PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
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FSB1 – 2004<br />
Food Science and Biotechnology in Developing Countries<br />
TANIC (g/L)<br />
14<br />
12<br />
10<br />
8<br />
6<br />
4<br />
2<br />
0<br />
Table 1. Comparison in the production of tannase in liquid<br />
and solid cultures at 30º C (30 h).<br />
Strain<br />
Cultures<br />
Kind<br />
GH1 SmC<br />
GH1 SSC<br />
*SmC: Submerged Culture.<br />
*SSC: Solid State Culture.<br />
0 8 16 24 32 40 48 56 64 72 80 88<br />
TIME (h)<br />
Figure 1. Substrate uptake by A.<br />
niger at 30° C in SSC with 12.5g/L<br />
of tannic acid.<br />
Proteasse Activity U/ L*min<br />
10<br />
9<br />
8<br />
7<br />
6<br />
5<br />
4<br />
3<br />
2<br />
1<br />
0<br />
Tannase U/L Total Tannase U/L<br />
537 537<br />
2291 2291<br />
Tannase Activity (U/L)<br />
1800<br />
1600<br />
1400<br />
1200<br />
1000<br />
800<br />
600<br />
400<br />
200<br />
0 6 12 18 24 30 36 42 48<br />
Time (hours)<br />
Figure 3. Protease activity of GH1<br />
strain at 30° C in SSC with<br />
concentrations of 25(♦) y 50 (■) g/L<br />
of tannic acid.<br />
0<br />
0 6 12 18 24 30 36 42 48<br />
hours<br />
Figure 2. Tannase enzyme expression<br />
by the GH1 strain at 30° C in SSC<br />
with concentrations of 25(♦) and 50<br />
(■) g/L of tannic acid.