2. ENVIRONMENTAL ChEMISTRy & TEChNOLOGy 2.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Environmental Chemistry & Technology
cross-linked by the EDC – nHS after the 78 days of incubation
in the physiological solution.
Fig. 4. Morphology of degraded collagen coated on the net and
cross-linked by the EDC – NhS
Conclusions
The all cross-linked collagen samples embodied very
good adhesion to the net when coated and did not show any
separation until the total degradation. Samples cross-linked
by the new system of EDC-nHS attained approximately the
same swelling ratio like samples cross-linked by the resins of
LYOFIX and MH-83. The biggest strength of polymeric net
and hydrolytic degradation resistivity showed sample crosslinked
by the EDC-nHS system, which were stable against
s518
the degradation for 78 days in physiological solution at 37 °C
(confirmed by microscopy). Due to the obtained properties
the new cross-linked collagen might enhance the biocompatibility
of commercially used net CHS 100 since it is coated
and implanted. Moreover, collagen itself might be modified
(e.g. with hyaluronic acid) in order to speed up the wound
healing process or reduce the tissue inflammation in the net
surrounding environment.
This work was supported by the Ministry of Education,
Youth and Physical Training of the Czech Republic under the
research project MSM 0021630501
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