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Totoal Motility CASA2 (%)
100
80
60
40
20
0
CHAPTER 3.1
Fig. 2. Scatter plots and corresponding Bland-Altman plots for agreement of total and progressive
motility obtained with CASA using settings 1 (CASA1, see text for details) vs. settings 2
(CASA2, see text for details) for frozen-thawed equine semen ( line of perfect
agreement, regression line fit the actual data).
3.1.5. Discussion
0 20 40 60 80 100
Total Motility CASA1 (%)
The importance of indicating which CASA settings are used when assessing the quality of
frozen-thawed semen is clearly demonstrated. The settings used here resulted in differences with a
potential important clinical and financial impact. Still, even more extreme settings have been
reported in literature (Ortega-Ferrusola et al., 2009; Vidament et al., 2009). However, results
obtained with both settings showed a very good agreement. This might enable laboratories to relate
their own findings with other reports. Depending on the settings used, an ejaculate might be
discarded or accepted for use in an AI program. So if the same standards are not uniformly applied,
Progressive Motility CASA2 (%)
80
60
40
20
0
0 20 40 60 80
Progressive Motility CASA1 (%)
83