PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
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Food Science and Biotechnology for Developing Countries.2004<br />
2<br />
8<br />
0<br />
n<br />
m<br />
A<br />
b<br />
s<br />
I II<br />
III<br />
IV<br />
V<br />
No. Fracción<br />
No. Fraction<br />
FIGURE 1.<br />
Chromatography profile of the elution of 2X<br />
extract<br />
of J. mexicana in Econo-pac® High S, 0.05 M<br />
phosphate<br />
buffer , pH 6.3 and NaCl 0.0-1.0 M linear gradient.<br />
1<br />
.0<br />
N<br />
a<br />
C<br />
l M 0<br />
.0<br />
-<br />
m<br />
n<br />
0<br />
8<br />
2<br />
s<br />
A<br />
III<br />
Fracción<br />
No. Fracción<br />
No.<br />
FIGURE 2.<br />
12.5% Poliacrilamide gel electrophoresis of P-III:<br />
lane 1, P-III in Econo-pac Methyl, lane 2: P-III<br />
rec in Econo-pac High S, lane 3: P-IV<br />
M<br />
.0<br />
-0<br />
.5<br />
O<br />
2S<br />
4) 4<br />
1<br />
O<br />
2S<br />
4) 4<br />
1<br />
H<br />
(N