of API separator in Oil Refinery of Pancevo district. The experiment 

was conducted by inoculation of liquid MSM medium with spores of Paecilomyces 

variotii. The MTBE was added in following concentrations: 11; 34; and 83 ppm. The 

MTBE concentration were detected using GC/FID method. The leveling of degradation 

rate was conducted in the beginning of experiment and after 2; 5; 12; 15; and 19 

days from the beginning of the experiment. 

The results indicate that Paecilomyces variotii can degrade MTBE. The degradation 

rate was from 41–85%, amount of fungi biomass was from 0.017–0.021 mg/ml 

depending on starting MTBE concentration and the time of sampling. This studies 

confirms the possibilities of fungus Paecilomyces variotii in degradation of recalcitrant 

pollutants such as MTBE. 

Keywords: MTBE, biodegradation, Paecilomyces variotii 

1.


Posters 

1-P36 Reclamation of Calcium Sulphate Sludges by 

Sulphate-Reducing Bacteria 

1. 

Alena Luptáková, Mária Kušnierová 

Institute of Geotechnics of Slovak Academy of Sciences 

Watsonova 45, 043 53 Kosice, Slovak Republic 

luptakal@saske.sk 

Combustion of fossil fuels containing sulphur releases sulphur oxides to the 

atmosphere. If combustion gases are desulphurized by lime or limestone scrubbing, 

calcium sulphates sludges are generated and these must be disposed of. Under appropriate 

conditions these sulphate can be converted to sulphide by bacterial sulphate 

reduction as hydrogen sulphide gas, which can be oxidized chemically or 

biologically to elemental sulphur. Because bacterial sulphate reduction by sulphatereducing 

bacteria has widespread environmental effects and this process has potential 

as a treatment process for calcium sulphate sludge and acid mine drainage, 

the aim of this work was to study the gypsum biodegradation from the final stored 

product – “stabilizate“, from deposit thermal power plant Vojany in Slovakia. The 

process may consist of three stages: anaerobic sulphate reduction to hydrogen sulphide 

by sulphate-reducing bacteria, metals precipitation from acid mine drainage 

by continuous stripping of hydrogen sulphide and the excess hydrogen sulphide 

oxidation to elemental sulphur. Metal sulphides, calcium carbonate and elemental 

sulphur are final products and these may by recycled in chemical or hydrometallurgical 

processes. 

Keywords: calcium sulphates sludges, sulphate-reducing bacteria


Posters 

1-P37 Bioleaching of Antimony Minerals 

by Bacteria Acidithiobacillus and Desulfovibrio 

Alena Luptáková, Eva Mačingová, Jana Jenčárová 


Watsonova 45, 043 53 Kosice, Slovak Republic 

luptakal@saske.sk 

The objectives of this work were to evaluate the use of sulphate-reducing bacteria 

Desulfovibrio sp. and sulphur-oxidising bacteria Acidithiobacillus sp. for the 

leaching of gold-bearing antimony sulfide minerals. The basic metabolic process 

of Desulfovibrio sp. is the anaerobic reduction of sulphates to hydrogen sulphide, 

which can use for leaching antimony-bearing materials. The basic metabolic process 

of Acidithiobacillus sp. is the aerobic oxidation of sulphur in result of which may be 

used for the oxidation of sulphur from antimony sulphide minerals. 

Series of aerobic and anaerobic tests were studied in a fed batch reactor at 30°C. 

Samples of gold-bearing antimony sulphide minerals were kept in static and dynamic 

conditions. After 40 days the solid and liquid phase were analysed. Solubilized 

metals were determined using an atomic absorption spectrophotometer. Solid 

residues recovered by filtration were analysed by means of X-ray diffraction. 

Keywords: bioleaching, sulphate-reducing bacteria 

1.


Posters 

1-P38 Biological-Chemical regeneration of 

Desulphurization Sorbents Based on Zinc Ferrite 

1. 

Alena Luptáková, Mária Kušnierová, Vladimír Šepelák 

Institute of Geotechnics SAS, 

Watsonova 45,040 01 Košice, Slovak Republic 

kusnier@saske.sk 

Nowadays is the zinc ferrite used as an adsorption material for high-temperature 

desulphurization of waste gases after coal combustion. 

The first results of the experiments using the biological-chemical way of leaching, 

on principle a new process of regeneration of desulphurization sorbents 

based on zinc ferrite is shown in this paper. 

Keywords: zinc ferrite, bioregeneration, leaching


Posters 

1-P39 Single Analyte Zone Splitting in CZE: Origin, 

Appearance and Remedy 

Z. Malá, P. Gebauer, P. Boček 

Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, v. v. i. 

Veveří 97, 602 00 Brno, Czech Republic 

mala@iach.cz 

It is not a rare phenomenon in capillary zone electrophoresis (CZE) that the 

electrophoregram shows more peaks than is the number of components present in 

the sample. In analytical practice, these redundant peaks are often left unexplained 

or are declared as unidentified impurities or system peaks. So far, there has been 

paid little attention to a case when one single analyte can form two or more regular, 

mutually separated, distinct zones. This contribution brings the results of theoretical 

and experimental investigation of the electromigration behavior of salt-containing 

samples in various currently used background electrolytes (BGEs), and, it is 

shown that this type of samples can exhibit splitting of the single analyte zone into 

mutually independent parts that can be detected as several well developed distinct 

peaks. Theory based on velocity diagrams and computer simulations reveals that 

the origin of these effects lies in the transient phase of sample separation. Here, the 

(salt containing) sample induces parallel existence of several transient sharp boundaries 

simultaneously capable to stack an analyte. The electromigration of these 

boundaries is convergent, however, if these boundaries do not merge before the 

analyte destacks from them, permanent double or even multiple peaks of the given 

analyte are the result. Presented examples of anionic and cationic systems show 

good agreement between theory/simulation and experiment and demonstrate that 

the appearance of multiple peaks can be very variable, ranging from double or triple 

peaks to a major peak with a minor peak quite apart. Knowledge of the mechanism 

of the peak splitting phenomenon allows both to identify its presence in a given 

BGE/sample system and to find effective remedy. 

Acknowledgements: This work was supported by the Grant Agency of the 

Czech republic, grant No 203/08/1536, by the Grant Agency of the Academy of 

Science of the Czech Republic, grants No IAA 400310609 and IAA 400310703, and 

by the Institutional Research Plan AV0Z40310501 of the Academy of Sciences of the 

Czech Republic. 

Keywords: capillary zone electrophoresis, peak spliting 

1.


Posters 

1-P40 Chlorophyll Bleaching by UV-Irradiation 

in Solvents and Isolated Photosynthetic 

Organelles 

1. 0 

Dejan Marković, Jelena Zvezdanović 

Faculty of Technology, University of Niš 

16000 Leskovac, Serbia 

dejan_markovic @yahoo.com 

Solar radiation is not only the biosphere vital source of energy, but also acts as 

an adverse environmental factor for various forms of life. The light-induced damage 

increases with decreasing wavelengths. Thus, from natural sunlight that reaches the 

Earth the UV-B portion (280-315 nm), in increasing proportions with recent ozone 

layer reductions, has the highest damaging potential. 

Chlorophyll (Chl), a major photosynthesis pigment, is a chlorin, porphyrin 

derivative, a cyclic tetrapyrrol with isocyclic cyclopentanone ring, fused at the edge 

of the right-bottom pyrrole ring; with central Mg-atom playing a coordinating role. 

Though it primarily absorbs in VIS region, it‘s composure is significantly altered 

when exposed to UV-light in vivo and in vitro. 

Chlorophyll bleaching by UV-irradiation (UV-A, UV-B and UV-C) was studied 

by absorbance and fluorescence spectroscopy in acetone and n-hexane extracts of 

photosynthetic pigments, and in thylakoids suspensions. Chlorophyll undergoes 

bleaching accompanied by fluorescent transient formation obeying the first-order 

kinetics. The bleaching is governed by UV-photons energy input, as well as by different 

Chl molecular organizations in the two solvents (in vitro), and in thylakoids 

(in situ). UV-C-induced bleaching of chlorophyll in thylakoids is probably caused by 

different mechanism, compared to UV-A and UV-B induced bleaching. 

Keywords: chlorophyll, UV-irradiation, bleaching, thylakoids


Posters 

1-P41 Environmental Risk Assessment, Transport of 

Dangerous Goods and Possible Accidents 

Jana Victoria Martincová and Ivan Mašek 



platina@email.cz 

Road transport of dangerous goods is increasing and there are also increasing 

numbers of registered accidents. The environmental risk assessment is possible to 

approach by means of methods applicable in releases and dispersion modelling of 

dangerous gases or vapours and other dangerous goods in cases of major accidents 

from road transportation in urban zones and landscapes. The most transported 

dangerous goods on the roads are flammable substances. The description for the 

hazardous transport corresponds to the European ADR Directive. There will be predictions 

in the article from the software ALOHA and TeRex used and the numerical 

methods such as IAEA TEC DOC 727 and Dow´s FαE Index. 

Keywords: environmental risk asssessment, transport of dangerous goods, 

accidents 

1. 1


Posters 

1-P42 Causes of Accidents Regarging Transport of 

Dangerous Goods 

1. 2 

J. V. Martincová, I. Mašek, J. Martinec 



platina@email.cz 

A Transport of dangerous substances can be characterized by a permanent contact 

or mutual conjunction with the population and represents in all possible stages 

a potential hazard. In addition to human health injury or death, also damage to 

property and the affected environment remains a very serious problem. Considering 

each of the negative aspects relating to hazardous materials transportation the 

increase of 95 % in numbers of road accidents have been recorded in the last 30 years 

and must be accounted. This negative feature of the excessive traffic on roads is in 

evidence due to available statistics of the accidents in the whole Europe. Besides 

the noticeable number of accidents in the cases related to the transport of dangerous 

substances the economical point of view in increasing costs is under general 

concern. 

The most frequented transportation regarding dangerous goods is on the roads 

in the Europe. 

Keywords: dangerous goods, road transport, statistics, causes of accidents.


Posters 

1-P43 Development of a new Educational Programme 

“Protection of Population” 

Ivan Mašek, Pavel Častulík, , Otakar Mika 

Brno University of Technology,Chemistry and Technology of Environmental Protection 


masek@fch.vutbr.cz 

Authors in their contribution inform about newly conceived master degree 

educational programme, its scope of the study, profile of graduates, orientation of 

study and theoretical disciplines including practical training and composition of 

study subjects. Further they present the views and suggestions on the profile of a 

new study field and its technological orientation, with respect for education of graduates 

in complexity of arrangements for protection of population against hazardous 

materials such as chemicals, in particular. In this contribution will be outlined 

the possibilities for cooperation of the University and/or the Faculty of chemistry 

with public administration and their associated institutions, research and development 

establishments and manufacturing companies in order to provide protection 

of population through crisis management and technological support. 

1.


Posters 

1-P44 Can we Ensure Safer Environmental 

for Curtural Heritage? 

1. 

Ivan Mašek, Zdena Rosická 

Brno University of Technology, Faculty of Chemistry, 

Purkynova 118, Brno, 612 00, Czech Republic, 


In the late 70ies it was estimated that only 10% of the cultural heritage in Europe 

would survive by 2000. The principal threats were identified as development, 

traffic and road construction. The causes of decay of the cultural heritage are in 

different degrees common to most countries in the world: climatic causes (floods, 

extreme frosts, wildfire…), geological causes (ground water, mining settlement…), 

economic causes (lack of maintenance, development…), biological causes (moulds, 

fungi, lichens, rats, pigeons and man as well), and man-made causes (pollution, fire, 

arson, neglect, vandalism, theft…). 

Library and archive materials call for specific environmental conditions necessary 

to maintain the collections safely in a stable condition. HVAC systems, relative 

humidity, temperature, pollutants in the air, light, monitoring equipment and 

methods, mold and mildew, insects, vermin and fumigation - all elements of environmental 

(EC) conservation are in question. EC is a preventive preservation program 

for the entire collection and therefore is a more cost effective in the long run 

than individual treatment of library materials.


Posters 

1-P45 Risks Factors of Social Development for 

Management 

Ivan Mašek, Jaromír Novák 




The article deals with some of the main risks factors of social development 

both at present time and in future. The authors characterise phenomena and processes 

taking part in society. These phenomena and processes can gain risky characters, 

having negative influence on further development. It is necessary to respect 

these factors both in theory and practice even at lowers levels of management. These 

factors include: population increase, world’s wealth, globalisation, integration and 

disintegration, weapons, violence, permanent development, and ability to manage. 

Key words: Society, development, risks factors, population increase, 

world’s wealth, globalisation, integration and disintegration, 

weapons, violence, permanent development, ability to manage. 

1.


Posters 

1-P46 Actual Problems of chemical Disarmanet: 

Chemical Weapons Convention after the 2nd 

Review Conference 

1. 


Masaryk University, Facultty of Sci , 

EU Res. Ctre of Excellence for Environmental Chemistry and Ecotoxicology, Kamenice 126/3, 

CZ-625 00 Brno, Czech Republic 

matousek@recetox.muni.cz 

The Chemical Weapons Convention (CWC) is characterised and its special 

mood of defining prohibitions and commitments of States Parties (SPs), based on its 

leading principle, the s.c. General Purpose Criterion, stressed. 

The CWC is the only functioning document on eliminating one kind of weapons 

of mass destruction (WMD) under effective international verification system. 

Status of its implementation 11 years after entry into force is assessed, mainly as 

of the course and problems of destruction of CW stockpiles and destruction/conversion 

of former CW production facilities. Fundamental problems of non-proliferation, 

i.e. mainly of non-production of CW in peaceful chemical industries and 

verification thereof, are elucidated. 

Impact of advances in science and technology on the CWC and its implementation 

is analysed vis-à-vis the assessment of achievements of the 1 st and 2 nd Review 

Conferences (2003, 2008). Special attention is devoted to experience in combating 

chemical terrorism, assistance and protection, and national implementation measures. 

Experience of implementing CWC in the Czech Republic and its contribution 

to the worldwide chemical disarmament is discussed. 

Keywords: Chemical Weapons Convention, implementation


Posters 

1-P47 Protection of Population in The Czech Republic 

and its Perspectives 

Otakar J. Mika, Lenka Fišerová 


Institute of Chemistry and Technology of Environmental Protection 

Purkyňova Street No. 464/118, CZ-612 00 Brno, Czech Republic 

mika@fch.vutbr.cz 

Brief survey of civil protection and defence history in the former Czechoslovakia. 

Civil protection and protection of population in the Czech Republic after sociopolitical 

changes at the beginning of 90-ties. Current state of protection of population 

in the Czech Republic and perspectives of its development in the forthcoming 

years. 

Present requirements on modern system of protection of population with respect 

to all significant risks and hazards. Outline proposal of a new legal regulation 

(Civilian Protection Act) for both current situation and following 10-15 years. 

Keywords: protection of citizen, emergency situations, Civilian Protection Act 

1.


Posters 

1-P48 Application of DGT Method for Assesment of 

Availability of Heavy Metals to Plants 

1. 

Z. Mládková, K. Pešková, B. Dočekal, H. Dočekalová, P. Škarpa 



xcpeskova@fch.vutbr.cz 

The content of heavy metals in agricultural soils is gradually increasing from 

various processes such as utilization of fertilizers, atmospheric fall-out etc. These 

metals may transfer from contaminated soils into growing plants. Therefore, it is 

essential to assess the biological relevance of heavy metals in soils. Various leaching 

procedures are applied for this purpose. Total mineral analysis procedures can not 

provide information on biochemical activity of heavy metals. Sequential extraction 

procedures are adopted for determination of heavy metals distribution in different 

forms and phases. Diffusive gradient in thin films (DGT) can be applied for characterization 

of soils. DGT probe, when deployed in the soil disturbs soil/pore water 

equilibrium and simultaneously measures the response of the soil, resupply fluxes 

of labile metal species from the soil solid phase to soil solution. 

The results of DGT and extraction techniques by common leaching procedures 

are presented and compared with the metals uptake in various parts of an experimental 

plant (Raphanus) cultivated on tested soils. 

Acknowledgement: This work was performed and supported within the Institutional 

research plan AV0Z40310501. 

Keywords: DGT technique, analysis of soils


Posters 

1-P49 Spectrophotometric Microdetermination of 

Phosphate Based on the Ion Association Complex 

with Rhodamine B in Water 

Martin Moos, Lumír Sommer 



xcmoos@fch.vutbr.cz 

The spectrophotometric determination of phosphate based on the ion associa- 

tion komplex with Rhodamin B is H 2 [P(Mo 3 O 10 ) 4 ] ө RhB ⊕ . Linear calibration curves 

were observed for 1–7⋅10-6 - –5 3 -1 M H PO (∆λmax = 552 nm, ∆ε = 1.42 × 10 dm mol cm ) in 

2 4 

1 M sulphuric acid in the presence of 0,01% non-ionic surfactant Brij 35 and 0,03 M 

α β sodium molybdate. The detection limits are X = 12.4 ppb XD = 36.6 ppb (Graham), 

D 

m XD = 24.4 ppb (Miller) or 25.6 ppb (IUPAC). No interferences at 1 × 10–4 – M H PO 2 4 

were observed for 1000 : 1 Mg2+ , K + , Na + + – 2– – – 3+ , NH , NO3 SO4 , Cl , HCO3 and 100 : 1 Al , 

4 , 

Fe2+ , Ca2+ – . As (III, V), NO and Si (IV) were especially tested as considerable interfe- 

2 

– rents, from which As (III, V) and NO interfere above the concentration only which 

2 

cannot be found in natural waters. The Si (IV) was masked with 0.1 g l –1 tartaric 

acid. The optimalised method was applied fot the determination of phosphate in 

drinking and surface water. 

Keywords: phosphate, Rhodamin B, water 

1.


Posters 

1-P50 Determination of Uranium by ICP-AES in the 

Absence and Presence of Preconcentration on 

Macroporous Sorbents 

1. 0 

Martin Moos, Urbánková Kristýna, Lumír Sommer 



xcmoos@fch.vutbr.cz 

The echelle based ICP spectrometer at 27.12 MHz, with power output 1.35 kW, 

Meinhard concentric nebuliser and CID detector is used for the determination of 

U. Linear calibration plots for 0.1–50 ppm U(IV) are in solutions containing 1–3 % 

HNO for 385.958 and 409.014 nm. The slope of the plot decreases with the increasing 

3 

concentration of HNO but increases in the presence of 4-(2-pyridylazo)resorcinol 

3 

(PAR) by 10 %. 3 × 10 –3 M surfactants such as Septonex, Brij 35 and dodecylsulphate 

considerably increase the sensitivity of the determination of U. The detection limits 

α β m 

are X = 1.26, XD =3.69 (Graham), XD = 1.13 (Miller) or 0.30 ppm (IUPAC). No interfer- 

D 

– – 2– 2– + ences at 1–3 ppm U were observed for 100 : 1 NO , Cl , SO4 , SiO3 , NH4, 

Na, K, Ca, 

3 

Mg, Al 10 : 1 multicomponent containing Br – , Cl, Br – 2– 3– , SO , PO4 and Cd, Co, Cr, Cu, 

4 

Mn, Pb, V, Zn in the absence or presence of internal standard Y (1ppm) except of Fe3+ for which the interfering signal 10 : 1 excess decreases only by 50 % in the presence 

of Y. The macroporous Amberlite XAD 4 conditioned with the cationic surfactant in 

the presence of PAR has proved for the preconcentration of U at pH 5–9. The subsequent 

elution is with mixtures of aceton, ethanol or methanol and 1–4 M HNO . The 3. 

solvent is removed by evaporation prior to the ICP determination. 

Keywords: uranium, amberlite XAD, ICP-AES


Posters 

1-P51 Determination of halogenated Pollutants in 

Environmental Samples by using GC-ECD after 

SPME-GC Separation 

F. Moreno, J. M. Manso, T. García-Barrera, J. L. Gómez-Ariza 

Dpto. de Química y CC.MM. Facultad de Ciencias Experimentales. Universidad de Huelva. 

Campus de El Carmen. 21007 Huelva (SPAIN). 

tamara.garcia@dqcm.uhu.es 

The presence of halogenated pollutants in the environmenta has a great concern, 

especially chlorinated pesticides and chlorinated or brominated biphenyls. 

Their introduction in the food chain that can promote hazard effects on the animals 

and men and the legislation tends to provide guidelines levels to avoid consequences 

for health. Special interest has been focused on fruit and vegetables to reduce to 

reduce the maximum residue levels (MRLs). As a consequence, accurate determinations 

reducing the analysis costs are needed. 

A number of methods have been proposed for determination of these POPs 

in environmental and food samples, but the task is still the development of new 

analytical methods allowing the extraction from the complex matrixes, elimination 

of interfering co-extracted compounds and detection limits under the MRLs. In addition, 

the determination of PCBs and PBBs is a difficult task due to the similarity 

of these compounds which normally coeluted in the chromatographic separations. 

Thus, a powerful analytical approach for halogenated POPs is based on the use of 

GC-ECD/MS. This coupling is simple and transferable to routine laboratories. 

In this work, SPME-GC-ECD/MS has been applied to halogenated compounds 

in environmental and food samples avoiding coelution problems. A surface response 

design (Rotable Central Composite Design-RCCD) was constructed in order 

to obtain the optimum experimental conditions. 

1. 1


Posters 

1-P52 Simultaneous Speciation of Selenium and 

Mercury in Biological Samples by using a Column 

Swithching Systém with Liquid Chromatography 

Coupled to ICP-MS 

1. 2 

F. Moreno, T. García-Barrera, J. L. Gómez-Ariza 


Campus de El Carmen. 21007 Huelva (SPAIN) 

tamara@dqcm.uhu.es 

It has been issued that the simultaneous intake of selenium and mercury prevents 

against some neurotoxic effects of mercury. For this reason, analytical methods 

for the multispeciation of elements are required. However, is difficult to obtain 

optimal instrumental parameters for both elements as well as the establishment of 

common extraction conditions. In the present study, a new method for the simultaneous 

extraction of Se- and Hg-species has been developed. The optimized analytical 

strategy for the simultaneous extraction of Se- and Hg-species was based on the 

use of an ultrasonic probe and a microwave oven as well as solid-liquid extraction. 

The analyzed species: Se-methionine, Se-cystamine, Se-cystine, Se-(methyl)-Se-cysteine, 

Se (IV), Se (VI), Se-urea, methylmercury and inorganic mercury, showed good 

recoveries with the proposed method. The separation of the species was carried out 

with two columns connected to a column switching system coupled to an ICP-MS 

that allowed the separation of all the analytes in less than 25 minutes. 

The method has been validated by using several certified materials, namely: 

SELM-1 (selenium enriched yeast), DORM-2 (dogfish), TORT-2 (lobster hepatopancreas), 

IAEA-407 (fish homogenate) and IAEA-85 (human hair)


Posters 

1-P53 Volatile Organic Substances Present in Spices 

and Spruce Needles 

Ludmila Mravcová, Milada Vávrová, Josef Čáslavský, Michaela Stoupalová, 

Ilona Hlaváčková, Hana Vítečková 

Vysoké učení technické v Brně, Fakulta chemická 


mravcova@fch.vutbr.cz 

The study concentrates on the identification of selected content substances 

present in spices and in spruce needles. The attention was given to volatile organic 

substances of the group of aromatic oils. The isolation and identification of the mentioned 

substances was carried out by two ways. The first way used the extraction 

for the isolation of monitored analytes from the matrix and thin layer chromatography 

with chemical detection for the identification. This is the method recommended 

by the Pharmaceutical Codex as a screening method. The second way was based on 

passive sampling using SPME and identification of analytes using GC/MS. The study 

is closed with evaluation of the both methods for detection and identification of 

content substances present in aromatic oils isolated from spices or spruce needles . 

Keywords: GC/MS, SPME, aromatic oils 

1.


Posters 

1-P54 Changes in Carotenoids Pattern in Mougeotia 

sp. Algae Induced By High Light Stress 

1. 

Edward Muntean, Victor Bercea, Nicoleta Muntean, Nicolae Dragoş 

University of Agricultural Sciences and Veterinary Medicine Cluj Napoca 

3-5 Calea Mănăştur, 400372 – Cluj Napoca, Romania 

edimuntean@yahoo.com 

The behavior of the photoprotective function of xanthophyll cycle was studied 

using as matrix the green algae Mougeotia sp. Agardt, using excessive light. For investigating 

the light-dependent adaptation of the violaxanthin cycle pigments, the 

carotenoid analysis was achieved using high performance liquid chromatography 

(HPLC) with photodiode array detection. Separations were performed on an Agilent 

1100 system, using a Nucleosil 120-5 C 18 column (250 x 4.6 mm) and the following 

mobile phases: A - acetonitrile : water (9 : 1) and B - ethyl acetate. The flow rate 

of the mobile phase was 1 mL/ min. and the solvent gradient was as follows: from 0 

to 20 min. - 10% to 70% B, then from 20 to 30 min. - 70% to 10% B. 

HPLC analysis revealed a carotenoid pattern in which the xanthophylls violaxanthin, 

lutein, zeaxanthin, 5,6-epoxy-β-carotene and the carotenes α-carotene, 

β-carotene, 9Z-β-carotene and 15Z-β-carotene were identified. This pattern is dominated 

by two major carotenoids: lutein and β-carotene. HPLC analysis also revealed 

a particular behavior of xanthophyll cycle in Mougeotia sp. algae under intense illumination: 

the major de-epoxidation product is not zeaxanthin, but antheraxanthin. 

Besides, the light stress affects the whole carotenoid biosynthesis and thus the 

HPLC carotenoid pattern, starting with tηε ϖιολαξαντηιν χψχλε σ πρεχυρσορ: βcarotene. 

Keywords: HPLC, carotenoids


Posters 

1-P55 Simultaneous Ion Chromatographic 

Determination of Anions and Cations in Surface 

Waters From Fizes Valley 

Muntean Edward, Mihăiescu Tania, 

Muntean Nicoleta, Mihăiescu Radu 

University of Agricultural Sciences and Veterinary Medicine Cluj Napoca 

3-5 Calea Mănăştur, 400372 – Cluj Napoca, Romania 

edimuntean@yahoo.com 

The system of fishing ponds, streams and ground waters from Fizes Valley watershed 

(Romania) was studied in order to assess the effects of antropic pollution 

through leaching of fertilizers from soils and waste waters from the villages within 

its catchment. An ion chromatography (IC) method with conductivity detection was 

+ , 

developed, enabling a simultaneous determination of six cations (Li + , Na + , K + , NH4 Mg2+ and Ca2+ ) and seven anions (F- , Cl- , Br- - - 3- , NO , NO3 , PO4 and SO4 

2 

2- ) in a single 

run, saving thus analytical time, sample pre-treatment and reagents. 

IC separations were performed on a Shimadzu system equipped with two 

Alltech columns in parallel: an Allsep Anion 7u column (100 x 4.6 mm) and an 

Universal Aation 7u (150 x 4.6 mm) column - both maintained at 400C. Anion separations 

were accomplished using as mobile phase 4-hidroxybenzoic acid 4 mM with 

pH-ul adjusted to 7.5 with LiOH 0.1 M, at a flow rate of 0.85 mL/ min; cation separations 

were carried out using as mobile phase HNO 3 mM with EDTA 0.1 mM at a 

3 

flow rate of 0.5 ml/min. Aliquots of 250 µl samples were used for all determinations. 

All peaks were baseline separated, the total separation time being 23 min. The obtained 

data were validated using certified reference materials (LGC6020, SPS ww2); 

all calibrations were linear, with r>0.99. Only eight ions were found in the samples 

+ 2+ 2+ - - 3- 2- , Mg , Ca , Cl , NO3 , PO4 and SO4 ), within a concentration range of 

(Na + , K + , NH4 6.64 ppm (for K + ) to 423.27 ppm (for Cl- ). 

Keywords: ion chromatography, surface water 

1.


Posters 

1-P56 Strategies to Reduce Detection Limits (LODs) 

in the Analysis of Brominated Flame Retardants 

(BFRs) in Environmental Samples 

1. 

Michaela Nápravníková, Jana Pulkrabová 

Petra Hrádková, Jana Hajšlová, Jan Poustka, Dita Svobodová 

Department of Food Chemistry and Analysis, ICT Prague 

Technická 3, 16628 Prague 6, Czech Republic 

michaela.napravnikovascht.cz 

BFRs, represented by polybrominated diphenyl ethers (PBDEs), have become 

in recent years of a great concern because of endocrine disruption and other potential 

health adverse effects. Now a days, these highly persistent and bioaccumulative 

chemicals have been monitored in a wide range of environmental compartments 

(both biotic and abiotic) including humans. Due to very low concentrations of PB- 

DEs in these types of samples, which are approximately 10 – 100 times lower compared 

to levels of major halogenated persistent organic pollutants (POPs) – polychlorinated 

biphenyls (PCBs). Low detection limits (LODs) of respective analytical 

method are required. The potential of following modification of GC-MS procedure 

is presented in this study: (i) replacement of electron impact ionization (EI-MS) by 

negative chemical ionization (NCI-MS), (ii) the use of programmable temperature 

vaporization (PTV) instead of commonly used splitless injection and (iii) the use 

of comprehensive two-dimensional (orthogonal) gas chromatography coupled to 

time-of-flight mass spectrometry (GC×GC–TOF MS). 

This is study was undertaken within the projects MSM 6046137305 and NPV 

II (2B06151) both supported by the Ministry of Education, Youth and Sports of the 

Czech Republic. 

Keywords: PBDE; PTV; GC-MS-EI; GC-MS-NCI

1-P57 Chemicals and Energy Form Biomass 

Ľudovít Polívka, Eva Ürgeová 

Department of Biotechnologies, Faculty of Natural Sciences, 

University of St. Cyril and Methodius in Trnava, 

Nám. J. Herdu 2, 917 01 Trnava, Slovakia; 

ludovit.polivka@ucm.sk 


Posters 

Biomass as renewable sources is very important feedstock for biotechnological 

production food and non-food products. The chemical industry has used biotechnological 

processes for many years using natural and genetic modified biosystems 

form last two decades. 

Application a modern biotechnology including green biotechnology some recent 

reports predict production of 10% of all chemicals by the year 2010 and fine 

chemicals products or segments including chemical “building block” by the same 

year. Biotechnologies are the basic pillar of SUSCHEM and others European technological 

platforms. 

The survey of very important chemicals and pharmaceuticals products are referred. 

In connections with utilisation of biomass as a source of energy and chemicals, 

the new aspects of technology and products of biorafinery are presented. 

Keywords: biomass, chemical products, bioenergy, fine chemicals, biorafinery 

1.


Posters 

1-P58 Simultaneous Determination of Mercury, Lead 

and Cadmium in Aqueous Samples Using Picric 

Acid-Modified Carbon Paste Electrode 

1. 

Jose H. Santos, Sean Gerard Ward 

Department of Chemistry, University of Brunei Darussalam 

Tungku Link, Gadong BE141, Negara Brunei Darussalam 

joey@fos.ubd.edu.bn 

An environmentally friendly alternative to mercury electrodes for the analysis 

of heavy metals by anodic stripping voltammetry (ASV) is presented in this paper. 

Picric acid isolated from orange peel was utilized as a modifier for carbon paste electrode 

(CPE) and used as a working electrode in the ASV of mercury, lead and cadmium 

in aqueous samples and found to provide enhanced sensitivity in the analysis 

of these metal species. The overall detection procedure involves preconcentration of 

the heavy metal ions on the electrode surface at open circuit, then cathodic electrolysis 

of the preconcentrated species in an acidic medium followed by a detection 

step using square wave anodic stripping voltammetry (SWASV). The best electrode 

contained 20% (w/w) of powdered picric acid and 80% (w/w) of carbon paste. The 

optimum operating conditions were 2–10 minutes preconcentration period in metal 

ion solution, followed by a 60-second cathodic electrolysis of the preconcentrated 

species and SWASV detection in 0.1 M HCl. Under these conditions the sensitivity, 

limit of detection and linear concentration range of the technique are reported. 

Keywords: heavy metal analysis, picric acid, anodic stripping voltammetry


Posters 

1-P59 Detection of Foreign Organic Substances in 

Water and Biological samples 

E. Savelieva, N. Goncharov, N. Koryagina, N. Khlebnikova, A. Radilov 

Research Institute of Hygiene, Occupational Pathology and Human Ecology, 

Saint-Petersburg, Russia. 

esavelieva @mail.ru 

In spite of rapid development of liquid chromatography and capillary electrophoresis, 

gas chromatography coupled with mass-spectrometry (GCMS) remains to 

take leading positions in the area of analysis of foreign organic substances in water 

and biological tissues. The method of solid-phase microextraction greatly extends 

possibilities of GCMS, taking into account sensitivity and selectivity of analysis, as 

well as unique opportunities to overcoming the matrix effect, that allows to develop 

universal procedures of analysis of toxic compounds in water, beverages, foods, 

environmental objects and biological samples (blood plasma, urea, organ and tissue 

homogenates). The latter is of great importance in practice of environmental 

expertise, toxicological and forensic laboratories. In our report we’ll give examples 

of solving problems as applied to organophosphates and fluoroacetates for direct 

GCMS analysis with their preliminary derivatization both in the sample and on 

microfiber, as well as reactivation products of toxic compounds. 

Keywords: gas chromatography, mass spectrometry, microextraction, 

organophophates, fluoroacetate 

1.


Posters 

1-P60 Determination of Sulfonamides in water 

using Multiwalled Carbon Nanotubes Solid 

Phase Extraction and HPLC with Fluorescence 

Detector 

1. 0 

Stefania Simon, Dan Lupu, Alexandru R. Biris , Constantin Bele 

National Institute for Research & Development of Isotopic and MolecularTechnologies 

R-400293, Cluj-Napoca, Romania 

stefania@itim-cj.ro 

This work focuses on the application of SPE- HPLC analysis of six commonly 

used sulfonamides (sulfadiazine –SDZ, sulfapyridine –SPY , sulfamerazine –SRZ , 

sulfisoxazole –SFX, sulfamethoxazole –SMO and sulfadimethoxine –SDM) in water 

samples.The method involves pre-concentration and clean-up by solid phase extraction 

( SPE ) using multiwalled carbon nanotubes 

( MWCNTs ) as adsorbent. . Parameters that might influence the extraction 

efficiency such as the pH sample and the sample volume were optimized in details. 

Final analysis was carried out by high performance liquid chromatography ( HPLC 

) coupled with fluorescence detection. Recoveries were ranged from 53.6 to 93.5 % 

with relative standard deviation below 7.3%.Limits of quantification were in the 

range of 22–40 ng L –1 . 

Keywords: Multi-walled carbon nanotubes; solid phase extraction; 

sulfonamides; HPLC


Posters 

1-P61 Microbiologial Remediation of Metal- 

Contaminated Soil 

Valéria Snopková, Katarína Jablonovská 

Iinstitute of Geotechnics, Slovak Academy of Sciences 


snopkova@saske.sk 

Heavy metals are widely used in many branches of industry and agriculture 

and hence they appear widespread in the environment. Unlike organic pollutants, 

heavy metals do not undergo degradation; therefore they tend to accumulate in 

soils and sediments. The occurrence of heavy metals in soils and their impact on 

ecosystems depends on several factors including the character of the contaminant, 

nature of the soil, climate and anthropogenic activities. Heavy metals affect the population 

of soil microflora and vegetation; enter the food chain, pose a threat to health 

of animals and humans and cause consequent economical problems. 

Several approaches have been applied for the remediation of soil contaminated 

with heavy metals. Conventional physical and chemical techniques should 

be combined with biological methods in order to meet ecological and economical 

acceptance. Bioremediation technologies use the abilities of living organisms for the 

attenuation of metal contaminations. Processes such as oxidative or reductive metal 

dissolution or precipitation, metal volatilization, sorption or accumulation are commonly 

used. Microorganisms, particularly bacteria and archaea, are able to mediate 

variety of processes that lead to mobilization/immobilization or transformation of 

heavy metals. Presented work is a review of the bioremediation methods for cleaning 

of soil contaminated with heavy metals. 

Keywords: heavy metals, bioremediation 

1. 1


Posters 

1-P62 Distribution of Phthalic Acid Esters (DEPH, 

DBS) in Chicken Tissues and Organs 

1. 2 

Vlasta Stancová, Lenka Krátká, Alžběta Jarošová, Jiří Harazim, Pavel Suchý 

Mendel University of Agriculture and Forestry Brno 

Faculty of Agronomy, Zemedelska 1, 613 00, Brno, Czech Republic 

xwegsch0@node.mendelu.cz 

Phthalates, largely used as plasticiesers for PVC, are among the most important 

industrial chemicals and are produced in great quantities. These substances are 

also used in many other areas, such as in production of medical devices, packaging 

materials, toiletry products, housing equipment and textiles are thus omnipresent 

in the environment. 

Phthalic acid esters (PAEs) are considered highly hazardous to human health 

because they disrupt the hormonal balance and impair reproduction and development. 

PAEs have long been suspected in connection with rising infertility rates, particularly 

among men. Some of them are considered as potential carcinogens, teratogens 

and mutagens. Additionally, phthalates are suspected to trigger asthma and 

dermal diseases in children. 

The most common and toxic PAEs are di-(2-ethylhexyl)phthalate (DEHP) and 

dibutylphthalate (DBP). The aim of this study was to investigate real content of 

these PAEs in samples of chicken’s muscle, skin, fat and liver. Chickens were fed by 

commercial feedstuffs with various content of PAEs. Phthalates were determined 

by HPLC with UV detection. It was confirmed lipolytical character of PAEs. The 

highest amount of phthalates was accumulated in fat (1,36–3,27 mg.kg -1 DEHP; 0,47– 

1,28 mg.kg -1 DBP) and skin (1,10–1,60 mg.kg -1 DEHP; 0,36–0,57 mg.kg -1 ). 

Keywords: phthalates, chicken, feed


Posters 

1-P63 Purification Process Influence on the PAH 

Determination in Real Soil Samples 

M. Stoupalová, M. Vávrová, L. Mravcová, V. Večerek 

University of Veterinary and Pharmaceutical Sciences Brno, 

Faculty of Veterinary Hygiene and Ecology 

Palackého 1–3, 612 42 Brno, Czech Republic 

stoupalovam@vfu.cz 

The study submitted was focused on the comparison of pre-analytical processes 

which can significantly influence results of xenobiotics’ trace and ultra-trace 

analyses and analyses of bio-active substances. A choice of inappropriate isolation 

and purification process can decrease analytes’ yield by up to 80%. Extracts were acquired 

via soil samples’ microwave extraction and subsequently purified by means 

of column chromatography. There were two purification processes compared. During 

the first process, the sorbent used was silica gel and during the second process, 

sorbents used were silica gel and florisil. On the basis of results acquired, it can be 

claimed that both processes mentioned are appropriate for PAH extracts purification. 

However, it is important to draw the attention to the fact that during such 

group determination (18 PAH agents), the structure and chemical properties of 

individual PAH are also substantial. The difference was evident namely at higher 

molecular mass PAH, at which better result were reached via silica gel/florisil purification 

process. 

This work was supported by the grant given by Ministry of Education, Youth 

and Sports of the Czech Republic no. MSM 6215712402. 

Keywords: PAH, clean-up, soil 

1.


Posters 

1-P64 The Optimalisation of SPME Method for 

Determination of Essential Oils in Spice 

1. 

M. Stoupalová, H. Pleskačová, M. Vávrová, L. Mravcová, V. Večerek 

University of Veterinary and Pharmaceutical Sciences Brno, 

Faculty of Veterinary Hygiene and Ecology 

Palackého 1–3, 612 42 Brno, Czech Republic 

stoupalovam@vfu.cz 

The main subject of this contribution was an optimalization of solid phase microextraction 

(SPME) method used for the determination of contentual substances 

in spice. During the SPME method development, there were tested two stationary 

phases of fibres used, as well as the sorption length, preheating and sorption temperature. 

The optimalized method proceeds in the following fashion: sample preheating 

for 5 minutes, 15 minutes’ sorption per fibre PDMS/DVB (65 mm) at the 

temperature of 30 °C from the head-space. Analytes were determined via GC/MS. 

Contentual substances identification was performed on the basis of comparison to 

the mass spectra library. By means of this method, samples of thyme, marjoram, 

origanum and caraway were measured. 

This work was supported by the grant given by Ministry of Education, Youth 

and Sports of the Czech Republic no. MSM 6215712402. 

Keywords: SPME, GC/MS, spice


Posters 

1-P65 DTA and Fluorescence Spectra of Humic Acids as 

Indicators of Human Influence on Soil 

Nora Szombathová, Jörg Luster, Božena Debska, Anton Zaujec, 

Milan Macák, Erika Tobiašová, Juraj Chlpík, Vladimír Šimanský 

Slovak University of Agriculture 

Tr. A. Hlinku 2, 949 76 Nitra, The Slovak Republic 

Nora.Szombathova@uniag.sk 

We investigated differences in absorptive, fluorescence and thermogravimetric 

characteristics between humic acids (HA) isolated from humus horizons of soils 

with similar properties, but located in different ecosystems (agroecosystem and 

nearby forest ecosystem). 

Obtained results showed that values of absorbance ratios were significantly 

lower in HA isolated from cultivated soil compared to forest. This indicates higher 

degree of nucleus condensation and/or higher aromaticity of HA isolated from cultivated 

soil. Fluorescence spectra of the HA exhibited two major signals - one indicated 

highly conjugated aromatic compounds and a second peak indicated simple 

phenolics. Peaks were located at the same positions, and differed only in fluorescence 

intensity, which was higher for HA isolated from cultivated soil. The degree 

of aliphaticity ascertained thermogravimetrically was lower in HA isolated from 

cultivated soil compared with forest soil, what confirmed higher condensation or 

humification degree of HA isolated from cultivated soil. 

The results presented in this work confirmed, that investigated characteristics 

of HA can be a sensitive index of structural changes between cultivated and natural 

soils, thus, they can be used as an indicator of anthropogenic influence on soil. 

Keywords: fluorescence spectra, DTA analysis, humic acids 

1.


Posters 

1-P66 Evaluation of Heavy Metals Mobility in the 

Sediments from the Hnilec River, Slovakia 

1. 

Oľga Šestinová, Ján Brehuv, Jozef Hančuľák ,Tomislav Špaldon, Erika 

Fedorová 

Institute of Geotechnics, Slovak Academy of Sciences, 


sestinova@saske.sk 

The aim of our work was to determine released, potentially mobile and mobile 

heavy metals (Cu, Ni, Pb) forms in the contaminated sediments from the Hnilec 

river (Slovakia). 

The sediment samples from eight localities from Hnilec River situated in the 

area of water reservoir Ruzin, water reservoir Palcmanská Maša were analysed. 

The one-shot extraction of sequential leaching according to Tessier procedure 

was used for isolation of individual forms. The extraction was based on already 

known concentrations of total heavy metals contents, which have been observed for 

many years. 

The bottom sediments occuring in water reservoir are results of erosion and 

sedimentation process in their river-basin. They accumulate different pollutants, 

which have natural or anthropogenic origin. 

Heavy metals in sediments occur in various forms and bonds. Their binding 

forms show various mobility degrees, various forms of transport to subterranean 

and surface waters and bio-facility for plants and animals as well. 

Except the released forms, metals can be fixed also in the potentially mobile 

and mobile forms that are the most important for evaluation of the soil bio-toxicity. 

These metals may come directly from weathering process of rocks, where the 

soil-forming process influences their concentration and distribution. Moreover, they 

also come into sediment as result of the anthropogenic human activity. 

Keywords: sequential extraction, sediment, pollution


Posters 

1-P67 Sulphates Reduction from Waste Water of Old 

Mine Loads 

T. Špaldon, J. Brehuv, J. Hančuľák, E. Fedorová, O. Šestinová 

Institute of geotechnics SAS 

Watsonova 45, 043 53 Košice 

spaldon@saske.sk 

This contribution briefs about sulphates reduction of acid mine water. We used 

physical and chemical methods of cleaning, like precipitation, neutralisation, sorption 

and ion-changing by ion-changers. The first step was testing of sulphates reduction 

by Ca(OH) 2 and Al- ions (sodium aluminate) By this method was achieved almost 

100% effect of sulphates elimination. Next step was testing of various kinds of 

sorbents, ion-changers and then mutual combinations. (active carbon, zeolite from 

N.Hrabovec, annorganic composite sorbent Slovakite, ion-changers katex and anex) 

All these tests were performed in dynamic regime. Conclusion of contribution is 

grafical comparing of sulphates reduction effects by selected reagents. 

Keywords: acid mine water, dynamic regime, sorption, ion-changers, 

precipitation 

1.


Posters 

1-P68 Arsenic Removal From Water by Synthetic 

Akaganeite 

1. 

Katarína Štefušová, Miroslava Václavíková, George P. Gallios, 

Štefan Jakabský 

Institute of Geotechnics, Slovak Academy of Sciences 

Watsonova 45, SK-043 53 Kosice, Slovakia 

vaclavik@saske.sk 

Synthetic akagenite (β-FeOOH) was studied as potential sorbent for the removal 

of arsenic from model aqueous solutions. The material was prepared by hydrolysis 

of partially neutralized FeCl 3 , and fully characterized by chemical analysis as 

well as powder X-ray diffraction, electron microscopy TEM/SEM, specific surface 

measurements (BET). Specific surface area of sorbent was 151.30 m 2 /g. Electrophoretic 

mobility measurements of akaganeite particles and consequently zeta potential 

calculations were performed in order to describe the surface charge of the sorbent. 

The point of zero charge (PZC) of akaganeite was achieved at pH 7.56. Moreover, 

the chemical speciation of water solutions containing arsenic oxyanions in various 

ratios was calculated with the aid of computer program and their interaction with 

Fe oxide species is discussed. 

Arsenic removal from aqueous solutions was investigated using batch adsorption-equilibrium 

experiments. Initial As concentration was in the range 20-200 mg/L 

and the sorbent concentration was 2 g/L. All experiments were carried out at ambient 

temperature (22±1°C) The effect of pH, ionic strength and initial arsenic concentration 

were investigated. Experimental data were modeled using Freundlich 

equation and adsorption parameters (K, b, R 2 ) were determined. Maximum sorption 

capacity of akaganeite was 49.97 mg of As per g of sorbent. 

Keywords: arsenic, akaganeite, sorption, zeta potential


Posters 

1-P69 The EPR Study of Particulate Matter 

Nadežda Števulová, Adriana Eštoková, Pavel Stopka 



Vysokoškolská 4, 042 00 Košice, Slovak Republic 

Nadezda.Stevulova@tuke.sk, 

In recent years the role of particulate matter in environmental and health areas 

has come under increased attention of experts and public. The PM 10 and PM 2,5 concentrations 

show much higher correlation to the negative health effects in comparison 

to the total suspended particulate matter. Building constructions and building 

materials play an important role in indoor particulate matter contamination due to 

particles infiltration through joint construction leakages and material distribution 

and deposition processes. 

The dust particles are dangerous not only because of their high concentrations 

in dependence on their particle size but also because of their nature. The surface of 

the inhaled particles, which determines the biological response, is large. The surface 

reactivity of particles depends on the number of reactive spots on the dust particles 

surface and on the distribution of various sites (free radicals and paramagnetic species) 

at the surface. It determines the pathogenic potential of inhaled particles. 

This work is concerned with the particulate matter reactivity investigation by 

concentration of surface active centres. The determined surface spin concentrations 

obtained from EPR spectra were high. 

The presence of transition metal ions in a low oxidation state, typically iron 

Fe 3+ , copper Cu 2+ and manganese Mn 2+ which have to be regard as a possible cause 

of toxicity were determined. 

Keywords: particulate matter, surface active centers 

1.


Posters 

1-P70 Selection of Packing Materials for Biofilter 

Development 

1. 0 

Iveta Štyriaková, Alexandra Vašková 

Department of Biotechnology, Institute of Geotechnics of the Slovak Academy of Sciences, 

Watsonova 45, 043 53 Košice, Slovakia 

bacil@saske.sk 

Biofiltration is a potential alternate process for treatment of large volumes of 

wastewaters containing low concentration of heavy metals. In order to select a suitable 

packing material for the treatment of polluted water and better understand the 

influence of the packing material properties upon the bacterial cell adhesion and the 

removal efficiency of three metals. 

The individual mineral phases of silicates (zeolite, bentonite, quartz sands) and 

carbonates (magnezite, calcite) can control an efficiency of biofiltration when sorption 

and precipitation of metals occurs. Moreover, in both cases, Zn, Cu, Pb were 

accumulated at the mineral and cell surface as precipitates. 

Removal of Zn from solution by silicate was lower efficient, 20% Zn by zeolite, 

46% by bentonite, 21% by quartz sands was removed from the 0.5mM solution, 

while up to 55% by calcite and 88% by magnezite. Removal of Cu was similar approximately 

24%. Pb was very effective removed 93% by bentonite and magnezite 

and 74% by zeolite and calcite. The affinity series for bacterial removal of these metals 

decrease in the order Pb > Zn > Cu. 

Keywords: sorption, minerals


Posters 

1-P71 Antibiotic Effects Of The Naphtoquinonic 

Derivatives On Gram-Positive And Gram- 

Negative Germs 

Radu Tamaian, Nadia Păun, Andreea Iordache, Raluca Vremeră, 

Ştefania Broscăţean 

National Research and Development Institute for Cryogenics and Isotopic Technologies – ICIT 

Râmnicu Vâlcea 

Uzinei Street No. 4, PO 4, BOX 10, Zip Code 240050, Râmnicu Vâlcea, Romania 

tradu@icsi.ro 

Intestinal bacteria and enterococcus are known as being contaminated pathogenetic 

agents of aliments and water. In this paper it was followed the antibiotic 

effect (bacteriostatical and bactericid) of dichlor derivatives of 1,4 naphtoquinones: 

2,3-dichloro-1,4 naphtoquinones (diclone) on those pathogenetical agents. For 

testing there were used both Gram-positive coccus Enterococcus faecalis (ATCC 29212 

and ATCC 19433), and Gram-negative motile bacteria Enterobacter aerogenes (ATCC 

13048). Diclone‘s grade of remanence, in the culture medium, it was determined 

through chromatographical techniques. 

Keywords: enterobacteria, enterococcus, diclone 

1. 1


Posters 

1-P72 Practical Application in Agriculture of the 

Magnesium Products Industry Waste 

1. 2 

Lidia Taubert, Hortensia Rădulescu, S. A. Kiss, R. Kastori, 

Ecaterina Princz, Éva Stefanovits-Bányai 

Chemistry Institute – Timişoara of the Romanian Academy 

Bv. Mihai Viteazu No.24, 300223, Timişoara, Romania 

lidiat@acad-icht.tm.edu.ro 

The paper presents the impact of some mineral additions resulted as waste 

from the magnesium products industry on soil fertility and oat plants (Avena sativa 

L.) nutrition. 

The industrial process of magnesium carbonate and oxide manufacturing from 

dolomites by carbon dioxide leaching generates large amounts of calcium carbonate 

as waste; this also contains appreciable amounts of magnesium carbonate and 

low contents of solid impurities like iron, copper, manganese, zinc and chromium 

compounds. Crusts deposed on the same industrial equipment could represent a 

second source of mineral supplements for the soil treatment. 

Four different doses of each mineral addition were administered on a low fertile 

acid soil. The effects on soil indicate that its reaction turns from acid to neutral and 

an increase of the essential and trace elements content was established. The results 

show that both mineral supplements have improved the seeds germination as well 

as the plants growth and nutrition, reflected in the number of risen plants and plant 

size. In order to improve other physiological characteristics of oat plants and the 

uptake process of nutritive elements as well as the protein content, an ammonium 

nitrate fertilizer was applied too. 

The mineral supplements from the magnesium products industry were successfully 

tested as amendment for low fertile acid soils and as nutrients for oat 

plants in mixture with a nitrogen fertilizer.


Posters 

1-P73 Possibilities of Objective Odorisation Control 

of Natural Gas 

Daniel Tenkrát, Ondřej Prokeš, František Humhal 

Ústav plynárenství, koksochemie a ochrany ovzduší; VŠCHT Praha 

Technická 5; 166 28 Praha 6 

tenkratd@vscht.cz 

The possibility of use of the objective odorisation control of natural gas instead 

of the subjective method is discussed in this paper. The subjective method of odorisation 

control is dependent on individual olfactory perception of each examiner 

who is responsible for the measurement while the objective method is based on 

instrumental (analytical) estimation of an odorant concentration in natural gas. 

The aim of these experiments was to verify a minimal safe odorant concentration 

in natural gas which would meet the requirements for evoking the warning 

grade of an odorisation level (i.e. at latest at 20% of lower explosion limit in mixture 

with air). 

Keywords: odorisation, natural gas, measurement 

1.


Posters 

1-P74 Contamination of Soil and Alimentary wheat in 

Zemplínska Polluted Area 

1. 

Ján Tomáš, Juraj Čéry, Ladislav Lahučký, Janette Musilová 

Department of Chemistry, 

Slovak Agricultural University in Nitra, Slovak Republic 

jan.tomas@uniag.sk 

Metallic pollution of soil in Zemplínska polluted area has begins by uptake of 

heavy metals into the soil, mainly in vicinity of chemical and industrial factories as 

well as of many others sources. 

For inorganic risky elements are considered all risky elements and their compounds 

of mineral nature, which are in specific concentration and their activity affect 

harmfully on compounds of environment and also on soil. They decrease yield 

production as well as their technological, nutritional and sensorial value, mainly 

health safety and safety of growth agricultural products. Significantly are monitoring 

these risky elements: Cr, Pb, Cd, Zn, Cu, Hg, Ni, Co, Se, As and F. From observed 

of heavy metals the enhanced limit value for pseudo-total content of heavy metals in 

aqua regia were evaluated for Cd, Ni and Co. For Cd the limit value is 0.7 mg.kg -1 , 

it was exceed at five gained points in range 0.70–0.96 mg.kg -1 . Interval of Cd content 

was in range 0.40–0.96 mg.kg -1 . The highest enhanced limit value was 37 %. Contamination 

of soil was in one case obvious also for Ni and enhanced limit value was 

15.6 %. The most significant enhanced limit value by Co was 15.0 mg.kg -1 ; this value 

was exceeded at seven gained positions. The highest of overloading was 46.6 %. 

Keywords: heavy metals, risky elements, soil, gained points


Posters 

1-P75 Determination of Changes in Soil Organic 

Matter Content through Carbon and Nitrogen 

Labile Fractions 

Tomáš Tóth, Erika Tobiašová, Vladimír Šimanský 

Department of Pedology and Geology, 

Slovak Agricultural University in Nitra 

Erika.Tobiasova@uniag.sk 

We studied the suitability of carbon and nitrogen labile fractions for determination 

of soil organic matter (SOM) changes in farming systems. Research focus on 

possibilities of soil organic matter changes evaluation through C T -total organic carbon, 

C L -labile carbon, C NL -non-labile carbon, L C -lability of soil organic carbon, LI C 

-lability index of carbon, CPI -carbon pool index, CMI -carbon management index, 

N T -total nitrogen, N L -potentially mineralizable nitrogen, N NL –non-labile nitrogen, 

L N -lability of soil nitrogen, LI N -lability index of nitrogen, NPI -nitrogen pool index 

and NMI -nitrogen management index. In 1990, the field experiment was established 

on loamy Orthic Luvisol. Content of labile carbon (C L ) was in average higher 

in ecological farming system (ES) 2175 g.kg -1 than in integrated farming system (IS) 

1854 g.kg -1 . Values of C L were higher in fertilized variants 2206 g.kg -1 than in variants 

without application of fertilizers 1823 g.kg -1 . These parameters are more sensitive 

than total carbon and nitrogen contents for determination of changes in SOM in 

shorter period. They seem to be suitable for agro-ecosystems, as well. From tested 

indexes, L C and CMI were the most sensitive on SOM changes determination. 

Keywords: Labile Carbon and Nitrogen; Soil Organic Matter 

1.


Posters 

1-P76 Distribution of Heavy Metals in Soil 

1. 

Tomáš Tóth, Alena Vollmannová, Tomáš Ján, Peter Lazor 

Department of Chemistry, Slovak Agricultural University in Nitra 

E-mail: tomas.toth@uniag.sk 

The soil quality is derived from its loading by hazardous substances. The loading 

of soil occurs when the soil is not able to lower the negative influences of the 

risk heavy metals. The reference value, which represents natural content of heavy 

metals in soil, forms the basis for evaluation of the content of heavy metals in soil. 

The important parameters for the input of heavy metals from soil into plants are: the 

soil reaction, the content and the quality of organic matter, the nutrition of plants, 

cation exchange and sorptive capacity, the microbiological activity, the oxidation 

and reduction potential, the amount and the quality of the clay fraction of soil and 

the methods of soil cultivation, etc. The extracfability for individual ectractans is 

evident : 2 mol.dm -3 HNO 3 : Cu > Cd > Pb > Zn > Cr; 0,05 mol.dm -3 EDTA : Cu > 

Cd > Pb > Zb > Cr; 0,01 mol.dm -3 CaCl 2 : Cd > Cu > Pb > Zn > Cr. 2. It is necessary 

to emphasize the differences in solubility between the highly contaminated eutric 

cambisols of Stredný Spiš and other analyzed soils. The different solubility is in all 

extractants and heavy metals except the solubility of chromium in 0,05 M EDTA 

and 0,01 M CaCl 2 . It is probably connected with the high portion of heavy metals of 

immissional origin. The other analyzed soil subtypes have a low solubility of Zn in 

HNO 3 and EDTA in luvic stagnosols. 

Keywords: heavy metals, soi hygiene


Posters 

1-P77 Separation and Preconcentration of Arsenic, 

Antimony, Selenium and Tellurium on Modified 

Silicagels for their Determination by ICP-AES 

Kristýna Urbánková, Lumír Sommer, Martin Moos 

Brno University of Technology, Chemistry and Technology of Environmental Protection 


urbankova@fch.vutbr.cz 

Modified silica sorbents SeparonTM SGX C18, C8, CN, NH , RPS and Phenyl 

2 

were tested for preconcentration of 0,25–5 mg.l –1 from 50–1000 ml sample volume 

of As, Sb, Se and Te in the form of ion associates with cationic surfactants. Carbethoxy-pentadecyltrimethylammonium 

bromide (Septonex ® ) in concentration 

0,005 mol.l-1 was suitable for the purpose. Septonex ® with selected organic reagents 

4-(2-Pyridylazo)resorcinol, Pyrollidincarbodithionate, diethyl-dithiokarbamidate, 

thiourea, 8-hydroxyquinoline-5-sulphononic acid and 1,2-dihydroxybenzene were 

successfully used for quantitative sorption at pH 7 and after elution of 10 ml of acetone 

with ethanol in ratio 1 : 1 in the presence of 0,1M HCl. The enrichment factor 

for the elements on Separon ® reaches 100. 

The influence of Na + , K + , Ca2+ , Mg2+ , Fe3+ , Al3+ – – – 2– 3– or NO , Cl , Br , SO4 and PO4 as 

3 

well as the mixture of heavy metal ions on the sorption was studied. Organic solvents 

and the excess of acids were removed by evaporaton prior to the determination 

by ICP-AES. Optimalized procedures were used for the determination of arsenic, 

antimony, selenium and tellurium in the form of anionic speciations in natural 

waters. 

Keywords: ICP-AES, preconcentration 

1.


Posters 

1-P78 Multicomponent Microdetermination of 

Arsenic, Antimony, Tellurium, Selenium Besides 

of Thallium by ICP-MS in Waters 

1. 

Kristýna Urbánková, Jiří Machát, Lumír Sommer 

Brno University of Technology, Chemistry and Technology of Environmental Protection 


urbankova@fch.vutbr.cz 

Arsenic, antimony, selenium, tellurium and thallium in the form of isotopes 

75 As, 82 Se, 125 Te, 121 Sb and 205 Tl were determined for less than 5 µg.L -1 in solution with 

0.5% HNO 3 .The practical detection limits for various kinds of waters are 0.05-0.08 

for µg L -1 75 As, 0.04- 0.03 µg.L -1 82 Se, 0.03-0.01 µg.L -1 121 Sb, 0.3-0.02 µg.L -1 125 Te and 

0.005-0.2 µg.L -1 205 Tl. For 100 µg .L -1 of the element the error does not exceed 20% in 

the presence of 50 mg.mL -1 Ca, Mg, Al, Fe(III) or 10% in the presence of 200 mg.L -1 

Na or K. For multicomponent solutions with 1 – 100 mg.L -1 of Al, Ba, Ca, Cd, Co, Cr, 

Cu, Fe, Mg, Mn, Na, Ni, Pb, Ti, and Zn the signal decrease of the microelements is 

not larger than 35% for 82 Se and 125 Te and 30% for 75 As, 121 Sb and 205 Tl. Internal standards 

such as 72 Ge and 209 Bi can be successfully used with respect to the particular 

mass of the microelements. 

Drinking, surface and mineral waters can be analyzed with spikes ≤ 1-5 µg.L -1 

75 As, 82 Se, 121 Sb, 125 Te and 205 Tl, sea and mine waters using standard addition method 

when the signals were always previously corrected against ultra pure water. 

Keywords: ICP-MS, water


Posters 

1-P79 Treating Wastewater by Using of Bioceamic 

Filters 

A. Vaskova, I. Styriakova, D. Kupka, V. Snopkova 

Department of Biotechnology, Insitute of Geotechnics of the Slovac Academy of Sciences 

Watsonova 45, 043 53 Kosice, Slovakia 

avaskova@saske.sk 

The using of bioceramic filters based on iron oxides, clay minerals and bacteria 

could be an alternative way to remove heavy metals from industrial effluents. 

Synthetic iron oxides including goethite, lepidocrocite, maghemite, magnetite 

and hematite were prepared in the laboratory by hydrolysis of Fe 3+ acid solutions or 

by controlled oxidation of Fe 2+ solutions followed by Fe 3+ precipitation. The main 

mineral phases were confirmed by X-ray diffraction and Mössbauer spectroscopy. 

A number of studies on metal uptake using clay minerals have been conducted 

and results have shown good adsorption properties. Due to its metal-binding capacity, 

high surface area, natural materials such as zeolite, bentonite, kaolinite will be 

used for the treatment of metal-contaminated waste waters. 

In this study two bacterial strains were isolated from the heavy metal pollluted 

waste water of industry plant: Bacillus megaterium and Pseudomonas diminuta. 

The resistance of bacterial strains to copper, zinc and chromium was tested. The 

bacterial isolate Bacillus megaterium could grow at a concentration ranging from 

52–780 mg Cr/l , 52–260 mg Zn /l and max 52 mg Cu /l and Pseudomonas diminuta 

at a concentration ranging from 52–520 mg Cu/l , 52–260 mg Zn /l and max 52 mg 

Cr/l , at temperature 25°C. 

Keywords: bioceramic filter, bacteria 

1.


Posters 

1-P80 Selected MUSK Compounds in Abiotic and Biotic 

Samples 

1.100 

M. Vávrová, D. Štenclová, E. Blahová, J. Mareš, M. Mášová, J. Čáslavský 




Synthetic musk compounds are fragrance substances commonly used as fragrant 

constituents of detergents, cleaning and washing agents, and cosmetics. At 

present, there are more than three thousand synthetic fragrances being used for 

perfuming commercial products. However, some musk compounds have proved to 

accumulate in the environment as well as in mammal tissues of human body, and 

hence classified as persistent organic pollutants (POPs). This thesis was focused on 

some synthetic fragrances produced by the AROMA Prague Co. and musk xylene, 

musk ketone and tonalide. The aim of this study was to perform a method optimisation 

for the determination of selected fragrances in waste water, sewage sludge and 

tissues of fish. Different extraction techniques for the separation of studied compounds 

from solid matrices were compared. The identification and quantification of 

analytes was carried out by high resolution gas chromatography – mass spectrometry 

(HRGC/MS). 

Acknowledgement: Financial support from the COST Action 636, Project 

No. OC-183 

Keywords: synthetic musk compounds, waste water, sewage sludge, 

tissues of fish, gaschromatography, mass spectrometry


Posters 

1-P81 Determination of Surfactants Included in 

Sewage Water 

Milada Vávrová, Lenka Langová, Helena Zlámalová Gargošová, 

Kristýna Kubíčková, Vladimír Večerek 




Tensides rank among the surfactants with the ability to reduce surface tension. 

This ability is used to remove impurities. The thesis concentrates on the determination 

of tensides in sewage waters. These substances reach sewage waters from various 

cleansing and washing agents which are used both in households and industry. 

The theoretic part includes the basic classification of tensides, their characteristics 

and the requirements of the legislative of the Czech Republic on their content in 

sewage waters. The thesis is closed with estimation of the results of tenside content 

measuring done on the inflow and outflow of sewage waters on the premises of the 

Veterinary and Pharmaceutical University at Brno. The method of mobile analysis 

based on the reaction between the monitored substance and chemical agent was 

chosen for the tenside measuring. This method results in the origin of a colorful 

compound suitable for spectrophotometric determination. 

Acknowledgement: Financial support from Ministry of Education, Youth and 

Sports under MSM 6215712402 is greatly appreciated 

Keywords: tensides, mobile analytics, arbitration methods, sewage waters 

1.101


Posters 

1-P82 Examination of the Mutual Interaction of Heavy 

Metals in Course of Adsorption from Model 

Solutions 

1.102 

Ján Vereš, Tomáš Bakalár, Milan Búgel, Martin Sisol 


Watsonova 45, SK-043 53 Kosice, Slovakia 

veres@saske.sk 

The research was focused on study of heavy metals removal from aqueous solutions 

using by different sorbents natural zeolite and synthetic zeolite Slovakit. 

The metals of interest were zinc, copper, nickel and lead. Heavy metals removal was 

investigated in batch adsorption-equilibrium experiments. The inicial concentration 

of heavy metals in stock solutions was in the range at 5-1000 mg/L. Sorption experiments 

were carried out at pH 5.5, at the sorbent concentration of 2g/L. All adsorption 

experiments were carried out at ambient temperature (22±1°C). The quantity of 

elements in solution has been determined both before the introduction of sorbent 

and after the equilibrium time of 24 hours. The experimental data were modeled 

with Langmuir, Freundlich and Redlich-Peterson isotherms. Best sorption capacity 

was obtained on synthetic Slovakit, decrease in this order Pb>Ni>Cu>Zn. 

Keywords: adsorption, heavy metals, adsorption isotherm, zeolite, Slovakit


Posters 

1-P83 Fungicidal Effect of Printed Titanium Dioxide 

Layers 

Mária Veselá, Michal Veselý, Petr Dzik, 

Jana Chomoucká, Lenka Šupinová 



vesela@fch.vutbr.cz 

Microorganisms form an essential part of Earth life. They range almost everywhere. 

In course of many years people learned to use microorganisms to their benefit. 

But in many cases the microorganisms are unwanted and detrimental. There 

is a heavy risk of microbial contamination especially on places which require very 

high cleanness. This is the reason for research and development of new disinfection 

methods. In the last years the so-called self-cleaning surfaces have been of a great 

interest. Their self-cleaning properties are based on the photocatalytic activity of 

titanium dioxide thin layers. 

UV radiation is required for generating of reactive oxygen radicals on titanium 

dioxide surfaces. Thus, such surfaces work as fungicidal or bactericidal agent. 

In this work, the fungicidal effect of titanium dioxide was studied. Titanium 

dioxide layers were prepared by sol-gel method and coated on soda-lime glasses by 

an inkjet printer. Candida vini phocatalytic degradation was studied in dependence 

of surface structure of printed titanium dioxide layers. Cell viability was measured 

using epi-fluorescence microscopy and kinetics of this photokilling process was 

evaluated. 

Keywords: yeast, printed layers, titanium dioxide 

1.10


Posters 

1-P84 Photocatalytic Disinfection of Water Using Ag/ 

TiO 2 

1.10 

Mária Veselá, Michal Veselý, Jana Chomoucká, Michaela Lipenská 



vesela@fch.vutbr.cz 

Common disinfection methods such as chlorination, ozonation and UV irradiation 

have their limitations. In the case of chlorination and ozonation, trihalomethanes 

and other carcinogenic disinfection by-products maybe generated. As for UV 

disinfection, the process may not be effective enough as some of bacteria may have 

mutated and developed resistance towards UV light 

New methods which can be capable to replace chlorination and ozonation in 

water treatment are of great interest. A photocatalytic process is one of them. By 

means of UV radiation, very reactive oxygen radical on titanium dioxide surfaces 

are generated. These radicals are very strong oxidative agent with very high disinfection 

potential. 

The photocatalytic disinfection process of yeast Hansenula anomala in water was 

studied in a multi tube flow reactor. The inner walls of tubes were coated by titanium 

dioxide using sol-gel process and dip-coating method. The kinetics of photocatalytic 

disinfection on pure titanium dioxide and titanium dioxide with deposited 

metallic silver islands were evaluated. 

Keywords: photokilling, yeast, flow-reactor


Posters 

1-P85 Laboratory Study of Arsenic Mobility in Stream 

Sediments and Impoundment Material using 

Column Experiments 

Veronika Veselská, Edgar Hiller 

Comenius University in Bratislava, Faculty of Natural Sciences, Department of Geochemistry 

Mlynská dolina, 842 15 Bratislava, Slovak Republic 

veselska@fns.uniba.sk 

High arsenic contents in the impoundment located near the village of Poša in 

eastern Slovakia, represent a serious environmental problem because of As mobilization 

from the impoundment material, its release in contaminated stream sediments 

of the Kyjov brook and its behaviour in contaminated stream sediments and 

impoundment material using column laboratory experiments was studied. 

The results of the column experiments showed that As release from the solid 

samples by water was likely controlled by Fe and Mn oxohydroxides and pH of 

the sediments and leachates. It was also observed that extraction time was an important 

factor influencing the As release. The extent of As contamination in stream 

sediments and impoundment material was 20- up to 401- times higher as compared 

with the site-specific As background concentrations. 

The leaching behaviour of As was investigated in three stream sediment samples 

of the Kyjov brook (KY-100, KY-1000 a KY-2000) and one sample of the impoundment 

material (KY-0). The relative mobility of As was inversely proportional 

to the oxalate-extractable Mn content (R = -0,960*, α


Posters 

1-P86 Antibiotics in the Environment 

1.10 

H. Vítečková, L. Vydrová, M. Vávrová, L. Mravcová 


Purkyňova 118, Brno 612 00 

viteckova@fch.vubr.cz 

Antibiotics used in human and veterinary medicines are important group of 

drugs. Using of these compounds leads to their spreading and acccumulating into 

the environment where undesirable resistance is formed. It has been proved that 

they block biological processes in wastewater treatment plants. They also harm natural 

ecosystems and may concentrate in food chain. 

Tetracyclines and sulfonamides were determinated in this work. The samples 

were collected from the municipal wastewater treatment plant and the veterinary 

wastewater treatment plant in Brno, The Czech Republic. The simple extraction 

method for sulfonamides and tetracyclines in wastewater has been developed. Sulfonamides 

were extracted by liquid-liquid extraction with ethylacetate and evaporated 

by a rotary vacuum evaporator. The evaporation residues were dissolved in 

methanole and mobile phase. The mixture of acetonitrile and formic acid in purified 

water was used as the mobile phase. Tetracyclines were extracted by SPE and acetonitrile 

and sulfuric acid was used as the mobile phase. The samples were filtered 

into the amber glass vials by membrane filters. The HPLC instrument equipped 

with diode array detector was used for analysis of the samples. 

Finantial support from the COST, Action 636, Project No. OC-183 is greatly 

acknowledged. 

Keywords: tetracyclines, sulfonamides, HPLC


Posters 

1-P87 The Fluorimetric Determination of Aluminium, 

Gallium and Indium with 8-Hydroxyquinoline- 

5-sulphonic acid in Aqueous and Submicellar 

Medium 

Šimon Vojta, Lumír Sommer 

Institute of Environmental Protection, Faculty of Chemistry, Brno University of Technology, 

Purkyňova 118, 612 00 Brno (Czech Republic) 

vojta@fch.vutbr.cz 

Collinear fluorescence spectra appear for complexes with 8-Hydroxyquinoline- 

5-sulphonic acid (QSA) with λ max 495 nm for Al, 504 nm for Ga and In in dependence 

on pH. The fluorescence is highly increased in the presence of submicellar 

concentration of cationic surfactants, especially Zephyramine. The stoichiometry of 

the components in the complexes is M:L = 1:1 – 1:3 in the absence of surfactant and 

M:L = 1:3 in the presence of cationic surfactant. The optimal concentration range for 

the determination of Al, Ga and In is 0.03-1 g.L -1 , the detection limits 0.03-0.04 g.L -1 

in the presence or absence of surfactant for normal and first derivative spectra. Fe 3+ , 

fluoride, phosphate and excess of nitrate and bromide interfere in the absence and 

presence of Zephyramine. 

The first derivative spectra for pH 3, 8 and 4 with λ max 460 nm for Al, 472 nm for 

Ga and 478 nm for In do not bring advantages. 

For the determination of Al, Ga, and In pH 4, 3, and 8 in the presence of hexamethylentetramine 

or tetraborate and Zephyramine is suitable. 

Keywords: 8-hydroxyquinoline-5-sulphonic acid, Aluminium, Gallium, Indium 

complexes, fluorescence, cationic surfactants, first derivative spectra 

1.10


Posters 

1-P88 Surgical Polyester Fabric 

Impregnated by Cross-Linked Collagen 

1.10 

Lucy Vojtová,Pavel Filka, Josef Jančář 

Institute of Materials Science, Faculty of Chemistry, Brno University of Technology, 

Purkyňova 118, 612 00, Brno, Czech Republic 

xcfilka@fch.vutbr.cz 

Proposed work was focused on the impregnation of biomedical permanently 

implantable polyester (PES) fabric with collagen type I in order to improve the biocompatibility 

of surgical fabric used for long time fixation of different organs. 

Coated air-dried collagen was subsequently cross-linked either by a commonly 

used formaldehyde resins or by a new water-soluble nontoxic carbodiimide solution. 

The cross-linked collagens embodied very good adhesion to the implantable 

fabric from which the collagen did not separate up to the time of total degradation. 

Based on the measured values the best result attained collagen cross-linked by 

carbodiimide, which had the lowest swelling degree related to the highest density 

of polymer network. This cross-linked collagen beard up against the hydrolytic degradation 

up to the 78 days in physiological solution at 37 °C. Within this time the 

cross-linked collagen is able to increase the biocompatibility of commercially used 

implantable fabric. 

Acknowledgement: This work was supported by the Ministry of Education, 

Youth and Physical Training of the Czech Republic under the research project MSM 

0021630501. 

Keywords: Collagen, surgical fabric, cross-linking.


Posters 

1-P89 Soil Hygiene in Old Environmental Burden Areas 

Alena Vollmannová, Ján Tomáš, Daniel Bajčan, Peter Kováčik 

Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra 

Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic 

Alena.Vollmannova@uniag.sk 

The necessity of old environmental burden areas remedy is one of present environmental 

problems. The negative effect of the anthropogenic breach into the environment 

arised in the past still exists. In our work the soil hygiene of three various 

old environmental burden areas of the Slovakia is evaluated. 

The alluvial soils near of Štiavnica river running through the area long-time 

burden by intensive mine activity oriented on polymetallic ore mining are contaminated 

by high Zn (512 –2760 mg.kg -1 ), Cu (100–264 mg.kg -1 ), Cd (3.56–21.2 mg.kg -1 ) 

and extremely high Pb (267–3260 mg.kg -1 ) contents. 

The observed soils surround of previous Nickel Smeltery Sereď can be evaluated 

from the aspect of risk metal contents as “clean” despite of weakly enhanced Cd, 

Cu, Ni and Co soil contents (respectively up to 2.00; 69.6; 49.2 and 24.4 mg.kg -1 ). 

In observed area surround previous Iron Ore Mine in Rudňany in the region 

of Middle Spiš the extremely high soil content of Hg was determined (up to 39.10 

mg.kg -1 ) and the soil contamination by As and Cu was confirmed (up to 43.4 resp. 

114.65 mg.kg -1 ). 

The rezidual metallic soil burden and potential follow contamination of the 

food chain in observed areas presents the potential risk for the human health. 

Keywords: soil hygiene, environmental burden areas 

1.10


Posters 

1-P90 EPR Study on Photoinduced Processes 

of Novel Quinolone Derivatives 

1.110 

Zuzana Vrecková, Vlasta Brezová, Maroš Bella, Viktor Milata 

Institute of Physical Chemistry and Chemical Physics, 

Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava 

Radlinského 9, SK-812 37 Bratislava, Slovak Republic 

zuzana.vreckova@stuba.sk 

Novel quinolone derivatives were synthesized as potential anticancer and antimicrobial 

agents. The presence of extended π-electron system of quinolones results 

in their photosensitive properties; therefore UVA irradiation of quinolones can in- 

duce the photosensitive reactions with phototoxic responses. The photogenerated 

free radicals in the biological systems induce negative processes resulting in cell 

damage. On the contrary, the reactive oxygen intermediates can be used for therapeutic 

purposes in phototherapy or photochemotherapy. 

EPR spectroscopy was applied for the detection of photoinduced generation of 

radical intermediates (especially superoxide anion radical) and singlet oxygen. The 

study of synthesized quinolones photoactivity upon UVA irradiation (metal-halide 

medium-pressure mercury lamp) evidenced that the photoexcitation in the pres- 

•– 1 ence of molecular oxygen resulted in the formation of O and O2 via Type I and 

2 

II photooxidation processes, which accomplished simultaneously. The highest capability 

to produce free radicals and 1O upon irradiation showed novel selenadia- 

2 

zolo-quinolones. The results of EPR investigations served as a basis to select suitable 

quinolone derivatives for the photobiological experiments on the different cell lines, 

as well as a proposal of synthesis of novel quinolone derivatives with the required 

photochemical properties. 

Keywords: quinolones, EPR spectroscopy, UV/vis spectroscopy

1-P91 The Boron in Kraft Pulp Mill 

and Influence In Waste Water 

Milan Vrška, Eva Gemzicka, 


Posters 

STU Bratislava, FChPT, Ústav polymérnych materiálov, odd. chem. tech. dreva, celulózy a 

papiera 

Radlinského 9, 812 37 Bratislava 

eva.gemzicka@yahoo.com 

New chemicals are added to recovery line for process improvement. One of the 

others is Borax (NaBO 3 ) a chemical compound of boron. Borax is accumulated in 

chemical recovery line, but part of it is proceed to waste water. 

Boron and borate were controlled in waste water and sludges. The amount of 

boron in waste water does not depend on pH. The boron is bound to borate and 

other compounds, more of it form small particulates. These particulates cause some 

problems in waste water analysis, therefore filtration is necessary 

Impact of borax addition and conditions in technological process have apparent 

effect of sludges filtration and next analysis. Using the OES ICP analytical method 

we obtained a new knowledge of boron behavior and his impact on technology 

parameters /chemical adding, insoluble matters … / in kraft pulp mill. 

Keywords: borax, boron, borate, recovery line, waste water, elements 

1.111


Posters 

1-P92 Contents of Different Fractions of Sulphur 

in Slovakia Soils 

1.112 

Anton Zaujec, Lýdia Jedlovská, Melánia Feszterová, 

Department of Environmentalism and Zoology, Faculty of Agrobiology and Food Resources, 

Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia e-mail: Lydia. 

Jedlovska@uniag.sk 

In the last years we studied contents of selected fractions of sulphur in Slovakia 

soils: chloride soluble sulphure (ClSS), sulphate sulphure (SS) and heat soluble sulphure 

(HSS). The results we obtained from 41 location, from 52 soil´s profiles: Haplic 

Chernozems (CH), Phaeozems (PH), Haplic Luvisols (LV), Eutric Cambisols (Cm), 

Distric Planosols (PL). Sulphur content these sulphur frations we determined in different 

layers of soil profiles. The results showed, that the highest amount of fractions 

of sulphur was in top layer (0,0–0,2 m). The highest content chloride soluble sulphure 

and heat soluble sulphure was in Phaeozems (ClSS = 947.8 mg kg –1 ; HSS = 433.1 

mg kg –1 ), sulphate sulphure was in Haplic Chernozems (SS = 232.3 mg kg –1 ) in top 

layer of monitored soils. Big differences between accounted values were in the same 

soil profiles in different seasons e.g. Žitavský luh (HSS (spring) = 36.9 mg kg –1 HSS 

(autumn) = 183.74 mg kg –1 ). 

Decresing content chloride soluble sulphure was in monitoring soil’s types following: 

PH < LV < CH < CM < PL, the decresing content sulphate sulphure was CH 

< PH < LV < CM < PL and the decresing content heat soluble sulphure was PH < CM 

< CM < CH < PL. Obtained results gave us the possibilities to evaluate of different 

fractions of sulphur in soils.


Posters 

1-P93 Factors Influencing the Sorption Behaviour of 

Herbicide Acetochlor in Soils and Sediments 

Lenka Zemanová, Edgar Hiller, Zoltán Krascsenits 

Comenius University in Bratislava, Faculty of Natural Sciences, 

Department of Geochemistry 

Mlynská dolina, 842 15 Bratislava, Slovak Republic 

zemanoval@fns.uniba.sk 

Sorption and desorption are believed to be the main processes controlling the 

movement and persistence of organic pollutants in soils. Laboratory study was 

conducted to determine the sorption-desorption behaviour of acetochlor, herbicide 

commonly used in Slovak agriculture. The sorption and desorption were investigated 

using the samples of 8 surface soils, 1 subsurface soil, 3 bottom sediments and 2 

river sediments. According to the results of simple regression analysis, organic carbon 

content of the sorbents was found to be the most important factor influencing 

the extent of sorption processes. Organic carbon content of the soils and sediments 

was significantly correlated to K D coefficient (r = 0.774; P < 0.01). Similarly, desorption 

of acetochlor was inversely proportional to organic carbon content of the soils 

and sediments used (r = -0.81; P < 0.001). Significant relationship found between 

K OC and humic/fulvic acid ratios of the sorbents (r = 0.578; P < 0.05) implies, that 

the organic matter character and properties may influence the sorption affinity of 

acetochlor to organic components of the sorbent. This finding might partly explain 

relatively high variability of K OC values of acetochlor determined in this study (K OC 

= 86–399 l.kg -1 ) and reported elsewhere, too. 

Keywords: acetochlor, sorption 

1.11


Posters 

1-P94 Ecotoxicological Evaluation of the Sludges 

From Waste Water Treatment Plants 

1.11 

Helena Zlámalová Gargošová, Lucie Hellingerová, 

Milada Vávrová 

Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, 



zlamalova@fch.vutbr.cz 

Sewage sludge from waste water treatment plants can be used as fertilizer only 

in case if they don’t show any ecotoxicity. Sewage sludge from different waste water 

treatment plants was evaluated by selected tests of ecotoxicity with respect to their 

intended use. 

For this purpose two alternative toxicity tests, Thamnotoxkit FTM on sense organisms 

Thamnocephalus platyurus and Daphnotoxkit FTM magna on sense organisms 

Daphnia magna were used, and also two phytotests were employed: Sinapis 

alba root growth inhibition toxicity test and Allium cepa L root growth inhibition 

toxicity test. The values of 24hLC50 and 48hLC50 obtained for Thamnocephalus platyurus 

and Daphnia magna testing organisms and 72hIC50 values gained for Sinapis 

alba and Allium cepa testing plants are the basic data for the ecotoxicological assessment 

of the sewage sludges and for classification following the Czech legislation. 

Keywords: sewage sludges, ecotoxicity tests


Posters 

1-P95 Microwave Desulphurization Of Coal 

Ingrid Znamenáčová, Michal Lovás, Silvia Čuvanová, 

Štefan Jakabský 

Institute of Geotechnics, Slovac Academy of Sciences, 

Watsonova 45, 043 53 Košice, SR 

znamenackova@saske.sk 

An application of microwave energy for sulphur removing from the coal prior 

to coal burning is the subject of presented article. Accessibility of coal to desulphurization 

by magnetic separation followed by microwave heating was investigated. 

The coal was subjected to magnetic separation followed by treatment in a microwave 

oven. Pyritic sulphur content was reduced to 40–45 %. 

Desulphurization by the microwaves is closely related to the form of sulphur 

compound in coal, its chemical structure, as well as the chemical activity of the 

leaching. Pulverized samples of coal were desulphurized by the treatment with 

fluid NaOH. 

The microwave heating in classical and microwave oven was realised. The 

caustic-coal mixture was heated by the microwave irradiation in N 2 atmosphere. A 

considerable success was achieved in tests carried out on coal pretreated by heating 

with NaOH in the microwave oven. Reduction in total sulphur content was within 

25 % and 56 %. The decrease of sulphur in coal from Nováky and Cígel was 52–56 %, 

from Donbas 35 %, from Handlová 28 %, and from Sokolov 25 %. It can be stated 

that the microwave heating pretreatment of the feed coal with sodium hydroxide 

solution was successful. It was determined a shortening of smelting reaction time 

at coal desulphurization process carrying out in the mixture with NaOH under application 

of microwave radiation in comparison with the classical technique of desulphurization 

in muffle oven. 

Keywords: coal, desulphurization, microwave, magnetic 

1.11


Posters 

1-P96 The Biological Activity of Tetracyclic Diterpene 

Isolated from The Slovak Brown Coal 

1.11 

Anton Zubrik, Andrea Lauková, Alena Gábelová, Zuzana Valovičová, 

Josef Cvačka, Ľudmila Turčániová 


Watsonova 45, SK-04353 Košice, Slovak Republic 

zubant@saske.sk 

This work deals with extraction, identification and isolation of biologically 

active compound from the Slovak brown coal. Obtained coal extracts were separated 

by column chromatography and TLC to several aliphatic fractions. Isolation 

and structural elucidation of tetracyclic diterpene – 16α(H)-phyllocladane was 

performed. The structure of isolated tetracyclic hydrocarbon was specified using 

GC/MS, NMR, HR-MS. Biological activity of 16α(H)-phyllocladane was evaluated 

in vitro using the human hepatoma cell line HepG2 and the bacterial strains. Two 

endpoints, cell viability and DNA breakage was assessed in phyllocladane-exposed 

human cells. Based on the preliminary results, tetracyclic diterpene manifests only 

weak cytotoxicity in HepG2 cells. Only ~20% reduction of cell viability was detected 

at the highest concentration of phyllocladane (0.5 mg/ml). A mild but statistically 

significant increase of DNA damage was found in phyllocladane-exposed HepG2 

cells compared with negative control (p

Section 2: 

Food Chemistry & Biotechnology 

2.1

2.2

Lectures 

2.


Lectures 

2-L01 Nonsaccharomyces Yeast in Grape Must – 

Advantege or Spoilage? 

Emília Breierová, Jaroslava Kaňuchová Pátková, Ingrid Vajciková, 

Institute of Chemistry, SAS, 

Dúbravská cesta 9, 845 38 Bratislava, Slovakia 

chememi@savba.sk 

The fresh grape must consists of spontaneous microflora from 90- 99 % about 

yeasts. The most important genus is without doubt Saccharomyces cerevisiae which is 

responsible for successive fermentation and good wine quality. Recently the contribution 

of nonsaccharomyces yeast has been widely discussed as there is not definitive 

opinion on their contribution to the wine quality, especially aroma. The genus 

of Rhodotorula, Sporobolomyces, Pichia, Hansenula, Bretanomyces were evaluated from 

the viewpoint of their contribution to the wine aroma. The yeasts separately fermented 

the grape must and the final product was evaluated sensorialy and analytically. 

Some of them - Hansenula and Pichia were considered to act positively on the 

aroma quality, increasing the fruit and/or coconut aroma by higher production of 

sabinyl and isoamylacetate, resp. γ-nonalactone. Some of the apiculate microflora 

– Rhodotorula and Sporobolomyces were evaluated negatively with increased production 

of 3,4-hexanedione, hexanal, eicosene causing the buttery and waxy odour. 

Keywords: wine, aroma, yeast, apiculate microflora 

0 

2.

Section 2 – Food Chemistry & Biotechnology 

Lectures 

2-L02 Approaches to Minimization of Acrylamide 

Level in Foods 

2. 

Zuzana Ciesarová 

VÚP Food Research Institute 

Priemyselná 4, 824 75 Bratislava, Slovak Republic 

ciesarova@vup.sk 

High-temperature processing of foods results in the formation of acrylamide, 

a substance which is associated with the increased incidence of breast, ovarian, and 

endometrial cancer. The presence of acrylamide is widespread in many staple foods 

because it arises from natural occurred compounds during the reaction of amino 

acid asparagine with reducing sugars (glucose, fructose) at temperature higher than 

120°C in the common and desirable process of Maillard reaction. Due to an insistent 

appeal of JECFA (Joint WHO/FAO Expert Committee on Food Additives) since 

2007 for mitigation of acrylamide to the level as lowest as possible addressed to the 

European authorities and institutions, there is a great challenge to find the effective 

ways of acrylamide reduction. 

The minimization concept of acrylamide encompasses several approaches: 1) 

an avoidance of acrylamide formation by a decline of its precursors in raw material; 

2) a modification of heat treatment conditions; 3) an enhancement of acrylamide 

removal. The ways which were examined at VUP Food Research Institute in Bratislava 

would be overviewed. 

Acknowledgement: This work was supported by the Slovak Research and 

Development Agency under the contract No. COST-0015-06. 

Keywords: acrylamide, minimization


Lectures 

2L-03 Solid State Fermentation as a Tool for 

Preparation of Bioproducts Enriched with 

Polyunsaturated Fatty Acids 

Milan Čertík, Zuzana Adamechová, Linda Németh 

Department of Biochemical Technology, Faculty of Chemical and Food Technology, 

Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic, 

milan.certik@stuba.sk 

Polyunsaturated fatty acids (PUFAs) with their unique healthy a dietary properties 

have a number of uses in biomedical and nutritional areas. Due to inadequacy 

of their natural sources, new biotechnological approach for PUFA production 

based on solid state fermentation has been developed. During the process, selected 

filamentous Mucorales fungi effectively transform various agroidustrial materials 

to bioproducts enriched with PUFAs, such as gamma-linolenic acid (GLA), dihomogamma-linolenic 

acid (DGLA), arachidonic acid (AA) and eicosapentaenoic acid 

(EPA). Such natural PUFA-bioproducts may be used as an inexpensive food and 

feed supplement and may create new perspectives for the economic competitiveness 

and market of microbial PUFAs. 

Key words: polyunsaturatedc fatty acids, biotechnology 

2.


Lectures 

2-L04 Emerging Technologies in Food Analysis 

2. 


Institute of Chemical Technology Prague, Department of Food Chemistry and Analysis, 

Technicka 5, 166 28 Prague 6, Czech Republic; 

jana.hajslova@vscht.cz 

Recent advances in science and technology have enabled the introduction of 

several novel analytical strategies, which are applicable both in food research and 

routine laboratory control. Time of flight mass spectrometry (TOF MS) represents 

one of the challenging techniques in this field. The pilot studies listed below illustrate 

its potential in analysis of various food attributes including safety, compliance to 

legislative requirements and authenticity. In addition to instrumental techniques, 

bioanalytical procedures are very promising; examples documenting their advantages 

will be presented as well. 

LC-HRTOF MS in pesticide residue analysis - over 200 analytes determined 

within 8´, 3 identification points available; non-target search is possible 

GCxGC / HSTOF MS in analysis of PAHs in plant oils - detection limits and 

other performance characteristics depend on a type of oil and way of its processing, 

validation for each matrix needed 

Ambient mass spectrometry employing Direct Analysis in Real Time (DART) 

source - profiling of various food commodities by a new non-contact surface sampling 

technique allows identification of a source of their origin 

Biosensors - instead of quantification of individual health promoting/toxic 

components, measurement of their biological effects provides information on health 

risks for consumers 

key words: food quality and safety, authenticity, TOF MS, biosensors


Lectures 

2L-05 Physiological Regulation of Biotechnological 

Production of Carotenoid Pigments 

Vladimíra Hanusová, Martina Čarnecká, Andrea Halienová, Milan Čertík, 

Emília Breierová, Ivana Márová 

Department of Biochemical Technology, Faculty of Chemical and Food Technology, 

Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic, 

vladka.hanusova@gmail.com 

Huge commercial demand for natural carotenoids has focused attention on 

developing of suitable biotechnological techniques for their production. A range 

of yeast species utilizing agroindustrial substrates have been tested for carotenoids 

formation. Yeast strains of Rhodotorula and Sporobolomyces formed β-carotene as 

the main pigment together with torulene and torularhodine as minor carotenoids. 

In contrast, Phaffia strains accumulated astaxanthin as a principal carotenoid. To 

increase the yield of these pigments, physiological regulation of the fermentation 

process has been employed. Molecular changes in yeast cells on genome, proteome 

and metabolome level were studied using PFGE, 2D-GE, LC/MS/MS and EPR 

techniques. These studies also revealed that activated biosynthesis of carotenoids by 

yeasts exposed to stress could be in a part explained by their scavenger characters as 

a protection against the harmful effect of the environment. 

Key words: carotene pigments, yeast 

2.


Lectures 

2-L06 Enzymatic Saccharide Acetylations 

in Water – Comparation of Catalysts 

2.10 

Vladimír Mastihuba, Tatiana Kraková, Mária Mastihubová 

Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences 

Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic 

chemvrma@savba.sk 

Enzymatic esterification of various hydroxy compounds is studied for about 

40 years. Such reactions are usually performed in reaction systems comprising controlled 

amount of water to prevent product hydrolysis. We had recently described 

rather unique esterase from Trichoderma reesei catalyzing acetylation of several 

carbohydrates and alcohols in bulk water phase. Our present work explores performance 

of several enzymes/crude enzyme preparations and proteins (Celluclast, 

Ultraflo, lipases from Candida antarctica, Candida rugosa, Aspergillus niger and 

Thermomyces lanuginosus, bovine serum albumin) in acetylations of glucose and 

alpha metyl glucoside, comparing product yields and position selectivity of esterification. 

According to obtained results, Celluclast, a cellulase preparation from 

T. reesei comprising acetyl esterase, seems to be the best catalyst for this reaction 

according to its selectivity, product yields, availability and price. 

Keywords: biocatalysis, acetylations in water


Lectures 

2-L07 Preparation of Bio-Diesel from Tall Oil 

Radoslav Mikulášik, Ján Cvengroš, Viktor Milata, Martin Polovka, 

Svetozár Katuščák, Igor Šurina 

Department of Chemical Technology of Wood, Pulp and Paper, 

Faculty of Chemical and Food Technology, Slovak University of Technology 


radoslav.mikulasik@stuba.sk 

This paper concerns the preparation of bio-diesel from tall oil. It is known that 

tall oil contains substituted fatty and resin acids, sterols and other substances, but 

only the fraction of fatty acids can be used as bio-fuels. Therefore is needed suitable 

method for separation of fatty acids from other components of tall oil. 

There are two possibilities: (1) vacuum distillation of tall oil, followed by the 

esterification of fatty acids fraction to methyl esters, (2) esterification of tall oil to 

methyl esters, followed by vacuum distillation and isolation of fraction of fatty acids 

methyl esters. Both mentioned methods were compared in this work. 

Tall oil was prepared from feedstock (tall soap) by acidification with sulphuric 

acid. Fuel properties of prepared bio-diesel were tested in accredited laboratory. The 

prepared bio-fuel from tall oil accomplishes all parameters of EN 14214 except the 

content of sulphur. 

Keywords: bio-diesel, methyl ester, fatty acids, tall oil, tall soap, esterification, 

vacuum distillation, GC-MS 

2.11


Lectures 

2-L08 Artificial Neural Networks in Food Analysis 

2.12 

Ján Mocák, Viera Mrázová, Dáša Kružlicová, Filip Kraic 


University of Ss. Cyril & Methodius, SK-91701 Trnava, Slovakia. 

jan.mocak@ucm.sk 

Artificial neural networks (ANN) are used more and more frequently in chemistry. 

They have been applied for various purposes: optimisation, quantification of 

unresolved peaks, estimation of peak parameters, estimation of model parameters, 

etc. An important application area of the ANN in food chemistry concerns sample 

characterization and classification. 

The use of the ANN for data and knowledge processing is characterized by 

analogy with biological neurons. The ANN consists of neurons sorted in an input 

layer, one or more hidden layer(s) and an output layer. The input neurons accept 

the input data characteristic for each observation, the output neurons provide predicted 

value or pattern of the studied objects. The neurons of two adjacent layers are 

mutually connected and the importance of each connection is expressed by weights. 

During the training process the weights are corrected so as to produce output values 

as close as possible to the desired (or target) values. After completing the training 

process the network will give predictions for categorization of new objects, not included 

in the training set. 

In this work we have studied the possibility of the ANN application to the classification 

of (1) Slovak varietal wines using different classification criteria (variety, 

producer/location, the year of production), (2) drinkable waters (as the tap-, mineral-, 

mineral carbonated-, spring-, and spring carbonated water). 

Acknowledgement: The support of this work by the grants VEGA 1/3584/06 

and APVV-0057-06 is highly acknowledged. 

Keywords: artificial neural networks, food classification


Lectures 

2-L09 Detection of Spices‘ Irradiation by Modern 

Spectroscopic Techniques 

Martin Polovka, Milan Suhaj 

Department of Chemistry and Food Analysis, VÚP Food Research Institute 

Priemyselná 4, P. O. Box 25, SK-824 75 Bratislava, Slovak Republic 

polovka@vup.sk 

Different methods have been used to reduce the microbial contamination in 

spices, from which γ-irradiation is one of the most effective. In order to eliminate 

any health risks for consumers, exact studies of γ-irradiation treatment and its impact 

on food safety is of high importance. In this contribution, the changes induced 

in ground caraway (Carum carvi, L.) and bay leaves (Laurus nobilis, L.), γ-irradiated 

using 60 Co source at doses from 5 up to 30 kGy were studied using EPR spectroscopy. 

The influence of absorbed dose on the character of formed paramagnetic structures, 

as well as their and life-time were also investigated. In addition, the influence 

of γ-irradiation on antioxidant activity of spices’ alcoholic extracts and its alterations 

upon the storage was investigated by both EPR and UV-VIS spectroscopy, applying 

several common assays (e.g, 2,2-diphenyl-1-picrylhydrazyl ( • DPPH) free radical, 

2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) cation radical (ABTS •+ , determination 

of ferric ions reducing power (FRP), total phenolic content (TPC) and thiobarbituric 

acid reactive substances content (TBARS). 

Keywords: γ-irradiation, spices, free radicals, antioxidant properties 

2.1


Lectures 

2-L10 Application Potential of Novel GC-HT TOF MS 

(TRU TOF) System in Food and Environmental 

Analysis 

2.1 

Jakub Schurek, Jana Pulkrabova, Jana Hajslova 

Instituteof Chemical Technology in Prague 

Technická 5, Praha 6, 166 28 

jakub.schurek@vscht.cz 

In last years, analytical approaches employing gas chromatography coupled to 

time-of-flight mass spectrometry (GC-TOF MS) proved to be a useful tool in assessment 

of quality and safety of food, beverages and water. But only recently, at the end 

of 2007, new TOF MS detector specialy designed for high-throughput of samples, 

has been introduced. 

In this work the evaluations of such instrumentation is performed in analysis of 

pesticides, pharmaceuticals and polybrominated compounds. The ionization mode 

(EI, CI) and GC-MS settings has been optimized in order to obtaine fast and reliable 

analytical methods for routine control of purified extracts of different food-stuffs 

and enviro-matrices. Appraisal of mentioned technique with respect to the cost and 

time demands was also done. 

Keywords: high throughput gas chromatography time-of-flight mass 

spectrometry (GC-HT TOF MS)

2-L11 Cofffe as a Source of Antioxidants 

(An EPR Study) 

Andrej Staško, Vlasta Brezová and Anna Šlebodová 


Lectures 

Institute of Physical Chemistry and Chemical Physics, 

Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, 


andrej.stasko@stuba.sk 

Antioxidant abilities of commercially available ground and instant coffees 

brews were investigated using as oxidants semi-stable radicals as ABTS•+ , DPPH, 

•– • TEMPOL, and also more reactive radicals (SO and OH) formed in the thermally 

4 

decomposed K 2 S 2 O 8 . Total phenolics content was determined. Kinetic behaviour of 

caffeine and caffeic acid was investigated employing as oxidant • OH radicals photochemically 

generated from H 2 O 2 . 

The antioxidant capacity expressed in TEAC is by the instant coffees with 0.71 

± 0.07 mmol g –1 about 3-4 times higher than by the ground coffees 0.22 ± 0.02 mmol g – 

1 . Only minor differences (not exceeding ± 15 %) are evident between the samples in 

the group of instant or ground coffees. Phenolics content correlates well with TEAC 

values as found with DPPH and ABTS •+ oxidants investigating the ground coffees. 

Only limited mutual correlations are evident between other investigated variables 

documented with correlation matrix. 

Caffeic acid (CA) is a very active antioxidant in all investigated systems, whereas 

caffeine (CF) is inert e.g. to ABTS •+ and DPPH. But, both are effective in the scavenging 

of • OH radicals with bimolecular rate constants k CF = 2.6×10 9 M –1 s –1 and k CA = 

9.4×10 9 M –1 s –1 . 

Keywords: ground and instant coffees, antioxidants, EPR 

2.1


Lectures 

2-L12 Interactions Between Organic Food 

Contaminants and Plastic Packaging Materials 

2.1 

Peter Šimko, Božena Skláršová, Peter Šimon, B. Drobná 

Food Research Institute, 

Priemyselna 4, P. O. Box 25, 824 75 Bratislava 

Interactions of two groups of hazardous contaminants - polycyclic aromatic 

hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) with plastic packaging 

materials were studied. 

Sunflower and rapeseed oils were spiked with benzo[a]pyrene (BaP). Then, the 

oils were filled into polyethylene terephtalate (PET) and low density polyethylene 

(LDPE) cylindrical shape receptacles and BaP concentrations were followed within 

49 h using HPLC with fluorescence detection. It was found that the concentration in 

the oils in contact with PET were decreased considerably due to sorption of BaP onto 

PET, while no decrease in concentration of BaP was observed in the oils in contact 

with PE. It was possible to describe the rate of BaP removal by kinetic equation and 

calculate diffusion coefficients. The results showed that PET is able to decrease BaP 

concentration in both vegetable oils and the decrease is due to monolayer sorption 

processes taking place on the PET surface. As found, LDPE is inappropriate material 

for BaP removal from sunflower and rapeseed oils, because BaP concentration in the 

oils remained at a constant level during the whole experiment. 

Then the rapeseed oil was spiked with 6 indicator PCBs congeners and filled 

into PET cylindrical shape receptacles. PCBs concentrations were followed within 

48 h using GC/ECD. However, in this case we did not detect a remarkable decrease 

of PCBs concentration. As found, PET is inappropriate material for PCBs removal 

from rapeseed oil. 

Keywords: polycyclic aromatic hydrocarbons, polychlorinated biphenyls


Lectures 

2-L13 Analysis of Fusarium Mycotoxins, Critical 

Assessment 

Zachariasova Milena, Hajslova Jana, Poustka Jan, Kostelanska Marta, 

Krplova Alexandra, Vaclavik Lukas 

Institute of Chemical Technology in Prague 

Technicka 3, Prague, 166 28 

milena.zachariasova@vscht.cz 

Fusarium toxins, secondary metabolites produced by Fusarium fungi, occur 

worldwide as natural contaminants in cereals, including wheat, barley, maize and 

oats. With regard to health risk for consumers, their presence in cereals and processed 

products has to be monitored. 

Gas chromatography coupled to either electron capture or mass spectrometric 

detector used to be frequently employed method in mycotoxins analysis. This approach 

requires intensive sample clean-up and time consuming derivatization step, 

therefore, in recent years, LC–MS/MS has become a popular tool in Fusarium toxins 

control (direct target analysis of crude or purified extract is feasible). For non target 

screening, the use of LC coupled to high-resolution time-of-flight MS (HRTOFMS) 

is a challenging option. Based on the accurate mass measurement, identification 

of various “masked” mycotoxins such as deoxynivalenol-3-glucoside (DON-3-Glc) 

and other conjugates is possible. As shown in our recent studies, these compounds 

cross-react in enzyme linked immunosorbent assay (ELISA). While poor trueness of 

data due to DON-conjugates cross-reactivity may pose a problem, this phenomenon 

can be employed for their pre-concentration prior to instrumental analysis. 

Keywords: Fusarium toxins, LC-MS/MS, LC-TOF-MS, ELISA 

2.1

2.1

Posters 

2.1

2.20


Posters 

2-P001 Determination of β-Carotene in the Gelatin 

Capsule 

B. Altangerel, Z. Kolajova, D. Kramarova, O. Rop, I. Hoza 

Department of Food Engineering, Faculty of Technology, 

TBU in Zlin, Czech Republic 

kramarova@ft.utb.cz 

A reversed-phase high-performance liquid-chromatographic method for the 

determination of β-carotene is described. This method was applied to β-carotene 

measurement in gelatin capsule. Filling from gelatin capsule was dissolved into 10 

ml of deionized water and extracted using 10 ml of n-Hexan solution. After evaporation, 

the residue was dissolved in 10 ml of ethanol and was filtered trough 0.45 

μm filter (Nylon) before injected into the column. HPLC separation was performed 

using ESA HPLC with ECD detection (Coulochem III) which is equipped with analytical 

column Supercosil LC18-DB (250 x 4.6mm, particle size 5μm). Separation 

took place at 30°C, flow rate of mobile phase (methanol: acetonitrile: phosphoric 

acid) was 1.0 ml.min-1. Potentials of the cells for detector were 500 and 600mV. 

The result of this study shows that the gelatin capsule vitamin supplement analyzed 

here contains 5.83± 0.02 mg of β-carotene, which is different from the labelled 

value of 6.00 mg. However, the significance of the difference was not ascertained. 

The validation of the analytical method used in this study was performed using 

standard addition technique. Therefore, it can be concluded that HPLC with ECD 

method have shown to be acceptable for determination and quantification of β-carotene 

in a gelatin capsule vitamin supplements. 

Keywords: gelatin capsule, β-carotene, HPLC, Coulochem III, ECD 

2.21


Posters 

2-P002 Determination of β-Carotene in Tomato by 

High Performance Liquid Chromatography with 

Electrochemical Detector 

2.22 

B. Altangerel, P. Vojtiskova, D. Kramarova, , O. Rop, I. Hoza 

Department of Food Engineering, Faculty of Technology, TBU in Zlin, Czech Republic E-mail: 

kramarova@ft.utb.cz 

This study was conducted to investigate the antioxidant vitamin (β-caroten) 

content of one of the most important vegetables, tomato, using modern analytical 

techniques. High performance liquid chromatography (HPLC) is the most commonly 

used method for the separation, quantitation, and identification of carotenoids 

found in vegetables samples. 

Initially a 10 g sample of tomato was placed in a 50 ml flask and mixed with 26 

ml of extraction solvent (acetone: hexane 50:50v/v). After filtration through Filtrak 

No.390 filter paper. The filtrate was poured into a 250 ml flask and evaporated under 

vacuum at 30 o C and residues were redissolved in 5 ml of ethanol. The solution was 

filtrated through a 0,45 μm nylon filter and 20 μl was injected into HPLC system. 

HPLC separation was performed using ESA HPLC with ECD detection (Coulochem 

III) which is equipped with analytical column Supercosil LC18-DB (250 x 4.6mm, 

5μm) with methanol: acetonitrile: phosphoric acid (70:29.9:0.5 v/v/v) as mobile 

phase in a isocratic elution. 

The first group sample was immediately extracted with extraction solution 

(hexan:ethanol, 50:50 v/v) after purchased from supermarket. The second group 

sample was stored two weeks in a refrigerator before extraction. The higher value of 

β-carotene (1,197 mg/100g -1 ) was obtained with the first sample. The values obtained 

in this study are very close to those reported by Olives Barba et al. who reported a 

range of 0.6-1.2 mg per 100 g for β-carotene in 4 tomato varieties. We presumed that 

the content of β-carotene (0.024mg/100g -1 ) was reduced in second sample during 

storage period. 

European epidemiological studies revealed 6-10 mg β-carotene as the recommended 

daily intake to provide good health and a reduction in risk of diseases 

(Lachance, 1998; National Research Council, 1989). Accordingly, one serving 100 g 

tested tomato contributes 11.97-19.95% of the recommended daily intake of β-carotene. 

Keywords: β-carotene, tomato, HPLC, Coulochem III, ECD


Posters 

2-P003 Protective Effect of Tomatoes Powder 

in Oxidative Stress Induced by Thyroid 

Disfunctions 

Sanda Andrei, Adela Joanta, Adela Pintea, M. Merca Sarlea 

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca 

Manastur, 3-5, 400372,Cluj-Napoca, Romania 

sandrei@usamvcluj.ro 

Accumulating evidence has suggested that the hyperthyroidism is associated 

with increases in radical oxygen species (ROS) production and lipid peroxidation 

products in some tissues of rats. 

In the present work, effects of the ingestion of tomatoes powder, rich in lycopene, 

on the activities of antioxidant enzymes (superoxid dismutase, catalase and glutathion 

peroxidase), the oxidation of lipids and on fatty acids (FA) composition on 

liver, thyroid and myocardium were examined in rats intraperitonial injected with 

L-thyroxin. Enzyme activities were determined from protein extract obtained from 

tissues. Enzymes activities and total protein content was assayed using the photometric 

methods. Lipid peroxides level was determined measuring TBARS test. The 

fatty acids analysis was made on total lipids extracts using gas chromatography 

coupled with mass spectrometry (MS). 

Tomatoes powder supplementation reduces the burden of oxidative stress in 

hyperthyroidism. Administration of this powder rich in lycopene in hyperthyroid 

rats led to reduction in lipid peroxidation and susceptibility to oxidative stress and 

to increase in antioxidant level. These diet determined also an increase of unsaturated 

/ saturated fatty acids ratio in all analyzed tissues. L/thyroxin administration 

determined the modification of FA content in thyroid gland, in liver and also in 

myocardium. 

Keywords: antioxidants, hyperthyroidism 

2.2


Posters 

2-P004 Thermophilic Bacteria Application to Whey 

Biodegradation 

2.2 

Libor Babák 

Institute of Food Science and Biotechnology, Faculty of Chemistry, 



babak@fch.vutbr.cz 

A biodegradation possibility of the organic waste compounds (summarized 

like chemical oxygen demand – COD) by means of the mixed thermophilic aerobic 

bacteria culture (sludge of wastewater treatment Bystřice pod Hostýnem) was studied. 

The whey from Pribina Přibyslav company is the tested waste medium. 

In this work a batch system was used. The cultivations were practised in the 

laboratory fermentor BIOSTAT B ( B. Braun Biotech) at 60 ºC. Selected bioengineering 

characteristics (specific growth rate, biomass productivity, COD elimination 

and volume ogygen transfer coefficient) were determined persuant to the experimental 

data. It was achieved 62 ± 4 % total COD reduction after two main growth 

phases. 

Keywords: whey, biodegradation, thermophilic


Posters 

2-P005 Growth Curves of Mixed Thermophilic Bacteria 

Libor Babák, Radka Burdychová, Vlastimil Dohnal 




babak@fch.vutbr.cz 

The growth of mixed thermophilic bacteria culture (sludge of wastewater treatment 

Bystřice pod Hostýnem) was studied. Two different cultivation systems were 

used for monitoring and comparison of growth characteristics of above mentioned 

thermophilic culture. The first system consisted of batch cultivations in cuvettes 

with very little volumes (10 -5 L) of cultivation media at 60 ºC. Samples were taken by 

autosampler in periodic intervals (24,4 min). Bacteria growth was there observed by 

means of HPLC with UV detection (600 nm). The second system was composed of 

batch cultivations in the laboratory fermentor BIOSTAT B ( B. Braun Biotech) at 60 

ºC, too. The system was aerated (10 L.min -1 ) and stired by multiple turbo-stirrer (250 

min -1 ). Samples were taken every two hours. Biomass concentration was detected by 

turbidimeter. Both cultivation ways were practised in several substrates with different 

C-sources (glucose, sacharose, lactose, maltose). Obtained growth curves were 

comparised. Furthermore, the biomass productivity values were determined. 

Keywords: thermophilic, growth curve, HPLC/UV, bioreactor 

2.2


Posters 

2-P006 Monitoring of Acrylamide Levels Using LC-MS/ 

MS: Malts and Beers 

2.2 

Veronika Bartackova, Lukas Vaclavik, Katerina Riddellova, 

Jana Hajslova 

Institute of Chemical Technology Prague, Department of Food Chemistry and Analysis 

Technicka 3, 166 28 Prague 6 – Dejvice, Czech Republic 

veronika.bartackova@vscht.cz 

Alike many others heat processed food products prepared from raw materials 

rich in starch/reducing sugars, malts may contain acrylamide, a toxic processing 

contaminant originated from precursors contained in barley. Besides of other factors, 

acrylamide levels depend on the temperature and the time employed for treatment 

of respective products. In our study, various types of malts, commonly used as 

additives (colorants, aroma donors etc.) in bakery and/or in brewing industry were 

analysed. In this study, we focused on the assessement of transfer of acrylamide into 

the beer. For this purpose, a new analytical strategy enabling direct determination of 

acrylamide in this matrix has been developed. The procedure consists from cleanup 

step employing solid-phase extraction (SPE) followed by liquid chromatography 

coupled to tandem mass spectrometry with a triple quadrupole mass analyser 

(LC–MS/MS). Isotope dilution technique employing 13 C 3 -acrylamide as an internal 

standard was used for quantification of native acrylamide. A wide range of beers 

obtained at Czech markets were examined within this monitoring study. 

Keywords: acrylamide, malt, beer, LC–MS/MS


Posters 

2-P007 Determination of Polycyclic Aromatic 

Hydrocarbons in Czech Honeys 

Petra Batelková, Ivana Borkovcová, 

Klára Bartáková, Lenka Vorlová 

University of Veterinary and Pharmaceutical Science Brno 

Palackého 1/3, 612 42 Brno 

pbatelkova@vfu.cz 

Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants formed 

during the incomplete combustion of carbonaceous materials at high temperature. 

They have toxic, carcinogenic and mutagenic effects. 

The aim of this study was to determinate the content of PAHs in Czech honeys. 

The samples of honey were obtained both directly from bee-keepers and shops. 15 

PAHs have been estimated in 20 samples of honey by means of reverse phase HPLC 

with gradient elution and fluorescence detection. Target analytes were isolated 

from samples into organic solvents either from their aqeous solutions (liquid/liquid 

extraction or solid phase extraction) or directly with adition of anhydrous sodium 

sulphate by ultrasonic extraction combined with mixing at 10 000 rpm in organic 

solvent. Concentrations of individual PAHs found in honey samples were low, they 

were in range 0,1 – 10 μg.kg -1 . 

Keywords: honey, polycyclic aromatic hydrocarbons, HPLC 

2.2


Posters 

2-P008 Quantitative Determination of Sulfonamide 

Residues in Chicken Meat by a New Solid Phase 

Extraction and 

HPLC-UV for Detection 

2.2 

Constantin Bele, Adela Pintea, Francisc Dulf, Dan Lupu, 

Alexandru R. Biris 

University of Agricultural Science and Veterinary Medicine , 

R- 400372 Cluj-Napoca , Romania 

cbele2002@yahoo.com 

In this study, an analytical method for the simultaneous determination of seven 

of the most used sulfonamides in chicken meat was developed. Sample cleanup 

and pre-concentration of analytical compounds was carried out by employing 

SPE using multiwalled carbon nanotubes and alumina as solid phase adsorbents 

followed by high performance liquid chromatography with UV detector. The HPLC 

determination was performed using a RP C 18 column and sulfonamides were detected 

at 266 nm.The newly developed sample pre-treatment procedure effectively 

removed the potential matrix interferences from endogenous compounds of chicken 

meat. Under the optimal procedures , the detection limits of sulfonamides were in 

the range of 24–42ng L -1 . Average recoveries of analytes from spiked meat ranging 

from 65.6 to 80.4 % were determined. 

Keywords: Solid phase extraction; Multi-walled carbon nanotubes; 

Sulfonamides; HPLC


Posters 

2-P009 Monitoring of Ferulic Acid Content During the 

Malt Production 

Sylvie Běláková, Renata Mikulíková, Zdeněk Svoboda, 

Simona Macuchová 

Research Institute of Brewing and Malting 

Mostecká 7 614 00 Brno Czech Republic 

belakova@brno.beerresearch.cz 

Ferulic acid is a natural antioxidant that belongs to low-molecular weight phenol 

compounds. 

Its antioxidative activity plays an important role in barley and biochemical process 

of beer production. Nowadays, causes of wort turbidity have been investigated 

and relationships between wort turbidity, filterability and beer quality have been 

studied. Information on ferulic acid will help clarify the causes of turbidity. The aim 

is to find out varietal and technological dependence of the intensity of enzymatic 

cleavage of bonds of ferulic acid with proteins and polysaccharides in cell walls during 

barley germination and the effect of this process on the rate and quality of wort 

running-off and beer filterability. 

The optimized method of ultra performance liquid chromatography (UPLC) 

with UV detection was used for the determination of ferulic acid in barley, malt and 

wort. 

Keywords: UPLC, ferulic acid, malt, beer 

2.2


Posters 

2-P010 Monitoring of Sensorially Active Sulphur 

Compounds in Malt and Beer 

2. 0 

Sylvie Běláková, Renata Mikulíková, Zdeněk Svoboda, , 




mikulikova@brno.beerresearch.cz 

Recently, increased attention has been devoted to sensorially active substances 

affecting beer quality. Heterocyclic and sulphur containing compounds, some of 

them with high sensorially activity even in extremely low concentrations, play an 

important role. Trace amounts of these compounds commonly occurring in foodstuffs 

contribute to their flavor and their effect can be generally assessed as favorable. 

However, in malt or beer this is true only to a limited extent and their presence 

is assessed mostly unfavorably. 

The SPME/GC/FPD method was used for following the selected sulphur sensorially 

active substances in beer 

Keywords: sulphur compounds, SPME, GC, FPD, malt, beer


Posters 

2-P011 Content of Strobilurin Fungicides in Barley 

Malt and Beer 

Sylvie Běláková, Renata Mikulíková, Zdeněk Svoboda, 




mikulikova@brno.beerresearch.cz 

Strobilurin pesticides represent an important group of agricultural fungicides 

with a new efficiency mechanism. 

Strobilurins chemically belong to metoxyacrylates (e.g. azoxystrobin and 

picoxystrobin), metoxycarbamates (pyraclostrobin), oximino acetates (kresoxim 

– methyl, trifloxystrobin), ozimino-acetamides (dimoxystrobin, etc.) and dihydrodioxazinons 

(fluoxastrobin). Their characters, including spectrum efficiency, are 

very variable. In plant protection, they act mainly preventively but also curatively 

and some even have eradicative effects. All of them act in contact, some of them in 

depth or systemically and their efficacy is very wide. On the other hand, howver, 

they can pose a risk to human health and potentially endanger the environment. 

Azoxystrobin (Amistar), picoxystrobin (Acanto), trifloxystrobin (Sfera 267.5 

EC) and kresoxim-methyl (Juwel, Juwel Top) are used against fungal diseases of 

barley. Azoxystrobin (Ortiva) has been registered against fungal diseases of hop. 

With respect to the increased use of strobilurin for barley and hop protection 

and due to their stability in a plant, it was necessary to introduce monitoring of strobilurin 

residues in barley, malt and beer. Analytical determination of the selected 

strobilurin fungicides is performed with the GC/MSD method. 

Keywords: strobilurins, pesticides, GC/MS, malt, beer 

2. 1


Posters 

2-P012 The Effect of Fermentation Conditions on the 

Yeast Aroma Profile 

2. 2 

Emília Breierová, Kornélia Nemcová, Jaroslava Kaňuchová Pátková, 

Ingrid Vajciková 

Institute of Chemistry, SAS, 

Dúbravská cesta 9, 845 38 Bratislava, Slovakia 

chememi@savba.sk 

Several yeast strains of Saccharomyces cerevisiae, Rhodotorula mucilaginosa, Pichia 

anomala, Pichia membranofaciens, Sporobolomyces pararoseus, Bretanomyces were studied 

for their aroma compounds production under various conditions –temperature, 

medium composition, microaerobic/anaerobic conditions. All these conditions significantly 

affected the aroma production by all of the yeasts strains. Typical aroma 

compounds for each genus were recognised: valericaldehyd and γ-nonalaktón for 

S. cerevisiae, cyklopentanol, alfacyklogeraniol for Rhodotorula mucilaginosa, sabinylacetate, 

3,4-hexandion and eicosane for Sporobolomyces. There were significant 

differences also between yeast strains from the same genus. S. cerevisiae A has produced 

caproic aldehdyd, trans-pinocamfón, dodecanal, 5-methyl3-heptanón, izomenthylacetát, 

but S. cerevisiae strain B has not produced any of these compounds. 

Rhodotorula A has produced higher amounts of 2,3-butandiolu, but has not produced 

neither izopentylformiát nor 3,4-hexandion, which has strain Rhodotorula B 

produced. 

Keywords: aroma, yeast,


Posters 

2-P013 Biogenic Amines Reduction by Probiotic L. casei 

During Ripening of Fermented Sausages 

Radka Burdychová, Vlastimil Dohnal, Petra Hoferková, Pavla Sládková 

Mendel University of Agriculture and Forestry in Brno 

Zemedelska 1, 613 00 Brno, Czech Republic 

burdycho@node.mendelu.cz 

The aim of this work was to study the effect of probiotic bacteria L. casei and 

the mixture of two different starter cultures (1 – Staphylococcus carnosus, Lactobacillus 

curvatus; 2 – Pediococcus acidilactici) on biogenic amine formation during fermentation 

and ripening of typical Czech fermented sausages. Determination of tryptamine, 

2 – fenyletylamine, kadaverine, histamine, tyramine, spermidine and spermine 

was carried out using HPLC with UV detection. Other parameters like pH, water 

activity, weight loss, total counts of microorganisms, LAB and counts of probiotic 

L. casei were determined. In batches with probiotic L. casei were significantly lower 

concentration of putrescine, kadaverine and tyramine than in two control batches 

without L. casei. Mixtures of starter and probiotic bacteria decreased pH quickly 

which supports the growth of lactic acid bacteria and inhibited the growth of contaminant 

microorganisms in raw material. L. casei was detected at concentration of 

10 6 CFU/g in both testing batches after three days of sausages production and stay 

at this level during the whole fermentation and ripening period. 

Keywords: biogenic amines, L. casei, HPLC, probiotic 

2.


Posters 

2-P014 Testing of Different Sacharomyces Species for the 

Ability to Sorb DeoxynivAlenol 

2. 

Radka Burdychová 

Mendel University of Agriculture and Forestry in Brno 

Zemedelska 1, 613 00 Brno, Czech Republic 

burdycho@node.mendelu.cz 

Deoxynivalenol is the most prevalent mycotoxin produced by Fusarium spp. 

which frequently contaminates cereal crops. There are several studies describing 

that cell walls of yeasts and bacteria are able to comlex with mycotoxins and limit 

their bioavailability in the digestive tract of human and animals. The aim of this study 

was testing of ability of different Saccharomyces species to remove deoxynivalenol 

from liquid medium. HPLC/MS was used for monitoring both the yeasts growth 

and mycotoxin reduction. From six species tested, four was able to sorb from 40 – 68 

% of deoxynivalenol. 

Keywords: mycotoxins, deoxynivalenol, Saccharomyces, sorbtion


Posters 

2-P015 Separation of Complex Oligosaccharides from 

Worth and Beer Using HPLC 

Jana Cabálková, Janette Bobáľová 

Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic v.v.i., 

Department of Proteomics and Glycomics, Veveří 97, CZ-60200 Brno, 

Czech Republic 

cabalkova@iach.cz 

Monitoring of oligosaccharides (OS) during the malting and fermentation processes 

has essential and practical importance for development of brewing technologies. 

The intent of the study was to optimize analytical conditions for separation of 

the OS during the malting process from worth and beer. 

Non-derivatized OS were separated by high performance liquid chromatography 

(HPLC) with refractive index (RI) detection on Prevail Carbohydrate ES HPLC 

analytical column using mixture of acetonitrile/water 3:2 (v/v). Under optimized 

conditions, samples of congress wort, hopped worth, green beer, and beer revealed 

noticeable changes in the quantity of OS for multiple degree of polymerization 

(DP) from di- up to tetradecasaccharides. Low molecular weight OS (up to DP 4) 

showed significant decrease in the samples of green beer compared to the congress 

wort. They were consisted of linear α-(1→4)-linked glucose chains, so were easily 

fermented. On the contrary, high molecular weight OS (above DP 4) did not showed 

any significant changes in their content. They could not be easily fermented due to 

the presence of maltose/maltotriose residues of α-(1→6)-linkages. 

Acknowledgement: Work was supported by the Ministry of Education, Youth 

and Sports, Czech Republic, project No. 2B06037 and Institutional Research Plan 

AV0Z40310501. 

Keywords: oligosaccharides, wort, beer, HPLC 

2.


Posters 

2-P016 Traceability of Honey and Olive Oil Origin using 

SPME–GC×GC–TOFMS 

2. 

Tomas Cajka, Katerina Riddellova, Jana Hajslova 

Institute of Chemical Technology, Department of Food Chemistry and Analysis 

Technicka 3, 166 28 Prague 6, Czech Republic 

tomas.cajka@vscht.cz 

A wide range of volatile compounds occurs in food headspace, including components 

responsible for typical flavour, off-flavour, and other quality/safety parameters. 

For the characterisation of volatiles, solid-phase microextraction (SPME) in 

combination with gas chromatography–mass spectrometry (GC–MS) represents a 

conceivable method of choice. Typically, conventional one-dimensional GC is used 

for the separation of volatiles. However, due to the complexity of aroma, co-elution 

of some volatiles may occur. Under these conditions, comprehensive two-dimensional 

GC (GC×GC), that separates compounds using two columns with fundamentally 

different separation mechanisms, represents a very promising tool for food 

profiling and other applications aimed at characterisation of a sample. In addition, 

application of time-of-flight MS allows obtaining full spectral information and also 

fast acquisition rates needed for a proper reconstruction of very narrow chromatographic 

peaks generated by GC×GC. 

In this presentation, application of SPME–GC×GC–TOFMS for the examination 

of volatile compounds in honey and olive oil samples will be discussed with emphasis 

on traceability of origin. 

Acknowledgements: This study was carried out within the projects FP6-FOOD- 

2004-006942–TRACE and MSM 6046137305. 

Keywords: volatile compounds, food analysis


Posters 

2-P018 Extention of Asparaginase Application to 

Acrylamide Minimization from Potato to Cereal 

Products 

Zuzana Ciesarová, Kristína Kukurová, Alena Bednáriková 




The enzymatic way of acrylamide reduction seems to be a perspective option 

to the acquirement of higher safety of several heat-treated potato and cereal products. 

The main precursor of acrylamide formation is known to be, besides reducing 

saccharides, amino acid asparagine. The application of enzyme asparaginase leads 

to the avoidance of acrylamide formation due to the conversion of asparagine to 

aspartic acid which results in a less occurrence of acrylamide in final food products. 

The asparaginase application was successfully demonstrated in potato-based products 

with addition of enzyme solution to the dough and no alterations in sensory 

properties were observed. The more extensive mitigation of acrylamide level is supposed 

to be attained at optimised conditions of enzyme application and heat treatment. 

Similarly, the enzyme utilization was enlarged to the cereal-based products, 

especially in gingerbread and home-made sweet fried cakes production which was 

proved on a simulated model matrix. 



Keywords: acrylamide, asparaginase 

2.


Posters 

2-P019 Amino Acids Profile of Selected Wholegrains 

Important to Acrylamide Formation in Cerealbased 

Products 

2. 

Zuzana Ciesarová, Kristína Kukurová, Alena Bednáriková, 

Peter Hozlár 




Acrylamide as a suspected carcinogen attracts great attention due to its widespread 

occurrence in many staple foods of daily usage as well as due to the 

recommendation of the European Commission to minimize its level. It is known 

that acrylamide arises from naturally occurred compounds in plants such as reducing 

saccharides and amino acids during the commonly used process of heat treatment 

of foods. In the cereal sector the main way of acrylamide reduction is through 

amino acids control responsible for acrylamide formation. Acrylamide is preferably 

formed from amino acid L-asparagine, only in a less extent from aspartic acid, glutamine, 

and glutamic acid in the presence of saccharides at baking. From the point 

of view of acrylamide formation, the selected 10 sorts of wholegrains appointed for 

human usage in bread and cereal breakfast production were assorted according to 

profile of amino acids mentioned above. 



Keywords: acrylamide, asparagine


Posters 

2-P019 Characterization of Beta-Carotene Enriched 

Biomass Production by Red Yeasts 

Martina Čarnecká, Andrea Hároniková, Terezie Dvořáková, 

Andrea Halienová, Ivana Márová, Emília Breierová 

Brno University of Technology, Faculty of Chemistry , Department of Food Chemistry and 

Biotechnology, Purkyňova 118, 612 00 Brno 

e-mail: carnecka@fch.vutbr.cz 

During the last decades fast progress has been made within the field of biochemistry 

of carotenoid biosynthesis in bacteria, fungi, and plants. Although more 

than 600 different carotenoids have been identified in nature, only a few are used 

industrially. There are many yeast strains able to produce different carotenoids. 

However, the heterobasidiomycetous yeast Xanthophyllomyces dendrorhous, are the 

only microbial systems with commercial potentials for the production of astaxanthin. 

In this work three different carotenogenic strains (Sporobolomyces roseus., 

Rhodotorula glutinis, Rhodotorula rubra) were enrolled into a comparative study. 

Each strain was cultivated at optimal growth conditions and in medium with modified 

carbon and nitrogen sources. Complex substrates as well as some waste materials 

(whey, potato extract) were used as nutrient source. Some types of exogenous 

stress were applied too. The production of carotene-enriched biomass was carried 

out in flasks as well as in laboratory fermentor. As the best producer of enriched biomass 

strain Rhodotorula glutinis CCY 20-2-26 was found. Production of biomass in 

R.glutinis was about 35 g/l. Total carotene enrichment of this biomass about 2.5 mg/g 

d.w. was obtained. Significant changes in biomass as well as carotenoid production 

were observed in individual strains according to cultivation conditions. 

Keywords: carotenoids, Rhodotorula, Sporobolomyces 

2.


Posters 

2-P020 Monitoring of Polycyclic Aromatic 

Hydrocarbons and Pesticides Occurring in Oils 

and Fish Products 

2. 0 

Lucie Drábová, Marie Suchanová, 

Vladimír Kocourek, Jana Hajšlová 

Department of Food Chemistry and Analysis, ICT, Prague 

Technická 3, CZ-16628 Prague 6, Czech Republic 

lucie.drabova@vscht..cz 

A wide range of organic contaminants may occur in canned fish and sea products. 

Both components of animal origin and plant ingrediences such as olive oil, 

vegetables, spices etc., may contain hazardous chemicals. 

In this study, 20 polycyclic aromatic hydrocarbons (PAHs) (both EU and EPA 

priority list), persistent halogenated contaminants represented by DDT, HCB, PCBs 

and BFRs, together with 112 modern pesticides were determined in all together 40 

samples collected at Czech market. 

Pressurized liquid extraction (PLE) was employed for analyte isolation followed 

in the next step by automated gel permeation chromatography (GPC) used 

for remaining major matrix coextracts. Quantification of target contaminants was 

carried out by either GC/MS (single quadrupole) or GCxGC – TOF MS.The data 

obtained in this study will be critically assessed with regard to performance characteristics 

of analytical performance and occurrence of target chemicals in examined 

samples. 

The study was carried out within the project MSM 6046137505 supported by 

the MSMT of the Czech Republic 

Keywords: GC/MS, PLE, organic contaminants


Posters 

2-P021 Biogenic Amines in Cheeses and Human Health 

Eva Dudriková, Zuzana Dičáková 

University of Veterinary Medicine 

Komenského 73, 041 81 Košice, Slovak Republic 

dicakova@uvm.sk 

Biogenic amines are low molecular weight alkyl- or aryl-substituted derivatives 

of ammonia. The substituents can have aliphatic, aromatic or heterocyclic structures. 

The number of alkyl or aryl groups attached to the nitrogen determines whether 

the amine is primary, secondary and tertiary. The most important ones from the 

food hygienic point of view are primary amines. Fresh milk usually contains very 

low levels of biogenic amines. However, some microbes found in raw milk or used 

as starter cultures possess decarboxylating enzymes, which can convert free amino 

acids to potentially toxic biogenic amines. Cheese is the most commonly implicated 

food item associated with biogenic amine food poisoning. The matured cheeses are 

the typical dairy products that have caused biogenic amine intoxications. Due to 

the great variety of individual sensitivity and the effect of potentiating factors, it is 

not always easy to establish the acceptable levels of amines in foods. Furthermore, 

the effects of each biogenic amine vary and it is not clear whether the limit values 

should be set for individual amines, for the sum of the toxic of all amines detected. 

Until now, there have been no official limits for the concentrations of biogenic amines 

in dairy products; the limit value of 200 mg.kg -1 has been given only for the 

tyramine in hard cheeses. Approximately 1 000 mg.kg -1 is supposed to elicit toxicity. 

It is generally known, that from a good manufacturing practice viewpoint, levels of 

50 – 100 mg.kg -1 histamine, 100 – 800 mg.kg -1 tyramine, or a total of 100 – 200 mg.kg -1 

are regarded as acceptable. The reported ranges of biogenic amines in cheeses have 

varied from not detected to 2 500 mg.kg -1 histamine, 2 210 mg.kg -1 tyramine, 1 390 

mg.kg -1 cadaverine and 684 mg.kg -1 putrescine. 

Cadaverine (CAD), histamine (HIS), tyramine (TYR) and putrescine (PUT) 

were determined in 31 commercial cheeses commonly consumed in Slovakia (high 

cooked cheese, like Emmental, n = 5; low cooked cheese, like Edam, n = 8; mozzarella 

like cheese, n = 9; mould cheese, n = 5, and sour natural ripened cheese containing 

yeasts and Brevibacterium linens, n = 4), using ion-exchange chromatography. Limit 

of detection for all determined biogenic amine was 0.5 mg.kg -1 and limit of quantification 

was 1.0 mg.kg -1 , respectively. Chemical analyses were carried out in parallel 

with determination of sensory assessment of cheeses and their microbiological ... 


2. 1


Posters 

2-P022 Phytosterol and Fatty Acid Profile of Four 

Edible Functional Oils Processed in Romania 

2. 2 

Francisc Vasile Dulf, Carmen Socaciu, Constantin Bele, Adela Pintea, 

Mihaela L. Ungureşan 

University of Agricultural Sciences and Veterinary Medicine, 

3-5 Mãnãstur Str., 400372, Cluj-Napoca, Romania, 

francisc_dulf@yahoo.com 

In seed (kernel) oils triacylglycerols are the main constituents making up about 

95–98% of the oils. The remaining nonglyceridic fraction (2–5%) consists of different 

compound classes such as sterols (free and esterified), pigments, hydrocarbons, 

tocopherols, fatty alcohols, etc. 

Four functional oils: walnuts, sesame, peanut and poppy seed oil, processed 

by minimal technologies (cold pressing) in Romania were investigated. The goal 

of the present study was the identification and quantification of fatty acids as FA- 

MEs (fatty acid methyl esters), in the total lipid extracts and in the esterified sterol 

fractions. Free and esterified sterols were also quantified as trimethylsilyl ether 

derivatives, using gas-chromatography (GC) with flame ionization detector (FID). 

The main fatty acids found in both of the studied fractions of these four edible oils 

were: palmitic, stearic, oleic and linoleic acid at different concentrations, depending 

on the oil type. The total phytosterol profile was also different, the predominant 

phytosterols in all vegetable oils being β-sitosterol, campesterol and ∆ 5 -avenasterol. 

In walnut and sesame oil the dominant form of sterols was the free one. The total 

sterol concentrations ranged from 182 mg/100g oil (peanuts oil) to 545 mg/100 g oil 

(sesame oil). 

Keywords: edible oils, fatty acids, sterols, GC-FID


Posters 

2-P023 Antioxidant Activity of Flavanols From Grape 

Seed Extracts 

Rugina Dumitrita, Simona Vicas, Carmen Momeu, Carmen Socaciu 

University of Agricultural Sciences and Veterniary Medicine, 

Cluj -Napoca, Manastur street, No.3-5, postal code 400372, 

oliviapreda@gmail.com 

Flavonoids belong to polyphenols class, being plant secondary metabolites. One 

of the most interesting subclass of flavonoids is flavanols, encountered in grapes, 

exhibiting antioxidant properties. Flavanols from four Romanian grape seed varieties: 

Merlot, Feteasca, Mustoasa, Chasla were extracted and the antioxidative capacity 

was determined by DPPH, TEAC, ORAC and Folin Ciocalteu assays. 

DPPH assay and TEAC assay show that Merlot grape seed extract has an antioxidative 

potential 7.6 mM Trolox Eq/g, respective 8 mM Trolox eq/g sample, when 

extracts contain 80% flavanols (HPLC determination). Mustoasa grape seed extract 

has the antioxidative effect 2.2 mM Trolox Eq/g grape seed powder, respective 5 mM 

Trolox Eq/g sample, when extracts contain 45% flavanols (HPLC determination). 

ORAC assay shows the following ranking: Merlot > Mustoasa ~ Feteasca > 

Chasla (in the assay is used a neutral pH close to in vivo pH). The same ranking of 

antioxidant potential was obtained also with Folin Ciocalteu assay. 

Merlot, Mustoasa, Feteasca varieties have a high reductive capacity of three 

different radicals (DPPH . ; ABTS . ; peroxyl). 

These antioxidant assays values could not be compared among themself, but 

can be easely corelated with Folin Ciocalteu assay. 

Keywords: antioxidant, grape seeds. 

2.


Posters 

2-P024 Influence of Long-Term Storage Onditions 

on Antioxidant and Other Active Component 

Content in Several Sorts of Apples 

2. 

Kateřina Duroňová, Kateřina Pařilová, Andrea Halienová, Jitka Dvořáková, 

Radka Kočí, Jan Goliáš, Ivana Márová 



xcduronova@fch.vutbr.cz 

Apples are one of the most common sources of natural antioxidants in Czech 

population. This work was focused on study of changes of antioxidant content, enzyme 

activites, protein and composition in several local sorts of apples stored for a 

long time at controlled atmosphere with reduced oxygen content. To qualitative as 

well as quantitative analysis of individual low-molecular weight antioxidants RP- 

HPLC/UV-VIS and LC/MS were used. Activites of superoxide dismutase, peroxidase 

and polyphenoloxidase were measured spectrophotometrically, antioxidant 

activity was measured by Randox kit. Proteins were analyzed by 1D microfluidic 

system Experion, saccharides by HPLC/RI. Except long-term storage conditions also 

influence of some other commonly used technological processes were tested (freezing, 

drying). 

Antioxidant levels differed according to apple sort and colour. A part of phenolics 

formed by flavonoids was in a range of 30 to 60 %. Ascorbate levels detected 

in apples were in range 12 – 51 mg/kg. Freezing exhibited very low influence on 

antioxidant content. Regulated led to about 50% decrease of antioxidants. Changes 

of active substances during long-term storage under controlled atmosphere were 

dependent on apple sort and oxygen concentration. 

Keywords: apples, long-term storage, antioxidants


Posters 

2-P025 Content of Polyphenols and Antiradical 

Activity of bee Pollen 

Katarína Fatrcová-Šramková, Janka Nôžková, Magda Máriássyová, 

Miroslava Kačániová, Eva Dudriková 

Food Research Institute, Centre in Modra, 

Department of Microbiology, Faculty of Biotechnology and Food Industry, SUA Nitra, 

Slovak Republic 

The aim of the study was to measure content of polyphenols and the antiradical 

activity of dried bee pollen (moisture 9–11 %). There were selected following plant 

species Papaver somniferum L., Brassica napus var. rapifera (Moench) Delile, Helianthus 

annuus L. Antiradical activity of various bee pollen samples was determined using 

the free DPPH • radical. Absorbance at 515.6 nm was measured at different time 

intervals using Shimadzu 1601 UV/VIS spectrophotometer (UV-1601, Shimadzu, 

Tokyo, Japan) until the reaction reached a plateau. The absorbance of the 2.2-diphenyl-1-pikrylhydrazyle 

radical (DPPH • ) without an antioxidant (i.e. the control), was 

measured first. The percent of inhibition of the DPPH • radical by the sample was 

then calculated according to the formula: % inhib = [(A C0 - A At )/A C0 ] x 100, where 

A C0 is the absorbance of the control at t = 0 minute, A At is the absorbance of the 

antioxidant at time t minutes, % inhib equals percentage of free DPPH • radicals. 

The polyphenols content in pollen was measured by the Folin-Ciocalteu method. 

The antiradical activity was in the particular samples in range from 48.83 to 86.12 

% (average 71.39 ± 16.45 %). Antiradical activity as determined by the DPPH radical 

scavenging method decreased in the order: Brassica napus var. oleifera (Moench) 

Delile > Papaver somniferum L > Helianthus annuus L. In samples of bee pollen was 

the polyphenol content in the range from 764 do 1378 mg.kg -1 (average 1026.67 ± 

258.31 mg.kg -1 ). Antiradical activity increase in the same order than content of polyphenols. 

This work was supported by Science and Technology Assistance Agency 

under the contract No. APVT-20-026704. 

Keywords: bee pollen, antiradical activity, DPPH, polyphenols 

2.


Posters 

2-P026 Reduction Power, Polyphenols Content and 

Antimicrobial Activity of Honey 

2. 

Katarína Fatrcová-Šramková, Janka Nôžková, Miroslava Kačániová, 

Magda Máriássyová, Eva Dudriková 

Department of Human Nutrition, Faculty of Agrobiology and Food Resources 

University of Veterinary Medicine, Kosice, Slovak Republic 

The aim of the study was to measure content of polyphenols and the reduction 

power of honey from Castanea sativa Mill. and Brassica napus var. rapifera (Moench) 

Delile. Reduction power was evaluated by the method of Prieto. This method is 

established on reduction of Mo (VI) to Mo (V) with an effect of reduction parts in 

the presence of phosphor under formation of green phosphomolybdenum complex. 

Solution absorbance of reducing sample was measured at 705 nm (UV-1601, Shimadzu, 

Tokyo, Japan) toward black experiment (distilled water). Reduction power 

of compounds (RP ) expressed as quantity of ascorbic acid necessary to achieve 

AA 

the same effect in μg.ml-1 was calculated using the equation: RP = (A – 0.0011) 

AA 705 nm 

/ 0.00236. 

The polyphenols content in honey was measured by the Folin-Ciocalteu method. 

The antimicrobial activity of honey against Alternaria infectoria, Scopulariopsis 

brevicaulis, Trychophyton ajelloi and Saccharomyces cerevisiae was studied using the 

agar well diffusion method as well. Reduction power of chestnut honey compounds 

was higher (4083.67 ± 4.50 μg.ml-1 ) than rape honey (3618.33 ± 3.30 μg.ml-1 ). Comparison 

of polyphenols content also refers on higher values in chestnut honey than 

in rape honey (65.33 ± 3.86 mg.kg-1 versus 41.00 ± 2.16 mg.kg-1 ). This work was supported 

by Science and Technology Assistance Agency under the contract No. APVT- 

20-026704. 

Keywords: honey, reduction power, polyphenols


Posters 

2-P027 omparison of pectate hydrolases from parsley 

root cells 

D. Flodrová, E. Stratilová, S. Garajová and J. Omelková 

Faculty of Chemistry, Technical University of Brno 

Purkyňova 118, CZ-61200 Brno 

flodrova@fch.vutbr.cz 

Pectate hydrolases of plants are generally supposed to be bound on primary 

cell wall where the catalysis of homogalacturonan as a side chain of pectin structure 

occures. These enzymes with terminal action pattern (exopolygalacturonases, EC 

3.2.1.67) can be found in each plant species while the production of those with random 

action pattern (polygalacturonases, EC 3.2.1.15) indicates the requirement of 

plant tissue softening. Interestingly, while the function, structure and importance of 

plant polygalacturonases is widely known, the reseach of exopolygalacturonases is 

still on its beginning. References about the strict preference for polymeric substrates 

of these enzymes can be found unlike exopolygalacturonases produced by microorganisms 

which can prefer substrates with lower degree of polymerization, too. 

Five forms of pectate hydrolases purified from parsley roots cells are described 

in this work. Only one of them was strictly bound on the cell wall, enzyme 

with preference for decamer as the substrate. Other three forms, surprisingly with 

the preference for hexagalacturonate, were found in other cell structures. Only one 

typical form of exopolygalacturonase with preference for polymeric substrate was 

identified, partially bound on the cell wall and partially free in cytosole. The action 

pattern of individual forms for oligogalacturonides was evaluated using HPLC. 

Keywords: pectate hydrolases, exopolygalacturonase 

2.


Posters 

2-P028 Mechanism of Bi Bi Reaction of Xyloglucan 

Endotransglycosylase (XET) from Petroselinum 

Crispum Roots 

2. 

S. Garajová, D. Flodrová, V. Farkaš and E. Stratilová 

Institute of Chemistry, Slovak Academy of Sciences 

Dúbravská cesta 9, SK-84538 Bratislava, Slovakia 

chemsong@savba.sk 

Plant xyloglucan endotransglycosylases (XETs, EC 2.4.1.207) react with the 

polysaccharide xyloglucan (XG) by random cleavage of the β-1,4-polyglucose 

backbone of the donor molecule and transfer of the newly created potential 

reducing end to hydroxyl group at C-4 on the non-reducing end of another XG 

molecule or XG-derived oligosaccharide (XGOs) (transglycosylation). There are 

two possible models of Bi Bi reaction mechanism: Pin-Pong and Sequential. In 

the Ping-Pong Bi Bi mechanism, the cleveage and transglycosylation of polyglycan 

chain with newly created reducing end to the acceptor run in two steps, 

where the frist product is released from the stable enzyme-substrate complex 

before the second substrate can bind. In the case of Sequential mechanism as 

an alternative mechanism, the reaction is utilizing practically only one step to 

form a ternary complex of enzyme with both substrates before the cleveage and 

translycosylation. 

The main XET form from parsley (Petroselinum crispum) roots with isoelectric 

point 4.6 showed a broad pH optimum in the region of enzyme pH stability 

4.5-9.0 with one maximum in acidic (5.8) and the second one in alkalic (8.8) 

region. The affinity of this form in dependence on various XGOs acceptors (DP 

8 and 9) was determined as well as the mechanism of Bi Bi reaction in both pHs 

was suggested from the double-reciprocal plots using high-Mr xyloglucan and 

H-labelled XGOs. 

Keyword: xyloglucan endotransglycosylase, Bi Bi reaction


Posters 

2-P029 Multispeciation in Wines by Orthogonal 

Chromatograpy Coupled to UV, Refraction Index 

and ICP-MS Detectors 

T. García-Barrera, J. L. Gómez-Ariza 




Metals can modify the organoleptic properties of wines and the stability, colour, 

and clarity of wines; this is the case of Fe, Cu, Mn, and Zn. Some of them are 

catalysts promoting enzymes; they are also involved in some redox processes in cell 

metabolism. However, a deep knowledge on the different chemical species occurring 

gives greater information about toxicity and action of elements. 

The usual approaches for metal speciation in wines, are based in both atomic 

absorption spectrometry (AAS) and inductively coupled plasma atomic emission 

spectrometry (ICP-AES). The evolution of metals throughout the ripening of grapes 

and vinification processes has also been studied but under our knowledge, the 

SEC/reversed phase (RP)-HPLC on-line coupled to ICP-MS have not been yet performed. 

In this study, the metal complexes characterization in wines has been carried 

out by on-line coupling of SEC-ICP-MS using a Sephadex LH20 column especially 

designed for the separation of low molecular weight compounds (phenols, vitamins, 

anthocyanins and others). After that, fractions of several elements were collected 

and analysed by reverse phase coupled to UV, refraction index detector and 

ICP-MS to get information about complexes of elements with anthocyanins, phenols 

and sugars. Finally, complexes were identified by retention time matching with 

standards of elements or organic compounds and molecular mass spectrometry. 

2.


Posters 

2-P030 Identification of Cu-Metalloproteins in Mus 

Musculus Brain Extracts by Reversed-Phase HPLC 

Coupled with ICP-MS and ESI-Qq-MS 

2. 0 

T. García-Barrera, M. Gónzalez-Fernández, J. López-Barea, C. Pueyo and J. 

L. Gómez-Ariza 


Campus de El Carmen. 21007 Huelva. (SPAIN). 


Orthogonal chromatography systems coupled to ICP-MS and organic mass 

spectrometry has been widely used for metal-biomolecules characterization in biological 

samples connected with in environmental issues. The use of these analytical 

approaches provides very interesting information to deep insight on toxicological 

concern of metals in the environment. The use of heteroatoms can be used as markers 

that simplify the traditional proteomic approaches. 

In previous study, several organs of Mus musculus mice were extracted and 

analyzed by size exclusion chromatography coupled to UV and inductively coupled 

plasma mass spectrometry (SEC-UV-ICP-MS). Some differences were found in the 

molecular mass distribution patterns of elements in the studied organs as in the case 

of a Cu-containig fraction that was only pressent in the brain. This fraction was collected, 

lyophilized and separated by reversed-phase (RP) HPLC following the Cu 

with the ICP-MS. 

In this study, unknown metallobiomolecules have been identificated using 

electrospray coupled to quadrupole time-of-flight (ESI-Qq-TOF) after tryptic digestion. 

2D-PAGE study of the extracts was performed in parallel, for the identification 

of overall proteins, after tryptic digestion of the spots. Comparison of metallomics 

and proteomics results was performed and correlated with Mus musculus genome 

information from data base.


Posters 

2-P031 Analysis of Flavor Compounds in Fruits by GC 

with two Dimensional Detection by FID and MS 

T. García-Barrera, R. Jara Biedma, J. L. Gómez-Ariza 


Campus de El Carmen.21007 Huelva. (SPAIN) 


The fruit juice industry has become one of the world´s major agricultural businesses 

with world trade in fruit juices annually exceeding $ 10 billion [1]. A key 

characteristic of this product is the flavour. Sweet orange (Citrus sinensis) typical aroma 

is attributed to alcohols, hydrocarbons, esters and aldehydes considering solely 

the number of compounds involved. Among these compounds, citral, limonene, 

linalool, α-pinene, ethyl butanoate, acetaldehyde and octanal have been identified 

as most contributing to orange flavour, and can be used in orange juice authentication. 

In addition, some orange juice off-flavours can be developed such as α-terpineol 

that is a well known off-flavour compound present in stored citrus products formed 

from d-limonene or linalool. 

In this work, a method based on liquid-liquid extraction with ethyl acetate and 

later preconcentration has been optimised for the extraction of about twenty flavour 

compounds in orange juice. The identification was based on the retention time and 

the spectra obtained with the MS. Quantification data was obtained with both detectors. 

The levels of the flavour compounds have been used to enhance the quality of 

oranges for industrial juice production and authentication purposes. 

2. 1


Posters 

2-P032 Content of Cadmium in selected Meat Products 

Supplied on the Slovak Marked 

2. 2 

Jozef Golian, Branislav Šiška, Robert Toman, Michal Bošiak 

Fakulty of Biotechnology and Food Sciences, Slovak Agricultural University in Nitra 

Trieda A. Hlinku 2, 949 76 Nitra 

jozef.golian.AF@uniag.sk 

The content of cadmium in selected meat products from Slovak Republic, Czech 

Republic, Germany and Austria was measured. We analysed 360 samples of sausages, 

hams and frankfurters and determined values were compared with Food codex 

of the Slovak Republic. In products from domestic production mean values of cadmium 

were from 0,004 mg.kg -1 (sausages) to 0,247 mg.kg -1 (frankfurters), in products 

from Czech Republic from 0.002 mg.kg -1 (frankfurters) to 0.174 mg.kg -1 (frankfurters), 

in products from Austria from 0.002 mg.kg -1 (hams and frankfurters) to 0,147 

mg.kg -1 (frankfurters) and in products from Germany from 0,002 mg.kg -1 (sausages) 

to 0.212 mg.kg -1 (frankfurters). Excess of the highest permissible value for cadmium 

was detected in 17 samples from Slovak Republic, in 15 meat samples from Czech 

Republic, in 14 samples from Austria and in 11 samples from Germany. 

Keywords: cadmium, meat products


Posters 

2-P033 Chiral Speciation of Iodine in Thyroid Hormones 

by Using HPLC-MS/MS and HPLC-ICP-MS 

J. L. Gómez-Ariza, E. Gómez de las Heras, T. García-Barrera 




Iodine is an essential element for humans and animals generally included into 

thyroid hormones. The deficiency of this element can produce mental disease and 

poor physical development in children and goiter in adults. For this reason new 

diets that include iodine in supplements are becoming recommended. The most 

important bioactive iodine species are: iodo-tyrosine (MIT), diiodo-tyrosine (DIT), 

diiodo-thyronine (T2), triiodo-thyronine (T3), thyroxine (T4). These species have 

chiral centres, and the different enantiomers forms have shown differential biological 

effectiveness. 

Iodine speciation is frequently based on the coupling of HPLC with UV detection, 

especially for pharmaceutical formulations [1]. However, the sensitivity obtained 

with this detector is low and the species identification is not unequivocal. 

For this reason, other authors have proposed the use of HPLC-MS, especially for 

biological and food matrices in which iodine concentration is low and critical. 

In the present work, a comprehensive analytical approach based on the parallel 

use of HPLC-MS/MS and HPLC-ICP-MS has been optimized for iodine speciation 

and applied to algae used as potential source for iodine supplement in humans. 

2.


Posters 

2-P034 Development of a rapid arsenic Speciation 

Procedure for Marine Origin Food based on 

Ultrasound Probe Extraction 

and HPLC-UV-HG-AFS 

2. 

J. L. Gómez-Ariza, M. Contreras-Acuña, T. García-Barrera 



ariza@uhu.es 

Arsenic is widely distributed in environmental and biological systems. Total 

As content does not give usually enough information about the environmental and 

health concern of this element, and a deep knowledge on the different chemical 

arsenic-species occurring in environmental components and biological pathways 

gives greater information about As toxicity and action. It is generally accepted that 

the toxicity of arsenic is greater for inorganic species that for organic species. The 

natural occurrence or anthropogenic origin (e.g. pesticides, fertilizers) of As in water 

and soils, and the food chain, result in exposure of humans to different As species, a 

list that includes more than twenty species of natural origin. 

Different analytical approaches have been propose for arsenic speciation, 

mainly based on the coupling of HPLC with ICP-MS or atomic fluorescence (AFS) 

detectors, this latter preceded by arsenic species derivatization by hydrides generation. 

However, the critical step in arsenic speciation is species extraction from complex 

matrices, especially those from marine origin, such as bivalves or fish that are 

latterly used as human food. 

In the present study the use of ultrasound probe for fast and efficient extraction 

of arsenic species from marine origin food has been evaluated in comparison 

with well established methods that use other non focalised energy such as MW and 

ultrasound bath. Arsenic species characterization was traced by using HPLC-UV- 

HG-AFS.


Posters 

2-P035 Comparison of Proteome and Metabolome 

Changes in Stressed Yeast Strains Rhodotorula 

Glutinis and Rhodotorula Rubra 

Andrea Halienová, Ivana Márová, Zbyněk Zdráhal, Hana Konečná, 

Martina Čarnecká, Kateřina Pařilová, Emília Breierová 



In this work a proteome and metabolome changes in two strain of red yeasts – 

Rhodotorula glutinis and Rhodotorula rubra - were studied. Both strains were stressed 

by high and/or low concentration of salt and hydrogen peroxide. Proteome changes 

were analyzed using 2D gel electrophoresis. First, a complex procedure for red yeast 

proteins isolation excluding enzyme lysis was necessary to optimize. Lyophilized 

cell cultures were prepared as starting material and several precipitation and extraction 

steps were used for protein purification. Yeast proteome is quite complex, so, 

interpretation of 2D results is very difficult. After 2D-GE selected individual spots 

were analyzed in detail using LC/MS/MS. According to proteomic analysis it seems, 

that individual strains differ in their response to oxidative and osmotic stress. In 

both strains stressed by both salt and peroxide expression of some similar dominant 

proteins was influenced. Some spots were analyzed and proteins were identified 

according to databases. Further analysis is needed for detailed characterization of 

individual spots. Metabolome analysis confirmed, that R.glutinis is better producer 

of beta-carotene than R.rubra. 

Keywords: proteomics, 2D-electrophoresis, beta-carotene, Rhodotorula 

2.


Posters 

2-P036 (1→3)(1→4)-β-D-Glucan: Variability and Factors 

Affecting Its Content in Oat Grain 

2. 

Michaela Havrlentová, Ján Kraic, Milan Čertík 

Slovak Agricultural Research Centre – Research Institute of Plant Production Piešťany 

Bratislavská 122, 921 68 Piešťany, Slovak Republic, 

havrlentova@vurv.sk 

β-D-glucan, an important cell wall polysaccharide of the Poales, plays a significant 

role in human diet due to its beneficial health effects. High variation of β-Dglucan 

content was observed in 99 oat genotypes and some features influencing the 

biosynthetic mechanism of studied metabolite were disclosed. 

Naked genotypes dispose of higher levels (4.48 % on average) of this metabolite 

in comparison with the glumoses one, where the mean content was 3.17 %. Genotypes 

with black colour of the glumes account significantly lower standard deviation 

and variation coefficients in the content of β-D-glucan, what indicates markedly 

stable biosynthetic mechanism of studied metabolite. 

Therefore our next efforts will be target to the gene analysis responsible for the 

β-D-glucan biosynthesis. Genotypes SV-5, PS-106, Neon, Šampionka, Arnold, and 

Adam with the highest level (>4.50 %) observed can be used in breeding programs 

and seed processing in food industry as a convenient raw material for production 

of functional foods. 

Key words: β-D-glucan, oat


Posters 

2-P037 Extraction and Determination of Flavonoids in 

Plant Materials 

Barbora Hohnová , Pavel Karásek, Milena Vespalcová 

Faculty of Chemistry, Brno University of Technology, 


hohnova@fch.vutbr.cz 

Pressurized fluid extraction (PFE) followed by reversed phase high-performance 

liquid chromatography with UV-visible detection has been utilized for the 

determination of a group of flavonoids with related structures (quercetin, luteolin, 

apigenin and kaempferol) in plant materials. The effects of solvent composition, 

temperature and extraction time on PFE efficiency have been studied. The compounds 

were monitored and quantified at 360 nm. 

Keywords: PFE, flavonoids 

2.


Posters 

2-P038 Monitoring of Pesticide Residues in Commodities 

Used for Baby Food Production 

2. 

Jiří Honzíček, Jana Hajšlová, Vladimír Kocourek 

Institute of Chemical Technology, Department of Food Chemistry and Analysis 

Technická 3, 166 28, Prague 6, Czech Republic 

jiri.honzicek@vscht.cz 

Currently, more than 800 pesticide active ingredients in a wide range of commercial 

products are registered for use in agriculture to meet food supply demands. 

However, under certain circumstances, residues of active ingredients occur in 

treated crops at the time of harvest, even when they are applied in accordance with 

Good Agriculture Practise (GAP). The presence of those residues causes potential 

health risk for consumers resulting from acute and/or chronic dietary exposure. 

In this study, we collaborated with one of the most important producer of baby 

food in the Czech Republic by analysing the entrance as well as intermediate commodities 

for production of baby food. For determination of pesticide residues two 

complementary analytical strategies were used: gas chromatography–mass spectrometry 

(GC–MS) and liquid chromatography–tandem mass spectrometry (LC–MS/ 

MS). From the 122 samples examined, 76.8% represented positive ones, i.e. concentrations 

of residues were higher than limits of quantification (LOQs) of particular 

multiresidual methods. The most frequently identified residues were: acetamiprid, 

carbaryl, carbendazim,cyprodinyl, DMST, dodine, fenbuconazole, fenhexamid, 

chlorpyrifos, imidacloprid,kresoxim-methyl, methamidophos, myclobutanil, parathion, 

phosalone, pirimicarb,pirimphos-methyl, propargite, pyridaben, pyrimethanil, 

tetraconazole, thiacloprid, and athiophanate-methyl. 

Keywords: pesticide residues, baby food, LC–MS/MS; GC–MS


Posters 

2-P039 Trichosporon Cutaneum: Cell Adhesion on 

Cellophane Surface 

J. Hrdinová, T. Krulikovská,V. Jirků, O. Schreiberová, A. Čejková, J. Masák 

Institute of Chemical Technology Prague , Faculty of Food and Biochemical Technology, 

Department of Fermentation Chemistry and Bioengineering, 

Technická 5, 166 28 Praha 6 – Dejvice, Czech Republic 

jitka.hrdinova@vscht.cz 

Cell adhesion of microorganisms on insoluble polymeric substrates is one of 

the first (necessary) steps in their biodegradation. 

In this connection, the early and later phases of the yeast Trichosporon cutaneum 

biofilm formation on the cellophane surface were investigated and described under 

various physiochemical conditions. 

The colonization of a cellulosic material was observed using cellophane as a 

sole source of carbon. The effect of other nutrients profile, shear stress, cell density, 

growth phase and pH was studied in Flow Cell FC 81. 

Keywords: yeast adhesion, cellophane 

2.


Posters 

2-P040 Seasonal Changes of Rubisco Activity and its 

Content in Norway Spruce Exposed to Ambient 

and Elevated CO 2 Concentrations 

2. 0 

Miroslav Hrstka, Lucie Zachová, Otmar Urban 



hrstka@fch.vutbr.cz 

Rubisco initial and total activities as well as Rubisco content in Norway spruce 

(Picea abies) grown in ambient (A = 360 µmol CO 2 mol -1 ) and elevated (E = 700 µmol 

CO 2 mol -1 ) concentrations of carbon dioxide were measured. Both total and initial 

activities decreased during the growing season but a diminution of the activities in 

E was slower than in A. Rubisco content in A decreased during the whole growing 

season. Rubisco content in E decreased rapidly in the first half of the growing season 

(about 75 %) and increased in the second half of the growing season. We concluded 

that there is a downregulation of Rubisco content in the Norway spruce exposed to 

E in the first half of the season. 

Key words: Rubisco activity, Rubisco content, Norway spruce, downregulation.


Posters 

2-P041 Enzyme Degradation of LIPIDS and its 

Application in lipid –Containing Wastewater 

Kateřina Illková, Jiřina Omelková, Bohumila Vlčková 



xcillkova@fch.vutbr.cz, 

The ability of five enzymatic preparations to degrade the lipids in wastewater 

was studied. The aim of this study was to evaluate this preparations and to determine 

the preparation with the best efficiency. Based on the experimental results the 

optimal conditions for their application will be suggested. In all preparations the 

lipolytic activity was assessed using spectrophotometric method. Simultaneously, 

the temperature and time dependence was studied . 

Keywords: lipid degradation, lipolytic activity, wastewater 

2. 1


Posters 

2-P042 Gas Chromatography-Mass spectrometry 

Characterization of Sea Buckthorn 

2. 2 

Andreea Iordache, Radu Minea, Monica Culea, Camelia Lehene 

Babeş-Bolyai University, Biomedical Physics Department, 

1 Kogălniceanu St., 400084 Cluj-Napoca, 

Romania 

Quantitative analysis of polyunsaturated and polysaturated fatty acids in sea 

buckthorn (hippophae rhamnoides L) is presented. The internal standard used was 

C11:1 for fatty acid methyl esters determination. The extraction procedure was performed 

in dichloroethane and by maceration. The extraction was followed by methylation 

of fatty acids with methanol HCl 3M, at 100 o C, 30 min and gas chromatographic 

and gas chromatography- mass spectrometric analysis. A Rtx-5MS capillary 

column, 30mx0.32mm, 0.25μm film thickness, in a temperature program: 50 o C (2 

min) then 8 o C per minute to 310 o C (8min) was used. The method was applied for 

testing fatty acid changes after the treatment of sea buckthorn oil at different kGy 

doses.


Posters 

2-P043 Rapid Authentification of Natural Juices 

by GC-MS 




Romania 

Abstract. A GC-MS analytical method is described for some natural juices analysis. 

The fingerprint of sterols was used to characterize the natural juice. A rapid 

liquid-liquid extraction method was used. The sterols were separated on a Rtx-5MS 

capillary column, 15mx0.25mm, 0.25μm film thickness, in a temperature program 

from 50 o C for 1 min, then ramped at 15 o C/min to 300 o C and held for 15 min. Identification 

of sterols and their patterns were used for juice characterization. The sterol 

profile is a useful approach for confirming the presence of juices of orange, grapefruit, 

pineapple and passion fruit in compounded beverages and for detecting of 

adulteration of fruit juices. 

Keywords: gas chromatography, mass spectrometry, mass spectra, 

authentification 

2.


Posters 

2-P044 Comparative Extraction Methods of some 

Biologic Active Compounds in Herbs 

2. 




Romania 

Different extraction methods have been compared for the recoveries study by 

using a synthetic mixture of some compounds found in plants. The extraction was 

compared at the same variables in the optimization process: amount of samples, 

extracting solvent volume, extraction temperature, extraction time. A liquid –liquid 

extraction method (LLE) was compared with two different solid-phase extraction 

method (SPE), a microwave one (MWE) and ultrasonic extraction one (USE). Good 

recovery values for a standard mixture were obtained. Precision of extraction methods 

gave relative standard deviation lower than 3%. The extracts were analyzed by 

GC and GC/MS. A HP-1 capillary column, 30m x 0.32 nm, 0.25μm film thick-ness, in 

a temperature program from 50 0 C, kept 2 minutes, then increased to 250 0 C, with a 

rate of 8 0 C per minute was used. By using an appropriate mixture of solvents, good 

recovery mean values were obtained by LLE (96%) comparable with the recoveries 

of other extraction methods as SPE (86%), MWE (103%) and USE (101%). The study 

was applied for fingerprint chromatograms to characterize the flavors extracted 

from herb plants of different sources. 

Key words: GC, GC/MS; liquid-liquid extraction (LLE); solid phase extraction 

(SPE); microwave extraction (MWE); ultrasonic extraction (USE).


Posters 

2-P045 Bioremediation of Botton Sediments using 

Bacillus Megaterium and Bacillus Cereus 

Katarina Jablonovska, Iveta Styriakova 

Department of Biotechnology, Institute of Geotechnics of the Slovac Academy of Science 

Watsonova 45, 043 53 Kosice, Slovak republic 

jablonov@saske.sk 

A water reservoir Ružín I. lies in an area, which several centuries is known for 

mining and metallurgical activities. Bottom sediments are contaminated with heavy 

metals, in the concrete with Hg, Cu, Mn, Zn, Ni and Cd, which were wash away into 

the water reservoir from locations of former mining activities. Enhanced amounts of 

heavy metals preclude of direct utilization of sediments in agricultural and building 

industry and also in ground shaping. 

The availing of bioleaching techniques on mobilization of heavy metals from 

sediments seems to be an appropriate manner of retreatment. In this attempt we 

used beside indigenous microflora bacterial species Bacillus megaterium and Bacillus 

cereus too. These bacterial species were izolate from the soil matrix of sampling place 

Hornád-inlet. As a result of the sediment bioleaching in laboratory conditions was a 

reduced amount of Ni (about 9%), Cd (about 6,5%) and Zn (about 17%). 

Keywords: sediment, Bacillus sp., bioleaching 

2.


Posters 

2-P046 Biosorbents Preparation for Heavy Metals 

Removing from Waters 

2. 

Jana Jenčárová, Alena Luptáková 



jencarova@saske.sk 

Presence of heavy metals in waters is relatively often indicator of their pollution. 

Usage metals in various sectors of industry cause increased concentration 

of heavy metals in waters. Potential ecological or public health risk, which metals 

represent, depends largely upon the form in which they occur. 

At the present time are several techniques to remove heavy metals from waters. 

One of the possibilities is usage of microorganism, concrete sulphate-reducing bacteria 

(SRB). Iron sulphide precipitates produced by SRB as their metabolic products 

are the excellent sorbents for a wide range of heavy metals ions. 

The aim of this work is to study the possibility of using SRB (Desulfovibrio sp.) 

from source Gajdovka (Košice) for the preparation of iron sulphide sorbents. Experiments 

were realized in the batch reactor under anaerobic conditions at 30°C. Originate 

precipitates were removed from liquid phase by filtration and consequently 

used for the sorption experiments. 

Keywords: biosorbents, sulphate-reducing bacteria, heavy metals


Posters 

2-P047 Genotype Variability of Fatty Acids in Cereal 

Grains 

Dalibor Ješko, Milan Čertík 

Slovak Agricultural Research Centre – Research Institute of Plant Production Piešťany, 

Bratislavská 122, 921 68 Piešťany, Slovak Republic, 

email: jeskod@vurv.sk 

Cereals are important part of human and animal nutrition. Because lipids are 

essential compounds of cereal grains, this study was focused on genotype variability 

of fatty acid composition in following cereal grains: oat (85 varieties), millet (29 

varieties), amaranth (20 varieties) and buckwheat (10 varieties). Lipid amounts in 

investigated cereals varied between 1.5 to 8.2% and palmitic, oleic and linoleic acids 

are major fatty acids. Correlation between levels of oleic and linoleic acids indicated 

that activity of ∆ 12 − desaturase might be controlled genotypically. Variations in individual 

fatty acid levels also demonstrated deviation in biosynthesis stability of 

these compounds in selected cereal genotypes. Fatty acid composition together with 

calculated fatty acid parameters offers not only valuable taxonomic and physiologic 

information for classification of various cereal varieties, but also may be used by 

farmers and producers for aimed applications of cereals from the view of desired 

lipid attributes. 

Keywords: cereal grains, lipids, fatty acids 

2.


Posters 

2-P048 Preliminary Simultaneous Studies on the 

Antifungal Activity and Chemical Composition 

of the Hexane Extract of the Brazilian 

Chenopodium Ambrosioides L. 

2. 

Gulab Newandram Jham, Carolina Marangon Jardim, 

Onkar Dev Dhingra and Marcelo Moreira Freire 

Fedral Universsity of Virosa 

Department of Chemistry, Minas Gerais, 36571-000 Brazil, 

gulab@ufv.br 

Presently fungi that invade stored agricultural commodities are managed by 

synthetic compounds known to be harmful to man and environment and alternative 

control methods are urgently needed. The potential of plant extracts has been little 

studied. The antifungal activity of the hexane extract of the Brazilian epazote (Chenopodium 

ambrosioides L.) was evaluated by the poison food assay at concentrations 

of 0.1, 0.2 and 0.3% using four post-harvest deteriorating fungi (Aspergillus flavus, 

A. glaucus, A. niger and A. ochraceous). Depending upon the fungus, an inhibition of 

26.2-100% was obtained. The following six compounds were identified in the crude 

extract by Kováts retention indexes (RIs) using GC (gas chromatography) and GC- 

MS (gas chromatography combined with mass spectrometry): α-terpinene (11.2), 

p-cymene (6.0), benzyl alcohol (0.4), (Z)-ascaridole (54.0), carvacrol (2.3) and (E)ascaridole 

(17.3). 

Keywords: Chenopodium ambrosioides; Essential oil


Posters 

2-P049 Evaluation of antioxidant and antimicrobial 

effects of natural honeys 

Miroslava Kačániová, Martina Fikselová, Soňa Felšöciová, 

Vladimíra Kňazovická, Katarína Fatrcová-Šrámková, Janka Nôžková 

Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, 

Tr. A.Hlinku 2, Nitra, 949 76 Slovakia, 

miroslava.kacaniova@uniag.sk 

The antioxidant activity and antimicrobial properties of 9 natural honeys were 

tested in this work. The antioxidant activity in term of their antiradical power was 

assessed by DPPH radical scavenging assay. Honey samples (8) were collected from 

different places of Slovakia and one sample originated from Rodos. 

Honeys were derived from different plant species of acacia (2 samples), rape (1 

sample), thyme (1 sample) and 5 samples were of floral origin. Samples were dissolved 

in ethanol (90 %). Results were expressed as % inhibition of DPPH radical. 

The best results were found at thyme honey from Rodos (11.84 %) and floral honeys 

from different places of Lučenec (10.09 %, 10.19 %). The lowest antiradical activity 

was determined at Acacia honey (7.615 %). 

The inhibitory action of honey samples was tested against Penicillium griseofulvum, 

P. raistrickii, P. verrucosum, P. expansum and P. crustosum by the agar well diffusion 

method. Honey samples were tested after dilution to 50, 25 and 10 % (by mass 

per volume). The solutions containing 10 % (by mass per volume) of honey did not 

show any effect on the growth of fungi; some of honey samples had no inhibitory 

activity on any of the concentrations used. 

Keywords: antioxidants, antiradical activity, honey, antimicrobial activity, 

Penicillium genus 

2.


Posters 

2-P050 Combined Technique LC/MS in Analyses of 

Antioxidant Compounds 

2. 0 

Radka Kočí, Marie Trčková, Lukáš Müller, Ivana Márová 

Department of Food Chemistry and Biotechnology, Faculty of Chemistry, 



koci-r@fch.vutbr.cz 

Antioxidants constitute a large group of plant and microbial secondary metabolites 

(for exemple carotenoids, phenolics, oligopeptides, saccharidic derivates etc.) 

with major interest because of their anti-inflammatory effects and of their potential 

anti-atherogenic properities. Many foods are good sources of antioxidants: vegetables, 

legumes, whole-grain cereals, tea extracts, honey etc. The best source of these 

physiologically active compounds with rapid effects on human health is represented 

by some sorts of fruit. Especially citrus fruit contain high-levels of phenolics and/or 

ascorbic acids, while apples are mostly consuming fruit with moderate amount of 

antioxidants. 

Presented work was focused on characterisation of several substances (phenolics, 

carotenoids) with antioxidant activity containned in select biologic materials 

and on their distribution in individual sample fractions (juice, pulp, peel). Antioxidant 

compounds were isolated i) by ethylacetate extraction and/or by acetone 

extraction; ii) using SPE method. Analyses were realised with combined technique 

HPLC/MS on-line and/or off-line, eventually compared with UV-VIS detection. 

Keywords: phenolics, carotenoids, LC-ESI/MS, SPE


Posters 

2-P051 Influence of Chemical Treatment on Fusarium 

Toxins in Barley Harvested 2005-2007 

Jana Kohoutková, Kateřina Lancová, Alexandra Krplová, Ondřej Lacina, 

Jana Hajšlová, Jana Marková, Jaroslava Ehrenbergerová 

Institute of Chemical Technology, Pratur 

Department of food chemistry and analysis 

Technická 5, 166 28 Prague 6, Czech Republic, 

Jana.kohoutkova@vscht.cz 

Mycotoxins are toxic secondary metabolites produced by certain toxigenic microscopic 

fungi in various crops used as raw materials for the production of foodstuffs 

and fodder. Fusarium toxins have been shown to cause a variety of toxic effects 

(both acute and chronic) in humans and animals (vomiting, dermal irritation, 

haemorragic lesions, weight reduction, etc.). 

The main objective of this study was to investigate the influence of key agricultural 

practices (resistance of barley cultivars, chemical treatment use, pre-crop 

and different growing locality) on Fusarium mycotoxins in barley. Field trials were 

realized in cooperation with the Agricultural Research Institute Kroměříž (harvest 

2005, 2006 and 2007). 

For the determination of the most important Fusarium mycotoxins (deoxynivalenol, 

HT-2 toxin, T-2 toxin, zearalenone, nivalenol, 15-acetyldeoxynivalenol, fusarenon-X 

and 3-acetyldeoxynivalenol), high-performance liquid chromatography 

coupled with tandem mass spectrometry (LC-MS/MS) was used. 

The trueness of the generated data was successfully demonstrated in proficiency 

tests (Food Analysis Performance Assessment Scheme, FAPAS ® ) organized by 

Central Science Laboratory (CSL, York, UK). 

Keywords: mycotoxins, DON, Barley 

2. 1


Posters 

2-P052 Monitoring of Acrylamide in Foods : “The 

Project on Dietary Exposure of the Czech 

Population to Selected Chemical Substances” 

2. 2 

J. Kolářová, S. Saláková, I. Řehůřková, J. Ruprich 

National Institute of Public Health – Centre for Hygiene of Food Chains, 

Palackého 3a, 612 42 Brno, Czech Republic, 

kolarova@chpr.szu.cz 

Acrylamide is an industrial chemical used as a chemical intermediate in the 

production of polyacrylamides, which are used as flocculants for clarifying drinking 

water and industrial applications. Experimental studies with acrylamide in animals 

have shown reproductive, genotoxic and carcinogenic properties. The Swedish 

National Food Authority reported on elevated levels of acrylamide in heat-treated 

potato products and baked goods in April 2002. 

Since 2004, content of acrylamide has been monitored within the framework 

of “The Project on Dietary Exposure of the Czech Population to Selected Chemical 

Substances” carried out by the Centre for the Hygiene of Food Chains in Brno with 

the objective to describe the dietary exposure of the Czech population to chemical 

substances. 

The monitoring of acrylamide is performed in the foods starch – containing. 

The samples undergo the culinary treatment so that they are analysed in the same 

state as they are consumed. 

The analytical procedure consists of isolation acrylamide from a matrix and of 

brom derivatisation of acrylamide with consecutive analyse using GC-MS method. 

The highest content of acrylamide was found in the potato crisps (271–3817 

μg/kg), french fries (až 705 μg/kg) and spice (147–1076 μg/kg). 

Keywords: acrylamide, monitoring, foods, GC-MS


Posters 

2-P053 Trans Fatty Acids in Diet of the Czech 

Population During year 2007 

J. Kolářová, J. Hradilová, I. Řehůřková, J. Ruprich, S. Saláková, 

D. Pečinková 

National Institute of Public Health, Centre for the Hygiene of Food Chains, 

Palackého 3a, 612 42 Brno, Czech Republic, 

hradilova@chpr.szu.cz 

Trans fatty acids (TFA) are formed in technological and microbiological processes 

by isomerization of cis double bonds to trans double bonds. Small amounts 

of trans fat are produced in the rumen of ruminants and found in dairy and beef 

fat. Trans fat are predominately produced through a process called partial hydrogenation. 

TFA increase the risk of coronary heart disease and of other chronic health 

problem (cancer, diabetes, obesity, interfility). 

The National Institute of Public Health in Prague, Centre for the Hygiene of 

Food Chains in Brno is involved in “The Project on Dietary Exposure to Selected 

Chemical Substances”. The objective of the project is to describe the dietary exposure 

of the population of the Czech Republic to chemical substances. In the framework 

of this project fatty acids have been monitored since 2005. Fatty acids were 

determined in food samples in the full range consumed by the Czech population. 

The analytical procedure consists of the three steps – extraction of the triacylglycerols 

from the matrix, saponification and ester interchange and finally analysis 

using the GC – FID method. 

The highest concentrations of TFA were found in gingerbread, wafers and biscuits. 

Very low concentration of TFA were found in pork liver, sea fish, flour and 

cocoa. 

Key words: trans fatty acids, foodstuffs, GC – FID 

2.


Posters 

2-P054 The Monitoring of Dietary Exposure of Czech 

Population to Persistent Organic Pollutants 

2. 

Jana Kolářová, Sylva Saláková, Irena Řehůřková, Jiří Ruprich 

National Institute of Public Health in Prague, Centre for Hygiene of Food Chains 

Palackého 3a, 612 42 Brno, Czech Republic 

salakova@chpr.szu.cz 

Persistent organic pollutants ( POPs) are organic compounds that are resistant 

to environmental degradation through chemical, biological and photolytic processes. 

They are highly persistent, lipophilic and bioaccumulative industrial contaminants. 

POPs accumulate in fat of organisms. Some of them are classified as possible 

human carcinogens. Therefore, for many years, POPs are controlled substances as 

contaminants in our environment. 

Within The Project on Dietary Exposure to Selected Chemical Substances 37 

POPs was monitored. 

POPs were determined in food samples of the so-called food basked of the 

Czech population. 

The food samples are first culinary treated so that they could be analysed in 

the same stage as they are consumed. The result of the preanalytical treatment is a 

homogenous sample, which is then analysed. The first step of the sample preparation 

is an extraction. After extraction the samples are cleaned by usage the GPC 

method. Last step of sample preparation is the column chromatography. Cleaned 

samples are then analysed by GC chromatography on two columns system with 

ECD detectors. 

We analysed 110 samples of foodstuffs per one year. The highest content of 

POPs was observed in meat products, butter and milk products and fish. The nonfat 

samples are logically less contamined group for this analytes. 

Keywords: persistent organic pollutants , gas chromatography


Posters 

2-P055 Occurrence of Fusarium Mycotoxins and their 

Conjugated Forms in Cereal-based Foods 

Marta Kostelanská, Jana Hajšlová, Milena Zachariášová, 

Alexandra Krplová, Jan Poustka, Jana Kohoutková 

Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, 



marta.kostelanska@vscht.cz 

Trichothecene mycotoxins, toxic secondary metabolites produced by a fungi 

species of the genus Fusarium, can adversely affect animal and human health. Cereals 

are frequently infected by these molds in the field world-wide. One of the most 

abundant fusariotoxin is deoxynivalenol (DON). Recently, it has been shown, that 

in addition to its native form, DON can be also found in plants conjugated to glucose. 

In presence, great scientific effort is spent to monitor both above mentioned 

compounds. 

With regard to high consuption of cereal-based products, the control of mycotoxins 

levels in this type of foodstuffs is a food safety issue of high importance. It 

is essential to understand both the primary cereals contamination and influence of 

processing on the mycotoxins levels in final products. 

In this presentation, the influence of technological process on both absolute 

levels and mutual ratios of DON and D3G in bread and beer will be presented and 

dynamic changes of mycotoxins concentrations during each technological step will 

be disscused. Moreover, the validation of HPLC-MS/MS method used to obtain experimental 

data will be described. 

Keywords: mycotoxins, bread, beer, HPLC-MS/MS 

2.


Posters 

2-P056 Lignans in Flaxseeds – LC-MS/MS Determination 

2. 

Anna Krajcova, Vera Schulzova, Jana Hajslova, Zuzana Zakova, 

Marie Bjelkova 


Faculty of Food and Biochemical Technology, 

ICT Pratur Technicka 5, 166 28 Prague 6 

anna.krajcova@vscht.cz 

Lignans are phytoestrogens widely distributed in the plant kingdom. They 

have been suggested to induce a wide range of biological effects, such as antitumor, 

both estrogenic and antiestrogenic activities etc. Antioxidant potential has also been 

documented for many representatives of this group. One of the reachest dietary 

source of lignans are flaxseeds, with glycosides of secoisolariciresinol (SECO) and 

matairesinol (MAT) as the major components. 

In this study, a quantitative method for the determination of plant lignans 

SECO and MAT in flaxseed is presented. The analytical procedure involves enzymatic 

hydrolysis (β-glucuronidase/sulfatase) of defatted milled flaxseeds, cyclohexane:ethylacetate 

extraction, and, subsequently separation and detection employing 

LC-MS/MS. Wide range of flaxseed samples was examined to assess the influence 

of various factors such as variety, way of farming etc. on the content of SECO and 

MAT. 

Keywords: flaxseed, lignan


Posters 

2-P057 The Occurence of PhthalicAcid Esters in 

Selected Feedstuffs 

Lenka Krátká, Alžbeta Jarošová, Jana Malyszová, Vlasta Stancová, 

Jiří Harazim 

Mendelova zemědělská a lesnická univerzita Brno 

Zemědělská 1, 613 00, Brno 

krtaska@seznam.cz 

Samples of feedstuffs, feed additives and premixes for livestock were collected 

by ÚKZUZ (Central Agricultural Control and Testing Institute). They were collected 

in years 2005 and 2006. Feeds were analysed for phthalic acid esters – di-n-butyl phthalat 

(DBP) and di-2-ethylhexyl phthalat (DEHP). Total phthalates (ΣDBP+DEHP) 

measured in 2005 reached levels from 2,03 mg.kg -1 (corn) to 131,42 mg.kg -1 (soybean 

oil). High levels were contained in animal fat (58,87 mg.kg -1 ) a feed additive Alimet-methionine 

(38,36 mg.kg -1 ). Feedstuffs were richer for PAEs. In 2006, levels of 

ΣDBP+DEHP ranged between 0,12 mg.kg -1 (Vitamin A) and 4,52 mg.kg -1 (Alimetmetionine), 

feedstuffs contained higher levels of PAEs, from 0,04 mg.kg -1 (wheat) to 

23,87 mg.kg -1 ΣDBP+DEHP (rapeseed oil). The highest levels were determinated in 

raw materials with fat matrix, so the lipophilic character of PAEs was confirmed. 

Samples of oils A, B, C were collected in 2007. Oils B and C were stored in plastic 

barrel. Levels of ΣDBP+DEHP reached in A from 5,14 mg.kg -1. to 10,13 mg.kg -1 . . Total 

phthalates in oils C were contained in levels from 31,12 mg.kg -1. to 61,55 mg.kg -1 .. 

Study confirmed, that PAEs can easily migrate from platics to fat. 

This study was carried out within the scope of project NAZV 234/2202/MZ260041, 

supported by the Department of Agriculture, Res. & Dev., Czech Republic.. 

Keywords: phthalates, feedstuffs 

2.


Posters 

2-P058 New Approach in Analysis of Fusarium 

Mycotoxins and their “Masked” Forms: 

Immunoaffinity Column Clean-up 

2. 

A. Krplova, A. Kostelanska, M. Zachariasova, J. Hajslova and 

J. Kohoutkova 

Institute of Chemical Technology Prague, 


alexandra .krplova@vscht.cz 

Deoxynivalenol-3-glucoside (D3G) has been recently found in high amounts in 

beer and other fermented cereal-based products. Presence of “masked” mycotoxins 

was detected in unprocessed cereals as well. Analysis of mycotoxins in single run 

is complicated due to differences in polarity between parent deoxynivalenol (DON) 

and its glycosilated derivates. 

Immunoaffinity clean up application improved performance characteriristics 

of liquid chromatography with tandem mass spectrometry (LC-MS/MS). Commerical 

columns antibodies cross reactivity enables antibodies binding with “masked” 

mycotoxins, significantly decreases matrix interferences during detection and so 

improves analysis performance. Immunoaffinity columns specific for several fusariotoxins 

(DON, HT-2 and T-2 toxin, zearalenon) from various suppliers were tested. 

Differences in antibodies function were documented. 

Keywords: deoxynivalenol, deoxynivalenol-3-glucoside, 

immunoaffinity columns, Fusarium


Posters 

2-P059 The effect of cell adhesion on polutant 

biodegradation 

Tereza Krulikovská , Olga Schreiberová, Jitka Hrdinová 

Jan Masák, Alena Čejková, Vladimír Jirků 


Department of Fermentation Chemistry and Bioengineering 

Technicka 5, 166 28 Prague, Czech Republic 

tereza.krulikovska@vscht.cz 

The unicellular microorganisms naturally form multicellular communities 

which differentiate into specialize cells that bring a benefit to whole population. 

Predominant multicultural form of bacteria is called biofilm. Bacterial biofilms are 

generally described as surface associated community consisting of microcolonies 

surrounded by a matrix of exopolymers with varied composition. The characteristics 

of biofilm which are in focus of present research are the ability to withstand and 

degrade high concentration of various toxic substances. 

Gram-positive Rhodococcus erythropolis was chosen for our experiments. Bacteria 

were cultivated in mineral medium under different cultivation conditions. A several 

toxic substances were used as a sole carbon source (e.g.: phenol, aniline, catechol). 

The ability of bacterial cell to adhere on different polymer materials was tested by 

several methods: colorimetric method (staining and distaining of sessile cells with 

crystal violet), microscopic observation (fluorescent and confocal microscopy). The 

biodegradation of various pollutants by cells adhered to different polymer carriers 

were determined by HPLC. 

Keywords: polutant biodegradation, biofilm, Rhodococcus erythropolis 

2.


Posters 

2-P060 Categorization of Olive Oils 

2. 0 

Dáša Kružlicová, Ján Mocák, Ernst Lankmayr 


University of Ss. Cyril & Methodius, SK-91701 Trnava, Slovakia 


Olive oil is an important food component, which enjoys increasing popularity 

in many countries not only due to its delicate taste but also because of its nutrition 

value. Depending on regional conditions a variety of oils are produced in different 

qualities. 

193 olive oil samples of five different olive oil types determined for consumer 

market have been characterized by the UV-Vis spectra. Absorbances at 2001 wavelengths 

were measured in the range of 230-700 nm. Chemical characteristics - acidity, 

peroxide value, and the oxidation indicators K232 a K237 were also measured. 

The sensory evaluation of the investigated oil samples was made in a ten-point scale 

by the experienced panel of experts. This panel rated several properties of the oils, 

especially smell, taste and visual character. In addition, the presence of water was 

thoroughly checked since a colloidal mixture of water and residual proteins remarkably 

affects (diminishes) the oil quality. Three basic classes of oils, expressing the oil 

quality, were created: highest quality - with the score 7-9 points, medium quality 

(4-6 points) and low quality (1-3 points). 

Classification of the examined oils was achieved by chemometrical data processing, 

by which prediction of two oil characteristics are possible: (a) the oil origin, (b) 

the oil quality. Four methods of discriminant analysis and artificial neural networks 

were used for this purpose. The applied approach does not depend on chemical 

standards, is relatively cheap but requires a fair-size calculation effort. 



Keywords: olive oil, sensorial evaluation, classification


Posters 

2-P061 The Proteomic Analysis of Barley Albumins And 

Globulins 

Markéta Laštovičková and Janette Bobáľová 

Institute of Analytical Chemistry,v.v.i, Academy of Sciences of the Czech Republic, 

602 00 Brno, Veveří 97, Czech Republic; 

lastovickova@iach.cz 

A systematic analysis of selected barley (Hordeum vulgare) proteins and glycoproteins 

by is reported here. 

For this purpose, the flour from the malted barley variety Jersey was chosen. 

The different extracts (aqueous, ethanol, salt, TRIS buffers, etc.) of barley proteins 

were compared. Optimized 1-D GE analyses (on 12.5 % SDS gels) revealed the differences 

between the protein patterns of individual barley extracts. 

The mixture of albumins (water-soluble proteins) and globulins (proteins soluble 

in diluted saline solutions) were selected for the detailed investigation. The extract 

was subjected to the separation procedure including Concanavalin A (Con A) 

affinity chromatography, 1-D or 2-D gel electrophoresis and set of desalting and purification 

steps. Both proteins included in Con A non-bound fraction and enriched 

N-glycoproteins in Con A bound fraction were characterized by the combination of 

MALDI-TOF mass spectrometry and bioinformatics. Many important barley proteins 

were identified. 

Acknowledgement: This work was supported from the Ministry of Education, 

Youth and Sports, Czech Republic (Research Centre for the Study of Extract Compounds 

of Barley and Hop No. 1M0570), and Research Plan of Institute of Analytical 

Chemistry, Academy of Sciences of the Czech Republic (AV0Z40310501). 

Keywords: barley, mass spectrometry, proteomics 

2. 1


Posters 

2-P062 Comparative Study of The Protein Composition 

Induced By Malting of Two Barley Varieties 

2. 2 

Markéta Laštovičková, Martina Šopíková, and Janette Bobáľová 

Institute of Analytical Chemistry v.v.i., Academy of Sciences of the Czech Republic, 


lastovickova@iach.cz 

Gel electrophoresis (GE) in combination with mass spectrometry (MS) was used 

for the description of changes in protein profile during malting. Since the composition 

and amount of water-soluble proteins have crucial influence on the suitability 

of grains for its final uses, they were selected as an indicator of changes. The barley 

(Hordeum Vulgare) samples were taken in the course of malting from two varieties: 

Jersey and KM. The experimental conditions for 1-D GE were optimized. The special 

attention was focused on the choice of gel type and kind of staining (Coomassie 

brilliant blue R, Coomassie brilliant blue G, silver). 1-D SDS gels revealed the qualitative 

changes of protein profile. Some of the protein bands disappeared and some 

others arose or were modified during malting. MALDI-TOF MS was used for the 

identifications of these proteins. 

Acknowledgement: This work was supported by the Research Centre for Study 

of Extract Compounds of Barley and Hop No. 1M0570 from Ministry of Education, 

Youth and Sports of the Czech Republic and Research Plan of Institute of Analytical 

Chemistry, Academy of Sciences of the Czech Republic (AV0Z40310501). 

Keywords: 1-D gel electrophoresis, proteins, malting, barley


Posters 

2-P063 fatty acids like markers of processed cheese 

changes during storage 

Blanka Loupancová, Eva Vítová, Hana Štoudková, František Buňka 




blankaloupancova@seznam.cz 

Although the dairy products fall into the class of so called non-acid foodstuffs 

and suitable methods must be used to prolong their durability, the processed cheese 

was proposed as a part of Combat Ratio. The Combat Ratio serves for nutrition 

of soldiers in specific combat situations such as separation from a unit, battle, etc. 

There are special requirements for a minimum durability of individual components 

of Combat Ration which has been set as 24 months depending on an ambient temperature. 

Practically the only method of achieving the 24 month durability of processed 

cheese is the thermo-sterilization. In the article the effect of a defined sterilization 

heating and storage conditions, such as temperature and time, on changes of fatty 

acids contents in sterilized processed cheese was investigated. 

Processed cheeses were stored at 6 ± 2 °C, 23 ± 2 °C and 40 ± 2 °C. The most 

abundant fatty acids such as lauric, myristic, palmitic, stearic and oleic acid were 

elected like markers of changes during storage. The differences between amounts 

of this fatty acids depended of the storage temperature and time of storage were 

observed in this experiment. 

We can conclude that the storage conditions significantly influence the content 

of individual fatty acids in cheeses. The contents of fatty acids in cheeses decreased 

as the consequence of longer time and higher temperature of storage. This fact can 

be probably caused by oxidation reactions of cheese fat. 

Keywords: Processed cheese, fatty acids (optional) 

2.


Posters 

2-P064 Determination of Selected Antioxidant Enzymes 

in Barley and Malt 

2. 

S. Macuchová, I. Márová, R. Mikulíková 

Research Institute of Brewing and Malting, Plc. 

Mostecká 7, 614 00 Brno 

macuchova.s@seznam.cz 

Presence of Reactive Oxygen Species (ROS) negatively affects the sensory stability 

of beer, thus, final quality of beer is related to its antioxidant activity. Antioxidant 

content in beer is substantially dependent on anti-oxidative activity of the primary 

raw materials – barley and malt. In barley antioxidants prevent the undesirable and 

generally negative changes of grain quality during the cropping and stocking. Generally, 

antioxidants can act against ROS by radical trapping, by inhibition of their 

formation or by decreasing of their effects. Complex antioxidant system acts as a 

well-coordinated complex of low molecular weight- and enzyme antioxidants. In 

barley antioxidant enzymes are represented mainly by superoxide dismutase, catalase 

and peroxidase. 

In this work, influence of selected biological and chemical factors on enzyme 

antioxidant activity and total anti-oxidative capacity in barley and malt were studied. 

As a part of this work extraction, optimalization and simplification of methods 

for determination of antioxidant enzyme activity were tested. Finally, changes of 

enzyme activities in barley and malt according to the barley sort and cultivation 

conditions were evaluated. 

Keywords: antioxidants, barley


Posters 

2-P065 Fat content and its quality in rapeseed 

(Brassica napus l) during storage 

Ján Mareček, Martina Fikselová, Helena Frančáková, Zdenka Muchová 

Department of Plant Processing and Storage, Faculty of Biotechnology and Food Sciences, Slovak 

University of Agriculture, Tr. A.Hlinku 2, Nitra, 949 76 Slovakia, e-mail : Jan.Marecek@ 

uniag.sk 

The aim of this work was to evaluate the fat content and its quality of rapeseed 

during the storage. Rapeseed was stored in steel silo during 10 months. Results are 

presented from 3 years of observations (2004–2006). 

Determined parameters of the quality were the contents of dry matter, fat, peroxide 

value, iodine value, acid value, thiobarbituric value. Fatty acids representation 

was determined as follows: myristic, palmitic, palmitoleic, stearic, oleic, linoleic, 

linolenic, arachidonic, behenic and erucic. 

Mean dry matter content during period ranged from 94.34- 94.81 %. Fat content 

during observed period ranged from 43.11-45.52 %. The highest fat content was determined 

at the year 2004. At each year demand on fat content required by Slovak 

Technical Standard was achieved. Peroxide value determined in rapeseed oil was 

optimal (1.97- 3.58 mmol O 2 .kg -1 ). Acid value was higher at year 2006 (2.22 mg KOH. 

g -1 ), higher content of free fatty acids can cause problems with refining. Thiobarbituric 

value was optimal. We can conclude that during observed years were not found 

any important oxidative changes in fat of rapeseed, seems due to optimal conditions 

during the storage. The highest representation within the fatty acids was found at 

oleic acid (61.58 %), linolic acid (24.12 %) and linolenic (6.69 %). Mean content of 

erucic acid was low (0.399 %). 

Keywords: storage, rapeseed, fat quality, fatty acids, oxidation 

2.


Posters 

2-P066 Analysis of Active Substances in Honey – a 

Contribution to Honey Authenticity 

2. 

Ivana Márová, Zuzana Jelénková, Kateřina Duroňová, Petra Ždánská, 

Veronika Ehrenbergerová 

Brno University of Technology, Faculty of Chemistry , 

Department of Food Chemistry and Biotechnology, 


marova@fch.vutbr.cz 

Honey belongs to the oldest delicacies of natural origin. Moreover, honey contains 

many biologically active substances with positive effect on human health. This 

work was focused on study of antioxidant content, enzyme activities, protein and 

saccharide composition in 16 sorts of honey, which differed in geographical and 

botanical origins. To qualitative as well as quantitative analysis of individual lowmolecular 

weight antioxidants RP-HPLC/UV-VIS and LC/MS were used. Antioxidant 

activity was measured by Randox kit, antimutagenic proteprties by S.cerevisiae 

D7 test system. Proteins were analyzed by 1D microfluidic system Experion, saccharides 

by HPLC/RI. Nine flavonoid aglycones were analyzed by HPLC, differend 

conditions were used in order to optimize the detection of those flavonoids which 

could be considered as markers of the floral origin of honey. Significant differences 

between individual sorts of honey were found especially in 5-hydroxymethyl furfural 

content. According to our findings, a set of parameters for honey authenticity 

and quality was proposed. 

Keywords: honey, antioxidants, 5-hydroxymethyl furfural


Posters 

2-P067 Antioxidant and Antimutagenic Activity 

of Dried Fruit, Fruit Teas and Cereal Fruit 

Products 

Ivana Márová, Michaela Barošová, Martina Tomková, 

Hedvika Vondráčková, Kateřina Duroňová, Radka Kočí 





This work was focused on study of antioxidant content and antimjutagenic 

properties of 16 sorts of dried fruit, 5 types of fruit teas and 5 types of herbal teas 

and also in 10 kinds of fruit müsli products. Modern analytical and separation techniques 

were used for analysis of active substances. Total phenolics and total flavonoids 

were analyzed spectrophotometrically. To qualitative as well as quantitative 

analysis of individual flavonoids high-performance liquid chromatography on reverse 

phase with spectrophotometric detection (RP-HPLC/UV-VIS) and LC/MS was 

used. Antioxidant activity was measured by Total antioxidant status kit (Randox). 

Antimutagenic effect was analyzed using Saccharomyces cerevisiae D test strain. In 

cereals also saccharide content by HPLC/RI was analyzed. 

The results show that antioxidant content in dried fruit is relatively high; the 

main contributing derivatives were flavonoids and carotenoids. Total phenolics content 

is higher in herbal teas when compared with fruit teas. From individual flavonoids 

catechin was the most abundant derivative found in most of analyzed herbal 

teas, the lowest amount exhibited quercetin and morin. Cereal products containing 

dried fruits differed in antioxidant levels according to fruit type. As the part of this 

work basic sensory analysis of fruits and cereals was realized. 

Keywords: dried fruits, musli, fruit tea, herbal tea 

2.


Posters 

2-P068 Charactzerization of „Czech Beer“ – a Pilot 

Study 

2. 

Ivana Márová, Renata Mikulíková, Zbyněk Zdráhal, Hana Konečná, 

Kateřina Pařilová, Andrea Halienová 





The aim of presented work is development of modern techniques to qualitative 

and quantitative analysis of some characteristic components of Czech beer. One of 

the main goals is separation and analysis of specific proteins and peptide fragments 

and changes of their content during beer processing. Based on analysis of barley 

variety purity using standard 1D PAGE-SDS detailed characteristics of proteins by 

microfluidic techniques, 2D-electrophoresis, LC/ESI-MS, LC/MS/MS was tested. 

Further aim was qualitative and quantitative analysis of individual phenolics in 

Czech beer by chromatographic techniques (RP-HPLC, LC/MS). In next part volatile 

and bitter substances will be analyzed by gas chromatography connected with 

mass spectrometry techniques in beer and brewing intermediates. All analyses will 

be performed in several kinds of Czech beer in comparison with beers processed by 

different technology. All results will be compared to obtain a set of parameters suitable 

to final characterization of Czech beer. This set could be used in food control to 

authenticity assesment of Czech beer. Finally, proposed general strategy of parameter 

complex analysis could be applicated to beverages authenticity assesment. 

Keywords: Czech beer, proteomics, phenolics


Posters 

2-P069 Tocopherol and Fatty Acid Contents of Selected 

Romanian Cereal Grains 

Cristian Tudor Matea, Ioan Has, Simona Ifrim, Constantin Bele 

University of Agricultural Science and Veterinary Medicine , 

R- 400372, Cluj-Napoca , Romania 

a 2 01@yahoo.com 

HPLC and GC methods were used for the analysis of tocopherols ( α-, γ- and 

δ- ) and fatty acids in 18 selected Romanian cereal grain varietes ( wheat, maize and 

oat ). For the determination of tocopherols , the samples were saponified with KOH 

at 100 0 C followed by extraction with hexane : ethyl ether (7:3 ).An Alltima C18 ( 

5 μm ) and acetonitrile : methanol 50:50 mobile phase were used with fluorescence 

detection. Fatty acids were analyzed using a GC- FID and a capilary column Alltech 

AT-WAX. The richest sources of tocopherols were wheat varietes ( 0,72—1,45 mg 

% ). Total unsaturated fatty acids ( UFA ) ranged from 72-86 % with linolenic, oleic 

and palmitic acid predominant for the maize varities.Total UFA ranged from 57-68 

% for wheat varities and the same predominant fatty acids . 

Keywords: Tocopherols; Fatty acids ; Cereal whole grain 

2.


Posters 

2-P070 Monitoring of Barley Starch Amylolysis by 

Gravitational Field Flow Fractionation and 

MALDI-TOF/TOF MS 

2. 0 

Karel Mazanec and Janette Bobáľová 

Institute of Analytical Chemistry, AS CR, v.v.i. 

Veveří 97, Brno 602 00, Czech Republic 

mazanec@iach.cz 

In barley, starch occurs in form of granules with bimodal size distribution. Enzymatic 

starch granule hydrolysis is one of the most important reactions occurring 

during malting and mashing. In this study, the capacity of gravitational field flow 

fractionation (GFFF) to monitor the amylolysis of starch granules was investigated. 

Isolated starch granules from barley cultivars were treated with amylases. The 

changes in the starch granule size and the bimodal distribution were studied by 

GFFF. Simultaneously, the free sugars released during enzymatic digestion were 

observed by MALDI-TOF/TOF MS. The fractogram changes along with the changes 

in mass spectra reflect a correlation with the progress of enzymatic hydrolysis. The 

results show the interest in utilization of GFFF as a simple and cheap method for 

monitoring of changes of starch granule size distribution during amylolysis. 

Acknowledgement: This work was supported from the Ministry of Education, Youth 

and Sports, Czech Republic (Research Centre for the Study of Extract Compounds 

of Barley and Hop – No. 1M0570) and the Institute Research Plan AV0Z40310501.

2-P071 Wood Biomass in Cosmetics Industry 


Posters 

Radoslav Mikulášik, Jana Kosinková, Aleš Ház, Jarmila Hojerová, 

Svetozár Katuščák, Igor Šurina 




Igor.Surina@stuba.sk 

In this lecture are described possibilities of using of lignocellulosic materials 

(LCM) in cosmetic, pharmaceutical and related industries. Many of substrates or 

products used in these industries are prepared or obtained from plants by different 

methods. One of them is thermolytical decomposition of phytomass as a raw material. 

The thermolysis of wood or of other LCM is mostly used to gain the energy 

covered in. But in this process are also formed different substances using of them 

are not very proficient. 

Different products formed during thermal decomposition of LCM, which can 

be used in cosmetics are characterized and identified by GC-MS. 

Keywords: biomass, phytomass, thermolysis, cosmetics, GC-MS, extractives 

2. 1


Posters 

2-P072 Termogravimetric Analysis of Liquid Pyrolysis 

Products of Wood Biomass 

2. 2 

Radoslav Mikulášik, Aleš Ház, Peter Šimon, Svetozár Katuščák, 

Igor Šurina 




Igor.Surina@stuba.sk 

This work concerns mainly the thermal pyrolytic decomposition of wood biomass 

- lignocellulosic materials (LCM). The products of these reactions are gaseous, 

liquid (pyrolysis condensate or pyro-oil, wood tar) and solid (char). Advantage of 

liquid fraction of wood thermolysis products as a bio-fuel is determined thermoanalytical 

methods. We focused on thermal stability and thermal behavior of these 

products and we suggest some possibilities of their utilization. 

Keywords: biofuels, pyrolysis, thermal stability, TG, DTA, GC-MS


Posters 

2-P073 Analytical Meassurements and Phisicochemical 

Parametrs Determination in Strawberries and 

Raspberries Enhance Production 

Fernando R. Moreno, A. Gago, T. García-Barrera, J. L. Gómez-Ariza 


Campus de El Carmen.21007 Huelva. (SPAIN) 


The safeguard of quality characteristics from harvest up to consumption is an 

essential requirement for the fruit sector. Quality control and authentication of fruits 

and derivatives is a key aspect for consumers and producers. Food authentication 

is the process by which a food is verified as complying with its label description. 

Labelling and compositional regulations, which may differ from country to country, 

have a fundamental place in determining which scientific tests are appropriate for a 

particular issue. Thus the availability of sound analytical methods which can ensure 

the authenticity of foods plays a fundamental role in the operation of modern society. 

In this sense, several analytical parameters have to be taken into account. 

For this reason we develop a project to study about 2000 samples in relation 

with the determination of some analytes in the soil, fruits and plants. The analytical 

measurements involve the determination of elements such as Ca, Mg, K, Na, B, Cu, 

Fe, Ni and Zn; anions: phosphates, nitrates, sulphates, carbonates; solids in suspension, 

pH, redox potential. 

The samples have been studied during six moths in which several agrochemical 

experiments have been developed such as the use of new plastics for the harvest 

and the pollination with bumblebees. 

2.


Posters 

2-P074 Multivariate Methods in Food Analysis 

2. 

Viera Mrázová, Ján Mocák, Katja Šnuderl, Ernst Lankmayr 


University of Ss. Cyril & Methodius, SK-91701 Trnava, Slovakia. 


Methods of multivariate data analysis use various techniques of multidimensional 

statistics for investigation of relations and interactions inside a large table of 

data. They are effectively employed in analysis of food, natural substances, pharmaceutical 

and clinical samples, or environment. Multivariate methods are aimed at 

grouping and classification of objects, which are represented by samples, materials, 

compounds, components of environment, etc. 

The ways of application of multivariate data analysis to classification of various 

food products are here demonstrated. Wine quality, as found by sensorial testing 

(e.g. taste, bouquet and colour), wine variety, and year of production, may be used 

as the main classification criteria. Subjective wine evaluation made by the experts 

can be combined with commonly used chemical and physical-chemical properties, 

measured in analytical laboratory. 

Another example solved in this work is elaboration of classification models 

for pumpkin seed oil samples representing the most widespread brands in Austria. 

Instrumental characterization of the oil samples was performed by the UV-VIS, near 

infrared, infrared and fluorescence spectra. Chemometrical processing of the measured 

data was oriented to selecting the most important spectral features, crucial for 

categorizing the oils into two or more classes according to the pumpkin oil sensorial 

quality evaluated by the experts. The elaborated classification models are then utilized 

for predicting the category, into which unclassified oil samples belong. 



Keywords: multivariate methods, wine, pumpkin seed oil


Posters 

2-P075 Phytochemical Study of Flavonoides from 

Melampyrum Cristatum L. Species 

M. F. Munteanu, L. Vlase, C. Toma 

Universitate de vest”Vasile Goldiş 

The phytochemical studies achieved over the Melampyrum cristatum L. species 

(Scrophulariaceae) on flowers and leaves are qualitative and quantitative, but 

the methods are HPLC/MS, TLC, as well as dosinf after FR X. After the qualitative 

analysis the following results are obtained: by HPLC/MS of methanolic unhydrolysed 

extract from leaves there are isoquercitrine 60.838%, rutozid 3.715%, luteolină 

153.390%, apigenină 15.195%; in the hydrolysed one there quercetol 3.380%, luteolină 

131.092%, kaempferol 1.439%, apigenină 11.235%; in methanolic unhydrolysed 

extract from flowers are isoquercetin 40.930%, rutozid 2.876%, luteolină 140.680%, 

apigenină 18.190%; but in the hydrolysed one quercetol 2.659%, luteolină 11.122%, 

kaemferol 1.642%, apigenină 13.479%; by TLC in the flowers there are apigenină 

0.82%, luteolină 0.74%; but in leaves there are apigenină 0.71% şi luteolină 0.78%. 

The quantitative analyses are made through the method from Cynarae monography 

the quantity of flavonoides found in rutosid in flowers is of 1.755%, but in leaves is 

1.855%, while through the method from Crategus monography the quantity of flavonoides 

found in hiperozida in flowers is 0.722% and in leaves is 0.433%. 

2.


Posters 

2-P076 The Influence of Choice Factors 

on Fermentation of Red Wine 

2. 

J. Omelková, L. Zechmiestrová 


Purkyňova 118, CZ- 612 00 Brno, Czech Republic, 

omelkova@fch.vutbr.cz 

Wine is a product of complex interactions between fungi, yeasts and bacteria 

that commence in the vineyard and continue throughout the fermentation process 

until packaging. The study was oriented on monitoring two technology processes 

for production of red wine from the territory of Czech republic (Velké Pavlovice region). 

The composition of microflora during the stuck fermentation was determined 

in the case, when the wine was exposed to the air and then along the alcoholic fermentation. 

Keywords: Bacteria, fermentation, red wine, yeasts.


Posters 

2-P077 Optimalization of Cultivation Media and 

Hydroxylase Enzyme Production 

by Aureobasidium Pullulans 

J. Omelková, S. Matalová, I. Šimkovic 


Purkyňova 118, CZ- 612 00 Brno, Czech Republic, 

omelkova@fch.vutbr.cz 

The present paper aims to study the influence of medium composition, temperature, 

method of inoculation and process of cultivation on growth of Aureobasidium 

pullulans. In the current work being conducted , cross-linked wheat straw were 

used as substrate. Aureobasidium pullulans was tested for the ability to produce hydroxylases, 

especially laccase and also for the ability to produce release – reducing 

sugars and proteins in basal media. 

Laccase oxidized a redox indicator ABTS ( 2,2 ‘ –azino-di (3-ethylbenzothiazoline-6-sulfonic 

acid ) to form a blue – green cation radical.The method is suitable for 

detecting laccase excreted by mycelia growing on agar and wood-based substrates. 

The method can be adapted for quantitative estimation by spectrophotometry. 

Aureobasidium pullulans were grown in different type of solid media, but less 

growth was observed in the course of liquid cultivations regardless of temperature. 

During the optimalization of the culture medium hydroxylase activity reached higher 

level in the presence of yeast Aureobasidium pulllulans in the glucose medium 

without wheat straw during submerged cultivation. 

Keywords: Aureobasidium pullulans, wheat straw 

2.


Posters 

2-P078 Influence of Modified Biocomposites on 

Production of Extracellular Polysaccharides by 

Immobilized Aureobasidium pullulans 

2. 

V. Ondruška, I. Márová, J. David, L. Vojtová 



Yeast Aureobasidium pullulans produces extracellularly many enzymes and also 

isndutrially significant polysaccharide pullulan. In this pilot study, modified polyurethane 

foams were used as immobilization agents for A. pullulans as well as material 

which could be degraded by yeast cells. First, modified polyurethane foams 

(PUR) were tested as a potential carbon and/or nitrogen source for A. pullulans. PUR 

foams were modified by 10% of carboxymethyl cellulose, 2-hydroxyethyl cellulose, 

acetylated starch and acetyl celulose, respectively. Aureobasidium pullulans cells were 

cultivated in Erlenmeyer flasks in optimal conditions. Samples for pullulan determination 

were taken in 24-hour intervals, quantitative changes of polysaccharide 

content were determined gravimetrically and spectrophotometrically. 

According to our preliminary results it seems that presence of modified polyurethane 

foams in cultivation medium exhibited slight negative effect on pullullan 

as well as biomass production. Thus, immobilization on modified PUR surface is 

probably not suitable for enhanced pullulan production by A.pullulans cells. 

Key words: polyurethane foams, immobilization, pullulan, 

Aureobasidium pullulans


Posters 

2-P079 Fatty Acids Distribution in the Lipid Fractions of 

Calendula officinalis l. Seeds Oil 

Adela Pintea, Francisc Dulf, Constantin Bele, Sanda Andrei 

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca 

Manastur 3-5, 400372, Cluj-Napoca, Romania 

apintea@usamvcluj.ro 

The Calendula seeds oil is currently considered a potential oil seed crop with industrial 

or other functionalities. It is characterized by a high content of the unusual 

conjugated octadecatrienoic acid – calendic acid (18:3c), which give special chemical 

and physical properties. 

The fatty acids composition of main lipid fractions and the sterol content was 

determined by TLC and GC and confirmed by GC/MS. Seeds were analyzed in 

different stages during their maturation. In the seeds collected immediately after 

flower drops the calendic acid represents – 8.62 %, it increased at 26.6 % one week 

after and reached the maximum content of 53 % in the mature seeds. The increasing 

of 18:3c content occurs in the same time with the fast decreasing of linoleic acid and 

a slow decrease of oleic acid, while stearic and linolenic acid remain almost to the 

same values during seeds maturation. Calendic acids is practically concentrated in 

triacylglycerols, and is present in little amount in diacylglycerols and monoacylglycerols. 

Only small amounts of 18:3c was found in the polar lipids and it was absent 

in the free fatty acid fraction. 

Total sterol content was estimated at 95 mg/100 g seeds with the major representatives: 

β-sitosterol (41 %), stigmasterol (18 %), ∆7 – sitosterol (15 %) and campesterol 

10 %). 

Keywords: fatty acids, sterols 

2.


Posters 

2-P080 Lipid Peroxidation Products in Plasma of 

Patients with Chronic Pancreatitis 

2.100 

M. Podborská, A. Lojek, L. Kubala, R. Buňková, I. Márová, 

A. Ševčíková, J. Trna, P. Dítě 

Institute of Biophysics ASCR, v.v.i. 

Kralovopolska 135, CZ-612 65 Brno 

podborska@ibp.cz 

The chronic inflammation of human pancreas (chronic pancreatitis) significantly 

contributes to carcinogenic transformation of pancreatic cells. Inflammation 

is connected with an increased generation of oxygen radicals damaging pancreatic 

tissue. Unsaturated fatty acids are known to be primary targets of radicals. The lipid 

peroxidation leads to formation of mutagenic compounds such as malondialdehyde 

(MDA) and 4-hydroxynonenal (4-HNE). Therefore, the main aim of the study was to 

determine MDA and 4-HNE plasma levels in patients with chronic pancreatitis. 

Lipid peroxidation products MDA and 4-HNE were determined in plasma of 

105 patients with chronic pancreatitis. MDA and 4-HNE adducts with 2,4-dinitrophenylhydrazine 

were extracted with Solid Phase Extraction (SPE) tubes and analyzed 

using HPLC with diode array detector. DSC-C18 tubes revealed the best extraction 

and recovery potential compare to other tested SPE tubes (e.g. DSC-CN). 

There were detected significant levels of plasma MDA (mean 0.42 ± standard 

deviation 0.15 mmol/l) and 4-HNE (0.20 ± 0.07 mmol/l). Plasma levels of MDA did 

not significantly correlate with levels of 4-HNE. 

Herein, we proved formation of lipid peroxidation products in patients with 

chronic pancreatitis. On the basis of this data these patients will be examined periodically 

in the consequent two years. 

Keywords: chronic pancreatitis, lipid peroxidation


Posters 

2-P081 The Effect of Lipophilicity on Antifungal 

Activity of Some 2-Amino and 2- 

Methylbenzimidazole derivatives 


University of Novi Sad, Faculty of Technology 

Bulevar cara Lazara 1, 21000 Novi Sad, Serbia 

sanya@uns.ns.ac.yu 

A series of 2-amino and 2-methylbenzimidazole derivatives were tested in vitro 

for their antifungal activity against yeast Saccharomyces cerevisiae and minimum 

inhibitory concentration was determined for all the compounds. 

Quantitative structure-activity relationship (QSAR) has been used to study the 

relationships between the antifungal activity and lipophilicity parameter, logP, calculated 

by using CS Chem-Office Software version 7.0. The stepwise regression method 

was used to derived the most significant models as a calibration models for predicting 

the inhibitory activity of these class of molecules. 

The models were validated by leave one out technique as well as by the calculation 

of statistical parameters for the established theoretical models. The best QSAR 

model for prediction of antifungal activity of the investigated series of benzimidazoles 

was developed. High agreement between experimental and predicted inhibitory 

values was obtained. 

The results of this study indicate that the lipophilicity parameter has a significant 

effect on antifungal activity of this class of compounds, which simplify design 

of new biologically active molecules. 

Keywords: benzimidazole, lipophilicity 

2.101


Posters 

2-P082 QSAR Models for Predicting the Antibacterial 

Activity of Some 1-Benzylbenzimidazole 

Derivatives 

2.102 

Sanja O. Podunavac-Kuzmanović, Dragoljub D. Cvetković, D.Barna 




In the present study a series of 1-benzylbenzimidazole derivatives were 

tested in vitro for their antibacterial activity against Pseudomonas aeruginosa. The 

tested compounds displayed in vitro antibacterial activity and minimum inhibitory 

concentration (MIC) was determined for all the compounds. 

Quantitative structure activity relationship (QSAR) analysis was applied to 

find out the correlation between the various physicochemical, steric, electronic, and 

structural molecular descriptors of the compounds and their inhibitory activity. The 

molecular descriptors were calculated by use of different software products and 

stepwise regression method was used to derive the most significant models. 

The results showed that the antibacterial activity could be modeled using the 

different values of molecular descriptors. The low residual activity and high crossvalidated 

r2 values (r2 ) observed indicated the predictive ability of the developed 

cv 

QSAR models. It was revealed that lipophilic parameter was found to have significant 

influence on the antibacterial activity and this study provides an insight to design 

new molecules. 

Keywords: benzimidazole, antibacterial


Posters 

2-P083 Antimicrobial Investigations of Nickel(II) 

Complexes With Some 1-Benzylbenzimidazole 

Derivatives 





In the paper the antimicrobial activity of nickel(II) complexes with two 

series of benzimidazoles was investigated. The first one was based on 1-benzyl- 

2-aminobenzimidazole, and the second one contained 1-benzyl-2-amino-5,6dimethylbenzimidazole. 

Antimicrobial activities of the complexes were evaluated in vitro against three 

gram-positive bacterial strains (Bacillus cereus, Staphylococcus aureus and Sarcina 

lutea) and one gram-negative isolate (Pseudomonas aeruginosa). All the investigated 

compounds displayed antimicrobial activity against very persistent microorganisms, 

whilst the inhibitory activity is upper against gram-positive than gram-negative 

bacteria. Minimum inhibitory concentration (MIC) was determined for all the 

complexes. The effect of ligand and complex structure on the antimicrobial activity 

is discussed. 

Keywords: benzimidazole, antimicrobial 

2.10


Posters 

2-P084 Cheese Species Identification by Multivariate 

Analysis of Elemental Data 

2.10 

Martin Polovka, Milan Suhaj, Mária Koreňovská 

Department of Chemistry and Food Analysis, VÚP Food Research Institute 

Priemyselná 4, P. O. Box 25, SK-824 75 Bratislava, Slovak Republic 

polovka@vup.sk 

Multivariate statistical techniques, e.g., cluster, principal component, factor, 

and canonical discriminant analysis, were used for the mutual differentiation and 

classification of 54 cow’s, 116 sheep’s and 20 goat’s cheeses of Slovak origin, using 

Ba, Cr, Cu, Hg, Mg, Mn, Ni and V as the elemental markers, determined by atomic 

absorption spectroscopy (AAS). 

Using the cluster analysis, preliminary results were obtained, indicating the 

partial species differentiation of cheeses. Principal component and factor analysis 

seemed to be effective methods not only for data visualisation but also for the efficient 

differentiation of examined cheese species. Canonical discriminant analysis revealed 

more than 93% successful differentiation and correct classification of cheese 

samples. Stepwise discriminant analysis selected Mg, Cu, Ni and Mn as the best set 

of markers for Slovak cheese species discrimination and identification of their potential 

origin. As the main limiting factor, the composition of milk used for cheeses’ 

production must been also taken into account. 

Keywords: cheese, origin, elemental markers


Posters 

2-P085 Optimalization of Method for Quantification of 

Streptococcus Mutans to Dental Materials 

Ilona Prudíková, S. Matalová, Jiřina Omelková, Josef Jančář 

Institute of Materials Chemistry (IMC), Brno University of Technology, Faculty of Chemistry, 


xcprudikova@fch.vutbr.cz 

Microbial adhesion to the surface of composite resins and other restorative materials 

is an important parameter in the aetiology of dental caries. 

The aim of this study was to optimized the method for quantification of 

plaque deposition. The test surfaces used were a commercial microhybrid composite 

(Adoro, Ivoclar Vivadent), a commercial D3MA resin-based material (Advanced 

Dental Materials) and a commercial ceramic material (Ivoclar Vivadent) as reference 

material. 

The adhesion was tested with Streptococcus mutans. Microbial culture was inoculated 

into sterile test tubes in BHIB with tested specimens and incubated anaerobically 

for 17 hours. 

For the determination of bacterial adhesion the modified method of Biuret was 

used. Protein concentration was obtained spectrophotometrically at 540 nm. 

Keywords: adhesion, dental materials 

2.10


Posters 

2-P086 BFRs Profiles in Breast Milk and Adipose Tissue 

Samples: Case Study Concerned with Czech 

Population 

2.10 

Jana Pulkrabová, Petra Hrádková, 

Michaela Nápravníková, Jana Hajšlová, Jan Poustka 

ICT, Prague, Department of Food Chemistry and Analysis 

Technická 3, 16628 Prague 6, Czech Republic 

jana.pulkrabova@vscht.cz 

In the recent two decades, growing attention has been focused on BFRs, a group 

of “emerging” halogenated POPs, which, similarly to chlorinated POPs, tend to accumulate 

in lipid rich tissue across the food web. The main aim of the presented 

study was to investigate levels and profiles of (i) BFRs represented by PBDEs and 

HBCD and (ii) “classical” organochlorine contaminants occurring in the adipose 

tissue of people living in the Czech Republic and in human breast milk from Czech 

mothers. To our knowledge, this is the very first study reporting PBDEs levels in 

adipose tissue samples of Czech citizens together with similar studies conducted 

elsewhere in the world. The results clearly showed ubiquitous occurrence of BFRs 

in the environment of general Czech population. While BDE 153 was the most abundant 

congener in examined adipose tissue samples, followed by BDE 47. Opposite 

ratio of these major PBDEs was found in human breast milk. The diversity in kinetics 

of individual congeners in human body was documented this way. In spite of 

an extensive use, BDE 209 was detected in only few adipose tissue samples and /or 

human breast milk. 

This study was supported by project BIOBROM (2B06151) and MSM 6046137305, 

both funded by MSMT of the Czech Republic 

Keywords: POPs, BFRs, adipose tissue, breast milk

2-P087 Iodine in the Czech Market Milk 


Posters 

Jana Řeháková, Radek Kavřík, Irena Řehůřková, Jiří Ruprich 

National Institute of Public Health in Prague, Centre for Hygiene of Food Chains 

Palackého 3a, 612 42 Brno, Czech Republic 

kavrik@chpr.szu.cz 

Iodine is very important element for human body, inside of context with function 

of thyroid gland. In past there was observed iodine deficit in the Czech Republic. 

The National Institute of Public Health in Prague, Centre of Hygiene of Food 

Chains in Brno is involved in „The Project on Dietary Exposure to Selected Chemical 

Substances“. The objective of the project is to describe the dietary exposure of the 

population of the Czech Republic. In the framework of this project iodine has been 

monitored since 1998. 

It was founded that milk is the most important source of iodine. That can be a 

hazard of high intake value of iodine for children less than ten years old, because in 

this age are both milk and dairy produces very important parts of diet. EC Regulation 

No. 1459/2005 decreased amount of iodine in complete feed for dairy cows and 

layers from 10 mg/kg to 5 mg/kg. 

For determination of iodine was used Sandell-Kolthoff spectrofotometric method. 

The principle of this method is in catalytic function of iodide to oxidation-reduction 

reaction Ce 4+ and As 3+ . Limit of quantification is 15 μg/kg. The method is 

accredited by Czech Accreditation Institute. 

According to the monitoring plan, every year, in May and November, 24 samples 

of milk (12 half-fat milks and 12 defatted milks) was purchased from shops in 

Czech Republic. These samples were analysed as one composite sample. Average 

content of iodine in milk is about 300 ug/kg. Since 2007 milk samples are analysed 

individually to get imagination about distribution of iodine concentration. 

Keywords: iodine, milk 

2.10


Posters 

2-P088 Monitoring of Total Mercury Content in Foods 

Consumed by the Czech Population 

During 1994–2005 

2.10 

J. Řeháková, I. Řehůřková, J. Ruprich, J. Kalvodová, D. Matulová 

National Institute of Public Health - Centre for the Hygiene of Food Chains, Palackého 3a, 

Brno 612 42 Czech Republic, www.chpr.szu.cz 

rehakova@chpr.szu.cz 

Mercury belongs to group of heavy metals. For human organism it is entirely 

toxic element. Mercury can be found in the nature in various forms with different 

toxicity. The most important intake of mercury into the organism takes place via the 

digestive tract. Hence, systematic monitoring of occurrence and concentration in 

foodstuffs is important. 

As a significant contaminant mercury (total) is monitored within the framework 

of „The Project of Dietary Exposure of the Czech Population to Selected Chemical 

Substances“ carried out by the Centre for the Hygiene of Food Chains in Brno 

since 1994. The aim of this programme is assessment of the dietary exposure of the 

Czech population to chemical substances, comparison to exposure limits, estimation 

of burden of the population and specification of health risks. 

Mercury content is determined in samples of food, which represent so-called 

food basket defined for average consumption of Czech population. Foodstuffs significant 

by its mercury content are sea fish and freshwater fish, pork liver, offal, 

mushrooms. With regard to consumption in term of contribution to exposure dose 

sea fish and freshwater fish, potatoes and rice are significant. 

The FAO/WHO JECFA evaluated PTWI for total Hg and set 5 ug/kg b.w./week 

in 1978. Observed average exposure dose estimated for Czech population reached 

1,9 % PTWI. 

Mercury content is determined by AAS method using principle of selective system 

AMA 254 with solid sample dosing. 

Keywords: mercury, dietary exposure


Posters 

2-P089 The Influence of Surface Characteristics on 

Bacterial Cell Adhesion 

Olga Schreiberová, Tereza Krulikovská, Jitka Hrdinová, Jan Masák, 

Alena Čejková, Vladimír Jirků 


Department of Fermentation Chemistry and Bioengineering 

Technická 5, 166 28 Praha 6 - Dejvice 

olga.schreiberova@vscht.cz 

Cell adhesion is an important feature of cell behaviour in natural environments. 

It is also important in many branches of human activities. Adhered cells are 

known to possess properties that enable them to withstand stressful environment. 

This characteristic is highly important in pollutant degradation. The properties of 

cell wall and envelopes are the important features that determine the ability of cells 

to adhere to a carrier with specific properties. 

The aim of our work was the determination of external conditions that lead to 

cell adhesion. The cell wall properties and cell adhesion on specific materials were 

evaluated. 

The studied microorganism was gram-positive bacteria Rhodococcus erythropolis 

that is able to utilize phenol and other toxic pollutants as a sole carbon source. Its 

ability to adhere to silicone, modified glass and teflon was determined. 

The methods employed in cell wall characterization were: cell wall hydrophobicity 

measurement, X-ray photoelectron spectroscopy analysis, cell wall protein 

and saccharides determination. 

Keywords: cell wall properties, biodegradation, cell adhesion 

2.10


Posters 

2-P090 HPLC Analysis of Flavonoids 

from Satureja Hortensis L. 

2.110 

Maria-Loredana Soran, Bros Ildiko, Constantin Bele, Emanoil Surducan, 

Vasile Surducan, Cristian Tudoran 

National Institute of Research & Development for Molecular and Isotopic Technology, 

65-103 Donath Street, 400293 Cluj-Napoca, Romania; 

loredana_soran@yahoo.com 

The determination of flavonoids from Satureja hortensis L. was performed by 

High-Performance Liquid Chromatography (HPLC) on a RP-C18 column, using an 

HPLC Shimadzu equipped with photodiode array detector. The separation method 

involved one linear gradient and one isocratic step. Solvent A was methanol and 

solvent B was water with 0.1% acetic acid. At the begin, the elution was performed 

with 5% methanol that increase linear to 42% in 35 minutes and than 42% methanol 

for 15 minutes. The analytical column was maintained at 48 0 C and the elution was 

performed with 1 ml/min flow-rate. The injected sample volume was 20 μl. 

The standard solutions were analyzed and the calibration curves were drawing 

for to determine the flavonoids from our plant. 

Keywords: HPLC, flavonoids


Posters 

2-P091 Selection of Suitable Microorgamisms for 

Preparation of Lactic Acid Fermented Cabbage 

Juice 

Ivana Šimonová, Jolana Karovičová, Mária Greifová, Zlatica Kohajdová 

Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, 

Slovak University of Technology 

Radlinského 9, 812 37 Bratislava, Slovak Republic 

ivana.simonova@stuba.sk 

The conditions for lactic acid fermentation based on a cabbage juice and four 

probiotic lactic acid bacteria has been studied. All cultures showed good biochemical 

activity in the cabbage juices. Addition of prebiotic preparation into the cabbage 

juices has been studied also. 

We tested the growth of probiotics cultures in the three medium (medium 

A-MRS broth, medium B-MRS broth with addition 2 % prebiotic preparation and 

medium C-MRS broth, the glucose was substituted prebiotic preparation) and the 

metabolites from the cultivation of probiotic bacteria were determinated by HPLC 

method with RI detection. 

The results show, that tested probiotic bacteria and addition of prebiotic preparation 

were suitable for preparation lactic acid fermented cabbage juice with functional 

properties. 

Keywords: functional food, lactic acid fermentation 

2.111


Posters 

2-P092 Using of Hydrocolloids for Delaing Staling of 

Bakery Products 

2.112 

Ivana Šimonová, Zlatica Kohajdová, Jolana Karovičová 




zlatica.kohajdova@stuba.sk 

Nowadays, the use of additives has become a common practice in the baking 

industry...The effect of different hydrocolloids on the rheological properties of composite 

flour dough, quality of final products and shelf life was investigated. 

Addition of these compounds concluded in higher water absorption capacity (from 

60.5 to 68.3 %) and dough stability (from 6.5 to 14 min). The incorporation of hydrocolloids 

into the loaves also influenced volume, cambering and sensory acceptance of final 

products in different ways. Baked goods staling evaluated during 72 h storage period 

through bread firmness values showed, that loaves prepared with hydrocolloids extent 

products contained celullose derivate were soften when control sample. In conclusion, 

guar gum could be recommended as improver in the bread-making performance owing 

to its good rheological, sensory and crumb softening effects. 

Keywords: hydrocolloids, staling


Posters 

2-P093 Influence of Ph on the Rheology Properties of 

Tomato Ketchups 

Ivana Šimonová, Jarmila Lehkoživová, Jolana Karovičová, Jarmila Hojerová, 

Zlatica Kohajdová, 




jarmila.lehkozivova@stuba.sk 

The aim of this work was investigated the influence of pH on the rheological 

properties of 3 samples of the formulated tomato ketchups. In recent years rheological 

properties of food products have become increasingly important in the formulation 

of food and the optimization of food processing. 

The tomato ketchup samples were prepared from tomato paste (TSS 28-30%) of 

the company Kolagrex Int., s.r.o. with ingredients (resistamyl, isosweet, guar gum, 

vinegar and salt) and pH was modified at level 3.5, 4.0 or 4.5 (the most common pH 

levels of tomato ketchups). The mixture was then stirred 5 min and heated until the 

temperature 85°C reached. 

The rheological measurements were carried out using the rotational viscometer 

(controlled rate mode) HAAKE Viscotester VT 550 (Haake, Karlsruhe, Germany) 

with coaxial cylinder measuring sensor systems MV DIN. The Viscotester VT 550 

was computer-operated with the application software. 

The results were indicated that these samples of tomato ketchup behave as non- 

Newtonian fluids with pseudoplastic and thixotropic characteristics. The apparent 

viscosity was decreased with increasing pH of tomato ketchups at all temperature 

range 5-90°C. 

Keywords: rheological properties, tomato ketchup 

2.11


Posters 

2-P094 Distribution of Diazinon in Various Tissues and 

its Effect on Serum Cholinesterase after an 

Intraperitoneal Administration 

2.11 

Branislav Šiška, Jozef Golian, Robert Toman, Andrea Krajčírová 

Fakulty of Biotechnology and Food Sciences, Slovak Agricultural University in Nitra 

Trieda A. Hlinku 2, 949 76 Nitra 

branislav.siska@gmail.com 

The aim of this study was to evaluate the distribution of diazionon in various 

tissues and its effect on serum cholinesterase catalytic activity after an intraperitoneal 

administration. 

Laboratory rats were randomly divided into 2 groups. Each group consisted of 

10 males. Animals in the first group were administrated with diazinon 20 mg.kg -1 

bw. intraperitoneally in physiological solution. The second group served as a control 

group and was administrated only with the physiological solution. 24 hours 

after the administration of tested substance, animals were sacrificed, blood samples 

were taken from hearts and samples of livers, kidneys, muscles and fat tissue were 

taken during the autopsy. 

Catalytic activity of cholinesterase was determined with the using of Bio-La- 

Test ® . The amount of diazinon in tissues was determined with using of gas chromatography 

with mass spectrometry. 

We observed that average amount of diazinon in samples of livers was under 

the detection limit, in samples of kidneys and muscles was slightly increased and 

in samples of fat tissue was significantly increased. We also observed significant 

decrease of cholinesterase activity in comparison with control animals. 

Keywords: diazinon, cholinesterase activity


Posters 

2-P095 Identification of Bacterial Strains of Species 

Lactococcus Lactis in Hard Cheeses using PCR 

A. Španová , J. Herzogová, B. Rittich 



Purkyňova 464/118, 612 00 Brno, Czech Republic 

Species-specific PCR primers 1 PALA4 and PALA14 (targeted on acm A gene encoding 

N- acetylmurimidase specific for L. lactis) were used for identification strains 

of species Lactococcus lactis in hard cheeses, The 16S rDNA-targeted PCR primer 

pair 27f-L1a was used 2 for distinction between Lactococcus lactis subsp. lactis and 

Lactococcus lactis subsp. cremoris. Falsely negative results can occur in PCR identification 

due to the presence of extracellular PCR inhibitors in tested real samples. Pre- 

PCR processing procedures have been developed to remove or reduce the effects of 

PCR inhibitors. Bacterial DNA was isolated from hard cheeses samples and crude 

cell lysates from bacteriocin producing strains by magnetic carriers P(HEMA-co- 

EDMA) containing carboxyl groups. DNA was reversibly bind on carrier surface in 

the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium 

chloride 3 . Phenol extraction of DNA was used as control. The PCR products were 

separated and identified using electrophoresis in agarose gel. 

1 G. Buist, J. Kok, K.J. Leenhouts, M. Dabrowska, G. Vemena, A.J. Haandrikman. 

J. Bacteriol. 177 (1995) 1554. 

2 R. K. Barakat, M.W, Griffiths L.J. Hartus. Internat. J. Food Microbiol. 62 (2000) 

83. 

3 J. Křížová, A. Španová, B. Rittich, D. Horák, J. Chromatogr. A 1064 (2005) 247. 

This research has been supported by grant National Agency for Agricultural 

Research 1G57037 a long-term research program of the Ministry of Education, 

Youth and Sports of the Czech Republic (project No. MSM 0021622415). 

2.11


Posters 

2-P096 Identification of Bacterial Strains of Species 

Streptococcus thermophilus using PCR 

2.11 

A. Španová, M. Tycová, B. Rittich 

Brno University of Technology, Faculty of Chemistry, Department of Food Chemistry and 

Biotechnology, Purkyňova 464/118, 612 00 Brno, Czech Republic 

Polymerase chain reaction (PCR) is molecular diagnostic method which allows 

the identification of lactic acid bacteria used in food industry. In the present study 

species-specific PCR primers 1 (targeted on highly conserved 16S rDNA region) were 

used for identification of 23 strains of species Streptococcus thermophilus collected 

in Culture Collection of Dairy Microorganisms Laktoflora (CCDM, Tábor, Czech 

Republic). Bacterial DNA was isolated from crude cell lysates by magnetic microspheres 

P(HEMA-co-EDMA) containing carboxyl groups. DNA was reversibly bind 

on their surface in the presence of high concentrations of poly(ethylene glycol) (PEG 

6000) and sodium chloride 2 . Phenol extraction of DNA was used as control. The 

PCR products (968 bp) were separated using electrophoresis in agarose gel. 

1 S. Lick, K. Dreschner, K.J. Keller. Appl. Environ. Microbiol. 67 (2001) 4137. 

2 J. Křížová, A. Španová, B. Rittich, D. Horák. J. Chromatogr. A 1064 (2005) 247 

This research has been supported by a long-term research program of the 

Ministry of Education, Youth and Sports of the Czech Republic (project No. MSM 

0021622415).


Posters 

2-P097 Study of Oxidation Stability of Selected 

Vegetable Oils 

Hana Štoudková, Monika Maxová, Jiří Kučerík, Jana Zemanová 


Institute of Food Science and Biotechnology 


stoudkova@fch.vutbr.cz 

Vegetable oils are obtained from different parts of oilseed plants by cold 

expression and subsequent extraction and purification. Since long ago, oils belong 

to among basic cosmetic preparations and use in pharmaceuticals, food industry 

and other industrial purposes. They embody the whole series of authenticated positive 

efects on human organism and they are used as a part of food supplements. 

Oxidation processes influence quality of oils and fats. Characteristic changes 

associated with oxidative deterioration include development of unpleasant tastes 

and odours as well as changes in color, viskosity, density or solubility. 

The thermal stability of different vegetable oils refined (Grape seed, Crude 

Linseed, Castor, Almond, Soya bean, Pumpkin seed, Jojoba, Avocado, Apricot kernel, 

Rice bran, Sunflower, Corn and Olive) were studied. As the analytical method 

Differential Scanning Calorimetry (DSC) was employed. The order of steadiness 

of vegetable oils was identified by statistical comparison of record gained from 

various temperature extrapolation and reciprocal comparison of steadiness of given 

oils. The order of the most stable oils (castor and olive) and the least stable 

oil (linseed) depending on extrapolation and reciprocal comparison did not differ. 

The non-isothermal method was employed with the aim to determine the induction 

period of vegetable oils. 

Keywords: vegetable oils, DSC, induction period, oxidation 

2.11


Posters 

2-P098 Antioxidant Properties of Grape Skins Extracts 

Investigated by EPR and UV-VIS Spectroscopy 

2.11 

Lenka Šťavíková , Martin Polovka 



Purkyňova 118,CZ-612 00 Brno, Czech Republic. 

stavikova@fch.vutbr.cz 

Nowadays, the growing attention is focused on the improvement of human 

health by the consumption of food or food supplements rich in antioxidants. Grape 

skins contain a plenty of different flavonoids, e. g., quercetin, catechins, flavonols, 

anthocyanidins, phenolic acid derivatives and other compounds. They are recently 

of great interest due to their potential role in the “French Paradox” phenomenon. 

As the beneficial effects of wine consumption are ascribed just to them, they are also 

used for different therapeutic purposes. Most of these compounds are released to 

wine during its production. 

In this contribution, the complex study of grape skin alcoholic and water 

extracts, prepared from two wine grape varieties, Svatovavřinecké (St. Laurent) and 

Alibernet of Moravian origin is presented. Extracts were prepared from different 

amounts of either crude dried grape skins or lyophilized grape skin powders (0.5, 

1.0 and 1.5 g) using the solvent extraction to methanol, ethanol and water at different 

temperature from 40 up to 120°C. 

Antioxidant activity of extracts was tested by EPR spectroscopy in Fenton sys- 

tem (H2O2/Fe2+) generating reactive radicals ( • OH, O 2 

–• , •CH3) followed by spin 

trapping technique, using 5,5-dimethylpyrroline-N-oxide (DMPO) as spin trap. In 

addition, radical scavenging activity of extracts was assessed applying 2,2-diphenyl-1-picrylhydrazyl 

( • DPPH) free radical and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic 

acid) cation radical (ABTS •+ assays. Total phenolic content (TPC) of 

individual extracts was determined using the Folin-Ciocalteu assay and their tristimulus 

color values (CIE Lab) were estimated, using the UV-VIS spectrophotometer. 

In addition, pH values of all extracts were also measured. All the data obtained 

were subsequently correlated and discriminated, using the multivariate statistics, 

involving the canonical discriminant analysis, principal component analysis, and 

canonical correlation analysis, respectively. ... 

The extend of this abstract exceeded the allowed size and therefore had to be clipped.


Posters 

2-P099 The Impact of Fermentaion Process on Amino 

Acids Profile in White Wine Veltlínské Zelené 

Lenka Šťavíková, Miroslav Hrstka 



Purkyňova 118,CZ-612 00 Brno, Czech Republic. 

stavikova@fch.vutbr.cz 

Amino acids represent 30-40% of total wine nitrogen. It is generally accepted, 

that they may act as nutrients for bacterial growth during the secondary fermentations. 

They can be either partially or totally metabolized by yeast during the growth 

phase, excreted by living yeasts at the end of fermentation or released by proteolysis 

during autolysis of dead yeast. On the contrary, their production by enzymatic 

degradation of the grape proteins is known. Furthermore, it is well-known that the 

amino acid content of wine is dependent on the fertilization, climatic conditions, 

duration of skin maceration in the must, field treatments and on other factors. 

In this work, the impact of spontaneous and controlled fermentation on amino 

acid profile in white wine Veltlínské zelené (Vitis vinifera) was observed. The ion 

exchange chromatography with post-column derivatization with ninhydrin and 

spectrophotometric detection were utilized as detection system. The sampling and 

subsequent analysis was performed every other day continuously, starting from cider 

up to final young wine. 

Using this approach, 23 different amino acids were identified. The arginine, 

alanine, proline, glutamine and γ-aminobutyric acid were the most dominant. Their 

concentration in the samples obtained by controlled fermentation range from 302 

mg.l -1 up to 1578 mg.l -1 , whereas in those obtained by spontaneous fermentation, 

from 289 mg.l -1 up to 1371 mg.l -1 . During the spontaneous fermentation process, the 

slight increase of majority of amino acids’ concentration to the maximum with gradual 

decrease after reaching it was observed. On the contrary, during the controlled 

fermentation, rapid decrease of amino acids content without any meaningful alterations 

for any of them was noticed. The most significant concentration differences 

between controlled and spontaneous fermentations were observed for asparagine, 

proline, glycine, cysteine and citruline. 

Keywords: wine, amino acids, fermentation, ion exchange chromatography 

2.11


Posters 

2-P100 Characterization of Neutral Lipid Composition 

of Nigella Sativa L. Seeds Oil 

2.120 

Claudia-Crina Toma, Adela Pintea, Constantin Bele, Francisc Dulf . 

Universitatea de Vest “Vasile Goldis” Arad 

Feleacului, 2 Arad 340041, Romania 

claudiatoma200 @yahoo.com 

Nigella sativa L. seeds are used as food ingredients but also as a medicinal plant 

with various pharmaceutical effects. The total oil content of Nigella seeds oil represents 

31 % of fresh weight and the main fraction – 97 % - consists in the neutral 

lipids. 

Triacylglycerols, diacylglycerols, monoacylglycerols and esterified sterols were 

separated by column chromatography and thin layer chromatography. The fatty acids 

(as fatty acids methyl esters) profile was determined in each fraction by GC/FID. 

Data were compared with fatty acids of the total oil and those of the polar lipids. 

Triacylglycerols are the main fraction of the neutral lipid class. Their fatty acid 

profile is very similar with the total oil, with: 12 % palmitic acid, 22 % oleic acid, 57 

% linoleic acid and 2.4 % eicosadienoic acid. The latest one is an unusual fatty acids, 

considered a taxonomic marker of Nigella species. Diacylglycerols and monoacylglycerols 

have a similar profile, but with lower amounts of linoleic acid (51 and 50 

%) and higher amount of stearic an oleic acid. The most saturated fraction was the 

esterified sterols with 17, 6 % palmitic acid and, 4, 5 % of stearic acid. Polar lipid 

fraction also contains important amounts of palmitic and stearic acid. 

The highest content of eicosadienoic acid was found in the polar lipid fraction 

(4 %) while the lowest is in the esterified sterols (2 %). 

Nigella sativa represents valuable edible oil, with high content of polyunsaturated 

fatty acids and phytosterols. 

Keywords: neutral lipids, fatty acis


Posters 

2-P101 Identification of Viable Lactobacillus Cell in 

Fermented Milk Products. 

S. Trachtová, A. Španová, B. Rittich 

Brno University of Technology, Faculty of Chemistry, Department of Food Chemistry and 

Biotechnology, Purkyňova 464/118, 612 00 Brno, Czech Republic 

The combination PCR and ethidium monoazide (EMA-PCR) is a new method 

for selective distinction between viable and dead bacterial cells. The general application 

of EMA is based on penetration into ’dead’ cells with compromised membrane 

(cell wall systems) integrity. DNA bound to EMA cannot be PCR amplified. 

Live cells remain intact and only DNA from viable cells can be amplified. 

Experiments were performed using probiotic bacterial strain Lactobacillus paracasei 

subsp. paracasei CCDM 211/06 of human origin. The experimental conditions (time of 

cell incubation with EMA, and cell irradiation) were optimized. In the next step instead 

for live-dead cell distinction propidium monoazide was used. The Lactobacillus species 

specific primers were used for DNA amplification. The PCR products (250 bp) were 

separated using electrophoresis in 1.8 % agarose gel. The method was applied for live 

and dead cells identification in milk product (yoghurt). 

Keywords: ethidium monoazide, viable and dead bacterial cells 

2.121


Posters 

2-P102 Dynamic of Selected Pesticides Applied to 

Apples Protection During Pre-Harvest and 

Post-Harvest Period 

2.122 

Jana Urbanova, Ondrej Lacina, Jakub Schurek, Miroslav Lansky 

and Jana Hajslova 

Department of Food Chemistry and Analysis, Institute of Chemical Technology, 

Prague 6, Czech Republic 

jana.urbanova@vscht.cz 

Pesticides represent an important group of agrochemicals used for food crop 

protection. Even if applied in accordance with Good Agriculture Practice (GAP) 

principles, some residues can be detected in treated crops at the harvest time. With 

regard to health hazard this issue is mainly of concern when crops are used for 

baby food production. Infants and young children are the most vulnerable group 

of human population, therefore EU uniform residue limit 0.010 mg/kg has been 

established for any pesticide residue in processed baby food to reduce the healthrisk. 

To select pesticides leaving minimal residues in apples intended for baby 

food production, field experiments involving four apple varieties (Gloster, Golden 

Delicious, Idared, Melrose) were established. Pesticide preparations containing 

following active ingredients were applied between April and June: (i) fungicides 

(bitertanol, captan, cyprodinil, difenoconazole, dithianon, dodine, fluquinconazole, 

flusilazole, mancozeb, metiram, penconazole, pyrimethanil, tetraconazole, thiram) 

and (ii) insecticides (acetamiprid, etofenprox, fenoxycarb, flufenoxuron, methoxyfenozide, 

thiacloprid, triazamate). For post-harvest storage a range of fungicides 

was used: dithianon, thiram, trifloxystrobin. High performance liquid chromatography 

coupled with tandem mass spectrometry (HPLC-MS/MS) was employed for 

analysis of residues in apples at the harvest time and during storage (three and 

five months after harvest) and for ethylenbisdithiocarbamate (EBDC) fungicides 

were used solid phase microextraction followed by gas chromatography with mass 

spectrometry (SPME-GC-MS). 

This study was carried out within the project NAZV 1G46073. 

Keywords: pesticide, apple


Posters 

2-P103 Relationship between Xanthohumol, Polyphenols 

and flavonoids content in hop Leaves and Cones 

with Regard to Cultivar and Vegetation Period 

Eva Ürgeová, Peter Kulacs, Ľudovít Polívka 

Department of Biotechnologies, Faculty of Natural Sciences, 

University of St. Cyril and Methodius in Trnava, 

Nám. J. Herdu 2, 917 01 Trnava, Slovakia; 

eva.urgeova@ucm.sk 

The aim of this work was testing of content of some secondary metabolites in 

different cultivars of hop during vegetal period. The first samples we tested before 

the flowering and the second at the end of vegetal period. The experiment took place 

in 2007. Some relevant data from 2006 were included for comparison. The content of 

secondary metabolites, phenol substances, flavonoids and especially xanthohumol 

in extracts from hop leaves and cones was definite by standard analytical methods. 

The content of the secondary metabolites in leaves depended on the vegetal 

period at first. The climatic condition for content of secondary metabolites of hop 

cones was met. 

Keywords: polyphenols, flavonoids, xanthohumol, hop 

2.12


Posters 

2-P104 Amino Acid Composition of Non-Transgenic 

and Ovalbumine Expressing Transgenic Alfalfa 

(Medicago sativa). 

2.12 

Eva Ürgeová, Juraj Faragó, Miklós Fári, Natália Faragová 

SARC, Research Institute of Plant Production 

Bratislavská cesta 122, 921 68 Piešťany, Slovak Republic 

farago@vurv.sk 

Alfalfa is the world’s most important forage crop grown as a source of proteins 

in animal feed. However the proteins of alfalfa are limited in the essential amino 

acids (EAA) methionine (Met) and cysteine (Cys). Several different transgenic approaches 

have been used to improve the nutritional quality of this crop, such as 

expression of heterologous high-Met proteins or metabolic engineering of EAA 

biosynthetic pathways. We introduced into alfalfa the gene Ov from Japanese quail 

coding for high Met+Cys containing protein ovalbumine using the Agrobacterium 

tumefaciens-mediated genetic transformation method. More than 100 putatively 

transgenic lines were regenerated in in vitro culture, and the transgenic nature of 

regenerants was proved by gene expression bioassays, PCR analysis and Western 

blot analysis. 

HPLC analysis was applied to study the amino acid composition of leaf+stem 

samples of 32 transgenic plants and 2 non-transgenic isogenic and 6 non/transgenic 

control plants. Met+Cys content in control plants averaged 0.23 % DW and 0.25 % 

DW in non-transgenic isogenic plants. Transgenic plants had variable contents of 

Met and Cys, ranging from 0.04 to 0.36 % DW for Met and 0.06 - 0.18 % DW for 

Cys. We could identify 3 transgenic genotypes that contained 188-233 % Met+Cys 

comparing the control plants. 

Keywords: essential amino acids, HPLC, transgenic plants


Posters 

2-P105 Sea Buckthorn – Source of Vitamin C and 

Efficient Antioxidant 

Milena Vespalcová, Dana Vránová, Martin Polovka, Jiřina Endstrasserová, 

Vojtěch Řezníček 

Faculty of Chemistry, BUT, Institute of Food Science and Biotechnology 

Purkynova 118, 612 00 Brno 

vespalcova@fch.vutbr.cz 

Sea buckthorn (Hippophae rhamnoides) is an undemanding bush giving small 

orange fruits. The bush comes from Asia but it has spread almost all over the world 

because it can be grown without special agrotechnical technology. 

Sea buckthorn contains lots of substances beneficial to human health, particularly 

vitamin C in amount larger than in other kinds of fruit. Among other substances, 

also carotenoids, flavonoids, essential unsatured fatty acids and phytosterols 

should be mentioned. Today, sea buckthorn extract is added not only in homeopathic 

remedies and food supplements, but also in syrups, jams, sweets, oils, face 

creams, shampoos, soaps, teas and many other products. 

The aim of presented work was the finding out the sea buckthorn cultivar with 

the highest content of vitamin C and the highest antioxidative capacity among 15 

cultivars, which were growed by Faculty of Horticulture of Mendel University 

of Agriculture and Forestry in Brno. Two methods have been applied for the determination 

of the vitamin C content in the sea buckthorn juice: HPLC and the direct 

coulometric determination of the vitamin C. Total antioxidative capacity of the sea 

buckthorn juice was measured by the EPR. The analyses indicate that the cultivars 

“Velkoosecký” and “Botaničeskaja” seem to be optimal as far as its vitamin C content 

and the antioxidative capacity is concerned. 

Keywords: sea buckthorn, vitamin C, antioxidative capacity 

2.12


Posters 

2-P106 Arsenic Speciation in Fish Samples Using High 

Performance Liquid Chromatography Coupled 

with Hydride Generation Atomic Fluorescence 

Spectrometry 

2.12 

Eva Vitoulová, Blanka Macharáčková, Miroslav Fišera, Olga Čelechovská 

Brno University of Technology, Institute of Food Chemistry and Biotechnologies 


vitoulova@fch.vutbr.cz 

Arsenic is well known as a toxic element that occurs in foodstuffs in several 

forms. These forms have for humans different toxicity, therefore is total amount of 

arsenic in the foodstuffs insufficient information. The aim of the present work is speciation 

of arsenic in fish samples. Speciation method included coupling of separation 

by high performance liquid chromatography and detection by hydride generation 

atomic fluorescence spectrometry. Fish samples were extracted using five different 

extraction mixtures and their efficiency was compared. Obtained results showed 

that in all analysed samples extractable arsenic was present in the form of nontoxic 

arsenobetaine. 

Keywords: arsenic, speciation, fish


Posters 

2-P107 Comparison of Aroma Profiles of Several Types 

of Dark Chocolate 

Eva Vítová, Blanka Loupancová, Hana Štoudková, Jana Zemanová, 

Libor Babák, Ivana Macků 



evavitova@post.cz 

Chocolate is one of the most popular foods all over the world. This may be due 

to the „feel good“ effect that many people experience after indulging. Chocolate’s 

unique sensory characteristics, in particular flavour and texture, and some internal 

physiological factors are the cause of chocolate popularity. 

Flavour is one of the most important properties of chocolate and is the subject 

of extensive research. It is very complex and is determined by both the cocoa plant 

variety and the technology process. Its precursors are developed during fermentation 

and drying of cocoa beans and develop into cocoa specific aroma through Maillard 

reaction during roasting. All of the precursors interact to produce cocoa flavour 

components, such as alcohols, ethers, hydrocarbons, furans, thiazoles, pyridines, 

acids, esters, aldehydes, imines, amines, oxazoles, pyrazines and pyrroles. To date, 

descriptive studies have identified more than 600 volatile compounds in cocoa and 

chocolate. But it is very difficult to asses which components are really important for 

flavour and which are not. 

The aim of this work was to compare aroma profiles of three types of dark 

chocolate. Volatile aroma compounds were determined by the SPME method in 

conjunction with gas chromatography. 

Keywords: chocolate, aroma, SPME, GC 

2.12


Posters 

2-P108 Monitoring of Changes in Population of Yeasts 

During Fermentation of Grape Must. 

2.12 

Dana Vránová,Michaela Zdeňková, Renata Vadkertiová 


Purkyňova 118, Brno, Czech republic 

vranova@fch.vutbr.cz 

The fermentation of grape must into wine is a complex microbiological process 

characterized by the presence of a large number of different microorganisms. 

The composition of yeasts population on grape berries and leaves plays an important 

role in wine fermentations, as different genera, species and strains with their 

metabolic activity influence the sensory quality and organoleptic characteristics of 

wine. 

Yeasts associated with grape or wine ecosystem are usually classified in 15 

different genera: Brettanomyces/Dekkera, Candida, Cryptococcus, Debaryomyces, 

Hanseniaspora/Kloeckera. Kluyveromyces, Pichia, Metschnikowia, Rhodotorula, 

Saccharomyces, Saccharomycodes, Schizosaccharomyces, Zygosaccharomyses. 

The work monitors the changes in yeast population during spontaneous alcoholic 

fermentation of cider of Veltlin green cultivar. We isolated yeasts from vineyard 

ecosystem such as soil, vine leaves and grapes. Identification of the individual 

strains was carried out using molecular biological method of PCR-.RFLP. 

Keywords: Yeasts, PCR, taxonomy, wine fermentation


Posters 

2P109 Study of Stability of Cleansers Based on Active 

Chlorine 

J. Zemanova, E. Vitova, M. Dankova, M. Popelkova 

Department of Food Chemistry and Biotechnologies, 


Purkynova 118, CZ61200 Brno, Czech Republic 

zemanova@fch.vutbr.cz 

This work deals with following decrease of active chlorine in natrium hypochlorite 

in mixtures of cleansers, where natrium hypochlorite has disinfection effect. 

Hypochlorite belongs between oxygenous chlorine compounds that decompose 

slowly. Slow decomposition entails step by step decreasing oxidizing ability of 

hypochlorite. It is possible to slow down instability of hypochlorite by removing of 

causes of decomposition or by adition of stability additives. 

There was immixtured two sorts of stability substances into two types of samples 

and in final appreciation the effect of checking stabilization was recognitioned. 

The studying of content of active chlorine in samples was provided by iodometric 

titration. 

Lastly microbiology tests on disinfection effect of rest quantity of active chlorine 

in samples was effected. There was all results statistically evaluationed. 

Keywords: hypochlorite, stability, cleansers 

2.12


Posters 

2-P110 Vegetable Oils for Cosmetics – Evaluation 

of their Effects on Human Skin 

2.1 0 

J. Zemanova, H. Stoudkova, E. Vitova 

Department of Food Chemistry and Biotechnologies, Faculty of Chemistry, 


Purkynova 118, CZ61200 Brno, Czech Republic 

zemanova@fch.vutbr.cz 

The aim of this work was to focus on some vegetable oils commonly used in 

cosmetics, review their characters and to evaluate their effects on human skin by 

measuring some skin parameters. 

For these experiments there were choosed macadamia oil, castor oil, sunflower 

oil, olive oil, lineseed oil, etc. 

The skin parameters were assessed after applications of selected vegetable 

oils on human skin by measuring with diagnostic instrument MPA5 (Courage and 

Kazaka, Germany). The experiments proceeded in given intervals and they were 

statistically processed. 

Keywords: vegetable oils, human skin

Section 3: 

Chemistry of Inorganic 

Materials 

.1

Lectures 

.

Section 3 – Chemistry of Inorganic Materials 

Lectures 

3-L01 Non -Traditional Binders and Composites Tested 

at the Student Laboratories of the Iinstitute of 

Materials Chemistry, Faculty of Chemistry, Brno 

University of Technology 

Jiří Brandštetr, Jaromír Havlica, Tomáš Opravil, František Šoukal 



brandstetr@fch.vutbr.cz 

Recently, the effort to increase the productivity of materials results a.o. in the 

utilization of different non-traditional cements and composites based preferably on 

mineral “wastes” – secondary industrial products, which saves natural mineral resources 

and decreases the amount of discharged materials on the dumping sites as 

well as the CO2 emissions, exhibiting thus economical and ecological effect. Novel 

chemical and mineral admixtures enable the preparation of “tailored” material of 

demanded special properties. Very often, lack of information and/or unstable quality 

of secondary materials is the real reason of their insufficient use. Therefore, better 

publicity, adequate education at schools of all types, meetings and conferences 

can play very important role. At the University of Technology in Brno, the students 

in their diploma and postdoctoral theses solve in the laboratories many of these 

problems mostly from chemical point of view with the final objective to enlarge the 

assortment of binders and mineral composites. 

Keywords: materials productivity, mineral wastes, alkali activation, 

geopolymers, novel composites, binders, concrete, admixtures 

.


Lectures 

3-L02 Influence of Phosphate Content on Formation of 

Dicalciumsilicate Modifications 

. 

Jaromír Havlica, Teodor Staněk, Jan Baráček, Petr Ptáček, František Šoukal 


Purkyňova118, 61200 Brno 

havlica@fch.vutbr.cz 

Cement rotary kilns with temperatures about 1450°C are often used for disposal 

of risk compounds with organic nature. The combination of organic and inorganic 

byproducts contribute contemporary to changes of phase composition product. 

Utilization of meat and bone meal is one of ecologically attractive activity, where 

both effects may be considered as significant importance besides disposal also the 

advantageous formation of low energy cements. 

The incorporation of phosphates content in Portland cement clinker is limited 

because, increasing presence of phosphate ions in system Ca2SiO4- Ca3SiO3 results 

both in suppression of tricalcium silicate (alite) content and preference in formation 

of required beta form of dicalcium silicate (belite) in manufactured product. 

On the other side both phenomena are necessary conditions for processing of belite 

cement, where beta form of dicalcium silicate and absence of high temperature tricalcium 

silicate are favorable for reducing of calcium oxide content in cement. In 

next the lowering of lime stone consumption is important for reducing of monitored 

non required compound - carbon dioxide. Consequence of elimination of alite and 

followed temperature reduction may enable probably utilization of calcium oxide 

directly in cement without risk of delayed hydration processes. 

Keywords: meat and bone meal, calcium phosphate, calcium silicate, 

belite cement


Lectures 

3-L03 The Efficacy of Deacidification by Aerosols of 

MgO and Dolomite Microparticles 

S. Holubková, M. Jablonský, M. Bajziková, J. Hanus, J. Rychlý, V. Bukovský, 

S. Katuscak 


Faculty of Chemical and Food Technology, 

Slovak University of Technology in Bratislava, 

Radlinskeho 9, SK-812 37 Bratislava, Slovak Republic 

silvia.holubkova@stuba.sk 

The most environmentally friendly technological platform (TP) for the deacidification 

of books is air or its mixtures with water. This ecological carrier has been 

used to carry deacidifying particles, such as MgO and dolomite (CaCO 3 .MgCO 3 ) 

dispersed in the air (SoBu process), MgO and CaCO 3 dispersed in mixture of air 

and water vapor (Libertec). The MgO has also been used in a less eco-perspective 

perfluorinated carriers, non-ozone depleting conservation TP although possesing 

global warming potential which is 320 - 8400 higher than CO 2 (Bookkeeper). The 

efficacy of the air-water- MgO–dolomite TP has been studied and compared with 

the other TPs. The wood-containing paper test books were deacidified in SoBu industrial 

plant, air-conditioned at the relative humidity RH = 50 %, and aged in a sealed 

bags at 98±2°C. The kinetics of folding endurance (ω), tensile strength (l t ) were 

measured and the indexes of relative increase of the lifetime (S τω ), the folding endurance 

(S ω,t ) and the tensile strength (S lt,t ) were calculated. The process was evaluated 

through multifactorial evaluation according to requirements of the Consortium 

Kniha SK (CK SK ) and the Library of Congress (LOC). The air-water-MgO-dolomite TP 

deacidification fulfills both the CK SK and LOC requirements for the lifetime increase 

(S τω =4,7), improves the mechanical permanence sufficiently (S ω,15 =33,6; S lt,15 =1,24). 

Keywords: deacidification, efficacy 

.


Lectures 

3-L04 Distribution of Heavy Metals in the Products of 

Magnetic Separation of Siderite Ore From Nižná 

Slaná 

. 

Slavomír Hredzák, Michal Lovás, Štefan Jakabský, Dana Gešperová 



hredzak@saske.sk 

The contribution deals with the dry high intensity magnetic separation (HIMS) 

of siderite ore fines, namely grain size of 0–4 mm at four values of magnetic induction. 

The distribution of utility and waste components, such as Fe, Mn, SiO 2 , Al etc. 

into separation products have been observed. An attention was also paid to heavy 

metals, namely Cu, Zn, Pb, Ni, As and Hg. 

Naturally, an increasing of magnetic induction resulted in enhancement of 

mass yield into magnetic product from 33 to 88 %. It was proven, that product with 

content of 29–32 % Fe and 5–9 % of SiO 2 can be won in comparison with 27–28 % Fe 

and 11–12 % SiO 2 in the feed. As to non-magnetic product content of Fe decreases 

from 25 to 12 %, conversely, content of SiO 2 enhances from 14 to 39 %. 

The recovery of Fe and Mn into magnetic product gradually increases from 36 

to 95 % and in the case of SiO 2 , it is from 15 to 63 %. The content of heavy metals 

in the feed attains the values: 100–160 ppm of Cu, 90–92 ppm of Zn, 11–14 ppm of 

Pb, 19–23 ppm of Ni, 580–670 ppm of As and 1.3–1.6 ppm of Hg. Separation tests 

showed that recovery of heavy metals into magnetic product increases with growing 

value of magnetic field induction. It indicates fine intergrowth of heavy metals 

bearing phases, namely sulphides with the main utility minerals – siderite and ankerite. 

Keywords: magnetic separation, distribution of ore components


Lectures 

3-L05 Magnetically Modified Bentonite and Study of 

its Improved Sorption Properties 

Z. Orolínová, A. Mockovčiaková 



orolinova@saske.sk 

It is well known, that clay minerals and iron oxide particles are good sorbents 

of heavy metals, therefore it could be interesting to combine the adsorption properties 

of bentonite with the magnetic properties of iron oxides. In this work the 

natural clay rock bentonite was modified by the magnetic particles with the aim to 

enhance its sorption properties. The bentonite/iron oxide composites were prepared 

in different weight ratios at two selected temperatures 20 and 85 °C and characterized 

by powder X-ray diffraction (XRD) analysis and Mössbauer spectroscopy. The 

real weight ratios of clay/iron oxide composites were determined by magnetization 

measurements. The morphologies of the investigated samples were observed 

by scanning electron microscopy (SEM). The surface and pore changes of natural 

bentonite after magnetic modification was studied by nitrogen adsorption measurements. 

The sorption properties of natural bentonite and composite materials obtained 

from this study were compared. 

Keywords: bentonite, iron oxide particles, Mössbauer spectroscopy, 

surface and pore properties 

.


Lectures 

3-L06 Reductive Dissolution of Ferric Sulfate and 

Ferric Iron Oxihydroxides by Acidophilic 

Heterotrophic Bacteria 

.10 

Zuzana Pállová, Daniel Kupka, Alexandra Vašková, Zuzana Orolínová, 

Vladimír Šepelák. 


Watsonova 45, 043 53 Košice, Slovak Republic, 

pallova@saske.sk 

Reductive dissolution of synthetic iron(III)-oxides and oxi-hydroxides was carried 

out under acidic conditions in the presence of acidophilic heterotrophic ironreducing 

bacterium Acidiphilium SJH. Five synthetic iron-minerals; α-FeO(OH) 

(goethite), γ-FeO(OH) (lepidocrocite), α-Fe 2 O 3 (hematite), γ-Fe 2 O 3 (maghemite) and 

Fe 3 O 4 (magnetite) were prepared by either oxidative hydrolysis from solutions containing 

Fe 2+ or by hydrolysis from ferric iron solutions. The purity of “as prepared” 

mineral phases was confirmed by XRD analysis and Mössbauer spectroscopy. Ferric 

ions from the poorly soluble minerals served as sole electron acceptor for bacterial 

respiration. Bacteria reduced ferric iron to ferrous iron using D-galactose as the 

electron donor. The abiotic rates of iron extraction correlate with the solubility of 

the minerals. The specific initial rates of iron dissolution (μg m 2 h -1 ) normalized to 

biomass concentration related to absorbance unit OD 600 = 1.0 were in the range of 

0.53 to 26.46 μg m 2 h -1 and were in the order magnetite >lepidocrocite >maghemite > 

hematite> goethite. The rate of bacterial reduction of soluble Fe 3+ was by far higher 

than the rates of solid ferric iron compounds. Bacterial transformation of ferric to 

ferrous iron and vice versa takes place in many important environmental processes 

and could be used in many mineral processing.. 

Keywords: Iron oxides, reductive dissolution, Acidiphilium


Lectures 

3-L07 The Effect of Hydrogen on Combustion of 

Natural Gas 

Ondřej Prokeš, Daniel Tenkrát, Aleš Doucek 

Ústav plynárenství, koksochemie a ochrany ovzduší; VŠCHT Praha 

Technická 5; 166 28 Praha 6 

prokeso@vscht.cz 

Hydrogen is regarded as a fuel of choice for 21 st century. A lot of governments 

expect that the hydrogen takes up the leading role in the future energetic concepts 

of many industrial countries. The pure combustion of hydrogen produces extremely 

low or zero emissions. Needless to say that only water is raised during the hydrogen 

combustion with oxygen. If an air is used, moderate among of nitrogen oxides 

is possible to detect in exhaust gases. No doubt, it is evidently that the combustion 

of hydrogen is most ecological way how to produce an electricity, heat or cold in 

comparison with other fossil fuels. 

Hydrogen could be transported by all common ways – by ships, by railways or 

by trucks. Yet it is possible and efficient to use for distribution the accessible natural 

gas pipeline network. It means that both system natural gas and hydrogen could 

exist and operate together in the same pipeline. We can expect several problems 

during the transportation, distribution and utilization of hydrogen transported by 

the pipeline system which was built only for natural gas. Another aim of this lecture 

contains the analyses of the critical aspects by the combustion of the mixture hydrogen/natural 

gas with the different content of natural gas (from 3 up to 60 Vol. %). 

Keywords: hydrogen, natural gas, combustion 

.11


Lectures 

3-L08 The IR Spectroscopy Study of Particle Size 

Influence on Course of Kaolin Dehydroxylation 

.12 

Petr Ptáček, Dana Kubátová, Jaromír Havlica, František Šoukal, 

Tomáš Opravil 

Institute of Materials Chemistry, Faculty of Chemistry, Brno University of Technology 

Purkyňova 118, Brno, CZ-612 00, Czech Republic, 

ptacek@fch.vutbr.cz 

The particle size effect on dehydroxylation of washed kaolin was determined 

by mid-infrared spectroscopy via sets of isothermal – quasiisobaric experiments in 

temperature range from 150 to 650 °C. Correlations between primary spectroscopic 

variables, i.e. A amplitude), lmax (peak’s wavelength) and w (half-width of absorbtion 

band), temperature of dehydroxylation and particle size were investigated. 

Keywords: China clay, kaolinite dehydroxylation, meta-kaolinite


Lectures 

3-L09 Critical Particulates fineness of the Air 

Classification Processes 

Tomáš Svěrák, Ondřej Kozdas, Vítězslav Frank 

Brno University of Technology, Faculty of Chemistry, Institute of Materials Science, 

Purkynova 118, 612 00 Brno, Czech Republic; 

sverak@fch.vutbr.cz 

Air classifiers of particulate material operate on the basis of air-stream power 

and centrifugal forces equilibrium. This equilibrium takes an effect to separate particles 

which are dispersed and transported in the air stream lead to the working area 

of classifier rotors. The cylindrical rotor classifier with the radial flow of air stream 

is the basic air classifier configuration of very fine particles processes. This classifier 

configuration provides very reliable classifier processes with a sharp cut of grainsizing. 

But when the fineness of classified powdery material tends to the medium 

grain size d50 of about 1 micrometer and some adhesion forces take place there – the 

classifier system does not function any more. One of the reasons of this malfunction 

is the fact that the radial velocity of the “critical size” particles extremely decreases 

in the working area of classifier rotor and the concentration of the “critical size” particles 

fatally increases. The correct process of particle classification stops under the 

above process conditions. Authors discuss condition of the effect formation. 

Keywords: particulate solids processes, separation, air-classification 

.1


Lectures 

3-L10 Determination the Reaction Order for Fast 

Formation of CuS 2 O 3 

.1 

Mihaela Ligia Ungureşan, Francisc Vasile Dulp 

Technical University of Cluj-Napoca, Chemical Department, 

103-105 Muncii Bvd., 400641, Cluj-Napoca, Romania 

Mihaela.Unguresan@chem.utcluj.ro 

The kinetics of the redox reaction between copper ions and thiosulfate ions, 

respectively the formation of the intermediate complex, CuS 2 O 3 , are investigated. 

The reaction order, with respect to the intermediate complex depends upon the concentrations 

of the reactants. The kinetics of fading away of the color of this complex 

has been traced spectrophotometrically with a stopped-flow apparatus. The experimental 

reaction order is consistent with the concentration of the reactants. 

Keywords: Preequilibrium Kinetics, Fast Redox Reaction, 

Intermediate Complex CuS 2 O 3 .


Lectures 

3-L11 Ceramic Petrography of the Loštice Pottery 

Dalibor Všianský, Miroslava Gregerová, Martin Hložek, 

Ústav geologických věd, PřF MU 

Kotlářská 2, 61137 Brno, 

mirka@sci.muni.cz 

The paper summarizes the results of micropetrographic, geochemical and experimental 

examination of the mediaeval pottery from Loštice. The burning temperature 

and the affect of garnets on the formation of “blebs” were experimentally 

tested. A clay nodule found during archaeological works was used for a part of 

tumbler replicas’ production as a plastic raw material. Another part of replicas was 

made of local loess. (At the temperature higher than 1200 °C, the replicas made of 

loess collapsed.) The results showed that some of the pottery groups were made of 

the same, or very similar, plastic raw material with petrographically different nonplastics. 

The ceramic tumblers of the (010) and (020) groups were made of clay soil 

with the addition of almandine garnet as non-plastics. The “blebs” form by thermal 

decomposition of almandine. Cordierite and possibly also sekaninaite and ossumilite 

crystallize from the melt. The experiments verified that the burning temperature 

had reached 1200–1250 °C. 

Keywords: ceramic, petrography, pottery 

.1

Posters 

.1

3-P01 Optimalization of Lead Sulphide 

Mechanochemical Synthesis 

M. Achimovičová, A. Mockovčiaková, E. Dutková 



achimovic@saske.sk 


Posters 

The mechanochemical synthesis of PbS was performed by high energy milling 

of lead acetate and sodium sulphide in a planetary ball mill. Two preliminary experiments 

on using the process control agent-stearic acid were realized and the results 

concerning the particles size distribution and specific surface area were encouraging 

– thus a method of statistical factorial design – 1 st order model fitting 2 3 , with 

3 examined factors was used: the mean particle size as the measured response and 

time of mechanochemical synthesis-x 1 , revolutions of mill-x 2 and weight of stearic 

acid-x 3 – as independent factors. Basing on the design matrix a mathematical function 

Y = 1.91 – 0.12 x 1 – 0.47 x 2 + 0.12 x 1 x 3 – 0.43 x 2 x 3 , was obtained. 

It resulted from this function that the mean particle size of synthesized PbS 

nanoparticles was most influenced by revolutions of mill, less influenced by time 

of mechanochemical synthesis and the weight of stearic acid did not influence the 

mean particle size of synthesized PbS particles basically. 

X-ray diffraction analysis, particle size distribution analysis, specific surface 

area measurement and scanning electron microscopy were used for a characterization 

of microstructure and properties of synthesized PbS. UV-VIS spectroscopy was 

used for determination of size of the smallest PbS particles obtained by mechanochemical 

synthesis. 

Keywords: PbS nanoparticles, Mechanochemical synthesis 

.1


Posters 

3-P02 Synthesis of Doped Lanthanum Ferrite 

Perovskites 

.20 

Eva Bartoníčková, Jaroslav Cihlář 

Department of Physical and Applied Chemistry, Faculty of Chemistry, 



bartonickova@fme.vutbr.cz 

La 1-x Sr x Fe 1-y Ti y O 3-d where x = 0.8 and 0.8 ≥ y ≤ 0.2 perovskite systems were prepared 

via several methods. Both novel methods spray pyrolysis and glycine/nitrate 

combustion synthesis were compared with conventional solid state reaction with 

mechanical activation. 

The „wet chemical routes“of preparation proceeded from aqueous solution of 

nitrates of appropriate metals. As precursors of conventional route the homogenous 

mixtures of appropriate metal oxides were used. The properties of prepared ceramic 

powders were studied by XRD, chemical analysis, SEM, particle size determination, 

BET method, SEM. The comparison of products properties of prepared ceramic 

powders shows that the novel methods lead to improved final properties then the 

conventional technique. Nanostructured phase pure doped lanthanum ferrites with 

high specific surface area were prepared by spray pyrolysis. 

Keywords: lanthanum ferrite, solid state reaction


Posters 

3-P03 Effect of Heat Treatment on the Al + Si 

Diffusion Coatings on Ni-Based Alloys 

Ladislav Čelko, Lenka Klakurková 

Brno University of Technology, Faculty of Mechanical Engineering 

Technická 2896/2, 616 69 Brno, Czech Republic 

ycelko00@stud.fme.vutbr.cz 

The nickel based alloys surface structure stability are recently ensured by use 

of many types of protective coatings. One of this types of coatings are diffusion 

ones. These coatings are based on saturation of Ni-based substrates with elements 

Al, Cr, Si or their combinations. This can lead to an improvement of the oxidation, 

corrosion and wear resistance of nickel based alloys surfaces. 

This paper deals with preparation of Al+Si diffusion coatings on the nickel 

based substrates from slurries annealed at 1000°C. The phase transformations and 

element interactions were studied. As a substrate, Ni of commercial purity, Ni20Cr 

and Inconel 713 LC alloys were used. During annealing, the elements from coating 

interact with the substrate while forming intermetallic protective layer and an interaction 

zone between the protective layer and substrate. 

For this study the light microscopy, scanning electron microscopy, energy dispersive 

microanalyses, microhardness measurements and image analysis methods 

were used. 

Keywords: Diffusion coatings; Aluminides NiAl, Ni3Al; Diffusion; 

Heat treatment; EDS analyses 

.21


Posters 

3-P04 Chemical Analysis of Al and Si Surface Layers on 

Steels 

.22 

Pavel Doležal, Ladislav Čelko, Aneta Němcová, Lenka Klakurková 



pavdol@email.cz 

Properties of the metallic materials working at higher temperatures are strongly 

dependent on degradation processes imposed. Among the most important degradation 

processes belong: high temperature oxidation, corrosion and erosion. Surface 

modifications research and development currently takes place in order to improve 

properties and usability of these materials. 

One of many possibilities to ensure an improvement of the mettalic materials’ 

surface properties is use of the diffusion coatings. These are based on substrate 

surface saturation with Al, Cr, Si and their combinations. These elements are able to 

create compact protection intermetallic layer on the metallic substrate. 

This paper deals with phase transformations analysis, study of interaction of 

several elements between coating and substrate, formation of diffusion coating under 

different high temperature conditions. For evaluation and determination of diffusion 

coatings transformations the light microscopy, scanning electron microscopy, 

energy dispersive microanalyses, glow discharge optical emission spectrometry 

and image analyses methods were used. 

Keywords: Diffusion; Aluminides, Miscelaneous; Protective layers; 

Slurry deposition; Chemical analyses


Posters 

3-P05 Synthesis of Belite Cement from Coal Fly Ash 

Ivana Fiľková, Nadežda Števulová 

Technical University of Kosice, Civil Engineering Faculty 

Vysokoškolská 4, 042 00 Košice, Slovakia 

Ivana.Filkova@tuke.sk 

The Portland cement is one of the most consumption materials in the world. 

The production of this kind of cement is material and energetic demanding process 

and for that reason there is a growing interest in synthesis of belite cement motivated 

by economical implications and environmental concerns. The replacement of 

Portland cement by belite cement presents many positive aspects, such as energy 

and raw materials savings. 

This kind of cement has high flow ability, high strength, good durability and 

super low heat of hydration. Belite cement is ideal cementitious material realizing 

concrete high performance and especially suitable for mass concrete construction. 

In this paper, the results of hydrothermal-calcination route of belite cement 

preparing from mixture consisting of coal fly ash and CaO addition (CaO/SiO 2 = 2) at 

reaction parameters optimization are presented. 

Keywords: belite cement, belite, coal fly ash 

.2


Posters 

3-P06 Microbiologically influenced corrosion of 

concrete 

.2 

Vlasta Harbuľáková, Adriana Eštoková, Nadežda Števulová, 

Alena Luptáková 

Department of Material and Environmenal Engineering, Faculty of Civil Engineering, 

Technical University of Košice, 

Vysokoškolská 4, Košice, Slovakia 

vlasta.harbulakova@tuke.sk 

Microbially influenced corrosion studies (MIC) microbial activity impact on 

the durability of concrete materials. The role of microorganisms in concrete corrosion 

processes has been linked to the generation of sulfuric acid and nitric acids by 

sulfate- reducing bacteria (SRB), sulfur oxidizing bacteria and nitrifying bacteria. A 

most unfriendly act played by microorganisms is to colonize biofilms and to utilize 

chemical reactions to degenerate the structural materials. The biogenic acid cause 

dissolution of calcium containing minerals from the concrete matrices. 

The various concrete samples MIC was studied under model conditions by 

simulation of a sulfuretum. The Acidithiobacillus thiooxidans, Acidithiobacillus 

ferooxidans and sulfate - reducing bacteria SRB bacteria under optimal growth 

conditions were used. Experiments took place under anaerobic conditions during 

80 days at pH 7.5. The application of individual bacterial culture on the concrete 

samples this simulation of sulfuretum led to a greater concrete surface deterioration. 

The change of the concrete samples weight and change of the pH of the liquid 

phase was evaluated. The significant loss of concrete sample weight was observed. 

The concentration of calcium and silicium in the solution was determinated by AAS 

method. The high concentration of calcium ions in comparison with silicium ion 

released from concrete samples were found out in the solution. 

Keywords: biocorrosion, concrete, MIC


Posters 

3-P07 Influence of Salts Admixtures on Gelation 

Process of Silica 

Jaromír Havlica, Petr Ptáček, František Šoukal, Martin Zmrzlý 



havlica@fch.vutbr.cz 

Dissolution of salts in electrolyte in coexistence with silica sol causes decreasing/increasing 

of pH value. During addition of various inorganic or organic compounds 

the stability of sol/gel is changed and obtained gel product is characterized 

by modification of gelation kinetics (ageing), mechanism of process. 

Ageing of silica sol based solution (TOSIL) with sodium hydroxide, sodium acetate 

trihydrate, calcium chloride hexahydrate, sodium chloride, calcium sulphate 

dihydrate, sodium sulphate, calcium nitrate tetrahydrate, sodium nitrate, and sodium 

oxalate resp. has been observed and analyzed. Evaluation of gel formation 

parameters may be used for design of optimum condition for control of properties 

of resulting gel. 

Keywords: silica sol, gelation, ageing 

.2


Posters 

3-P08 Thaumasite preparation 

.2 

Markéta Hermanová, Vítězslav Frank 


Purkyňova 118,612 00 Brno, Czech Republic 

hermanova@fch.vutbr.cz 

Presented work deals with preparation of thaumasite from different starting raw 

materials. The influence of physical conditions (like temperature, time, humidity) 

and storage conditions on thaumasite formation was studied. The phase composition 

of prepared samples was analyzed by XRD. The morphology of prepared thaumasite 

was studied by scanning electron microscope (SEM). It was showed, that thaumasite 

is formed at temperatures between 0 and 5 °C and under high humidity. Under these 

conditions, thaumasite was found already after two months. 

Keywords: thaumasite, thaumasite preparation


Posters 

3-P09 An Application of Al-Si Layer on Nickel-Based 

Superalloys and their Analysis 

Martin Juliš, Simona Pospíšilová 

BUT, Faculty of Mechanical Engineering 

Technická 2896/2, 616 69 Brno 

pospisilova@fme.vutbr.cz 

Production of new generation of aircraft gas turbine engines is based on designing 

new constructions and materials, which make it possible to operate under 

still more severe conditions. The key assembly of the engine is its turbine, whose 

materials and design determine the tolerable gas temperature. Increased inlet gas 

temperatures resulted in the shortening of the service life of the blades protected 

with diffusion coatings. New principles of coating deposition opened up new possibilities 

for purposive improvement of coating compositions and variation of their 

properties. 

This paper is focused on microstructure and chemical analysis of protective 

layers created by co-deposition of Al and Si on nickel-base superalloys INCO 713 LC 

and INCO 738 LC after thermal and thermal-stress exposition and on microstructure 

and properties of basic materials (substrates). The contribution also shows creep 

and low fatique tests results for both superalloys with and without a protective 

layer. 

Keywords: Al-Si layer, nickel-based superalloy 

.2


Posters 

3-P10 Alkaline Modified Coal Fly Ash as an Addition 

to Concrete 

.2 

Jozef Junák, Nadežda Števulová 


Vysokoškolská 4, 04200 Košice, Slovakia 

jozef.junak@tuke.sk 

Utilization of coal fly ash in concrete production is beneficial from the point 

of view of resource conservation, energy saving and CO 2 emission reduction and it 

contributes to solution of problems with the disposal of this waste kind. 

In this paper, the results of coal fly ash modification for its using in material and 

environmental engineering are presented. Alkaline activation influence of coal fly ash 

and its mixtures with Portland cement in an aqueous NaOH solution during hydrothermal 

treatment as well as the influence of cement replacement by fly ash on zeolite phase 

formation and compressive strength development of 28 and 90 days hardened coal fly 

ash/cement composites was investigated. Study of zeolite formation in composites was 

carried out by XRD analysis. Compressive strengths of hardened composites based on 

alkaline activated coal fly ash/cement mixture and on cement replacement by starting 

and zeolitized coal fly ash were compared to referential composite without coal fly ash 

and evaluated according to the STN EN 450 by the value of relative strength. The results 

of relative compressive strengths of studied composites confirm that the formation of 

zeolitic structures during hardening of mixtures plays an important role at compaction 

of system. 

Keywords: coal fly ash, alkaline treatment, zeolite


Posters 

3-P11 Elements Interaction Analyses During 

Preparation of Al-Al 3 Ni Eutectic Composites 

Lenka Klakurková, Ladislav Čelko, Pavel Doležal, Ondřej Man, 

Karel Němec 



klakurkova@fme.vutbr.cz 

Understanding of equilibrium and non-equilibrium thermodynamic phase 

transformations is one of the basic condition for design of intermetallic particle 

strenghted composites. The Al-Ni binary system is already used for high temperature 

applications (Nickel based superalloys and high temperature protective coatings) 

where the Ni3Al and NiAl intermetallics widely appear. But this system contains 

another intermetallic compounds Al3Ni, Al3Ni2, Al3Ni5 which are neglected 

in high temperature studies. 

These intermetallic compounds can be employed as strenghtening phases in 

Al-based composites. The advantages of these materials are low density and relatively 

good chemical resistance. They can reach attractive properties in combination 

with less known Al-Ni intermetallic phases strenghtening. 

This paper is focused on the developement of Al-based composites prepared 

by HVOF nickel powder deposition on Al substrate. Use of this method alone is 

not sufficient without a post-deposition heat treatment at temperatures close to the 

melting point of pure Al. The temperatures for difusion and formation of this intermetallic 

phases are in the range from 600°C to the 630°C. 

For this study the light microscopy, scanning electron microscopy, energy dispersive 

microanalyses and image analyses were used. 

Keywords: Al-Al3Ni composites; Diffusion; EDS analyses 

.2


Posters 

3-P12 Magnetic Properties of Waste Vitrified in 

Microwave Furnace 

. 0 

Milota Kováčová, Michal Lovás, Marek Matik, Vladimír Šepelák 


Watsonova 45, Košice, 043 53, Slovakia 

kovacova@saske.sk 

The paper deals with the magnetic properties of an iron-containig waste from 

the former nickel hydrometallurgy plant in Sereď (Slovakia). The waste, nickel 

leaching residue, was used as a model carrier of heavy metals (Co, Cu, Cd, Pb, Ni) 

from wastewater treatment, which was necessary to stabilize. Microwave vitrification 

was used for stabilization of heavy metals carriers. 

The magnetic properties were studied by magnetic susceptibility measurement. 

The dependence of magnetic susceptibility on Fe content hasn’t been proved. 

According to Mössbauer spectroscopy measurements, the magnetic properties depend 

on magnetic state, which is influenced by grain size of vitrified samples. 

Keywords: waste, microwave vitrification, magnetic susceptibility, 

Mössbauer spectroscopy


Posters 

3-P13 The use of Electrokinetic Potential Measurement 

for Examination of Kaolinite Decomposition 

Dana Kubátová, Petr Ptáček, Jaromír Havlica. 

Výzkumný ústav stavebních hmot, a.s. 

Hněvkovského 65, 617 00 Brno 

kubatovad@centrum.cz 

Kinetics and mechanism of metakaoline formation has been examined by electrokinetic 

potential measurement, TG-DTA and FT-IR analyses. The industrial kaoline 

used has been processed at the temperature range 105-800°C. The dehydroxylation 

of kaolinite (loss of OH groups) results in a surface change that leads to 

decreasing of system stability and increasing of electrokinetic potential, simultaneously. 

This increase occurs at the temperature range 400-550°C. 

Keywords: metakaoline, zeta potential 

. 1


Posters 

3-P14 Analysis and Interpretation of Height Alumina 

Refractories Corrosion by Alkali and Vanadium 

Oxides 

. 2 

Tomáš Opravil, Petr Ptáček, Jaromír Havlica,František Šoukal, 

Michal Vršecký 




The study provides an essential information about course of alkaline and vanadium 

corrosion process of shaped height alumina refractories. Samples taken from 

furnace lining of reactor on production of synthesis gas were analyzed by XRD, 

EDAX, FT-IR and REM for identification of corrosion products. 

Keywords: Refractories, Corrosion, Mullite


Posters 

3-P15 Measurment of Heat of Hydratation of Roman 

Cement 

Tomáš Opravil, Petr Ptáček, František Šoukal, Jaromír Havlica 

Institute of Material Chemistry, Faculty of Chemistry, Brno University of Technology 

Purkyňova 118, Brno CZ – 612 00, Czech Republic, 

opravil@fch.vutbr.cz 

The Roman cement (natural cement) is no longer available on the market and 

there is currently an absence of any broader information on its characteristics. The 

work is from this account devoted to the study of the final burnt materials with any 

composition on preliminary materials. The main idea of this work is find optimal 

and easy method for measurement of heat of hydratation. 

Keywords: Roman cement, heat of hydratatoin, hydraulic lime, 

inorganic binders, 

.


Posters 

3-P16 FT-IR Spectroscopic Study of Portland Cement 

Hydration 

. 

Tomáš Opravil, Petr Ptáček, Jaromír Havlica, František Šoukal 



opravil@fch.vutbr.cz 

Portland cement hydration process was studied by mid-IR spectroscopy. Infrared 

spectroscopy is suitable method for main hydration product identification and 

investigation of the reaction course. Furthermore spectrum contains number of additional 

information about interactions and processes in C-S-H gel phase. 

Keywords: Portland cement, hydration, C-S-H


Posters 

3-P17 Synthesis of Calcium, Magnesium and Zinc 

Negative Thermal Expansion LAS Ceramics 

Petr Ptáček, Helena Krejčová, Jaromír Havlica, František Šoukal, 





Lithium aluminosilicate ceramics (LAS) based on β-spodumene phase were 

synthesized via solid state sintering. The width of suitable temperature interval 

for sintering been improved by addition of calcium, magnesium and zinc. The stabilization 

of spodumene in solid solution leads to LAS ceramics thermal behavior 

changes. The DTA results show that the doped samples have been melted by congruent 

melting process. 

Keywords: LAS ceramics, Negative thermal expansion, Spodumene 

.


Posters 

3-P18 Catalytic Actitity of Titania-Kaoline Composite 

. 

Petr Ptáček, Michal Fiala, Jaromír Havlica , 

František Šoukal, Tomáš Opravil 




TiO 2 / Na-kaolinite composite were prepared via TiCl 4 hydrolysis in aqueous 

kaolin suspension. Effects of phyllosilicate support on the TiO 2 catalytic efficiency 

in temperature range from 100 to 400 °C was evaluated via ethanol vapor phase 

oxidation in flow reactor. 

Keywords: TiO 2 /Na-kaolinite composite, catalytic efficiency


Posters 

3-P19 Deposition of the TiO 2 on a Glass and Ceramics 

Surface 

Alena Rutteová, Jaromír Havlica, Petr Ptáček 

Institute of Materials Chemistry, Fakulty of Chemistry, Brno University of Technology 

Purkyňova 118, Brno, CZ-612 00, Czech Republic 

xcrutteova@fch.vutbr.cz 

TiO 2 has to be considered as one of the most important semiconductor photocatalyst. 

Imobilization of photocatalyst TiO 2 was made by precipitating from gas 

on to glass or ceramics support. The photocatalyst layer TiO 2 was evaluated by the 

optical microscopy. With using the methods of infrared spectroscopy, X-rays diffraction, 

differential thermal analysis and particulates size distribution samples of TiO 2 

dispersion in water and SiO 2 and TiO 2 hydrosol were analyzed. 

Keywords: Titanium dioxide, photocatalysis 

.


Posters 

3-P20 Composition Changes of Clinker with P 2 O 5 

Content 

. 

Theodor Staněk, Petr Sulovský, Lenka Tomancová 

Výzkumný ústav stavebních hmot, a.s., 

Hněvkovského 65, 617 00 Brno, 

stanek@vustah.cz 

The influence of P 2 O 5 on clinker phase composition was examined in laboratory 

conditions. P 2 O 5 affects the properties of clinker melt, it enters clinker minerals´ 

structures, influences clinker phase composition and, hence, affects the properties of 

cement. A high P 2 O 5 content appears in meat and bone meal that represents one of 

important secondary fuels nowadays. P 2 O 5 in a form of calcium phosphate or meat 

and bone ash in the amount of 5 wt % was added to cement-making raw meal prepared 

of common cement raw material. Further, experiments with pure components 

were carried on and the influence of P 2 O 5 on phase composition at the absence of one 

of the main clinker oxides (Fe 2 O 3 or Al 2 O 3 ) was examined. The phase composition of 

clinkers burned to equilibrium and non-equilibrium was quantified by optical point 

counting method. The entry of P 2 O 5 into clinker minerals´ structure was examined 

by electron microanalysis. At these laboratory experiments, the negative influence 

of P 2 O 5 was already observed at the content of 0.7 wt. % in clinker. The P 2 O 5 content 

of 4.5 wt. % totally blocked alite formation. A partial replacement of SiO 2 by P 2 O 5 

along with Al 2 O 3 in clinker silicates via so called berlinite substitution was revealed 

by electron microanalysis. Other experiments have shown that the Fe 2 O 3 absence 

eliminates the P 2 O 5 negative influence on the alite formation to some extent. 

Keywords: clinker, P 2 O 5


Posters 

3-P21 Spectroscopic Investigation of some 

Transitional Metals Complexes with Leucine as 

Ligand 

A. Stanila , A. Marcu, D. Rusu, M. Rusu 

Agricultural Sciences and Veterinary Medicine Univ. 

400018 Cluj-Napoca, Romania 

andreea s@yahoo.com 

The purpose of the study was to obtain neutral complexes of ML 2 ⋅nH 2 O type at 

pH = 8–10, in the presence of a strong basis (NaOH) . 

The [Cu(L) 2 ]⋅H 2 O (1), [Co(L) 2 ]⋅2H 2 O (2) and [Zn(L) 2 ]⋅H 2 O (3) complexes with 

leucine (L) as ligand, were synthesized and characterized by means of atomic absorption, 

elemental and thermal analyses, IR, UV-VIS and ESR spectroscopic investigations. 

The elemental analysis and atomic absorption spectroscopy measurements confirm 

the ration 1:2 metal ion: leucine composition for the synthesized complexes. 

The FT-IR data show that leucine is acting as a bidentate ligand with coordination 

involving the carboxylic oxygen and the nitrogen atom of the amino group. 

Spectral UV-VIS and EPR spectra at room temperature are typical for monomeric 

species, the pseudotetrahedral symmetry around the copper and zinc ions 

and the octahedral simmetry for the cobalt ion. 

Keywords: spectroscopy, leucine 

.


Posters 

3-P22 The Study of Compressive and Flexural Strength 

of High Performance Concrete (HPC) as the 

Function of its Composition 

. 0 

Pavel Šiler, Josef Krátký, Ondřej Veleba, Pavel Jedlička 

FCH VUT Brno 

Purkyňova 118, 61200, Brno, Czech republic 

siler@fch.vutbr.cz 

The work is aimed at the study of the compressive and flexural strength of 

high performance concrete (HPC) as the function of its composition. Constituents 

used for HPC preparation were portland cement, white cement, silica fume, finely 

ground granulated blast furnace slag, finely ground silica (SUK), bauxite and 

polycarboxylate superplasticizer. The influence of the HPC composition on hydration 

process was observed by means of isoperbolic calorimetry. The compressive 

strength of HPC prepared was in the range of 150–220 MPa. Flexural strength was 

in the range of 10 – 20 MPa. All specimens were prepared using standard mixer and 

wet cured for 28 days before the mechanical parameters were measured. 

Keywords: high performance concrete, isoperbolic calorimetery, 

compresive strength, flexural strength


Posters 

3-P23 Temperature and Moisture Effects on 

Macrodefct-Free Composite Structure and 

Properties 

František Šoukal, Jiří Másilko, Jaromír Havlica, Petr Ptáček, Tomáš Opravil 



soukal@fch.vutbr.cz 

Inorganic cement combined with water-soluble polymer after appropriate 

high-shear processing can result into the ultra-high-strength material called macrodefect-free 

composite. Macrodefect-free composites are sensitive to moisture due 

to water-soluble polymer content. A temperature and moisture effects during setting 

and aging of composite on its chemical and physical structure and its relevant 

properties were studied. 

Keywords: macrodefect-free, temperature, moisture 

. 1


Posters 

3-P24 Latex Modified Cement Composites: Effect of 

Polymer Type 

. 2 

František Šoukal, Václav Vinter, Jaromír Havlica, Petr Ptáček, 





The latex modified cements offers some improved or unique properties in the 

field of cemetitious materials. The type of polymer used within the latex significantly 

influences hydration mechanism and final properties. The paper discusses this 

effect in the terms of structure development and mechanical properties of resulting 

composite. 

Keywords: cement, latex, modification, polymer type


Posters 

3-P25 Influence of Conditions on Hydraulic Calcium 

Aluminate Phases Hydration Mechanisms 

František Šoukal, Jan Koplík, Jaromír Havlica, Vítězslav Frank, Petr Ptáček 




Nowadays literature does not clearly describes the hydration mechanisms of 

hydraulic calcium aluminate phases such as C 3 A, CA, CA 2 and C 12 A 7 in the terms 

of resulting hydrate and influence of hydration conditions. This paper deals with 

the effect of pH value and temperature on hydration mechanism of these hydraulic 

phases. 

Keywords: calcium aluminate, hydration mechanism 

.


Posters 

3-P26 The Study of Building Materials 

Biodeterioration by Inovatives Methods 

. 

Eva Terpáková 

Technical University Košice, Civil Engineering fakulty, 



eva.terpakova@tuke.sk 

Biodeterioration of building materials - stone, concrete, mortar, etc., can be 

caused by such microorganisms as bacteria, fungi, lichens, algae, and plants. Multidiscipline 

methods are available for the study of biodeterioration each other materials. 

Almost of them is bearing to biological part of problem e.g. identification of 

biodeteriorates, evaluating of birth biofilm or metabolites studies. 

This paper reports on the development of accelerated tests for evaluating microbial 

influenced degradation (MID) of concrete samples as model matrix one of 

pipe system material. Aggressive MID microorganisms can form a biofilm on the 

surface of model samples so that when nutrients are provided the microbes remain 

active. The changes of physico – chemical characteristics of model samples due to 

MID were studied by several analytical methods. Elemental mass loss data from 

exposed sample forms indicate the continued development and growth of microbes 

on the surface of samples. The differences in alkaline reaction confirmed the neutralisation 

process of cement stone due to MID. Calorimetric studies of MID processes 

are evaluated besides. 

Keywords: biodeterioration, MID, concrete, diagnostic methods


Posters 

3-P27 Milling of Olivine (Mg, Fe) 2 SiO 4 in High-Energy 

Mills by Wet and Dry Way 

Erika Turianicová, Peter Baláž 


Watsonova 45, 043 53 Košice 

turianicova@saske.sk 

The effect of grinding on the olivine (Mg, Fe) 2 SiO 4 is studied with great interest 

because it is a mineral possible to use as a suitable feedstock to carbon dioxide 

sequestration. 

This idea was originally proposed by Seifritz and first studied in more details 

by Lackner. The basic concept behind CO 2 mineral sequestration is to mimic natural 

weathering process in which Ca- or Mg- containing minerals are converted into Ca- 

or Mg- carbonates as exemplified by the following equation: 

(Ca, Mg)SiO 3 + CO 2 → (Ca, Mg)CO 3 +SiO 2 (1) 

The process of silicate minerals conversion according (1) is very slow (of thousands 

to millions of years). This is attribute to ambient conditions. Increasing the 

temperature and pressure of CO 2 is favoured over mineral carbonates at high temperatures. 

Mechanical activation by high-energy milling can results in major improvement 

of the reaction rate. 

The paper deal with high-energy milling of olivine sample, originated from 

the production plant at Ǻheim (Norway), in three mills. Physico-chemical changes 

induced by mechanical activation in a planetary mill, attritor and nutating mill are 

studied. By means of the grinding process it is possible to reduce particle size and to 

increase external surface area. The specific surface area was determined by the low 

temperature nitrogen adsorption method 

Keywords: olivíne, grinding 

.


Posters 

3-P28 Numerical Modelling and Simulation for 

Interaction between Cu2+ 2- and S O 2 3 

. 

Mihaela Ligia Ungureşan, Mihail Abrudean, Paula Raica, Eva Dulf, 

Tiberiu Coloşi 

Technical University of Cluj-Napoca, Chemical Department, 

103-105 Muncii Bvd., 400641, Cluj-Napoca, Romania 

Mihaela.Unguresan@chem.utcluj.ro 

The experimental reaction order is consistent with the mechanism involving in 

the first step complex’s fast formation and in the second, the reaction between two 

complex molecules. 

For family of curves y = y(t, p), experimentally rise up, y is the light intensity 

and it is considered as dependent on time t [ms] and concentration p[mol/l]. We are 

following the significant stages of identification – numerical modeling and simulation, 

in a usual systemic variant. 

The analogical model results from the reaction mechanism Cu2+ 2– with S O 2 3 

and through numerical simulation the following kinetics parameters can be obtained: 

the rate constants k 1 = 9.7; k -1 = 0.7 şi k 2 = 8222.5; molar absorption coefficient 

ε = 1489.7 M -1 cm -1 and the reaction order in report to reaction intermediate, at his 

decomposition, n = 2. The results run on the computer are being compared and 

interpreted in report to the experimental measurements. 

Keywords: rapid redox reaction, exponential functions, state variables, 

Taylor series, numerical integration.

3-P29 Bioactivity and Cytotoxicity 

of Fluorhydroxyapatite Ceramic 


Posters 

Martin Vitkovič, Maha Salih Mahammed Noaman and Martin Palou 

Slovak University of Technology, Faculty of Chemical and Food Technology 


martin.vitkovic@stuba.sk 

There is an increasing need for medical implants, due to an increasing aged 

population. Bone defects resulting from trauma, disease or developmental anomalies 

can substantially be improved by reconstructive surgery. 

Bone is essentially constituted of nanoscale inorganic materials and proteins. 

The inorganic materials are minerals which are structurally apatite-like, such as 

hydroxyapatite [HA, Ca 5 (PO 4 ) 3 OH], fluorapatite [FA, Ca 5 (PO 4 ) 3 F] and carbonateapatite. 

Therefore, composite fluorhydroxyapatite (FHA) materials have been developed 

by different synthesis routes and techniques in order to improve both bioactivity 

and mechanical properties of various orthopaedic prosthesis and dental 

implants. Besides bioactivity and mechanical properties, the non-cytotoxicity is one 

of the most important requirements for applications of these materials. 

The present work deals with the bioactivity and the cytotoxicity investigation 

of ceramic materials on the base of apatites with general formula Ca 5 (PO 4 ) 3 (OH) 1-x F x 

(x = 0, 0.5 and 1). 

Keywords: fluorhydroxyapatite, bioactivity, cytotoxicity 

.


Posters 

3-P30 Microwave-Hydrothermal Synthesis of 

Hydroxyapatite From CaSO 4 · 1/2H 2 O 

. 

P. Zamazalová , Ch. Damia, E. Champion, O. Gedeon 

Department of Glass and Ceramics, ICT Prague 

Technická 5, 166 28 Prague 6, Czech Republic 

Petra.Zamazalova@vscht.cz 

Dental caries is a world wide spread illness affecting inhabitants on every continent. 

Caries can be formed in micro-defects occurring on the surface of dental 

enamel as a result of demineralization process. In order to prevent caries formation 

in these defects a suitable material can be used to fill in present defects and cure 

them. As generally known, enamel can be chemically defined as non-stoicheiometric 

apatite (Ca 10-x (HPO 4 ) x (PO 4 ) 6-x (OH) 2-x · x H 2 O, 0


Posters 

3-P31 Conversion Coatings on Magnesium Alloys 

Martin Zmrzlý 

Faculty of Chemistry, Brno University ofTechnology 


zmrzly@fch.vutbr.cz 

Magnesium alloys are very perspective materials because of their low density 

and good mechanical properties. Main disadvantage of these alloys is their poor 

corrosion resistance. Moreover, one of the effective ways of their corrosion protection, 

the chromate coating, is nowadays not applicable since it is harmful to health 

and environment. So that new ways of corrosion protection have to be developed 

and adjusted for technological use. 

Keywords: Magnesium alloys, conversion coatings 

.

. 0

Section 4: 

Chemistry of Organic 

Materials 

.1

Lectures 

.

Section 4 – Chemistry of Organic Materials 

Lectures 

4-L01 Effect of Water Dissolution and Freeze- 

Drying on the Solid State NMR Sensitivity of a 

Heterogeneous Organic Mixture 

Pellegrino Conte, Anne E. Berns, 

Peter Burauel, Hans-Dieter Narres, Harry Veerecken 

Dipartimento di Ingegneria e Tecnologie Agro-Forestali, 

Università degli Studi di Palermo (Italy) 

v.le delle Scienze 13, ed. 4, 90128, Palermo – Italy 

pellegrino.conte@unipa.it 

The effect of the widely used freeze-drying storage technique on the sensitivity 

of CPMAS 13C NMR spectra of a heterogeneous mixture was investigated. The 

main factor controlling the CPMAS 13C-NMR sensitivity is the strength of the C-H 

dipolar interactions, which in turn is weakened by both rapid molecular motions 

and the absence of protons in the neighbourhood of carbon atoms. Changes in sensitivity 

of CPMAS 13C NMR spectroscopy were observed when large amounts of 

proton nuclei were supplied to natural organic matter (NOM) by water adsorption 

and when mutual spatial orientation of molecules in complex mixtures were modified 

during sample preparation. The procedures for sample preparation may affect 

CPMAS 13C NMR sensitivity, thereby providing undesirable results (i.e. either signal 

loss or quantitative overevaluation) when detailed information on complex mixtures 

are needed at a molecular level. Hence, a heterogeneous mixture was prepared 

by mixing equal amounts of four different known compounds. Part of the mixture 

was kept unchanged, whereas a second part was first suspended in water and then 

freeze-dried. The mixtures are then analysed by CPMAS-NMR. 

Keywords: Natural organic mixtures, CPMAS 13C-NMR 

.


Lectures 

4-L02 Aminoacid Profiles Monitoring for Diagnosis 

. 

Monica Culea, Andreea Iordache, Cornelia Mesaros 

Babes-Bolyai University, 

Str. Kogalniceanu, nr.1, 3400 Cluj-Napoca, 

mculea@phys.ubbcluj.ro 

A rapid GC/MS method for diagnosis of some neonatal diseases is described. 

Small volumes of 20 μl of plasma or blood spots were used for neonatal blood 

screening for diagnosis of phenylketonuria and other metabolic diseases. The blood 

samples were derivatized as trifluoroacetylbutyl esters and analyzed by gas chromatography 

coupled with mass spectrometry in the SIM mode. Regression curves 

for some standard amino acids are used for quantitative determination of valine, 

leucine, proline, phenylalanine and tyrosine. Samples were separated on a Rtx-5MS 

capillary column, 30 m x 0.25 mm, 0.25μm film thickness, using a temperature program 

from 50 °C(1min), then 20°C/min to 310 °C in the SIM mode. The following 

conditions were followed: transfer line temperature: 250oC, injector temperature: 

200 °C; ion source temperature 250 °C; Splitter: 10:1. Quantitative determination 

of the amino acids of interest was made in the SIM mode by selecting the highest 

ions of the mass spectrum and the m/z 183 for the internal standard, 15N-labelled 

isoleucine. 

Keywords: blood, diagnosis, amino acid, SIM-GC-MS


Lectures 

4-L03 Microwave-Assisted Extraction of Organic 

Compounds from the Brown Coal 

Silvia Čuvanová, Michal Lovás 


Watsonova 45, SK-043 53 Košice, Slovakia, 

cuvanova@saske.sk 

This article presents a new approach to evaluation of coal with implementation 

of microwave extraction of powders. It is know from literature that organic structure 

of coal is the base for preparation of organic materials. It has been tested the 

efficiency of different solvents for the extraction of polycyclic aromatic hydrocarbons 

in various organic solvents (dichloromethane, toluene, methanol) at the various 

conditions. The temperature of boiling at conditions of microwave heating in 

open loop system increased in comparison with conventional heating depending on 

type of solvent. MAE (microwave-assisted extraction) was also realised in pressure 

vessels. MAE and Soxhlet extraction of mechanically activated samples were compared. 

The extracts were tested by GC-MS analysis. 

The distribution of temperature in the extraction solvent was operated by modelling 

program Comsol, version 3.3. 

Keywords: brown coal, microwave-assisted extraction (MAE), 

organic compounds 

.


Lectures 

4-L04 Reactivity of N-NO 2 Bonds in Nitro Amides 

. 

Zdeněk Friedl, Svatopluk Zeman 


Purkyňova 118, CZ-61200 Brno, Czech Republic 

friedl@fch.vutbr.cz 

The homolytic dissociation of C-NO 2 , N-NO 2 or O-NO 2 represents the primary 

fission process of energetic materials under thermal, impact, shock and electric 

spark initiation stimuli. In the case of nitramines and nitro amides the N-NO 2 bond 

fission is mostly characterized by homolytic bond dissociation energies BDE(N- 

NO 2 ). The theoretical calculations of BDE energies are substantially influenced from 

inadequate treatment of electron correlation. Recently the alternative method was 

suggested to overcome this substantial drawback – the bond separation approach 

described by an isodesmic reaction. This type of virtual symmetrical chemical equilibria, 

characterized as bond disproportionation reactions, inherently cancel the 

electron correlation effects accompanying homolytic bond dissociation. 

The bond disproportionation energies DISP(N-NO 2 ) and bond dissociation 

energies BDE(N-NO 2 ) were evaluated for 10 nitro amides at ab initio DFT B3LYP/6- 

311+G(d,p) level. The DISP and BDE energies were correlated both with further 

theoretical molecular descriptors (total electronic charges of NO 2 groups) and main 

detonation parameters (detonation velocities D and heats of detonation Q real ) of nitro 

amides studied. 

Keywords: nitro amides, bond dissociation energies, DFT calculations


Lectures 

4-L05 XRD and FT-IR Study of Soot Obtained from 

Pyrolysis of Used Tires 

Slavomír Hredzák, Silvia Ivanová 



hredzak@saske.sk 

The paper deals with XRD and FT-IR study of soot specimens coming from 

a pilot pyrolytic plant for processing of crushed used tires in the Slovak Leaching 

Works – Chemistry, Inc. Hnúšťa. The specimens were prepared using a dry low 

intensity magnetic separation to remove the residues of thin steel wires and other 

Fe-bearing phases. 

The products of magnetic separation were subjected to XRD study. Two forms 

of graphite and lonsdaleite were found as main carbon phases. Moreover, an occurrence 

of amorphous phase, calcite, rankinite and magnetite was detected. 

Non-magnetic product was analysed using FT-IR. The FT-IR study of soot samples 

proved carbon bonds in alkanes, alkenes, alkines also in aromatic compounds. 

Slow bands of graphite, amorphous carbon and calcite were found. Carbon bonds 

with other components such as C–S, C–SO 2 –C, Si–O–Si, Zn–O was also identified, 

which corresponds with chemical analyses and XRD pattern. 

Finally, obtained results showed, that for removing of Fe-bearing components 

a low intensity magnetic separation is sufficient. Deferrized pyrolytic soot contains 

about 80 % C and such soot can be generally applied in production of lower quality 

rubber and/or as a filler to asphalt mixtures for pavement. 

Keywords: soot, magnetic separation, XRD, FT-IR 

.


Lectures 

4-L06 Determination of Yield and Chemical 

Composition of Soda-Anthraquinone 

Semichemical Pulp from Hardwood Mixture 

.10 

Michal Letko, Erika Nováková, Milan Vrška 



Radlinského 9, 812 37, Bratislava, Slovakia 

michal.letko@stuba.sk 

A lot of qualitative parameters of semichemical pulp are derived from yield. 

However, determination of yield is very difficult in this case. It is interesting from 

economical point of view as well. Yield is very often determined on the basis of functionalities 

and reaction conditions of process. 

This work is focused on determination of yield of semichemical pulp prepared 

by sulfur-free delignification process SAQ (NaOH/Na CO + anthraquinone) with 

2 3 

subsequent defibration of chips. Delignification of hardwood mixture with various 

content of hornbeam, birch and poplar in conditions of SAQP (chemical charge 7,0- 

7.5 %, T = 170°C, cooking time 60 min.) was performed in accordance with schedule 

of statistical planned experimentation Using direct determination of yield, lignin, 

2 

holocellulose and α-cellulose, the equations in the form y=b + b x + b x + b x + b x 0 1 1 2 2 3 3 11 1 

+ b 22 x 2 

2 + b33 x 3 

2 were obtained, where xi is content of wood species, their ratio and che- 

mical charge. Through mathematical processing of this formula we can determinate 

yield of SAQ semichemical pulp. 

Keywords: SAQP, yield, chemical composition

Posters 

.11

.12

4-P01 Efficient Solvent-Free Synthesis of 

Bis(arylmetylidene)piperidinones 


Posters 

M. Saeed Abaee, Mohammad M. Mojtahedi, Roholah Sharifi, 

A. Wahid Mesbah 

Chemistry and Chemical Engineering Research Center of Iran, 

P.O.Box 14335-186 Tehran Iran 

abaee@ccerci.ac.ir 

Crossed aldol condensation of homocyclic ketones with aldehydes is a useful 

method for the preparation of bis(arylmethylidene)cycloalkanones which are very 

important precursors in synthetic organic chemistry. In contrast, fewer investigations 

on similar heterocyclic counterparts have taken place. Despite the importance 

of these compounds from biological and bioorganic points of view, they have been 

prepared via the condensation of heterocyclic ketones with aromatic aldehydes 

under relatively harsh conditions and after long reaction time periods. 

In the present article, a 1:2 mixture of LiBr and diethylamine (Et 2 NH) is employed 

to induce one-pot double condensation of piperidinone with aromatic aldehydes 

under solvent-free conditions. Excellent yields of 3,5-bis(arylmethylidene) 

piperidinones are achieved in a facile one-pot general procedure. Structure of the 

products is determined by spectroscopic methods, elemental analysis, and X-ray 

crystallographic experiment. 

O 

N 

H 

ArCHO, r.t. 

LiBr, HNEt2 Keywords: bisarylmethylidene, piperidinone 

Ar 

O 

N 

H 

Ar 

.1


Posters 

4-P02 Novel Diketopyrrolopyrroles for Molecular 

Optical and Electrical Devices 

.1 

Pavel Bednář, Oldřich Zmeškal, Martin Weiter, Martin Vala 



bednar-p@fch.vutbr.cz 

Derivates of diketopyrrolopyrrole (DPPs) are relatively new materials exploited 

in the chemical industry as a high-performance pigments or dyes. They exhibit 

exceptional light, heat, chemical and weather fastness. Furthermore, some of their 

physical properties such as good film forming properties, absorption in visible area 

of light and high values of photoluminescence quantum yields are exceptional. 

The main goal of our work is a characterization of properties of the DPPs with 

respect to their potential application in the optoelectronical or electro-optical devices 

such as solar cells, photo-detectors, electroluminescent diodes and thin film 

transistors. 

The absorbance and photoluminescence measurements and current-voltage 

and electroluminescence measurements together with impedance spectroscopy 

were used to characterize these materials. 

The obtained results have shown that the DPPs are promising candidates for 

active electroluminescent materials or in solar cells. 

Keywords: diketopyrrolopyrrole, electroluminescence, photo-conductivity

4-P03 Diagnosis of Cirrhosis by GC/MS 

Monica Culea, Cornelia Mesaros, Eugen Culea 

“Babes-Bolyai” University, 

Str. M. Kogalniceanu, Nr. 1, Cluj-Napoca, 400084, Romania 



Posters 

Gas chromatographic-mass spectrometric (GC/MS) is one of the best method 

for measuring drugs. Applications are very important for purity control, pharmacokinetic 

studies, metabolic studies, clinical applications but also for treatment and 

diagnosis. Due to the high sensitivity needed, the selected ion monitoring (SIM) 

mode was used in caffeine test measurements. Caffeine and 15N-theophylline, the 

internal standard, were measured by using the isotopic dilution (ID) technique in 

5 minutes, on a Rtx-5 capillary column 30 m × 0.25 mm diameter, 0.25 μm filmthickness, 

in the temperature program: 200–250 °C, at 10 °C/min. A single dose of 

4 mg/kg p.o. of caffeine was followed by blood concentrations measurements at two 

points, 1 h and 9 h. Caffeine clearance, measured in patients with cirrhosis and chronic 

hepatitis, was reduced and half live time was increased in children with liver 

disease as compared with control. 

Keywords: cirrhosis, caffeine test, children, GC-MS 

.1


Posters 

4-P04 Leukotriene Quantitation by GC-MS 

.1 

Monica Culea, Eugen Culea, Paraschiva Chereches-Panta, Adela Pintea 

Babes-Bolyai University, 

Str. Kogalniceanu, nr.1, 3400 Cluj-Napoca 


Quantitation of eicosanoid levels in biological systems is important for understanding 

of their role in cell function or pathological and for diagnosis. Leukotrienes 

quantitation is limited by sensitivity of the method. Gas chromatography - mass 

spectrometry provides a sensitive and specific method when proper derivatization 

method is used. Gas chromatography was performed on a 5% phenyl methylpolysiloxane 

column (30 m × 0.25 mm I.D., 0.25 μm film thickness) operated in a 

suitable temperature program. Helium carrier gas was of 1ml/min. Ionization was 

performed by electron impact (EI) and detection in SIM mode. The method provided 

good response linearity and precision (< 30% C.V.) in the range 1–1000pg/ml, 

necessary to analyse leukotrene B4 in exhaled air. Arachidonic acid was used as 

internal standard.


Posters 

4-P05 Characterization of Oligosaccharides Using 

ESI-MS in Presence of Anions 

Richard Čmelík, Janette Bobáľová 

Institute of Analytical Chemistry, v.v.i., Academy of Sciences of the Czech Republic, 

Department of Proteomics and Glycomics, 

Veveří 97, CZ-60200 Brno, Czech Republic 

cmelik@iach.cz 

Knowledge of oligosaccharide sequence, inter-residual linkages, and branching 

are essential for understanding their biological functions and technological 

properties. 

We recently reported the study on comparison of the malto- and isomaltooligosaccharide 

mixtures using negative ion electrospray ionization (ESI − ) mass spectrometry 

(MS). Having the abundance of unstable [M–H] – ions that gives in-source 

fragment ions in ESI − mass spectra, oligosaccharides were stabilized by adduct formation 

[M+X] – with several anions (X – ). The analogous molecular mass distributions 

of presented glucose oligomers were obtained, whereas the chloride and nitrate adducts 

appeared in mass spectra in higher abundances relative to [M–H] – . 

Tandem MS experiments enable to distinguish α-(1→4) and α-(1→6) linkages 

on the base of specific diagnostic product ions. A fragmentation of [M+Cl] – and 

[M+NO 3 ] – ion adducts was found to be suitable for structural determination of (iso) 

maltooligosaccharides because more prominent cross-ring fragmentation was observed. 

Acknowledgement: This work was supported by the Project No. 2B06037 from the 

Ministry of Education, Youth and Sports, Czech Republic and Institutional Research 

Plan AV0Z40310501. 

Keywords: oligosaccharides, electrospray mass spectrometry, anionic adducts 

.1


Posters 

4-P06 A new Approach to Protein Enzymatic Digestion 

for Fast Protein Identification by Matrix- 

Assisted Laser Desorption/Ionization Time-of- 

Flight Mass Spectrometry 

.1 

Filip Dyčka, Markéta Laštovičková, and Janette Bobáľová 

Institute of Analytical Chemistry v.v.i., Academy of Sciences of the Czech Republic, 


dycka@iach.cz 

The combination of protein separation by polyacrylamide gel electrophoresis 

with mass spectrometric analysis of proteins digested enzymatically in-gel is a very 

powerful tool for protein identification in complex biological systems. Recently several 

approaches have been developed for fast protein digestion. One approach is the 

use of modified trypsin for in-gel digestion. Other promising approaches include 

microwave-assisted or ultrasonic assisted protein enzymatic digestion. The traditional 

sample preparation for protein identification through peptide formation is 

time-consuming procedure therefore we have studied and compared the variable 

performances of the fast enzymatic digestion of proteins using the different techniques. 

Most important, the new approach of protein digestion takes minutes, in 

contrast to several hours required by conventional methods. 

Acknowledgement: This work was supported by the grant from GAAV 

IAA600040701 and Research Plan of Institute of Analytical Chemistry, Academy of 

Sciences of the Czech Republic AV0Z40310501. 

Keywords: 1-D gel electrophoresis, proteins, in-gel digestion


Posters 

4-P07 The 13 C and 119 Sn NMR Spectra of Some 

Trioraganotin-(IV)-N,N-Diethyldithiocarbamates 

Used as Wood Preservatives 

Juraj Kizlink, Ladislav Reinprecht 


Purkyňova 118., CZ-612 00 Brno, 

kizlink@fch.vutbr.cz 

The trialkyl-, triaryl- and triaralkyl-tin-(IV)-N,N-diethyldithiocarbamates were 

synthetized and tested as wood preservatives against mould and rot - Coniophora 

puteana, Coriolus versicolor and Serpula lacrymans. The best results were obtained 

with the trialkyltin-(IV)-compounds in the order of R in the R 2 N- group: butyl-, 

propyl-, ethyl- and methyl- groups, comparable with commercial abroad widely 

used tributyltin-(IV)-naphtenate (TBTN). The 13 C and 119 Sn NMR spectra of these 

compounds were also measured and are here presented in the enclosed table. 

Keywords: triorgano-tin-N,N-diethyldithiocarbamates, wood preservatives, 

organotin biocides, 

.1


Posters 

4-P08 Preparation of Dimethyl Carbonate from 

Methanol and Carbon Dioxide – The Removal of 

Reaction Water by Olefins 

.20 

Juraj Kizlink 


Purkyňova 118., CZ-612 00 Brno 

e-mail: kizlink@fch.vutbr.cz 

For the utilization of carbon dioxide as significant waste, the dimethyl carbonate 

was prepared. Owing to the reaction water, this reaction is ceased in equilibrium. 

The removal of reaction water from the reaction mixture is possible either by chemical 

traps, or by means of some olefins, when the appropriate alcohols are produced 

during the reaction. The main product is useful as additive for lithium batteries, 

motor fuels and coolants for car air conditioners. It is also good methylation agent 

with very low mammalian toxicity. 

Keywords: dimethyl carbonate, dialkyl carbonates


Posters 

4-P09 Amadori Rearrangement for Synthesis of Chiral 

Fragments 

Jana Marková, Milan Potáček 

Masaryk University, Faculty of Science, Department of Chemistry 

Kotlářská 2, 611 37 Brno 

21510@mail.muni.cz 

Amadori rearrangement is reaction between sugars and amines. The rearrangement 

is based on the conversion of N-substituted glycosylamines to the Nsubstituted 

1-amino-1-deoxy-2-ketoses. Amadori discovered this reaction in years 

1926-31. The final structure of the rearranged product disclosed Weygand and Kuhn 

in 1937. Recently was found that Amadori rearrangement takes place in living cells 

and organisms, where sugars and amino acids coexist. There, Amadori rearrangement 

causes the functional modification of proteins, which are characteristic for 

diseases diabetes mellitus and arteriosclerosis. Amadori rearrangement is also known 

as initial phase of Maillard reaction and plays an important role in the synthesis of 

osazone, quinoxaline, folic acid and riboflavine. 

We have investigated conditions for Amadori rearrangement of D-glucose and 

5-deoxy-D-xylose in the presence of p-toluidine, benzylamine and dibenzylamine 

with different acid catalysts. We have found that p-toluidine and dibenzylamine 

only were able to enter into rearrangement. The products after rearrangement with 

p-toluidine possessed cyclic form. In the case of dibenzylamine the sugar was found 

in open form. For the removal of benzyl groups reduction by 10 % Pd/C was used. 

As products 1-amino-3,4,5,6-tetrahydroxyhexane-2-on and 1-amino-3,4-dihydroxypentane-2-on 

were prepared. These compounds can be used for the insertion of 

sugar stereogenic centers with hydroxyl groups to various organic molecules. In 

our case we intended to use this sugar chain to introduction to substituted pteridine 

moiety. 

Keywords: Amadori rearrangement, conditions, products 

.21


Posters 

4-P10 Solvent-Free Oxidative Deprotection 

of Trimethylsilyl Ethers with Calcium 

Hypochlorite 

.22 

Mohammad M. Mojtahedi, M. Saeed Abaee, Hassan Abbasi 



mojtahedi@ccerci.ac.ir 

Trimethylsilylation of alcohols is a very common practice in organic synthesis 

to protect hydroxyl groups. Such maneuver maintains the hydroxyl functionality 

and prevents its oxidation during reactions. However, sometimes, it is required to 

convert silyl ethers into their corresponding carbonyl structures. This conversion 

could be either carried out in a two step desilylation-oxidation process or directly 

achieved in a one pot reaction. 

In the present article, a considerably fast and mild microwave-assisted direct 

conversion of benzylic silyl ethers with calcium hypochlorite is reported under solvent-free 

conditions using montmorillonite K-10 solid support. 

Ca(ClO) 2 ,MW 

montmorillonite 

1a-i 2a-i 

Keywords: oxidative deprotection, calcium hypochlorite 

O


Posters 

4-P11 Ionic Liquid Promoted Synthesis of Benzofurans 

at Room-Temperature 

Ali Sharifi, M. Saeed Abaee, Bahram Zamiri, Mojtaba Mirzaei 



Sharifi@ccerci.ac.ir 

Benzofuran derivatives constitute highly valuable heterocyclic motifs found in 

the structure of many natural and synthetic products. Derivatives of these compounds 

are known to possess important pharmaceutical, antifungal, and antitumor 

properties. Additionally, benzofurans are used in cosmetic formulations and have 

application as precursors for optical brighteners. 

Although many methods are available for preparation of benzofurans, many 

of these methods are still conducted at high temperature, require the use of commercially 

unavailable starting materials, conducted in refluxing solvents, or need 

an external stimulant to proceed. In continuation of our previous experiences on 

environmentally sustainable reactions, we would like to herein report a novel procedure 

for ionic liquid promoted condensation of α-haloketones with various salicylaldehyde 

derivatives performed at room temperature in the presence of potassium 

carbonate. 

O 

O 

R O 

X 

R 

OH 

+ 

Y 

[hmim][PF 6] 

r.t.; K 

Cl 2CO3 X 

O 

R = H, Me 

X = H, OMe, NO2, Br 

Y = H, Cl, Br, OMe 50-98% 

Keywords: benzofurans, ionic liquid 

Y 

.2


Posters 

4-P12 Crystal Engineering of Glycoluril Dimers 

.2 

Marek Štancl, Vladimír Šindelář 

Department of Chemistry, Masaryk University 

Kotlářská 2, 611 37 Brno, Czech Republic 


Glycolurils are bicyclic heterocycles, which have found application as building 

blocks of many supramolecular objects e.g., macrocyclic cucurbiturils (CB). Fundamental 

building block of the CB is methylene-bridged glycoluril dimer. So far, only 

the glycoluril dimers bearing various groups in the position 7 and 8 appeared in 

literature. Herein we report the synthesis of methylene-bridged glycoluril dimers, 

which better resemble CB. Selective method for the synthesis of 1,6-substituted glycolurils 

has been developed. The glycolurils have been used for the synthesis of 

methylelne-bridged glycoluril dimers bearing hydrogen atoms on their convex face. 

Depending on the side walls of the dimer different self-assembly in the solid state 

have been described using X-ray crystallography. 

Keywords: glycoluril, glycoluril dimer

Section 5: 

Photochemistry 

.1

Lectures 

.

Section – Photochemistry 

Lectures 

5-L01 EPR and UV Study on Photocatalytic Activity of 

Titanium Dioxide Powders 

Vlasta Brezová, Zuzana Vrecková, Dana Dvoranová 

Institute of Physical Chemistry and Chemical Physics, Faculty of Chemical and Food Technology, 



vlasta.brezova@stuba.sk 

The detoxification of polluted water by semiconductor photocatalysis based on 

the photoexcitation of titanium dioxide is nowadays accepted as promising technique 

also for industrial applications. The degradation of a variety of toxic organic 

contaminants is caused by the non-selective attack of hydroxyl radicals produced 

upon ultra-band gap irradiation of TiO 2 . 

The formation of photoinduced free radicals was monitored by in situ EPR spectroscopy, 

using as indicators N-oxide and nitrone spin trapping agents. EPR spin trapping 

technique confirmed the photoinduced formation of spin adducts, which Hamiltonian 

parameters are in agreement with addition of hydroxyl radical to the double bond 

of spin traps. The ability to generate free radicals upon TiO 2 photoexcitation was also 

followed by semi-stable free radicals (Tempol, ABTS •+ , DPPH), and the TiO 2 photocatalytic 

activity was indicated here by a continuous decrease of radical concentrations. 

The effective generation of hydroxyl radicals on TiO 2 surface primary requires adequate 

absorption of incident radiation by TiO 2 , which is strongly influenced by the optical 

properties of TiO 2 aqueous suspensions. Consequently, the absorption and scattering 

coefficients of TiO 2 samples were evaluated from the measured extinction spectra using 

Kubelka-Munk analysis. The data obtained by EPR and UV spectroscopy served as a 

basis for the calculation of quantum efficiency of photocatalytic reactions on TiO 2 . 

Keywords: TiO 2 , EPR spectroscopy, UV spectroscopy 

.


Lectures 

5-L02 Photocatalytic Degradation of Formic Acid on 

TiO 2 thin Layers 

. 

Jana Chomoucká, Petr Dzik, Michal Veselý, Eric Puzenat and Chantal 

Guillard 

Faculty of chemistry, Institute of Physical and Applied Chemistry, 


chomoucka@fch.vutbr.cz 

In recent years, many studies have been focused on the photocatalytic degradation 

of organic compounds mediated by semiconductor particles acting as photocatalysts. 

In particular, titanium dioxide has been widely employed, thanks to its 

outstanding photocatalytic activity and hight stability. 

TiO 2 thin films were deposited onto borosilicate glass plates by sol-gel technique 

using dip-coating and printing methods. Formic acid (FA) was choosen because 

of simple mechanism of degradation: it undergoes direct mineralisation to 

CO 2 and H 2 O without the formation of any stable intermediate species. Moreover, 

it also represents a possible final step in the photodegradation of more complex 

organic compounds. Photocatalytic activity was evaluated by examining the oxidation 

rate of formic acid aqueous solution upon UV irradiation in a pyrex cylindric 

reactor. HPLC analysis was used for detection of FA concentration. The obtained 

values of initial reaction rate for various FA initial concentrations were treated using 

Lagmuir-Hinshelwood model. The influence of TiO 2 layer number (dip-coating 

method) and the value of TiO 2 dot area (printing method) on the rate of FA photocatalytic 

degradation were tested. 

The photocatalytic performances of TiO 2 coatings were correlated to their 

physico-chemical properties. 

Keywords: photoctalytic degradation, formic acid


Lectures 

5-L03 Kinetic of Degradation of Historical Documents 

Containing Iron-Gall Inks 

Michal Čeppan, Viera Jančovičová, Milena Reháková, Andrej Buzinkay 



michal.ceppan@stuba.sk 

Deterioration of paper documents containing iron-gall inks is supposed to be a 

combination of two degradation pathways – acid hydrolysis of cellulose and oxidative 

degradation of cellulose. Iron gall inks contain transition metals, such as iron 

and copper, catalyzing the radical oxidation of the substrate, as well as acids, catalyzing 

its hydrolysis. Transition metals are capable to enter Fenton and Fenton-like 

type of reactions and catalyze homolytic decomposition of peroxides with production 

of hydroxyl radicals. Hydroxyl radicals can oxidize cellulose and induce degradation 

of cellulose. 

The influence of copper ions in iron-gall inks on the rate of degradation and 

change of optical properties of aged model documents were studied. Rate constants 

of degradation of systems containing various ratio Cu:Fe in a range of pH 3-6 were 

determined using viscometric measurements and Ekenstam equation. It was found, 

that in pH around 3 the ratio Cu:Fe in inks does not significantly affect the rate of 

degradation and probably acid hydrolysis is predominant pathway of degradation. 

At higher pH the rate of degradation depends on the ratio Cu:Fe in inks and oxidation 

degradation is more significant. The change of colorimetric properties (CIE Lab 

lightness, chroma and hue angle) during aging also point out to the role of oxidative 

degradation in the range of higher pH. 

Keywords: iron gall inks, degradation of paper 

.


Lectures 

5-L04 Photochemical Transformations of Anticancer 

Drug Irinotecan 

. 

Dana Dvoranová, Vlasta Brezová, 

Zuzana Vrecková, Marián Valko 




dana.dvoranova@stuba.sk 

Irinotecan (CPT-11 or Camptosar ® ) used in the clinical cancer treatment, is a 

water-soluble semisynthetic analogue of natural quinoline alkaloid camptothecin. 

Molecule of CPT-11 represents a pro-drug converted in vivo to its active metabolite 

acting as an antineoplastic agent inhibiting the eukaryotic DNA topoisomerase I. 

Previously, it has been proposed that coincident presence of transition metal ions 

and UVA radiation enhanced the cytotoxic activity of several anticancer drugs possessing 

quinoline-like structure. 

Our investigation was focused on photochemical transformations of CPT-11 

in aqueous and dimethylsulfoxide solutions, as well as on the influence of different 

concentrations of Cu(II) ions added into photochemical system. The deoxygenated 

and aerated solutions of CPT-11 and Cu(II)/CPT-11 were UVA photoexcited 

(λ > 300 nm), and the photoinduced changes were monitored by means of EPR and 

UV/Vis spectroscopy. EPR spin trapping technique with different types of spin trap 

agents serves as a tool to identify photoproduced reactive radicals. 

Keywords: Irinotecan, EPR spectroscopy, Cu(II) ions

5-L05 Thin Layers of TiO 2 Prepared 

by Inkjet Printing 

Petr Dzik, Jana Chomoucká, Michal Veselý 



petr@dzik.cz 


Lectures 

Transparent thin layers of photocatalytic TiO 2 were prepared using conventional 

sol-gel chemistry and novel method of sol delivery to the substrate. We used 

a well known sol chemistry based on tetraisopropoxy titanate and acetylacetone as 

stabilizer. Prepared sol was loaded into an ordinary office inkjet printer equipped 

with piezoelectric print head. Sol was then printed onto pyrex glass plates, gelled at 

110 °C and calcinated at 450 °C. Thus we prepared transparent thin layers of TiO 2 of 

varying thickness and surface morphology. 

Properties of prepared layers were studied using optical microscopy, SEM and 

AFM. Photocatalytic performance was evaluated by the rate of DCIP decomposition 

and surface properties were studied by contact angle change. 

In this way we were able to prepare thin layers of TiO 2 with excellent optical 

properties and photocatalytic performance comparable to dip- or spin-coated 

layers. Inkjet printing proved to be very elegant and clean method for sol deposition 

with minimum waste. Unlike the traditional methods of dip- and spin-coating, inkjet 

printing is capable of coating large areas of substrate, being limited only by the 

width of used inkjet printer. 

Keywords: TiO 2 , photocatalysis, sol-gel, inkjet printer, 

.


Lectures 

5-L06 Photocatalytic Properties of TiO 2 and 

Photosensitizing Materials for Bactericidal 

Applications 

.10 

C. Guillard, K. Benabbou, T.-H. Bui, T. Pigot, S. Pigeot-Remy, P. Lejeune, 

S. Lacombe 

Institut de recherche sur la catalyse et l’environnement de Lyon (IRCELYON) 

Université Lyon 1, UMR 5256 CNRS 

2 Avenue Albert Einstein 2 

Villeurbanne cedex France. 

chantal.guillard@ircelyon.univ-lyon1.fr 

Several water disinfection technologies are available, such as chlorination and 

ozonation However, in addition to their high costs, they can lead to the formation of 

harmful disinfection by-products (DBPs), among the most dangerous of which are 

the trihalomethanes (THMs), well-known for their high carcinogenic potential [1]. 

Among the new oxidation methods or “Advanced Oxidation Processes” (AOP), 

heterogeneous photocatalysis appears as an emerging destructive technology leading 

to the total mineralization of pollutants at ambient temperature and atmospheric 

pressure using a cheap photocatalyst, TiO , having the possibility of using a part 

2 

of solar energy as the UV source. Its principle is based on the formation of highly 

reactive oxygen species (ROS), such as OH • , O 2 

•– HO2 

• , capable of destroying chemi- 

cal and biological water contaminants, under irradiation of semiconductor particles 

(photocatalyst) with photons of energy higher than or equal to its band gap energy. 

Other types of catalysts begin to appear, such as photosensitizing materials 

which generated singlet oxygen under UV but also visible light. 

In this work, we studied the inactivation of E. coli using industrial TiO 2 photocatalysts, 

Degussa P25 and Millennium PC500, either suspended or supported and 

different sensitizing materials prepared by using sensitizers such as anthraquinone, 

9,9-dicyanoanthracene, 4-benzoylbenzoic acid encapsulated in silica matrices. The 

influence of parameters, such as the nature and amount of the sensitizer or TiO 2 and 

of the irradiation intensity was investigated. 

Keywords: photocatalysis, TiO 2 , sensitizing materials, desinfection, E. coli

5-L07 Promising Perspectives in Photocatalysis 

Chantal Guillard, Eric Puzenat, Jean-Marie Herrmann 

IRCELYON, UMR CNRS 5634, 

Université de Lyon 1, 2 av. Albert Einstein, Villeurbanne cedex, 69626, France, 

Chantal.guillard@ircelyon.univ-lyon1.fr 


Lectures 

Heterogeneous Photocatalysis based on titania originated some 40 years ago 

but the interest of industry for environmental applications emerged only 10 years 

ago. These applications mainly concerned self-cleaning materials and water and air 

purification. However, norms are required. For instance, French AFNOR created the 

B44-A photocatalytic commission in September 2007, which is composed of scientists 

from National Laboratories including one of us (C. G.) and from industry. In 

the first part of the talk, the active species generated at the surface of a photocatalyst 

will be presented under different reaction conditions (liquid phase (water, solvents) 

and gas phase), corresponding to dedicated applications such as air and water treatments, 

self-cleaning materials, fine chemistry, hydrogen production, etc... 

The comparison of reaction mechanisms occurring in equeous and gas phases 

is made possible by identifying the intermediate products formed during the degradation 

of pollutants in both phases. The prediction of reaction pathways in aqueous 

phase is based on the attack by OH° radicals on different families of organic pollutants 

and subsequently verified by the experiments. Some applications of photocatalysis 

(decoloration of dyes, detoxification of water, use of solar energy, air treatment 

(VOC and odor removal), self-cleaning materials), developed at IRCELYON 

will be presented and put in realistic perspectives. 

Keywords: photocatalysis, water and air treatment, selfcleaning materials, 

disinfection 

.11


Lectures 

5-L08 Interactions in Iron Gall Inks in Air and 

Nitrogen Atmosphere 

.12 

Viera Jančovičová, Michaela Ciglanská, Bohuslava Havlínová, 

Michal Čeppan 

Department of Printing Arts Technology and Photochemistry IPM, 

Faculty of Chemical and Food Technology SUT, 


viera.jancovicova@stuba.sk 

The iron gall inks were one of the most important writing means used from the 

ancient times through the Middle Ages until the modern times. For preparation of 

iron gall inks, gall nuts (polyphenols), vitriol (ferrous sulphate), gum Arabic, water, 

sometimes wine, beer, or vinegar as solvents, were used. 

Simple models of iron gall inks composed of gallic acid, ferrous sulphate, and 

gum Arabic in various relations were prepared. The process of iron ion complex 

formation with gallic acid on air and under nitrogen atmosphere was investigated 

using UV Vis spectroscopy, pH measurements, and by monitoring the concentration 

changes of Fe(II) ions. The admixture of Fe(II) ions to gallic acid on air induced a 

bathochromic shift of absorbance bands in UV region and formation of new absorbance 

band in the visible region following the colour complex formation. Nitrogen 

atmosphere significantly retarded this reaction and the samples remained colorless. 

Keywords: iron gall inks, complex formation, spectral properties


Lectures 

5-L09 Reactivity of Hydroxyl Radical on Benzene 

Ring and Side Chain in the Photocatalytic 

Degradation of Phenylurea Derivatives 

Jaromír Jirkovský, Gottfried Grabner 

J. Heyrovský Institute of Physical Chemistry, Dolejškova 3, 

CZ-182 23 Prague, Czech Republic 

jaromir.jirkovsky@jh-inst.cas.cz 

Mechanistic studies of the photocatalytic degradation of some ring-halogenated 

and side-chain-substituted phenylurea derivatives on nanocrystalline titanium 

dioxide have been carried out. Several products resulting from oxidation of the aliphatic 

side chain induced by hydroxyl radical were identified using HPLC. A detailed 

picture of side-chain reactions proceeding via two successive demethylation 

steps could be established. Degradation proceeded according to first-order kinetics, 

which means that the system remained in a photostationary state with respect to 

hydroxyl radical concentration during the whole reaction sequence. Quantitative 

analysis revealed that the side-chain reactions represent only 30 % of the total photodegradation, 

the remainder being due to reaction on the benzene ring leading to 

ring-opening products, which escaped HPLC detection. In order to get more information 

on the relative amount of ring vs. side chain attack, laser flash photolysis was 

used. Hydroxyl radicals were generated by photoionization of [Fe(CN) 6 ] 4- in neutral 

aqueous solution saturated with N 2 O; they then reacted either with [Fe(CN) 6 ] 4- to 

give [Fe(CN) 6 ] 3- or with the phenylurea derivative on the ring or at the side chain. 

The first two of these reactions could be followed by formation of the products 

([Fe(CN) 6 ] 3- and OH adducts). 

Experimental results obtained by varying reactant concentrations were fitted 

using nonlinear regression. As a result, the rate constant for reaction of hydroxyl 

radical on the ring was found to decrease with the number of Cl substituents 

while that for reaction at the side chain increased with the number of abstractable 

H atoms. 

.1


Lectures 

5-L10 Approaches to standard photocatalyst tests in 

gaseous and liquid phase 

.1 

Josef Krýsa 

Institute of Chemical Technology, Department of Inorganic Technology, 

Technická 5, Prague 6, CZ-166 28, 

Josef.Krysa@vscht.cz 

Photoactivity of TiO 2 can be understood as the ability to form superhydrophilic 

surface (self-cleaning properties), the ability to inactivate microorganisms (antibacterial 

properties) and the ability to oxidise (degrade) organic pollutants. These pollutants may 

be present in various phases. In air (malodorous substances, volatile solvents), in waters 

(dyes, aromatics, chlorinated hydrocarbons, pesticides) and in the form of compact films 

(methyl stearate, oleic acid). 

This paper presents some approaches to standard photocatalyst tests in gaseous 

and liquid phase. The choice of suitable model compounds as acetone, hexan, 

toluene for gas phase and 4-chlorophenol, dyes Acid Orange 7, Methylene Blue, 

herbicides monuron, atrazine for liquid phase is critically discussed together with 

the selection of UV light source and photoreactor design. The demand for analytical 

instrumentation together with the method of photoactivity expression is also 

assessed. 

Keywords: photocatalysis, gaseous, liquid phase


Lectures 

5L-11 Testing network for verification of photocatalytic 

products and technologies for 

environmental benefit – PETV 

František Peterka 

NANOPIN-ATG (National Research Centre For Nanosurfaces Engineering), 

Nad Kazankou 11 ,171 00 Praha 7-Troja ,Czech Republic 

fpet@mbox.vol.cz 

The purpose of technology verification is to check technology performance 

data by an authorized third party using specified technology verification protocols. 

A successful verification should deliver an independent proof that the technology 

performance claim of the technology provider is correct. Efforts in this field are 

presently being made by some national and international technical committees. 

Standardisation must answer two questions: the first is market-oriented because 

it has to take care of the final user expectation and to reflect producer/seller 

advertising/market penetration requirements; the second is to answer on the suitability 

of a product for use requirements (efficacy, chemical durability, mechanical 

properties) and on the assessment for the type of application (materials, goods, 

substrates). Common to all standardization tests is the principle that standard tests 

have to avoid discrimination, which is a preferential evaluation for some materials 

depending on the test conditions. 

Standardisation action as proposed in this project (PETV) will serve to the enhanced 

competitiveness of European industry in order to take a leading role in this 

domain of photo-catalysis technology and its industrial application. 

Keywords: photocatalysis, application, verification, standardization 

.1


Lectures 

5-L12 Evaluation of the Photocatalytic Properties 

of TiO 2 Thin Film Layers. Application to Selfcleaning 

Glass 

.1 

E. Puzenat, J. Drbohlavová, L. Péruchon, J.-M. Herrmann and C. Guillard 

IRCELYON UMR CNRS 5256, 

Université de Lyon, 2 av. Albert Einstein, Villeurbanne cedex, 69626, France, 

eric.puzenat@ircelyon.univ-lyon1.fr 

During the last fifteen years, photocatalytic applications have been mainly developed 

in the fields of water and air treatments. Beside these two fields, self-cleaning 

materials have been extensively studied and are now commercialized. Nevertheless, 

the complete understanding of the mechanisms occurring at their surface is 

still missing. The present communication is focused on self-cleaning glasses. These 

glasses are based on the photocatalytic and superhydrophilic properties of a submicronic 

layer of photocatalytic TiO deposited on their surface. Under natural or ar- 

2 

tificial UV-irradiation, electrons and holes photogenerated insideTiO produce free 

2 

radicals (OH• •– , O ...) in the double presence of oxygen and water. These radicals are 

2 

able to induce oxidation of the organic stains adsorbed on the glass surface, thus 

reducing their adherence. Moreover, under UV-irradiation, TiO layers acquired su- 

2 

perhydrophilic properties leading to the spreading out of the water droplets at the 

surface and consequently to the formation of a water thin film. 

Tests in liquid, gaseous and deposited phases have been performed to evaluate 

the photocatalytic properties of TiO thin film layers. Intrinsic characteristics of the 

2 

layers such as preparation method, thickness or porosity have been related to the 

efficiency of the layers. Moreover, environmental parameters such as the temperature, 

the wavelength range, the light intensity, the presence of mineral salts and the 

nature of organic pollutant families (fatty acids, heavy alkanes and various PAH’s 

(Polycyclic Aromatic Hydrocarbons)) has been studied to first better understand the 

photocatalytic processes occurring at the surface of the glass and secondly to help 

the establishment of criteria for a normalized test.


Lectures 

5-L13 Study of Stabilization of Documents Containing 

Iron Gall Inks by Treatment of Atmospheric DBD 

N 2 Plasma 

Milena Reháková, Michal Čeppan, Milan Mikula 

Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology 


milema.rehakova@stuba.sk 

The aim of this experimental work was to investigate the possibilities of plasmo-chemical 

methods use on stabilization documents containing iron gall inks. The 

work was concentrated on enhancement of strength and stability of historical paper 

documents treated in dielectric barrier discharge (DBD) at atmospheric pressure in 

combination with natural polymer chitosan and/or anti-oxidizing agent. 

The mechanical properties - holding endurance, breaking length, chemical 

properties - pH of aqueous extracts, degree of polymerization and optical properties 

- the coordinates of colour space, total colour difference (fading) of modified 

papers with iron gall inks were tested. The method of accelerated ageing in airtight 

glass tubes at an elevated temperature for a certain time was used. Influence of 

components of modification systems (neutralizing and strengthening substances, 

antioxidant) was considered. 

Keywords: iron gall inks, ageing 

.1


Lectures 

5-L14 An Overview of Inkjet Printouts Accelerated 

Ageing Methods 

.1 



Purkyňova 118, Brno 61200 

xcstancik@fch.vutbr.cz 

Accelerated ageing is a commonly used method for predicting the lifetime of 

various materials from many branches of industry. It is a necessary element of material 

testing in the textile or automotive industry for years. Changes came with the 

progress of printed photos, new standards and procedures had to be found for testing 

printouts. 

Standards for classic photography or offset print cannot be applied for inkjet 

print because of different behavior of ink used in inkjet printers. The adjustments of 

used standards are in progress recently. 

This paper brings an overview of the various factors that affect the permanence 

of digitally printed printouts. Various types of accelerated tests methods are discussed 

with respect to past and current standards. Some problems of printouts accelerated 

ageing are discussed too. 

Keywords: accelerated ageing tests, inkjet print.


Lectures 

5-L15 Subjective and Objective Evaluation of 

Recognisability of Black Character on Paper 

A. Szitás, M. Jablonský, S. Katuščák S., 


Slovak University of Technology in Bratislava, Radlinskeho 9, 

SK-812 37 Bratislava, Slovak Republic 

attila.szitas@stuba.sk 

Generally it is believed that the recognisability of black character on paper humano 

modo (RS) dependens on the contrast between text character and background 

(∆EC-B). Antropomorphous colour systems such as CIE Lab has been developed to 

express the total and partial difference in human-like manner. The contrast can be 

express by total colour difference (∆E), or difference of brightness (∆B), lightness 

(∆L*). By psychometric measurement it was observed that dependence between 

∆EC-B and subjective abilitz of observer to see and recognize charakter is non linear 

and in the zone of košer values of kontrast is not valid at all. RS doesn´t drop with 

decrease of the contrast, or can even rise. In the specific zone of optical contrasts 

doesn´t apply recognisability of black character on paper goes down with decrease 

of ∆EC-B. This paper was to verify dependence between objective ∆EC-B and visual 

subjective RS with constants blackness on paper background with variable brightness 

(B), lightness (L*) or blackness (Bb, MSWord) by measurement of correctly 

recognised characters focus the due observer distance (l). The observed dependence 

of recognisability black character on the color difference ∆EC-B is described as 

RS = 503,45.(0,3363. (∆EC-B)2 – 37,246∆EC-B + 1514,069)-1 

Keywords: recognisability, contrast 

.1


Lectures 

5-L16 Pigments for Photocatalytic Paints 

.20 

Jan Šubrt 

Ústav anorganické chemie AV ČR 

250 68 Řež, Czech Republic 

subrt@iic.cas.cz 

During recent years environmental problems play an important role in the applications 

of titania pigments. These include the use of their photocatalytic behavior 

in the development of self-cleaning surfaces for buildings, i.e. antisoiling and antifungal 

growth and NO X emissions reduction. In terms of self-cleaning paints, the 

idea is to limit the oxidation and chalking of the paint film to the very near surface 

layers such that over time with weathering rain water will wash the top layer leaving 

an underlying clean fresh surface. In this regard, mixtures of pigmentary rutile 

and nanoparticle anatase pigments appear to provide the best option, with the former 

inducing some level of base stability, whereas the presence of the latter gives 

rise to surface activity. 

We tested various types of nanocrystalline anatase with high photoactivity 

in a silicate based photocatalytic paint system in order to elucidate the properties 

necessary to achieve high level of self-cleaning activity. After three years of testing 

under natural conditions the paints show different behavior in dependence on the 

nature of the photoactive nanocrystalline titania used. The self-cleaning activity of 

the paints depends not only on the photoactivity of the pigment, but mainly on the 

strength of binding among the nanoparticles in the photoactive anatase pigment. 

Keywords: photocatalytic paints, anatase

5-L17 Organometallic Cationic Photoinitiators 


Lectures 

Jan Vališ, Bohumil Jašúrek, Tomáš Syrový, Katarína Remenárová, 

Tomáš Weidlich 

University of Pardubice, Faculty of Chemical Technology, 

Department of Graphic Arts and Photophysics, 

Studentská 95, 532 10 Pardubice, Czech Republic 

jan.valis@upce.cz 

Several arene-iron complexes ((η 5 -2,4-cyclopentadien-1-yl)[(1,2,3,4,5,6-η)arene] 

ferrous hexafluorophosphate) were tested as photoinitiators for cationic polymerization. 

The UV light decomposes arene-ironcyclopentadienyl (arene-Fe + Cp) 

cation selectively to the corresponding aromatic compound and iron cyclopentadiene 

(CpFe + ) cation. Structure of aromatic compound affects decomposition upon UV 

light. FeCp + formed during irradiation is active species for cationic polymerization. 

Decomposition of arene-iron initiators upon UV light was followed by UV-VIS 

spectroscopy. It was measured the decrease of absorption band at about 245 nm 

after irradiation 2∙10 -4 M solution in acetonitrile. Super pressure mercury lamp was 

used as source of UV radiation. Extinction coefficients and energies need for achieving 

50 % conversion were calculated for all initiators. 

Efficiency of polymerization initiated by tested photoinitiators was followed by 

FTIR spectroscopy in vinylether and epoxide monomers. Results from FTIR showed 

that most of prepared organometallic photoinitiators can be used for initiation cationic 

UV polymerization with similar efficiency as commercial initiator Irgacure 

261. 

Keywords: cationic photoinitiator, UV polymerization, UV-VIS spectroscopy 

.21


Lectures 

5-L18 Kinetics of Oxidative Processes on Inkjet- 

Printed thin Layers of Titanium Dioxide 

.22 

Michal Veselý, Petr Dzik, Mária Veselá, 

Jana Drbohlavová, Jana Chomoucká 



vesely-m@fch.vutbr.cz 

Photocatalytic reactions with TiO 2 are used for organic compounds oxidation, 

metal reduction, disinfection of various surfaces and water. As the photogenerated 

active oxygen species are formed at the irradiated TiO 2 surface, this system can be 

utilized for microbes deactivation instead of the conventional methods such as UV 

irradiation, heat treatment or chemical disinfectant application. 

We used photocatalytic flow reactors for organic compound degradation and 

water sterilization. The photocatalytic flow reactor was designed as a system of parallel 

glass tubes, connected together by knee fittings permitting the studied suspension 

to circulate through the whole system. A successful photocatalytic reaction 

requires approaching of microorganism cells to the irradiated surface at a very close 

distance. Only under these conditions the photogenerated species can react with the 

cells. Therefore, optimal flow parameters, determining an effective adsorption on 

the irradiated surface were studied. Under these conditions the photocatalytic process 

of yeast killing was performed. 

Disinfection of solid surfaces is also of great interest. We performed experiments 

of yeast photokilling on printed titanium dioxide surface. The kinetics of both 

photocatalytic disinfection processes was studied and evaluated in dependence of 

surface structure of printed layers. 

Keywords: titanium dioxide, printed layer, yeasts, kinetics


Lectures 

5-L19 Model dyes in Titania photocatalytic systems 

Jiří Zita and Josef Krýsa 

Institute of Chemical Technology, Department of Inorganic Technology, Technická 5, Prague 6, 

CZ-166 28, Jiri.Zita@vscht.cz 

In order to compare the effectiveness of one photocatalytic layer with that of 

another, and to be able to effect and demonstrate a commercially acceptable high 

degree of quality control on any SPC (semiconductor photocatalysis) product, such 

as self-cleaning glass, it is essential to have a set of agreed standard methods of 

assessing SPC activity. The creation, validation and promotion of such a set of standard 

methods is currently in progress by an internationally-recognised body (ISO; 

TC206). 

Although the methods under consideration are not yet in the public domain, it 

appears timely to consider briefly some of those which have proved most popular 

in the past and one that has just recently been reported and appears most easily effected 

and to have a real future 

As a consequence Orange II, Methylene Blue, Resazurin and Plasmocorinth B 

appears ideally suited as a test reagents for assessing the photocatalytic activity of 

titania films, such as generated in this work. 

Keywords: photocatalysis, model dyes 

.2


Lectures 

5-L20 A Study on the Thickness Homogeneity and 

Refractive Index of Thin Organic Layers 

.2 

Oldřich Zmeškal, Ota Salyk, Michal Veselý, Petr Dzik 


Faculty of Chemistry, Purkynova 118, 612 00 Brno 

zmeskal@fch.vutbr.cz 

This paper deals with the utilization of optical and interference microscopy for 

the study of thin layers. We present a new and very simple method for the determination 

of refractive indexes of transparent layers based on interference phenomena. 

Both thin layer thickness and refractive index can be determined by this method. 

We used this method for a complex analysis of sandwich and gap structures of 

organic semiconductor components (e.g. ITO/PEDOT/DPP/Alq 3 /Al) prepared by a 

combination of following procedures: vacuum vapour deposition and/or spin coating 

and/or inkjet printing. A series of photographs of one sample was recorded and 

analysed which gave us the thicknesses of individual layers, their homogeneity and 

their refractive indexes. 

Recorded images were processed by means of image analysis (correlative and 

fractal analysis). Image analysis was performed using application HarFA (Harmonic 

and Fractal Image Analyser), which has a wide potential of use not only for image 

analysis but for analysis of video clips, signals and numerical data as well. 

Results obtained by image analysis methods were confirmed and eventually 

further extended by ellipsometric measurements, which is unfortunately more time 

consuming and challenging. 

Keywords: organic thin films, refractive index, layer thickness, image, fractal, 

correlation analysis, ellipsometry

Posters 

.2


Posters 

5-P01 Using of Vis-Nir Fibre Optics Reflectance 

Spectra for Identification of Iron-Gall Inks in 

Historical Documents 

Michal Čeppan, Daniela Pavlisová, Milena Reháková, Jarmila Dóriová 



michal.ceppan@stuba.sk 

Absorption molecular spectroscopy provides useful tools for analysis of historical 

documents. Among others VIS-NIR reflectance spectroscopy is used to identify 

the presence of particular inks. Measured spectra sets are generally complex in their 

nature and the extraction of useful chemical information is not straightforward. The 

resolution of spectra sets and extraction of useful information can be enhanced by 

chemometric processing using multivariate factor analysis methods 

In this paper previously developed method of factor analysis was used to process 

VIS-NIR fibre optics reflectance spectra measured on historical drawings and 

documents with grey-brown inks and identification of iron gall inks. 

Iron gall inks containing transition metals, such as iron and copper, are catalyzers 

of degradation processes, generally known as “ink corrosion”, so their identification 

in drawings is of importance to understand the history and aesthetics of an 

object, to evaluate the risks involved with its use, and to judge the effects of conservation 

treatments. 

The results of spectroscopic investigation were validate by XRF spectroscopy 

and chemical spot tests. 

Keywords: factor analysis, historical documents, VIS-NIR fibre optics reflectance 

spectroscopy 

.2


Posters 

5-P02 The Study of TiO 2 thin Films Photocatalytic 

Activity on Degradation of Yeast and Dye 

Pollutants 

.2 

Jana Chomoucká, Jana Drbohlavová, Petr Dzik, Mária Veselá, 

Michal Veselý 

Faculty of chemistry, Institute of Physical and Applied Chemistry 


chomoucka@fch.vutbr.cz 

Among various oxide semiconductor photocatalysts, titanium dioxide (TiO ) 2 

appears to be a promising and important prospect for use in enviromental purification, 

because of its strong oxidizing power, photoinduced hydrophilicity, nontoxicity 

and long-term photostability. 

The antimicrobial properties of TiO thin films were tested on photocatalytic 

2 

killing of yeasts Candida tropicalis and Candida albicans. Generally, Candida is the most 

common cause of opportunistic mycoses (Candidiasis) worldwide. The antimicrobial 

effect of photocatalysis with TiO could be due to the presence of reactive oxygen 

2 

•– species (ROS), such as superoxide (O ), hydrogen peroxide (H2O ), and hydroxyl 

2 

2 

radical (HO• ), generated either by illuminated TiO or by the illumination (mainly 

2 

UV) of the cells. TiO layers were immobilised by dip-coating method on soda- 

2 

lime glass plates using sol-gel method with titanium tetraisopropoxide precursor 

in ethanol. The dependence of Candida tropicalis and Candida albicans survival ratio 

on irradiation time was determined by epifluorescent microscopy using Acridine 

Orange dye. 

The influence of TiO layer number on the photocatalytic oxidation 2,6-di- 

2 

chloindophenol was also studied in a desk reactor. The concentration decrease of 

2,6-DCIP was evaluated spectrofotometrically. 

Keywords: titanium dioxide, photocatalytic killing


Posters 

5-P03 The Influence of Photoinitiators and Aditives 

on the Photochemical Speed of Methacrylated 

PVAl 

Marcela Černá, Petr Dzik, Michal Veselý 



xccernam@fch.vutbr.cz 

This study deals with the cross-linking reaction of methacrylated poly(vinyl 

alcohol) (PVAl) by UV initiated non-linear polymerization in solid state. PVAl 

was modified by the glycidyl methacrylate. During this reaction the copolymer 

poly(vinyl alcohol-co-vinyl glycidyl methacrylate) was created. 

A thin layer of methacrylated PVAl solution was spin-coated onto anodized 

aluminum plates. Plates were exposed by UV radiation and cross-polymerization 

took place. We compared the effect of three different commercial photoinitiators. 

The concentration of each photoinitiator was varied from 0.5 % to 4 % (w/w) and the 

influence of the photoinitiator amount on photochemical speed of modified PVAl 

was investigated. 

Apart from the photoinitiator influence, we also investigated thermal properties 

of studied systems. It was found that crosslinking polymerization took place 

well below Tg. In order to perform the crosslinking reaction above Tg, we added 

different types and concentrations of plasticizers. The plasticizers caused a shift in 

the glass transition temperature. The shift was determined by the DSC (differential 

scanning calorimetry) method. The optimum dose of used aditives has been evaluated. 

.2


Posters 

5-P04 TiO 2 Based Sensor with Nanostructured Surface 

for Gas Detection 

. 0 

Jana Drbohlavová, Jaromír Hubálek, Marina Vorozhtsova, Radim Hrdý 

Brno University of Technology, Faculty of Electrical Engineering and Communication, 

Departement of Microelectronics 

Údolní 53, 602 00 Brno, Czech Republic 

drbohla@feec.vutbr.cz 

Generally, solid semiconductor metal oxides (SMO) are used as a convenient 

material for monitoring the quality of air by means of different gases detection (O 2 , 

H 2 , CO, NH 3 , NO x or hydrocarbons). We designed a new high sensitive gas sensor 

with titanium dioxide coating on nanostructure-modified surface of gold electrode. 

Nanorod-structured surface of Au electrode was fabricated using Al 2 O 3 nanoporous 

template sputtered on thick Au substrate. Before TiO 2 deposition, prepared 

nanorod-stucture modified electrode was fixed on corundum plate with thick-film 

platinum meander heater using glass paste. Finally, TiO 2 coatings were immobilized 

employing a sol-gel technology starting from titanium tetrapropoxide precursor, 

ethanol as solvent and acetyl-acetone as stabilizing agent. The sol was simply 

dropped onto Au surface of sensor with comb-like structure, then dried at 110 °C in 

the oven and finally annealed at 450 °C in the calcination furnace. 

The obtained Au nanorods as well as the modified Au surface with TiO 2 structures 

were characterized by SEM. TiO 2 phase constitution and control of material 

purity were analyzed by Raman and ATR-FTIR spectroscopy, respectively. The electrical 

properties (resistance) of whole system were measured by impedance spectroscopy 

(IS). 

Keywords: gas sensor, TiO 2 , nanostructures


Posters 

5-P05 Image Analysis of Offset Lithography Prints 

Bohuslava Havlínová, Vladimír Dvonka, Ján Panák, Michal Čeppan 

Faculty of Chemical and Food Technology STU, 

Department of Graphic Arts Technology 

Radlinského 9, 812 37 Bratislava 1 

vladimir.dvonka@stuba.sk 

Reproduction of details on printing substrates is important from the point of 

view of the fidelity of image reproduction. Good quality printed image consists of 

screen dots of defined properties providing consistent pattern. 

In this contribution image analysis of screen dots were used to analyze two percent 

screen dot fidelity printed on different paper printing media by offset lithography. 

The front and back sides of set of coated and uncoated printing papers was 

tested. Micro- and macrostructure of the printed image on the printing papers were 

studied by image analysis – fractal analysis method. Micro structural parameters of 

screen dot size – area, perimeter, roundness, screen dot area and defects of screen 

dots – were evaluated. Missing screen dots, a macro structural image parameter, 

was evaluated too. 

The second part of image analysis based on print edge inspection. Print edge 

was analyzed by modulation transfer function and by fractal analysis. 

The set of printing papers was ranked from the point of view of quality described 

by the obtained micro and macro structural parameters. The quality of individual 

printing papers was correlated with physical properties of printing papers 

mutually one to another. 

Keywords: image analysis, fractal analysis, modulation transfer function, offset 

lithography, paper 

. 1


Posters 

5-P06 Color Fidelity of Reproduction 

of Historical Documents 

. 2 

Bohuslava Havlínová, Vladimír Dvonka, Michal Čeppan, Milena Reháková, 

Ján Panák 

Faculty of Chemical and Food Technology STU, 

Department of Graphic Arts Technology 

Radlinského 9, 812 37 Bratislava 1 

vladimir.dvonka@stuba.sk 

Preservation of cultural heritage is a very actual topic nowadays. Archiving of 

historical documents itself is based on restoration and digitalization of historical 

documents. Restoration is a difficult process involving mechanical and chemical operation 

and never can be regarded as forever solution. Therefore, a reproduction of 

historical documents, reliable from the point of view of carried information as well 

as appearance is in the centre of interest. Color fidelity is considered to be one of the 

most important aspects of reliable reproduction. 

This paper deals with color fidelity of reproduction process used for reproduction 

of historical documents. The quality of selected digital cameras and ink-jet 

printing papers was compared and evaluated from the point of view of provided 

color fidelity. CIE color differences were used as the key parameter. Based on the 

obtained results the system for reproduction of historical documents based on digital 

camera and ink-jet printer was designed and its operational performance was 

optimized. 

Keywords: color difference, color management, historical documents, 

color fidelity


Posters 

5-P07 Improvement of Barrier Properties of Paper 

Using Photochemically Modified Polymer Layers 

Viera Jančovičová, Sabine Amberg-Schwab 

Department of Printing Arts Technology and Photochemistry IPM, 

Faculty of Chemical and Food Technology SUT, 



The materials based on cellulose fibers (paper, card board) are sensitive to humidity 

and oxygen. The barrier properties of standard packaging materials are commonly 

achieved by lamination. An alternative to the lamination can be an introduction 

of the polymer coating on the paper surface. In this study, paper was coated by 

hybrid polymer with inorganic and organic structural units (ORMOCER ® ) and by 

polyvinylalcohol modified by glycidyl methacrylate (PVAGMA). 

The UV and sunlight curing process of ORMOCER ® and PVAGMA with various 

concentration of photoinitiator (Irgacure 2959 and Iragacure 184) were investigated 

and the compositions of both cured systems were optimised. Photopolymerization 

kinetic was evaluated on the basis of FTIR spectroscopy measurements. 

Two and three layer systems composed of paper, modified or unmodified polyvinylalcohol 

and ORMOCER ® were prepared and water vapor permeability, swelling 

and water sorption of these systems were studied. Deposited coatings (layers) 

significantly improved the barrier properties. 

Keywords: modified polyvinylalcohol, ORMOCER ® , barrier properties, 

photopolymerization) 

.


Posters 

5-P08 Influence of Ageing on Optical Properties of 

Arylmethane Dyes 

. 

Viera Jančovičová, Miroslava Petrovičová, Bohuslava Havlínová, 

Department of Printing Arts Technology and Photochemistry IPM, Faculty of Chemical and 

Food Technology SUT, 



Arylmethane dyes represent one of the oldest man-made dyestuffs used until 

today. This work deals with the characterization of dyes in solution by UV-Vis spectroscopy 

and degradation processes of the system dye-paper substrate by accelerating 

ageing studied by the reflectance UV-Vis spectroscopy and colorimetry. Six 

arylmethane days, one phenothiazine and one xanthene dye were investigated and 

spectral and colorimetric characteristics of them during ageing are discussed. 

The colourfastness and colour changes of these dyes applied onto an acidic 

paper support after conservation treatment (fixing and deacidification) and after accelerated 

ageing were followed and evaluated. Sandofix WE and cyclodecane were 

used as fixatives. Deacidification treatment was performed in water using magnesium 

and calcium bicarbonates. It is impossible to define one universal fixative, 

which would be appropriate for all kinds of the used dyes. 

Keywords: arylmethane dyes, UV-Vis spectroscopy, deacidification, fixation, 

optical properties

5-P09 Investigation of Decomposition of 

Photoinitiators 

Bohumil Jašúrek, Tomáš Syrový, Jan Vališ, Tomáš Weidlich 

University of Pardubice, Faculty of Chemical Technology, 

Department of Graphic Arts and Photophysics 


bohumil.jasurek@upce.cz 


Posters 

In this paper were investigated properties of four photoinitiators. Two of them 

are commercially available initiators (Irgacure 2959 and Irgacure 261) and the other 

two are new photoinitiators that were prepared (CB and HP1). CB is cationic organometallic 

photoinitiators and HP1 is hybrid photoinitiator with higher molecular 

weight containing chemical groups suitable for starting free radical and cationic 

polymerization simultaneously. 

The absorption properties and comparison of the rate of photodecomposition of 

tested photoinitiators were measured based on UV-VIS spectrometry. The photochemical 

mechanism and the estimated number of decomposition products after UV photolysis 

of initiators were proposed based on the results of mass spectrometry and factor analysis 

of UV-VIS spectra. The results from mass spectrometry and factor analysis have shown 

very good correlation. CB and HP1 decompose upon UV light exposure and can be used 

as photoinitiators for cationic (CB and HP1) or free radical polymerization (HP1). 

Keywords: photoinitiator, UV-VIS spectroscopy, MS, Factor analysis 

.


Posters 

5-P10 Comparison of the Photocatalytic Properties of 

TiO 2 and Au/TiO 2 in the Degradation of C 2 H 2 

. 

E. Puzenat, C. Youssef, N. Jaffrezic-Renault, J.-M. Herrmann, C. Guillard a 

Institut de recherches sur la catalyse et l‘environnement de lyon (IRCELYON), 

2 Avenue Albert Einstein 69629 Villeurbanne cedex France; Université Lyon 1, France. 

eric.puzenat@ircelyon.univ-lyon1.fr 

Photocatalysis over semiconductor metal oxides has received much interest in 

recent decades since it finds a lot of applications for the elimination of organic pollutants. 

Even if, in all cases, the complete mineralization of the initial molecule is 

reached, the chemical pathway shows many chemical intermediates. Recently, it has 

been established that the photocatalytic oxidation of acetylene over TiO 2 leads to the 

formation of CO in the gas phase for which the photocatalytic oxidation is a rather 

slow reaction. On the other hand, Au/TiO 2 catalysts exhibit an activity for the oxidation 

of CO at room temperature even without participation of UV light. 

Based on these two considerations, the purpose of the present work is the comparison 

of the photocatalytic oxidation of C 2 H 2 over TiO 2 and Au/TiO 2 . The comparison 

of the results obtained with the two samples (Fig. 1) shows that the disappearance 

of C 2 H 2 is faster with TiO 2 than with Au/TiO 2 but is accompanied with the 

formation of CO only for TiO 2 photocatalyst. This observation suggests that CO is a 

by-product of the photocatalytic oxidation of C 2 H 2 over TiO 2 and that is not formed 

when gold particles are present. 

The influence of the initial concentration of C 2 H 2 on the photocatalytic degradation 

rate was studied. The Langmuir-Hinshelwood model was used to describe 

the kinetic mechanism of the oxidation. A lack in the carbon balance 

(C 2 H 2 + CO 2 + CO) during the photocatalytic degradation suggests that some 

organic by-products are formed. A hypothesis explaining the different behaviour 

of the two samples lays down on the different kinetic for the oxidation of 

CO. In order to verify this, the photocatalytic oxidation of CO was study with 

the two samples. It clearly appears that the oxidation of CO is much faster over 

1 wt%Au/TiO 2 than over TiO 2 even without UV Irradiation. The Langmuir-Hinshelwood 

model was also used to describe the kinetic model. 

Finally, the photocatalytic oxidation of C 2 H 2 was studied using some mixtures 

(x 1wt%Au/TiO 2 //(1-x)TiO 2 ) with different x. The mixture showing the faster C 2 H 2 

oxidation without the formation of CO was 25%Au/TiO 2 //75%TiO 2 .. 

The extend of this abstract exceeded the allowed size and therefore had to be clipped.


Posters 

5-P11 Study of Influence of Iron-gall Inks Composition 

on Properties of Historical Documents 

Milena Reháková, Michal Čeppan, Vladimír Dvonka 

Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology 


milema.rehakova@stuba.sk 

The aim of work was experimental study of changes of paper containing iron 

gall inks with different composition. Ratio iron to gallic acid varied from 0.25 to 4. 

The manner of application and amount of inks were optimized. 

Viscosimetric determination of the average degree of polymerization of cellulose 

and calculation of speed constant of degradation reaction, thermal analysis of 

samples, colourimetric measurements, identification of inks by Falce Colour Infrared 

method and spectral analysis – in the range VIS – NIR were executed before and 

after the accelerated ageing. 

The iron gall inks with ratio iron:gallic acid greater than 0.67 effected on paper 

base very destructive. Abundance of iron in inks has negative effect. 

Keywords: iron gall inks, ageing 

.


Posters 

5-P12 The Influence of Receiving Layer Composition on 

Inkjet Print Lightfastness 

. 




xcstancik@fch.vutbr.cz 

Quality and properties of inkjet printouts are dependent on many factors: type 

of paper, composition of inks, properties of receiving layer, etc. In this paper attention 

was focused on the influence of receiving layer composition upon UV irradiation 

on lightfastness of inkjet printouts. The effects of inorganic pigment filler (TiO 2 ) 

and light stabilizer addition in layers of PVAL were studied. 

Amount of added filler is responsible for changes in print quality and its type 

has influence on light stability of printout. Light stabilizer addition should improve 

the lightfastness of samples. 

Specimens were printed by dye-based and pigment-based inkjet inks. Changes 

in the lightfastness were evaluated by total colour difference measurement. Metalhalide 

lamp was used as the source of UV irradiation. Light stability data for measured 

specimens are discussed. 

Keywords: poly(vinylalcohol), titanium dioxide, light fastness, colour diference.


Posters 

5-P13 Photopolymerization of Monomers with two 

Different Reactive Groups 

Josef Svoboda, Josef Knobloch 

University of Pardubice, Faculty of Chemical Technology, Department of Graphic Arts and 

Photophysics, 


josef.svoboda@upce.cz 

UV photopolymerization of three bifunctional monomers bearing epoxy and 

vinyl groups was studied. Monomers used were glycidyl methacrylate (GMA), glycidyl 

vinyl ether (GVE) and 3,4-epoxy-1-butene (butadiene monoxide, BMO). The 

homopoly-merization experiments were carried out at room temperature using 2isopropylthioxanthone 

(ITX) and/or (4-methylphenyl)[4-(2-methylpropyl) phenyl] 

iodonium hexafluorophosphate (IC250) as photoinitiators. Fourier transform infrared 

(FTIR) spectroscopy was used to follow the functional groups conversion during 

polymerizations. The transmission, the attenuated total reflectance (ATR) and 

the specular reflection techniques were applied. 

During the free radical (ITX) initiated photopolymerization the double bond 

shows higher reactivity within GMA than BMO, whereas the GVE conversion was 

negligible. Using the transmission technique, in the presence of IC250 the epoxy 

group conversion rate is much higher within GVE or BMO compared to GMA. With 

GMA photopolymerization monitored by the ATR technique both the reaction rate 

and the conversion maximum are considerably higher in comparison with the photopolymerization 

studied by the transmission or specular reflection techniques. 

Keywords: photopolymerization, bifunctional monomers, epoxy group, 

vinyl group, conversion rate, FTIR 

.


Posters 

5-P14 A new Aproach to Inkjet Prints Lightfastness 

Evaluation 

. 0 

Michal Veselý, Petr Dzik, Jiří Stančík 



vesely-m@fch.vutbr.cz 

At present, the quality of inkjet prints does not represent a technological limitation. 

But there is one problem still remaining: archival stability of these prints. 

Inkjet prints, especially photorealistic ones, are affected by environmental conditions 

during their storage or presentation. Absorption of visible and UV light can 

lead to dye excitation and reactive oxygen radicals formation usually followed by 

dye decomposition. This is observable as colour fading. 

This work deals with the study of lightfastness of selected photopapers designed 

for printing of photographs by inkjet technique. Four kinds of papers were 

used: glossy papers – Ilford Galery Smooth Gloss and Ilford Galery Clasic Gloss 

and matt papers – Epson Archival Matt and Foma 1224. The protective properties 

of laminating sheet on printed papers were studied. The lightfastness was studied 

by method of accelerated ageing in Q-Sun Xenon Test Chamber on target printouts. 

The colour differences of each colour field of target were measured by spectrophotometer. 

The colour differences ∆E ab * in course of accelerated ageing for all selected 

papers were evaluated. A new approach to lighfastness results evaluation is based 

on the use of a special printed target and on colour gamut volume calculation. 

Keywords: accelerated ageing, inkjet

Section 6: 

Physical and Applied Chemistry 

.1

Lectures 

.

Section – Physical & Applied Chemistry 

Lectures 

6-L01 Self-Aggregation of Humic and Fulvic Acids 

Studied on IHSS Standards 

Martin Drastík, Anna Čtvrtníčková, Jiří Kučerík 

Institute of Physical and Applied Chemistry, Faculty of Chemistry, 



xcdrastik@fch.vutbr.cz 

Humic substances are ubiquitous and also the most occurring organic matter 

on the Earth. Due to their high heterogeneity and chemical character they tend to 

self-aggregate forming various molecular organisations. Despite an intensive research, 

the mechanisms and ways of aggregation are still not precisely known. 

The aim of presented work is to bring new insights into aggregation mechanisms 

of several humic substances standards obtained from the International Humic 

Substances Society. The behaviour of sodium salts of humic and fulvic acids was 

investigated employing the high resolution ultrasonic spectroscopy (HRUS) which 

has become a powerful tool in colloidal chemistry due to its sensitivity to monitor 

any conformational change in a solution under study. Observed changes can be 

used to estimate the character of processes in progress. 

Keywords: humic substances, aggregation, HRUS 

.


Lectures 

6-L02 Robust Nonlinear Control of a Separation 

Column for 13 C Enrichment by Cryogenic 

Distillation of Carbon Monoxide 

. 

Eva-Henrietta Dulf, Clement Festila, Francisc Dulf 

Technical University of Cluj-Napoca, Faculty of Automation and Computer Science 

Romania, 400020 Cluj-Napoca, C. Daicoviciu Str., 15 

Eva.Dulf@aut.utcluj.ro 

In the present developing plan to apply the cryogenic technology for the production 

of the 13 C, an efficient and safe operation is a strong reason to conceive and 

to apply a modern computer based control strategy. The authors are concerned with 

the problem of developing effective and readily implemental techniques for modelling 

and control of the isotope separation plant. These columns are characterized by 

complex nonlinearities, with large time-delays. Furthermore, are subject to external 

disturbances, which are difficult to model. The controller synthesis problem for 

such processes is extremely challenging and has been an active area of research for 

the past two decades. In the present paper are studied two possible versions of robust 

nonlinear control of the separation column: the linearization approach and the 

synthesis of a robust nonlinear state feedback controller. The first approach is a mul- 

timodel controller design procedure, based on a mixed H 2 /H ∞ problem to achieve 

good quadratic performance and robustness. The control problem is defined so as 

to achieve optimal LQ (Linear Quadratic) performance subject to robust stability for 

the set of uncertain models. The second approach considers a single input-single 

output nonlinear differential difference equation system with uncertain variables. 

Keywords: isotope separation column, robust control


Lectures 

6-L03 Molecular Characterisation of Lignite Humic 

Acids by Chemical Degradations 

Laurent Grasset, Dana Válková, André Amblès 

Synthèse et Réactivité des Substances Naturelles, UMR CNRS 6514 

Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers, France 

laurent.grasset@univ-poitiers.fr 

The objective of this work was to specify structural molecular units of humic 

acids extracted from South Moravian lignite. The primary structure of humic acids 

has been studied using chemical degradations such as thermochemolysis, RuO 4 and 

H 2 O 2 oxidations. 

TMAH and TEAAc thermochemolysis are able to discriminate between total 

(bond and/or trapped), trapped and directly extractable hydrophobic compounds. 

These compounds were mostly fatty acids and were in a “free” form. 

Chemical oxidations resulted in an extensive degradation/solubilisation of the 

humic acids and the hydrophilic compounds largely dominated the oxidation digest 

in the case of both RuO 4 and H 2 O 2 . Relatively low amount of benzene(poly)carboxylic 

acids indicated only a limited content of fused aromatic moieties. The identification 

of traces of saccharides confirmed the low maturity of the studied sample. The 

major compounds present in the oxidation digests are short chain (poly)carboxylic 

acids. They are usually considered to be the result of the oxidation of carbohydrates. 

They could be also the result of the oxidation of the short aliphatic chains between 

aromatic and/or oxygenated moieties in ligneous network. 

Chemical degradations are able to give informations on the molecular structure 

of such complex natural organic matter. On the other hand, humic acids extracted 

from South Moravian lignite are able to release, when oxidized, high amount of 

hydrophilic short chain (poly)carboxylic acids. 

.


Lectures 

6-L04 Prediction of the Acetic and Formic Acid 

Formation in the Paper During the Accelerated 

Ageing by the Change of Optical Properties 

. 

M. Jablonský, K. Hroboňová, J. Lehotay, S. Katuščák 


Faculty of Chemical and Food Technology, 

Slovak University of Technology in Bratislava, Radlinskeho 9, 

SK-812 37 Bratislava, Slovak Republic. 

michal.jablonsky@stuba.sk 

Natural ageing of all cellulose-based papers including alkaline papers is attended 

by spontaneous production of organic acids. In principle, simple acid hydrolysis 

does not affect colour, but low-molecular-weight products are more prone to oxidation 

and colour formation than cellulose chains. The change of optical properties is 

results of paper degradation. The paper properties are interdependent. Carboxylic 

acids presented in the paper intensify force of degradation and thus increase its rate. 

The obtained results show that during accelerated ageing at the temperature 98 °C, 

acetic and formic acid are being produced in unmodified papers. The multiple linear 

regression model was used to describe the relationship between concentration of 

acetic acid (C(AA)) and independent variables such as time of accelerated ageing (t) 

and Kubelka-Munk coefficient (k/s). The equation of the fitted model is 

C(AA) = 1.0985 + 0.2119.t –3.6854.k/s. The r 2 parameter indicates that the model 

as fitted explains 96.70% of the variability in acetic acid content. For concentration of 

formic acid, the equation of the fitted model is C(FA) = 0.299+ 0.0209.b*. The r 2 parameter 

indicates that the model as fitted explains 34.40 % of the variability in formic 

acid content. Low-molecular product such as acetic acid, have coherence with the 

colour information which is consequence of the accelerated ageing. 

Keywords: optical properties, acetic acid, accelerated ageing


Lectures 

6-L05 Effect of Modification of Paper with 

Antioxidant of Piperidine Serie on 

Recognisability of Black Characters on Paper 

Jana Kazíková, Attila Szitás, Katarína Vizárová, Michal Jablonský, 

František Považanec, Soňa Kirschnerová, Svetozár Katuščák 

Institute of Polymer Materials, Department of Chem. Technology of Wood,Pulp and Paper, 


Radlinského 9, 812 37, Bratislava, Slovak Republic 

E-mail: jana.kazikova@stuba.sk 

Colour of paper is changed both by ageing and conservation processes and this 

causes the letters and other optical characters visibility and recognisability changes 

on the paper (R S ). It is generally believed that R S of a optical characters in document 

depends on the contrast between text character and a background (∆E C-B ). The aim 

is to estimate effect of paper ageing and modification on the R S . Paper samples 

were modified by the 0,5 % hexamethyldisiloxane solution of piperidine antioxidant 

DAO3 , and aged in hermetically sealed glass tubes at 98 o C. The kinetics of 

paper optical properties were measured and the R S was calculated by equation R S = 

k 1 .(k 2 . (∆E C-B ) 2 – k 3 . ∆E C-B + k 4 ). The relative decrease of the colour direfence between 

a model character and the real paper background caused by the accelerated ageing 

after 360 hours was ∆(∆E* C-B ) = -8,3%, the equivalent R* S = -1,1% for the nonmodified 

paper, while for the antioxidant modified paper the ∆(∆E* C-B ) = -11,8% and the R* S 

= -2,4%. The impact of ageing on the ∆(∆E* C-B ) of on dark paper with the lightness 

L* = 45 or the dark parts of documents, from ∆E C-B = 15, is much more meaningful, 

with the R* S = -16,7%. The decrease of the optical characters recognisability R* S is 

insignificant on the white papers while much more significant on the dark papers or 

the dark parts of document. The piperidine derivative antioxidant DAO3 used have 

negative effect on the recognisability decrease. 

Keywords: visibility, recognisability, optical characters, letters, antioxidant, 

paper, documents, ageing 

.


Lectures 

6-L06 Role of Aromaticity in Humic Substances 

Degradation Kinetics Using 

Non-Arrhenius Temperature Functions 

.10 

Jiří Kislinger, František Novák, Jiří Kučerík 




xckislinger@fch.vutbr.cz 

Actual worldwide environmental problems as climate changes imply reduction 

of greenhouse gases. Such process demands fundamental understanding of 

carbon (C) stabilization in soils. The mechanisms for C stabilization are still not well 

understood and thus the ultimate potential for C stabilization in soils is unknown. 

The main fraction of organic matter contained in soils and other natural materials 

consists of humic substances, which comprise a complex mixture of both aromatic 

and aliphatic moieties, having a large number of functional groups. 

To describe the kinetics of processes in condensed phase, the methods based 

on the single-step approximation are often used. For our purposes isoconversional 

methods are highly suitable. The temperature function in general rate equation 

needn’t necessarily be the known Arrhenius one and thus two other functions were 

used. 

The aim of the work lies in elucidation of role of aromatic carbon content during 

the humic substances thermo-oxidative degradation. The results could help in 

assessments of soil organic matter erosion or in development of remediation and 

soil restoration techniques. 

Keywords: humic substances, kinetics, isoconversional methods


Lectures 

6-L07 NMR Spectroscopy of Minor Compounds 

in Wine – a Comparison of Different 

Pre-Concentration methods 

M. Mazur, K. Furdíková, M. Kaliňák, M. Valko, V. Žúbor, N. Prónayová 

Faculty of Chemical and Food Technology, Slovak University of Technology, 

Radlinského 9, SK-812 37 Bratislava, Slovak Republic; 

e-mail: milan.mazur@stuba.sk 

Wine is a complex mixture consisting of several hundreds substances present 

at different concentrations. The dominant ones are water (86 %), ethanol (12 %), glycerol 

(1 %), sugars, organic acids, and various ions. The other aliphatic and aromatic 

alcohols, amino acids and phenol compounds are present at much lower concentrations 

(


Lectures 

6-L08 Hydrophobization of Paper by Vapours in N 2 

Plasma at Atmospheric Pressure 

.12 

Milan Mikula, Alexandra Valaseková, 

Zuzana Jakubíková, Viera Jančovičová 

FCHPT STU, 812 37 Bratislava 

milan.mikula@stuba,sk 

Standard volume dielectric barrier discharge (DBD) and down stream (after 

DBD) discharge at atmospheric pressure were applied for continuous treatment of 

paper, using nitrogen as working gas with several reactive organic or organosilicon 

vapors (including hexamethyldisiloxane – HMDSO and n-heptane). 

Modified hydrophobized papers were characterized by wettability, surface energy, 

water imbibition (soaking), sorption and penetration properties and kinetics 

and by absorption FTIR spectroscopy (surface sensitive ATR techniques). 

The level of hydrofobization of paper in different vapours will be discussed 

depending on conditions and on the manner of applying the vapours, paper directly 

in the standard DBD plasma of gas/vapour mixture or in down stream after glow 

DBD. 

Keywords: hydrophobization, paper, plasma


Lectures 

6-L09 Fluorescence Study of Polysaccharide in Dilute 

Aqueous Solution 

Filip Mravec, Tereza Halasová, 

Miloslav Pekař, Vladimír Velebný 

CPN Ltd & IPAC FCH BUT 

Dolní Dobrouč 401, 561 02 Dolní Dobrouč, CZ & Purkyňova 118, 612 00 Brno, CZ 

mravec@fch.vutbr.cz 

Aggregation properties of sodium hyaluronate (HA) in different molecular 

weights were studied by fluorescence methods. Interactions with hydrophobic fluorescence 

probes showed sodium hyaluronate, or its hydrophobically-modified analogues, 

as suitable systems for drug delivery applications. Properties of hydrophobic 

core were studied with polarity and viscosity fluorescence probes. 

Interaction with hydrophilic probes, e.g. acridine orange or “styryl-class” 

probes, can help to understand hyaluronate’s self-aggregation behavior in aqueous 

environment. Depolymerization of dye-aggregates, evidenced by fluorescence intensity, 

showed an abrupt change in physiological concentration region (~ 1 g L -1 ). 

Keywords: fluorescence probe, hyaluronic acid 

.1


Lectures 

6-L10 Spin-Trapping Investigation of Radical 

Intermediates Generated from Phenols. 

Specific Behaviour of Para-Methyl Phenols 

.1 

Ladislav Omelka, Petr Majzlík, 

Renata Superatová, Petra Holubcová 



omelka@fch.vutbr.cz 

Phenoxyl radicals, generated from the series of substituted phenols and bisphenols 

were indirectly detected using nitroso spin traps. The active site for the 

addition of phenoxyl to nitroso group strongly depends on the substitution. In the 

case of unhindered phenols the addition proceeds in the ortho position of the corresponding 

phenoxyl, sterically hindered phenoxyls are added mostly through their 

para position. In para methyl substituted phenols the tendency towards the abstraction 

of hydrogen from para methyl substituent leading to the formation of benzyl 

radicals was observed. Their adducts with nitroso spin traps undergo the consecutive 

transformation to the nitrones. 

Keywords: EPR spectroscopy, phenoxyl radicals, spin trapping


Lectures 

6-L11 Vortex-Fractal-Ring Structure of Molecule 

Pavel Ošmera 


Technicka 2, 616 69 Brno, Czech Republic 

osmera@fme.vutbr.cz 

This paper is an attempt to attain a new and profound model of the nature’s 

structure using vortex, fractal and ring structures. Scientists try to explain some 

phenomena in Nature that have not been explained so far. The aim of this paper is 

the vortex-fractal modeling of vortex-ring fractal structure of atoms, molecules, and 

a creation of elements in the Mendeleev’s periodic table with vortex-ring particles 

which is not in contradiction to the known laws of nature. 

Fractals seem to be very powerful in describing natural objects on all scales. 

Fractal dimensions and fractal measures are crucial parameters for such description. 

Many natural objects have self-similarity or partial-self-similarity of the whole 

object and its part. 

Our science creates terrific demands on the imagination. To understand the 

electromagnetic field requires a high degree of imagination. The degree of imagination 

that is required is much more extreme than that required for some of the 

ancient ideas. The modern ideas are much harder to imagine. We usually use mathematical 

equations and rules, but we used a lot of pictures to describe very complex 

vortex-fractal models. There is not easy to formulate mathematical models. We can’t 

allow ourselves to seriously imagine things, which are obviously in contradiction 

to the known laws of nature. We created vortex-fractal-ring model of the electron, 

of the proton and hydrogen and then calculated sizes, forces, energies and covalent 

bonds. 

Keywords: vortex-fractal structures, covalent bond 

.1


Lectures 

6-L012 Hydration of Hyaluronan 

.1 

Alena Průšová, Petra Bursáková, Jiří Kučerík 


Department of Applied and Physical Chemistry 


xcprusova@fch.vutbr.cz 

Hydration is one of the most important factors playing the key role in biological 

function of molecules in living systems. Water represents an important medium 

for nutrient transport, cell membrane processes, induces biologically active conformation 

of biomolecules etc. Due to unique properties and extremely high affinity of water 

molecules to each other it forms various structures (clusters) having different physicalchemical 

properties. 

Hyaluronan is naturally occurring high molecular weight biopolymer consisting of 

glucuronic acid and N-acetyl-glucosamine. One of the most important functions of the 

hyaluronan is hydration (the hydration of epidermis or the hydration of ocular cornea). 

Hyaluronan binds water very strongly but there are some physical differences between 

the structures of binding water. 

The aim of this work was to study hydration properties of hyaluronan. The enumeration 

of water molecules is crucial in order to understand how biomolecular processes 

work. The ability of various hydration shells to freeze and melt in broad temperature 

range was used to determine these different types of water using the DSC 

method. There were recognized three types of water ‘bulk water’ which freezes at the 

same temperature as normal water, ‘freezing-bound’ water which freezes at a lower 

temperature than normal water and it also exhibits a reduced enthalpy of fusion and the 

‘non-freezing’ water which does not freeze even at low temperature. 

Keywords: hyaluronan, hydration, DSC


Lectures 

6-L13 Electrochemical Analysis of New Indole-Based 

Antioxidants 

P. Rapta, M. Zalibera, V. Šnirc, S. Štolc 




peter.rapta@stuba.sk 

The redox properties of stobadine (Stobadin.2HCl) and new series of pyrido-indole 

derivatives (SMe1EC2.HCl, SMe1M3EC2.HCl, SM1M3EC2.HCl, SMe1–iProC2. 

HCl, SMe1–iBuoC2.HCl and SMe1–nBuoC2.HCl) were investigated by cyclic 

voltammetry both in non-aqueous (DMSO) and aqueous solutions. Electrochemical 

and EPR/spin trapping investigations of new synthesised pyridoindoles were 

performed in order to explain in more detail the redox processes of investigated 

compounds in relation to their antioxidant and radical scavenging activity. Antioxidant 

capacity was determined by three independent methods for evaluation of 

antioxidant activity including ABTS and DPPH tests as well as EPR spin trapping 

assay. Cyclic voltammetric and EPR studies indicate different oxidation products 

depending on the pyridoindole substitution and on the solvent used. 

Keywords: cyclic voltammetry, stobadine derivatives, EPR spectroscopy, 

follow-up reactions, oxidation 

.1


Lectures 

6-L14 New Colloidal Systems made of Humic Acids 

.1 

Petr Sedláček, Martina Klučáková, Miroslava Malenovská 



sedlacek-p@fch.vutbr.cz 

Humic acids represent complex natural material, which shows many valuable 

physical and chemical properties. Because of high sorption ability it contributes to 

the native self- detoxification of soil and sediments and also provides the promising 

substrate for an artificial production of soil remediation agents, industrial sorbents 

etc. Humic acids are gained from their rich alternative natural sources – peat, 

young coal etc. – by simple and cheap methods of extraction and purification in a 

form of fine powder soluble in alkalines. Hence, numerous papers focus on a study 

of humic acids powder or humate solutions. Nevertheless, none of these forms is 

suitable for the direct application in agriculture or industry. Submitted contribution 

introduces several laboratory procedures developed for the preparation of new humic 

acids -based colloids (hydrogels, flakes and beads) with the discussion of their 

applicability. 

Keywords: Humic acids, hydrogels


Lectures 

6-L15 Photochemical and Free Radicals Study of 

Cyanobacteria using EPR Spectroscopy 

Stopka P., Maršálek B., Křížová J. 

Institute of Inorganic chemistry v.v.i., Czech Academy of Science, 

25068 Husinec-Řež 

stopka@i ic.cas.cz 

Our previous experiments measured by electron paramagnetic resonance spectroscopy 

(EPR, ESR) proved, that actively growing cyanobacteria produce characteristic 

spectrum of stable radicals, which may play an important role in aquatic 

ecosystems. Our preliminary experiments analysed by EPR also proved, that barley 

and wheat straw produce quite different spectrum of free radicals, Application of 

barley straw was published many times, but the principle remains unknown. At 

least on of he principle could be based on the trapping and quenching the cyanobacterial 

stable radicals by specific radicals produced by especially prepared natural 

materials. This „neutralisation“ of radicals could be safe for aquatic ecosystems. 

We plane to select different natural plant materials with potential algistatic activity 

Our aim is to prepare from these materials potential algistatic fractions after specific 

initialisation processes (fermentation, rotting etc.) and to define their ability of free 

radicals production. Principal question of this project of basic research is to prove, 

if above mentioned mechanism is really responsible for the selective interaction of 

cyanobacteria with barley straw. Second aim of the project is to compare spectrum 

of free radicals produced by barley straw and compare them with another natural 

materials, which can be nominated as more selective and even more practical tool 

for cyanobacterial blooms control than the barley straw is. 

Eutrophication of aquatic ecosystems and the consequent mass occurrence 

of harmful cyanobacterial blooms are growing problems in the entire world. Cyanobacterial 

blooms deteriorate water quality by toxins and odours production. 

Cyanobacterial toxins (microcystins, anatoxins, lipopolysacharide etc.) are known 

to have hepatotoxic, neurotoxic, imunotoxic, genotoxic and other adverse effects. 

Cyanotoxins in drinking water, fish consumption and recreational use of reservoirs 

represents a growing risk for human health. Besides this, cyanobacterial bloom 

means considerable stress for ecological stability of aquatic ecosystems, natural fish 

and amphibian reproduction, biodiversity of phytoplankton and zooplankton community 

etc. Not only toxin production is harmful, but also decrease of light intensity, 

pH change and excessive oxygen depletion. Therefore measures ... 


.1


Lectures 

6-L16 Molecular Electronic: Advances and Limitations, 

Strategies, Materials, Methods and Applications 

.20 

Martin Vala, Martin Weiter, Oldřich Zmeškal, Ota Salyk 



vala@fch.vutbr.cz 

Over the past several decades there have been dramatic advances toward the 

realization of electronic components from organic materials instead of the inorganic 

semiconductors. The initial simple replacement of the traditional materials to costeffective, 

but usually less performing, organic counterparts was superseded to nature-inspired 

materials and recently to completely new specially designed ones. 

However, the true advancement is achieved by utilizing of the original properties 

of the organic materials to construct new devices based on those individual 

features which do not have analogies in the inorganic semi-conducting physics. This 

is usually accomplished with the help of quantum chemical calculations which can 

predict the desired properties of the new materials prior to the synthesis. 

The aim of this lecture is to introduce some of the strategies employed at our 

department and to show the development achieved. The attention will be also paid 

to designed novel materials particularly suitable for optoelectronics as exemplified 

by displays, photovoltaics and optical memories. 

Keywords: molecular electronics, organic electronics


Lectures 

6-L17 Mutual Relation between Fractal and Statistical 

(Random, Thermodynamic) Phenomena in Nature 

Oldřich Zmeškal, Martin Weiter, 

Martin Vala, Tomáš Bžatek 


Faculty of Chemistry, Purkynova 118, 612 00 Brno 

zmeskal@fch.vutbr.cz 

This paper deals with the unification of views of different physical and chemical 

fields of knowledge on natural phenomena. We put into context physical quantities 

defined in fractal physics, thermodynamics and statistical physics with general 

mathematical description by momentum of random quantities. 

The mathematical tools of fractal physics enable a universal description of such 

phenomena as e.g. thermal radiation, conduction and convection of heat, properties 

of ideal and real liquids and/or gases, thermodynamic entropy of closed and open 

system, classic and quantum distribution functions. 

This unification in not only of a theoretic importance, but enabled the implementation 

of further modules into the application HarFA (developed by authors of 

this paper). The program HarFA was intended for fractal analysis of digital images, 

video clips, signals and numerical data sets. The new modules enabled the calculation 

of five fractal dimensions (topological, information – thermodynamic, correlative, 

skewness and kurtosis) which relate to corresponding moments and entropies. 

Thus a unification of phenomena description by different sciences is achieved. 

Keywords: fractal physics, entropy, statistical physics, thermodynamics 

.21

.22

Posters 

.2


Posters 

6-P01 Influence of Regenerated Humic Acids on 

Polyvinyl Alcohol Thermo-Oxidational Stability 

Barbora Bakajová, Zoja Vlčková, Jiří Kučerík 


Purkyňova 464/118, 612 00 Brno 

xcbakajova@fch.vutbr.cz 

Using of natural antioxidants is necessary for the future sustainable way of 

life since the availability of oil is slowly decreasing. The technological potential 

of lignite as a chemical raw material has been up to now overlooked although it 

represents an interesting and unique natural product rich in humic acids content. 

Humic acids consist of a mixture of different organic molecules which major pool 

represents unsaturated fatty acids and polyphenols. As it has been demonstrated 

recently, added to a polymer, humic acids can be used for adjusting of its stability 

due to their profound antioxidant effect. Oxidation of parental lignite leads to formation 

of so-called regenerated humic acids which offer a wide range of properties 

reflected usually by the content of functional groups and/or by variable aromaticity. 

Thus, application of regenerated humic acids as additives in polymers can be a way 

to develop a green polymer with predictable stability and (bio)degradability. Such 

aspect can be crucial in selection of materials useful in agricultural as well as in environmental 

applications where the quick and easy degradability of a polymer after 

its expiration time is often requested. 

In this work we have focused on the oxidation stability evaluation of polyvinyl 

alcohol/regenerated humic acids blends using thermogravimetry. To assess the efficiency 

of antioxidant or pro-oxidant influence the isoconversional methods were 

used. 

Keywords: humic acids, polymer stability, thermal analysis 

.2


Posters 

6-P02 Determination of Particle Shape and Size 

Distribution 

.2 

Edita Bretšnajdrová, Ladislav Svoboda, Jiří Zelenka 

University of Pardubice, Faculty of Chemical Technology 

nam. Cs. Legii 565, 532 10 Pardubice, Czech Republic 

edita.bretsnajdrova@student.upce.cz 

At present great attention is given to study of preparation and properties of 

various nanomaterials usable in many applications. They are utilized in varied fields 

of human activity – e.g. in electronics, medicine, paint industry etc. Except detailed 

chemical structure, such nanoparticle properties as shape and size distribution are 

fundamental to the given application. To measure these parameters various methods 

are used, e.g. transmission electron microscopy (TEM), atomic force microscopy 

(AFM), acoustic spectrometry and methods based on the light scattering. 

All above-mentioned methods were used in this work to characterize particles 

of two selected model types of nanomaterials – colloidal silica and sodium montmorillonite. 

Silica represents a material with spherical particles, tabular shapes are 

typical for montmorillonite materials. 

Experiments proved that methods used to measure particle size distribution 

and based on the light scattering require very diluted dispersions to meet the needs 

of them. But, on the other hand, these conditions can affect the particle size distribution 

due to agglomeration or deagglomeration of them. For these reasons methods 

working with concentrated suspensions, without dilution, are more suitable to 

get correct results for such colloidal systems, for example acoustic spectrometry. To 

obtain information on the shape of particles studied TEM and AFM methods were 

used. 

Merits and limitations of the individual methods used to evaluation of nanoparticles 

of various types are discussed in the work. 

Keywords: particle size distribution, acoustic spectrometry, silica, 

montmorillonite, nanomaterials.


Posters 

6-P03 Hydration of Regenerated Humic Substances 

Considering their Biological Properties 

Petra Bursáková, Zoja Vlčková, Zdeněk Cihlář, Jiří Kučerík 


Institute of Applied and Physical Chemistry 


xcbursakova@fch.vutbr.cz 

Humic substances (HS) have a profound effect in many processes including 

cell biology of living organisms. As they are both hydrophobic and hydrophilic in 

nature, their function is closely related to the properties of water shell intimately 

bound on humic molecules and consequently on humic aggregates. A strong affinity 

of water molecules to stick to each other via H-bonds and formation of specific clusters 

is a driving force assembling humic molecules into complicated organizations. 

The differences in properties of water surrounding HS can be recognized using 

DSC. Phase transition behaviour of sorbed water in HS-water systems was investigated 

as a function of water content. Three types of water can be distinguished: free 

water whose melting temperature and enthalpy are not significantly different from 

those of normal (bulk) water, those water species exhibiting large differences in 

transition enthalpies and temperatures (freezing-bound water), or those for which 

no phase transition can be observed calorimetrically, known as non-freezing water. 

The aim of this work is to find a connection between hydration ratio of regenerated 

HS from various sources considering their biological activity which can bring new 

information regarding function of HS in biological processes. 

Keywords: humic substances, hydration, DSC 

.2


Posters 

6-P04 Evaluation of Sorption Abilities of Natural 

Lignite for Organic Substances 

.2 

Petra Bušinová, Miloslav Pekař 



e-mail: businova@fch.vutbr.cz 

South Moravian lignite has interesting chemical composition which allows its 

use in various non-fuel applications, especially in sorption processes. Submitted paper 

reports on lignite sorption abilities for basic textile dyes and petroleum products 

(gasoline, diesel fuel, oils), presents sorption capacities, isotherms and kinetics. Results 

show that lignite can be used as a sorbent for tested substances. Possibilities of 

practical application of natural lignite for organics sorption are discussed. 

Keywords: lignit, organic substances, sorption


Posters 

6-P05 Maximum Bubble Pressure and the du noüy 

Platinum Ring Method of Surface Tension 

Measurements of Sodium Dodecyl Sulfate and 

Sodium Hyaluronate 

Martin Chytil, Jitka Krouská, P. Kulilová, Miloslav Pekař 

Institute of Physical and Applied Chemistry, Fakulty of Chemistry, Brno University of Technology 


The comparison of two methods for measuring surface tension (γ), maximum 

bubble pressure (MBP) and Du Noüy platinum ring method (DNR), are presented 

and demonstrated on measurements of sodium dodecyl sulfate (SDS) and sodium 

hyaluronate (NaHA) in water and 0.15 M NaCl at room temperature. From the DNR 

method, the plot of γ against measuring time t meas is obtained for each measurement, 

whereas from the MBP method, the dependence of γ on the time life of the air bubble 

(t life ) is gained. The surface tension of a measured solution is defined as the equilibrium 

surface tension, γ eq , i.e. γ at infinite time, t ∞ . 

Clear differences between the two methods can be seen; the γ values obtained 

from DNR method are basically lower than those from the MBP method, particularly 

for SDS solutions. The differences result in a bit higher critical micelle concentration 

(CMC), and a steeper drop of γ before the CMC for the MBP method. The 

differences most likely arise from the fact that adsorption at the curved air/solution 

interface of the bubble is measured using MBP method instead of adsorption at the 

flat air/solution interface for the DNR method, which points out another balance 

of adsorption/desorption at the curved interface is established in the system and a 

different affinity and dynamics of the surfactant molecules to the curved interface. 

The differences were not so significant for NaHA solutions due to rather low surface 

activity of NaHA and mostly no systematic trend in the progress of γ with NaHA 

concentration was observed. However, there are small differences between higherand 

-lower-molar mass HA ((H)LMHA), showing slightly stronger surface activity 

of LMHA. 

.2


Posters 

6-P06 Surface Tension of Regenerated Humic Acids 

Salts 

. 0 

Anna Čtvrtníčková, Jiří Kučerík 



annactvrtnickova@seznam.cz 

Humic acids represent a versatile heterogeneous mixture of organic substances 

developed as decay intermediates of mostly photosynthesis products and which 

chemistry completely differs from that of chemically pure (homogeneous) substances. 

That is also a case of surface tension measurement. While pure substances exhibit 

a relatively simple record, i.e. equilibration is reached maximally within minutes, 

humic substances components tend to adsorb on the water surface within hours and 

consequently affect the surface tension of the solution. It is a consequence of both 

kinetics and thermodynamics contribution. In the former, also the contribution of 

diffusion processes should be taken into account. 

Processes included in changes of surface tension of humic material are worth 

to study from several points of view; i) they reflect the mechanism aggregation of 

humic molecules in the solution, ii) indicate the affinity of mutual interactions (repulsions/attractions) 

of humic molecules which has the influence on the biological 

activity and last but not least iii) deep knowledge can be technologically exploitable. 

In this study, surface tension measurements was carried out on a set of modified 

humic acids with the purpose to elucidate the mechanisms of processes occurring 

both on the surface and in the solution and to find technologically appropriate 

composition of humic acids useful for washing technology of highly polluted soils 

(i.e. remediation). 

Keywords: humic acids, surface tension, remediation

6-P07 Immobilization of Lignite into 

PVAl Sponges 

Petr Dzik, Václav Mach, Michal Veselý 



petr@dzik.cz 


Posters 

Lignite is from geological point of view the youngest form of fossil fuels. Therefore 

its energy content is rather low. However, much attention has been paid to 

non-energetic applications of lignite recently. Apart from other interesting way of 

utilization, lignite proved to be an efficient sorbent for a number of pollutants. 

Although loose finely groung lignite can be used on its own, it might be beneficial 

for a number of industrial application to use some immobilised form of lignite, 

for example for water purification. Therefore some process for lignite immobilization 

has to be found. 

Our work was dedicated to the immobilization of lignite into highly porous 

hydrophilic sponges made of acetalized PVAl. These sponges were prepared by acidic 

formalization of aqueous solution of PVAl. Pores were formed by gaseous H2, 

which originated from the reaction of powder aluminum with acid. Resulting sponges 

were of open-cell structure, were highly hydrophilic and had variable porosities 

depending on the amount of blowing agent (aluminum). 

A set of sponge samples with varying lignite content was prepared and the properties 

of resulting sponges were studied (free pore volume, swelling characteristics). 

We also studied the sorption capacity of lignite-containing sponges on model 

pollutants (Cu(II) ions and methylene blue). 

Keywords: lignite, immobilization, sponge, sorption 

. 1


Posters 

6-P08 FTIR and SFS Spectra of Humic Acids Isolated 

from Lignite and Chernozem 

. 2 

Naděžda Fasurová and Ľubica Pospíšilová 


Institute of Physical and Applied Chemistry, 

Purkyňova 118, 612 00 Brno, 

fasurova@fch.vutbr.cz 

In this paper comparison of chemical structure and optical properties of humic 

acids (HA) isolated from various sources is presented. Excellent sorption properties 

of HA are well known to depend on their chemical structure and composition. Therefore 

HA isolated from different matrices (soil, lignite) were studied using FTIR and 

SFS spectral methods. FTIR spectra contributed to authentic knowing of functional 

groups in HA molecule. The inherent fluorescence of HA is an extremely sensitive 

measure, which allows for non-destructive analyses of samples. Object of our study 

were HA isolated from lignite (locality Mikulčice) and Modal Chernozem (locality 

Bratčice). Isolation was made according to the international standard IHSS method 

and according to Czech standard on determination of content of humic substances 

in coal. FTIR spectra were measured using KBr technique with Nicolet Impact 400 

spectrometer. SFS spectra were measured by Spectrofluorimeter Aminco Bowman 

within the range 320 - 620 nm (at Δλ=20 nm). 

Keywords: FTIR spectra, SFS spectra, lignite HA, Chernozem HA


Posters 

6-P09 Enhancement of Northern Softwood Bleached 

Kraft Pulp Accessibility and Acid Hydrolysis by 

Means of Ultrasonic Treatment 

Michal Jablonský, Štefan Šutý, B. Švehla 

Department of Chemical Technology of Wood, Pulp and Paper, Slovak University of Technology 

in Bratislava, Radlinskeho 9, SK-812 37 Bratislava, Slovak Republic michal.jablonsky@ 

stuba.sk 

Northern softwood bleached kraft pulp were treated with ultrasonic in order 

to improve the accessibility and hydrolysis of cellulose. The influence of ultrasonic 

treatment on morphology structure changes and on accessibility were discussed. 

Results revealed an increase in cellulose’s accessibility in terms of water retention 

value (WRV) with increasing ultrasound treatment time with the different frequency 

25, 40 and 80 kHz of sonification treatment. Furthermore, the acid hydrolysis of 

cellulose has been also successfully improved by the ultrasound treatment. However, 

no significant changes in crystallinity of cellulose were noted after ultrasonic 

treatment which was characterized by means of FTIR. 

Keywords: FTIR, WRV, ultrasonic, crystallinity 

.


Posters 

6-P10 Modelling of Complexation of Heavy Metals And 

Humic Acids with Utilization of High Resolution 

Ultrasound Spectroscopy 

. 

Martina Klučáková, Jiří Kučerík 

Miloslav Pekař 




klucakova@fch.vutbr.cz 

Several model compounds (citric acid, hydroquinone, pyrocatechol, salicylic 

acid and EDTA), containing functional groups and structure similar to binding sites 

in structure of humic acids, were chosen for this contribution. Our previous results 

confirmed that these model substances can be very useful in study of complexation 

of humic acids with metal ions. High Resolution Ultrasound Spectroscopy (HRUS) 

is method gaining ground more and more in research of bio-colloids. Interactions of 

cupric ions with these models are studied by HRUS and obtained results are compared 

and discussed with previous ones measured by means of different methods. 

Keywords: ultrasound scpectroscopy, complexation


Posters 

6-P11 Kinetics of Sorption of Metal Ions on Lignitic 

Humic Acids 

Martina Klučáková 





Affinity of humic acids (HA) to heavy metals is well known. Many authors use 

copper as model metal for study of interaction with HA due to its high complexation 

ability and affinity to HA. My previous works showed that classical Langmuir isotherm, 

which is frequently used for mathematical description of equilibrium sorption 

of metal ions on HA and determination of sorption capacity, is connected with 

some inaccuracies, because it does not take into account the production of hydrogen 

ions during reactions of metal ions with acidic functional groups on the surface of 

humic particles. In view of the fact, that Langmuir isotherm is based on kinetics of 

sorption process, I develop new model for equilibrium sorption of metal ions on 

HA including splitting of hydrogen ions in chemical sorption on acidic groups. The 

model was proved experimentally for sorption of cupric ions. This work deals with 

kinetics of sorption of cobalt and nickel ions on HA to prove above mentioned new 

model for metal ions with lower affinity to HA. 

Keywords: humic acids, sorption 

.


Posters 

6-P12 Acid-base Properties of Fractionated Humic 

Acids 

. 

Martina Klučáková, Ondřej Pilný 





Humic acids (HA) were fractionated by their dissolution in universal buffers 

(NaOH, H 3 BO 3 , H 3 PO 4 and CH 3 COOH) with various pH-values. Obtained fractions 

were characterized by UV/VIS and FT-IR spectroscopy and their acid-base properties 

were studied. It was found that contain of acidic groups in majority of individual 

fractions is higher than the content of original HA samples. Our hypothesis is 

that HA structure is changed during fractionation. HA can interact with individual 

components of buffer and re-arranged their structure, which results in apparently 

higher acidity of obtained fractions. The re-arrangement of HA structure probably 

causes, that some acidic groups inside HA aggregates, which are not able dissociate 

in original HA sample, can contribute to measured acidity in individual fractions. 

Surprising result is that residue after dissolving original HA in very alkaline buffer 

(pH = 12) has after fractionation (and “exhaustion” of the largest amount of HA 

fraction) several times higher solubility in water as others. On the other hand, this 

fact corresponds with our hypothesis of re-arrangement and possible interactions 

with buffer, because the most “aggressive” buffer caused the most marked changes 

in HA properties. 

Keywords: humic acids, fractionation, acid base


Posters 

6-P13 HF and DFT Calculations of the Infrared 

and Raman Spectra of Lanthanide Complexes 

Cp* 2 LnCl 2 Li(OEt 2 ) 2 , where Ln = Sm, Nd 

Ondřej Kroutil, Soňa Hermanová, Lukáš Richtera, Josef Jančář 

Brno University of Technology, Institute of Materials Science 


kroutil@fch.vutbr.cz 

The IR and Raman spectra of the lanthanide complexes of the general formula 

Cp* 2 LnCl 2 Li(OEt 2 ) 2 (where Ln = Sm, Nd) have been computed at HF and DFT 

(B3LYP) level using 6-31G(d) basis set for H, C, O and Li atoms, and large-core 

quasirelativistic Stuttgart/Dresden effective core potentials with appropriate basis 

sets for Ln and Cl. Theoretically predicted spectra have been confronted with experimentally 

observed ones and experimental IR and Raman bands were assigned 

to normal modes on the basis of HF and DFT calculations. All the predictions were 

done using Gaussian and GAMESS software packages. 

Keywords: neodymocene, ab-initio 

.


Posters 

6-P14 Comparison of Various Plasmachemical Processes 

with Respect to the Gas Temperature 

. 

L. Lazar, P. Synek, M. Karásková, M. Eliáš, O. Jašek, L. Kelar, V. Buršíková, 

P. Konupčík, L. Zajíčková 

Department of Physical Electronics, Faculty of Science, Masaryk University 

Kotlářská 2, 61137 Brno, Czech Republic 

lucasl@physics.muni.cz 

In this work optical emission spectroscopy (OES) is used for the determination 

of molecules rotational temperature in miscellaneous types of plasmachemical 

processes. This temperature approximates the temperature of neutral gas due 

to frequent collisions of the gas particles. A program for simulation and fitting of 

optical emission spectra of diatomic molecules was developed to obtain rotational 

temperature from the structure of rotational lines of given vibrational band. 

The spectra were measured in the microwave plasma torch at atmospheric 

pressure used for synthesis of carbon nanotubes with the catalyst either on the substrate 

or in the gas phase. The process is the high temperature deposition with correspondingly 

high rotational temperature of thousands of degrees. Another high 

temperature process, which was studied by OES, was the deposition of ultrananocrystalline 

diamond films in microwave bell jar (ASTeX type) reactor at the pressure 

of 7.5 kPa. In contrary, the deposition of amorphous diamond like carbon (DLC) 

films was carried out in the low pressure rf plasma in which the rotational temperature 

was close the 300 K. 

Keywords: optical emission spectroscopy, rotational temperature, plasma 

enhanced chemical vapor deposition


Posters 

6-P15 EPR Study of Nitroxide Radicals Generated 

from Some Secondary Alkyl-Aryl and Aryl-Aryl 

Amines 

Petr Majzlík, Ladislav Omelka, Jan Světlík, Peter Rapta 



majzlik@fch.vutbr.cz 

The series of secondary alkyl-aryl and aryl-aryl amines was subjected to the 

reaction with 3-chlorperbenzoic acid in different solvents. In the case of amines having 

two –NH– groups in the molecule two types of nitroxide radicals were registered, 

in dependence either on the structure of amine or the nature of solvent. The 

EPR spectra of generated radicals were interpreted using simulation method. The 

EPR parameters of generated radicals were determined. 

Keywords: amines, 3-chlorperbenzoic acid, EPR, nitroxide radicals 

.


Posters 

6-P16 Roughness of Coated Papers Treated in 

Atmospheric Plasma 

. 0 

Milan Mikula, Zuzana Pobudová, Adam Gilan 

FCHPT STU, 812 37 Bratislava 

milan.mikula@stuba.sk 

Coated papers were treated in standard dielectric barrier discharge (DBD) in 

air, nitrogen and added organic vapour mixture at atmospheric pressure. 

Modified papers were characterized by atomic force microscopy (AFM – topography 

and RMS roughness), water adsorption-desorption kinetics, contact angle 

measurements and absorption FTIR spectroscopy (ATR techniques). 

Roughness and other surface changes will be discussed in correlation with the 

applied treatment of the paper. 

Keywords: roughness, coated paper, plasma


Posters 

6-P17 Influence of Dye Structure on Its Decomposition 

by Electric Discharge in Water Solution 

Jana Pajurková, Zdenka Stará, František Krčma 



xcpajurkova@fch.vutbr.cz 

During last ten years, electrical discharges generated in water have been widely 

studied with a great focus on the application in water treatment. Following these 

worldwide trends, our experimental aparatus has been constructed for the investigation 

of electrical discharge effects on water solutions containing organic compounds, 

especially dyes. 

Experimental device consists of a batch discharge reactor, where the diaphragm 

discharge is created in a small pin-hole in the dielectric diaphragm dividing two 

electrode spaces, and of the high voltage source providing constant DC voltage up 

to 2 kV. Due to the application of DC voltage into the system, two kinds of plasma 

streamers are formed on each side of the diaphragm. These streamers differ especially 

in their energy dissipation and thus they substantially influence further chemical 

reactions, for example decomposition of the dye molecule. 

This paper presents results from the degradation of various organic dyes in 

the diaphragm discharge and compares obtained results from the viewpoint of dye 

chemical structure, electrode space and contens of electrolyte. First attempts show 

a significant dependence of decoloration efficiency on electrolyte kind as well as on 

the electrode polarity. Influence of dye structure is discussed, too. 

Keywords: electrical discharge in water, dye decomposition 

. 1


Posters 

6-P18 Influence of Mmmk Neutralization on Stability 

of Models of Historical Documents 

. 2 

M. Petrovičová, B. Havlínová, H. Paulusová 



miroslava.petrovicova@stuba.sk 

Archives, libraries, museums and galleries all over the world keep large numbers 

of manuscripts and drawings created by iron gall inks which suffered a continuous 

process of decay. The chemical nature of ink is very important from the 

conservation point of view and without its understanding it is not possible to solve 

the problems of paper support degradation. 

In the world several deacidification methods are used. These methods can be 

classified into water-based and non-aqueous processes. These processes make use of 

different combination of solvents, and they are suitable for different types of paper. 

This present study deals with application of neutralization reagent MMMK 

(Methoxy magnesium methyl carbonate), on documents containing iron-gall inks 

and its influence on document properties after stabilization. 

Keywords: deacidification, MMMK


Posters 

6-P19 Influence of CSC Booksaver Deacidification 

Process on Stability of Arylmetane Dyes on Paper 

M. Petrovičová, M. Ďurovič, E. Belányiová, B. Havlínová 



miroslava.petrovicova@stuba.sk 

Arylmethane dyes belong to the ones of the earlier synthetic dyes. At first the Fuchsine 

and Methyl Violet were prepared at the end of nineteenth century. Discovery of the reaction 

of phosgene with dimethyl aniline by Michler in 1867 was the breakthrough. Based on this 

reaction Caro and Kern prepared Crystal Violet in 1883. In 1877 the preparation of Malachite 

Green was published. Malachite Green is used in industrial scale up to the present time. 

Other arylmethane dyes based on hyroxytriphenylmethane and xanthene compounds were 

prepared letter. Latter, trihenylmethane dyes were by sulphonation of basic dyes. 

Low cost, color brilliance and substantiality, stability in solutions excelent writing 

properties was the reason of quick spread over the world. Low light fastness and chemical 

stability were considered as second-class factor. Today, these drawbacks come forth and lot 

of documents with inks based on these dyes requires immediate preservation action. 

The aim of this paper is study of influence of relative humidity and pH of paper support 

on complex properties of arylmethane inks. As a deacidification agent in our experiments, 

CSC Booksaver utilized in mass deacidification of acidic historical documents was used. 

Keywords: Booksaver, Arylmetane dyes 

.


Posters 

6-P20 Effect of Accelerated Ageing on Properties of 

Parchment 

. 

M. Petrovičová, B. Marušicová, M. Ohlidalová, B. Havlínová, 



marusicova@orangemail.sk 

Parchment is a specially treated form of leather that is soft and durable, making it an 

excellent writing material. High quality parchment is sometimes referred to as vellum. It is 

known that parchment was used as a writing material as early as the Ptolemaic era, and it was 

in fact preferred over papyrus in northern regions, where the climate can be unfavorable for 

papyrus, and also in Near Eastern regions. 

The aim of this task is regard of influence of accelerate ageing on parchment properties 

and comparison three different kinds of accelerate ageing together. 

Keywords: Parchment, accelerated ageing


Posters 

6-P21 Quality of Soil Humic Substances by SFS and UV- 

VIS Spectroscopy 

Ľubica Pospíšilová, Naděžda Fasurová 

Mendel University of Agriculture and Forestry, 

Institute of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, 

Zemědělská 1, 613 00 Brno, Czech Republic 

lpos@mendelu.cz 

In this paper we followed UV-VIS, SFS spectra and fractionation of different origin 

soil humic subctances (HS). We selected HS from following soil types – Modal 

Cambisol, Litic Cambisol, Modal Chernozem, Modal Rendzina and Modal Pararendzina 

(Czech soil samples). 

HS fractionation is contributed to the determination of humic acids 

(HA) and fulvic acids (FA) content. Ratio HA/FA is one of the important 

criteria for evaluation of soil quality and health. Results indicated 

that HA/FA ratio was decreasing together with HS quality as follows: 

Modal Chernozem < Modal Rendzina < Modal Pararendzina < Modal Cambisol 

< Litic Cambisol. Linear correlation between optical indexes (Q 4/6 ) and fractional 

composition of HS was found. Optical curves and indexes are able to indicate chemical 

properties and structure of HS. Highest absorbance in UV-VIS and SFS ranges 

was in Modal Chernozem. SFS spectra showed five main fluorophore peaks at: 359, 

450, 469, 489, 501 nm. All samples had similar fluorescence behaviour as Elliot soil 

humic acid standard (IHSS). Fluorescence of studied samples was compared with 

literature data and/or with standard. Samples contained substitued naphtalene and 

coumarine derivates. 

We came to the conclusion that samples origin was an important factor influencing 

HS content and quality. 

Keywords: HS fractionation, UV-VIS spectra, SFS spectra 

.


Posters 

6-P22 Sensoric Properties of Aromatic and 

Heterocyclic Compounds with Conjugated Bonds 

. 

Ota Salyk, Pavel Bednář, Oldřich Zmeškal, Jan Vyňuchal 



salyk@fch.vutbr,cz 

Compounds of the group of diketo-pyrrolo-pyrroles, perylenes and other semiconducting 

molecular organics with affinity to various gases, namely hydrogen, were 

synthesized and characterized. Their ability to serve as a sensing element material 

in gas sensors was evaluated. 3,6-di-pyridin-4-yl-2,5-dihydro-pyrrolo[3,4-c]pyrrole- 

1,4-dione (DPPP) was tested as a reference, than its derivative compounds and other 

relative compounds with potentially sensing group in molecules were considered. 

The molecular and crystallographic conformation in thin films prepared both from 

solution and vacuum deposition was focused. Proton absorption influence on absorption 

spectra variation and changes in conductivity in gap electrode arrangement 

were tested. The spatial position of active group in crystal, possibility of gas 

transport and capture at active position as well as easy gas release was considered 

and examined. Fabrication of the sensor and electrode material for achieving of high 

sensitivity and selectivity, rapid response and recovery of such sensing element is 

also considered. 

Keywords: sensor,organic semiconductor.


Posters 

6-P23 Structure of 1,4-diketo-3,6-bis-(4´-dipyridyl)pyrrolo-[3,4-c]pyrrole 

and Relative Compounds 

Ota Salyk, Vítězslav Frank, Jakub Stejkora, Jan Vyňuchal 



salyk@fch.vutbr,cz 

Nitrogen atom in the pyridyl ring reveals strong proton affinity and the rest 

of the molecule poses an electron transport. Convenient crystallographic structure 

creates a carrier transport path through the crystal. These circumstances can affect 

conductivity of the material via proton concentration and can serve as proton sensitive 

substance for proton sensors and by addition of catalytic hydrogen dissociation 

centres we can obtain hydrogen sensor. 

3,6-di-pyridin-4-yl-2,5-dihydro-pyrrolo[3,4-c]pyrrole-1,4-dione (DPPP) and 3phenyl-6-pyridin-4-yl-2,5-dihydro-pyrrolo[3,4-c]pyrrole-1,4-dione 

were prepared 

both in thin film and monocrystals and their crystallographic structure was investigated 

by x-ray difractometry. Program ORTEP was used for the construction of the 

crystallographic model. It was compared with the structure of 3,6-diphenylpyrrolo(3,4-c)pyrrole-1,4-dione) 

(DPP) as the reference for various derivatives. 

Keywords: hydrogen sensor, x-ray difractometry, 

.


Posters 

6-P24 The Influence of Mechanical Activation on the 

Production of the Humic Acids in the Brown 

Coal 

. 

M. Skybova, M. Lovas 



skybova@saske.sk 

One of the perspective trends of non fuel usage of coals is the production of 

humic preparations on its base, which are to be used in different branches of industry 

and agriculture. 

Activating grinding of brown coal in the equipments of intensive grinding raises 

significant surface structural changes of ultra-fine active powders that depend 

on the grinding intensity, i.e. grinding time and revolutions of a grinder. 

The paper deals with activation effect for preparation and transformation of 

humic acids structure in brown coal. The effect of activation grinding for yield, 

structure and granulometry of humic products was compared. It has been proved 

that with an optimum grinding time of 20 minutes and revolution amplitude of 400, 

there can be achieved a double increase of humic acids contents. In the process of 

activated coal grinding there occur insignificant SA changes due to the increased 

humic acids contents. 

The effect of mechanically activated humic acids was compared with products 

of mechanocemical leaching by methods GACL. The granulometrics analysis were 

carried out using apparatus Sympatec Helos, Rodos, Germany, in wet conditions. 

The structure of mechanically activated coal of isolated humic acids was performed 

by NMR and FTIR methods. 

Keywords: humic acids, coal


Posters 

6-P25 Numeric Modelling of V-T Process in Nitrogen 

Ground State under Post-Discharge Conditions 

Ivo Soural, František Krčma, Vasco Guerra 


Purkyňova 118, CZ-61200 Brno, Czech Republic 

xcsoural@fch.vutbr.cz 

The post-discharge kinetics is a very complex problem where many different 

processes, that are vibrationally specific, must be included. The radiation of nonradiative 

relaxation processes leads directly to the establishing of thermodynamic 

quilibrium. A special interest deals with the processes of metastables. In presented 

numeric model of nitrogen plasma, the vibration distribution function (VDF) was 

studied in time. About 90 % of total energy in plasma was dissipated into vibration 

levels and changed to the electron excitation, ionization, dissociation and another 

processes. Thus more processes such as v-t, v-v, etc. were necessary to know for the 

calculation of VDF in time. First step was the computer simulation of v-t process 

for the collision of N 2 molecule with N 2 (X, v). Subsequently, probabilities of the v-t 

transfer were calculated from the SSH theory at particular discharge conditions. 

Finally, the VDF model in time was calculated from the obtained transfer probabilities 

and initial concentrations in active discharge, however for the v-t process, only. 

Population vibration levels in VDF were changing to the Boltzman distribution. 

Keywords: numeric model of nitrogen plasma, vibration distribution function 

(VDF) 

.


Posters 

6-P26 Evaluation of Intake Cappability of LCM 

Surface by Adsorption Dyeding Method and its 

Utilization for Evaluation of Changes in Surface 

Polarity Caused by DCSBD Low-Energy Plasma. 

. 0 

Radovan Tiňo, Lívia Beňová,Svetozár Katuščák 

Institute of Polymer Materials, Department of Chem. Technology of Wood,Pulp and Paper, 


Radlinského 9, 812 37, Bratislava, Slovak Republic 

radovan.tino@stuba.sk 

Polarity transformation causes the change of water repellent and absorption 

properties of dyestuffs which can be used in quality control of wood surface hydrophobisation. 

Optical testing methods performing in the visible part of the spectra 

are non-destructive and have antropomorphous character. 

Polarity of lignocellulosic materials (LCM) is usualy evaluated by spectral 

methods as well as by measurement of water droplet contact angle and measurement 

of surface energy of solid surfaces. These methods are time and cost consuming 

whereupon new fast method for evaluation of changes in surface polarity of 

LCM by measurement of three chromatic components of modified and unmodified 

surface, after dyeding with choosen standard dye, in systeme CIE Lab has been developed 

and verified. The data of the 3 orthogonal and 2 cylindrical color parameters 

were measured and color diferences between control and DCSBD plasma modified 

wood surfaces were measured. Results show that change of wood surface polarity 

in most cases leads to change in ability to absorb color substances. By this method it 

is possible to achieve information about hydrofility and/or hydrophobicity of LCM 

surface. Correlation between classical methods of polarity evaluation and with the 

new method has been found. Coeffitien of correlation is R 2 = 0,8291. 

Keywords: DCSBD plasma, polarity, lignocellulosic, nondestructive evaluation


Posters 

6-P27 Evaluation of Effiency, Release and Oxidation 

Stability of Seabuckthorn Microencapsulated 

Oil using Fourier Transformed Infrared 

Spectroscopy 

Monica Trif and Carmen Socaciu 

Department of Chemistry and Biochemistry, 

University of Agricultural Sciences and Veterinary Medicine, 

Cluj-Napoca, 400372, Romania, 

monica_trif@hotmail.com 

This work examines microencapsulation effiency, the release and oxidation 

process of seabuckthorn oil encapsulated in ionotropically cross-linked alginatecarrageenan 

complex beads. 

The high content of polyunsaturated fatty acids, carotenoids and other oxygen 

sensitive lipid nutrients make this oil susceptible to oxidation, which limits its 

applications. 

The encapsulation efficiency of seabuckthorn oil was studied by UV-Vis spectroscopy 

and correlated with microscopical examination of emulsion stability. Seabuckthorn 

oil encapsulation in alginate-carrageenan complex beads was confirmed 

using Fourier transformed infrared (FTIR) spectroscopy. 

To monitor the stability of oil before and after encapsulation was done using 

FTIR spectroscopy. The oxidation process under UV light (254 μm) over time (after 

1h, 4h and 6h) was monitored calculating the ratios between absorbances bands 

of specific oxidation markers, of both free and encapsulated oil in alginate-carrageenan 

complex. 

The encapsulated oil showed a better stability against oxidation comparing 

with free oil monitored directly by FTIR, suggesting that this technique is suited to 

monitor the oxidation process. 

The release rates in methanol, hexane and tetrahydrofuran were measured. In 

hexane the release was slower than in the case of methanol and the best to release 

bioactive molecules was tetrahydrofuran. 

Keywords: seabuckthorn oil, encapsulation 

. 1


Posters 

6-P28 13C NMR Spectroscopy of Humic Acids Ectracted 

from Incubated Plant Residues in Haplic Luvisol 

. 2 

Anton Zaujec, Jozef Šima, Juraj Chlpík, Nora Szombathová, 

Erika Tobiašová 

Agricultural University of Nitra, Dept. of Pedology and Geology, 

Tr. A. Hlinku 2, 949 76 Nitra, Slovakia 

e-mail: Anton.Zaujec@uniag.sk 

Post harvest residues (maize, alfalfa, wheat and green manures) were incubated 

in soil (Haplic Luvisol) for long time and HA investigated. 

Humic acids were extracted from composted post harvest residues (corn, alfalfa, 

wheat and green manures) and their variants with Haplic Luvisol after 1 year duration 

time of decomposition. Transformation processes of these plant residues where 

studied under laboratory conditions. Humic acids from plant residues are not completly 

formed and undergo futher transformation. Plant residues changed structure 

HA´s extracted from incubated soil with studied post harvest residues. As humification 

proceeds the content of HA’s carbon slowly increases, while the content of 

hydrogen and H:C ratio decreases. This is evidence of progressing aromatization of 

the HA’s molecules. The carboxyl group content of HA’s also increased slowly. 

From the percentage distribution of the carbon atoms, as determined by 13C- 

NMR, the results show that composting gives rise to the formation of the HAs that 

are more aromatic and less aliphatic carbon. 

Keywords: Haplic Luvisol, plant residues, humic acid, elemental composition, 

13C-NMR, HA aromaticity

Section 7: 

Polymers and Polymer 

Composites 

.1

Lectures 

.

Section – Polymers & Polymers Composites 

Lectures 

7-L01 The Way of Stereoregularity Determination of 

Polypropylene 

Adam Hoza, Jan Kratochvíla, Soňa Hermanová 

Institute of Materials Science, Brno University of Technology 


xchoza@fch.vutbr.cz 

The purpose of this contribution is to evaluate and compare isotactic index determination 

of polypropylene homopolymer by extraction and solubility methods. 

A series of nine PP powder samples possessing molecular weights in the range from 

29×10 3 to 154×10 3 g mol –1 was investigated. Both the methods afforded fractionation 

of polypropylene into two portions, the soluble fraction and the insoluble one. 

Extractions were carried out in a modified Kumagawa extractor. Solvent extracts 

were obtained from polypropylene powders using several organic solvents 

(n-pentane, n-hexane, n-heptane, and n-octane) at their boiling points. 

Soluble fractions were prepared by dissolving polypropylene powder in high 

boiling solvents (xylene, decahydronaphthalene, and 1,2,3,4-tetrahydronaphthalene) 

and cooling polymer solutions to 23°C. 

All the fractions were characterized using 13 C-NMR, DSC, and SEC methods. 

Comparison of extraction and solubility methods showed a significant differences 

in the amount, structure, molecular weight, and crystalinity of soluble and insoluble 

fractions. 

Keywords: Isotactic index, polypropylene, extraction 

.


Lectures 

7-L02 The Crystallization Kinetics in Semicrystalline 

Nanocomposites 

. 

Kateřina Hynštová, Jan Kalfus, Josef Jančář 


Purkynova 118, 612 00 Brno, Czech Republic 

xchynstova@fch.vutbr.cz 

Nucleation and crystal growth greatly affect morphology and, thus, mechanical 

properties of semicrystalline polymers. Presented contribution brings an alternative 

approach to the description of crystallization kinetics in polyolefines filled 

with weakly interacting nano-fillers. It has been shown that the concept of chain 

reptation in a melt can be successfully implemented into the classical kinetic surface 

nucleation theory. In nanocomposites, high specific surface area is able to easily 

induce the chain immobilization even at very low filler loadings. In the vicinity of 

the filler surfaces, polymer chains exhibit retarded reptation motion due to the chain 

immobilization caused by the filler-polymer interaction. 

Keywords: nanocomposites, crystallization kinetics, reptation


Lectures 

7-L03 Effect of Hexamethyldisiloxane Solution of 

Zirconium Propoxide on Permanence of Paper 

Soňa Kirschnerová, Katarína Vizárová, Svetozár Katuščák 

Institute of Polymer Materials, Department of Chem. Technology of Wood, Pulp and Paper 

Slovak University of Technology, Radlinského 9, 831 07 Bratislava, Slovak Republic 

sona.kirschnerova@stuba.sk 

Double alkoxides, combination of chemical elements Al-K, Sn-Mg, Zr-Ca, used 

like neutralizing agent in a suitable solvents, such as alcohols, floro-chlorohydrocarbons, 

benzine hydrocarbons and siloxanes. Disadvantage upper alkoxides is formation 

of visible deposit. The aim of this work is to verify effect of hexamethyldisiloxane 

solution of zirconium propoxide on permanence of newsprint paper. 

Newsprint paper with extract pH (pH v ) 5.5–6.0; grammage (m s ) 45 gm –2 , containing 

55% of mechanical bleached groundwood, 20% of bleached kraft pulp, 15% 

scrap fibres and 10 % clay. Samples were aged in hermetically sealed bags at 98 ± 2°C 

during 0, 1, 2, 3 and 5 days. Changes of folding endurance (ω), breaking strength 

(l t ), concentration of carbonyls groups (∆c c=o ), pH v , total color difference (∆E) were 

studied. 

Utilization of studied modification system resulted in smooth surface of modified 

paper without wraps and formation of visible deposit on the surface. Artificial 

ageing causes decrease of l t in about of 15 % in comparison with the control paper. 

There was also observed decrease intensity of belt 1735 cm –1 (carbonyls groups) in 

comparison with control sample measured by FTIR. 

Keywords: zirconium, non polar solvent, newsprint, artificial ageing 

.


Lectures 

7-L04 Modification of Polypropylene Through Radical 

Grafting in Melt 

. 

František Kučera 

Brno University of Technology, Faculty of Chemistry, Institute of Materials Science 

Purkynova 118, CZ-61200 Brno 

kucera-f@fch.vutbr.cz 

Modification of polymeric material based on polyolefins, especially polypropylene 

with functional monomers is a certain way to obtain advanced materials 

with improved technological properties. Functionalization of polypropylene by 

grafting with polar monomer itaconic acid or itaconic anhydride using 2,5-dimethyl-2,5-bis(tert-butylperoxy)hexane 

(Luperox 101) as radical initiator carried out in a 

polypropylene melt in a static mixer or in Brabender extruder. The effect of reaction 

conditions on the yield of grafting was observed by varying reaction time, ratio of 

monomer to initiator concentration, temperature etc. It was found expressive influence 

of monomer/initiator ratio onto conversion of monomer and β-scission of polypropylene 

chain which cause increase in melt flow index. From the practical point o 

view seems technologically interesting method based on PP grafting in presence of 

fillers such as glass fibers, calcite, cellulose etc. for composites manufacturing. 

Functionalization of polyproplyene using styrene and dicumylperoxide or 2,5dimethyl-2,5-bis(tert-butylperoxy)hexane 

is a way to produce new slightly crosslinked 

polymeric materials or a way to compatibilization of polystyrene and polypropylene. 

Keywords: grafting, reactive extrusion


Lectures 

7-L05 Properties of Polypropylene Sheets Hot 

Compacted With Oriented Polypropylene Tapes 

Jaroslav Kučera, Jiří Sadílek, Blanka Rýznarová 

POLYMER INSTITUTE BRNO, spol. s r.o., 

Tkalcovská 36/2, 656 49 Brno, Czech Republic 

kucera@polymer.cz 

The properties of hot compacted polypropylene sheets containing interlayers 

of oriented polypropylene tapes have been examined with respect to the temperature 

of pressing. The tensile modulus, tensile strength and dart drop properties 

were measured. It is shown that only a narrow “temperature window” is suitable 

for compacting and that the properties of such polypropylene/polypropylene composite 

are improved in comparison with those found for sheets moulded or extruded 

from the same type of pure polypropylene. 

The result of compacting process is correlated with the properties of annealed 

single polypropylene tapes. The tensile properties and the structure parameters (in 

terms of DSC characteristic melting values) of these tapes were measured. 

.


Lectures 

7-06 Biodegradation of Modified Polyurethane Foams 

.10 

Obruča Stanislav, Márová Ivana, Ondruška Vladimír, 

Vojtová Lucy, David Jan 



e-mail: xcobruca@fch.vutbr.cz 

Increased production of non-degradable polymer waste by modern industrial 

society is accompanied by necessity to solve problems with negative effects to 

environment. In agreement with recent trends to avoid the accumulation of environmental 

pollution new modified polyurethane (PUR) foams were synthesized. 

Biopolymer components (acetyl-, hydroxyethyl- and carboxymethyl cellulose, 

acetylated starch, glutein) were used as modifying agents. In this work biodegradability 

of modified PUR foams was tested using bacterial single strain (Arthrobacter 

globiformis), mixed bacterial culture (Thermophillus sp.) and selected moulds strains 

(Fusarium solani, Alternaria alternata). In a complex comparative study standard biodegradability 

tests in laboratory conditions, in model composting conditions and 

also in different natural environments were used. In both bacterial systems, PUR 

biodegradation mechanism probably includes two additional effects: the abiotic 

decomposition followed by consequent utilization of degradation products by the 

bacterial culture. Under natural and composting conditions biodegradation of PUR 

foams was strongly influenced by abiotic factors. The highest degree of biodegradation 

was found in PUR modified by acetylated starch and carboxymethyl cellulose. 

In mould tests the presence of PUR led to enhanced biomass production. Significant 

effect of cell adsorption on PUR surface was observed too. In conclusion, tested 

microorganisms can be used for biodegradation of modified polyurethane foams. 

The degree of biodegradation is strongly dependent on the type of modifying agent, 

type of microorganism as well as on many abiotic factors. 

Keywords: biodegradation, polyurethane foams


Lectures 

7-L07 Reinforcing Mechanism in Layered Silicate 

Nanocomposites 

Lukáš Recman, Josef Jančář, Jan Kalfus 



jancar@fch.vutbr.cz 

During the last 15 years large volume of literature has been published on the 

reinforcement of various polymers with nano-sized particles. Among them, nanocomposites 

based on polymers reinforced with layered silicates were investigated 

most extensively. Despite the vast extent of experimental data published, there is 

still no unambiguous agreement on the true mechanism of reinforcement in these 

nanocomposites. In this contribution, experimental data on the reinforcement of linear 

amorphous and semicrystalline polymers with layered silicates collected from 

literature were analyzed using simple micromechanics model. It has been shown 

thath the primary reinforcing mechanism operating in amorphous nanocomposites 

below the matrix Tg and in semicrystalline nanocomposites below the matrix Tm 

is not related to the nano-scale processes and can be predicted using simple continuum 

mechanics model, satisfactorily. 

Keywords: layered structures, nanocomposites, mechanical properties, elastic 

properties 

.11


Lectures 

7-L08 The Influence of Mg Compound Paper 

Modification on Hornification and Strength 

Properties During Accelerated Ageing 

.12 

Štefan Šutý, Katarína Petriláková, Svetozár Katuščák, Soňa Kirschnerová, 

Michal Jablonský, Katarína Vizárová 

Department of Wood, Cellulose and Paper, FCHPT STU Bratislava 

Radlinského 9. 812 37 Bratislava, Slovakia 

stefan.sut@stuba.sk 

This paper is focused on influence of Mg compound modification on changes 

of WRV during accelerated ageing of paper. It has been recognized the two modification 

substances which have been different in concentration of Mg (as a MgO) 

and distribution in modified paper (on the surface and in volume of paper). The 

results show that both of them have influence on water retention value and hornification 

during accelerated ageing of paper. Mg substances have a positive influence 

on swelling of pulp fibres due to reactions with water in paper. The efficiency of 

modification substances is different. The more effective have been the substance 

with higher concentration of Mg. The results also showed the good correlation between 

WRV and strength properties of the paper. 

Keywords: hornification, water retention value, double falds, breaking length, 

accelerated ageing


Lectures 

7-L09 Impact of Processing on Degradation of 

Polypropylene Impact-Copolymer 

Jiří Tocháček, Soňa Hermanová, Josef Jančář, Jan Kalfus 

Polymer Institute Brno, Ltd., 

Tkalcovská 36/2, 656 49 Brno, Czech Republic 

The impact of processing on molecular structure and properties of polypropylene 

impact-copolymer (ICPP) were investigated. During processing realized in a 

laboratory scale by multiple extrusion on a 19 mm single-screw extruder, exposure 

to thermomechanical impacts caused a partial degradation of the ICPP polymer. 

Quantification of the extent of degradation and investigation of processes taking 

place in the homopolymeric and copolymeric phases of the material were the principle 

issues. 

It was found that most of the structural changes took place in a homopolymeric 

phase, which during processing mostly behaved as a standard PP homopolymer. Behaviour 

of this homopolymeric phase most influenced flow properties of the melt. 

The rubbery phase, on the other hand, consisting of ethylene-propylen rubber 

(EPR) and PE homopolymer, did not influence polymer melt so far. The changes 

due to processing were moderate and more than melt they influenced the impact 

characteristics of the solid matrix. Because the rubbery phase represents only a minority 

portion of the overall solid matrix (10-12 wt.%), identification of its structural 

changes is more complicated and cannot be realized by simple approaches. 

Attempts using a successive extraction of degraded material and its analyses, or detailed 

analyses of fracture-mechanical bahaviour of the solid phase provided some 

indirect indications on phenomena, which, as a consequence of processing, may 

take place in the heterogeneous rubbery phase of the ICPP matrix. 

Keywords: Degradation, polypropylene, impact-copolymer, processing, 

multiple extrusion, 

.1


Lectures 

7-L10 Polymer Semiconductors for Future Molecular 

Electronic Devices 

.1 

Martin Weiter, Martin Vala, Stanislav Nešpůrek 

Brno University of Technology,Faculty of Chemistry, 

Purkynova 118, 612 00 Brno, Czech republic 

weiter@fch.vutbr.cz 

Polymer semiconductors are poised as never before to transform the world of 

circuit and display technology. After more than 15 years of academic and industrial 

research worldwide the conjugated polymers has reached a very high level of outstanding 

material properties and the potential for different industrial applications 

is now emerging. Organic photovoltaic solar cells together with polymer photosensors 

and current switches represent one of the most promising applications which 

will be discussed. 

In spite of much progress that has been made in the understanding of the underlying 

science that controls the properties of the devices, still relatively little is 

known about the basic electron processes in these materials. Therefore the detailed 

studies of photonic processes, in particular those of photoinduced charge generation, 

transport and recombination, photoconductivity, electro- and photoluminescence 

and their dynamics were done a the results will be presented in particular 

with respect to their chemical structure of investigated materials. 

Keywords: polymer semiconductors, molecular electronic


Lectures 

7-L11 Molecular Modeling of Materials with Network 

Structure 

Jan Žídek, Josef Jančář 

Institute of Materials Science, Brno University of Technology, 


zidek@fch,vutbr,cz 

This paper deals with modeling of deformation properties of polymeric materials. 

Deformation properties are caused by two mechanisms: entropic and enthalpic 

contribution. In many materials one influence is prevailing and the second is negligible. 

However, there are materials with both comparable effects and composite 

model should be used. 

The entropic influence was calculated from network models. The rubbery material 

was considered 3D tetra-functional macromolecular network with entanglements 

acting as physical x-links. The deformation of the chains was calculated from 

single chain elasticity theory. 

The Enthalpic contribution was calculated from the function of energy density 

on strain tensor. Energy density was taken as the output of Molecular dynamics 

software.The complex model can be applied to whole spectra of network-like materials 

from gels to glasses. 

Acknowledgement: The research was supported by Ministry of Education under 

grant MSM 0021630501. 

Keywords: network, mechanical properties. 

.1

Posters 

.1


Posters 

7-P01 Physico-Chemical Properties of Functionalized 

Temperature-Sensitive Biocompatible Block 

Copolymers 

Jan David, Lucy Vojtová, Jiří Kučerík, Ludmila Mravcová, 

Martin Chytil, Miloslav Pekař, Milada Vávrová, Josef Jančář 

Institute of Materials Science, Faculty of Chemistry, Brno University of Technology 


xcdavid@fch.vutbr.cz 

Temperature-sensitive polymers based on hydrophobic poly(lactic acid-co-glycolic 

acid) (PLGA) copolymer and hydrophilic poly(ethylene glycol) (PEG) are being 

investigated extensively for medical applications because of their nontoxicity, 

biocompatibility and bioresorbability. 

In this work, the biodegradable A-B-A triblock copolymers made up from A 

blocks of PLGA and B block of PEG were synthesized via cationic ring opening 

bulk polymerization without solvent. The resulting copolymer was functionalized 

with itaconic acid anhydride in order to prepare macromonomer suitable for further 

cross-linking via double bonds and/or –COOH groups. The chemical structure of 

the copolymer was investigated by means of infrared spectroscopy and proton nuclear 

magnetic resonance. Physico-chemical properties were studied using differential 

scanning calorimetry, thermogravimetry and gel permeation chromatography. 

The sol-gel transitions and critical micellar concentrations of its water solutions. 

were evaluated via novel approach using rheometry and high resolution ultrasonic 

spectroscopy as well as the already known and frequently used test tube inverting 

method. 

This work was supported by the Ministry of Education, Youth and Physical 

Training of the Czech Republic under the research project MSM 0021630501. 

Keywords: biocompatible, macromonomers 

.1


Posters 

7-P02 Properties of Recycled Carbon Fibers 

.20 

Jan Grégr, Vladimír Kovačič, Miroslav Valeš, 

Bedřich Štekner, Karel Cihelník 

Department of Chemistry, Fakulty of Education, Technical university of Liberec 

KCH FP, TUL, Studentská 2,46717 Liberec 

jan.gregr@tul.cz 

Fibers in high performance composite materials are very expensive. For this 

reason is suitable to interesting about recycling waste products from fabrication of 

composites and composite parts after its lifetime. We made some experiments to 

obtain reinforcing carbon fibers from waste products and we evaluated properties 

of this recycled fibers. These fibers can be suitable for next exploitation. In contribution 

we demonstrate products of recycling and its properties. On the Department 

of Textile materials, Faculty of textile engineering and Department of chemistry, 

Faculty of education, Technical university of Liberec are developed testing methods 

for evaluating of recycled high performance fibers. We made analysis by the optical, 

gravimetric and SEM method; we realized tests of tensile strength, E-modulus, 

elongation to break and we studied wetting characteristics of these fibers. 

Keywords: recycling of composite, properties of recycled carbon fibers


Posters 

7-P03 Halosilyl-substituted Cyclopentadienyl 

Titanium Complexes as Catalysts for Styrene 

Syndiospecific Polymerisation 

Soňa Hermanová, Jan Merna, Milan Erben, Zdeněk Svačina 

Institute of Materials Science, Brno University of Technology 


hermanova-s@fch.vutbr.cz 

Syndiotactic polystyrene is an interesting material with a high melting point 

of 275 °C and a fast crystallization rate as compared with isotactic polymer. Its interesting 

properties which are similar to those of some more expensive engineering 

plastics are the reason for further scientific interest. In our previous work we reported 

the catalytic behaviour of novel half-sandwich titanium complexes; [TiCl 3 (η 5 - 

C 5 H 4 SiCl 3 )], [TiCl 3 (η 5 -C 5 H 4 SiMe 2 F)], [TiCl 3 (η 5 -C 5 H 4 SiMeF 2 )], [TiCl 3 (η 5 -C 5 H 4 SiF 3 )] and 

[TiF 3 (η5-C 5 H 4 SiMe 2 F)] in syndiotactic styrene polymerisation. The variation of halosilyl 

substitutent on cyclopentadienyl ligand affected both the catalytic activity 

and the properties of s-PS. 

In this contribution a series of polymerisation runs was carried out in a time 

scale of 20–120 min in order to compare the activity dependence of examined complexes 

on reaction time and thus to better evaluate their polymerisation behaviour. 

Results obtained are discussed with respect to the steric and electronic properties of 

ligand sphere influencing significantly the character of active species. 

Keywords: polystyrene, titanium complex 

.21


Posters 

7-P04 Synthesis of Modified Cucurbit [n]Urils 

.22 

Muhammad Shamsul Azim Khan, Vladimír Šindelář 

Department of Chemistry, Masaryk University, 

Kotlářská 2, 611 37 Brno, Czech Republic 


The supramolecular chemistry of cucurbit[n]urils (CB[n], n = 5-10), a synthetic 

receptor, have gained much attention in the new millennium for their exceptional 

molecular recognition ability. CB[n] is a macrocyclic compound self-assembled from 

an acid-catalyzed condensation reaction of glycoluril and formaldehyde or paraformaldehyde 

or mixture of aldehydes. Compared to the chemistry of cyclodextrins 

and calixarenes, however, that of CB[n] has developed slowly until recently, which 

may be attributed mainly to their poor solubility in common solvents and inability 

to functionalize these molecules. Our research is aimed to the synthesis and the 

characterization of new functionalized CB[n]. Recently, we have successfully synthesized 

new functionalized CB[n]s. Some of them are characterized and purified. 

As the final reaction mixture results in the composition of broad range mass distribution 

of different sized glycoluril oligomers, both substituted and unsubstituted 

cucurbit[n]urils (where n = 5-7), salts and other polymeric side reaction products, it 

has become a major concern to develop new methods for the purification. The purification 

steps were based on fractional precipitation of solids of various mass distribution 

using acetone or methanol and subsequent treatment of the collected solids 

or fractions depending on their solubility differences. In this review, methods of the 

separation and the characterization of the prepared CB[n] by MALDI-TOF-MS and 

NMR spectroscopy will be described. The new method of preparing functionalized 

cucurbit[n]urils propelled CB[n] chemistry to a new height as this partially not only 

solves the solubility problem but also opens up the gateway to the generation of 

tailor-made CB[n] derivatives. 

This research was supported by the grant 203/07/P382 from the Grant Agency 

of the Czech Republic. 

Keywords: cucurbit[n]uril


Posters 

7-P05 Application of Thermochromic Compounds in 

Polymeric Materials 

František Kučera, Petra Slánská 

Brno University of Technology, Faculty of Chemistry, Institute of Materials Science 

Purkynova 118, CZ-61200 Brno 

kucera-f@fch.vutbr.cz 

Temperature sensitive, color changing thermochromic material was prepared 

by compounding thermochromic materials with polypropylene matrix. A temperature 

stability and color stability were investigated in range of temperature 20– 

300 °C, temperature of color change was characterized. A method of thermochromic 

PP filament manufacturing was tested to produce three samples with different color. 

Properties of final fiber were investigated. 

Keywords: thermochromic 

.2


Posters 

7-P06 Polymer Photoelectronic Devices Based on 

Interaction Between π-Conjugated Polymer 

Matrices and Photochromic Molecules 

.2 

Jiří Navrátil, Martin Weiter, Martin Vala, Oldřich Zmeškal, Petr Toman, 

Stanislav Nešpůrek 


Purkynova 118, 612 00 Brno, Czech republic 

xcnavratil@fch.vutbr.cz 

The theoretical and experimental studies proved that the photochromic reaction 

in studied devices should eventuate in changes of optical and electrical properties 

of polymers such as luminescence and conductivity. The quantum chemical 

calculations showed that the presence of dipolar species in the vicinity of a polymer 

chain modifies the on-chain site energies and consequently increases the width of the 

distribution of hopping transport states. Optical switching was studied using standard 

absorption and photoluminescence spectroscopy. A strong photoluminescence 

quenching after the photochromic conversion caused by radiative energy transfer 

was observed. The influence of photoswitchable charge carrier traps on charge 

transport was evaluated by current-voltage measurement, Impedance spectroscopy 

and by photoconductive methods. It was shown that deep traps may significantly 

affect energy of the transport level, and thus control the transport of charge carriers. 

Thus, the photoelectronic device transforming optical signal to electrical one can be 

proposed. 

Keywords: optical sensor, organic semiconductors, charge transport, 

photochromic switching


Posters 

7-P07 Analysis of the Wood Surface Treated by Diffuse 

Coplanar Surface Barrier Discharge type 

Atmospheric Plasma 

Erika Nováková, Michal Letko, Radovan Tiňo, Ján Marták, Lívia Beňová 

Department of Chemical Technology of Wood, Pulp and Paper, Faculty of Chemical and Food 

Technology, Slovak University of Technology 

Radlinského 9, 812 37, Bratislava, Slovakia 

erika.novakova@stuba.sk 

Utility properties of polymer materials including wooden based materials are 

often determined by surface properties. Adhesion of coatings utilized to cover wood 

surface can be influenced by transformation of wood surface pH. 

Wood surface was treated by atmospheric plasma, which was generated by 

diffuse coplanar surface barrier discharge (DCSBD). It was discovered that after the 

plasma treatment, wood surface pH changed in relation to distance of wood surface 

from source of plasma (electrode). From the surface of Fagus silvatica, Quercus robur 

and Picea excelsa treated by the plasma, were obtained a cold water extracts. 

Total change of acid components concentration was determined by the acidbase 

titration. The highest increase of acid components up to 95 % was achieved 

from oak wood, which surface was in direct contact with plasma electrode. Subsequently 

there was determined change of concentration and presence of carboxylic 

acids by isotachophoresis. Concentrations of formic acid, acetic acid, propionic acid 

and butyric acid altered in relation to species of wood. The highest increse of formic 

acid up to 56 % was achieved in oak wood. 

Keywords: atmospheric plasma, isotachophoresis, wood surface, 

.2


Posters 

7-P08 Strategies for Enhancing 

Poly(3-hydroxybutyrate) Production in Selected 

Bacterial Strains 

.2 

Stanislav Obruča, Soňa Melušová, Ivana Márová, Zdeněk Svoboda 



E-mail: xcobruca@fch.vutbr.cz 

Polyhydroxyalkanoates (PHAs) are a group of bacterial polyesters that have 

received much interest in recent times due to their biodegradable nature and mechanical 

properties which are very similar to traditional plastics from petrochemical 

routes. The most common PHA is poly(3-hydroxybutyrate) (PHB). Many bacterial 

strains use PHB as carbon and reducing power storage material. 

Bacterial strains usually accumulate PHB during exposition to stress condition. 

Typical strategy, which is widely used for enhancing of production, is limitation 

with nitrogen, phosphor or another element when excess amount of carbon source 

is present. 

The influences of selected stress factors on accumulation of PHB were tested 

in this work. Limitations with nitrogen and/or phosphor source were tested during 

growth of bacteria on synthetic as well as natural waste carbon source. Oxygen 

limitation was studied too. Finally, peroxide stress, osmotic stress, ethanol stress 

and UV radiation were studied as additional stress factors to nutrition starvation. 

Combined effect of exogenous stress could lead to increased accumulation of PHB 

in selected bacterial strains. 

Keywords: poly(3-hydroxybutyrate), stress condition


Posters 

7-P09 Comparison of Biodegradability of Modified 

Polyuretne Foams and Polyurethane Elastomeric 

Films 

Stanislav Obruča, Ivana Márová, Jana Piechová, Lucy Vojtová, 

Marek Novotný, Jan David 



e-mail: xcobruca@fch.vutbr.cz 

Biodegradation of polymeric materials is important process which affects a 

wide range of industries. Polymer biodegradability depends on many factors as 

molecular weight, crystallinity or physical forms. Biodegradability of several modified 

polyurethanes (PUR) foams and films was tested using mixed thermophillic 

bacterial culture Thermophillus sp. Films and foams were modified by biopolymer 

component (10 % of acetyl-, hydroxyethyl- and carboxymethyl cellulose, 10 % activated 

starch). Both films and foams supported the growth of culture in comparison 

with culture without PUR. Unusual long lasting lag-phase was observed when 

culture was cultivated in presence of PUR films. This effect was probably caused 

by formation of some toxic components which inhibited the start of growth. Mass 

decreases of foams and films were very similar, but mechanism of biodegradation 

is probably different. Mainly abiotic factors are involved in biodegradation of PUR 

foams. Products of abiotic degradation are consequently utilized by bacterial culture. 

Abiotic factors play important role during biodegradation of PUR films too, 

but, surprisingly, films which are expected to be more rigid, were more susceptible 

to biotic degradation. Measure of biotic degradation positively corresponded with 

the growth of culture. This is probably caused by different incorporation of modifying 

agents into structure of film in comparison with foams. 

Keywords: biodegradation, polyurethane, foam, film 

.2


Posters 

7-P10 synthesIs of substItuted PolysIlylenes used as 

.2 

semIconductIve Polymers 

Imad Ouzzane, Soňa Hermanová, Martin Weiter, Stanislav Nešpůrek 



ouzzane@fch.vutbr.cz 

Polysilylenes are of considerable research interest because of their electronic, 

photoelectrical and nonlinear optical properties, and the effect of σ-electron delocalization 

along the chain. 

Polysilylenes are most often obtained by Wurtz reductive coupling of organosilanes 

with sodium metal in refluxing toluene. Therefore the metal is easily distributed 

throughout the solution. Unfortunately, the yields of polymers and the reproducibility 

of the reaction are usually quite poor. The contribution reports on new 

methods and procedure based on new operatory conditions that allowed us to gain 

in yield and quality of product obtained. 

The other aim of our work is to synthesize new model of polysilylenes attached 

directly to functional chromophores throughout a Suzuki coupling and investigate 

their electrical and optical properties. The proposed structure should be capable of 

light driven switching between high conductive and low conductive regime, and 

therefore to be used as current switch or actuator. 

Keywords: polysilylene, synthesis, organic semiconductors


Posters 

7-P11 Automation of PE-CVD Process for Preparing of 

Nanostructured Films 

Radek Přikryl 


Purkyňova 464/118, 612 00, Brno, Czech Republic 

prikryl@fch.vutbr.cz 

Plasma Enhanced Chemical Vapor Deposition (PE-CVD) is a power tool for 

designing of nanostructured films. The used technology is relatively complicated 

and condition-intensive in term of reproducibility of resulting material. The deposition 

system has many input parameters have to be set or continuously regulated. 

A controlling of the process parameters using automation technique can increase 

reproducibility of experiments. Automated measured data recording enable additional 

check of deposition process. The aim of presented work is a design and development 

of programmable automation system for deposition process parameters 

controlling. 

Keywords: nanostructure, PE-CVD, automation, plasmachemistry 

.2


Posters 

7-P12 Crystal Structure of an Lanthanide Sandwich 

Complex (C5Me5)2NdCl2Li(OEt2)2 

. 0 

Lukáš Richtera, Soňa Hermanová, Vojtěch Jančík, Jaroslav Cihlář 



richtera@fch.vutbr.cz 

The potential of lanthanide sandwich complexes of general formula 

(C 5 Me 5 ) 2 LnCl 2 Li(OEt 2 ) 2 in polymerisation catalysis is nowadays well established. 

The reaction mechanism of ethylene oligomerisation was published few years ago 

but there was no reliable proof of (C 5 Me 5 ) 2 NdCl 2 Li(OEt 2 ) 2 structure even though its 

synthesis was described already in 1981. 

The structure of (C 5 Me 5 ) 2 NdCl 2 Li(OEt 2 ) 2 was first proposed based on the similarity 

with the spectral data of compounds (C 5 Me 5 ) 2 LnCl 2 Li(OEt 2 ) 2 (Ln = Ce, Sm) 

whose structures were known as well as for some other lanthanide sandwich complexes 

(Pr, Nd, Gd, Dy, Yb and Lu) with different organic donors (e.g. THF or TM- 

EDA) and various substituents on the cyclopentadienyl rings. 

Although many structures of lanthanidocenes with the general formula Cp 2 Ln- 

Cl 2 MD 2 (Cp = cyclopentadiene or its derivative, Ln = lanthanide, M = alkali metal, 

D = organic donor) are known, others have been reported to have a quite unconventional 

structures and/or coordinations numbers. Therefore we have focused on the 

determination of the crystal structure of the title species a) to confirm or negate the 

structure predicted in already published studies; b) to get the missing experimental 

data for quantum mechanical study of this compound. 

The molecule of (C 5 Me 5 ) 2 LnCl 2 Li(OEt 2 ) 2 is localized on the mm symmetry site, 

which together with a heavy disorder of all organic residues made the structure determination 

rather difficult. However, it was possible to obtain data of satisfactory 

quality and finally confirm the molecular structure of this species. 

Keywords: lanthanidocene, structure


Posters 

7-P13 Computing Effective Properties of Composites 

Via FEM Simulations 

Miroslav Rolník, Jan Žídek, Josef Jančář 

Vysoké učení technické v Brně, Fakulta chemická, 


xcrolnik@fch.vutbr.cz 

A dependence of modulus of heterogeneous material on its composition is presented. 

A mathematical homogenization method was used for calculation this modulus. 

Input properties were portions of components, mechanical properties of these 

components and space conformation. Inclusions are supposed to be spherical and 

cylindrical, randomly distributed in space and with random parameters of generated 

shapes. The method for generating such geometry is presented. This generator 

was used to produce unit cell (representative volume element) and consequently 

effective properties were determined by mathematical theory of homogenization. 

Method was supposed for periodic material and for this assumption it give us formulas 

for these effective properties. 

Keywords: macroscopic material properties, heterogeneous material, FEM 

simulation 

. 1


Posters 

7-P14 Effect of Thermal History on 

Structure-Property Relationship in Oriented 

Polypropylene Films and Tapes 

. 2 

Jiří Sadílek, Jaroslav Kučera 


Purkyňova 118, 612 00 Brno, Czech Republic Czech Republic 

xcsadilek@fch.vutbr.cz 

Effect of temperature ageing on structure-property relationship in oriented 

polypropylene films and tapes is described in this contribution. Commercial grade 

polypropylene Mosten TB 002 (2.73 g/10min) was used as primary material. This 

material was extruded and film with 400 μm thickness was prepared by chill roll 

technique from polypropylene melt. Primary film was drawn with drawing ratio 5:1 

in the first step, then the film was cut to tapes and they were drawn on the hot plate 

with drawing ratio from 5:1 up to 11:1 in the second step. 

Free and fixed strips of films and tapes were aged at different temperatures. 

Unannealed and annealed samples were tested. Strength and elongation at break 

(tensile properties), degree of crystallinity and structure characteristics (DSC), stiffness 

by temperature scan and isothermal creep at DMA measurements, chain orientation 

(RTG) and shrinkage of free annealed film strips and tapes were measured. 

Keywords: drawing ration, creep, crystalinity, orientation


Posters 

7-P15 Bioresorbable Collagen-Hyaluronic Acid 

Scaffolds Cross-Linked Using a Water-Soluble 

Carbodiimide 

Alexandra Sloviková, Lucy Vojtová, Josef Jančář 

Institute of Materials Chemistry School of Chemistry Brno University of Technology 


xcslovikova@fch.vutbr.cz 

Porous collagen scaffold matrices containing hyaluronic acid (HA) are promising 

materials for tissue regeneration. In this work, two types of matrices were prepared 

(Coll-HA-1 and Coll-HA-2) followed by comparing their physical properties. 

The Coll-HA-1 was made by combination of freeze drying and air drying method 

while the Coll-HA-2 was only freeze dried, which is commonly used method. Prepared 

scaffolds were subsequently cross-linked using EDC/NHS system for different 

period of time. The amount of free amino-groups representing the cross-linking 

efficiency were determined using 2,4,6-trinitrobenzene sulphonic acid. 

Scanning electron microscope images of collagen-HA matrices demonstrated 

the difference in the scaffold 3-D structures. Samples of Coll-HA-1 were found to 

be smooth in comparison with porous Coll-HA-2. The hydrolytic stability at physiological 

conditions was longer then seven days for all samples. The bioinduction 

tests showed the increasing migration of mesenchymal cells in material contain HA 

in comparison with pure collagen. 

Acknowledgement: This work was supported by the Ministry of Education, Youth 

and Physical Training of the Czech Republic under the research project MSM 

0021630501. 

Keywords: collagen, hyaluronic acid, haemostatic 

.


Posters 

7-P16 Water Retention Value During Thermally 

Accelerated Ageing of Paper. 

. 

Štefan Šutý, Katarína Petriláková, Svetozár Katuščák, Michal Jablonský, 

Katarína Vizárová., Soňa Kirschnerová 

Department of Wood, Cellulose and Paper, FCHPT STU Bratislava 

Radlinského 9. 812 37 Bratislava, Slovakia 

stefan.sut@stuba.sk 

Water retention value (WRV) as a value for determination of swelling of the 

pulp fibres has been followed during thermally accelerated ageing of acid wood 

paper with 20 % content of chemical pulp fibres. The water retention value is significantly 

decreasing with increasing time of accelerated ageing. The relationships 

between WRV and strength properties have been investigated too. The evaluation of 

samples with mercury porosimetry have been find out that the cumulative volume 

of pores increasing with time of paper ageing and pore radius average and specific 

surface of pores decreasing. The results from mercury porosimetry support the 

hornification process during accelerated ageing of paper. It can be concluded that 

during thermal accelerated ageing the hornification is one of the reason of embitterment 

the paper. 

Keywords: hornification, water retention value, double falds, breaking length, 

accelerated ageing


Posters 

7-P17 Spin Probe Dynamics in Relation to Free Volume 

and Relaxation Dynamics of Poly(isobutylene) 

Helena Švajdlenková, Josef Bartoš 

Polymer institute of the Slovak Academy of Sciences 

Dúbravská cesta 9, 84236 Bratislava, Slovak Republic 

Upolhela@savba.sk 

We present the dynamics of a series of spin probes of different volume 

and shape in amorphous polymer poly(isobutylene) (PIB) as investigated by means 

of electron spin resonance (ESR) technique in relation to the free volume of PIB 

from positron annihilation lifetime spectroscopy (PALS) and the dynamic data from 

broadband dielectric spectroscopy (BDS) and nuclear magnetic resonance (NMR) 

spectroscopy. For the smallest spin probe TEMPO, the analyses of the spectral pa- 

rameter, 2 Z ' A , and the correlation time, c 

τ , vs. T plots provided the characteristic 

Azz 

ESR temperatures ( TX 2 τ τ 

1 , T50 G ) and ( T X1 

, T c ) which are consistent with the char- 

acteristic PALS temperatures 

PALS 

T g , 

L 

b 

T 1 = σ T b1 

, as obtained from the temperature 

dependences of the o-Ps lifetime, τ 3 , and the width of o-Ps lifetime distribution, 3 σ 

. In addition, the relationships between the spin probe size, W V Tempo , and the free vol- 

n h V g at the characteristic ESR temperatures indicate 

the influence of the free volume fluctuation. 

Finally, the mutual PALS and ESR findings are related to free volume fluctuation 

associated with secondary δ - or β - process, respectively. 

ume hole size distributions ( ) 

Keywords: poly(isobutylene); spin probe, free volume; dynamics 

.


Posters 

7-P18 Toughness Versus Strength and Stiffness of 

Polypropylene Filled with Submicron CaCO 3 

Particles 

. 

Petr Veselý, Eva Nezbedová, Bohumil Vlach 

Faculty of Mechanical Engineering, Brno University of Technology 

Technicka 2, 616 69 Brno 

wesa@email.cz 

Commercial available polypropylene (PPH) was modified with β nucleation 

agent to achieve enhancement of its toughness. The difference size of CaCO 3 particles 

supplied from Solvay Company was used as fillers. The influence of the size 

and mass content of the particles on the mechanical properties of composites were 

investigated. Particularly, the stiffness, strength and toughness were chosen as relevant 

parameters. The stiffness and strength were estimate by the means of tensile 

test while for toughness the J-R curve procedure was utilised. The results were correlated 

with microscopic observation. 

Keywords: PP/ CaCO 3 composites, mechanical properties.


Posters 

7-P19 Polysaccharides Modified Elastomeric 

Polyurethane 

Lucy Vojtová, Marek Novotný, Stanislav Obruča, Jana Piechová, 

Ivana Márová, Josef Jančář 

Institute of Materials Science, Faculty of Chemistry, Brno University of Technology 


vojtova@fch.vutbr.cz 

Increasing the degradability of biologically hardly degradable but very often 

commercially used polyurethane is being extensively studied in order to avoid environmental 

pollution. 

Proposed work deals with the synthesis of elastomeric polyurethane with various 

biodegradable fillers based on natural polysaccharides like carboxymethylcellulose 

sodium salt, 2-hydroxyethylcellulose, cellulose acetate and acetylated starch. 

Prepared samples were tested in term of mechanical and biodegradable properties. 

The morphology of samples was analyzed by scanning electron microscopy, chemical 

composition by infrared spectroscopy and the thermal stability was tested by 

thermogravimetric analysis. Applied methods proved that prepared samples have 

character of particle reinforced composite materials. Results showed that the best 

influence on mechanical properties have acetylated starch and gluten, which also 

demonstrated the highest thermal stability of modified elastomeric polyurethane 

samples. 

In a pilot study, biodegradation of modified polyurethane elastomers by mixed 

culture of thermophillic bacteria exhibited unusual growth characteristics strongly 

dependent on the type of biopolymer filler. 

Acknowledgement: This work was supported by the Ministry of Education, 

Youth and Physical Training of the Czech Republic under the research project MSM 

0021630501. 

Keywords: Polyurethane, Elastomers, Biodegradation 

.


Posters 

7-P20 Models of Organized Supermolecular 

Structures: Application to Collagen Fibrils and 

Dendrimers 

. 

Jan Žídek, Jolana Ščudlová, Josef Jančář 

Institute of Materials Science, Brno University of Technology, 

Purkynova 118, 61200 Brno, Czech Republic. 

zidek@fch.vutbr.cz 

This contribution deals with the research on organized supermolecular structures. 

These structures are present in many polymeric materials. Mainly in bio-materials, 

they cause biological activity. 

Collagen fibril and dendrimers are suitable materials for molecular modeling. 

Collagen is natural protein with long, fibrous structure. Its fibrils show high elasticity. 

This property makes the material useful for certain applications. Dendrimers are 

synthetic macromolecules with regular and highly branched architecture. Modeling 

of their properties can be useful for improvement of various molecular models. 

The molecular structure of collagen fibril and dendrimers was studied by molecular 

dynamics simulations. The structures were built in the GROMACS software. 

Elastic deformation of collagen fibril was investigated. It was found, that the fibril 

would return spontaneously to the undeformed state. Three reasons of elasticity 

were analyzed: 1. triple helix structure, 2. intermolecular interaction, and 3. special 

geometry of side chain groups. In case of dendrimers, shape and gyration radii were 

calculated from molecular models. Properties of these two materials are well described 

in literature. Therefore, modeling results can be compared to experimentally 

measured properties. 

Acknowledgement: The research was supported by Ministry of Education under 

grant MSM 0021630501.

Appendices 

I.1

I.2

Index 

A 

Abaee, M. S. 4.13, 4.22, 4.23 

Abbasi, H. 4.22 

Abrudean, M. 3.46 

Achimovičová, M. 3.19 

Adamechová, Z. 2.7 

Ahmadi, S. H. 1.21 

Almásiová, B. 1.22 

Altangerel, B. 2.21, 2.22 

Amberg-Schwab, S. 5.33 

Amblès, A. 6.7 

Andreeva. E. 1.23 

Andrei, S. 2.23, 2.99 

Angelovič, M. 1.24 

Angelovičová, M. 1.24 

Asl, J. D. 1.21 

Avdonin, P. 1.8 

B 

Babák, L. 2.24, 2.25, 2.127 

Bajčan, D. 1.109 

Bajziková, M. 3.7 

Bakajová, B. 6.25 

Bakalár, T. 1.102 

Baláž, P. 3.45 

Bálintová, M. 1.25, 1.51 

Baráček, J. 3.6 

Barna, D. 2.102 

Barošová. M. 2.87 

Bartackova, V. 2.26 

Bartáková, K. 2.27 

Bartoníčková, E. 3.20 

Bartoš, J. 40, 7.35 

Batelková, P. 2.27 

Bednář, P. 4.14, 6.46 

Bednáriková, A. 2.37, 2.38 

Běláková, S. 2.29, 2.30, 2.31 

Belányiová, E. 6.43 

Author Index 

Bele, C. 1.80, 2.28, 2.42, 2.89, 2.99, 

2.110, 2.120 

Bella, M. 1.110 

Benabbou, K. 5.10 

Beňová, L. 6.50, 7.25 

Bercea, V. 1.74 

Berns, A. E. 4.5 

Biris, A. B. 2.28 

Biris, A. R. 1.80 

Bjelkova, M. 2.76 

Bláha, L. 1.5 

Blahová, E. 1.100 

Bobáľová, J. 2.35, 2.81, 2.82, 2.90, 4.17, 

4.18 

Boček, P. 1.59 

Bogovič Matijašić, B. P.3 

Borkovcová, I. 2.27 

Bošiak, M. 2.52 

Bowie, A. 1.15 

Brandštetr, J. 3.5 

Brehuv, J. 1.11, 1.26, 1.35, 1.86, 1.87 

Breierová, E. 2.5, 2.9, 2.32, 2.39, 2.55 

Bretšnajdrová, E. 6.26 

Brezová, V. 1.110, 2.15, 5.5, 5.8 

Broscăţean, Ş. 1.91 

Brulík, L. 1.27 

Búgel, M. 1.102 

Bui, T.-H. 5.10 

Bukovský, V. 3.7 

Buňková, R. 2.100 

Burauel, P. 4.5 

Burdychová, R. 2.25, 2.33, 2.34 

Bursáková, P. 6.16, 6.27 

Buršíková, V. 6.38 

Bušinová, P. 6.28 

Butler. E. 1.15 

Buzinkay, A. 5.7 

Bžatek, T. 6.21 

I.

Author Index 

C 

Cabálková, J. 2.35 

Champion, E. 3.48 

Chereches-Panta, P. 4.16 

Chlpík, J. 1.85, 6.52 

Chomoucká, J. 1.103, 1.104, 5.6, 5.9, 

5.22, 5.28 

Chytil, M. 6.29, 7.19 

Ciesarová, Z. 2.6, 2.37, 2.38 

Ciglanská, M. 5.12 

Cihelník, K. 7.20 

Cihlář, J. 3.20, 7.30 

Cihlář, Z. 6.27 

Clemensová, G. 1.29 

Coloşi, T. 3.46 

Conte, P. 4.5 

Contreras-Acuña, M. 2.54 

Cozar, O. 2.63, 2.64 

Culea, E. 4.15, 4.16 

Culea, M. 2.62, 2.63, 2.64, 4.6, 4.15, 

4.16 

Cvačka, J. 1.116 

Cvengroš, J. 2.11 

Cvetković, D.D. 2.101, 2.102, 2.103 

Č 

Čajka, T. 1.16, 2.36 

Čáp, T. 1.5 

Čarnecká, M. 2.9, 2.39, 2.55 

Čáslavský, J. 1.6, 1.17, 1.28, 1.32, 1.73, 

1.100 

Častulík, P. P.6, 1.63 

Čejková, A. 2.59, 2.79, 2.109 

Čelechovská, O. 2.126 

Čelko, L. 3.21, 3.29 

Čeppan, M. 5.7, 5.12, 5.17, 5.27, 5.31, 

5.32, 5.37 

Černá, M. 5.29 

Černochová, L. 1.30 

Čertík, M. 2.7, 2.9, 2.56, 2.67 

Čéry, J. 1.94 

I. 

Čmelík, R. 4.17 

Čtvrtníčková, A. 6.5, 6.30 

Čuvanová, S. 1.37, 1.115, 4.7 

D 

Damia, Ch. 3.48 

Dankova, M. 2.129 

David, J. 7.10, 7.19, 7.27 

Debska, B. 1.85 

Derco, J. 1.22, 1.30 

Dhingra, O. D. 2.68 

Dičáková, Z. 2.41 

Dítě, P. 2.100 

Diviš, P. 1.27, 1.31 

Dobrylko, I. 1.8 

Dočekal, B. 1.39, 1.68 

Dočekalová, H. 1.27, 1.39, 1.68 

Dohnal, V. 2.25, 2.33 

Doležal, P. 3.29 

Doležalová Weissmannová, H. 1.32, 

1.33 

Dóriová, J. 5.27 

Doucek, A. 3.11 

Drábová, L. 2.40 

Dragoş, N. 1.74 

Drastík, M. 6.5 

Drbohlavová, J. 5.16, 5.22, 5.28, 5.30 

Drobná, B. 2.16 

Dudriková, E. 2.41, 2.45 

Dulf, E. 3.46 

Dulf, E.-H. 6.6 

Dulf, F. 2.28, 2.99, 2.120, 6.6 

Dulf, F. V. 2.42 

Dulp, F. V. 3.14 

Dumitrita, R. 2.43 

Duroňová, K. 2.44, 2.86, 2.87 

Dutková, E. 3.19 

Dvonka, V. 5.31, 5.32, 5.37 

Dvořák, R. 1.46 

Dvořáková, J. 1.7, 2.44 

Dvořáková, T. 2.39

Dvoranová, D. 5.5, 5.8 

Dyčka, F. 4.18 

Dzik, P. 1.103, 5.6, 5.9, 5.18, 5.22, 5.24, 

5.28, 5.29, 5.38, 5.40, 6.31 

Ď 

Ďurovič, M. 6.43 

E 

Ehrenbergerová, J. 2.71 

Ehrenbergerová, V. 2.86 

Eliáš, M. 6.38 

Endstrasserová, J. 2.125 

Erben, M. 7.21 

Ermolaeva, E. 1.8 

Eštoková, A. 1.34, 1.89, 3.24 

F 

Faragó, J. 2.124 

Faragová, N. 2.124 

Fári, M. 2.124 

Farkaš, V. 2.48 

Fasurová, N. 6.32 

Fatrcová-Šrámková, K. 2.45, 2.69 

Fedorová, E. 1.11, 1.26, 1.35, 1.86, 

1.87 

Felšöciová, S. 2.69 

Festila, C. 6.6 

Feszterová, M. 1.9, 1.36, 1.112 

Fiala, M. 3.36 

Fikselová, M. 2.69, 2.85 

Filka, P. 1.108 

Fiľková, I. 3.23 

Fišera, M. 2.126 

Fišerová, L. 1.67 

Flodrová, D. 2.47, 2.48 

Fortunová, Ľ. 1.37 

Frančáková, H. 2.85 

Francu, E. 1.38 

Francu, J. 1.38 

Frank, V. 3.13, 3.26, 3.43, 6.47 

Freirea, M. M. 2.68 

Furdíková, K. 6.11 

G 

Author Index 

Gábelová, A. 1.116 

Gaberová, L. 1.37 

Gago, A. 2.93 

Gallios, G. P. 1.88 

Garajová, S. 2.47, 2.48 

García-Barrera, T. 1.71, 1.72, 2.49, 

2.50, 2.51, 2.53, 2.54, 2.93 

Gebauer, P. 1.59 

Gedeon, O. 3.48 

Gemzicka, E. 1.111 

Gešperová, D. 3.8 

Gilan, A. 6.40 

Glashkina, L. 1.8 

Golian, J. 2.52, 2.114 

Goliáš, J. 2.44 

Gómez-Ariza, J. L. 1.10, 1.71, 1.72, 

2.49, 2.50, 2.51, 2.53, 2.54, 2.93 

Gómez de las Heras, E. 2.53 

Goncharov, N. 1.8, 1.79 

Gónzalez-Fernández, M. 2.50 

Grabner, G. 5.13 

Grasset, l. 6.7 

Grégr, J. 7.20 

Gregušová, M. 1.39 

Guillard, C. 5.6, 5.10, 5.11, 5.16, 5.36 

H 

Haddad, P. 1.15 

Hajšlová, J. P.7, 1.16, 1.40, 1.41, 1.54, 

1.76, 2.8, 2.14, 2.17, 2.26, 2.36, 

2.40, 2.58, 2.71, 2.75, 2.76, 2.78, 

2.106, 2.122 

Hakenová, M. 1.40 

Halasová, T. 6.13 

I.

Author Index 

Halienová, A. 2.9, 2.39, 2.44, 2.55, 

2.88 

Hančuľák, J. 1.11, 1.26, 1.35, 1.86, 

1.87 

Hanus, J. 3.7 

Hanusová, V. 2.9 

Harazim, J. 1.82, 2.77 

Harbuľáková, V. 3.24 

Hároniková, A. 2.39 

Has, I. 2.89 

Havlica, J. 3.5, 3.6, 3.12, 3.31, 3.32, 

3.33, 3.34, 3.35, 3.36, 3.37, 3.41, 

3.42, 3.43 

Havlínová, B. 5.12, 5.31, 5.32, 5.34, 

6.42, 6.43, 6.44 

Havrlentová, M. 2.56 

Ház, A. 2.91, 2.92 

Hellingerová, L. 1.114 

Hermanová, S. 6.37, 7.5, 7.13, 7.21, 

7.28, 7.30 

Hermanová. M. 3.26 

Herrmann, J.-M. 5.11, 5.16, 5.36 

Herzogová, J. 2.115 

Hiller, E. 1.105, 1.113 

Hilscherová, K. 1.5 

Hlaváčková, I. 1.17, 1.73 

Hoferkova, P. 2.33 

Hohnová, B. 2.57 

Hojerová, J. 2.91, 2.113 

Holoubek, I. 1.5 

Holubcová, R. 6.14 

Holubková, S. 3.7 

Honzíček, J. 2.58 

Hoza, A. 7.5 

Hoza, I. 2.21 

Hozlár, P. 2.38 

Hradilová, J. 2.73 

Hrádková, P. 1.41, 1.76, 2.106 

Hrdinová, J. 2.59, 2.79, 2.109 

Hrdý, R. 5.30 

Hredzák, S. 3.8, 4.9 

I. 

Hroboňová, K. 6.8 

Hroch, M. 1.17, 1.42 

Hrstka, M. 2.60, 2.119 

Hubálek, J. 5.30 

Humhal, F. 1.93 

Hynštová, K. 7.6 

I 

Ifrim, S. 2.89 

Ildiko, B. 2.110 

Illková, K. 2.61 

Iordache, A. 2.62, 2.63, 2.64, 4.6 

Iordache. A. 1.91 

Ivanová, S. 4.9 

J 

Jablonovska, K. 2.65 

Jablonovská, K. 1.81 

Jablonský, M. 3.7, 6.8, 6.9, 6.33, 7.12, 

7.34 

Jaffrezic-Renault, N. 5.36 

Jakabský, Š. 1.88, 1.115, 3.8 

Jakubíková, Z. 6.12 

Ján, T. 1.96 

Jančář, J. 1.108, 2.105, 6.37, 7.6, 7.11, 

7.13, 7.15, 7.19, 7.31, 7.33, 7.37, 

7.38 

Jančík, V. 7.30 

Jančovičová, V. 5.7, 5.12, 5.33, 5.34, 

6.12 

Jankulár, M. 1.43 

Jara Biedma, R. 2.51 

Jardim, C. M. 2.68 

Jarošová, A. 1.82, 2.77 

Jašek, O. 6.38 

Jašúrek, B. 5.21, 5.35 

Jedlička, P. 3.40 

Jedlovská, L. 1.112 

Jelénková, Z. 2.86 

Jenčárová, J. 1.57, 2.66

Ješko, D. 2.67 

Jham, G. N. 2.68 

Jirkovský, J. 5.13 

Jirků, V. 2.59, 2.79, 2.109 

Joanta, A. 2.23 

Jovancicevic, B. P.10 

Jovanovic, L. 1.55 

Juliš, M. 3.27 

Junák, J. 3.28 

K 

Kačániová, M. 1.24, 2.45, 2.69 

Kačík, F. 1.44 

Kačíková, D. 1.44 

Kalfus, J. 7.6, 7.11, 7.13 

Kaliňák, M. 6.11 

Kalvodová, J. 2.108 

Kaňuchová Pátková, J. 2.5, 2.32 

Kapoun, M. 1.46 

Karásek, P. 2.57 

Karásková, M. 6.38 

Karovičová, J. 2.112, 2.113 

Kašiarová, S. 1.9, 1.36 

Kassai, A. 1.22 

Kastori, R. 1.92 

Kaszuba, M. P.8 

Katuscak, S. 3.7 

Katuščák, S. 2.11, 2.91, 2.92, 6.8, 6.9, 

6.50, 7.7, 7.12, 7.34 

Kavřík, R. 2.107 

Kazíková, J. 6.9 

Kelar, L. 6.38 

Khan, M. S. A. 7.22 

Khlebnikova, N. 1.79 

Kikovic, D. 1.55 

Kirschnerová, S. 6.9, 7.7, 7.12, 7.34 

Kislinger, J. 6.10 

Kiss, S. A. 1.92 

Kizlink, J. 1.47, 4.19, 4.20 

Klakurková, L. 3.29 

Klakurková. L. 3.21 

Author Index 

Klánová, J. 1.5 

Klimko, T. 1.43 

Klímová, Z. 1.48 

Klučáková, M. 6.18, 6.34, 6.35, 6.36 

Kňazovická, V. 2.69 

Knobloch, J. 5.39 

Kočí, R. 2.44, 2.70, 2.87 

Kocourek, V. 1.40, 2.40, 2.58 

Kohajdová, Z. 2.112, 2.113 

Kohoutkova, J. 2.78 

Kohoutková, J. 2.71, 2.75 

Kolajova, Z. 2.21 

Kolářová, J. 2.72, 2.73, 2.74 

Kolman, V. 1.49 

Konečná, H. 2.55, 2.88 

Konupčík, P. 6.38 

Koplík, J. 3.43 

Koreňovská, M. 2.104 

Koryagina, N. 1.79 

Kosinková, J. 2.91 

Kostelanska, A. 2.78 

Kostelanska, M. 2.17 

Kostelanská, M. 2.75 

Kovačič, J. 7.20 

Kováčik, P. 1.50, 1.109 

Kováčová, M. 3.30 

Kovalczuk, T. P.7 

Kovaliková, N. 1.25, 1.51 

Kozdas, O. 3.13 

Kráčmar, S. 1.13 

Kraic, F. 2.12 

Kraic, J. 2.56 

Krajčírová, A. 2.114 

Krajcova, A. 2.76 

Kraková, T. 2.10 

Králiková, R. 1.52, 1.53 

Kramarova, D. 2.21, 2.22 

Krascsenits, Z. 1.113 

Krátká, L. 1.82, 2.77 

Krátký, J. 3.40 

Kratochvíla, J. 7.5 

Krčma, F. 6.41 

I.

Author Index 

Krejčová, H. 3.35 

Kristýna, U. 1.70 

Krivenko, P. P.4 

Křížová, J. 6.19 

Krouská, J. 6.29 

Kroutil, O. 6.37 

Krplova, A. 2.17, 2.78 

Krplová, A. 1.54, 2.71, 2.75 

Krulikovská, T. 2.59, 2.79, 2.109 

Kružlicová, D. 2.12, 2.80 

Krýsa, J. 5.14, 5.23 

Kubala, L. 2.100 

Kubátová, D. 3.12, 3.31 

Kubíčková, K. 1.101 

Kubincová, L. 1.34 

Kučera, F. 7.8, 7.23 

Kučera, J. 7.9, 7.32 

Kučerík, J. 2.117, 6.5, 6.10, 6.16, 6.25, 

6.27, 6.30, 6.34, 7.19 

Küchler, S. P.9 

Kukurová, K. 2.37, 2.38 

Kulacs, P. 2.123 

Kulilová, P. 6.29 

Kupka, D. 1.99, 3.10 

Kušnierová, M. 1.37, 1.56, 1.58 

L 

Lacina, O. 1.40, 1.54, 2.71, 2.122 

Lacombe, S. 5.10 

Lahučký, L. 1.94 

Lalevic, B. 1.55 

Lána, R. 1.13, 1.17, 1.38 

Lancová, K. 2.71 

Langová, L. 1.101 

Lankmayr, E. 2.80, 2.94 

Lansky, M. 2.122 

Laštovičková, M. 2.81, 2.82, 4.18 

Lauková, A. 1.116 

Laurová, M. 1.44 

Lazar, L. 6.38 

Lazor, P. 1.96 

I. 

Lehene, C. 2.62 

Lehkoživová, J. 2.113 

Lehotay, J. 6.8 

Lejeune, P. 5.10 

Lerche, D. P.9 

Letko, M. 4.10, 7.25 

Libardoni, M. P.7 

Lipenská, M. 1.104 

Liptaiová, D. 1.24 

Lojek, A. 2.100 

López-Barea, J. 2.50 

Loupancová, B. 2.127 

Lovas, M. 6.48 

Lovás, M. 1.115, 3.8, 3.30, 4.7 

Luptáková, A. 1.56, 1.57, 1.58, 2.66, 

3.24 

Lupu, D. 1.80, 2.28 

Luster, J. 1.85 

M 

Macák, M. 1.85 

Mach, V. 6.31 

Macharáčková, B. 2.126 

Machát, J. 1.5, 1.27, 1.98 

Mačingová, E. 1.57 

Macků, I. 2.127 

Mácová, D. 1.6, 1.28 

Macuchová, S. 2.29, 2.30, 2.31, 2.84 

Majzlík, P. 6.14, 6.39 

Malá, Z. 1.59 

Malenovská, M. 6.18 

Malyszová, J. 2.77 

Man, O. 3.29 

Manso, J. M. 1.71 

Marcu, A. 3.39 

Mareček, J. 2.85 

Mareš, J. 1.100 

Máriássyová, M. 2.45 

Marková, J. 2.71 

Marković, D. 1.60

Márová, I. 2.9, 2.39, 2.44, 2.55, 2.70, 

2.84, 2.87, 2.88, 2.100, 7.10, 7.26, 

7.27, 7.37 

Márová, i. 2.86 

Maršálek, B. 6.19 

Marták, J. 7.25 

Martincová, J. V. 1.61 

Marušicová, B. 6.44 

Masák, J. 2.59, 2.79, 2.109 

Mašek, I. 1.7, 1.23, 1.61, 1.63, 1.64, 

1.65 

Másilko, J. 3.41 

Mášová, M. 1.100 

Mastihuba, V. 2.10 

Mastihubová, M. 2.10 

Matalová, S. 2.97, 2.105 

Matea, C. T. 2.89 

Matik, M. 3.30 

Matoušek, J. 1.14, 1.66 

Matulová, D. 2.108 

Maxová, M. 2.117 

Mazanec, K. 2.90 

Mazur, M. 6.11 

Melušová, S. 7.26 

Merna, J. 7.21 

Mesaros, C. 4.6, 4.15 

Mesbah, A. W. 4.13 

Mihăiescu, R. 1.75 

Mihăiescu. T. 1.75 

Mika, O. 1.63 

Mika, O. J. 1.67 

Mikula, M. 5.17, 6.12, 6.40 

Mikulášik, R. 2.11, 2.91 

Mikulášik. R. 2.92 

Mikulíková, R. 2.29, 2.30, 2.31, 2.84, 

2.88 

Milata, V. 1.110, 2.11 

Miljkovic, B. 

Mindukshev, I. 1.8 

Minea, R. 2.62 

Mirzaei, M. 4.23 

Mládková, Z. 1.68 

Author Index 

Mocák, J. 2.12, 2.80, 2.94 

Močár, K. 1.24 

Mockovčiaková, A. 3.9, 3.19 

Mojtahedi, M. M. 4.22 

Mojtahedi. M. M. 4.13 

Momeu, C. 2.43 

Moos, M. 1.69, 1.70, 1.97 

Moreno, F. 1.71, 1.72, 2.93 

Mravcová, L. 1.6, 1.73, 1.84, 1.106, 

7.19 

Mravec, F. 6.13 

Mrázová, V. 2.12, 2.94 

Müller, L. 2.70 

Muller, P. 1.38 

Muntean, E. 1.74, 1.75 

Muntean, N. 1.74, 1.75 

Munteanu, M. F. 2.95 

Musilová, J. 1.94 

N 

Nápravníková, M. 1.41, 1.76, 2.106 

Narres, H.-D. 4.5 

Navrátil, J. 7.24 

Nemcová, K. 2.32 

Němec, K. 3.29 

Németh, L. 2.7 

Nešpůrek, S. 7.14, 7.24, 7.28 

Nesterenko, P. 1.15 

Nezbedová, E. 7.36 

Niksic, M. 1.55 

Noaman, M. S. M. 3.47 

Noskovič, J. 1.50 

Novák, F. 6.10 

Novák, J. 1.65 

Nováková, E. 4.10, 7.25 

Novotný, M. 7.27, 7.37 

Nôžková, J. 2.45, 2.69 

O 

Obruča, S. 7.10, 7.26, 7.27, 7.37 

Ohlidalová, M. 6.44 

I.

Author Index 

Omelka, L. 6.14, 6.39 

Omelková, J. 2.47, 2.61, 2.96, 2.97, 

2.105 

Ondruška, V. 7.10 

Opravil, T. 3.5, 3.12, 3.32, 3.33, 3.34, 

3.35, 3.36, 3.41, 3.42 

Orolínová, Z. 3.9, 3.10 

Ošmera, P. 6.15 

Ouzzane, I. 7.28 

P 

Pajurková, J. 6.41 

Pállová, Z. 3.10 

Palou, M. 3.47 

Panák, J. 5.31, 5.32 

Pařilová, K. 2.44, 2.55, 2.88 

Pauliková, A. 1.53 

Paulíková, A. 1.52 

Paulusová, H. 6.42 

Păun. N. 1.91 

Pavlisová, D. 5.27 

Pečinková, D. 2.73 

Pekař, M. 6.13, 6.28, 6.29, 6.34, 7.19 

Péruchon, L. 5.16 

Pesenjanski, I. 

Pešková, K. 1.68 

Peterka, F. 5.15 

Petriláková, K. 7.12, 7.34 

Petrovičová, M. 5.34, 6.42, 6.43, 6.44 

Piechová, J. 7.27, 7.37 

Pigeot-Remy, S. 5.10 

Pigot, T. 5.10 

Pilný, O. 6.36 

Pintea, A. 2.23, 2.28, 2.42, 2.99, 2.120, 

4.16 

Pleskačová, H. 1.84 

Pobudová, Z. 6.40 

Podborská, M. 2.100 

Podunavac-Kuzmanović, S. 2.102 

Podunavac-Kuzmanović, S. O. 2.101, 

2.103 

I.10 

Polívka, L. 2.123 

Polívka, Ľ. 1.77 

Polovka, M. 2.11, 2.13, 2.104, 2.118 

Polovka¨, M. 2.125 

Popelkova, M. 2.129 

Pospíšilová, Ľ. 6.32 

Pospíšilová, S. 3.27 

Poustka, J. 1.41, 1.54, 1.76, 2.17, 2.75, 

2.106 

Považanec, F. 6.9 

Přikryl, R. 7.29 

Princz, E. 1.92 

Prokeš, O. 1.93, 3.11 

Prónayová, N. 6.11 

Prudíková, I. 2.105 

Průšová, A. 6.16 

Ptáček, P. 3.6, 3.12, 3.31, 3.32, 3.33, 

3.34, 3.35, 3.36, 3.37, 3.41, 3.42, 

3.43 

Pueyo, C. 2.50 

Pulkrabova, J. 2.14 

Pulkrabová, J. 1.41, 1.76, 2.106 

Puzenat, E. 5.6, 5.11, 5.16, 5.36 

R 

Radilov, A. 1.8, 1.79 

Rădulescu, H. 1.92 

Raica, P. 3.46 

Raicevic, V. 1.55 

Rapta, P. 6.17, 6.39 

Recman, L. 7.11 

Reháková, M. 1.37, 5.7, 5.17, 5.27, 

5.32, 5.37 

Reinprecht, L. 4.19 

Rembovskiy, V. 1.8 

Remenárová, K. 5.21 

Richtera, L. 6.37, 7.30 

Ricking, M. 1.38 

Riddellova, K. 2.26, 2.36 

Rittich, B. 2.115, 2.116, 2.121 

Rogelj, I. P.3

Rolník, M. 7.31 

Rop, O. 2.21 

Rosická, Z. 1.64 

Ruprich, J. 2.72, 2.73, 2.74, 2.107, 

2.108 

Rusu, D. 3.39 

Rusu, M. 3.39 

Rutteová, A. 3.37 

Rychlý, J. 3.7 

Rýznarová, B. 7.9 

Ř 

Řeháková, J. 2.107, 2.108 

Řehůřková, I. 2.72, 2.73, 2.74, 2.107, 

2.108 

Řezníček, V. 2.125 

S 

Sadílek, J. 7.9, 7.32 

Saláková, S. 2.72, 2.73, 2.74 

Salyk, O. 5.24, 6.20, 6.46, 6.47 

Santos, J. H. 1.78 

Sarlea, M. M. 2.23 

Savelieva, E. 1.79 

Schreiberová, O. 2.59, 2.79, 2.109 

Schulzova, V. 2.76 

Schurek, J. 2.14, 2.122 

Schůrek, J. P.7, 1.16 

Schwarzbauer, J. P.5, 1.38 

Sedláček, P. 6.18 

Sharifi, A. 4.23 

Sharifi, R. 4.13 

Simon, S. 1.80 

Sisol, M. 1.102 

Skláršová, B. 2.16 

Skybova, M. 6.48 

Sladkova, P. 2.33 

Slančo, P. 1.11, 1.26 

Slánská, P. 7.23 

Sloviková, A. 7.33 

Author Index 

Snopkova, V. 1.99 

Snopková, V. 1.81 

Sobisch, T P.9 

Socaciu, C. 2.42, 2.43, 6.51 

Sommer, L. 1.69, 1.70, 1.97, 1.98, 

1.107 

Soran, M.-L. 2.110 

Stančík, J. 5.18, 5.38, 5.40 

Stancová, V. 1.82, 2.77 

Staněk, T. 3.6, 3.38 

Stanila, A. 3.39 

Stará, Z. 6.41 

Staško, A. 2.15 

Stefanovits-Bányai, É. 1.92 

Stejkora, J. 6.47 

Stepanov, B. 

Stopka, P. 1.89, 6.19 

Stoudkova, H. 2.130 

Stoupalová, M. 1.73, 1.84 

Stratilová, E. 2.47, 2.48 

Suchanová, M. 2.40 

Suchý, P. 1.82 

Suhaj, M. 2.13, 2.104 

Sulovský, P. 3.38 

Superatová, R. 6.14 

Surducan, E. 2.110 

Surducan, V. 2.110 

Svačina, Z. 7.21 

Svěrák, T. 3.13 

Světlík, J. 6.39 

Svoboda, J. 5.39 

Svoboda, L. 6.26 

Svoboda, Z. 2.29, 2.30, 2.31, 7.26 

Svobodová, D. 1.76 

Synek, P. 6.38 

Syrový, T. 5.21, 5.35 

Szitás, A. 6.9 

Szkandera, R. 1.31 

Szombathová, N. 1.85, 6.52 

I.11

Author Index 

Š 

Ščudlová, J. 7.38 

Šepelák, V. 1.58, 3.10, 3.30 

Šestinová, O. 1.11, 1.26, 1.35, 1.86, 

1.87 

Ševčíková, A. 2.100 

Šiler, P. 3.40 

Šima, J. 6.52 

Šimanský, V. 1.85, 1.95 

Šimko, P. 2.16 

Šimkovic, I. 2.97 

Šimon, P. 2.16, 2.92 

Šimonová, I. 2.112, 2.113 

Šindelář, V. 1.49, 4.24, 7.22 

Šiška, B. 2.52, 2.114 

Škarpa, P. 1.68 

Šlebodová, A. 2.15 

Šnirc, V. 6.17 

Šnuderl, K. 2.94 

Šopíková, M. 2.82 

Šoukal, F. 3.5, 3.6, 3.12, 3.32, 3.33, 

3.34, 3.35, 3.36, 3.41, 3.42, 3.43 

Špaldon, T. 1.11, 1.26, 1.35, 1.86, 1.87 

Španová, A. 2.115, 2.116, 2.121 

Štancl, M. 4.24 

Šťavíková, L. 2.118, 2.119 

Štefušová, K. 1.88 

Štekner, B. 7.20 

Štenclová, D. 1.100 

Števulová, N. 1.34, 1.89, 3.23, 3.24, 

3.28 

Štofan, D. 1.24 

Štolc, S. 6.17 

Štoudková, H. 2.117, 2.127 

Štyriaková, I. 1.90, 1.99, 2.65 

Šubrt, J. 5.20 

Šupinová, L. 1.103 

Šurina, I. 2.11, 2.91 

Šutriepka, M. 1.43 

Šutý, Š. 6.33, 7.12, 7.34 

Švajdlenková, H. 7.35 

I.12 

Švehla, B. 6.33 

T 

Tamaian, R. 1.91 

Taubert, L. 1.92 

Tenkrát, D. 1.93, 3.11 

Terpáková, E. 3.44 

Tiňo, R. 6.50, 7.25 

Tobiašová, E. 1.85, 1.95, 6.52 

Tobiášová, T. 1.28 

Tocháček, J. 7.13 

Toma, C. 2.95 

Toma, C.-C. 2.120 

Toman, P. 7.24 

Toman, R. 2.52, 2.114 

Tomancová, L. 3.38 

Tomáš, J. 1.94, 1.109 

Tomková, M. 2.87 

Tóth, T. 1.95, 1.96 

Trachtová, S. 2.121 

Trčková, M. 2.70 

Tremlová, B. 1.17 

Tria, J. 1.15 

Trif. M. 6.51 

Trna, J. 2.100 

Tudoran, C. 2.110 

Tuhovčáková, L. 1.32 

Turčániová, Ľ. 1.116 

Turianicová, E. 3.45 

Tycová, M. 2.116 

U 

Uhl, A. P.9 

Ungureşan, M. L. 2.42, 3.14, 3.46 

Urban, U. 2.60 

Urbánková, K. 1.97, 1.98 

Urbanova, J. 2.122 

Urbanová, J. 1.40, 1.54 

Ürgeová, E. 1.77, 2.123, 2.124

V 

Vaclavik, L. 2.17, 2.26 

Václavík, L. 1.16, 1.54 

Václavíková, M. 1.88 

Vaculík, M. 1.43 

Vadkertiová, R. 2.128 

Vajciková, I. 2.5, 2.32 

Vala, M. 4.14, 6.20, 6.21, 7.14, 7.24 

Valaseková, A. 6.12 

Valeš, M. 7.20 

Vališ, J. 5.21, 5.35 

Valko, M. 5.8, 6.11 

Válková, d. 6.7 

Valovičová, Z. 1.116 

Vašková, A. 1.90, 1.99, 3.10 

Vávrová, M. 1.6, 1.13, 1.17, 1.23, 1.32, 

1.42, 1.73, 1.84, 1.100, 1.101, 

1.106, 1.114, 7.19 

Večerek, V. 1.13, 1.42, 1.84, 1.101 

Veerecken, H. 4.5 

Veleba, O. 3.40 

Velebný, V. 6.13 

Vereš, J. 1.102 

Veselá, M. 1.103, 1.104, 5.22, 5.28 

Veselská, V. 1.105 

Veselý, M. 1.103, 1.104, 5.6, 5.9, 5.18, 

5.22, 5.24, 5.28, 5.29, 5.38, 5.40, 

6.31 

Veselý, P. 7.36 

Vespalcová, M. 2.57, 2.125 

Vicas, S. 2.43 

Vinter, V. 3.42 

Vítečková, H. 1.73, 1.106 

Vitkovič, M. 3.47 

Vitoulová, E. 2.126 

Vítová, E. 2.127, 2.129, 2.130 

Vizárová, K. 6.9, 7.7, 7.12, 7.34 

Vlach, B. 7.36 

Vlase, L. 2.95 

Vlčková, B. 2.61 

Vlčková, Z. 6.25, 6.27 

Author Index 

Vojta, Š. 1.107 

Vojtiskova, P. 2.22 

Vojtová, L. 1.108, 7.10, 7.19, 7.27, 7.33, 

7.37 

Vollmannová, A. 1.50, 1.96, 1.109 

Vondráčková, H. 2.87 

Vorlová, L. 2.27 

Vorozhtsova, M. 5.30 

Vránová, D. 2.125, 2.128 

Vrecková, Z. 1.110, 5.5, 5.8 

Vremeră, R. 1.91 

Vršecký, M. 3.32 

Vrška, M. 1.111, 4.10 

Vydrová, L. 1.106 

Vyňuchal, J. 6.46, 6.47 

W 

Ward, S. G. 1.78 

Weidlich, T. 5.21, 5.35 

Weiter, M. 4.14, 6.20, 6.21, 7.14, 7.24, 

7.28 

Y 

Youssef, C. 5.36 

Z 

Zachariasova, M. 2.17, 2.78 

Zachariášová, M. 2.75 

Zachová, L. 2.60 

Zajíčková, L. 6.38 

Zakova, Z. 2.76 

Zalibera, M. 6.17 

Zamazalová, P. 3.48 

Zamiri, B. 4.23 

Zaujec, A. 1.85, 1.112, 6.52 

Zdeňková, M. 2.128 

Zdráhal, Z. 2.55, 2.88 

Zechmiestrová, L. 2.96 

Zelenka, J. 6.26 

Zemanova, J. 2.129, 2.130 

I.1

Author Index 

Zemanová, J. 2.117, 2.127 

Zemanová, L. 1.113 

Zita, J. 5.23 

Zlámalová Gargošová, H. 1.33, 1.101, 

1.114 

Zmeškal, O. 4.14, 5.24, 6.20, 6.21, 

6.46, 7.24 

Zmrzlý, M. 3.49 

I.1 

Znamenáčová, I. 1.115 

Zrostlíková, J. P.7 

Zubrik, A. 1.116 

Zvezdanović, J. 1.60 

Ž 

Ždánská, P. 2.86 

Žídek, J. 7.15, 7.31, 7.38 

Žúbor, V. 6.11

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