PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
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FSB1 – 2004<br />
Food Science and Biotechnology in Developing Countries<br />
Cumulative Weight Fraction (W)<br />
1.2<br />
1<br />
0.8<br />
0.6<br />
0.4<br />
0.2<br />
0<br />
1.00E+03 1.00E+04 1.00E+05 1.00E+06 1.00E+07<br />
Molecular Weight (g/mol)<br />
O-Pec<br />
U-Pec<br />
B-Pec<br />
Figure 3. Cumulative weight fraction plotted against molecular weight of unfractionated pectin samples.<br />
(O-Pec): original pectin. (B-Pec): SP-unbound and HP bound pectin. (U-Pec): SP-unbound and HP<br />
unbound pectin.<br />
Table 1. The summary of chemical properties of O-Pec, B-Pec, and U-Pec by Valencia PME a .<br />
% DE a<br />
Mw<br />
(g/mol)<br />
Polydispersity<br />
(Mw/Mn)<br />
ζ-potential<br />
(mV)<br />
O-Pec 73 134,000 ± 3,439 1.96 -21.36 ± 0.14<br />
B-Pec 63 132,250 ± 3,889 2.13 -30.10 ± 1.62<br />
U-Pec 61 133,850 ± 3,748 2.11 -39.67 ± 1.05<br />
a % DE from NMR spectra. Coefficient of variation ranged from 0.23 to 3.96% for 2 to 4 replicates. Mw:<br />
weight average molecular, Mn: number average molecular weight
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