PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...

FSB1 – 2004
Food Science and Biotechnology in Developing Countries
The specific protease activity in the callus medium (extracellular enzyme) was
different for each growth-regulator combination (Figure 2). The maximal value of
specific activity of callus was obtained with Picloram and BAP 0.5 mg l -1 . And these
calli were selected for transfer to liquid medium to form cells suspension cultures.
The results indicated that callus and biomass from cell suspension culture contain
higher concentration of intracellular proteases than extracellular proteases (Figure
2). However the extracellular protease is more attractive with a view to
biotechnological production. The extracellular protease activities of free cell culture
medium are shown in the figure 3.
The enzyme activity directly correlated with the biomass production (date not
shown). In addition the extracellular protease reached the maximal value after 13
weeks decreasing subsequently to lower value (Figure 3).
Proteolytic activity was showed in agar medium were calli grown (Figure 2).
UT/L
18000
16000
14000
12000
10000
8000
6000
4000
2000
0
MS4 MS5 MS8 MS12 CALLUS
Murashige & Skoog Medium
Figure 2. Extracellular and intracellular (calli in MS12) protease activity of calli of
B. hemisphaerica grown in different growth regulators proportion, Auxins 0.5 mg l -1 .
Activity expressed in terms of tyrosine (UT/L).
Cell suspension culture medium had extacellular proteolytic activity of 2000 –
20000 UT/L (Figure 3).