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2. ENVIRONMENTAL ChEMISTRy & TEChNOLOGy 2.1. Lectures

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Chem. Listy, 102, s265–s1311 (2008) Environmental Chemistry & Technology<br />

tion of ecotoxicological parameters. Calculated values from<br />

probit model and experimental values were in very good correlation<br />

with declared value of tests.<br />

Table II<br />

The ecotoxicity of K 2 CrO 4 (n = 9; concentration in mg dm –3 )<br />

Daphtoxkit F Thamnotoxkit Lemma<br />

EC50/ EC50/ F minor<br />

24 h 48 h LC50/24 h IC50/168 h<br />

Declared<br />

values<br />

1.03 0.75 0.10 –<br />

Experim.<br />

value<br />

0.99 0.79 0.092 20.52<br />

Probit<br />

model<br />

0.96 0.69 0.093 –<br />

Median 0.99 0.75 0.093 20.00<br />

s 0.0286 0.0411 0.0035 0.5200<br />

Conclusions<br />

The ecotoxicity of the tested chemical was confirmed by<br />

the application of ecotoxicological tests. In the majority of<br />

cases, on the base of the obtained results of the study tests with<br />

chemical, the tests perform very suitable way for the study of<br />

ecotoxicity and can be used for evaluation of ecotoxicity of<br />

other chemicals and wastes. This study confirmed declared<br />

value and all data were statistical evaluated in detail, and the<br />

sensitivity of testing organism was also confirmed.<br />

This work has been supported by Project No. MSM<br />

002163050 form the Ministry of Education, Youth and Sports<br />

of the Czech Republic, and from the COST ACTION 636 Project<br />

No. OC 183.<br />

s360<br />

REFEREnCES<br />

1. UnECE:Globally Harmonized System of Classification<br />

and Labeling of Chemicals. ST/SG/AC.10/C.3/40/<br />

Add.221 December 2001.<br />

<strong>2.</strong> van Straalen n. M.: Environ. Toxicol. Pharmacol. 11,<br />

167 (2002).<br />

3. Isnard P., Flammarion P., Roman G. et all: Chemosphere<br />

45, 659 (2001).<br />

4. OECD Environmental health and Safety Publications:<br />

Series on testing and Assesssmnet no.10, EnV/MC/<br />

CHEM (98)18.<br />

5. OECD Environmental health and Safety Publications:<br />

OECD Guidelines for Testing Chemicals no 201 (2006,<br />

2004, 1992)<br />

6. ISO 8692:2004: Water quality – Freshwater algal<br />

growth inhibition test with unicellular green algae<br />

7. ISO 6341:1996: Water quality – Determination of the<br />

inhibition of the mobility of Daphnia magna Straus<br />

(Cladocera, Crustacea) – Acute toxicity test<br />

8. ISO 20079:2005: Water quality – Determination of the<br />

toxic effect of water constituents and waste water on<br />

duckweed (Lemna minor) – Duckweed growth inhibition<br />

test<br />

9. Commission of the European Communities, 1996. Technical<br />

guidance document in support of commission<br />

directive 93/67/EEC on risk assessment for new notified<br />

substances and commission regulation (EC) No 1488/94<br />

on risk assessment for existing substances. Part II;<br />

Environmental Risk Assessment. Office for official publications<br />

of the European Communities, Luxembourg.

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