2. ENVIRONMENTAL ChEMISTRy & TEChNOLOGy 2.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Environmental Chemistry & Technology
Results
All tetracyclines have typical absorption spectrum. They
have two characteristical absorption maximas, 264 nm and
360 nm. Due to their characteristic we could determinate
tetracyclines and their metabolites together by spectrophotometry.
Inlet concentrations of analytes were always higher than
outlet concentrations. This indicates that these pharmaceuticals
are removed partially in the wastewater treatment plant.
The change of tetracycline concentration may be caused by
photodegradation.
Conclusions
As the use of pharmaceuticals is increasing, fast sample
preparation and determination method is required.
Samples were taken from wastewater treatment plant
placed in Veterinary and Pharmaceutical university Brno
campus, from inlet and outlet.
s512
Samples were filtered before extraction.
SPE was optimized for preparation and preconcentration
of wastewater samples.
An efficient method for determination of tetracyclines in
wastewater, using spectrophotometry, was developed.
Spectrophotometry was used because this is commonly
used in laboratories and machine operation is cheap.
This work has been supported by grant COST, action
636, project no. OC-183.
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