2. ENVIRONMENTAL ChEMISTRy & TEChNOLOGy 2.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Environmental Chemistry & Technology
bacterially treated “stabilizate” and “stabilizate” of abiotic
control are shown in Figs. 2.–4. They show the significant
qualitative changes in the “stabilizate” initiated by SRB. The
sulfates from the original major component of “stabilizate”
– CaSO 4 . 2H2 O (Fig. 2.) – were reduced according by SRB
forming hydrogen sulfide as proved by the sensorial detection
of classical strong H 2 S smell. This is indirectly confirmed by
Fig. 3, which proves the extinction of CaSO 4 and formation
of CaCO 3 according to equation (1):
SRB
2C 3 H 5 O 3 na + CaSO 4 → 2CH 3 COOna + CO 2 +
CaCO 3 + H 2 S + H 2 O
The above-mentioned changes have not been observed
in abiotic control as documented by the comparison of qualitative
X-ray analysis of “stabilizate” before applying SRB
(Fig. 2.) with the qualitative X-ray analysis of “stabilizate”
in abiotic control (Fig. 4.). The results of energy dispersive
spectrometry (EDS) analysis and scanning electron microscope
of original “stabilizate”, bacterially treated “stabilizate”
and “stabilizate” of abiotic control are shown in Figs. 5.–7.
They fall into line with Figs. 2., 3. and 4. and suggest on the
Fig. 1. Changes of sulfates concentration during the discontinuous
cultivation of sulfate-reducing bacteria.
c – sulfates concentration, t – time of sulfate-reducing bacteria
cultivation, – “stabilizate” with SRb, – “stabilizate” without
SRb (abiotic control)
Fig. 2. Qualitative x-ray analysis of “stabilizate” before application
of sulfate-reducing bacteria. I – intenzity, u – 2-theta
uhol, 1 – CaSO 4 . h2 O, 2 – SiO 2
(1)
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elimination of CaSO 4 . 2H2 O form “stabilizate”. The element
composition of solid phases (Figs. 5., 6. and 7.) accepts with
this fact.
Fig. 3. Qualitative x-ray analysis of solid phase formed by the
effect of SRb on “stabilizate”. I – intenzity, u – 2-theta uhol,
1 – CaCO 3 , 2 – SiO 2
Fig. 4. Qualitative x-ray analysis of solid phase in abiotic con-
Fig. 5. EDS qualitative analysis of “stabilizate” before application
of sulfate-reducing bacteria