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CHAPTER 3.1 Progressive Motility CASA1 T2 (%) Fig. 1. Scatter plots and corresponding Bland-Altman plots for analysis of repeatability of progressive motility obtained with CASA using settings 1 (CASA1, see text details) and settings 2 (CASA2, see text for details), respectively at time 1 (T1) vs. time 2 (T2) for frozenthawed equine semen ( line of perfect agreement, regression line fit the actual data). 82 60 40 20 0 0 20 40 60 Progressive Motility CASA1 T1 (%) Progressive Motility CASA2 T2 (%) 60 40 20 0 0 20 40 60 Progressive Motility CASA2 T1 (%)
Totoal Motility CASA2 (%) 100 80 60 40 20 0 CHAPTER 3.1 Fig. 2. Scatter plots and corresponding Bland-Altman plots for agreement of total and progressive motility obtained with CASA using settings 1 (CASA1, see text for details) vs. settings 2 (CASA2, see text for details) for frozen-thawed equine semen ( line of perfect agreement, regression line fit the actual data). 3.1.5. Discussion 0 20 40 60 80 100 Total Motility CASA1 (%) The importance of indicating which CASA settings are used when assessing the quality of frozen-thawed semen is clearly demonstrated. The settings used here resulted in differences with a potential important clinical and financial impact. Still, even more extreme settings have been reported in literature (Ortega-Ferrusola et al., 2009; Vidament et al., 2009). However, results obtained with both settings showed a very good agreement. This might enable laboratories to relate their own findings with other reports. Depending on the settings used, an ejaculate might be discarded or accepted for use in an AI program. So if the same standards are not uniformly applied, Progressive Motility CASA2 (%) 80 60 40 20 0 0 20 40 60 80 Progressive Motility CASA1 (%) 83
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AUTOMATED AND STANDARDIZED ANALYSIS
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AUTOMATED AND STANDARDIZED ANALYSIS
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A Area AI Artificial Insemination A
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PREFACE The first recorded case of
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GENERAL INTRODUCTION CHAPTER 1 3
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1.1.1. Introduction CHAPTER 1.1 The
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1.1.4. Concentration CHAPTER 1.1 Ac
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Fluorescent Activated Cell Sorter C
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CHAPTER 1.1 preparation (D, µm) is
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CHAPTER 1.1 These chambers are freq
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CHAPTER 1.1 it is not possible to o
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CHAPTER 1.1 overview of common abno
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CHAPTER 1.1 head, (B5) pyriform hea
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CHAPTER 1.1 The acrosome can be eva
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CHAPTER 1.1 The sperm chromatin str
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Collection and processing of equine
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CHAPTER 1.2 30 (a) Colorado State U
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Page 42 and 43: CHAPTER 1.2 centrifugation time and
Page 44 and 45: CHAPTER 1.2 36 → Colloid centrifu
Page 46 and 47: CHAPTER 1.2 of a stallion stored fo
Page 48 and 49: CHAPTER 1.2 1.2.4. Cryopreservation
Page 50 and 51: CHAPTER 1.2 0.5 mL straws, and glyc
Page 52 and 53: CHAPTER 1.2 determined if the final
Page 54 and 55: CHAPTER 1.2 46 Chemically defined e
Page 56 and 57: CHAPTER 1.2 48 Cryopreservation - t
Page 59 and 60: 1.3.1. Standards for equine AI dose
Page 61 and 62: CHAPTER 1.3 The use of density grad
Page 63 and 64: CHAPTER 1 Avery B., Greve T. 1995.
Page 65 and 66: CHAPTER 1 Cheng F.-P., Fazeli A., V
Page 67 and 68: CHAPTER 1 Flipse R.J., Patton S., A
Page 69 and 70: CHAPTER 1 Kuisma P., Andersson M.,
Page 71 and 72: CHAPTER 1 Medeiros A.S.L., Gomes G.
Page 73 and 74: CHAPTER 1 Pickett B.W. 1993. Collec
Page 75 and 76: CHAPTER 1 Taberner E., Morató R.,
Page 77: CHAPTER 1 Waite J.A., Love C.C., Br
Page 81: CHAPTER 2 As evidenced in the gener
Page 85: 3.1 Influence of technical settings
Page 88 and 89: CHAPTER 3.1 a CEROS (Hamilton-Thorn
Page 92 and 93: CHAPTER 3.1 than at least they shou
Page 95 and 96: 3.2.1. Abstract CHAPTER 3.2 Sperm m
Page 97 and 98: CHAPTER 3.2 depth but not the chamb
Page 99 and 100: 3.2.3.3. The different chambers and
Page 101 and 102: CHAPTER 3.2 Finally, Pearson coeffi
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Page 105 and 106: ISAS20rM Makler10r WHO 120 120 120
Page 107 and 108: 60 PM Rapid 50 40 30 20 10 0 CHAPTE
Page 109 and 110: Table 5. Averages (± standard erro
Page 111 and 112: CHAPTER 3.2 sperm (tail) which coul
Page 113 and 114: CHAPTER 3.2 Hoogewijs M.K., Govaere
Page 115: CHAPTER 3.2 Spizziri B.E., Fox M.H.
Page 119 and 120: 3.3.1. Abstract CHAPTER 3.3 Routine
Page 121 and 122: CHAPTER 3.3 morphological abnormali
Page 123 and 124: CHAPTER 3.3 The extended semen was
Page 125 and 126: CHAPTER 3.3 Table 1: Average values
Page 127 and 128: Repeatability Agreement SQA-Ve PM (
Page 129: References CHAPTER 3.3 Arunvipas P.
Page 133 and 134: 3.4.1. Abstract CHAPTER 3.4 In this
Page 135 and 136: CHAPTER 3.4 therefore in these expe
Page 137 and 138: CHAPTER 3.4 The averages obtained a
Page 139 and 140: 3.4.4.2. Experiment 2 CHAPTER 3.4 F
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References CHAPTER 3.4 Arunvipas P.
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4.1 Influence of different centrifu
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CHAPTER 4.1 4.1.2. Introduction 140
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CHAPTER 4.1 142 4.1.3.3. Semen proc
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CHAPTER 4.1 144 In the experiment 3
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CHAPTER 4.1 centrifugation (T1) and
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CHAPTER 4.1 (non CP vs CP) was sign
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35 B 80 A 33 beat cross frequency (
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CHAPTER 4.1 152 4.1.4.2. EXPERIMENT
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CHAPTER 4.1 Love and coworkers (200
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CHAPTER 4.1 Love C.C., Brinsko S.P.
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4.2.1. Abstract CHAPTER 4.2 The inc
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4.2.3. Materials and methods 4.2.3.
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CHAPTER 4.2 For the SLC group, 15 m
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CHAPTER 4.2 adding 600 µL of AO st
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4.2.3. Results 4.2.3.1. Sperm quali
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CHAPTER 4.2 The aforementioned “T
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CHAPTER 4.2 Sperm yields markedly d
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References CHAPTER 4.2 Barth A.D.,
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CHAPTER 4.2 Waite J.A., Love C.C.,
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CHAPTER 5 This thesis originated fr
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CHAPTER 5.1 Fig. 1. Schematic prese
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CHAPTER 5.1 higher than the current
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CHAPTER 5.1 al., 2007) showing a cl
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CHAPTER 5.1 treatment strategy for
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CHAPTER 5.2 supernatant allowing sp
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CHAPTER 5.2 such keeping the pellet
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CHAPTER 5.2 components of the semin
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Final reflections 5.4 Actual change
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CHAPTER 5 Dillon R.H., Fauci L.J.,
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CHAPTER 5 Suárez S.S., Osman R.A.
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SUMMARY technique that enables proc
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SUMMARY from stallions previously c
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SAMENVATTING beweeglijkheid (Hoofds
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SAMENVATTING de spermacellen gesele
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DANKWOORD Het obligatoire dankwoord
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DANKWOORD dankzij jou en ze zijn er
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CURRICULUM VITAE Maarten Hoogewijs
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BIBLIOGRAPHY Govaere J., Ducatelle
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BIBLIOGRAPHY ABSTRACTS AND PROCEEDI
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FUNDAMENTAL ASPECTS OF CRYOPRESERVA
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ADDENDUM I presence of these CPAs a
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ADDENDUM I Fig. 3. Theoretical curv
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ADDENDUM II DETAILED LOADING TECHNI
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In m’n hoofd is alles heel eenvou
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