Argininosuccinate Lyase protein (ab113605) - Abcam
Argininosuccinate Lyase protein (ab113605) - Abcam
Argininosuccinate Lyase protein (ab113605) - Abcam
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Product Datasheet<br />
<strong>Argininosuccinate</strong> <strong>Lyase</strong> <strong>protein</strong><br />
<strong>ab113605</strong><br />
1 Image<br />
Overview<br />
Product name <strong>Argininosuccinate</strong> <strong>Lyase</strong> <strong>protein</strong><br />
Uniprot accession P04424<br />
Description<br />
Nature Recombinant<br />
Source E. coli<br />
Amino Acid Sequence<br />
Sequence MGSSHHHHHHSSGLVPRGSHMASESGKLWGGRFVGAVDPI<br />
MEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEM<br />
DQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELI<br />
GATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELI<br />
RTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVAL<br />
TRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAEL<br />
NFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDL<br />
ILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKA<br />
GRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMS<br />
AVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRK<br />
GMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLF<br />
SGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQ AQQA<br />
Molecular weight 53.80<br />
Start 1<br />
End 464<br />
Length 464<br />
Tags His<br />
Specifications<br />
Our Abpromise guarantee covers the use of <strong>ab113605</strong> in the following tested applications.<br />
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end<br />
user.<br />
Applications SDS-PAGE<br />
Purity > 95 % by SDS-PAGE.<strong>ab113605</strong> was purified using conventional chromatography techniques.<br />
Form Liquid<br />
Preparation and Storage<br />
Stability and Storage Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze /<br />
http://www.abcam.com/<strong>Argininosuccinate</strong>-<strong>Lyase</strong>-<strong>protein</strong>-<strong>ab113605</strong>.html 1
Product Datasheet<br />
General info<br />
Alternative names Argininosuccinase<br />
<strong>Argininosuccinate</strong> lyase<br />
Arginosuccinase<br />
ARLY_HUMAN<br />
ASAL<br />
ASL<br />
EC 4.3.2.1<br />
Pathway Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl<br />
phosphate: step 3/3.<br />
Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-Larginino)succinate:<br />
step 1/1.<br />
Involvement in disease Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900].<br />
Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is<br />
characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle<br />
hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and<br />
episodic unconsciousness.<br />
Sequence similarities Belongs to the lyase 1 family. <strong>Argininosuccinate</strong> lyase subfamily.<br />
Post-translational<br />
modifications<br />
thaw cycles.<br />
Target information above from: UniProt accession P04424 The UniProt Consortium<br />
The Universal Protein Resource (UniProt) in 2010<br />
Nucleic Acids Res. 38:D142-D148 (2010) .<br />
<strong>Argininosuccinate</strong> <strong>Lyase</strong> <strong>protein</strong> images<br />
SDS-PAGE - <strong>Argininosuccinate</strong> <strong>Lyase</strong><br />
<strong>protein</strong> (<strong>ab113605</strong>)<br />
pH: 8.00<br />
Constituents: 0.32% Tris HCl, 0.04% DTT, 10% Glycerol, 0.58% Sodium chloride<br />
Acetylation modifies enzyme activity in response to alterations of extracellular nutrient<br />
availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose<br />
increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-<br />
288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.<br />
15% SDS-PAGE showing <strong>ab113605</strong> (3 µg)<br />
at approximately 53.8 kDa.<br />
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"<br />
Our Abpromise to you: Quality guaranteed and expert technical support<br />
As standard<br />
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Product Datasheet<br />
Replacement or refund for products not performing as stated on the datasheet<br />
Valid for 12 months from date of delivery**<br />
Response to your inquiry within 24 hours<br />
In addition<br />
We provide support in Chinese, English, French, German, Japanese and Spanish<br />
Extensive multi-media technical resources to help you<br />
We investigate all quality concerns to ensure our products perform to the highest standards<br />
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,<br />
please visit http://www.abcam.com/abpromise or contact our technical team.<br />
Terms and conditions<br />
Guarantee only valid for products bought direct from <strong>Abcam</strong> or one of our authorized distributors<br />
**Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand,<br />
which operate a 120 day guarantee. Please contact your regional office for further details<br />
Antibodies tested only in recombinant samples are not covered for use on endogenous samples. Please check the datasheet for<br />
information on tested species<br />
"Predicted to react" information on datasheets is based on sequence homology and provided for reference only<br />
"Predicted to react" species are not guaranteed, as they have not been tested<br />
Fast Track products are not covered under the Abpromise. Please see Fast Track terms and condition for additional information<br />
<strong>Abcam</strong> biochemicals are novel compounds and we have not tested their biological activity in house. Please use the literature to identify<br />
how to use these products effectively. If you require further assistance please contact the scientific support team<br />
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