PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
PROTEASES FROM CELL CULTURE OF Bromelia hemisphaerica ...
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FSB1 – 2004<br />
Food Science and Biotechnology in Developing Countries<br />
RESULTS AND DISCUSSION<br />
Induction experiments: Figure 1 shows the results obtained for enzymatic activities at several<br />
inducer concentrations through 72 hours of culture.<br />
Activity (U/L*min)<br />
2500<br />
2000<br />
1500<br />
1000<br />
500<br />
0<br />
Conc 6.25 Conc 12.5 Conc 25<br />
Conc 50 Conc 100<br />
0 12 24 36 48 60 72<br />
Tme (Hours)<br />
Figure 1. Invertase production at several sucrose concentrations.<br />
Maximal invertase production was reached in cultures with 50 g/L at 60 hours. Results obtained<br />
were higher than those reported by Ashokkumar et al (2001).<br />
Repression experiments: Figure 2 shows the results obtained for enzymatic activities using<br />
the same inducer concentration (50 g/L) and several glucose concentrations through 72 hours<br />
of culture.<br />
Activity (U/L*min)<br />
45000<br />
40000<br />
35000<br />
30000<br />
25000<br />
20000<br />
15000<br />
10000<br />
5000<br />
conc 0 conc 6,25 conc 12,5<br />
conc 25 conc 50 conc 100<br />
0<br />
0 12 24 36 48 60 72 84 96<br />
Time (Hours)<br />
Figure 2. Invertase production at several glucose concentrations and 25 g/L of sucrose.