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CHAPTER 3.1 than at least they should be shared. After all, measuring by two standards might result in conflicting situations. 84 In conclusion, different technical settings results in different CASA motility outcomes, although results correlate well. References Arunvipas P., VanLeeuwen J.A., Dohoo I.R., Keefe G.P. 2003.Evaluation of the reliability and repeatability of automated milk urea nitrogen testing. Canadian Journal of Veterinary Research 67:60-63. Bland J.M., Altman D.G. 1986. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1:307-310. Loomis P.R. 2001. The equine frozen semen industry. Animal Reproduction Science 68:191-200. Loomis P.R., Graham J.K. 2008. Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Animal Reproduction Science 105:119-128. Ortega-Ferrusola C., Macías García B., Suárez Rama V., Gallardo-Bolaños J.M., González-Fernández L., Tapia J.A., Rodriguez-Martinez H., Peña F.J. 2009. Identification of sperm subpopulations in stallion ejaculates: changes after cryopreservation and comparison with traditional statistics. Reproduction in Domestic Animals 44:419-423. Vidament M., Vincent P., Martin F.X., Magistrini M., Blesbois E. 2009. Differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not. Animal Reproduction Science 112:22-35. Waite J.A., Love C.C., Brinsko S.P., Teague S.R., Salazar J.L. Jr., Mancil S.S., Varner D.D. 2008. Factors impacting equine sperm recovery rate and quality following cushioned centrifugation. Theriogenology 70:704-714. WBFSH. 2010 http://www.wbfsh.com/files/Semen%20standards.pdf.
3.2 Counting chamber type influences equine semen CASA motility outcomes Hoogewijs M., De Vliegher S., Govaere J., De Schauwer C., de Kruif A., Van Soom A. submitted 85
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AUTOMATED AND STANDARDIZED ANALYSIS
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AUTOMATED AND STANDARDIZED ANALYSIS
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A Area AI Artificial Insemination A
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PREFACE The first recorded case of
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GENERAL INTRODUCTION CHAPTER 1 3
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1.1.1. Introduction CHAPTER 1.1 The
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1.1.4. Concentration CHAPTER 1.1 Ac
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Fluorescent Activated Cell Sorter C
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CHAPTER 1.1 preparation (D, µm) is
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CHAPTER 1.1 These chambers are freq
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CHAPTER 1.1 it is not possible to o
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CHAPTER 1.1 overview of common abno
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CHAPTER 1.1 head, (B5) pyriform hea
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CHAPTER 1.1 The acrosome can be eva
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CHAPTER 1.1 The sperm chromatin str
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Collection and processing of equine
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CHAPTER 1.2 30 (a) Colorado State U
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CHAPTER 1.2 can be induced pharmaco
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Page 56 and 57: CHAPTER 1.2 48 Cryopreservation - t
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Page 67 and 68: CHAPTER 1 Flipse R.J., Patton S., A
Page 69 and 70: CHAPTER 1 Kuisma P., Andersson M.,
Page 71 and 72: CHAPTER 1 Medeiros A.S.L., Gomes G.
Page 73 and 74: CHAPTER 1 Pickett B.W. 1993. Collec
Page 75 and 76: CHAPTER 1 Taberner E., Morató R.,
Page 77: CHAPTER 1 Waite J.A., Love C.C., Br
Page 81: CHAPTER 2 As evidenced in the gener
Page 85: 3.1 Influence of technical settings
Page 88 and 89: CHAPTER 3.1 a CEROS (Hamilton-Thorn
Page 90 and 91: CHAPTER 3.1 Progressive Motility CA
Page 95 and 96: 3.2.1. Abstract CHAPTER 3.2 Sperm m
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Page 101 and 102: CHAPTER 3.2 Finally, Pearson coeffi
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Page 109 and 110: Table 5. Averages (± standard erro
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Page 119 and 120: 3.3.1. Abstract CHAPTER 3.3 Routine
Page 121 and 122: CHAPTER 3.3 morphological abnormali
Page 123 and 124: CHAPTER 3.3 The extended semen was
Page 125 and 126: CHAPTER 3.3 Table 1: Average values
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Page 129: References CHAPTER 3.3 Arunvipas P.
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Page 135 and 136: CHAPTER 3.4 therefore in these expe
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Page 139 and 140: 3.4.4.2. Experiment 2 CHAPTER 3.4 F
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4.1 Influence of different centrifu
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CHAPTER 4.1 4.1.2. Introduction 140
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CHAPTER 4.1 142 4.1.3.3. Semen proc
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CHAPTER 4.1 144 In the experiment 3
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CHAPTER 4.1 centrifugation (T1) and
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CHAPTER 4.1 (non CP vs CP) was sign
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35 B 80 A 33 beat cross frequency (
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CHAPTER 4.1 152 4.1.4.2. EXPERIMENT
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CHAPTER 4.1 Love and coworkers (200
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CHAPTER 4.1 Love C.C., Brinsko S.P.
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4.2.1. Abstract CHAPTER 4.2 The inc
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4.2.3. Materials and methods 4.2.3.
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CHAPTER 4.2 For the SLC group, 15 m
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CHAPTER 4.2 adding 600 µL of AO st
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4.2.3. Results 4.2.3.1. Sperm quali
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CHAPTER 4.2 The aforementioned “T
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CHAPTER 4.2 Sperm yields markedly d
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References CHAPTER 4.2 Barth A.D.,
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CHAPTER 4.2 Waite J.A., Love C.C.,
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CHAPTER 5 This thesis originated fr
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CHAPTER 5.1 Fig. 1. Schematic prese
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CHAPTER 5.1 higher than the current
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CHAPTER 5.1 al., 2007) showing a cl
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CHAPTER 5.1 treatment strategy for
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CHAPTER 5.2 supernatant allowing sp
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CHAPTER 5.2 such keeping the pellet
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CHAPTER 5.2 components of the semin
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Final reflections 5.4 Actual change
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CHAPTER 5 Dillon R.H., Fauci L.J.,
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CHAPTER 5 Suárez S.S., Osman R.A.
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SUMMARY technique that enables proc
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SUMMARY from stallions previously c
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SAMENVATTING beweeglijkheid (Hoofds
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SAMENVATTING de spermacellen gesele
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DANKWOORD Het obligatoire dankwoord
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DANKWOORD dankzij jou en ze zijn er
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CURRICULUM VITAE Maarten Hoogewijs
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BIBLIOGRAPHY Govaere J., Ducatelle
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BIBLIOGRAPHY ABSTRACTS AND PROCEEDI
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FUNDAMENTAL ASPECTS OF CRYOPRESERVA
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ADDENDUM I presence of these CPAs a
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ADDENDUM I Fig. 3. Theoretical curv
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ADDENDUM II DETAILED LOADING TECHNI
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In m’n hoofd is alles heel eenvou
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